AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0983180


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 40 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAAAAGAAAGTAGGTTTC+GGG 0.299621 6_4:+26312157 Msa0983180:intron
TGCGCGAGTCACCTTCTTCT+AGG 0.325482 6_4:+26312334 Msa0983180:CDS
ACCAGCCAGCTGGGTTGGTT+TGG 0.332153 6_4:+26312275 Msa0983180:CDS
CGCAATGGCTTTCTCTGATA+GGG 0.371132 6_4:+26311869 None:intergenic
ATTCCTTTCTATTGAAAAGA+AGG 0.387353 6_4:+26312300 Msa0983180:CDS
GCGCAATGGCTTTCTCTGAT+AGG 0.391358 6_4:+26311868 None:intergenic
GTCGTCCGTCTCAGGTTTGC+CGG 0.398406 6_4:+26312231 Msa0983180:CDS
GGACCGTGGTCGTCCGTCTC+AGG 0.441663 6_4:+26312223 Msa0983180:CDS
AGCAGCACCTTGTATAGGTT+GGG 0.447543 6_4:-26311912 None:intergenic
GTACACCTCCGGTGCTGATA+AGG 0.450222 6_4:+26312197 Msa0983180:CDS
GATTGACCAGCCAGCTGGGT+TGG 0.459599 6_4:+26312270 Msa0983180:CDS
GTCAATCTCAGAGATCTTAT+CGG 0.462954 6_4:-26312254 None:intergenic
AAGCGAGCAGCACCTTGTAT+AGG 0.473472 6_4:-26311917 None:intergenic
TCTGAGATTGACCAGCCAGC+TGG 0.486873 6_4:+26312265 Msa0983180:CDS
TTCATTGATTAGTACACCTC+CGG 0.492064 6_4:+26312186 Msa0983180:CDS
CGCTTTCTCTCAGCCACCAG+TGG 0.505851 6_4:+26311935 Msa0983180:CDS
CTGAGATTGACCAGCCAGCT+GGG 0.514570 6_4:+26312266 Msa0983180:CDS
CACCTTCTTCTAGGCTCCGC+TGG 0.516734 6_4:+26312343 Msa0983180:CDS
GCCAAACCAACCCAGCTGGC+TGG 0.523772 6_4:-26312276 None:intergenic
GAGCAGCACCTTGTATAGGT+TGG 0.534433 6_4:-26311913 None:intergenic
ATCTCAGAGATCTTATCGGC+CGG 0.550066 6_4:-26312250 None:intergenic
AATAGCCAAACCAACCCAGC+TGG 0.553661 6_4:-26312280 None:intergenic
CCTCCGGTGCTGATAAGGTC+GGG 0.561319 6_4:+26312202 Msa0983180:CDS
ATCGGCCGGCAAACCTGAGA+CGG 0.579939 6_4:-26312236 None:intergenic
GCCACCAGTGGCTTATGTAG+TGG 0.581920 6_4:+26311947 Msa0983180:CDS
TGCTGATAAGGTCGGGACCG+TGG 0.590314 6_4:+26312209 Msa0983180:CDS
GTCCAGCGGAGCCTAGAAGA+AGG 0.604022 6_4:-26312345 None:intergenic
CTAGGCTCCGCTGGACGACA+CGG 0.612977 6_4:+26312352 Msa0983180:CDS
GAGAATGCCGTGTCGTCCAG+CGG 0.617479 6_4:-26312359 None:intergenic
CCCGACCTTATCAGCACCGG+AGG 0.624401 6_4:-26312202 None:intergenic
AGCACCTTGTATAGGTTGGG+CGG 0.628644 6_4:-26311909 None:intergenic
AGCTCCGCCCAACCTATACA+AGG 0.631063 6_4:+26311905 Msa0983180:CDS
CCGACCACTACATAAGCCAC+TGG 0.631161 6_4:-26311951 None:intergenic
ACCTCCGGTGCTGATAAGGT+CGG 0.637828 6_4:+26312201 Msa0983180:CDS
GCAATGGCTTTCTCTGATAG+GGG 0.641776 6_4:+26311870 None:intergenic
GGTCCCGACCTTATCAGCAC+CGG 0.654281 6_4:-26312205 None:intergenic
AAACCTGAGACGGACGACCA+CGG 0.659367 6_4:-26312226 None:intergenic
ACCACTACATAAGCCACTGG+TGG 0.667213 6_4:-26311948 None:intergenic
AGAAAAGAAAGTAGGTTTCG+GGG 0.671452 6_4:+26312158 Msa0983180:intron
GAAAAGAAAGTAGGTTTCGG+GGG 0.676234 6_4:+26312159 Msa0983180:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTTGTCAGAAAAGAAAGT+AGG + chr6_4:26312150-26312169 Msa0983180:intron 25.0%
! ATTCCTTTCTATTGAAAAGA+AGG + chr6_4:26312300-26312319 Msa0983180:CDS 25.0%
TCAGAAAAGAAAGTAGGTTT+CGG + chr6_4:26312156-26312175 Msa0983180:intron 30.0%
! ACTCCTTCTTTTCAATAGAA+AGG - chr6_4:26312306-26312325 None:intergenic 30.0%
ACCTTTGGATTCTTAAGTAG+GGG - chr6_4:26312000-26312019 None:intergenic 35.0%
GACCTTTGGATTCTTAAGTA+GGG - chr6_4:26312001-26312020 None:intergenic 35.0%
TGACCTTTGGATTCTTAAGT+AGG - chr6_4:26312002-26312021 None:intergenic 35.0%
GTTGTTGTGATTGAGTTTGA+GGG - chr6_4:26312104-26312123 None:intergenic 35.0%
TGTTGTTGTGATTGAGTTTG+AGG - chr6_4:26312105-26312124 None:intergenic 35.0%
CAGAAAAGAAAGTAGGTTTC+GGG + chr6_4:26312157-26312176 Msa0983180:intron 35.0%
AGAAAAGAAAGTAGGTTTCG+GGG + chr6_4:26312158-26312177 Msa0983180:intron 35.0%
! TTCATTGATTAGTACACCTC+CGG + chr6_4:26312186-26312205 Msa0983180:CDS 35.0%
GTCAATCTCAGAGATCTTAT+CGG - chr6_4:26312257-26312276 None:intergenic 35.0%
CCTTTGGATTCTTAAGTAGG+GGG - chr6_4:26311999-26312018 None:intergenic 40.0%
AAGAATCCAAAGGTCACCTT+TGG + chr6_4:26312006-26312025 Msa0983180:intron 40.0%
GAAAAGAAAGTAGGTTTCGG+GGG + chr6_4:26312159-26312178 Msa0983180:intron 40.0%
AGCAGCACCTTGTATAGGTT+GGG - chr6_4:26311915-26311934 None:intergenic 45.0%
TTCTTAAGTAGGGGGCAAAG+AGG - chr6_4:26311991-26312010 None:intergenic 45.0%
CCCCCTACTTAAGAATCCAA+AGG + chr6_4:26311996-26312015 Msa0983180:intron 45.0%
GCTTTGGTTACGAAAGTAGC+CGG + chr6_4:26312042-26312061 Msa0983180:intron 45.0%
CTTTGGTTACGAAAGTAGCC+GGG + chr6_4:26312043-26312062 Msa0983180:intron 45.0%
TTTGGTTACGAAAGTAGCCG+GGG + chr6_4:26312044-26312063 Msa0983180:intron 45.0%
TCACAACAACATGTCGTGAC+CGG + chr6_4:26312114-26312133 Msa0983180:intron 45.0%
CAAAATCCACCAAGCCTAAC+CGG - chr6_4:26312136-26312155 None:intergenic 45.0%
ATCTCAGAGATCTTATCGGC+CGG - chr6_4:26312253-26312272 None:intergenic 45.0%
! AGCACCTTGTATAGGTTGGG+CGG - chr6_4:26311912-26311931 None:intergenic 50.0%
GAGCAGCACCTTGTATAGGT+TGG - chr6_4:26311916-26311935 None:intergenic 50.0%
AAGCGAGCAGCACCTTGTAT+AGG - chr6_4:26311920-26311939 None:intergenic 50.0%
ACCACTACATAAGCCACTGG+TGG - chr6_4:26311951-26311970 None:intergenic 50.0%
CCAGTGGCTTATGTAGTGGT+CGG + chr6_4:26311951-26311970 Msa0983180:intron 50.0%
CAACAGCCAAAGGTGACCTT+TGG - chr6_4:26312015-26312034 None:intergenic 50.0%
AGCGTCAAGACAACAGCCAA+AGG - chr6_4:26312025-26312044 None:intergenic 50.0%
TGGCTGTTGTCTTGACGCTT+TGG + chr6_4:26312026-26312045 Msa0983180:intron 50.0%
CCATCCATAAACCTAGACCC+CGG - chr6_4:26312064-26312083 None:intergenic 50.0%
ACAACATGTCGTGACCGGTT+AGG + chr6_4:26312119-26312138 Msa0983180:intron 50.0%
AAGAAAGTAGGTTTCGGGGG+TGG + chr6_4:26312162-26312181 Msa0983180:intron 50.0%
AGAAAGTAGGTTTCGGGGGT+GGG + chr6_4:26312163-26312182 Msa0983180:intron 50.0%
AATAGCCAAACCAACCCAGC+TGG - chr6_4:26312283-26312302 None:intergenic 50.0%
AGCTCCGCCCAACCTATACA+AGG + chr6_4:26311905-26311924 Msa0983180:CDS 55.0%
GCCACCAGTGGCTTATGTAG+TGG + chr6_4:26311947-26311966 Msa0983180:CDS 55.0%
CCGACCACTACATAAGCCAC+TGG - chr6_4:26311954-26311973 None:intergenic 55.0%
TACGAAAGTAGCCGGGGTCT+AGG + chr6_4:26312050-26312069 Msa0983180:intron 55.0%
GTAGCCGGGGTCTAGGTTTA+TGG + chr6_4:26312057-26312076 Msa0983180:intron 55.0%
CCGGGGTCTAGGTTTATGGA+TGG + chr6_4:26312061-26312080 Msa0983180:intron 55.0%
ATGTCGTGACCGGTTAGGCT+TGG + chr6_4:26312124-26312143 Msa0983180:intron 55.0%
!! GTACACCTCCGGTGCTGATA+AGG + chr6_4:26312197-26312216 Msa0983180:CDS 55.0%
!! ACCTCCGGTGCTGATAAGGT+CGG + chr6_4:26312201-26312220 Msa0983180:CDS 55.0%
AAACCTGAGACGGACGACCA+CGG - chr6_4:26312229-26312248 None:intergenic 55.0%
! TCTGAGATTGACCAGCCAGC+TGG + chr6_4:26312265-26312284 Msa0983180:CDS 55.0%
CTGAGATTGACCAGCCAGCT+GGG + chr6_4:26312266-26312285 Msa0983180:CDS 55.0%
TGCGCGAGTCACCTTCTTCT+AGG + chr6_4:26312334-26312353 Msa0983180:CDS 55.0%
CGCTTTCTCTCAGCCACCAG+TGG + chr6_4:26311935-26311954 Msa0983180:CDS 60.0%
TCGTGACCGGTTAGGCTTGG+TGG + chr6_4:26312127-26312146 Msa0983180:intron 60.0%
!! CCTCCGGTGCTGATAAGGTC+GGG + chr6_4:26312202-26312221 Msa0983180:CDS 60.0%
GGTCCCGACCTTATCAGCAC+CGG - chr6_4:26312208-26312227 None:intergenic 60.0%
TGCTGATAAGGTCGGGACCG+TGG + chr6_4:26312209-26312228 Msa0983180:CDS 60.0%
GTCGTCCGTCTCAGGTTTGC+CGG + chr6_4:26312231-26312250 Msa0983180:CDS 60.0%
ATCGGCCGGCAAACCTGAGA+CGG - chr6_4:26312239-26312258 None:intergenic 60.0%
GATTGACCAGCCAGCTGGGT+TGG + chr6_4:26312270-26312289 Msa0983180:CDS 60.0%
!! ACCAGCCAGCTGGGTTGGTT+TGG + chr6_4:26312275-26312294 Msa0983180:CDS 60.0%
CACCTTCTTCTAGGCTCCGC+TGG + chr6_4:26312343-26312362 Msa0983180:CDS 60.0%
GTCCAGCGGAGCCTAGAAGA+AGG - chr6_4:26312348-26312367 None:intergenic 60.0%
GAGAATGCCGTGTCGTCCAG+CGG - chr6_4:26312362-26312381 None:intergenic 60.0%
! GGGGGCAAAGAGGAGCACGT+AGG - chr6_4:26311981-26312000 None:intergenic 65.0%
! CCCGACCTTATCAGCACCGG+AGG - chr6_4:26312205-26312224 None:intergenic 65.0%
GCCAAACCAACCCAGCTGGC+TGG - chr6_4:26312279-26312298 None:intergenic 65.0%
CTAGGCTCCGCTGGACGACA+CGG + chr6_4:26312352-26312371 Msa0983180:CDS 65.0%
GGACCGTGGTCGTCCGTCTC+AGG + chr6_4:26312223-26312242 Msa0983180:CDS 70.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 26311873 26312387 26311873 ID=Msa0983180;Name=Msa0983180
chr6_4 mRNA 26311873 26312387 26311873 ID=Msa0983180-mRNA-1;Parent=Msa0983180;Name=Msa0983180-mRNA-1;_AED=0.27;_eAED=0.39;_QI=0|0|0|1|0|0|2|0|103
chr6_4 exon 26311873 26311968 26311873 ID=Msa0983180-mRNA-1:exon:7410;Parent=Msa0983180-mRNA-1
chr6_4 exon 26312172 26312387 26312172 ID=Msa0983180-mRNA-1:exon:7411;Parent=Msa0983180-mRNA-1
chr6_4 CDS 26311873 26311968 26311873 ID=Msa0983180-mRNA-1:cds;Parent=Msa0983180-mRNA-1
chr6_4 CDS 26312172 26312387 26312172 ID=Msa0983180-mRNA-1:cds;Parent=Msa0983180-mRNA-1
Gene Sequence

>Msa0983180

ATGGCTTTCTCTGATAGGGGCTCGCTTCTTCAAGCTCCGCCCAACCTATACAAGGTGCTGCTCGCTTTCTCTCAGCCACCAGTGGCTTATGTAGTGGTTTCGGGGGTGGGTTCATTGATTAGTACACCTCCGGTGCTGATAAGGTCGGGACCGTGGTCGTCCGTCTCAGGTTTGCCGGCCGATAAGATCTCTGAGATTGACCAGCCAGCTGGGTTGGTTTGGCTATTCCTTTCTATTGAAAAGAAGGAGTCAGCTGTCTGCGCGAGTCACCTTCTTCTAGGCTCCGCTGGACGACACGGCATTCTCGTTTAA

Protein sequence

>Msa0983180

MAFSDRGSLLQAPPNLYKVLLAFSQPPVAYVVVSGVGSLISTPPVLIRSGPWSSVSGLPADKISEIDQPAGLVWLFLSIEKKESAVCASHLLLGSAGRHGILV*