AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0908920


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0908920 MtrunA17_Chr3g0094961 99.522 209 1 0 1 209 57 265 2.51e-150 417
Msa0908920 MtrunA17_MTg0491471 99.522 209 1 0 1 209 57 265 2.51e-150 417
Msa0908920 MtrunA17_Chr6g0461571 92.169 166 8 1 1 166 57 217 6.79e-105 301
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0908920 AT2G07687.1 94.737 209 11 0 1 209 57 265 7.47e-134 376
Msa0908920 ATMG00730.1 94.737 209 11 0 1 209 57 265 7.47e-134 376

Find 62 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTATTTCGGATAGTATTTA+TGG 0.126182 6_2:-9990264 Msa0908920:CDS
TTCTTAGCAACTGGCTTTCA+TGG 0.230792 6_2:-9990231 Msa0908920:CDS
GGCTTCTTCTCATTCTTCTT+TGG 0.238024 6_2:-9990526 Msa0908920:CDS
TCCCATTTGTCTCTATCTAT+TGG 0.241271 6_2:-9990071 Msa0908920:CDS
TCTAAAACCCAAATCCCTTT+TGG 0.290653 6_2:+9990471 None:intergenic
TCAAGCACCCTTCACTATTT+CGG 0.297061 6_2:-9990277 Msa0908920:CDS
CATCCGGAGCAGCCGTAACT+TGG 0.313664 6_2:-9990407 Msa0908920:CDS
AAAGCCAGTTGCTAAGAAAA+AGG 0.364689 6_2:+9990236 None:intergenic
CTAGTATTCACTGGCTTTCA+AGG 0.370584 6_2:-9990312 Msa0908920:CDS
TCCTATATACCATGTTTGTA+TGG 0.372510 6_2:-9990674 None:intergenic
CTCCGGATGAAAGGAGAATA+GGG 0.375707 6_2:+9990421 None:intergenic
CATACCAAAGTAGTACAATT+AGG 0.379974 6_2:-9990612 Msa0908920:CDS
AGAATAGGGGTATTAAGAAA+TGG 0.383171 6_2:+9990435 None:intergenic
TGGCACCTACGGTAGAGATC+GGG 0.410967 6_2:-9990506 Msa0908920:CDS
TGGTCAGATGACCAAGATAT+TGG 0.422687 6_2:+9990160 None:intergenic
TTGAAGCAGCTGCATGGTAC+TGG 0.426522 6_2:-9990119 Msa0908920:CDS
CATTTGTCTCTATCTATTGG+TGG 0.430734 6_2:-9990068 Msa0908920:CDS
GAATAGGGGTATTAAGAAAT+GGG 0.443493 6_2:+9990436 None:intergenic
GCTCCGGATGAAAGGAGAAT+AGG 0.447641 6_2:+9990420 None:intergenic
ACCCCTATTCTCCTTTCATC+CGG 0.453687 6_2:-9990423 Msa0908920:CDS
AGCCCAAGTTACGGCTGCTC+CGG 0.457418 6_2:+9990404 None:intergenic
ATTTGTCTCTATCTATTGGT+GGG 0.468958 6_2:-9990067 Msa0908920:CDS
ACCAATAGATAGAGACAAAT+GGG 0.470792 6_2:+9990070 None:intergenic
TGTGGTATTCGCCAATATCT+TGG 0.472546 6_2:-9990171 Msa0908920:CDS
CACCAATAGATAGAGACAAA+TGG 0.475440 6_2:+9990069 None:intergenic
TTGGCACCTACGGTAGAGAT+CGG 0.478668 6_2:-9990507 Msa0908920:CDS
GTACAATTAGGACCTCGATA+TGG 0.480031 6_2:-9990600 Msa0908920:CDS
ATTCACTGGCTTTCAAGGAA+TGG 0.480527 6_2:-9990307 Msa0908920:CDS
AGGTCCTAATTGTACTACTT+TGG 0.481047 6_2:+9990608 None:intergenic
TTTAGTAGCAACCGTTTCAC+TGG 0.485093 6_2:-9990337 Msa0908920:CDS
AAAGCCAACGTGATGCTCCT+TGG 0.488653 6_2:+9990140 None:intergenic
TCACTGGCTCTAGTATTCAC+TGG 0.492013 6_2:-9990321 Msa0908920:CDS
GTCTCTATCTATTGGTGGGG+AGG 0.494921 6_2:-9990063 Msa0908920:CDS
TTCTATTCCGTTCATCGTAT+CGG 0.511656 6_2:-9990577 Msa0908920:CDS
TCATTCTTCTTTGGCACCTA+CGG 0.520018 6_2:-9990517 Msa0908920:CDS
TTACGGCTGCTCCGGATGAA+AGG 0.531682 6_2:+9990412 None:intergenic
ATCCGGAGCAGCCGTAACTT+GGG 0.536168 6_2:-9990406 Msa0908920:CDS
GGCTCATCATGCTATACTCG+CGG 0.537898 6_2:-9990385 Msa0908920:CDS
AGCATGATGAGCCCAAGTTA+CGG 0.549704 6_2:+9990395 None:intergenic
TCATGCTATACTCGCGGGGA+AGG 0.556298 6_2:-9990379 Msa0908920:CDS
GCTCATCATGCTATACTCGC+GGG 0.559120 6_2:-9990384 Msa0908920:CDS
CTACGTGAATCCACGTTGGA+AGG 0.559738 6_2:-9990639 Msa0908920:CDS
AATACTATCCGAAATAGTGA+AGG 0.567530 6_2:+9990269 None:intergenic
TTTGTCTCTATCTATTGGTG+GGG 0.568228 6_2:-9990066 Msa0908920:CDS
GAATACTAGAGCCAGTGAAA+CGG 0.581702 6_2:+9990326 None:intergenic
ATTGGTGGGGAGGTATATGA+AGG 0.582028 6_2:-9990053 None:intergenic
TATTCCGTTCATCGTATCGG+AGG 0.604181 6_2:-9990574 Msa0908920:CDS
GGCACCTACGGTAGAGATCG+GGG 0.615963 6_2:-9990505 Msa0908920:CDS
TATATACCATGTTTGTATGG+TGG 0.617991 6_2:-9990671 None:intergenic
TTGGCTTTGAAGCAGCTGCA+TGG 0.631703 6_2:-9990125 Msa0908920:CDS
TTAAGAAATGGGATTTCCCG+AGG 0.638861 6_2:+9990447 None:intergenic
CTCATCATGCTATACTCGCG+GGG 0.640810 6_2:-9990383 Msa0908920:CDS
ATACTATCCGAAATAGTGAA+GGG 0.643332 6_2:+9990270 None:intergenic
TCCGGATGAAAGGAGAATAG+GGG 0.663915 6_2:+9990422 None:intergenic
ATAACCTCCGATACGATGAA+CGG 0.668960 6_2:+9990570 None:intergenic
ATCGCGCCACCATACAAACA+TGG 0.674686 6_2:+9990665 None:intergenic
GGTATGATGTCCTTCCAACG+TGG 0.683344 6_2:+9990629 None:intergenic
ATATCTTGGTCATCTGACCA+AGG 0.693931 6_2:-9990157 Msa0908920:CDS
GCACCTACGGTAGAGATCGG+GGG 0.709010 6_2:-9990504 Msa0908920:CDS
TGTTCTACGTGAATCCACGT+TGG 0.713103 6_2:-9990643 Msa0908920:CDS
CTGACCAAGGAGCATCACGT+TGG 0.717728 6_2:-9990144 Msa0908920:CDS
AACGGAATAGAACCATATCG+AGG 0.721827 6_2:+9990588 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTCCTTTTTGCTTTTTTT+CGG - chr6_2:9990171-9990190 Msa0908920:CDS 25.0%
! ACTATTTCGGATAGTATTTA+TGG - chr6_2:9990455-9990474 Msa0908920:CDS 25.0%
CATACCAAAGTAGTACAATT+AGG - chr6_2:9990107-9990126 Msa0908920:CDS 30.0%
!!! GTTCCTTTTTGCTTTTTTTC+GGG - chr6_2:9990172-9990191 Msa0908920:CDS 30.0%
!! GAATAGGGGTATTAAGAAAT+GGG + chr6_2:9990286-9990305 None:intergenic 30.0%
!! AGAATAGGGGTATTAAGAAA+TGG + chr6_2:9990287-9990306 None:intergenic 30.0%
ATACTATCCGAAATAGTGAA+GGG + chr6_2:9990452-9990471 None:intergenic 30.0%
AATACTATCCGAAATAGTGA+AGG + chr6_2:9990453-9990472 None:intergenic 30.0%
! CATGGTTTTCATGTGATTAT+AGG - chr6_2:9990506-9990525 Msa0908920:CDS 30.0%
ACCAATAGATAGAGACAAAT+GGG + chr6_2:9990652-9990671 None:intergenic 30.0%
ATTTGTCTCTATCTATTGGT+GGG - chr6_2:9990652-9990671 Msa0908920:CDS 30.0%
AGGTCCTAATTGTACTACTT+TGG + chr6_2:9990114-9990133 None:intergenic 35.0%
TTCTATTCCGTTCATCGTAT+CGG - chr6_2:9990142-9990161 Msa0908920:CDS 35.0%
AAGCCCGAAAAAAAGCAAAA+AGG + chr6_2:9990178-9990197 None:intergenic 35.0%
! TAAAACCCAAATCCCTTTTG+GGG + chr6_2:9990249-9990268 None:intergenic 35.0%
! CTAAAACCCAAATCCCTTTT+GGG + chr6_2:9990250-9990269 None:intergenic 35.0%
!! TCTAAAACCCAAATCCCTTT+TGG + chr6_2:9990251-9990270 None:intergenic 35.0%
! TCTACCTTTTTCTTAGCAAC+TGG - chr6_2:9990479-9990498 Msa0908920:CDS 35.0%
AAAGCCAGTTGCTAAGAAAA+AGG + chr6_2:9990486-9990505 None:intergenic 35.0%
! ACTCTTTTCTTGATCGTATG+TGG - chr6_2:9990530-9990549 Msa0908920:CDS 35.0%
TCCCATTTGTCTCTATCTAT+TGG - chr6_2:9990648-9990667 Msa0908920:CDS 35.0%
CACCAATAGATAGAGACAAA+TGG + chr6_2:9990653-9990672 None:intergenic 35.0%
CATTTGTCTCTATCTATTGG+TGG - chr6_2:9990651-9990670 Msa0908920:CDS 35.0%
TTTGTCTCTATCTATTGGTG+GGG - chr6_2:9990653-9990672 Msa0908920:CDS 35.0%
GTACAATTAGGACCTCGATA+TGG - chr6_2:9990119-9990138 Msa0908920:CDS 40.0%
AACGGAATAGAACCATATCG+AGG + chr6_2:9990134-9990153 None:intergenic 40.0%
ATAACCTCCGATACGATGAA+CGG + chr6_2:9990152-9990171 None:intergenic 40.0%
GGCTTCTTCTCATTCTTCTT+TGG - chr6_2:9990193-9990212 Msa0908920:CDS 40.0%
TCATTCTTCTTTGGCACCTA+CGG - chr6_2:9990202-9990221 Msa0908920:CDS 40.0%
! AAAACCCAAATCCCTTTTGG+GGG + chr6_2:9990248-9990267 None:intergenic 40.0%
!!! GGATTTGGGTTTTAGATCCT+CGG - chr6_2:9990255-9990274 Msa0908920:CDS 40.0%
!!! GATTTGGGTTTTAGATCCTC+GGG - chr6_2:9990256-9990275 Msa0908920:CDS 40.0%
TTAAGAAATGGGATTTCCCG+AGG + chr6_2:9990275-9990294 None:intergenic 40.0%
TTTAGTAGCAACCGTTTCAC+TGG - chr6_2:9990382-9990401 Msa0908920:CDS 40.0%
GAATACTAGAGCCAGTGAAA+CGG + chr6_2:9990396-9990415 None:intergenic 40.0%
!! CTAGTATTCACTGGCTTTCA+AGG - chr6_2:9990407-9990426 Msa0908920:CDS 40.0%
!! ATTCACTGGCTTTCAAGGAA+TGG - chr6_2:9990412-9990431 Msa0908920:CDS 40.0%
TCAAGCACCCTTCACTATTT+CGG - chr6_2:9990442-9990461 Msa0908920:CDS 40.0%
!! TTCTTAGCAACTGGCTTTCA+TGG - chr6_2:9990488-9990507 Msa0908920:CDS 40.0%
TGTGGTATTCGCCAATATCT+TGG - chr6_2:9990548-9990567 Msa0908920:CDS 40.0%
TGGTCAGATGACCAAGATAT+TGG + chr6_2:9990562-9990581 None:intergenic 40.0%
ATATCTTGGTCATCTGACCA+AGG - chr6_2:9990562-9990581 Msa0908920:CDS 40.0%
TGTTCTACGTGAATCCACGT+TGG - chr6_2:9990076-9990095 Msa0908920:CDS 45.0%
TATTCCGTTCATCGTATCGG+AGG - chr6_2:9990145-9990164 Msa0908920:CDS 45.0%
ACCCCTATTCTCCTTTCATC+CGG - chr6_2:9990296-9990315 Msa0908920:CDS 45.0%
TCCGGATGAAAGGAGAATAG+GGG + chr6_2:9990300-9990319 None:intergenic 45.0%
CTCCGGATGAAAGGAGAATA+GGG + chr6_2:9990301-9990320 None:intergenic 45.0%
AGCATGATGAGCCCAAGTTA+CGG + chr6_2:9990327-9990346 None:intergenic 45.0%
!! TCACTGGCTCTAGTATTCAC+TGG - chr6_2:9990398-9990417 Msa0908920:CDS 45.0%
!! GTACTGGCATTTTGTAGACG+TGG - chr6_2:9990616-9990635 Msa0908920:CDS 45.0%
! GGCATTTTGTAGACGTGGTT+CGG - chr6_2:9990621-9990640 Msa0908920:CDS 45.0%
CTACGTGAATCCACGTTGGA+AGG - chr6_2:9990080-9990099 Msa0908920:CDS 50.0%
GGTATGATGTCCTTCCAACG+TGG + chr6_2:9990093-9990112 None:intergenic 50.0%
TTGGCACCTACGGTAGAGAT+CGG - chr6_2:9990212-9990231 Msa0908920:CDS 50.0%
TTTGGCCCCCAAAAGGGATT+TGG - chr6_2:9990240-9990259 Msa0908920:CDS 50.0%
TTGGCCCCCAAAAGGGATTT+GGG - chr6_2:9990241-9990260 Msa0908920:CDS 50.0%
GCTCCGGATGAAAGGAGAAT+AGG + chr6_2:9990302-9990321 None:intergenic 50.0%
GGCTCATCATGCTATACTCG+CGG - chr6_2:9990334-9990353 Msa0908920:CDS 50.0%
GCTCATCATGCTATACTCGC+GGG - chr6_2:9990335-9990354 Msa0908920:CDS 50.0%
CTCATCATGCTATACTCGCG+GGG - chr6_2:9990336-9990355 Msa0908920:CDS 50.0%
AAAGCCAACGTGATGCTCCT+TGG + chr6_2:9990582-9990601 None:intergenic 50.0%
!! TTGGCTTTGAAGCAGCTGCA+TGG - chr6_2:9990594-9990613 Msa0908920:CDS 50.0%
TTGAAGCAGCTGCATGGTAC+TGG - chr6_2:9990600-9990619 Msa0908920:CDS 50.0%
GTCTCTATCTATTGGTGGGG+AGG - chr6_2:9990656-9990675 Msa0908920:CDS 50.0%
TGGCACCTACGGTAGAGATC+GGG - chr6_2:9990213-9990232 Msa0908920:CDS 55.0%
ATACCCCCGATCTCTACCGT+AGG + chr6_2:9990221-9990240 None:intergenic 55.0%
!! CGGTAGAGATCGGGGGTATT+TGG - chr6_2:9990222-9990241 Msa0908920:CDS 55.0%
! GGGGTATTTGGCCCCCAAAA+GGG - chr6_2:9990234-9990253 Msa0908920:CDS 55.0%
TTACGGCTGCTCCGGATGAA+AGG + chr6_2:9990310-9990329 None:intergenic 55.0%
ATCCGGAGCAGCCGTAACTT+GGG - chr6_2:9990313-9990332 Msa0908920:CDS 55.0%
TCATGCTATACTCGCGGGGA+AGG - chr6_2:9990340-9990359 Msa0908920:CDS 55.0%
!! CTGACCAAGGAGCATCACGT+TGG - chr6_2:9990575-9990594 Msa0908920:CDS 55.0%
GGCACCTACGGTAGAGATCG+GGG - chr6_2:9990214-9990233 Msa0908920:CDS 60.0%
GCACCTACGGTAGAGATCGG+GGG - chr6_2:9990215-9990234 Msa0908920:CDS 60.0%
!! GGGGGTATTTGGCCCCCAAA+AGG - chr6_2:9990233-9990252 Msa0908920:CDS 60.0%
CATCCGGAGCAGCCGTAACT+TGG - chr6_2:9990312-9990331 Msa0908920:CDS 60.0%
AGCCCAAGTTACGGCTGCTC+CGG + chr6_2:9990318-9990337 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 9990056 9990685 9990056 ID=Msa0908920;Name=Msa0908920
chr6_2 mRNA 9990056 9990685 9990056 ID=Msa0908920-mRNA-1;Parent=Msa0908920;Name=Msa0908920-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|209
chr6_2 exon 9990056 9990685 9990056 ID=Msa0908920-mRNA-1:exon:2524;Parent=Msa0908920-mRNA-1
chr6_2 CDS 9990056 9990685 9990056 ID=Msa0908920-mRNA-1:cds;Parent=Msa0908920-mRNA-1
Gene Sequence

>Msa0908920

ATGTTTGTATGGTGGCGCGATGTTCTACGTGAATCCACGTTGGAAGGACATCATACCAAAGTAGTACAATTAGGACCTCGATATGGTTCTATTCCGTTCATCGTATCGGAGGTTATGTTCCTTTTTGCTTTTTTTCGGGCTTCTTCTCATTCTTCTTTGGCACCTACGGTAGAGATCGGGGGTATTTGGCCCCCAAAAGGGATTTGGGTTTTAGATCCTCGGGAAATCCCATTTCTTAATACCCCTATTCTCCTTTCATCCGGAGCAGCCGTAACTTGGGCTCATCATGCTATACTCGCGGGGAAGGAAAAACGAGCAGTTTACGCTTTAGTAGCAACCGTTTCACTGGCTCTAGTATTCACTGGCTTTCAAGGAATGGAATATTATCAAGCACCCTTCACTATTTCGGATAGTATTTATGGTTCTACCTTTTTCTTAGCAACTGGCTTTCATGGTTTTCATGTGATTATAGGTACTCTTTTCTTGATCGTATGTGGTATTCGCCAATATCTTGGTCATCTGACCAAGGAGCATCACGTTGGCTTTGAAGCAGCTGCATGGTACTGGCATTTTGTAGACGTGGTTCGGTTATTCCCATTTGTCTCTATCTATTGGTGGGGAGGTATATGA

Protein sequence

>Msa0908920

MFVWWRDVLRESTLEGHHTKVVQLGPRYGSIPFIVSEVMFLFAFFRASSHSSLAPTVEIGGIWPPKGIWVLDPREIPFLNTPILLSSGAAVTWAHHAILAGKEKRAVYALVATVSLALVFTGFQGMEYYQAPFTISDSIYGSTFFLATGFHGFHVIIGTLFLIVCGIRQYLGHLTKEHHVGFEAAAWYWHFVDVVRLFPFVSIYWWGGI*