AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0983530


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 40 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAAAAGAAAGTAGGTTTC+GGG 0.299621 6_4:-26627572 Msa0983530:intron
TGCGCGAGTCACCTTCTTCT+AGG 0.325482 6_4:-26627395 Msa0983530:CDS
ACCAGCCAGCTGGGTTGGTT+TGG 0.332153 6_4:-26627454 Msa0983530:CDS
CGCAATGGCTTTCTCTGATA+GGG 0.371132 6_4:-26627860 None:intergenic
ATTCCTTTCTATTGAAAAGA+AGG 0.387353 6_4:-26627429 Msa0983530:CDS
GCGCAATGGCTTTCTCTGAT+AGG 0.391358 6_4:-26627861 None:intergenic
GTCGTCCGTCTCAGGTTTGC+CGG 0.398406 6_4:-26627498 Msa0983530:CDS
GGACCGTGGTCGTCCGTCTC+AGG 0.441663 6_4:-26627506 Msa0983530:CDS
AGCAGCACCTTGTATAGGTT+GGG 0.447543 6_4:+26627817 None:intergenic
GTACACCTCCGGTGCTGATA+AGG 0.450222 6_4:-26627532 Msa0983530:CDS
GATTGACCAGCCAGCTGGGT+TGG 0.459599 6_4:-26627459 Msa0983530:CDS
GTCAATCTCAGAGATCTTAT+CGG 0.462954 6_4:+26627475 None:intergenic
AAGCGAGCAGCACCTTGTAT+AGG 0.473472 6_4:+26627812 None:intergenic
TCTGAGATTGACCAGCCAGC+TGG 0.486873 6_4:-26627464 Msa0983530:CDS
TTCATTGATTAGTACACCTC+CGG 0.492064 6_4:-26627543 Msa0983530:CDS
CGCTTTCTCTCAGCCACCAG+TGG 0.505851 6_4:-26627794 Msa0983530:CDS
CTGAGATTGACCAGCCAGCT+GGG 0.514570 6_4:-26627463 Msa0983530:CDS
CACCTTCTTCTAGGCTCCGC+TGG 0.516734 6_4:-26627386 Msa0983530:CDS
GCCAAACCAACCCAGCTGGC+TGG 0.523772 6_4:+26627453 None:intergenic
GAGCAGCACCTTGTATAGGT+TGG 0.534433 6_4:+26627816 None:intergenic
ATCTCAGAGATCTTATCGGC+CGG 0.550066 6_4:+26627479 None:intergenic
AATAGCCAAACCAACCCAGC+TGG 0.553661 6_4:+26627449 None:intergenic
CCTCCGGTGCTGATAAGGTC+GGG 0.561319 6_4:-26627527 Msa0983530:CDS
ATCGGCCGGCAAACCTGAGA+CGG 0.579939 6_4:+26627493 None:intergenic
GCCACCAGTGGCTTATGTAG+TGG 0.581920 6_4:-26627782 Msa0983530:intron
TGCTGATAAGGTCGGGACCG+TGG 0.590314 6_4:-26627520 Msa0983530:CDS
GTCCAGCGGAGCCTAGAAGA+AGG 0.604022 6_4:+26627384 None:intergenic
CTAGGCTCCGCTGGACGACA+CGG 0.612977 6_4:-26627377 Msa0983530:CDS
GAGAATGCCGTGTCGTCCAG+CGG 0.617479 6_4:+26627370 None:intergenic
CCCGACCTTATCAGCACCGG+AGG 0.624401 6_4:+26627527 None:intergenic
AGCACCTTGTATAGGTTGGG+CGG 0.628644 6_4:+26627820 None:intergenic
AGCTCCGCCCAACCTATACA+AGG 0.631063 6_4:-26627824 Msa0983530:CDS
CCGACCACTACATAAGCCAC+TGG 0.631161 6_4:+26627778 None:intergenic
ACCTCCGGTGCTGATAAGGT+CGG 0.637828 6_4:-26627528 Msa0983530:CDS
GCAATGGCTTTCTCTGATAG+GGG 0.641776 6_4:-26627859 Msa0983530:CDS
GGTCCCGACCTTATCAGCAC+CGG 0.654281 6_4:+26627524 None:intergenic
AAACCTGAGACGGACGACCA+CGG 0.659367 6_4:+26627503 None:intergenic
ACCACTACATAAGCCACTGG+TGG 0.667213 6_4:+26627781 None:intergenic
AGAAAAGAAAGTAGGTTTCG+GGG 0.671452 6_4:-26627571 Msa0983530:intron
GAAAAGAAAGTAGGTTTCGG+GGG 0.676234 6_4:-26627570 Msa0983530:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTTGTCAGAAAAGAAAGT+AGG - chr6_4:26627641-26627660 Msa0983530:intron 25.0%
! ATTCCTTTCTATTGAAAAGA+AGG - chr6_4:26627791-26627810 Msa0983530:CDS 25.0%
TCAGAAAAGAAAGTAGGTTT+CGG - chr6_4:26627647-26627666 Msa0983530:intron 30.0%
! ACTCCTTCTTTTCAATAGAA+AGG + chr6_4:26627797-26627816 None:intergenic 30.0%
ACCTTTGGATTCTTAAGTAG+GGG + chr6_4:26627491-26627510 None:intergenic 35.0%
GACCTTTGGATTCTTAAGTA+GGG + chr6_4:26627492-26627511 None:intergenic 35.0%
TGACCTTTGGATTCTTAAGT+AGG + chr6_4:26627493-26627512 None:intergenic 35.0%
GTTGTTGTGATTGAGTTTGA+GGG + chr6_4:26627595-26627614 None:intergenic 35.0%
TGTTGTTGTGATTGAGTTTG+AGG + chr6_4:26627596-26627615 None:intergenic 35.0%
CAGAAAAGAAAGTAGGTTTC+GGG - chr6_4:26627648-26627667 Msa0983530:intron 35.0%
AGAAAAGAAAGTAGGTTTCG+GGG - chr6_4:26627649-26627668 Msa0983530:intron 35.0%
! TTCATTGATTAGTACACCTC+CGG - chr6_4:26627677-26627696 Msa0983530:intron 35.0%
GTCAATCTCAGAGATCTTAT+CGG + chr6_4:26627748-26627767 None:intergenic 35.0%
CCTTTGGATTCTTAAGTAGG+GGG + chr6_4:26627490-26627509 None:intergenic 40.0%
AAGAATCCAAAGGTCACCTT+TGG - chr6_4:26627497-26627516 Msa0983530:CDS 40.0%
GAAAAGAAAGTAGGTTTCGG+GGG - chr6_4:26627650-26627669 Msa0983530:intron 40.0%
AGCAGCACCTTGTATAGGTT+GGG + chr6_4:26627406-26627425 None:intergenic 45.0%
TTCTTAAGTAGGGGGCAAAG+AGG + chr6_4:26627482-26627501 None:intergenic 45.0%
CCCCCTACTTAAGAATCCAA+AGG - chr6_4:26627487-26627506 Msa0983530:CDS 45.0%
GCTTTGGTTACGAAAGTAGC+CGG - chr6_4:26627533-26627552 Msa0983530:CDS 45.0%
CTTTGGTTACGAAAGTAGCC+GGG - chr6_4:26627534-26627553 Msa0983530:CDS 45.0%
TTTGGTTACGAAAGTAGCCG+GGG - chr6_4:26627535-26627554 Msa0983530:CDS 45.0%
TCACAACAACATGTCGTGAC+CGG - chr6_4:26627605-26627624 Msa0983530:intron 45.0%
CAAAATCCACCAAGCCTAAC+CGG + chr6_4:26627627-26627646 None:intergenic 45.0%
ATCTCAGAGATCTTATCGGC+CGG + chr6_4:26627744-26627763 None:intergenic 45.0%
! AGCACCTTGTATAGGTTGGG+CGG + chr6_4:26627403-26627422 None:intergenic 50.0%
GAGCAGCACCTTGTATAGGT+TGG + chr6_4:26627407-26627426 None:intergenic 50.0%
AAGCGAGCAGCACCTTGTAT+AGG + chr6_4:26627411-26627430 None:intergenic 50.0%
ACCACTACATAAGCCACTGG+TGG + chr6_4:26627442-26627461 None:intergenic 50.0%
CCAGTGGCTTATGTAGTGGT+CGG - chr6_4:26627442-26627461 Msa0983530:CDS 50.0%
CAACAGCCAAAGGTGACCTT+TGG + chr6_4:26627506-26627525 None:intergenic 50.0%
AGCGTCAAGACAACAGCCAA+AGG + chr6_4:26627516-26627535 None:intergenic 50.0%
TGGCTGTTGTCTTGACGCTT+TGG - chr6_4:26627517-26627536 Msa0983530:CDS 50.0%
CCATCCATAAACCTAGACCC+CGG + chr6_4:26627555-26627574 None:intergenic 50.0%
ACAACATGTCGTGACCGGTT+AGG - chr6_4:26627610-26627629 Msa0983530:intron 50.0%
AAGAAAGTAGGTTTCGGGGG+TGG - chr6_4:26627653-26627672 Msa0983530:intron 50.0%
AGAAAGTAGGTTTCGGGGGT+GGG - chr6_4:26627654-26627673 Msa0983530:intron 50.0%
AATAGCCAAACCAACCCAGC+TGG + chr6_4:26627774-26627793 None:intergenic 50.0%
AGCTCCGCCCAACCTATACA+AGG - chr6_4:26627396-26627415 Msa0983530:CDS 55.0%
GCCACCAGTGGCTTATGTAG+TGG - chr6_4:26627438-26627457 Msa0983530:CDS 55.0%
CCGACCACTACATAAGCCAC+TGG + chr6_4:26627445-26627464 None:intergenic 55.0%
TACGAAAGTAGCCGGGGTCT+AGG - chr6_4:26627541-26627560 Msa0983530:CDS 55.0%
GTAGCCGGGGTCTAGGTTTA+TGG - chr6_4:26627548-26627567 Msa0983530:CDS 55.0%
CCGGGGTCTAGGTTTATGGA+TGG - chr6_4:26627552-26627571 Msa0983530:CDS 55.0%
ATGTCGTGACCGGTTAGGCT+TGG - chr6_4:26627615-26627634 Msa0983530:intron 55.0%
!! GTACACCTCCGGTGCTGATA+AGG - chr6_4:26627688-26627707 Msa0983530:intron 55.0%
!! ACCTCCGGTGCTGATAAGGT+CGG - chr6_4:26627692-26627711 Msa0983530:intron 55.0%
AAACCTGAGACGGACGACCA+CGG + chr6_4:26627720-26627739 None:intergenic 55.0%
! TCTGAGATTGACCAGCCAGC+TGG - chr6_4:26627756-26627775 Msa0983530:intron 55.0%
CTGAGATTGACCAGCCAGCT+GGG - chr6_4:26627757-26627776 Msa0983530:intron 55.0%
TGCGCGAGTCACCTTCTTCT+AGG - chr6_4:26627825-26627844 Msa0983530:CDS 55.0%
CGCTTTCTCTCAGCCACCAG+TGG - chr6_4:26627426-26627445 Msa0983530:CDS 60.0%
TCGTGACCGGTTAGGCTTGG+TGG - chr6_4:26627618-26627637 Msa0983530:intron 60.0%
!! CCTCCGGTGCTGATAAGGTC+GGG - chr6_4:26627693-26627712 Msa0983530:intron 60.0%
GGTCCCGACCTTATCAGCAC+CGG + chr6_4:26627699-26627718 None:intergenic 60.0%
TGCTGATAAGGTCGGGACCG+TGG - chr6_4:26627700-26627719 Msa0983530:intron 60.0%
GTCGTCCGTCTCAGGTTTGC+CGG - chr6_4:26627722-26627741 Msa0983530:intron 60.0%
ATCGGCCGGCAAACCTGAGA+CGG + chr6_4:26627730-26627749 None:intergenic 60.0%
GATTGACCAGCCAGCTGGGT+TGG - chr6_4:26627761-26627780 Msa0983530:intron 60.0%
!! ACCAGCCAGCTGGGTTGGTT+TGG - chr6_4:26627766-26627785 Msa0983530:intron 60.0%
CACCTTCTTCTAGGCTCCGC+TGG - chr6_4:26627834-26627853 Msa0983530:CDS 60.0%
GTCCAGCGGAGCCTAGAAGA+AGG + chr6_4:26627839-26627858 None:intergenic 60.0%
GAGAATGCCGTGTCGTCCAG+CGG + chr6_4:26627853-26627872 None:intergenic 60.0%
! GGGGGCAAAGAGGAGCACGT+AGG + chr6_4:26627472-26627491 None:intergenic 65.0%
! CCCGACCTTATCAGCACCGG+AGG + chr6_4:26627696-26627715 None:intergenic 65.0%
GCCAAACCAACCCAGCTGGC+TGG + chr6_4:26627770-26627789 None:intergenic 65.0%
CTAGGCTCCGCTGGACGACA+CGG - chr6_4:26627843-26627862 Msa0983530:CDS 65.0%
GGACCGTGGTCGTCCGTCTC+AGG - chr6_4:26627714-26627733 Msa0983530:intron 70.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 26627364 26627878 26627364 ID=Msa0983530;Name=Msa0983530
chr6_4 mRNA 26627364 26627878 26627364 ID=Msa0983530-mRNA-1;Parent=Msa0983530;Name=Msa0983530-mRNA-1;_AED=0.27;_eAED=0.30;_QI=0|0|0|1|0|0|2|0|103
chr6_4 exon 26627783 26627878 26627783 ID=Msa0983530-mRNA-1:exon:7553;Parent=Msa0983530-mRNA-1
chr6_4 exon 26627364 26627579 26627364 ID=Msa0983530-mRNA-1:exon:7552;Parent=Msa0983530-mRNA-1
chr6_4 CDS 26627783 26627878 26627783 ID=Msa0983530-mRNA-1:cds;Parent=Msa0983530-mRNA-1
chr6_4 CDS 26627364 26627579 26627364 ID=Msa0983530-mRNA-1:cds;Parent=Msa0983530-mRNA-1
Gene Sequence

>Msa0983530

ATGGCTTTCTCTGATAGGGGCTCGCTTCTTCAAGCTCCGCCCAACCTATACAAGGTGCTGCTCGCTTTCTCTCAGCCACCAGTGGCTTATGTAGTGGTTTCGGGGGTGGGTTCATTGATTAGTACACCTCCGGTGCTGATAAGGTCGGGACCGTGGTCGTCCGTCTCAGGTTTGCCGGCCGATAAGATCTCTGAGATTGACCAGCCAGCTGGGTTGGTTTGGCTATTCCTTTCTATTGAAAAGAAGGAGTCAGCTGTCTGCGCGAGTCACCTTCTTCTAGGCTCCGCTGGACGACACGGCATTCTCGTTTAA

Protein sequence

>Msa0983530

MAFSDRGSLLQAPPNLYKVLLAFSQPPVAYVVVSGVGSLISTPPVLIRSGPWSSVSGLPADKISEIDQPAGLVWLFLSIEKKESAVCASHLLLGSAGRHGILV*