AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0908840


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 40 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAAAAGAAAGTAGGTTTC+GGG 0.299621 6_2:+9933127 Msa0908840:intron
TGCGCGAGTCACCTTCTTCT+AGG 0.325482 6_2:+9933304 Msa0908840:CDS
ACCAGCCAGCTGGGTTGGTT+TGG 0.332153 6_2:+9933245 Msa0908840:CDS
CGCAATGGCTTTCTCTGATA+GGG 0.371132 6_2:+9932839 None:intergenic
ATTCCTTTCTATTGAAAAGA+AGG 0.387353 6_2:+9933270 Msa0908840:CDS
GCGCAATGGCTTTCTCTGAT+AGG 0.391358 6_2:+9932838 None:intergenic
GTCGTCCGTCTCAGGTTTGC+CGG 0.398406 6_2:+9933201 Msa0908840:CDS
GGACCGTGGTCGTCCGTCTC+AGG 0.441663 6_2:+9933193 Msa0908840:CDS
AGCAGCACCTTGTATAGGTT+GGG 0.447543 6_2:-9932882 None:intergenic
GTACACCTCCGGTGCTGATA+AGG 0.450222 6_2:+9933167 Msa0908840:CDS
GATTGACCAGCCAGCTGGGT+TGG 0.459599 6_2:+9933240 Msa0908840:CDS
GTCAATCTCAGAGATCTTAT+CGG 0.462954 6_2:-9933224 None:intergenic
AAGCGAGCAGCACCTTGTAT+AGG 0.473472 6_2:-9932887 None:intergenic
TCTGAGATTGACCAGCCAGC+TGG 0.486873 6_2:+9933235 Msa0908840:CDS
TTCATTGATTAGTACACCTC+CGG 0.492064 6_2:+9933156 Msa0908840:CDS
CGCTTTCTCTCAGCCACCAG+TGG 0.505851 6_2:+9932905 Msa0908840:CDS
CTGAGATTGACCAGCCAGCT+GGG 0.514570 6_2:+9933236 Msa0908840:CDS
CACCTTCTTCTAGGCTCCGC+TGG 0.516734 6_2:+9933313 Msa0908840:CDS
GCCAAACCAACCCAGCTGGC+TGG 0.523772 6_2:-9933246 None:intergenic
GAGCAGCACCTTGTATAGGT+TGG 0.534433 6_2:-9932883 None:intergenic
ATCTCAGAGATCTTATCGGC+CGG 0.550066 6_2:-9933220 None:intergenic
AATAGCCAAACCAACCCAGC+TGG 0.553661 6_2:-9933250 None:intergenic
CCTCCGGTGCTGATAAGGTC+GGG 0.561319 6_2:+9933172 Msa0908840:CDS
ATCGGCCGGCAAACCTGAGA+CGG 0.579939 6_2:-9933206 None:intergenic
GCCACCAGTGGCTTATGTAG+TGG 0.581920 6_2:+9932917 Msa0908840:CDS
TGCTGATAAGGTCGGGACCG+TGG 0.590314 6_2:+9933179 Msa0908840:CDS
GTCCAGCGGAGCCTAGAAGA+AGG 0.604022 6_2:-9933315 None:intergenic
CTAGGCTCCGCTGGACGACA+CGG 0.612977 6_2:+9933322 Msa0908840:CDS
GAGAATGCCGTGTCGTCCAG+CGG 0.617479 6_2:-9933329 None:intergenic
CCCGACCTTATCAGCACCGG+AGG 0.624401 6_2:-9933172 None:intergenic
AGCACCTTGTATAGGTTGGG+CGG 0.628644 6_2:-9932879 None:intergenic
AGCTCCGCCCAACCTATACA+AGG 0.631063 6_2:+9932875 Msa0908840:CDS
CCGACCACTACATAAGCCAC+TGG 0.631161 6_2:-9932921 None:intergenic
ACCTCCGGTGCTGATAAGGT+CGG 0.637828 6_2:+9933171 Msa0908840:CDS
GCAATGGCTTTCTCTGATAG+GGG 0.641776 6_2:+9932840 None:intergenic
GGTCCCGACCTTATCAGCAC+CGG 0.654281 6_2:-9933175 None:intergenic
AAACCTGAGACGGACGACCA+CGG 0.659367 6_2:-9933196 None:intergenic
ACCACTACATAAGCCACTGG+TGG 0.667213 6_2:-9932918 None:intergenic
AGAAAAGAAAGTAGGTTTCG+GGG 0.671452 6_2:+9933128 Msa0908840:intron
GAAAAGAAAGTAGGTTTCGG+GGG 0.676234 6_2:+9933129 Msa0908840:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTTGTCAGAAAAGAAAGT+AGG + chr6_2:9933120-9933139 Msa0908840:intron 25.0%
! ATTCCTTTCTATTGAAAAGA+AGG + chr6_2:9933270-9933289 Msa0908840:CDS 25.0%
TCAGAAAAGAAAGTAGGTTT+CGG + chr6_2:9933126-9933145 Msa0908840:intron 30.0%
! ACTCCTTCTTTTCAATAGAA+AGG - chr6_2:9933276-9933295 None:intergenic 30.0%
ACCTTTGGATTCTTAAGTAG+GGG - chr6_2:9932970-9932989 None:intergenic 35.0%
GACCTTTGGATTCTTAAGTA+GGG - chr6_2:9932971-9932990 None:intergenic 35.0%
TGACCTTTGGATTCTTAAGT+AGG - chr6_2:9932972-9932991 None:intergenic 35.0%
GTTGTTGTGATTGAGTTTGA+GGG - chr6_2:9933074-9933093 None:intergenic 35.0%
TGTTGTTGTGATTGAGTTTG+AGG - chr6_2:9933075-9933094 None:intergenic 35.0%
CAGAAAAGAAAGTAGGTTTC+GGG + chr6_2:9933127-9933146 Msa0908840:intron 35.0%
AGAAAAGAAAGTAGGTTTCG+GGG + chr6_2:9933128-9933147 Msa0908840:intron 35.0%
! TTCATTGATTAGTACACCTC+CGG + chr6_2:9933156-9933175 Msa0908840:CDS 35.0%
GTCAATCTCAGAGATCTTAT+CGG - chr6_2:9933227-9933246 None:intergenic 35.0%
CCTTTGGATTCTTAAGTAGG+GGG - chr6_2:9932969-9932988 None:intergenic 40.0%
AAGAATCCAAAGGTCACCTT+TGG + chr6_2:9932976-9932995 Msa0908840:intron 40.0%
GAAAAGAAAGTAGGTTTCGG+GGG + chr6_2:9933129-9933148 Msa0908840:intron 40.0%
AGCAGCACCTTGTATAGGTT+GGG - chr6_2:9932885-9932904 None:intergenic 45.0%
TTCTTAAGTAGGGGGCAAAG+AGG - chr6_2:9932961-9932980 None:intergenic 45.0%
CCCCCTACTTAAGAATCCAA+AGG + chr6_2:9932966-9932985 Msa0908840:intron 45.0%
GCTTTGGTTACGAAAGTAGC+CGG + chr6_2:9933012-9933031 Msa0908840:intron 45.0%
CTTTGGTTACGAAAGTAGCC+GGG + chr6_2:9933013-9933032 Msa0908840:intron 45.0%
TTTGGTTACGAAAGTAGCCG+GGG + chr6_2:9933014-9933033 Msa0908840:intron 45.0%
TCACAACAACATGTCGTGAC+CGG + chr6_2:9933084-9933103 Msa0908840:intron 45.0%
CAAAATCCACCAAGCCTAAC+CGG - chr6_2:9933106-9933125 None:intergenic 45.0%
ATCTCAGAGATCTTATCGGC+CGG - chr6_2:9933223-9933242 None:intergenic 45.0%
! AGCACCTTGTATAGGTTGGG+CGG - chr6_2:9932882-9932901 None:intergenic 50.0%
GAGCAGCACCTTGTATAGGT+TGG - chr6_2:9932886-9932905 None:intergenic 50.0%
AAGCGAGCAGCACCTTGTAT+AGG - chr6_2:9932890-9932909 None:intergenic 50.0%
ACCACTACATAAGCCACTGG+TGG - chr6_2:9932921-9932940 None:intergenic 50.0%
CCAGTGGCTTATGTAGTGGT+CGG + chr6_2:9932921-9932940 Msa0908840:intron 50.0%
CAACAGCCAAAGGTGACCTT+TGG - chr6_2:9932985-9933004 None:intergenic 50.0%
AGCGTCAAGACAACAGCCAA+AGG - chr6_2:9932995-9933014 None:intergenic 50.0%
TGGCTGTTGTCTTGACGCTT+TGG + chr6_2:9932996-9933015 Msa0908840:intron 50.0%
CCATCCATAAACCTAGACCC+CGG - chr6_2:9933034-9933053 None:intergenic 50.0%
ACAACATGTCGTGACCGGTT+AGG + chr6_2:9933089-9933108 Msa0908840:intron 50.0%
AAGAAAGTAGGTTTCGGGGG+TGG + chr6_2:9933132-9933151 Msa0908840:intron 50.0%
AGAAAGTAGGTTTCGGGGGT+GGG + chr6_2:9933133-9933152 Msa0908840:intron 50.0%
AATAGCCAAACCAACCCAGC+TGG - chr6_2:9933253-9933272 None:intergenic 50.0%
AGCTCCGCCCAACCTATACA+AGG + chr6_2:9932875-9932894 Msa0908840:CDS 55.0%
GCCACCAGTGGCTTATGTAG+TGG + chr6_2:9932917-9932936 Msa0908840:CDS 55.0%
CCGACCACTACATAAGCCAC+TGG - chr6_2:9932924-9932943 None:intergenic 55.0%
TACGAAAGTAGCCGGGGTCT+AGG + chr6_2:9933020-9933039 Msa0908840:intron 55.0%
GTAGCCGGGGTCTAGGTTTA+TGG + chr6_2:9933027-9933046 Msa0908840:intron 55.0%
CCGGGGTCTAGGTTTATGGA+TGG + chr6_2:9933031-9933050 Msa0908840:intron 55.0%
ATGTCGTGACCGGTTAGGCT+TGG + chr6_2:9933094-9933113 Msa0908840:intron 55.0%
!! GTACACCTCCGGTGCTGATA+AGG + chr6_2:9933167-9933186 Msa0908840:CDS 55.0%
!! ACCTCCGGTGCTGATAAGGT+CGG + chr6_2:9933171-9933190 Msa0908840:CDS 55.0%
AAACCTGAGACGGACGACCA+CGG - chr6_2:9933199-9933218 None:intergenic 55.0%
! TCTGAGATTGACCAGCCAGC+TGG + chr6_2:9933235-9933254 Msa0908840:CDS 55.0%
CTGAGATTGACCAGCCAGCT+GGG + chr6_2:9933236-9933255 Msa0908840:CDS 55.0%
TGCGCGAGTCACCTTCTTCT+AGG + chr6_2:9933304-9933323 Msa0908840:CDS 55.0%
CGCTTTCTCTCAGCCACCAG+TGG + chr6_2:9932905-9932924 Msa0908840:CDS 60.0%
TCGTGACCGGTTAGGCTTGG+TGG + chr6_2:9933097-9933116 Msa0908840:intron 60.0%
!! CCTCCGGTGCTGATAAGGTC+GGG + chr6_2:9933172-9933191 Msa0908840:CDS 60.0%
GGTCCCGACCTTATCAGCAC+CGG - chr6_2:9933178-9933197 None:intergenic 60.0%
TGCTGATAAGGTCGGGACCG+TGG + chr6_2:9933179-9933198 Msa0908840:CDS 60.0%
GTCGTCCGTCTCAGGTTTGC+CGG + chr6_2:9933201-9933220 Msa0908840:CDS 60.0%
ATCGGCCGGCAAACCTGAGA+CGG - chr6_2:9933209-9933228 None:intergenic 60.0%
GATTGACCAGCCAGCTGGGT+TGG + chr6_2:9933240-9933259 Msa0908840:CDS 60.0%
!! ACCAGCCAGCTGGGTTGGTT+TGG + chr6_2:9933245-9933264 Msa0908840:CDS 60.0%
CACCTTCTTCTAGGCTCCGC+TGG + chr6_2:9933313-9933332 Msa0908840:CDS 60.0%
GTCCAGCGGAGCCTAGAAGA+AGG - chr6_2:9933318-9933337 None:intergenic 60.0%
GAGAATGCCGTGTCGTCCAG+CGG - chr6_2:9933332-9933351 None:intergenic 60.0%
! GGGGGCAAAGAGGAGCACGT+AGG - chr6_2:9932951-9932970 None:intergenic 65.0%
! CCCGACCTTATCAGCACCGG+AGG - chr6_2:9933175-9933194 None:intergenic 65.0%
GCCAAACCAACCCAGCTGGC+TGG - chr6_2:9933249-9933268 None:intergenic 65.0%
CTAGGCTCCGCTGGACGACA+CGG + chr6_2:9933322-9933341 Msa0908840:CDS 65.0%
GGACCGTGGTCGTCCGTCTC+AGG + chr6_2:9933193-9933212 Msa0908840:CDS 70.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 9932843 9933357 9932843 ID=Msa0908840;Name=Msa0908840
chr6_2 mRNA 9932843 9933357 9932843 ID=Msa0908840-mRNA-1;Parent=Msa0908840;Name=Msa0908840-mRNA-1;_AED=0.27;_eAED=0.39;_QI=0|0|0|1|0|0|2|0|103
chr6_2 exon 9932843 9932938 9932843 ID=Msa0908840-mRNA-1:exon:2502;Parent=Msa0908840-mRNA-1
chr6_2 exon 9933142 9933357 9933142 ID=Msa0908840-mRNA-1:exon:2503;Parent=Msa0908840-mRNA-1
chr6_2 CDS 9932843 9932938 9932843 ID=Msa0908840-mRNA-1:cds;Parent=Msa0908840-mRNA-1
chr6_2 CDS 9933142 9933357 9933142 ID=Msa0908840-mRNA-1:cds;Parent=Msa0908840-mRNA-1
Gene Sequence

>Msa0908840

ATGGCTTTCTCTGATAGGGGCTCGCTTCTTCAAGCTCCGCCCAACCTATACAAGGTGCTGCTCGCTTTCTCTCAGCCACCAGTGGCTTATGTAGTGGTTTCGGGGGTGGGTTCATTGATTAGTACACCTCCGGTGCTGATAAGGTCGGGACCGTGGTCGTCCGTCTCAGGTTTGCCGGCCGATAAGATCTCTGAGATTGACCAGCCAGCTGGGTTGGTTTGGCTATTCCTTTCTATTGAAAAGAAGGAGTCAGCTGTCTGCGCGAGTCACCTTCTTCTAGGCTCCGCTGGACGACACGGCATTCTCGTTTAA

Protein sequence

>Msa0908840

MAFSDRGSLLQAPPNLYKVLLAFSQPPVAYVVVSGVGSLISTPPVLIRSGPWSSVSGLPADKISEIDQPAGLVWLFLSIEKKESAVCASHLLLGSAGRHGILV*