AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0944930


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 40 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAAAAGAAAGTAGGTTTC+GGG 0.299621 6_3:-22377508 Msa0944930:intron
TGCGCGAGTCACCTTCTTCT+AGG 0.325482 6_3:-22377331 Msa0944930:CDS
ACCAGCCAGCTGGGTTGGTT+TGG 0.332153 6_3:-22377390 Msa0944930:CDS
CGCAATGGCTTTCTCTGATA+GGG 0.371132 6_3:-22377796 None:intergenic
ATTCCTTTCTATTGAAAAGA+AGG 0.387353 6_3:-22377365 Msa0944930:CDS
GCGCAATGGCTTTCTCTGAT+AGG 0.391358 6_3:-22377797 None:intergenic
GTCGTCCGTCTCAGGTTTGC+CGG 0.398406 6_3:-22377434 Msa0944930:CDS
GGACCGTGGTCGTCCGTCTC+AGG 0.441663 6_3:-22377442 Msa0944930:CDS
AGCAGCACCTTGTATAGGTT+GGG 0.447543 6_3:+22377753 None:intergenic
GTACACCTCCGGTGCTGATA+AGG 0.450222 6_3:-22377468 Msa0944930:CDS
GATTGACCAGCCAGCTGGGT+TGG 0.459599 6_3:-22377395 Msa0944930:CDS
GTCAATCTCAGAGATCTTAT+CGG 0.462954 6_3:+22377411 None:intergenic
AAGCGAGCAGCACCTTGTAT+AGG 0.473472 6_3:+22377748 None:intergenic
TCTGAGATTGACCAGCCAGC+TGG 0.486873 6_3:-22377400 Msa0944930:CDS
TTCATTGATTAGTACACCTC+CGG 0.492064 6_3:-22377479 Msa0944930:CDS
CGCTTTCTCTCAGCCACCAG+TGG 0.505851 6_3:-22377730 Msa0944930:CDS
CTGAGATTGACCAGCCAGCT+GGG 0.514570 6_3:-22377399 Msa0944930:CDS
CACCTTCTTCTAGGCTCCGC+TGG 0.516734 6_3:-22377322 Msa0944930:CDS
GCCAAACCAACCCAGCTGGC+TGG 0.523772 6_3:+22377389 None:intergenic
GAGCAGCACCTTGTATAGGT+TGG 0.534433 6_3:+22377752 None:intergenic
ATCTCAGAGATCTTATCGGC+CGG 0.550066 6_3:+22377415 None:intergenic
AATAGCCAAACCAACCCAGC+TGG 0.553661 6_3:+22377385 None:intergenic
CCTCCGGTGCTGATAAGGTC+GGG 0.561319 6_3:-22377463 Msa0944930:CDS
ATCGGCCGGCAAACCTGAGA+CGG 0.579939 6_3:+22377429 None:intergenic
GCCACCAGTGGCTTATGTAG+TGG 0.581920 6_3:-22377718 Msa0944930:intron
TGCTGATAAGGTCGGGACCG+TGG 0.590314 6_3:-22377456 Msa0944930:CDS
GTCCAGCGGAGCCTAGAAGA+AGG 0.604022 6_3:+22377320 None:intergenic
CTAGGCTCCGCTGGACGACA+CGG 0.612977 6_3:-22377313 Msa0944930:CDS
GAGAATGCCGTGTCGTCCAG+CGG 0.617479 6_3:+22377306 None:intergenic
CCCGACCTTATCAGCACCGG+AGG 0.624401 6_3:+22377463 None:intergenic
AGCACCTTGTATAGGTTGGG+CGG 0.628644 6_3:+22377756 None:intergenic
AGCTCCGCCCAACCTATACA+AGG 0.631063 6_3:-22377760 Msa0944930:CDS
CCGACCACTACATAAGCCAC+TGG 0.631161 6_3:+22377714 None:intergenic
ACCTCCGGTGCTGATAAGGT+CGG 0.637828 6_3:-22377464 Msa0944930:CDS
GCAATGGCTTTCTCTGATAG+GGG 0.641776 6_3:-22377795 Msa0944930:CDS
GGTCCCGACCTTATCAGCAC+CGG 0.654281 6_3:+22377460 None:intergenic
AAACCTGAGACGGACGACCA+CGG 0.659367 6_3:+22377439 None:intergenic
ACCACTACATAAGCCACTGG+TGG 0.667213 6_3:+22377717 None:intergenic
AGAAAAGAAAGTAGGTTTCG+GGG 0.671452 6_3:-22377507 Msa0944930:intron
GAAAAGAAAGTAGGTTTCGG+GGG 0.676234 6_3:-22377506 Msa0944930:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTTGTCAGAAAAGAAAGT+AGG - chr6_3:22377577-22377596 Msa0944930:intron 25.0%
! ATTCCTTTCTATTGAAAAGA+AGG - chr6_3:22377727-22377746 Msa0944930:CDS 25.0%
TCAGAAAAGAAAGTAGGTTT+CGG - chr6_3:22377583-22377602 Msa0944930:intron 30.0%
! ACTCCTTCTTTTCAATAGAA+AGG + chr6_3:22377733-22377752 None:intergenic 30.0%
ACCTTTGGATTCTTAAGTAG+GGG + chr6_3:22377427-22377446 None:intergenic 35.0%
GACCTTTGGATTCTTAAGTA+GGG + chr6_3:22377428-22377447 None:intergenic 35.0%
TGACCTTTGGATTCTTAAGT+AGG + chr6_3:22377429-22377448 None:intergenic 35.0%
GTTGTTGTGATTGAGTTTGA+GGG + chr6_3:22377531-22377550 None:intergenic 35.0%
TGTTGTTGTGATTGAGTTTG+AGG + chr6_3:22377532-22377551 None:intergenic 35.0%
CAGAAAAGAAAGTAGGTTTC+GGG - chr6_3:22377584-22377603 Msa0944930:intron 35.0%
AGAAAAGAAAGTAGGTTTCG+GGG - chr6_3:22377585-22377604 Msa0944930:intron 35.0%
! TTCATTGATTAGTACACCTC+CGG - chr6_3:22377613-22377632 Msa0944930:intron 35.0%
GTCAATCTCAGAGATCTTAT+CGG + chr6_3:22377684-22377703 None:intergenic 35.0%
CCTTTGGATTCTTAAGTAGG+GGG + chr6_3:22377426-22377445 None:intergenic 40.0%
AAGAATCCAAAGGTCACCTT+TGG - chr6_3:22377433-22377452 Msa0944930:CDS 40.0%
GAAAAGAAAGTAGGTTTCGG+GGG - chr6_3:22377586-22377605 Msa0944930:intron 40.0%
AGCAGCACCTTGTATAGGTT+GGG + chr6_3:22377342-22377361 None:intergenic 45.0%
TTCTTAAGTAGGGGGCAAAG+AGG + chr6_3:22377418-22377437 None:intergenic 45.0%
CCCCCTACTTAAGAATCCAA+AGG - chr6_3:22377423-22377442 Msa0944930:CDS 45.0%
GCTTTGGTTACGAAAGTAGC+CGG - chr6_3:22377469-22377488 Msa0944930:CDS 45.0%
CTTTGGTTACGAAAGTAGCC+GGG - chr6_3:22377470-22377489 Msa0944930:CDS 45.0%
TTTGGTTACGAAAGTAGCCG+GGG - chr6_3:22377471-22377490 Msa0944930:CDS 45.0%
TCACAACAACATGTCGTGAC+CGG - chr6_3:22377541-22377560 Msa0944930:intron 45.0%
CAAAATCCACCAAGCCTAAC+CGG + chr6_3:22377563-22377582 None:intergenic 45.0%
ATCTCAGAGATCTTATCGGC+CGG + chr6_3:22377680-22377699 None:intergenic 45.0%
! AGCACCTTGTATAGGTTGGG+CGG + chr6_3:22377339-22377358 None:intergenic 50.0%
GAGCAGCACCTTGTATAGGT+TGG + chr6_3:22377343-22377362 None:intergenic 50.0%
AAGCGAGCAGCACCTTGTAT+AGG + chr6_3:22377347-22377366 None:intergenic 50.0%
ACCACTACATAAGCCACTGG+TGG + chr6_3:22377378-22377397 None:intergenic 50.0%
CCAGTGGCTTATGTAGTGGT+CGG - chr6_3:22377378-22377397 Msa0944930:CDS 50.0%
CAACAGCCAAAGGTGACCTT+TGG + chr6_3:22377442-22377461 None:intergenic 50.0%
AGCGTCAAGACAACAGCCAA+AGG + chr6_3:22377452-22377471 None:intergenic 50.0%
TGGCTGTTGTCTTGACGCTT+TGG - chr6_3:22377453-22377472 Msa0944930:CDS 50.0%
CCATCCATAAACCTAGACCC+CGG + chr6_3:22377491-22377510 None:intergenic 50.0%
ACAACATGTCGTGACCGGTT+AGG - chr6_3:22377546-22377565 Msa0944930:intron 50.0%
AAGAAAGTAGGTTTCGGGGG+TGG - chr6_3:22377589-22377608 Msa0944930:intron 50.0%
AGAAAGTAGGTTTCGGGGGT+GGG - chr6_3:22377590-22377609 Msa0944930:intron 50.0%
AATAGCCAAACCAACCCAGC+TGG + chr6_3:22377710-22377729 None:intergenic 50.0%
AGCTCCGCCCAACCTATACA+AGG - chr6_3:22377332-22377351 Msa0944930:CDS 55.0%
GCCACCAGTGGCTTATGTAG+TGG - chr6_3:22377374-22377393 Msa0944930:CDS 55.0%
CCGACCACTACATAAGCCAC+TGG + chr6_3:22377381-22377400 None:intergenic 55.0%
TACGAAAGTAGCCGGGGTCT+AGG - chr6_3:22377477-22377496 Msa0944930:CDS 55.0%
GTAGCCGGGGTCTAGGTTTA+TGG - chr6_3:22377484-22377503 Msa0944930:CDS 55.0%
CCGGGGTCTAGGTTTATGGA+TGG - chr6_3:22377488-22377507 Msa0944930:CDS 55.0%
ATGTCGTGACCGGTTAGGCT+TGG - chr6_3:22377551-22377570 Msa0944930:intron 55.0%
!! GTACACCTCCGGTGCTGATA+AGG - chr6_3:22377624-22377643 Msa0944930:intron 55.0%
!! ACCTCCGGTGCTGATAAGGT+CGG - chr6_3:22377628-22377647 Msa0944930:intron 55.0%
AAACCTGAGACGGACGACCA+CGG + chr6_3:22377656-22377675 None:intergenic 55.0%
! TCTGAGATTGACCAGCCAGC+TGG - chr6_3:22377692-22377711 Msa0944930:intron 55.0%
CTGAGATTGACCAGCCAGCT+GGG - chr6_3:22377693-22377712 Msa0944930:intron 55.0%
TGCGCGAGTCACCTTCTTCT+AGG - chr6_3:22377761-22377780 Msa0944930:CDS 55.0%
CGCTTTCTCTCAGCCACCAG+TGG - chr6_3:22377362-22377381 Msa0944930:CDS 60.0%
TCGTGACCGGTTAGGCTTGG+TGG - chr6_3:22377554-22377573 Msa0944930:intron 60.0%
!! CCTCCGGTGCTGATAAGGTC+GGG - chr6_3:22377629-22377648 Msa0944930:intron 60.0%
GGTCCCGACCTTATCAGCAC+CGG + chr6_3:22377635-22377654 None:intergenic 60.0%
TGCTGATAAGGTCGGGACCG+TGG - chr6_3:22377636-22377655 Msa0944930:intron 60.0%
GTCGTCCGTCTCAGGTTTGC+CGG - chr6_3:22377658-22377677 Msa0944930:intron 60.0%
ATCGGCCGGCAAACCTGAGA+CGG + chr6_3:22377666-22377685 None:intergenic 60.0%
GATTGACCAGCCAGCTGGGT+TGG - chr6_3:22377697-22377716 Msa0944930:intron 60.0%
!! ACCAGCCAGCTGGGTTGGTT+TGG - chr6_3:22377702-22377721 Msa0944930:intron 60.0%
CACCTTCTTCTAGGCTCCGC+TGG - chr6_3:22377770-22377789 Msa0944930:CDS 60.0%
GTCCAGCGGAGCCTAGAAGA+AGG + chr6_3:22377775-22377794 None:intergenic 60.0%
GAGAATGCCGTGTCGTCCAG+CGG + chr6_3:22377789-22377808 None:intergenic 60.0%
! GGGGGCAAAGAGGAGCACGT+AGG + chr6_3:22377408-22377427 None:intergenic 65.0%
! CCCGACCTTATCAGCACCGG+AGG + chr6_3:22377632-22377651 None:intergenic 65.0%
GCCAAACCAACCCAGCTGGC+TGG + chr6_3:22377706-22377725 None:intergenic 65.0%
CTAGGCTCCGCTGGACGACA+CGG - chr6_3:22377779-22377798 Msa0944930:CDS 65.0%
GGACCGTGGTCGTCCGTCTC+AGG - chr6_3:22377650-22377669 Msa0944930:intron 70.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 22377300 22377814 22377300 ID=Msa0944930;Name=Msa0944930
chr6_3 mRNA 22377300 22377814 22377300 ID=Msa0944930-mRNA-1;Parent=Msa0944930;Name=Msa0944930-mRNA-1;_AED=0.27;_eAED=0.30;_QI=0|0|0|1|0|0|2|0|103
chr6_3 exon 22377719 22377814 22377719 ID=Msa0944930-mRNA-1:exon:6577;Parent=Msa0944930-mRNA-1
chr6_3 exon 22377300 22377515 22377300 ID=Msa0944930-mRNA-1:exon:6576;Parent=Msa0944930-mRNA-1
chr6_3 CDS 22377719 22377814 22377719 ID=Msa0944930-mRNA-1:cds;Parent=Msa0944930-mRNA-1
chr6_3 CDS 22377300 22377515 22377300 ID=Msa0944930-mRNA-1:cds;Parent=Msa0944930-mRNA-1
Gene Sequence

>Msa0944930

ATGGCTTTCTCTGATAGGGGCTCGCTTCTTCAAGCTCCGCCCAACCTATACAAGGTGCTGCTCGCTTTCTCTCAGCCACCAGTGGCTTATGTAGTGGTTTCGGGGGTGGGTTCATTGATTAGTACACCTCCGGTGCTGATAAGGTCGGGACCGTGGTCGTCCGTCTCAGGTTTGCCGGCCGATAAGATCTCTGAGATTGACCAGCCAGCTGGGTTGGTTTGGCTATTCCTTTCTATTGAAAAGAAGGAGTCAGCTGTCTGCGCGAGTCACCTTCTTCTAGGCTCCGCTGGACGACACGGCATTCTCGTTTAA

Protein sequence

>Msa0944930

MAFSDRGSLLQAPPNLYKVLLAFSQPPVAYVVVSGVGSLISTPPVLIRSGPWSSVSGLPADKISEIDQPAGLVWLFLSIEKKESAVCASHLLLGSAGRHGILV*