AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0123810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 50 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGGATGTTGTGTTGGAGGC+AGG 0.288739 1_3:-51145882 Msa0123810:CDS
GGAGATCGATTGAAGTTTCT+AGG 0.297705 1_3:+51149167 None:intergenic
TTGGCATAGGAGAGAGGAAC+TGG 0.407774 1_3:+51149090 None:intergenic
CAGGGGCGGATCCAGACTTT+AGG 0.416778 1_3:-51145863 Msa0123810:CDS
TGAGGGGTTTGATGATGAAC+TGG 0.430513 1_3:+51149013 None:intergenic
ATAGGGTCAGGATGTTGTGT+TGG 0.436557 1_3:-51145889 Msa0123810:intron
GGATGTTGTGTTGGAGGCAG+GGG 0.437190 1_3:-51145880 Msa0123810:CDS
AGTTCGATTGAAAGGTTTGT+TGG 0.437877 1_3:+51149071 None:intergenic
GAGCGAGAATGTTTGATTGA+GGG 0.438218 1_3:+51148996 None:intergenic
AGCGAGAATGTTTGATTGAG+GGG 0.442792 1_3:+51148997 None:intergenic
TGAGACGAAGAATGCAAGAT+AGG 0.444236 1_3:-51146879 Msa0123810:CDS
CTCCACATGTACCTAAAGTC+TGG 0.446649 1_3:+51145852 None:intergenic
AGGATGTTGTGTTGGAGGCA+GGG 0.451875 1_3:-51145881 Msa0123810:CDS
TCGACAACCACTTTCTCGCT+TGG 0.456801 1_3:-51146830 Msa0123810:CDS
GAATAACAGAGGCAACATAA+TGG 0.456836 1_3:-51146911 Msa0123810:CDS
TTGAAAGGTTTGTTTGAGGA+AGG 0.460734 1_3:+51149044 None:intergenic
AGAATGCAAGATAGGAAGAT+TGG 0.463858 1_3:-51146871 Msa0123810:CDS
AAATTAATAACAAGCTTTGA+AGG 0.465412 1_3:+51146577 None:intergenic
ATACCTTCACTCTGATTGAC+TGG 0.470507 1_3:+51148882 None:intergenic
AAGGAGATAGTTCGATTGAA+AGG 0.474166 1_3:+51149063 None:intergenic
AAAACTTGAAACTGATTGAA+AGG 0.477211 1_3:+51149119 None:intergenic
ACTGATTGAAAGGGTTGTCC+TGG 0.482471 1_3:+51149129 None:intergenic
AATGAATGGTGATGTGAAGA+TGG 0.507580 1_3:-51146954 Msa0123810:five_prime_UTR
GGGTCAGGATGTTGTGTTGG+AGG 0.511868 1_3:-51145886 Msa0123810:CDS
AGAGCGAGAATGTTTGATTG+AGG 0.513156 1_3:+51148995 None:intergenic
CTGATTGAAAGGGTTGTCCT+GGG 0.513429 1_3:+51149130 None:intergenic
ATCAAGGAGGAACTGTGAAA+AGG 0.525250 1_3:-51146981 Msa0123810:five_prime_UTR
TCGATTGAAAGGTTTGTTTG+AGG 0.539328 1_3:+51149040 None:intergenic
TGAATGACCAAGCGAGAAAG+TGG 0.539344 1_3:+51146823 None:intergenic
TGTTGTGTTGGAGGCAGGGG+CGG 0.539891 1_3:-51145877 Msa0123810:CDS
AAACTTGAAACTGATTGAAA+GGG 0.551865 1_3:+51149120 None:intergenic
ATTGAAAGGTTTGTTGGCAT+AGG 0.554773 1_3:+51149077 None:intergenic
TTGACTGGATACTGTCGAGA+TGG 0.562933 1_3:+51148897 None:intergenic
GAACTGGAGATTCGATTGAA+AGG 0.568624 1_3:+51149029 None:intergenic
TCGATTGAAGTTTCTAGGTG+AGG 0.571334 1_3:+51149172 None:intergenic
GTGATGTGAAGATGGGAGGA+AGG 0.580587 1_3:-51146946 Msa0123810:five_prime_UTR
TGTGAAAAGGAAGCAATGAA+TGG 0.589897 1_3:-51146968 Msa0123810:five_prime_UTR
GAATAAACTGAGACATGTGA+TGG 0.594772 1_3:+51146633 None:intergenic
ATGAATGGTGATGTGAAGAT+GGG 0.596780 1_3:-51146953 Msa0123810:five_prime_UTR
ATCCAGACTTTAGGTACATG+TGG 0.613314 1_3:-51145854 Msa0123810:CDS
GGTTTGTTGGCATAGGAGAG+AGG 0.614080 1_3:+51149084 None:intergenic
TATCCAGTCAATCAGAGTGA+AGG 0.616210 1_3:-51148885 Msa0123810:intron
TGACTGGATACTGTCGAGAT+GGG 0.625151 1_3:+51148898 None:intergenic
GTAAGAAGAATGAATAACAG+AGG 0.630325 1_3:-51146922 Msa0123810:exon
TCCAATCTCACATAAAACCC+AGG 0.646794 1_3:-51149147 Msa0123810:five_prime_UTR
ATTGCAAAACTTGAGAACGA+TGG 0.648935 1_3:+51148929 None:intergenic
CAACTGAAACAAGGTGACAG+AGG 0.660833 1_3:-51146802 Msa0123810:intron
AATGGTGATGTGAAGATGGG+AGG 0.673078 1_3:-51146950 Msa0123810:five_prime_UTR
TTGGTCATTCAACTGAAACA+AGG 0.674150 1_3:-51146811 Msa0123810:CDS
TACAAGACATGCAATCAAGG+AGG 0.698183 1_3:-51146994 Msa0123810:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATAATATATATATATAT+TGG - chr1_3:51148522-51148541 Msa0123810:intron 0.0%
!!! TATTTATTTTTTTACAATCA+TGG - chr1_3:51146466-51146485 Msa0123810:intron 10.0%
!!! ATTTATTTTTTTACAATCAT+GGG - chr1_3:51146467-51146486 Msa0123810:intron 10.0%
!! ATAATATGAATACAAAATAA+TGG - chr1_3:51146565-51146584 Msa0123810:intron 10.0%
!! TAAAAATTAAATACTTATTC+CGG + chr1_3:51147693-51147712 None:intergenic 10.0%
!!! ATTTTAAAAAACTCAATAAT+TGG + chr1_3:51148328-51148347 None:intergenic 10.0%
!!! TTTTGTGCATTTAATTTAAT+AGG - chr1_3:51146321-51146340 Msa0123810:intron 15.0%
!!! TTCTTTATTTCTAAATTATC+TGG - chr1_3:51147449-51147468 Msa0123810:intron 15.0%
!!! TTAATTTCTTTTTCAATTGA+TGG - chr1_3:51149109-51149128 Msa0123810:five_prime_UTR 15.0%
!!! ATTGATTTCTCTGTTTTTTT+AGG - chr1_3:51146277-51146296 Msa0123810:intron 20.0%
!! AAATATTTAAGCATTCACAT+GGG + chr1_3:51146660-51146679 None:intergenic 20.0%
!! TGAATGCTTAAATATTTGTT+TGG - chr1_3:51146663-51146682 Msa0123810:CDS 20.0%
!! ATAACAAACAATTACATTCA+TGG + chr1_3:51146736-51146755 None:intergenic 20.0%
!!! GATTTATTTTAGTCTTCATT+TGG - chr1_3:51146771-51146790 Msa0123810:intron 20.0%
!! TTTATGATCAAATATATACC+TGG - chr1_3:51147104-51147123 Msa0123810:intron 20.0%
!! TTATGATCAAATATATACCT+GGG - chr1_3:51147105-51147124 Msa0123810:intron 20.0%
!! CATATATATTTAAGAATTGC+TGG - chr1_3:51147291-51147310 Msa0123810:intron 20.0%
!!! ATATAGCTTTTATAACAAGT+AGG + chr1_3:51147365-51147384 None:intergenic 20.0%
!! ATAAAAGCTATATAGTAGAT+TGG - chr1_3:51147372-51147391 Msa0123810:intron 20.0%
!!! AGAATATTTTTCTTAGAGTA+TGG + chr1_3:51147433-51147452 None:intergenic 20.0%
!! TGTTTAGTATTGCAATTTAT+TGG - chr1_3:51147548-51147567 Msa0123810:intron 20.0%
!!! TATTGCAATTTATTGGTTTA+TGG - chr1_3:51147555-51147574 Msa0123810:intron 20.0%
!! AGTTGTTAAATATAAGTTGT+TGG - chr1_3:51147586-51147605 Msa0123810:intron 20.0%
!! GTTGTTAAATATAAGTTGTT+GGG - chr1_3:51147587-51147606 Msa0123810:intron 20.0%
!!! TAAAATTTTCTCAACCATAA+GGG + chr1_3:51147729-51147748 None:intergenic 20.0%
!!! TTAAAATTTTCTCAACCATA+AGG + chr1_3:51147730-51147749 None:intergenic 20.0%
!!! TATGGTTGAGAAAATTTTAA+TGG - chr1_3:51147730-51147749 Msa0123810:intron 20.0%
!! CTTGTAAAAGAATACATAAA+TGG + chr1_3:51148018-51148037 None:intergenic 20.0%
!!! AAATTAATAACAAGCTTTGA+AGG + chr1_3:51148452-51148471 None:intergenic 20.0%
!!! GATTTTGAATCTCATATTTA+AGG + chr1_3:51148581-51148600 None:intergenic 20.0%
! AAACTTGAAACTGATTGAAA+GGG + chr1_3:51145909-51145928 None:intergenic 25.0%
! AAAACTTGAAACTGATTGAA+AGG + chr1_3:51145910-51145929 None:intergenic 25.0%
! AATTGAGATCAATGAATGAA+AGG + chr1_3:51146195-51146214 None:intergenic 25.0%
! GAATTGCAATTAAAGTTTGT+TGG - chr1_3:51146232-51146251 Msa0123810:intron 25.0%
! TTTAGGTGATGTTATTCTTT+AGG - chr1_3:51146294-51146313 Msa0123810:intron 25.0%
!! ATCATGGGAGTATATTTATT+TGG - chr1_3:51146482-51146501 Msa0123810:intron 25.0%
!! TCATGGGAGTATATTTATTT+GGG - chr1_3:51146483-51146502 Msa0123810:intron 25.0%
! ATTTATTTGGGTAGAAATCA+TGG - chr1_3:51146495-51146514 Msa0123810:intron 25.0%
! TTTATTTGGGTAGAAATCAT+GGG - chr1_3:51146496-51146515 Msa0123810:intron 25.0%
!!! TACAAAATAATGGAGTGTTT+TGG - chr1_3:51146575-51146594 Msa0123810:intron 25.0%
!!! ACAAAATAATGGAGTGTTTT+GGG - chr1_3:51146576-51146595 Msa0123810:CDS 25.0%
! CAAATATTTAAGCATTCACA+TGG + chr1_3:51146661-51146680 None:intergenic 25.0%
! ATGAAGACTAAAATAAATCG+TGG + chr1_3:51146770-51146789 None:intergenic 25.0%
!!! TTATTTTAGTCTTCATTTGG+TGG - chr1_3:51146774-51146793 Msa0123810:intron 25.0%
! CAACTAAAGGAAAACATATA+AGG + chr1_3:51146837-51146856 None:intergenic 25.0%
!! TATATGTTTTCCTTTAGTTG+CGG - chr1_3:51146837-51146856 Msa0123810:CDS 25.0%
! CATGAGTAAAGTTAAGATAA+AGG + chr1_3:51147031-51147050 None:intergenic 25.0%
! CTTTATCTTAACTTTACTCA+TGG - chr1_3:51147029-51147048 Msa0123810:intron 25.0%
!!! AAGTGAAAGTTTGTTTCTTT+TGG - chr1_3:51147078-51147097 Msa0123810:intron 25.0%
! ACTTATTAAAATTGTTACCC+AGG + chr1_3:51147125-51147144 None:intergenic 25.0%
! GTTTAATTACTCACATCAAA+GGG + chr1_3:51147247-51147266 None:intergenic 25.0%
! AGTTTAATTACTCACATCAA+AGG + chr1_3:51147248-51147267 None:intergenic 25.0%
! TCAAACTAATACATGTCTTT+CGG - chr1_3:51147401-51147420 Msa0123810:intron 25.0%
!!! TAGGCGTTTTATTATCTTTT+GGG - chr1_3:51147491-51147510 Msa0123810:intron 25.0%
! AAACATCTATCTAGATTGAT+AGG + chr1_3:51147533-51147552 None:intergenic 25.0%
! AAGCTAGAAACTATTTGAAT+TGG + chr1_3:51147650-51147669 None:intergenic 25.0%
!!! TTCAAATAGTTTCTAGCTTA+TGG - chr1_3:51147651-51147670 Msa0123810:intron 25.0%
! GAAAACAACAGTAAATTCAT+GGG - chr1_3:51147913-51147932 Msa0123810:intron 25.0%
!!! AGGAAGGAAAAAGTATTTTA+CGG + chr1_3:51147968-51147987 None:intergenic 25.0%
!!! TACTTTTTCCTTCCTATTTA+TGG - chr1_3:51147973-51147992 Msa0123810:intron 25.0%
!!! TTGAGTTTTTTAAAATCTGC+AGG - chr1_3:51148332-51148351 Msa0123810:intron 25.0%
!!! ATATATATATATTGGCCTTC+AGG - chr1_3:51148530-51148549 Msa0123810:intron 25.0%
!! TCGTTGAAGAATTTTATGAA+AGG - chr1_3:51148821-51148840 Msa0123810:intron 25.0%
!! TTTGTGTTGCAACATTATTT+TGG - chr1_3:51148925-51148944 Msa0123810:five_prime_UTR 25.0%
!! TTGTGTTGCAACATTATTTT+GGG - chr1_3:51148926-51148945 Msa0123810:five_prime_UTR 25.0%
!!! ACATTATTTTGGGTAAATTG+TGG - chr1_3:51148936-51148955 Msa0123810:five_prime_UTR 25.0%
!!! TTTCAATTGATGGCTTTTAT+AGG - chr1_3:51149119-51149138 Msa0123810:five_prime_UTR 25.0%
!!! TTCAATTGATGGCTTTTATA+GGG - chr1_3:51149120-51149139 Msa0123810:five_prime_UTR 25.0%
CTTTAATTGCAATTCAACTG+AGG + chr1_3:51146227-51146246 None:intergenic 30.0%
AAACAGAGAAATCAATCCAT+TGG + chr1_3:51146273-51146292 None:intergenic 30.0%
TTATTTGGGTAGAAATCATG+GGG - chr1_3:51146497-51146516 Msa0123810:intron 30.0%
AATACAATAACCGCAACTAA+AGG + chr1_3:51146850-51146869 None:intergenic 30.0%
TCTAAATAAATACAACCGCT+TGG + chr1_3:51146969-51146988 None:intergenic 30.0%
TATCTTAACTTTACTCATGG+TGG - chr1_3:51147032-51147051 Msa0123810:intron 30.0%
AAGGGTTTGAATAGTCATAA+GGG + chr1_3:51147229-51147248 None:intergenic 30.0%
AAAGGGTTTGAATAGTCATA+AGG + chr1_3:51147230-51147249 None:intergenic 30.0%
!! GTAGGCGTTTTATTATCTTT+TGG - chr1_3:51147490-51147509 Msa0123810:intron 30.0%
!! ATTATCTTTTGGGTGAAAGT+TGG - chr1_3:51147501-51147520 Msa0123810:intron 30.0%
AACTTAAGAAAAAGCCCTTA+TGG - chr1_3:51147712-51147731 Msa0123810:intron 30.0%
GAAAACTTTCTCATAAAGAG+AGG - chr1_3:51147846-51147865 Msa0123810:intron 30.0%
CGAAAACAACAGTAAATTCA+TGG - chr1_3:51147912-51147931 Msa0123810:intron 30.0%
AATTCCACAGAACCATAAAT+AGG + chr1_3:51147988-51148007 None:intergenic 30.0%
! TTTTACAAGACATGCAATCA+AGG - chr1_3:51148029-51148048 Msa0123810:intron 30.0%
GTAAGAAGAATGAATAACAG+AGG - chr1_3:51148104-51148123 Msa0123810:intron 30.0%
!! TTTGTTGTCAGTCTCATTTT+GGG + chr1_3:51148504-51148523 None:intergenic 30.0%
!!! ATTTGTTGTCAGTCTCATTT+TGG + chr1_3:51148505-51148524 None:intergenic 30.0%
GAGTGATTAGAAATTGTGAA+AGG - chr1_3:51148612-51148631 Msa0123810:intron 30.0%
TTATCACTTTACGTCTTTCT+TGG - chr1_3:51148746-51148765 Msa0123810:intron 30.0%
! CATCTCTAGATTCTTATTTC+CGG - chr1_3:51148792-51148811 Msa0123810:intron 30.0%
TGTATGTTAGAATAGAGTGA+AGG - chr1_3:51149016-51149035 Msa0123810:five_prime_UTR 30.0%
!! ATTGAAAGGTTTGTTGGCAT+AGG + chr1_3:51145952-51145971 None:intergenic 35.0%
AGTTCGATTGAAAGGTTTGT+TGG + chr1_3:51145958-51145977 None:intergenic 35.0%
AAGGAGATAGTTCGATTGAA+AGG + chr1_3:51145966-51145985 None:intergenic 35.0%
! TTGAAAGGTTTGTTTGAGGA+AGG + chr1_3:51145985-51146004 None:intergenic 35.0%
! TCGATTGAAAGGTTTGTTTG+AGG + chr1_3:51145989-51146008 None:intergenic 35.0%
ATTGCAAAACTTGAGAACGA+TGG + chr1_3:51146100-51146119 None:intergenic 35.0%
! CCACAATACAAGTTAGTTTG+AGG + chr1_3:51146691-51146710 None:intergenic 35.0%
! CCTCAAACTAACTTGTATTG+TGG - chr1_3:51146688-51146707 Msa0123810:intron 35.0%
TTGTGAAGCTGTGTTAAGAA+TGG - chr1_3:51146876-51146895 Msa0123810:CDS 35.0%
!! AGAATTGCTGGTTTATGACT+AGG - chr1_3:51147303-51147322 Msa0123810:intron 35.0%
! ATCTGGAACCAAGATACTTA+TGG - chr1_3:51147466-51147485 Msa0123810:intron 35.0%
AACCAAGATACTTATGGTGT+AGG - chr1_3:51147472-51147491 Msa0123810:intron 35.0%
!!! TTACTGTTGTTTTCGTTTGC+AGG + chr1_3:51147907-51147926 None:intergenic 35.0%
TGTGAAAAGGAAGCAATGAA+TGG - chr1_3:51148058-51148077 Msa0123810:intron 35.0%
! AATGAATGGTGATGTGAAGA+TGG - chr1_3:51148072-51148091 Msa0123810:intron 35.0%
! ATGAATGGTGATGTGAAGAT+GGG - chr1_3:51148073-51148092 Msa0123810:intron 35.0%
GAATAACAGAGGCAACATAA+TGG - chr1_3:51148115-51148134 Msa0123810:intron 35.0%
AGAATGCAAGATAGGAAGAT+TGG - chr1_3:51148155-51148174 Msa0123810:intron 35.0%
TTGGTCATTCAACTGAAACA+AGG - chr1_3:51148215-51148234 Msa0123810:intron 35.0%
GAATAAACTGAGACATGTGA+TGG + chr1_3:51148396-51148415 None:intergenic 35.0%
TTAAGGTCAAGTTACATGCA+AGG + chr1_3:51148564-51148583 None:intergenic 35.0%
ATGAAGAAAGAGAGAGACAA+AGG + chr1_3:51148898-51148917 None:intergenic 35.0%
CACTCTATTCTAACATACAG+AGG + chr1_3:51149015-51149034 None:intergenic 35.0%
GATGAATGAAGACAGTACTA+AGG + chr1_3:51149053-51149072 None:intergenic 35.0%
! TCGATTGAAGTTTCTAGGTG+AGG + chr1_3:51145857-51145876 None:intergenic 40.0%
GGAGATCGATTGAAGTTTCT+AGG + chr1_3:51145862-51145881 None:intergenic 40.0%
TCCAATCTCACATAAAACCC+AGG - chr1_3:51145879-51145898 Msa0123810:CDS 40.0%
!! TCCTGGGTTTTATGTGAGAT+TGG + chr1_3:51145883-51145902 None:intergenic 40.0%
GAACTGGAGATTCGATTGAA+AGG + chr1_3:51146000-51146019 None:intergenic 40.0%
AGCGAGAATGTTTGATTGAG+GGG + chr1_3:51146032-51146051 None:intergenic 40.0%
GAGCGAGAATGTTTGATTGA+GGG + chr1_3:51146033-51146052 None:intergenic 40.0%
AGAGCGAGAATGTTTGATTG+AGG + chr1_3:51146034-51146053 None:intergenic 40.0%
TATCCAGTCAATCAGAGTGA+AGG - chr1_3:51146141-51146160 Msa0123810:intron 40.0%
ATACCTTCACTCTGATTGAC+TGG + chr1_3:51146147-51146166 None:intergenic 40.0%
GCATGAAAAACATCATGACG+AGG + chr1_3:51146373-51146392 None:intergenic 40.0%
! CGTCATGATGTTTTTCATGC+CGG - chr1_3:51146373-51146392 Msa0123810:intron 40.0%
ACGTCATTCACATGGTGTAT+CGG + chr1_3:51146403-51146422 None:intergenic 40.0%
ATCTCACAACGTCATTCACA+TGG + chr1_3:51146411-51146430 None:intergenic 40.0%
TGTGAATGACGTTGTGAGAT+AGG - chr1_3:51146411-51146430 Msa0123810:intron 40.0%
! GGGTGATGATGTATCAAGAA+TGG + chr1_3:51147209-51147228 None:intergenic 40.0%
TTCTCATAAAGAGAGGACGT+AGG - chr1_3:51147853-51147872 Msa0123810:intron 40.0%
CCACAGAACCATAAATAGGA+AGG + chr1_3:51147984-51148003 None:intergenic 40.0%
! CCTTCCTATTTATGGTTCTG+TGG - chr1_3:51147981-51148000 Msa0123810:intron 40.0%
TACAAGACATGCAATCAAGG+AGG - chr1_3:51148032-51148051 Msa0123810:intron 40.0%
ATCAAGGAGGAACTGTGAAA+AGG - chr1_3:51148045-51148064 Msa0123810:intron 40.0%
TGAGACGAAGAATGCAAGAT+AGG - chr1_3:51148147-51148166 Msa0123810:intron 40.0%
TGCAAGGTAACATTTCCTGA+AGG + chr1_3:51148548-51148567 None:intergenic 40.0%
!!! CTTCTGCAGTTTTAGCTCAA+GGG + chr1_3:51148668-51148687 None:intergenic 40.0%
ATAAAAGTAGAACCCACGTG+TGG + chr1_3:51148730-51148749 None:intergenic 40.0%
AATTCTTCAACGAGCTCAAC+CGG + chr1_3:51148814-51148833 None:intergenic 40.0%
!! TTGATGGCTTTTATAGGGTC+AGG - chr1_3:51149125-51149144 Msa0123810:five_prime_UTR 40.0%
ATCCAGACTTTAGGTACATG+TGG - chr1_3:51149172-51149191 Msa0123810:five_prime_UTR 40.0%
CTGATTGAAAGGGTTGTCCT+GGG + chr1_3:51145899-51145918 None:intergenic 45.0%
ACTGATTGAAAGGGTTGTCC+TGG + chr1_3:51145900-51145919 None:intergenic 45.0%
!! TGAGGGGTTTGATGATGAAC+TGG + chr1_3:51146016-51146035 None:intergenic 45.0%
TGACTGGATACTGTCGAGAT+GGG + chr1_3:51146131-51146150 None:intergenic 45.0%
TTGACTGGATACTGTCGAGA+TGG + chr1_3:51146132-51146151 None:intergenic 45.0%
ATCAATCCATTGGCGAAGAG+TGG + chr1_3:51146263-51146282 None:intergenic 45.0%
! TTGGGTGACACATAGTTTGC+AGG - chr1_3:51146594-51146613 Msa0123810:CDS 45.0%
! TGGGTGACACATAGTTTGCA+GGG - chr1_3:51146595-51146614 Msa0123810:CDS 45.0%
AGAGTGAGTGATCTTCCAAG+CGG - chr1_3:51146951-51146970 Msa0123810:five_prime_UTR 45.0%
CGCCTACACCATAAGTATCT+TGG + chr1_3:51147477-51147496 None:intergenic 45.0%
CTTATTCCGGTTGAGTTCAG+AGG + chr1_3:51147680-51147699 None:intergenic 45.0%
GACGTAGGACCATAGAAAAG+AGG - chr1_3:51147868-51147887 Msa0123810:intron 45.0%
! TGCTTGTGGCCTCTTTTCTA+TGG + chr1_3:51147880-51147899 None:intergenic 45.0%
! AATGGTGATGTGAAGATGGG+AGG - chr1_3:51148076-51148095 Msa0123810:intron 45.0%
TGAATGACCAAGCGAGAAAG+TGG + chr1_3:51148206-51148225 None:intergenic 45.0%
CAACTGAAACAAGGTGACAG+AGG - chr1_3:51148224-51148243 Msa0123810:intron 45.0%
!!! GCTTCTGCAGTTTTAGCTCA+AGG + chr1_3:51148669-51148688 None:intergenic 45.0%
TAGTCCATATGTACCACACG+TGG - chr1_3:51148714-51148733 Msa0123810:intron 45.0%
AGTCCATATGTACCACACGT+GGG - chr1_3:51148715-51148734 Msa0123810:intron 45.0%
GAAGACAGTACTAAGGCATG+TGG + chr1_3:51149046-51149065 None:intergenic 45.0%
ATAGGGTCAGGATGTTGTGT+TGG - chr1_3:51149137-51149156 Msa0123810:five_prime_UTR 45.0%
CTCCACATGTACCTAAAGTC+TGG + chr1_3:51149177-51149196 None:intergenic 45.0%
TTGGCATAGGAGAGAGGAAC+TGG + chr1_3:51145939-51145958 None:intergenic 50.0%
!! GGTTTGTTGGCATAGGAGAG+AGG + chr1_3:51145945-51145964 None:intergenic 50.0%
GATTCTCCACTCTTCGCCAA+TGG - chr1_3:51146254-51146273 Msa0123810:intron 50.0%
!! CACATGGTGTATCGGCTTTC+CGG + chr1_3:51146395-51146414 None:intergenic 50.0%
TGGAAGCCTCTGAACTCAAC+CGG - chr1_3:51147671-51147690 Msa0123810:intron 50.0%
GTGATGTGAAGATGGGAGGA+AGG - chr1_3:51148080-51148099 Msa0123810:intron 50.0%
TCGACAACCACTTTCTCGCT+TGG - chr1_3:51148196-51148215 Msa0123810:intron 50.0%
GAACCCACGTGTGGTACATA+TGG + chr1_3:51148721-51148740 None:intergenic 50.0%
AGGATGTTGTGTTGGAGGCA+GGG - chr1_3:51149145-51149164 Msa0123810:five_prime_UTR 50.0%
CGTTTGCAGGAGTCTGCTTG+TGG + chr1_3:51147894-51147913 None:intergenic 55.0%
GGGTCAGGATGTTGTGTTGG+AGG - chr1_3:51149140-51149159 Msa0123810:five_prime_UTR 55.0%
CAGGATGTTGTGTTGGAGGC+AGG - chr1_3:51149144-51149163 Msa0123810:five_prime_UTR 55.0%
GGATGTTGTGTTGGAGGCAG+GGG - chr1_3:51149146-51149165 Msa0123810:five_prime_UTR 55.0%
TGTTGTGTTGGAGGCAGGGG+CGG - chr1_3:51149149-51149168 Msa0123810:five_prime_UTR 60.0%
CAGGGGCGGATCCAGACTTT+AGG - chr1_3:51149163-51149182 Msa0123810:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 51145848 51149200 51145848 ID=Msa0123810;Name=Msa0123810
chr1_3 mRNA 51145848 51149200 51145848 ID=Msa0123810-mRNA-1;Parent=Msa0123810;Name=Msa0123810-mRNA-1;_AED=0.35;_eAED=0.35;_QI=390|0.33|0.5|0.75|0.66|0.5|4|0|103
chr1_3 exon 51145848 51145907 51145848 ID=Msa0123810-mRNA-1:exon:15644;Parent=Msa0123810-mRNA-1
chr1_3 exon 51146576 51146694 51146576 ID=Msa0123810-mRNA-1:exon:15643;Parent=Msa0123810-mRNA-1
chr1_3 exon 51146803 51147010 51146803 ID=Msa0123810-mRNA-1:exon:15642;Parent=Msa0123810-mRNA-1
chr1_3 exon 51148886 51149200 51148886 ID=Msa0123810-mRNA-1:exon:15641;Parent=Msa0123810-mRNA-1
chr1_3 five_prime_UTR 51148886 51149200 51148886 ID=Msa0123810-mRNA-1:five_prime_utr;Parent=Msa0123810-mRNA-1
chr1_3 five_prime_UTR 51146936 51147010 51146936 ID=Msa0123810-mRNA-1:five_prime_utr;Parent=Msa0123810-mRNA-1
chr1_3 CDS 51146803 51146935 51146803 ID=Msa0123810-mRNA-1:cds;Parent=Msa0123810-mRNA-1
chr1_3 CDS 51146576 51146694 51146576 ID=Msa0123810-mRNA-1:cds;Parent=Msa0123810-mRNA-1
chr1_3 CDS 51145848 51145907 51145848 ID=Msa0123810-mRNA-1:cds;Parent=Msa0123810-mRNA-1
Gene Sequence

>Msa0123810

ATGAATAACAGAGGCAACATAATGGACACAAATCTGAGACGAAGAATGCAAGATAGGAAGATTGGACGAGTTGTTTGCGAATGTCGACAACCACTTTCTCGCTTGGTCATTCAACTGAAACAAGGTGACAGAGGTATATGTTTGTTTCAATCTAATTTTCGTTGTCTTTTTTCCATCACATGTCTCAGTTTATTCTCTATTTCTCTTGCTGATTCTCTTAGTTTGTTTCCTTCAAAGCTTGTTATTAATTTTGGTCAGGATGTTGTGTTGGAGGCAGGGGCGGATCCAGACTTTAGGTACATGTGGAGCTAA
Protein sequence

>Msa0123810

MNNRGNIMDTNLRRRMQDRKIGRVVCECRQPLSRLVIQLKQGDRGICLFQSNFRCLFSITCLSLFSISLADSLSLFPSKLVINFGQDVVLEAGADPDFRYMWS*