AlfalfaGEDB | Msa0986400

M. sativa cultivar zhongmu-4 / Msa0986400

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0986400	AES91278.1	87.719	57	7	0	334	390	28	84	1.97e-22	101

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0986400	G7JS14	87.719	57	7	0	334	390	28	84	9.41e-23	101

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0102070	Msa0986400	0.818766	1.651225e-52	-8.615850e-47
Msa0123810	Msa0986400	0.802786	4.754837e-49	-8.615850e-47
Msa0153910	Msa0986400	0.806021	1.006750e-49	-8.615850e-47
Msa0200000	Msa0986400	0.807511	4.877282e-50	-8.615850e-47
Msa0231000	Msa0986400	0.800085	1.700444e-48	-8.615850e-47
Msa0267000	Msa0986400	0.817547	3.115607e-52	-8.615850e-47
Msa0267010	Msa0986400	0.820035	8.483153e-53	-8.615850e-47
Msa0342240	Msa0986400	0.802690	4.978627e-49	-8.615850e-47
Msa0384800	Msa0986400	0.822764	1.989430e-53	-8.615850e-47
Msa0414420	Msa0986400	0.818446	1.951807e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0986400	MtrunA17_Chr3g0105741	50.685	73	25	2	121	187	22	89	6.92e-11	61.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 111 sgRNAs with CRISPR-Local

Find 901 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAAGAACCACATAAATGG+TGG	0.656013	6_4:-34619134	Msa0986400:CDS
AAGAAGATGAAGAAAGAGGA+GGG	0.489712	6_4:-34617182	Msa0986400:CDS
AAGGAGTTATTACCTAATGG+TGG	0.609589	6_4:+34615329	None:intergenic
AATAGCTCGCTAGGCGAGCT+AGG	0.471681	6_4:-34621122	Msa0986400:CDS
AATCCGTCTTTCCTCCTTGA+CGG	0.500133	6_4:+34615827	None:intergenic
AATCGTCACTACCCCACCAT+TGG	0.489588	6_4:-34615439	Msa0986400:CDS
AATGAAGGAAAAGGGGTTGG+TGG	0.522680	6_4:-34610478	Msa0986400:CDS
ACACCAACAACAACAATAAC+AGG	0.369665	6_4:-34619187	Msa0986400:CDS
ACAGGTTTCTAATGATCCTA+AGG	0.449536	6_4:-34621240	Msa0986400:intron
ACATAGCACTTGGAGATGCT+TGG	0.495639	6_4:-34621214	Msa0986400:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCTTCATGTCAAGTTAGAA+AGG	-	chr6_4:34610436-34610455	Msa0986400:CDS	30.0%
TATGCTAAACAGATGAGATA+AGG	-	chr6_4:34610699-34610718	Msa0986400:intron	30.0%
GTTTATGCGAATAAAGAAAG+AGG	-	chr6_4:34610828-34610847	Msa0986400:intron	30.0%
TAATCTCAGAGATTGGTATA+TGG	-	chr6_4:34610928-34610947	Msa0986400:intron	30.0%
GGACAAAATTTATTTAAGGG+GGG	-	chr6_4:34610965-34610984	Msa0986400:intron	30.0%
GCATCAATGGAGATATTTAT+TGG	-	chr6_4:34611162-34611181	Msa0986400:intron	30.0%
TAACCTCTTGTAGAATCATA+GGG	+	chr6_4:34611354-34611373	None:intergenic	30.0%
ATATTCAAGCTTAGGGTTTA+TGG	-	chr6_4:34611419-34611438	Msa0986400:intron	30.0%
TATTCAAGCTTAGGGTTTAT+GGG	-	chr6_4:34611420-34611439	Msa0986400:intron	30.0%
TGATGCTTCAAGAATTGATT+AGG	-	chr6_4:34611727-34611746	Msa0986400:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_4	gene	34610425	34622737	34610425	ID=Msa0986400;Name=Msa0986400
chr6_4	mRNA	34610425	34622737	34610425	ID=Msa0986400-mRNA-1;Parent=Msa0986400;Name=Msa0986400-mRNA-1;_AED=0.54;_eAED=0.54;_QI=0\|0\|0\|0.3\|1\|1\|10\|0\|390
chr6_4	exon	34622705	34622737	34622705	ID=Msa0986400-mRNA-1:exon:8963;Parent=Msa0986400-mRNA-1
chr6_4	exon	34621666	34621746	34621666	ID=Msa0986400-mRNA-1:exon:8962;Parent=Msa0986400-mRNA-1
chr6_4	exon	34621059	34621258	34621059	ID=Msa0986400-mRNA-1:exon:8961;Parent=Msa0986400-mRNA-1
chr6_4	exon	34620308	34620381	34620308	ID=Msa0986400-mRNA-1:exon:8960;Parent=Msa0986400-mRNA-1
chr6_4	exon	34619112	34619266	34619112	ID=Msa0986400-mRNA-1:exon:8959;Parent=Msa0986400-mRNA-1
chr6_4	exon	34617179	34617232	34617179	ID=Msa0986400-mRNA-1:exon:8958;Parent=Msa0986400-mRNA-1
chr6_4	exon	34616773	34616859	34616773	ID=Msa0986400-mRNA-1:exon:8957;Parent=Msa0986400-mRNA-1
chr6_4	exon	34615779	34615910	34615779	ID=Msa0986400-mRNA-1:exon:8956;Parent=Msa0986400-mRNA-1
chr6_4	exon	34615342	34615504	34615342	ID=Msa0986400-mRNA-1:exon:8955;Parent=Msa0986400-mRNA-1
chr6_4	exon	34610425	34610618	34610425	ID=Msa0986400-mRNA-1:exon:8954;Parent=Msa0986400-mRNA-1
chr6_4	CDS	34622705	34622737	34622705	ID=Msa0986400-mRNA-1:cds;Parent=Msa0986400-mRNA-1
chr6_4	CDS	34621666	34621746	34621666	ID=Msa0986400-mRNA-1:cds;Parent=Msa0986400-mRNA-1
chr6_4	CDS	34621059	34621258	34621059	ID=Msa0986400-mRNA-1:cds;Parent=Msa0986400-mRNA-1
chr6_4	CDS	34620308	34620381	34620308	ID=Msa0986400-mRNA-1:cds;Parent=Msa0986400-mRNA-1
chr6_4	CDS	34619112	34619266	34619112	ID=Msa0986400-mRNA-1:cds;Parent=Msa0986400-mRNA-1
chr6_4	CDS	34617179	34617232	34617179	ID=Msa0986400-mRNA-1:cds;Parent=Msa0986400-mRNA-1
chr6_4	CDS	34616773	34616859	34616773	ID=Msa0986400-mRNA-1:cds;Parent=Msa0986400-mRNA-1
chr6_4	CDS	34615779	34615910	34615779	ID=Msa0986400-mRNA-1:cds;Parent=Msa0986400-mRNA-1
chr6_4	CDS	34615342	34615504	34615342	ID=Msa0986400-mRNA-1:cds;Parent=Msa0986400-mRNA-1
chr6_4	CDS	34610425	34610618	34610425	ID=Msa0986400-mRNA-1:cds;Parent=Msa0986400-mRNA-1

Gene Sequence

>Msa0986400

ATGGATTTCGCTTCTTCATGTCAAGTTAGAAAGAAGATATTCAAGCTTAGGGTTTATGGGTTGGTGAAGTTGAAGCTTGAGCTTTGCTTATGTTATGGTTTTATCTCCATGAAGGTTTCTAATGATCCTAAGGGTCACATAGCACTTGGAGATGCTTGGTTTGAGGTTTTTAAGTCAAAAATATCAATTTTGAGGTTGCTGGACTGTCACAGCTCGCCACGGCGAGCAAATAGCTCGCTAGGCGAGCTAGGAGGTCGAACAGGGGGTCCTGCTCGCCCCCAACTCGCCATGGCGAGTAAGAGCTCGCCTCCCCAGTTGGGTACAAAAGGTTCTCCAAGGGGGAATGGGGTAAGCCGTAAGGGACCCGCACGTTCACTAAGGGAGTCCGCCTTTGCTAAACAAGAGGCAAGCATTAGTAGAGTAAAAAAATTTCCGCAAGACGAGGACACCAACAACAACAATAACAGGAATATAGAAAAATCAAATATTGGTCATGTTAAGAAGAACCACATAAATGGTGGTAGTGCAACTACAAGTGACAATGTTGGTTTGAAGATGAAGAAGAGGGAGGAAGAAGATGAAGAAAGAGGAGGGGTGATCCGGAAGCATGAGTATTCTGATGATTTAGATTTCATCATAGTTTTTGTAGGCATTTTGCCGTTATATGCTATTAACTTGGGTGCTATGTGCACCACCACTTTACTTGCATCTTCTTCTACTGTATCACTTTACCTCCCTGACGTCGCCGTCAAGGAGGAAAGACGGATTGACGATCATCTGTCCAAAAGTAAGGGTCATCTCCAACTAACAAACATGTACCCCATCGTTGGGCATTATTCTGAATCATCTAATGCATATGAATCGTCACTACCCCACCATTGGGTAATGACTCCTAAAACTCGATGTTTAGTACCCCCACCGTTGGGCATTTGTCTGAGTCATCTAATGATGAATGTCTCCGTAAATGCACCACCATTAGGAACTGATACTATTGAGCTTCTACAACTCATAAAATTCAACATTAAAGAAAATATTGTTGACAAAGAAGATGTAGAACTGATGTTAGATGAGGTACTGGGTGTTGAGTTGGTAGAGAGAATGAAGGAAAAGGGGTTGGTGGTTGAGGAATGGGTTGTGTTAGTTTATGATTGCAGCAATATTGATGGAGGTTAG

Protein sequence

>Msa0986400

MDFASSCQVRKKIFKLRVYGLVKLKLELCLCYGFISMKVSNDPKGHIALGDAWFEVFKSKISILRLLDCHSSPRRANSSLGELGGRTGGPARPQLAMASKSSPPQLGTKGSPRGNGVSRKGPARSLRESAFAKQEASISRVKKFPQDEDTNNNNNRNIEKSNIGHVKKNHINGGSATTSDNVGLKMKKREEEDEERGGVIRKHEYSDDLDFIIVFVGILPLYAINLGAMCTTTLLASSSTVSLYLPDVAVKEERRIDDHLSKSKGHLQLTNMYPIVGHYSESSNAYESSLPHHWVMTPKTRCLVPPPLGICLSHLMMNVSVNAPPLGTDTIELLQLIKFNIKENIVDKEDVELMLDEVLGVELVERMKEKGLVVEEWVVLVYDCSNIDGG*