AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0983260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGTCTGCTATGCGTTTA+AGG 0.136313 6_4:-26408067 None:intergenic
TCGTCTTGTACCTGTCCTTT+TGG 0.241491 6_4:-26407500 None:intergenic
CGTTAAGCCGCGAGAGTTAC+AGG 0.278769 6_4:+26407947 Msa0983260:CDS
AGCATTCTTTGTGGCCCTAT+TGG 0.280009 6_4:+26407391 None:intergenic
TTGGTTGTCACTCGAGTTAC+AGG 0.291580 6_4:+26408008 Msa0983260:CDS
CTGTACGTTCCTTTAGCTAT+AGG 0.323026 6_4:+26408227 Msa0983260:CDS
CGGATATGCTAGTCAAGATT+TGG 0.353308 6_4:+26407989 Msa0983260:CDS
AAAGTCTCCTTTCTTCTTGA+CGG 0.366612 6_4:+26408140 Msa0983260:CDS
TGTTCTTAAAGCATTCTTTG+TGG 0.408726 6_4:+26407382 None:intergenic
TGGCCCTATTGGATCTAGTA+AGG 0.416151 6_4:+26407402 Msa0983260:five_prime_UTR
TTCCCCTTACTAGATCCAAT+AGG 0.442615 6_4:-26407406 None:intergenic
TCAGCATGAAAAGGCATTGA+AGG 0.461639 6_4:-26407550 None:intergenic
GCTGTCTGCTATGCGTTTAA+GGG 0.465623 6_4:-26408066 None:intergenic
CTCTAAACCGTCAAGAAGAA+AGG 0.482951 6_4:-26408147 None:intergenic
TATGTGGAACGGACTATTCT+CGG 0.483217 6_4:-26408260 None:intergenic
TCCCCTTACTAGATCCAATA+GGG 0.483736 6_4:-26407405 None:intergenic
AGTAAGGGGAAGGCGCATAA+TGG 0.485728 6_4:+26407418 Msa0983260:five_prime_UTR
AAGAGAGAGGCTGTCCCAAA+AGG 0.504987 6_4:+26407485 Msa0983260:CDS
CGTGGTATCTGACGGGACAT+TGG 0.510924 6_4:+26408183 Msa0983260:CDS
TAGAGCTTGAACGTGGGCCG+TGG 0.522206 6_4:+26408165 Msa0983260:CDS
AGAGGCTGTCCCAAAAGGAC+AGG 0.534725 6_4:+26407490 Msa0983260:CDS
GCTATGCGTTTAAGGGCATA+AGG 0.534942 6_4:-26408059 None:intergenic
GGAACGGACTATTCTCGGAT+TGG 0.537533 6_4:-26408255 None:intergenic
GACGGTTTAGAGCTTGAACG+TGG 0.549700 6_4:+26408158 Msa0983260:CDS
TCTACCCAATCAGCATGAAA+AGG 0.549978 6_4:-26407559 None:intergenic
CATTGGCCTCCCTCCCGCTA+CGG 0.557319 6_4:+26408200 Msa0983260:CDS
AGCGAGCCGTAGCGGGAGGG+AGG 0.562909 6_4:-26408206 None:intergenic
ACGTGGGCCGTGGTATCTGA+CGG 0.566135 6_4:+26408175 Msa0983260:CDS
GGCCCTATTGGATCTAGTAA+GGG 0.583375 6_4:+26407403 Msa0983260:five_prime_UTR
TTGGACACGCCTATAGCTAA+AGG 0.585840 6_4:-26408236 None:intergenic
CAGCATGAAAAGGCATTGAA+GGG 0.604261 6_4:-26407549 None:intergenic
CAATGTCCCGTCAGATACCA+CGG 0.604719 6_4:-26408182 None:intergenic
CAGGAGAGGAGCGAAGTGAG+AGG 0.607142 6_4:+26407966 Msa0983260:CDS
GTACAGCGAGCCGTAGCGGG+AGG 0.612644 6_4:-26408210 None:intergenic
TATTGGATCTAGTAAGGGGA+AGG 0.613431 6_4:+26407408 Msa0983260:five_prime_UTR
GAACGTACAGCGAGCCGTAG+CGG 0.616840 6_4:-26408214 None:intergenic
GCCCTATTGGATCTAGTAAG+GGG 0.621995 6_4:+26407404 Msa0983260:five_prime_UTR
ACGGTTTAGAGCTTGAACGT+GGG 0.631741 6_4:+26408159 Msa0983260:CDS
CTTCCTTGCACTATGTGGAA+CGG 0.640584 6_4:-26408271 None:intergenic
GAGAGGAGCGAAGTGAGAGG+CGG 0.650312 6_4:+26407969 Msa0983260:CDS
AGGACAGGTACAAGACGAAG+TGG 0.651118 6_4:+26407505 Msa0983260:CDS
GTCACTCGAGTTACAGGAGA+GGG 0.651239 6_4:+26408014 Msa0983260:CDS
CTCCTCTCCTGTAACTCTCG+CGG 0.659926 6_4:-26407954 None:intergenic
AGCCGCGAGAGTTACAGGAG+AGG 0.663478 6_4:+26407952 Msa0983260:CDS
CACATATGAGAAGTGAAACG+TGG 0.676128 6_4:-26408108 None:intergenic
TGTCACTCGAGTTACAGGAG+AGG 0.744502 6_4:+26408013 Msa0983260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAGAGTTTTGAAAGATGAA+AGG + chr6_4:26407443-26407462 Msa0983260:CDS 30.0%
!!! GTTTTGAAAGATGAAAGGAA+AGG + chr6_4:26407448-26407467 Msa0983260:CDS 30.0%
ACTAGATATAAGCTATAGGA+TGG - chr6_4:26407679-26407698 None:intergenic 30.0%
AGTCACTAGATATAAGCTAT+AGG - chr6_4:26407683-26407702 None:intergenic 30.0%
!! TGCTTTGAGTTGTATGTTTA+TGG + chr6_4:26407754-26407773 Msa0983260:intron 30.0%
!! GCTTTGAGTTGTATGTTTAT+GGG + chr6_4:26407755-26407774 Msa0983260:intron 30.0%
AGTTGTATGTTTATGGGTAA+GGG + chr6_4:26407761-26407780 Msa0983260:intron 30.0%
GGGGAAAGCAAATAAAATAA+AGG + chr6_4:26407781-26407800 Msa0983260:intron 30.0%
ATCTATCAAGAAGGATGAAT+AGG - chr6_4:26407812-26407831 None:intergenic 30.0%
GATGAAGAAATCTATCAAGA+AGG - chr6_4:26407821-26407840 None:intergenic 30.0%
! AAGAAGAAAGGAGACTTTTA+GGG - chr6_4:26408138-26408157 None:intergenic 30.0%
!!! TGAGAGTTTTGAAAGATGAA+AGG + chr6_4:26407443-26407462 Msa0983260:CDS 30.0%
!!! GTTTTGAAAGATGAAAGGAA+AGG + chr6_4:26407448-26407467 Msa0983260:CDS 30.0%
ACTAGATATAAGCTATAGGA+TGG - chr6_4:26407679-26407698 None:intergenic 30.0%
AGTCACTAGATATAAGCTAT+AGG - chr6_4:26407683-26407702 None:intergenic 30.0%
!! TGCTTTGAGTTGTATGTTTA+TGG + chr6_4:26407754-26407773 Msa0983260:intron 30.0%
!! GCTTTGAGTTGTATGTTTAT+GGG + chr6_4:26407755-26407774 Msa0983260:intron 30.0%
AGTTGTATGTTTATGGGTAA+GGG + chr6_4:26407761-26407780 Msa0983260:intron 30.0%
GGGGAAAGCAAATAAAATAA+AGG + chr6_4:26407781-26407800 Msa0983260:intron 30.0%
ATCTATCAAGAAGGATGAAT+AGG - chr6_4:26407812-26407831 None:intergenic 30.0%
GATGAAGAAATCTATCAAGA+AGG - chr6_4:26407821-26407840 None:intergenic 30.0%
! AAGAAGAAAGGAGACTTTTA+GGG - chr6_4:26408138-26408157 None:intergenic 30.0%
!! CTCTCTCTTCATCTTTTTCA+TGG - chr6_4:26407474-26407493 None:intergenic 35.0%
CATGAAAAAGATGAAGAGAG+AGG + chr6_4:26407472-26407491 Msa0983260:CDS 35.0%
!! AATGCCTTTTCATGCTGATT+GGG + chr6_4:26407555-26407574 Msa0983260:CDS 35.0%
GATAGCCTATATATAAGGTC+AGG - chr6_4:26407649-26407668 None:intergenic 35.0%
TTGGGGATAGCCTATATATA+AGG - chr6_4:26407654-26407673 None:intergenic 35.0%
! ATAAGCTATAGGATGGACTT+GGG - chr6_4:26407672-26407691 None:intergenic 35.0%
! TATAAGCTATAGGATGGACT+TGG - chr6_4:26407673-26407692 None:intergenic 35.0%
! TATCCTTTTCACTAAGCTCT+TGG + chr6_4:26407705-26407724 Msa0983260:intron 35.0%
ATTCCAAGAGCTTAGTGAAA+AGG - chr6_4:26407711-26407730 None:intergenic 35.0%
GAGTTGTATGTTTATGGGTA+AGG + chr6_4:26407760-26407779 Msa0983260:intron 35.0%
GTTGTATGTTTATGGGTAAG+GGG + chr6_4:26407762-26407781 Msa0983260:intron 35.0%
GCAACAGATCAATATGAATG+AGG - chr6_4:26407900-26407919 None:intergenic 35.0%
! AGAAGAAAGGAGACTTTTAG+GGG - chr6_4:26408137-26408156 None:intergenic 35.0%
! CAAGAAGAAAGGAGACTTTT+AGG - chr6_4:26408139-26408158 None:intergenic 35.0%
AAAGTCTCCTTTCTTCTTGA+CGG + chr6_4:26408140-26408159 Msa0983260:CDS 35.0%
!! CTCTCTCTTCATCTTTTTCA+TGG - chr6_4:26407474-26407493 None:intergenic 35.0%
CATGAAAAAGATGAAGAGAG+AGG + chr6_4:26407472-26407491 Msa0983260:CDS 35.0%
!! AATGCCTTTTCATGCTGATT+GGG + chr6_4:26407555-26407574 Msa0983260:CDS 35.0%
GATAGCCTATATATAAGGTC+AGG - chr6_4:26407649-26407668 None:intergenic 35.0%
TTGGGGATAGCCTATATATA+AGG - chr6_4:26407654-26407673 None:intergenic 35.0%
! ATAAGCTATAGGATGGACTT+GGG - chr6_4:26407672-26407691 None:intergenic 35.0%
! TATAAGCTATAGGATGGACT+TGG - chr6_4:26407673-26407692 None:intergenic 35.0%
! TATCCTTTTCACTAAGCTCT+TGG + chr6_4:26407705-26407724 Msa0983260:intron 35.0%
ATTCCAAGAGCTTAGTGAAA+AGG - chr6_4:26407711-26407730 None:intergenic 35.0%
GAGTTGTATGTTTATGGGTA+AGG + chr6_4:26407760-26407779 Msa0983260:intron 35.0%
GTTGTATGTTTATGGGTAAG+GGG + chr6_4:26407762-26407781 Msa0983260:intron 35.0%
GCAACAGATCAATATGAATG+AGG - chr6_4:26407900-26407919 None:intergenic 35.0%
! AGAAGAAAGGAGACTTTTAG+GGG - chr6_4:26408137-26408156 None:intergenic 35.0%
! CAAGAAGAAAGGAGACTTTT+AGG - chr6_4:26408139-26408158 None:intergenic 35.0%
AAAGTCTCCTTTCTTCTTGA+CGG + chr6_4:26408140-26408159 Msa0983260:CDS 35.0%
TCCCCTTACTAGATCCAATA+GGG - chr6_4:26407408-26407427 None:intergenic 40.0%
TTCCCCTTACTAGATCCAAT+AGG - chr6_4:26407409-26407428 None:intergenic 40.0%
TATTGGATCTAGTAAGGGGA+AGG + chr6_4:26407408-26407427 Msa0983260:five_prime_UTR 40.0%
!! CAGCATGAAAAGGCATTGAA+GGG - chr6_4:26407552-26407571 None:intergenic 40.0%
!! TCAGCATGAAAAGGCATTGA+AGG - chr6_4:26407553-26407572 None:intergenic 40.0%
! CAATGCCTTTTCATGCTGAT+TGG + chr6_4:26407554-26407573 Msa0983260:CDS 40.0%
TCTACCCAATCAGCATGAAA+AGG - chr6_4:26407562-26407581 None:intergenic 40.0%
GGGTAGAAGAAATTTGACCT+CGG + chr6_4:26407575-26407594 Msa0983260:intron 40.0%
AGAAGAAATTTGACCTCGGT+TGG + chr6_4:26407579-26407598 Msa0983260:intron 40.0%
ACCGACCTGACCTTATATAT+AGG + chr6_4:26407641-26407660 Msa0983260:intron 40.0%
GCCTATATATAAGGTCAGGT+CGG - chr6_4:26407645-26407664 None:intergenic 40.0%
! TAAGCTATAGGATGGACTTG+GGG - chr6_4:26407671-26407690 None:intergenic 40.0%
CTTCATCAACAAGCAAAGAG+AGG + chr6_4:26407834-26407853 Msa0983260:intron 40.0%
CAACAAGCAAAGAGAGGTTT+AGG + chr6_4:26407840-26407859 Msa0983260:intron 40.0%
CTGTTGAGAATCTTGGATCA+TGG - chr6_4:26407866-26407885 None:intergenic 40.0%
CATGATCCAAGATTCTCAAC+AGG + chr6_4:26407864-26407883 Msa0983260:intron 40.0%
CTTGTTCCTGTTGAGAATCT+TGG - chr6_4:26407873-26407892 None:intergenic 40.0%
CAAGATTCTCAACAGGAACA+AGG + chr6_4:26407871-26407890 Msa0983260:intron 40.0%
CGGATATGCTAGTCAAGATT+TGG + chr6_4:26407989-26408008 Msa0983260:CDS 40.0%
CACATATGAGAAGTGAAACG+TGG - chr6_4:26408111-26408130 None:intergenic 40.0%
CTCTAAACCGTCAAGAAGAA+AGG - chr6_4:26408150-26408169 None:intergenic 40.0%
CTGTACGTTCCTTTAGCTAT+AGG + chr6_4:26408227-26408246 Msa0983260:CDS 40.0%
TCCCCTTACTAGATCCAATA+GGG - chr6_4:26407408-26407427 None:intergenic 40.0%
TTCCCCTTACTAGATCCAAT+AGG - chr6_4:26407409-26407428 None:intergenic 40.0%
TATTGGATCTAGTAAGGGGA+AGG + chr6_4:26407408-26407427 Msa0983260:five_prime_UTR 40.0%
!! CAGCATGAAAAGGCATTGAA+GGG - chr6_4:26407552-26407571 None:intergenic 40.0%
!! TCAGCATGAAAAGGCATTGA+AGG - chr6_4:26407553-26407572 None:intergenic 40.0%
! CAATGCCTTTTCATGCTGAT+TGG + chr6_4:26407554-26407573 Msa0983260:CDS 40.0%
TCTACCCAATCAGCATGAAA+AGG - chr6_4:26407562-26407581 None:intergenic 40.0%
GGGTAGAAGAAATTTGACCT+CGG + chr6_4:26407575-26407594 Msa0983260:intron 40.0%
AGAAGAAATTTGACCTCGGT+TGG + chr6_4:26407579-26407598 Msa0983260:intron 40.0%
ACCGACCTGACCTTATATAT+AGG + chr6_4:26407641-26407660 Msa0983260:intron 40.0%
GCCTATATATAAGGTCAGGT+CGG - chr6_4:26407645-26407664 None:intergenic 40.0%
! TAAGCTATAGGATGGACTTG+GGG - chr6_4:26407671-26407690 None:intergenic 40.0%
CTTCATCAACAAGCAAAGAG+AGG + chr6_4:26407834-26407853 Msa0983260:intron 40.0%
CAACAAGCAAAGAGAGGTTT+AGG + chr6_4:26407840-26407859 Msa0983260:intron 40.0%
CTGTTGAGAATCTTGGATCA+TGG - chr6_4:26407866-26407885 None:intergenic 40.0%
CATGATCCAAGATTCTCAAC+AGG + chr6_4:26407864-26407883 Msa0983260:intron 40.0%
CTTGTTCCTGTTGAGAATCT+TGG - chr6_4:26407873-26407892 None:intergenic 40.0%
CAAGATTCTCAACAGGAACA+AGG + chr6_4:26407871-26407890 Msa0983260:intron 40.0%
CGGATATGCTAGTCAAGATT+TGG + chr6_4:26407989-26408008 Msa0983260:CDS 40.0%
CACATATGAGAAGTGAAACG+TGG - chr6_4:26408111-26408130 None:intergenic 40.0%
CTCTAAACCGTCAAGAAGAA+AGG - chr6_4:26408150-26408169 None:intergenic 40.0%
CTGTACGTTCCTTTAGCTAT+AGG + chr6_4:26408227-26408246 Msa0983260:CDS 40.0%
TGGCCCTATTGGATCTAGTA+AGG + chr6_4:26407402-26407421 Msa0983260:five_prime_UTR 45.0%
GGCCCTATTGGATCTAGTAA+GGG + chr6_4:26407403-26407422 Msa0983260:five_prime_UTR 45.0%
GCCCTATTGGATCTAGTAAG+GGG + chr6_4:26407404-26407423 Msa0983260:five_prime_UTR 45.0%
! CGTCTTGTACCTGTCCTTTT+GGG - chr6_4:26407502-26407521 None:intergenic 45.0%
! TCGTCTTGTACCTGTCCTTT+TGG - chr6_4:26407503-26407522 None:intergenic 45.0%
TCAAAGCAAGTGAGCAGCTT+GGG - chr6_4:26407742-26407761 None:intergenic 45.0%
TTGATCTGTTGCGAAGCTAC+AGG + chr6_4:26407908-26407927 Msa0983260:intron 45.0%
TGATCTGTTGCGAAGCTACA+GGG + chr6_4:26407909-26407928 Msa0983260:intron 45.0%
TTGGTTGTCACTCGAGTTAC+AGG + chr6_4:26408008-26408027 Msa0983260:CDS 45.0%
! GCTATGCGTTTAAGGGCATA+AGG - chr6_4:26408062-26408081 None:intergenic 45.0%
GCTGTCTGCTATGCGTTTAA+GGG - chr6_4:26408069-26408088 None:intergenic 45.0%
AGCTGTCTGCTATGCGTTTA+AGG - chr6_4:26408070-26408089 None:intergenic 45.0%
!! AGAAAGGAGACTTTTAGGGG+AGG - chr6_4:26408134-26408153 None:intergenic 45.0%
! ACGGTTTAGAGCTTGAACGT+GGG + chr6_4:26408159-26408178 Msa0983260:CDS 45.0%
TTGGACACGCCTATAGCTAA+AGG - chr6_4:26408239-26408258 None:intergenic 45.0%
TGGCCCTATTGGATCTAGTA+AGG + chr6_4:26407402-26407421 Msa0983260:five_prime_UTR 45.0%
GGCCCTATTGGATCTAGTAA+GGG + chr6_4:26407403-26407422 Msa0983260:five_prime_UTR 45.0%
GCCCTATTGGATCTAGTAAG+GGG + chr6_4:26407404-26407423 Msa0983260:five_prime_UTR 45.0%
! CGTCTTGTACCTGTCCTTTT+GGG - chr6_4:26407502-26407521 None:intergenic 45.0%
! TCGTCTTGTACCTGTCCTTT+TGG - chr6_4:26407503-26407522 None:intergenic 45.0%
TCAAAGCAAGTGAGCAGCTT+GGG - chr6_4:26407742-26407761 None:intergenic 45.0%
TTGATCTGTTGCGAAGCTAC+AGG + chr6_4:26407908-26407927 Msa0983260:intron 45.0%
TGATCTGTTGCGAAGCTACA+GGG + chr6_4:26407909-26407928 Msa0983260:intron 45.0%
TTGGTTGTCACTCGAGTTAC+AGG + chr6_4:26408008-26408027 Msa0983260:CDS 45.0%
! GCTATGCGTTTAAGGGCATA+AGG - chr6_4:26408062-26408081 None:intergenic 45.0%
GCTGTCTGCTATGCGTTTAA+GGG - chr6_4:26408069-26408088 None:intergenic 45.0%
AGCTGTCTGCTATGCGTTTA+AGG - chr6_4:26408070-26408089 None:intergenic 45.0%
!! AGAAAGGAGACTTTTAGGGG+AGG - chr6_4:26408134-26408153 None:intergenic 45.0%
! ACGGTTTAGAGCTTGAACGT+GGG + chr6_4:26408159-26408178 Msa0983260:CDS 45.0%
TTGGACACGCCTATAGCTAA+AGG - chr6_4:26408239-26408258 None:intergenic 45.0%
! AGTAAGGGGAAGGCGCATAA+TGG + chr6_4:26407418-26407437 Msa0983260:five_prime_UTR 50.0%
AAGAGAGAGGCTGTCCCAAA+AGG + chr6_4:26407485-26407504 Msa0983260:CDS 50.0%
AGGACAGGTACAAGACGAAG+TGG + chr6_4:26407505-26407524 Msa0983260:CDS 50.0%
CTCAAAGCAAGTGAGCAGCT+TGG - chr6_4:26407743-26407762 None:intergenic 50.0%
GATCTGTTGCGAAGCTACAG+GGG + chr6_4:26407910-26407929 Msa0983260:intron 50.0%
TGTCACTCGAGTTACAGGAG+AGG + chr6_4:26408013-26408032 Msa0983260:CDS 50.0%
GTCACTCGAGTTACAGGAGA+GGG + chr6_4:26408014-26408033 Msa0983260:CDS 50.0%
! GACGGTTTAGAGCTTGAACG+TGG + chr6_4:26408158-26408177 Msa0983260:CDS 50.0%
CAATGTCCCGTCAGATACCA+CGG - chr6_4:26408185-26408204 None:intergenic 50.0%
!! GGAACGGACTATTCTCGGAT+TGG - chr6_4:26408258-26408277 None:intergenic 50.0%
! AGTAAGGGGAAGGCGCATAA+TGG + chr6_4:26407418-26407437 Msa0983260:five_prime_UTR 50.0%
AAGAGAGAGGCTGTCCCAAA+AGG + chr6_4:26407485-26407504 Msa0983260:CDS 50.0%
AGGACAGGTACAAGACGAAG+TGG + chr6_4:26407505-26407524 Msa0983260:CDS 50.0%
CTCAAAGCAAGTGAGCAGCT+TGG - chr6_4:26407743-26407762 None:intergenic 50.0%
GATCTGTTGCGAAGCTACAG+GGG + chr6_4:26407910-26407929 Msa0983260:intron 50.0%
TGTCACTCGAGTTACAGGAG+AGG + chr6_4:26408013-26408032 Msa0983260:CDS 50.0%
GTCACTCGAGTTACAGGAGA+GGG + chr6_4:26408014-26408033 Msa0983260:CDS 50.0%
! GACGGTTTAGAGCTTGAACG+TGG + chr6_4:26408158-26408177 Msa0983260:CDS 50.0%
CAATGTCCCGTCAGATACCA+CGG - chr6_4:26408185-26408204 None:intergenic 50.0%
!! GGAACGGACTATTCTCGGAT+TGG - chr6_4:26408258-26408277 None:intergenic 50.0%
AGAGGCTGTCCCAAAAGGAC+AGG + chr6_4:26407490-26407509 Msa0983260:CDS 55.0%
! TTTGACCTCGGTTGGTGCAC+CGG + chr6_4:26407587-26407606 Msa0983260:intron 55.0%
CGTTAAGCCGCGAGAGTTAC+AGG + chr6_4:26407947-26407966 Msa0983260:CDS 55.0%
CTCCTCTCCTGTAACTCTCG+CGG - chr6_4:26407957-26407976 None:intergenic 55.0%
CGTGGTATCTGACGGGACAT+TGG + chr6_4:26408183-26408202 Msa0983260:CDS 55.0%
AGAGGCTGTCCCAAAAGGAC+AGG + chr6_4:26407490-26407509 Msa0983260:CDS 55.0%
! TTTGACCTCGGTTGGTGCAC+CGG + chr6_4:26407587-26407606 Msa0983260:intron 55.0%
CGTTAAGCCGCGAGAGTTAC+AGG + chr6_4:26407947-26407966 Msa0983260:CDS 55.0%
CTCCTCTCCTGTAACTCTCG+CGG - chr6_4:26407957-26407976 None:intergenic 55.0%
CGTGGTATCTGACGGGACAT+TGG + chr6_4:26408183-26408202 Msa0983260:CDS 55.0%
GTATACCGGTGCACCAACCG+AGG - chr6_4:26407595-26407614 None:intergenic 60.0%
ACCGGTATACCCCCTCCATC+AGG + chr6_4:26407605-26407624 Msa0983260:intron 60.0%
! CCCTGATGGAGGGGGTATAC+CGG - chr6_4:26407609-26407628 None:intergenic 60.0%
CCGGTATACCCCCTCCATCA+GGG + chr6_4:26407606-26407625 Msa0983260:intron 60.0%
CGGTATACCCCCTCCATCAG+GGG + chr6_4:26407607-26407626 Msa0983260:intron 60.0%
GCACAATCCCCTGATGGAGG+GGG - chr6_4:26407617-26407636 None:intergenic 60.0%
CGCACAATCCCCTGATGGAG+GGG - chr6_4:26407618-26407637 None:intergenic 60.0%
CCGCACAATCCCCTGATGGA+GGG - chr6_4:26407619-26407638 None:intergenic 60.0%
CCCTCCATCAGGGGATTGTG+CGG + chr6_4:26407616-26407635 Msa0983260:intron 60.0%
CCTCCATCAGGGGATTGTGC+GGG + chr6_4:26407617-26407636 Msa0983260:intron 60.0%
GTTCCCGCACAATCCCCTGA+TGG - chr6_4:26407623-26407642 None:intergenic 60.0%
AGCCGCGAGAGTTACAGGAG+AGG + chr6_4:26407952-26407971 Msa0983260:CDS 60.0%
CAGGAGAGGAGCGAAGTGAG+AGG + chr6_4:26407966-26407985 Msa0983260:CDS 60.0%
GAGAGGAGCGAAGTGAGAGG+CGG + chr6_4:26407969-26407988 Msa0983260:CDS 60.0%
TAGAGCTTGAACGTGGGCCG+TGG + chr6_4:26408165-26408184 Msa0983260:CDS 60.0%
ACGTGGGCCGTGGTATCTGA+CGG + chr6_4:26408175-26408194 Msa0983260:CDS 60.0%
AACGTACAGCGAGCCGTAGC+GGG - chr6_4:26408216-26408235 None:intergenic 60.0%
GAACGTACAGCGAGCCGTAG+CGG - chr6_4:26408217-26408236 None:intergenic 60.0%
GTATACCGGTGCACCAACCG+AGG - chr6_4:26407595-26407614 None:intergenic 60.0%
ACCGGTATACCCCCTCCATC+AGG + chr6_4:26407605-26407624 Msa0983260:intron 60.0%
! CCCTGATGGAGGGGGTATAC+CGG - chr6_4:26407609-26407628 None:intergenic 60.0%
CCGGTATACCCCCTCCATCA+GGG + chr6_4:26407606-26407625 Msa0983260:intron 60.0%
CGGTATACCCCCTCCATCAG+GGG + chr6_4:26407607-26407626 Msa0983260:intron 60.0%
GCACAATCCCCTGATGGAGG+GGG - chr6_4:26407617-26407636 None:intergenic 60.0%
CGCACAATCCCCTGATGGAG+GGG - chr6_4:26407618-26407637 None:intergenic 60.0%
CCGCACAATCCCCTGATGGA+GGG - chr6_4:26407619-26407638 None:intergenic 60.0%
CCCTCCATCAGGGGATTGTG+CGG + chr6_4:26407616-26407635 Msa0983260:intron 60.0%
CCTCCATCAGGGGATTGTGC+GGG + chr6_4:26407617-26407636 Msa0983260:intron 60.0%
GTTCCCGCACAATCCCCTGA+TGG - chr6_4:26407623-26407642 None:intergenic 60.0%
AGCCGCGAGAGTTACAGGAG+AGG + chr6_4:26407952-26407971 Msa0983260:CDS 60.0%
CAGGAGAGGAGCGAAGTGAG+AGG + chr6_4:26407966-26407985 Msa0983260:CDS 60.0%
GAGAGGAGCGAAGTGAGAGG+CGG + chr6_4:26407969-26407988 Msa0983260:CDS 60.0%
TAGAGCTTGAACGTGGGCCG+TGG + chr6_4:26408165-26408184 Msa0983260:CDS 60.0%
ACGTGGGCCGTGGTATCTGA+CGG + chr6_4:26408175-26408194 Msa0983260:CDS 60.0%
AACGTACAGCGAGCCGTAGC+GGG - chr6_4:26408216-26408235 None:intergenic 60.0%
GAACGTACAGCGAGCCGTAG+CGG - chr6_4:26408217-26408236 None:intergenic 60.0%
CCCGCACAATCCCCTGATGG+AGG - chr6_4:26407620-26407639 None:intergenic 65.0%
CGTGGGCCGTGGTATCTGAC+GGG + chr6_4:26408176-26408195 Msa0983260:CDS 65.0%
! CATTGGCCTCCCTCCCGCTA+CGG + chr6_4:26408200-26408219 Msa0983260:CDS 65.0%
! TACAGCGAGCCGTAGCGGGA+GGG - chr6_4:26408212-26408231 None:intergenic 65.0%
CCCGCACAATCCCCTGATGG+AGG - chr6_4:26407620-26407639 None:intergenic 65.0%
CGTGGGCCGTGGTATCTGAC+GGG + chr6_4:26408176-26408195 Msa0983260:CDS 65.0%
! CATTGGCCTCCCTCCCGCTA+CGG + chr6_4:26408200-26408219 Msa0983260:CDS 65.0%
! TACAGCGAGCCGTAGCGGGA+GGG - chr6_4:26408212-26408231 None:intergenic 65.0%
! GTACAGCGAGCCGTAGCGGG+AGG - chr6_4:26408213-26408232 None:intergenic 70.0%
! GTACAGCGAGCCGTAGCGGG+AGG - chr6_4:26408213-26408232 None:intergenic 70.0%
! AGCGAGCCGTAGCGGGAGGG+AGG - chr6_4:26408209-26408228 None:intergenic 75.0%
! AGCGAGCCGTAGCGGGAGGG+AGG - chr6_4:26408209-26408228 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 26407393 26408280 26407393 ID=Msa0983260;Name=Msa0983260
chr6_4 mRNA 26407393 26408280 26407393 ID=Msa0983260-mRNA-1;Parent=Msa0983260;Name=Msa0983260-mRNA-1;_AED=0.41;_eAED=0.41;_QI=49|0|0|1|1|1|2|0|162
chr6_4 exon 26407393 26407576 26407393 ID=Msa0983260-mRNA-1:exon:7433;Parent=Msa0983260-mRNA-1
chr6_4 exon 26407930 26408280 26407930 ID=Msa0983260-mRNA-1:exon:7434;Parent=Msa0983260-mRNA-1
chr6_4 five_prime_UTR 26407393 26407441 26407393 ID=Msa0983260-mRNA-1:five_prime_utr;Parent=Msa0983260-mRNA-1
chr6_4 CDS 26407442 26407576 26407442 ID=Msa0983260-mRNA-1:cds;Parent=Msa0983260-mRNA-1
chr6_4 CDS 26407930 26408280 26407930 ID=Msa0983260-mRNA-1:cds;Parent=Msa0983260-mRNA-1
Gene Sequence

>Msa0983260

ATGAGAGTTTTGAAAGATGAAAGGAAAGGCCATGAAAAAGATGAAGAGAGAGGCTGTCCCAAAAGGACAGGTACAAGACGAAGTGGCAGAAAGATAGAAGTCTTGCACCCTTCAATGCCTTTTCATGCTGATTGGGGGTGTACTCACTCGAGCGTTAAGCCGCGAGAGTTACAGGAGAGGAGCGAAGTGAGAGGCGGATATGCTAGTCAAGATTTGGTTGTCACTCGAGTTACAGGAGAGGGACTTCGTACGACTAACTTCGTACCTTATGCCCTTAAACGCATAGCAGACAGCTATCTTATTACTTTGAAAGCCACGTTTCACTTCTCATATGTGCCTCCCCTAAAAGTCTCCTTTCTTCTTGACGGTTTAGAGCTTGAACGTGGGCCGTGGTATCTGACGGGACATTGGCCTCCCTCCCGCTACGGCTCGCTGTACGTTCCTTTAGCTATAGGCGTGTCCAATCCGAGAATAGTCCGTTCCACA
Protein sequence

>Msa0983260

MRVLKDERKGHEKDEERGCPKRTGTRRSGRKIEVLHPSMPFHADWGCTHSSVKPRELQERSEVRGGYASQDLVVTRVTGEGLRTTNFVPYALKRIADSYLITLKATFHFSYVPPLKVSFLLDGLELERGPWYLTGHWPPSRYGSLYVPLAIGVSNPRIVRST