Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0685360 | KEH30332.1 | 88.112 | 286 | 20 | 2 | 19 | 290 | 12 | 297 | 2.27e-169 | 481 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0685360 | sp|Q9SZL8|FRS5_ARATH | 54.630 | 108 | 45 | 2 | 108 | 211 | 72 | 179 | 2.86e-23 | 102 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0685360 | A0A072UKW2 | 88.112 | 286 | 20 | 2 | 19 | 290 | 12 | 297 | 1.09e-169 | 481 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0685360 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0037880 | Msa0685360 | 0.811019 | 8.630993e-51 | -8.615850e-47 |
| Msa0607540 | Msa0685360 | 0.802887 | 4.531857e-49 | -8.615850e-47 |
| Msa0647830 | Msa0685360 | 0.835508 | 1.611315e-56 | -8.615850e-47 |
| Msa0685360 | Msa0688070 | 0.813645 | 2.305368e-51 | -8.615850e-47 |
| Msa0685360 | Msa0690440 | 0.828668 | 7.912513e-55 | -8.615850e-47 |
| Msa0685360 | Msa0698360 | 0.808770 | 2.630500e-50 | -8.615850e-47 |
| Msa0685360 | Msa0715470 | 0.800835 | 1.196192e-48 | -8.615850e-47 |
| Msa0685360 | Msa0725450 | 0.809663 | 1.692559e-50 | -8.615850e-47 |
| Msa0685360 | Msa0744170 | -0.803240 | 3.831020e-49 | -8.615850e-47 |
| Msa0685360 | Msa0766630 | 0.802712 | 4.924878e-49 | -8.615850e-47 |
| Msa0685360 | Msa0946690 | -0.805017 | 1.635435e-49 | -8.615850e-47 |
| Msa0685360 | Msa1082500 | 0.801077 | 1.067550e-48 | -8.615850e-47 |
| Msa0685360 | Msa1098420 | 0.800060 | 1.720506e-48 | -8.615850e-47 |
| Msa0685360 | Msa1102790 | 0.800767 | 1.235214e-48 | -8.615850e-47 |
| Msa0685360 | Msa1124540 | -0.806484 | 8.042235e-50 | -8.615850e-47 |
| Msa0685360 | Msa1224480 | 0.814182 | 1.754648e-51 | -8.615850e-47 |
| Msa0685360 | Msa1298260 | 0.810059 | 1.391183e-50 | -8.615850e-47 |
| Msa0685360 | Msa1305340 | 0.810712 | 1.005644e-50 | -8.615850e-47 |
| Msa0685360 | Msa1327550 | 0.802283 | 6.036960e-49 | -8.615850e-47 |
| Msa0685360 | Msa1401290 | 0.807837 | 4.158577e-50 | -8.615850e-47 |
| Msa0685360 | Msa1411020 | 0.809712 | 1.651959e-50 | -8.615850e-47 |
| Msa0685360 | Msa1420490 | 0.801945 | 7.084909e-49 | -8.615850e-47 |
| Msa0685360 | Msa1423390 | 0.802050 | 6.742932e-49 | -8.615850e-47 |
| Msa0685360 | Msa1424290 | 0.805078 | 1.588214e-49 | -8.615850e-47 |
| Msa0685360 | Msa1425100 | 0.800846 | 1.189924e-48 | -8.615850e-47 |
| Msa0685360 | Msa1434350 | 0.810122 | 1.348647e-50 | -8.615850e-47 |
| Msa0685360 | Msa1439320 | 0.811697 | 6.148529e-51 | -8.615850e-47 |
| Msa0685360 | Msa1440370 | 0.802785 | 4.756738e-49 | -8.615850e-47 |
| Msa0685360 | Msa1440810 | 0.807035 | 6.153122e-50 | -8.615850e-47 |
| Msa0685360 | Msa1444740 | 0.810100 | 1.363051e-50 | -8.615850e-47 |
| Msa0685360 | Msa1451620 | -0.807707 | 4.430924e-50 | -8.615850e-47 |
| Msa0685360 | Msa1462840 | 0.802096 | 6.598102e-49 | -8.615850e-47 |
| Msa0178860 | Msa0685360 | -0.830991 | 2.149571e-55 | -8.615850e-47 |
| Msa0204940 | Msa0685360 | 0.802195 | 6.295909e-49 | -8.615850e-47 |
| Msa0206010 | Msa0685360 | 0.807440 | 5.048644e-50 | -8.615850e-47 |
| Msa0217680 | Msa0685360 | 0.808352 | 3.230268e-50 | -8.615850e-47 |
| Msa0379640 | Msa0685360 | 0.820529 | 6.537785e-53 | -8.615850e-47 |
| Msa0412840 | Msa0685360 | 0.817493 | 3.204075e-52 | -8.615850e-47 |
| Msa0433410 | Msa0685360 | -0.811321 | 7.423265e-51 | -8.615850e-47 |
| Msa0442200 | Msa0685360 | 0.811930 | 5.470818e-51 | -8.615850e-47 |
| Msa0475490 | Msa0685360 | 0.804332 | 2.272807e-49 | -8.615850e-47 |
| Msa0477130 | Msa0685360 | -0.846219 | 2.491433e-59 | -8.615850e-47 |
| Msa0287410 | Msa0685360 | 0.803532 | 3.333159e-49 | -8.615850e-47 |
| Msa0306210 | Msa0685360 | -0.801005 | 1.104402e-48 | -8.615850e-47 |
| Msa0326110 | Msa0685360 | 0.805864 | 1.086704e-49 | -8.615850e-47 |
| Msa0493630 | Msa0685360 | 0.800955 | 1.130223e-48 | -8.615850e-47 |
| Msa0553460 | Msa0685360 | 0.814507 | 1.487542e-51 | -8.615850e-47 |
| Msa0575470 | Msa0685360 | 0.802704 | 4.945469e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0685360 | MtrunA17_Chr4g0033611 | 95.294 | 255 | 11 | 1 | 37 | 290 | 3 | 257 | 2.64e-173 | 479 |
| Msa0685360 | MtrunA17_Chr2g0329421 | 69.903 | 206 | 57 | 4 | 83 | 286 | 1 | 203 | 1.29e-99 | 291 |
| Msa0685360 | MtrunA17_Chr3g0078041 | 43.684 | 190 | 97 | 3 | 103 | 288 | 44 | 227 | 1.29e-43 | 148 |
| Msa0685360 | MtrunA17_Chr2g0329361 | 36.923 | 195 | 105 | 6 | 101 | 281 | 53 | 243 | 1.16e-34 | 125 |
| Msa0685360 | MtrunA17_Chr7g0233911 | 36.559 | 186 | 105 | 4 | 101 | 281 | 26 | 203 | 4.40e-33 | 120 |
| Msa0685360 | MtrunA17_Chr5g0448761 | 50.862 | 116 | 52 | 3 | 101 | 211 | 52 | 167 | 3.35e-22 | 96.7 |
| Msa0685360 | MtrunA17_Chr2g0287061 | 42.202 | 109 | 51 | 1 | 108 | 216 | 182 | 278 | 2.03e-21 | 94.4 |
| Msa0685360 | MtrunA17_Chr2g0287061 | 37.255 | 102 | 52 | 1 | 108 | 209 | 28 | 117 | 4.08e-16 | 78.6 |
| Msa0685360 | MtrunA17_Chr3g0082121 | 44.860 | 107 | 53 | 3 | 109 | 211 | 43 | 147 | 4.09e-20 | 90.5 |
| Msa0685360 | MtrunA17_Chr1g0203721 | 36.943 | 157 | 87 | 5 | 80 | 227 | 27 | 180 | 6.85e-20 | 89.7 |
| Msa0685360 | MtrunA17_Chr5g0401121 | 42.593 | 108 | 56 | 3 | 109 | 212 | 56 | 161 | 1.52e-19 | 83.6 |
| Msa0685360 | MtrunA17_Chr6g0458391 | 38.393 | 112 | 67 | 2 | 109 | 219 | 225 | 335 | 2.95e-19 | 87.8 |
| Msa0685360 | MtrunA17_Chr6g0458391 | 36.283 | 113 | 67 | 1 | 100 | 207 | 77 | 189 | 1.63e-15 | 76.6 |
| Msa0685360 | MtrunA17_Chr1g0202341 | 33.582 | 134 | 77 | 3 | 83 | 209 | 25 | 153 | 2.41e-18 | 85.1 |
| Msa0685360 | MtrunA17_Chr5g0440451 | 39.216 | 102 | 50 | 2 | 108 | 209 | 42 | 131 | 1.55e-15 | 77.0 |
| Msa0685360 | MtrunA17_Chr2g0291581 | 34.328 | 134 | 83 | 2 | 109 | 241 | 71 | 200 | 5.80e-15 | 75.1 |
| Msa0685360 | MtrunA17_Chr2g0291591 | 34.615 | 104 | 65 | 1 | 108 | 211 | 63 | 163 | 1.08e-14 | 73.9 |
| Msa0685360 | MtrunA17_Chr3g0080351 | 30.345 | 145 | 86 | 3 | 88 | 217 | 54 | 198 | 3.97e-14 | 72.8 |
| Msa0685360 | MtrunA17_Chr7g0258861 | 36.364 | 110 | 66 | 3 | 108 | 214 | 9 | 117 | 1.17e-12 | 67.0 |
| Msa0685360 | MtrunA17_Chr1g0207551 | 28.788 | 132 | 92 | 2 | 82 | 212 | 6 | 136 | 1.27e-11 | 65.1 |
| Msa0685360 | MtrunA17_Chr2g0329031 | 32.143 | 112 | 70 | 3 | 105 | 214 | 10 | 117 | 3.19e-11 | 63.9 |
| Msa0685360 | MtrunA17_Chr2g0276841 | 28.111 | 217 | 136 | 10 | 80 | 285 | 42 | 249 | 4.23e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0685360 | AT3G59470.3 | 59.227 | 233 | 84 | 4 | 66 | 290 | 20 | 249 | 1.50e-94 | 280 |
| Msa0685360 | AT3G59470.1 | 59.227 | 233 | 84 | 4 | 66 | 290 | 20 | 249 | 1.50e-94 | 280 |
| Msa0685360 | AT3G59470.2 | 59.227 | 233 | 84 | 4 | 66 | 290 | 32 | 261 | 2.39e-94 | 280 |
| Msa0685360 | AT3G07500.1 | 42.938 | 177 | 98 | 3 | 108 | 281 | 32 | 208 | 1.09e-43 | 149 |
| Msa0685360 | AT2G43280.1 | 38.571 | 210 | 105 | 5 | 77 | 281 | 2 | 192 | 4.89e-37 | 131 |
| Msa0685360 | AT4G12850.1 | 35.714 | 182 | 109 | 3 | 103 | 283 | 5 | 179 | 1.28e-31 | 116 |
| Msa0685360 | AT2G43280.2 | 39.877 | 163 | 86 | 4 | 124 | 281 | 2 | 157 | 1.26e-30 | 113 |
| Msa0685360 | AT4G38180.1 | 54.630 | 108 | 45 | 2 | 108 | 211 | 72 | 179 | 2.91e-24 | 102 |
| Msa0685360 | AT4G12850.2 | 40.650 | 123 | 70 | 2 | 103 | 224 | 5 | 125 | 4.35e-24 | 95.1 |
| Msa0685360 | AT4G12850.4 | 42.056 | 107 | 60 | 1 | 103 | 209 | 5 | 109 | 1.21e-23 | 94.4 |
| Msa0685360 | AT5G18960.2 | 36.184 | 152 | 71 | 2 | 66 | 217 | 14 | 139 | 9.85e-23 | 98.6 |
| Msa0685360 | AT5G18960.2 | 37.963 | 108 | 55 | 1 | 105 | 212 | 207 | 302 | 1.21e-19 | 89.4 |
| Msa0685360 | AT5G18960.1 | 36.184 | 152 | 71 | 2 | 66 | 217 | 14 | 139 | 9.85e-23 | 98.6 |
| Msa0685360 | AT5G18960.1 | 37.963 | 108 | 55 | 1 | 105 | 212 | 207 | 302 | 1.21e-19 | 89.4 |
| Msa0685360 | AT3G06250.2 | 38.793 | 116 | 59 | 1 | 106 | 221 | 187 | 290 | 8.14e-21 | 92.8 |
| Msa0685360 | AT3G06250.2 | 40.566 | 106 | 51 | 1 | 105 | 210 | 24 | 117 | 2.81e-20 | 91.3 |
| Msa0685360 | AT3G06250.1 | 38.793 | 116 | 59 | 1 | 106 | 221 | 187 | 290 | 8.14e-21 | 92.8 |
| Msa0685360 | AT3G06250.1 | 40.566 | 106 | 51 | 1 | 105 | 210 | 24 | 117 | 2.81e-20 | 91.3 |
| Msa0685360 | AT3G06250.3 | 38.793 | 116 | 59 | 1 | 106 | 221 | 187 | 290 | 8.14e-21 | 92.8 |
| Msa0685360 | AT3G06250.3 | 40.566 | 106 | 51 | 1 | 105 | 210 | 24 | 117 | 2.81e-20 | 91.3 |
| Msa0685360 | AT1G52520.1 | 39.604 | 101 | 58 | 1 | 109 | 209 | 85 | 182 | 2.73e-17 | 82.4 |
| Msa0685360 | AT1G80010.1 | 37.736 | 106 | 63 | 1 | 106 | 211 | 92 | 194 | 3.17e-16 | 79.3 |
| Msa0685360 | AT2G32250.2 | 38.182 | 110 | 62 | 2 | 104 | 211 | 33 | 138 | 5.49e-15 | 75.5 |
| Msa0685360 | AT2G32250.4 | 38.182 | 110 | 62 | 2 | 104 | 211 | 33 | 138 | 5.49e-15 | 75.5 |
| Msa0685360 | AT2G32250.5 | 38.182 | 110 | 62 | 2 | 104 | 211 | 33 | 138 | 5.54e-15 | 75.5 |
| Msa0685360 | AT2G32250.6 | 38.182 | 110 | 62 | 2 | 104 | 211 | 33 | 138 | 5.59e-15 | 75.5 |
| Msa0685360 | AT2G32250.1 | 38.182 | 110 | 62 | 2 | 104 | 211 | 33 | 138 | 5.59e-15 | 75.5 |
| Msa0685360 | AT2G32250.3 | 38.182 | 110 | 62 | 2 | 104 | 211 | 33 | 138 | 5.59e-15 | 75.5 |
| Msa0685360 | AT4G19990.3 | 28.966 | 145 | 75 | 2 | 112 | 242 | 24 | 154 | 2.84e-14 | 73.6 |
| Msa0685360 | AT4G19990.4 | 28.966 | 145 | 75 | 2 | 112 | 242 | 24 | 154 | 2.84e-14 | 73.6 |
| Msa0685360 | AT4G19990.7 | 28.966 | 145 | 75 | 2 | 112 | 242 | 38 | 168 | 2.86e-14 | 73.6 |
| Msa0685360 | AT4G19990.6 | 28.966 | 145 | 75 | 2 | 112 | 242 | 24 | 154 | 3.09e-14 | 73.2 |
| Msa0685360 | AT4G19990.2 | 28.966 | 145 | 75 | 2 | 112 | 242 | 24 | 154 | 3.09e-14 | 73.2 |
| Msa0685360 | AT4G19990.5 | 28.966 | 145 | 75 | 2 | 112 | 242 | 24 | 154 | 3.26e-14 | 73.2 |
| Msa0685360 | AT2G32250.7 | 39.000 | 100 | 55 | 2 | 114 | 211 | 2 | 97 | 5.56e-14 | 72.4 |
| Msa0685360 | AT2G32250.8 | 39.000 | 100 | 55 | 2 | 114 | 211 | 2 | 97 | 5.61e-14 | 72.4 |
| Msa0685360 | AT3G22170.2 | 28.859 | 149 | 89 | 4 | 84 | 217 | 46 | 192 | 1.43e-11 | 65.5 |
| Msa0685360 | AT3G22170.1 | 28.859 | 149 | 89 | 4 | 84 | 217 | 46 | 192 | 1.43e-11 | 65.5 |
Find 77 sgRNAs with CRISPR-Local
Find 109 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAAATTAATTCTGGTAAA+TGG | 0.122359 | 4_4:-49479864 | Msa0685360:CDS |
| TTATGTTCTTCTTCTTCTTT+AGG | 0.197944 | 4_4:+49478953 | None:intergenic |
| TCTGTATCGTTCTATGTATC+TGG | 0.244963 | 4_4:-49480436 | Msa0685360:CDS |
| CAGAAACAAGGGTTGGTTGC+AGG | 0.300475 | 4_4:-49479903 | Msa0685360:CDS |
| TCTATGTATCTGGGCTTCTT+AGG | 0.304144 | 4_4:-49480426 | Msa0685360:intron |
| CAATTGTGATAACTCATAAA+AGG | 0.333301 | 4_4:-49478664 | Msa0685360:three_prime_UTR |
| GAAAATTAATTCTGGTAAAT+GGG | 0.333770 | 4_4:-49479863 | Msa0685360:CDS |
| ATGATGAGGAAAATTAATTC+TGG | 0.337234 | 4_4:-49479871 | Msa0685360:CDS |
| ATCTCCTTTCTACTACTTCC+AGG | 0.346138 | 4_4:+49479799 | None:intergenic |
| GAGATTGATGACTCTGTTAA+CGG | 0.380618 | 4_4:-49480312 | Msa0685360:CDS |
| AGGAAAGACGGAACTGCTAT+TGG | 0.388968 | 4_4:-49479997 | Msa0685360:CDS |
| AACGTGACTCAAGATTCTTC+TGG | 0.391633 | 4_4:-49480186 | Msa0685360:CDS |
| CTTGAGTCACGTTATCATCT+TGG | 0.395456 | 4_4:+49480196 | None:intergenic |
| TCTTCTTCCATGTATTCATC+AGG | 0.412222 | 4_4:+49480246 | None:intergenic |
| TCTTGGTCTTGTTTGTAAGA+TGG | 0.422563 | 4_4:+49480213 | None:intergenic |
| TCTACTACTTCCAGGATTCA+AGG | 0.432111 | 4_4:+49479807 | None:intergenic |
| TATTATTGCACACCTCATCT+TGG | 0.432815 | 4_4:+49478852 | None:intergenic |
| AGAAGAACATAATCATTGAT+AGG | 0.432866 | 4_4:-49478942 | Msa0685360:exon |
| GGAGATGTTTGAAATTTATC+CGG | 0.433714 | 4_4:-49479782 | Msa0685360:intron |
| TTGTTAACGCATCTTGGTCA+TGG | 0.435422 | 4_4:-49480492 | Msa0685360:CDS |
| TTCTGCTGAGGAGCCTTATG+TGG | 0.437415 | 4_4:-49480118 | Msa0685360:CDS |
| ATATGTTTGTTAACGCATCT+TGG | 0.441858 | 4_4:-49480498 | Msa0685360:CDS |
| GGCTCCTCAGCAGAAGCGTC+TGG | 0.448272 | 4_4:+49480126 | None:intergenic |
| TGAGGAGCCTTATGTGGGTC+AGG | 0.449928 | 4_4:-49480112 | Msa0685360:CDS |
| GGAATAATTAAGCGCTATCA+AGG | 0.466188 | 4_4:+49478631 | None:intergenic |
| ACATAGAACGATACAGATAT+AGG | 0.468123 | 4_4:+49480442 | None:intergenic |
| CCAACTGCTGAGATAGTTCA+CGG | 0.468891 | 4_4:+49479104 | None:intergenic |
| TAAGCAAGCTCTCACGATCA+AGG | 0.471737 | 4_4:-49480017 | Msa0685360:CDS |
| CACACACATCCCTTGAATCC+TGG | 0.472295 | 4_4:-49479817 | Msa0685360:CDS |
| TGAGTCTATTCATATGAAGT+TGG | 0.472796 | 4_4:-49478693 | Msa0685360:three_prime_UTR |
| TGTTAACGCATCTTGGTCAT+GGG | 0.486848 | 4_4:-49480491 | Msa0685360:CDS |
| CTGTATCGTTCTATGTATCT+GGG | 0.499393 | 4_4:-49480435 | Msa0685360:CDS |
| TCGAACTCCTGACCCACATA+AGG | 0.503181 | 4_4:+49480105 | None:intergenic |
| CTACTACTTCCAGGATTCAA+GGG | 0.508347 | 4_4:+49479808 | None:intergenic |
| AGAAACAAGGGTTGGTTGCA+GGG | 0.508414 | 4_4:-49479902 | Msa0685360:CDS |
| AAATGTTGCAAAATCAGCTA+TGG | 0.513100 | 4_4:+49478610 | None:intergenic |
| GGTTATTACGAAGTTTGTAA+AGG | 0.521568 | 4_4:-49479842 | Msa0685360:CDS |
| AGCAGAAGCGTCTGGTAGTG+TGG | 0.524624 | 4_4:+49480134 | None:intergenic |
| AATGCATACGCAGCCGAGGT+TGG | 0.541461 | 4_4:-49480054 | Msa0685360:CDS |
| CCGTGAACTATCTCAGCAGT+TGG | 0.542386 | 4_4:-49479104 | Msa0685360:CDS |
| CGAAAAGATTGTGAGGCAAA+GGG | 0.544339 | 4_4:-49479926 | Msa0685360:CDS |
| GCGAAAAGATTGTGAGGCAA+AGG | 0.552809 | 4_4:-49479927 | Msa0685360:CDS |
| AGCATAATGTGGGTCTCTCA+AGG | 0.559262 | 4_4:-49479015 | Msa0685360:CDS |
| GGCTCTTGTTTGCAACAAAG+AGG | 0.560233 | 4_4:-49479971 | Msa0685360:CDS |
| TATCTAATAACTGCTGACAT+AGG | 0.563093 | 4_4:-49478831 | Msa0685360:three_prime_UTR |
| AGTAGACAGTGTGAAGGAGA+TGG | 0.569154 | 4_4:-49478978 | Msa0685360:CDS |
| GAATCCTGGAAGTAGTAGAA+AGG | 0.569556 | 4_4:-49479803 | Msa0685360:CDS |
| GATGGTGCCATGGTTGTAGC+TGG | 0.569629 | 4_4:-49480276 | Msa0685360:CDS |
| TGGTAGTGTGGGAACTGCAG+TGG | 0.577611 | 4_4:+49480146 | None:intergenic |
| GCTCTTGTTTGCAACAAAGA+GGG | 0.580043 | 4_4:-49479970 | Msa0685360:CDS |
| TGAGGCAAAGGGCAGAAACA+AGG | 0.583136 | 4_4:-49479915 | Msa0685360:CDS |
| TAGCATACCTGATGAATACA+TGG | 0.584054 | 4_4:-49480253 | Msa0685360:CDS |
| GCAGAAGCGTCTGGTAGTGT+GGG | 0.584434 | 4_4:+49480135 | None:intergenic |
| GGTAGTGTGGGAACTGCAGT+GGG | 0.584997 | 4_4:+49480147 | None:intergenic |
| AGACGGAACTGCTATTGGAC+GGG | 0.589838 | 4_4:-49479992 | Msa0685360:CDS |
| CTCTCACGATCAAGGAAAGA+CGG | 0.590017 | 4_4:-49480009 | Msa0685360:CDS |
| TTAGTATTAATATGATACAA+TGG | 0.596314 | 4_4:-49478799 | Msa0685360:three_prime_UTR |
| TAACGCATCTTGGTCATGGG+CGG | 0.612194 | 4_4:-49480488 | Msa0685360:CDS |
| AAGACGGAACTGCTATTGGA+CGG | 0.612304 | 4_4:-49479993 | Msa0685360:CDS |
| CTTCATATGAATAGACTCAA+AGG | 0.619140 | 4_4:+49478697 | None:intergenic |
| TCTGCTGAGGAGCCTTATGT+GGG | 0.621782 | 4_4:-49480117 | Msa0685360:CDS |
| AGTGATATTTGATTATATCG+AGG | 0.622955 | 4_4:-49479038 | Msa0685360:CDS |
| CAAAGGGCAGAAACAAGGGT+TGG | 0.628129 | 4_4:-49479910 | Msa0685360:CDS |
| TTACAATGCATACGCAGCCG+AGG | 0.629238 | 4_4:-49480058 | Msa0685360:CDS |
| TATGCTACCAGCTACAACCA+TGG | 0.631572 | 4_4:+49480269 | None:intergenic |
| GCGTATAGTAGACAGTGTGA+AGG | 0.633138 | 4_4:-49478984 | Msa0685360:CDS |
| TACAGTAAGAGATGGTGCCA+TGG | 0.635293 | 4_4:-49480286 | Msa0685360:CDS |
| TTATATCGAGGAGCATAATG+TGG | 0.635828 | 4_4:-49479026 | Msa0685360:CDS |
| TATATCGAGGAGCATAATGT+GGG | 0.635920 | 4_4:-49479025 | Msa0685360:CDS |
| ACTACCAGACGCTTCTGCTG+AGG | 0.646967 | 4_4:-49480130 | Msa0685360:CDS |
| AACGGCGATACAGTAAGAGA+TGG | 0.652494 | 4_4:-49480294 | Msa0685360:CDS |
| GAGGCAAAGGGCAGAAACAA+GGG | 0.664007 | 4_4:-49479914 | Msa0685360:CDS |
| GCAGGGCAATGATTATGATG+AGG | 0.664420 | 4_4:-49479885 | Msa0685360:CDS |
| ACAAGCGCGAAAAGATTGTG+AGG | 0.665448 | 4_4:-49479933 | Msa0685360:CDS |
| GTGACTCAAGATTCTTCTGG+AGG | 0.668755 | 4_4:-49480183 | Msa0685360:CDS |
| TGTGGGTCAGGAGTTCGAGT+CGG | 0.669514 | 4_4:-49480100 | Msa0685360:CDS |
| GCGTATGATGAATCCAACCT+CGG | 0.694329 | 4_4:+49480041 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATAATTTATACAATTCTT+CGG | + | chr4_4:49479379-49479398 | None:intergenic | 10.0% |
| !! | AAATGAAAATTAAAAATGAA+TGG | + | chr4_4:49479632-49479651 | None:intergenic | 10.0% |
| !! | TAAAAAAGTAGATAATTGAA+AGG | + | chr4_4:49480201-49480220 | None:intergenic | 15.0% |
| !! | TTAGTATTAATATGATACAA+TGG | - | chr4_4:49480314-49480333 | Msa0685360:CDS | 15.0% |
| !! | GAAAATTAATTCTGGTAAAT+GGG | - | chr4_4:49479250-49479269 | Msa0685360:intron | 20.0% |
| !!! | TGAAGTTTTTAGGATAATAT+TGG | - | chr4_4:49479537-49479556 | Msa0685360:intron | 20.0% |
| !!! | AATGGATGAAGTATTATATA+AGG | + | chr4_4:49479614-49479633 | None:intergenic | 20.0% |
| !!! | TAAGTAGTTAAAGGTATTAT+AGG | - | chr4_4:49479773-49479792 | Msa0685360:intron | 20.0% |
| !!! | TTCTTGTTATGTAGTTATAT+TGG | - | chr4_4:49479807-49479826 | Msa0685360:CDS | 20.0% |
| ! | ATGATGAGGAAAATTAATTC+TGG | - | chr4_4:49479242-49479261 | Msa0685360:intron | 25.0% |
| ! | GGAAAATTAATTCTGGTAAA+TGG | - | chr4_4:49479249-49479268 | Msa0685360:intron | 25.0% |
| ! | GATTCTCGTATATATTGTAA+TGG | - | chr4_4:49479425-49479444 | Msa0685360:intron | 25.0% |
| !! | GAATGAATGGTATTAGTTTA+AGG | - | chr4_4:49479487-49479506 | Msa0685360:intron | 25.0% |
| !! | CTAAAAACTTCATTCTGAAA+TGG | + | chr4_4:49479529-49479548 | None:intergenic | 25.0% |
| !! | CATTTCAGAATGAAGTTTTT+AGG | - | chr4_4:49479527-49479546 | Msa0685360:intron | 25.0% |
| ! | ATGAGATGATAAGTAGTTAA+AGG | - | chr4_4:49479764-49479783 | Msa0685360:intron | 25.0% |
| ! | CAACTTAGAAATTACCTTTA+CGG | - | chr4_4:49479859-49479878 | Msa0685360:CDS | 25.0% |
| ! | AGTGATATTTGATTATATCG+AGG | - | chr4_4:49480075-49480094 | Msa0685360:CDS | 25.0% |
| ! | TTATGTTCTTCTTCTTCTTT+AGG | + | chr4_4:49480163-49480182 | None:intergenic | 25.0% |
| ! | AGAAGAACATAATCATTGAT+AGG | - | chr4_4:49480171-49480190 | Msa0685360:CDS | 25.0% |
| !! | CTATTTTCAATCGAATGTAT+AGG | - | chr4_4:49480392-49480411 | Msa0685360:intron | 25.0% |
| ! | CAATTGTGATAACTCATAAA+AGG | - | chr4_4:49480449-49480468 | Msa0685360:CDS | 25.0% |
| ACATAGAACGATACAGATAT+AGG | + | chr4_4:49478674-49478693 | None:intergenic | 30.0% | |
| ! | CTATAAGATTTTCAGTGTTC+AGG | + | chr4_4:49478753-49478772 | None:intergenic | 30.0% |
| GGTTATTACGAAGTTTGTAA+AGG | - | chr4_4:49479271-49479290 | Msa0685360:intron | 30.0% | |
| ! | GGAGATGTTTGAAATTTATC+CGG | - | chr4_4:49479331-49479350 | Msa0685360:intron | 30.0% |
| AAGCATAAAGTAACAATGAC+AGG | + | chr4_4:49479457-49479476 | None:intergenic | 30.0% | |
| CTTGATAGATTGAGAATGAA+TGG | - | chr4_4:49479474-49479493 | Msa0685360:intron | 30.0% | |
| AGTTAATTCTGCATTGAACT+TGG | + | chr4_4:49479689-49479708 | None:intergenic | 30.0% | |
| TTTATCGCAGAGAGATAAAT+AGG | - | chr4_4:49479737-49479756 | Msa0685360:intron | 30.0% | |
| TTAAAAAATGCTCCAAGATG+AGG | - | chr4_4:49480249-49480268 | Msa0685360:CDS | 30.0% | |
| TATCTAATAACTGCTGACAT+AGG | - | chr4_4:49480282-49480301 | Msa0685360:CDS | 30.0% | |
| CTTCATATGAATAGACTCAA+AGG | + | chr4_4:49480419-49480438 | None:intergenic | 30.0% | |
| TGAGTCTATTCATATGAAGT+TGG | - | chr4_4:49480420-49480439 | Msa0685360:intron | 30.0% | |
| TCTGTATCGTTCTATGTATC+TGG | - | chr4_4:49478677-49478696 | Msa0685360:three_prime_UTR | 35.0% | |
| CTGTATCGTTCTATGTATCT+GGG | - | chr4_4:49478678-49478697 | Msa0685360:three_prime_UTR | 35.0% | |
| ! | GAGATTGATGACTCTGTTAA+CGG | - | chr4_4:49478801-49478820 | Msa0685360:three_prime_UTR | 35.0% |
| TAGCATACCTGATGAATACA+TGG | - | chr4_4:49478860-49478879 | Msa0685360:three_prime_UTR | 35.0% | |
| TCTTCTTCCATGTATTCATC+AGG | + | chr4_4:49478870-49478889 | None:intergenic | 35.0% | |
| !! | TCTTGGTCTTGTTTGTAAGA+TGG | + | chr4_4:49478903-49478922 | None:intergenic | 35.0% |
| ! | TAAAAAGCATAGGAACTGAC+CGG | + | chr4_4:49479353-49479372 | None:intergenic | 35.0% |
| ! | TCTTCGGTAGTAAAAAGCAT+AGG | + | chr4_4:49479363-49479382 | None:intergenic | 35.0% |
| AGAAACAAACGAGGTAAGTA+GGG | + | chr4_4:49479959-49479978 | None:intergenic | 35.0% | |
| TAGAAACAAACGAGGTAAGT+AGG | + | chr4_4:49479960-49479979 | None:intergenic | 35.0% | |
| ! | TTATATCGAGGAGCATAATG+TGG | - | chr4_4:49480087-49480106 | Msa0685360:CDS | 35.0% |
| ! | TATATCGAGGAGCATAATGT+GGG | - | chr4_4:49480088-49480107 | Msa0685360:CDS | 35.0% |
| TATTATTGCACACCTCATCT+TGG | + | chr4_4:49480264-49480283 | None:intergenic | 35.0% | |
| GGAATAATTAAGCGCTATCA+AGG | + | chr4_4:49480485-49480504 | None:intergenic | 35.0% | |
| TTGTTAACGCATCTTGGTCA+TGG | - | chr4_4:49478621-49478640 | Msa0685360:three_prime_UTR | 40.0% | |
| TGTTAACGCATCTTGGTCAT+GGG | - | chr4_4:49478622-49478641 | Msa0685360:three_prime_UTR | 40.0% | |
| TCTATGTATCTGGGCTTCTT+AGG | - | chr4_4:49478687-49478706 | Msa0685360:three_prime_UTR | 40.0% | |
| !!! | CAGATCTTTTGTTGTTGCAG+TGG | - | chr4_4:49478773-49478792 | Msa0685360:three_prime_UTR | 40.0% |
| CTTGAGTCACGTTATCATCT+TGG | + | chr4_4:49478920-49478939 | None:intergenic | 40.0% | |
| AACGTGACTCAAGATTCTTC+TGG | - | chr4_4:49478927-49478946 | Msa0685360:exon | 40.0% | |
| ! | GCTCTTGTTTGCAACAAAGA+GGG | - | chr4_4:49479143-49479162 | Msa0685360:intron | 40.0% |
| CGAAAAGATTGTGAGGCAAA+GGG | - | chr4_4:49479187-49479206 | Msa0685360:intron | 40.0% | |
| CTACTACTTCCAGGATTCAA+GGG | + | chr4_4:49479308-49479327 | None:intergenic | 40.0% | |
| TCTACTACTTCCAGGATTCA+AGG | + | chr4_4:49479309-49479328 | None:intergenic | 40.0% | |
| GAATCCTGGAAGTAGTAGAA+AGG | - | chr4_4:49479310-49479329 | Msa0685360:intron | 40.0% | |
| ATCTCCTTTCTACTACTTCC+AGG | + | chr4_4:49479317-49479336 | None:intergenic | 40.0% | |
| TTTCTCTCGTGGAAAGGATA+AGG | + | chr4_4:49479836-49479855 | None:intergenic | 40.0% | |
| TTGCAATTTCTCTCGTGGAA+AGG | + | chr4_4:49479842-49479861 | None:intergenic | 40.0% | |
| CTAAGTTGCAATTTCTCTCG+TGG | + | chr4_4:49479847-49479866 | None:intergenic | 40.0% | |
| AAACAAACGAGGTAAGTAGG+GGG | + | chr4_4:49479957-49479976 | None:intergenic | 40.0% | |
| GAAACAAACGAGGTAAGTAG+GGG | + | chr4_4:49479958-49479977 | None:intergenic | 40.0% | |
| GACTGACATAGAAACAAACG+AGG | + | chr4_4:49479968-49479987 | None:intergenic | 40.0% | |
| AACGGCGATACAGTAAGAGA+TGG | - | chr4_4:49478819-49478838 | Msa0685360:three_prime_UTR | 45.0% | |
| ! | TACAGTAAGAGATGGTGCCA+TGG | - | chr4_4:49478827-49478846 | Msa0685360:three_prime_UTR | 45.0% |
| TATGCTACCAGCTACAACCA+TGG | + | chr4_4:49478847-49478866 | None:intergenic | 45.0% | |
| GTGACTCAAGATTCTTCTGG+AGG | - | chr4_4:49478930-49478949 | Msa0685360:exon | 45.0% | |
| GCGTATGATGAATCCAACCT+CGG | + | chr4_4:49479075-49479094 | None:intergenic | 45.0% | |
| TAAGCAAGCTCTCACGATCA+AGG | - | chr4_4:49479096-49479115 | Msa0685360:CDS | 45.0% | |
| CTCTCACGATCAAGGAAAGA+CGG | - | chr4_4:49479104-49479123 | Msa0685360:CDS | 45.0% | |
| AGGAAAGACGGAACTGCTAT+TGG | - | chr4_4:49479116-49479135 | Msa0685360:CDS | 45.0% | |
| AAGACGGAACTGCTATTGGA+CGG | - | chr4_4:49479120-49479139 | Msa0685360:CDS | 45.0% | |
| ! | GGCTCTTGTTTGCAACAAAG+AGG | - | chr4_4:49479142-49479161 | Msa0685360:intron | 45.0% |
| ! | ACAATCTTTTCGCGCTTGTC+AGG | + | chr4_4:49479179-49479198 | None:intergenic | 45.0% |
| ACAAGCGCGAAAAGATTGTG+AGG | - | chr4_4:49479180-49479199 | Msa0685360:intron | 45.0% | |
| GCGAAAAGATTGTGAGGCAA+AGG | - | chr4_4:49479186-49479205 | Msa0685360:intron | 45.0% | |
| !! | AGAAACAAGGGTTGGTTGCA+GGG | - | chr4_4:49479211-49479230 | Msa0685360:intron | 45.0% |
| GCAGGGCAATGATTATGATG+AGG | - | chr4_4:49479228-49479247 | Msa0685360:intron | 45.0% | |
| !! | ACATGCAGAAAGTGCCGTAA+AGG | + | chr4_4:49479876-49479895 | None:intergenic | 45.0% |
| CCAACTGCTGAGATAGTTCA+CGG | + | chr4_4:49480012-49480031 | None:intergenic | 45.0% | |
| AGCATAATGTGGGTCTCTCA+AGG | - | chr4_4:49480098-49480117 | Msa0685360:CDS | 45.0% | |
| GCGTATAGTAGACAGTGTGA+AGG | - | chr4_4:49480129-49480148 | Msa0685360:CDS | 45.0% | |
| AGTAGACAGTGTGAAGGAGA+TGG | - | chr4_4:49480135-49480154 | Msa0685360:CDS | 45.0% | |
| TAACGCATCTTGGTCATGGG+CGG | - | chr4_4:49478625-49478644 | Msa0685360:three_prime_UTR | 50.0% | |
| ! | TTCTGCTGAGGAGCCTTATG+TGG | - | chr4_4:49478995-49479014 | Msa0685360:CDS | 50.0% |
| ! | TCTGCTGAGGAGCCTTATGT+GGG | - | chr4_4:49478996-49479015 | Msa0685360:CDS | 50.0% |
| TCGAACTCCTGACCCACATA+AGG | + | chr4_4:49479011-49479030 | None:intergenic | 50.0% | |
| TTACAATGCATACGCAGCCG+AGG | - | chr4_4:49479055-49479074 | Msa0685360:CDS | 50.0% | |
| AGACGGAACTGCTATTGGAC+GGG | - | chr4_4:49479121-49479140 | Msa0685360:CDS | 50.0% | |
| TGAGGCAAAGGGCAGAAACA+AGG | - | chr4_4:49479198-49479217 | Msa0685360:intron | 50.0% | |
| GAGGCAAAGGGCAGAAACAA+GGG | - | chr4_4:49479199-49479218 | Msa0685360:intron | 50.0% | |
| CAAAGGGCAGAAACAAGGGT+TGG | - | chr4_4:49479203-49479222 | Msa0685360:intron | 50.0% | |
| !! | CAGAAACAAGGGTTGGTTGC+AGG | - | chr4_4:49479210-49479229 | Msa0685360:intron | 50.0% |
| CACACACATCCCTTGAATCC+TGG | - | chr4_4:49479296-49479315 | Msa0685360:intron | 50.0% | |
| CTCTCGTGGAAAGGATAAGG+TGG | + | chr4_4:49479833-49479852 | None:intergenic | 50.0% | |
| CCGTGAACTATCTCAGCAGT+TGG | - | chr4_4:49480009-49480028 | Msa0685360:CDS | 50.0% | |
| !! | GATGGTGCCATGGTTGTAGC+TGG | - | chr4_4:49478837-49478856 | Msa0685360:three_prime_UTR | 55.0% |
| GGTAGTGTGGGAACTGCAGT+GGG | + | chr4_4:49478969-49478988 | None:intergenic | 55.0% | |
| ! | TGGTAGTGTGGGAACTGCAG+TGG | + | chr4_4:49478970-49478989 | None:intergenic | 55.0% |
| !! | GCAGAAGCGTCTGGTAGTGT+GGG | + | chr4_4:49478981-49479000 | None:intergenic | 55.0% |
| !! | AGCAGAAGCGTCTGGTAGTG+TGG | + | chr4_4:49478982-49479001 | None:intergenic | 55.0% |
| ACTACCAGACGCTTCTGCTG+AGG | - | chr4_4:49478983-49479002 | Msa0685360:CDS | 55.0% | |
| ! | TGAGGAGCCTTATGTGGGTC+AGG | - | chr4_4:49479001-49479020 | Msa0685360:CDS | 55.0% |
| TGTGGGTCAGGAGTTCGAGT+CGG | - | chr4_4:49479013-49479032 | Msa0685360:CDS | 55.0% | |
| AATGCATACGCAGCCGAGGT+TGG | - | chr4_4:49479059-49479078 | Msa0685360:CDS | 55.0% | |
| !! | GGCTCCTCAGCAGAAGCGTC+TGG | + | chr4_4:49478990-49479009 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 49478617 | 49480518 | 49478617 | ID=Msa0685360;Name=Msa0685360 |
| chr4_4 | mRNA | 49478617 | 49480518 | 49478617 | ID=Msa0685360-mRNA-1;Parent=Msa0685360;Name=Msa0685360-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|0.5|0.33|1|1|1|3|329|290 |
| chr4_4 | exon | 49478617 | 49479149 | 49478617 | ID=Msa0685360-mRNA-1:exon:12457;Parent=Msa0685360-mRNA-1 |
| chr4_4 | exon | 49479783 | 49480359 | 49479783 | ID=Msa0685360-mRNA-1:exon:12456;Parent=Msa0685360-mRNA-1 |
| chr4_4 | exon | 49480427 | 49480518 | 49480427 | ID=Msa0685360-mRNA-1:exon:12455;Parent=Msa0685360-mRNA-1 |
| chr4_4 | CDS | 49480427 | 49480518 | 49480427 | ID=Msa0685360-mRNA-1:cds;Parent=Msa0685360-mRNA-1 |
| chr4_4 | CDS | 49479783 | 49480359 | 49479783 | ID=Msa0685360-mRNA-1:cds;Parent=Msa0685360-mRNA-1 |
| chr4_4 | CDS | 49478946 | 49479149 | 49478946 | ID=Msa0685360-mRNA-1:cds;Parent=Msa0685360-mRNA-1 |
| chr4_4 | three_prime_UTR | 49478617 | 49478945 | 49478617 | ID=Msa0685360-mRNA-1:three_prime_utr;Parent=Msa0685360-mRNA-1 |
| Gene Sequence |
| Protein sequence |
