Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0983210 | RHN45450.1 | 97.744 | 133 | 3 | 0 | 1 | 133 | 49 | 181 | 8.27e-88 | 265 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0983210 | A0A396GWN7 | 97.744 | 133 | 3 | 0 | 1 | 133 | 49 | 181 | 3.95e-88 | 265 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0069500 | Msa0983210 | 0.824584 | 7.456511e-54 | -8.615850e-47 |
| Msa0697990 | Msa0983210 | 0.818814 | 1.610688e-52 | -8.615850e-47 |
| Msa0721280 | Msa0983210 | -0.819430 | 1.166351e-52 | -8.615850e-47 |
| Msa0983170 | Msa0983210 | 0.857959 | 1.143945e-62 | -8.615850e-47 |
| Msa0983180 | Msa0983210 | 0.868749 | 5.180990e-66 | -8.615850e-47 |
| Msa0983190 | Msa0983210 | 0.907183 | 6.668853e-81 | -8.615850e-47 |
| Msa0983210 | Msa0983220 | 0.846521 | 2.061491e-59 | -8.615850e-47 |
| Msa0983210 | Msa0983260 | 0.878914 | 1.909360e-69 | -8.615850e-47 |
| Msa0983210 | Msa0983300 | 0.852971 | 3.252883e-61 | -8.615850e-47 |
| Msa0983210 | Msa0983320 | 0.852162 | 5.532093e-61 | -8.615850e-47 |
| Msa0983210 | Msa0983350 | 0.857819 | 1.258732e-62 | -8.615850e-47 |
| Msa0983210 | Msa0983460 | 0.869970 | 2.076019e-66 | -8.615850e-47 |
| Msa0983210 | Msa0983490 | 0.855040 | 8.236881e-62 | -8.615850e-47 |
| Msa0983210 | Msa0983500 | 0.976135 | 2.991318e-141 | -8.615850e-47 |
| Msa0983210 | Msa0983520 | 0.893352 | 6.812258e-75 | -8.615850e-47 |
| Msa0983210 | Msa0983530 | 0.859971 | 2.859216e-63 | -8.615850e-47 |
| Msa0983210 | Msa0983540 | 0.855899 | 4.629088e-62 | -8.615850e-47 |
| Msa0983210 | Msa1228620 | 0.842094 | 3.189074e-58 | -8.615850e-47 |
| Msa0983210 | Msa1298510 | -0.800558 | 1.362206e-48 | -8.615850e-47 |
| Msa0983210 | Msa1357890 | -0.821117 | 4.788171e-53 | -8.615850e-47 |
| Msa0983210 | Msa1391970 | 0.854958 | 8.701672e-62 | -8.615850e-47 |
| Msa0983210 | Msa1392010 | 0.847938 | 8.423402e-60 | -8.615850e-47 |
| Msa0983210 | Msa1392030 | 0.846656 | 1.893742e-59 | -8.615850e-47 |
| Msa0983210 | Msa1392120 | 0.860503 | 1.973805e-63 | -8.615850e-47 |
| Msa0983210 | Msa1392160 | 0.897181 | 1.803558e-76 | -8.615850e-47 |
| Msa0983210 | Msa1398090 | -0.810646 | 1.039365e-50 | -8.615850e-47 |
| Msa0983210 | Msa1466540 | -0.809257 | 2.069236e-50 | -8.615850e-47 |
| Msa0261720 | Msa0983210 | -0.802942 | 4.415277e-49 | -8.615850e-47 |
| Msa0908830 | Msa0983210 | 0.853325 | 2.575444e-61 | -8.615850e-47 |
| Msa0908840 | Msa0983210 | 0.868262 | 7.443482e-66 | -8.615850e-47 |
| Msa0908850 | Msa0983210 | 0.893477 | 6.061749e-75 | -8.615850e-47 |
| Msa0908870 | Msa0983210 | 0.960461 | 1.388971e-118 | -8.615850e-47 |
| Msa0908920 | Msa0983210 | 0.839455 | 1.568546e-57 | -8.615850e-47 |
| Msa0908930 | Msa0983210 | 0.865935 | 4.115330e-65 | -8.615850e-47 |
| Msa0920000 | Msa0983210 | 0.807233 | 5.586856e-50 | -8.615850e-47 |
| Msa0920060 | Msa0983210 | 0.833541 | 5.025690e-56 | -8.615850e-47 |
| Msa0944900 | Msa0983210 | 0.962231 | 1.243611e-120 | -8.615850e-47 |
| Msa0944920 | Msa0983210 | 0.900072 | 1.055290e-77 | -8.615850e-47 |
| Msa0944930 | Msa0983210 | 0.860189 | 2.456577e-63 | -8.615850e-47 |
| Msa0944940 | Msa0983210 | 0.864717 | 9.943761e-65 | -8.615850e-47 |
| Msa0944990 | Msa0983210 | 0.876471 | 1.360039e-68 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0983210 | MtrunA17_MTg0491251 | 98.496 | 133 | 2 | 0 | 1 | 133 | 81 | 213 | 1.16e-92 | 267 |
| Msa0983210 | MtrunA17_Chr7g0231341 | 97.744 | 133 | 3 | 0 | 1 | 133 | 49 | 181 | 7.61e-92 | 265 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCCGACGCCGACCCATTT+TGG | 0.210676 | 6_4:+26319769 | None:intergenic |
| CTCTTTGGATGTATCCTTCT+GGG | 0.227156 | 6_4:-26319869 | None:intergenic |
| CCTCTTTGGATGTATCCTTC+TGG | 0.235353 | 6_4:-26319870 | None:intergenic |
| AGACTTCGTTGCAAATAAAT+TGG | 0.238736 | 6_4:-26319649 | Msa0983210:CDS |
| CCCGCCGCTCCTATCCAAAA+TGG | 0.239934 | 6_4:-26319783 | Msa0983210:CDS |
| CGTAGCGCTTGCTCATAAAT+TGG | 0.318064 | 6_4:+26319603 | None:intergenic |
| ATTGGACCCTGCGGCTGCCC+CGG | 0.373068 | 6_4:+26319621 | None:intergenic |
| GATAGGAGCGGCGGGTCCGC+CGG | 0.375170 | 6_4:+26319791 | None:intergenic |
| TATCCTTCTGGGGCTGATGC+TGG | 0.380202 | 6_4:-26319858 | Msa0983210:CDS |
| AGCGGCGGGTCCGCCGGGGC+GGG | 0.390016 | 6_4:+26319797 | None:intergenic |
| TATGGTTTCGCGGCCGGAGC+TGG | 0.394590 | 6_4:+26319726 | None:intergenic |
| GGGAAGGCTGATGCTGCTCT+TGG | 0.402499 | 6_4:+26319817 | None:intergenic |
| GCGGGTCCGCCGGGGCGGGA+AGG | 0.403262 | 6_4:+26319801 | None:intergenic |
| TTGGACCCTGCGGCTGCCCC+GGG | 0.438206 | 6_4:+26319622 | None:intergenic |
| GGAAGACCCGGGGCAGCCGC+AGG | 0.439885 | 6_4:-26319628 | Msa0983210:CDS |
| TAGGAGCGGCGGGTCCGCCG+GGG | 0.472146 | 6_4:+26319793 | None:intergenic |
| TTCCGTGGAGCAGCCAGCTC+CGG | 0.472407 | 6_4:-26319739 | Msa0983210:CDS |
| GGCCGGAGCTGGCTGCTCCA+CGG | 0.474217 | 6_4:+26319737 | None:intergenic |
| TGCAAATAAATTGGAAGACC+CGG | 0.488016 | 6_4:-26319640 | Msa0983210:CDS |
| CAGCATCAGCCTTCCCGCCC+CGG | 0.489074 | 6_4:-26319810 | Msa0983210:CDS |
| CCGCCGCTCCTATCCAAAAT+GGG | 0.493631 | 6_4:-26319782 | Msa0983210:CDS |
| GGACGAACTTCAGCGCTGGC+TGG | 0.506906 | 6_4:-26319538 | Msa0983210:CDS |
| AGCGCTGGCTGGTTTCACTA+AGG | 0.509513 | 6_4:-26319527 | Msa0983210:CDS |
| AGCTGAAGGAGGGCTATGTA+TGG | 0.518841 | 6_4:+26319708 | None:intergenic |
| GCGCTGGCTGGTTTCACTAA+GGG | 0.521276 | 6_4:-26319526 | Msa0983210:CDS |
| GCTATGTATGGTTTCGCGGC+CGG | 0.522221 | 6_4:+26319720 | None:intergenic |
| GAGGGCTATGTATGGTTTCG+CGG | 0.534222 | 6_4:+26319716 | None:intergenic |
| AGCAAGCGCTACGCCTGGTA+TGG | 0.535586 | 6_4:-26319593 | Msa0983210:CDS |
| TTATGAGCAAGCGCTACGCC+TGG | 0.557111 | 6_4:-26319598 | Msa0983210:CDS |
| CGCTCCTATCCAAAATGGGT+CGG | 0.568858 | 6_4:-26319778 | Msa0983210:CDS |
| GATCGATGGCAGTACTAACC+AGG | 0.569954 | 6_4:-26319559 | Msa0983210:CDS |
| GCAAATAAATTGGAAGACCC+GGG | 0.573068 | 6_4:-26319639 | Msa0983210:CDS |
| ATAGCCCTCCTTCAGCTAGA+GGG | 0.580354 | 6_4:-26319702 | Msa0983210:CDS |
| ACCAGGACGAACTTCAGCGC+TGG | 0.584629 | 6_4:-26319542 | Msa0983210:CDS |
| GAAGACCCGGGGCAGCCGCA+GGG | 0.585751 | 6_4:-26319627 | Msa0983210:CDS |
| GCCAGCGCTGAAGTTCGTCC+TGG | 0.586980 | 6_4:+26319541 | None:intergenic |
| TCTTTGGATGTATCCTTCTG+GGG | 0.587601 | 6_4:-26319868 | None:intergenic |
| TGGCTGCTCCACGGAACTAG+TGG | 0.588921 | 6_4:+26319746 | None:intergenic |
| AAAATGGATTTGTATTGTCG+GGG | 0.604607 | 6_4:+26319498 | None:intergenic |
| TCTCGAGTTCATACCATACC+AGG | 0.605945 | 6_4:+26319580 | None:intergenic |
| TGGTATGAACTCGAGATCGA+TGG | 0.622194 | 6_4:-26319573 | Msa0983210:CDS |
| TAGCCCTCCTTCAGCTAGAG+GGG | 0.628752 | 6_4:-26319701 | Msa0983210:CDS |
| TATCCAAAATGGGTCGGCGT+CGG | 0.631571 | 6_4:-26319772 | Msa0983210:CDS |
| GAGCCAGCATCAGCCCCAGA+AGG | 0.632838 | 6_4:+26319855 | None:intergenic |
| TGCTCATAAATTGGACCCTG+CGG | 0.637696 | 6_4:+26319612 | None:intergenic |
| CATAGCCCTCCTTCAGCTAG+AGG | 0.642446 | 6_4:-26319703 | Msa0983210:CDS |
| AGCCCTCCTTCAGCTAGAGG+GGG | 0.644919 | 6_4:-26319700 | Msa0983210:CDS |
| AAATGGATTTGTATTGTCGG+GGG | 0.651927 | 6_4:+26319499 | None:intergenic |
| CAAATAAATTGGAAGACCCG+GGG | 0.684347 | 6_4:-26319638 | Msa0983210:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATGGATTTGTATTGTC+GGG | + | chr6_4:26319837-26319856 | None:intergenic | 25.0% |
| !! | CAAAAATGGATTTGTATTGT+CGG | + | chr6_4:26319838-26319857 | None:intergenic | 25.0% |
| AGACTTCGTTGCAAATAAAT+TGG | - | chr6_4:26319682-26319701 | Msa0983210:CDS | 30.0% | |
| ! | AAAATGGATTTGTATTGTCG+GGG | + | chr6_4:26319836-26319855 | None:intergenic | 30.0% |
| ! | CGACAATACAAATCCATTTT+TGG | - | chr6_4:26319836-26319855 | Msa0983210:CDS | 30.0% |
| TGCAAATAAATTGGAAGACC+CGG | - | chr6_4:26319691-26319710 | Msa0983210:CDS | 35.0% | |
| ! | AAATGGATTTGTATTGTCGG+GGG | + | chr6_4:26319835-26319854 | None:intergenic | 35.0% |
| GCAAATAAATTGGAAGACCC+GGG | - | chr6_4:26319692-26319711 | Msa0983210:CDS | 40.0% | |
| CAAATAAATTGGAAGACCCG+GGG | - | chr6_4:26319693-26319712 | Msa0983210:CDS | 40.0% | |
| !!! | AATCCATTTTTGGCTTCTCC+CGG | - | chr6_4:26319846-26319865 | Msa0983210:CDS | 40.0% |
| TGCTCATAAATTGGACCCTG+CGG | + | chr6_4:26319722-26319741 | None:intergenic | 45.0% | |
| CGTAGCGCTTGCTCATAAAT+TGG | + | chr6_4:26319731-26319750 | None:intergenic | 45.0% | |
| TCTCGAGTTCATACCATACC+AGG | + | chr6_4:26319754-26319773 | None:intergenic | 45.0% | |
| TGGTATGAACTCGAGATCGA+TGG | - | chr6_4:26319758-26319777 | Msa0983210:CDS | 45.0% | |
| !!! | ATCCATTTTTGGCTTCTCCC+GGG | - | chr6_4:26319847-26319866 | Msa0983210:CDS | 45.0% |
| ! | CGACCCATTTTGGATAGGAG+CGG | + | chr6_4:26319555-26319574 | None:intergenic | 50.0% |
| CGCTCCTATCCAAAATGGGT+CGG | - | chr6_4:26319553-26319572 | Msa0983210:CDS | 50.0% | |
| TATCCAAAATGGGTCGGCGT+CGG | - | chr6_4:26319559-26319578 | Msa0983210:CDS | 50.0% | |
| GAGGGCTATGTATGGTTTCG+CGG | + | chr6_4:26319618-26319637 | None:intergenic | 50.0% | |
| AGCTGAAGGAGGGCTATGTA+TGG | + | chr6_4:26319626-26319645 | None:intergenic | 50.0% | |
| ATAGCCCTCCTTCAGCTAGA+GGG | - | chr6_4:26319629-26319648 | Msa0983210:CDS | 50.0% | |
| GATCGATGGCAGTACTAACC+AGG | - | chr6_4:26319772-26319791 | Msa0983210:CDS | 50.0% | |
| !!! | TCCATTTTTGGCTTCTCCCG+GGG | - | chr6_4:26319848-26319867 | Msa0983210:CDS | 50.0% |
| !! | TATCCTTCTGGGGCTGATGC+TGG | - | chr6_4:26319473-26319492 | Msa0983210:CDS | 55.0% |
| ! | CCATTTTGGATAGGAGCGGC+GGG | + | chr6_4:26319551-26319570 | None:intergenic | 55.0% |
| ! | CCCATTTTGGATAGGAGCGG+CGG | + | chr6_4:26319552-26319571 | None:intergenic | 55.0% |
| CCGCCGCTCCTATCCAAAAT+GGG | - | chr6_4:26319549-26319568 | Msa0983210:CDS | 55.0% | |
| ! | GACGCCGACCCATTTTGGAT+AGG | + | chr6_4:26319560-26319579 | None:intergenic | 55.0% |
| GCTATGTATGGTTTCGCGGC+CGG | + | chr6_4:26319614-26319633 | None:intergenic | 55.0% | |
| CATAGCCCTCCTTCAGCTAG+AGG | - | chr6_4:26319628-26319647 | Msa0983210:CDS | 55.0% | |
| TAGCCCTCCTTCAGCTAGAG+GGG | - | chr6_4:26319630-26319649 | Msa0983210:CDS | 55.0% | |
| TTATGAGCAAGCGCTACGCC+TGG | - | chr6_4:26319733-26319752 | Msa0983210:CDS | 55.0% | |
| !! | AGCGCTGGCTGGTTTCACTA+AGG | - | chr6_4:26319804-26319823 | Msa0983210:CDS | 55.0% |
| !! | GCGCTGGCTGGTTTCACTAA+GGG | - | chr6_4:26319805-26319824 | Msa0983210:CDS | 55.0% |
| !!! | CCATTTTTGGCTTCTCCCGG+GGG | - | chr6_4:26319849-26319868 | Msa0983210:CDS | 55.0% |
| !!! | CATTTTTGGCTTCTCCCGGG+GGG | - | chr6_4:26319850-26319869 | Msa0983210:CDS | 55.0% |
| GGGAAGGCTGATGCTGCTCT+TGG | + | chr6_4:26319517-26319536 | None:intergenic | 60.0% | |
| CCCGCCGCTCCTATCCAAAA+TGG | - | chr6_4:26319548-26319567 | Msa0983210:CDS | 60.0% | |
| ! | AAGCCGACGCCGACCCATTT+TGG | + | chr6_4:26319565-26319584 | None:intergenic | 60.0% |
| ! | GTCGGCTTCCACTAGTTCCG+TGG | - | chr6_4:26319577-26319596 | Msa0983210:CDS | 60.0% |
| TGGCTGCTCCACGGAACTAG+TGG | + | chr6_4:26319588-26319607 | None:intergenic | 60.0% | |
| AGCCCTCCTTCAGCTAGAGG+GGG | - | chr6_4:26319631-26319650 | Msa0983210:CDS | 60.0% | |
| CTCCCCCTCTAGCTGAAGGA+GGG | + | chr6_4:26319636-26319655 | None:intergenic | 60.0% | |
| TCCTTCAGCTAGAGGGGGAG+CGG | - | chr6_4:26319636-26319655 | Msa0983210:CDS | 60.0% | |
| AGCAAGCGCTACGCCTGGTA+TGG | - | chr6_4:26319738-26319757 | Msa0983210:CDS | 60.0% | |
| ACCAGGACGAACTTCAGCGC+TGG | - | chr6_4:26319789-26319808 | Msa0983210:CDS | 60.0% | |
| CCCCCGGGAGAAGCCAAAAA+TGG | + | chr6_4:26319852-26319871 | None:intergenic | 60.0% | |
| GAGCCAGCATCAGCCCCAGA+AGG | + | chr6_4:26319479-26319498 | None:intergenic | 65.0% | |
| TTCCGTGGAGCAGCCAGCTC+CGG | - | chr6_4:26319592-26319611 | Msa0983210:CDS | 65.0% | |
| TATGGTTTCGCGGCCGGAGC+TGG | + | chr6_4:26319608-26319627 | None:intergenic | 65.0% | |
| GCTCCCCCTCTAGCTGAAGG+AGG | + | chr6_4:26319637-26319656 | None:intergenic | 65.0% | |
| TCCGCTCCCCCTCTAGCTGA+AGG | + | chr6_4:26319640-26319659 | None:intergenic | 65.0% | |
| ! | GCCAGCGCTGAAGTTCGTCC+TGG | + | chr6_4:26319793-26319812 | None:intergenic | 65.0% |
| !! | GGACGAACTTCAGCGCTGGC+TGG | - | chr6_4:26319793-26319812 | Msa0983210:CDS | 65.0% |
| CAGCATCAGCCTTCCCGCCC+CGG | - | chr6_4:26319521-26319540 | Msa0983210:CDS | 70.0% | |
| ATTGGACCCTGCGGCTGCCC+CGG | + | chr6_4:26319713-26319732 | None:intergenic | 70.0% | |
| CATCAGCCTTCCCGCCCCGG+CGG | - | chr6_4:26319524-26319543 | Msa0983210:CDS | 75.0% | |
| ATAGGAGCGGCGGGTCCGCC+GGG | + | chr6_4:26319542-26319561 | None:intergenic | 75.0% | |
| GATAGGAGCGGCGGGTCCGC+CGG | + | chr6_4:26319543-26319562 | None:intergenic | 75.0% | |
| GGCCGGAGCTGGCTGCTCCA+CGG | + | chr6_4:26319597-26319616 | None:intergenic | 75.0% | |
| GAAGACCCGGGGCAGCCGCA+GGG | - | chr6_4:26319704-26319723 | Msa0983210:CDS | 75.0% | |
| TTGGACCCTGCGGCTGCCCC+GGG | + | chr6_4:26319712-26319731 | None:intergenic | 75.0% | |
| ! | TAGGAGCGGCGGGTCCGCCG+GGG | + | chr6_4:26319541-26319560 | None:intergenic | 80.0% |
| ! | GGAAGACCCGGGGCAGCCGC+AGG | - | chr6_4:26319703-26319722 | Msa0983210:CDS | 80.0% |
| ! | GCGGGTCCGCCGGGGCGGGA+AGG | + | chr6_4:26319533-26319552 | None:intergenic | 90.0% |
| ! | AGCGGCGGGTCCGCCGGGGC+GGG | + | chr6_4:26319537-26319556 | None:intergenic | 90.0% |
| ! | GAGCGGCGGGTCCGCCGGGG+CGG | + | chr6_4:26319538-26319557 | None:intergenic | 90.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 26319470 | 26319883 | 26319470 | ID=Msa0983210;Name=Msa0983210 |
| chr6_4 | mRNA | 26319470 | 26319883 | 26319470 | ID=Msa0983210-mRNA-1;Parent=Msa0983210;Name=Msa0983210-mRNA-1;_AED=0.26;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|137 |
| chr6_4 | exon | 26319470 | 26319883 | 26319470 | ID=Msa0983210-mRNA-1:exon:7383;Parent=Msa0983210-mRNA-1 |
| chr6_4 | CDS | 26319470 | 26319883 | 26319470 | ID=Msa0983210-mRNA-1:cds;Parent=Msa0983210-mRNA-1 |
| Gene Sequence |
| Protein sequence |
