AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1003310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1003310 MtrunA17_Chr4g0001531 100.000 57 0 0 1 57 1 57 7.78e-39 122
Msa1003310 MtrunA17_Chr7g0214441 100.000 57 0 0 1 57 1 57 7.78e-39 122
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1003310 AT3G05870.1 100.000 56 0 0 1 56 31 86 5.19e-38 120
Msa1003310 AT3G05870.2 100.000 56 0 0 1 56 31 86 5.19e-38 120
Msa1003310 AT3G05870.6 100.000 56 0 0 1 56 31 86 5.19e-38 120
Msa1003310 AT3G05870.4 100.000 56 0 0 1 56 34 89 6.68e-38 120
Msa1003310 AT3G05870.3 100.000 56 0 0 1 56 34 89 6.68e-38 120
Msa1003310 AT3G05870.5 100.000 56 0 0 1 56 34 89 6.68e-38 120
Msa1003310 AT3G05870.7 100.000 56 0 0 1 56 22 77 9.44e-38 120

Find 40 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAATTTGATCATTTCTAT+TGG 0.277487 7_1:+463190 Msa1003310:three_prime_UTR
TGAAATTAGAGTTTCTAAAT+TGG 0.278981 7_1:+463270 Msa1003310:three_prime_UTR
CGTCGGGAATGGCAATTTAA+AGG 0.290239 7_1:+463074 Msa1003310:CDS
ATTTACATTGTATCTTGAAA+TGG 0.311304 7_1:+463012 Msa1003310:CDS
CTGATAATGAAGTTAGGGTT+TGG 0.352667 7_1:-460927 None:intergenic
GATGGCATGTTGTTGCTTCA+TGG 0.372099 7_1:+461249 Msa1003310:intron
ATGTTGTTGCTTCATGGACT+TGG 0.375530 7_1:+461255 Msa1003310:five_prime_UTR
TGTCCTGATTGTAAACTTCC+TGG 0.394301 7_1:+461329 Msa1003310:CDS
ATACATACGTTTGAATTTGC+GGG 0.399310 7_1:+460825 Msa1003310:five_prime_UTR
AAAGAAGATTTGAATTGAAA+AGG 0.400354 7_1:-460901 None:intergenic
CTATTGGATTGGATTTCTGC+TGG 0.407759 7_1:+461048 Msa1003310:five_prime_UTR
TTTACATTGTATCTTGAAAT+GGG 0.412787 7_1:+463013 Msa1003310:CDS
TAATATCTCCATTGTTTCCA+TGG 0.415568 7_1:+463244 Msa1003310:three_prime_UTR
TATACATACGTTTGAATTTG+CGG 0.415740 7_1:+460824 Msa1003310:five_prime_UTR
ATTTGTAGGATGGCCTTTGA+TGG 0.417636 7_1:+461302 Msa1003310:exon
GCCATTCCCGACGACACATT+GGG 0.449214 7_1:-463064 None:intergenic
GTCCTGATTGTAAACTTCCT+GGG 0.462403 7_1:+461330 Msa1003310:CDS
AAACTCTAATTTCAATCCCA+TGG 0.474150 7_1:-463261 None:intergenic
ACTAGACAAGACAAAGGAAA+AGG 0.484810 7_1:+460848 Msa1003310:five_prime_UTR
ATGAAACATGTGGAATTTGT+AGG 0.488299 7_1:+461288 Msa1003310:five_prime_UTR
TTTGAAGCAAAAGCACAAAC+AGG 0.490100 7_1:-460997 None:intergenic
TGCCATTCCCGACGACACAT+TGG 0.494237 7_1:-463065 None:intergenic
GCCCAATGTGTCGTCGGGAA+TGG 0.498823 7_1:+463063 Msa1003310:CDS
TGTTGTTGCTTCATGGACTT+GGG 0.505473 7_1:+461256 Msa1003310:five_prime_UTR
AATATCTCCATTGTTTCCAT+GGG 0.512610 7_1:+463245 Msa1003310:three_prime_UTR
CACATTGCCCAATGTGTCGT+CGG 0.513658 7_1:+463057 Msa1003310:CDS
TCCCCAGGAAGTTTACAATC+AGG 0.518100 7_1:-461332 None:intergenic
CATTGGGCAATGTGCTTGTG+AGG 0.523053 7_1:-463048 None:intergenic
TTTCAATCCCATGGAAACAA+TGG 0.523838 7_1:-463252 None:intergenic
GGATGTATTGCTTATGAATG+AGG 0.540581 7_1:+463211 Msa1003310:three_prime_UTR
ACATTGCCCAATGTGTCGTC+GGG 0.540781 7_1:+463058 Msa1003310:CDS
TTCATGGACTTGGGATGCAC+AGG 0.541212 7_1:+461265 Msa1003310:five_prime_UTR
GATTTGAATTGAAAAGGAGA+AGG 0.548938 7_1:-460895 None:intergenic
TCCTGATTGTAAACTTCCTG+GGG 0.574605 7_1:+461331 Msa1003310:CDS
CTGAGTGGACAGTCATCCCC+AGG 0.578584 7_1:-461347 None:intergenic
ATCAGGACAGCATCCATCAA+AGG 0.590910 7_1:-461315 None:intergenic
CTAGACAAGACAAAGGAAAA+GGG 0.599308 7_1:+460849 Msa1003310:five_prime_UTR
TGCGGGACTAGACAAGACAA+AGG 0.697609 7_1:+460842 Msa1003310:five_prime_UTR
AACATGTGGAATTTGTAGGA+TGG 0.713439 7_1:+461292 Msa1003310:exon
GATGCACAGGATGAAACATG+TGG 0.727134 7_1:+461278 Msa1003310:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAACTAATGAATAAATATT+AGG - chr7_1:461185-461204 None:intergenic 15.0%
!! TCATTCAATAAAATAAACAA+TGG - chr7_1:461829-461848 None:intergenic 15.0%
!! TTATCTAAATGTTAAAACAA+AGG - chr7_1:461855-461874 None:intergenic 15.0%
!! AATATATTATTTGATATGTC+AGG - chr7_1:462916-462935 None:intergenic 15.0%
!! AAAGAAGATTTGAATTGAAA+AGG - chr7_1:460904-460923 None:intergenic 20.0%
!!! ATTTTCTGATAATGAAGTTA+GGG - chr7_1:460935-460954 None:intergenic 20.0%
!!! AATAAATTAAAAGCACAAAC+AGG - chr7_1:461487-461506 None:intergenic 20.0%
!! TTGTTTGTATAAAGTGAAAA+GGG - chr7_1:461658-461677 None:intergenic 20.0%
!! ATTGTTTGTATAAAGTGAAA+AGG - chr7_1:461659-461678 None:intergenic 20.0%
!! TAAAATAGTAAACTCTCATT+TGG + chr7_1:461713-461732 Msa1003310:intron 20.0%
!! AGAAGAATCAACATAAATTT+CGG - chr7_1:462219-462238 None:intergenic 20.0%
!! TACATTCAGTAAAATTAGAA+AGG - chr7_1:462568-462587 None:intergenic 20.0%
!! ATTTACATTGTATCTTGAAA+TGG + chr7_1:463012-463031 Msa1003310:CDS 20.0%
!! TTTACATTGTATCTTGAAAT+GGG + chr7_1:463013-463032 Msa1003310:CDS 20.0%
!!! CAATGTAATTTTGTTTTTCA+TGG + chr7_1:463113-463132 Msa1003310:three_prime_UTR 20.0%
!!! AAGTAACTAAGATCTTTTTA+AGG + chr7_1:463163-463182 Msa1003310:three_prime_UTR 20.0%
!!! AGTAACTAAGATCTTTTTAA+GGG + chr7_1:463164-463183 Msa1003310:three_prime_UTR 20.0%
!! TGTAATTTGATCATTTCTAT+TGG + chr7_1:463190-463209 Msa1003310:three_prime_UTR 20.0%
!! TGAAATTAGAGTTTCTAAAT+TGG + chr7_1:463270-463289 Msa1003310:three_prime_UTR 20.0%
!!! ATATATTTTTTCATCCTTTG+TGG - chr7_1:463312-463331 None:intergenic 20.0%
! TATACATACGTTTGAATTTG+CGG + chr7_1:460824-460843 Msa1003310:five_prime_UTR 25.0%
!!! CATTTTCTGATAATGAAGTT+AGG - chr7_1:460936-460955 None:intergenic 25.0%
! ATCTCTAACTAACTTTATAC+AGG + chr7_1:461087-461106 Msa1003310:intron 25.0%
!!! AATATTAGGTTGATTTCACT+AGG - chr7_1:461171-461190 None:intergenic 25.0%
! TCAGTAAGTTACATTATCTT+GGG + chr7_1:461366-461385 Msa1003310:intron 25.0%
! GTCATTGAAATATGTTGAAA+TGG + chr7_1:461566-461585 Msa1003310:intron 25.0%
! TGAAAAGGGTTAATTCTTAT+TGG - chr7_1:461644-461663 None:intergenic 25.0%
! TCATGGTAACATATATATCA+CGG - chr7_1:461914-461933 None:intergenic 25.0%
! ACTAATTTCATCAATATTCC+AGG + chr7_1:462067-462086 Msa1003310:intron 25.0%
! AACATTCTTCTTAACAGAAT+AGG + chr7_1:462170-462189 Msa1003310:intron 25.0%
! AATGGATGAGATACTTTAAA+GGG - chr7_1:462269-462288 None:intergenic 25.0%
! AAATGGATGAGATACTTTAA+AGG - chr7_1:462270-462289 None:intergenic 25.0%
!!! TAAAGTATCTCATCCATTTT+TGG + chr7_1:462271-462290 Msa1003310:intron 25.0%
! TTTACCACGTCATTAATATA+TGG + chr7_1:462351-462370 Msa1003310:intron 25.0%
! TTACCACGTCATTAATATAT+GGG + chr7_1:462352-462371 Msa1003310:intron 25.0%
! ATACCTATAAACAACATTTG+TGG + chr7_1:462599-462618 Msa1003310:intron 25.0%
!! AAACCACAAATGTTGTTTAT+AGG - chr7_1:462605-462624 None:intergenic 25.0%
!! TTTATAAAGCTTTATGCTCA+AGG + chr7_1:462696-462715 Msa1003310:intron 25.0%
!!! CATGATTGAGAATTTTTCAA+GGG + chr7_1:462737-462756 Msa1003310:intron 25.0%
!!! TTTGTTTTTCATGGAAAACT+AGG + chr7_1:463122-463141 Msa1003310:three_prime_UTR 25.0%
!!! TTGTTTTTCATGGAAAACTA+GGG + chr7_1:463123-463142 Msa1003310:three_prime_UTR 25.0%
ATACATACGTTTGAATTTGC+GGG + chr7_1:460825-460844 Msa1003310:five_prime_UTR 30.0%
GATTTGAATTGAAAAGGAGA+AGG - chr7_1:460898-460917 None:intergenic 30.0%
GGTTGAATGCAAAAATGAAA+AGG - chr7_1:460979-460998 None:intergenic 30.0%
!! TGACTTAGTTTTCTCTCTAT+TGG + chr7_1:461032-461051 Msa1003310:five_prime_UTR 30.0%
! TAGTTTTCTCTCTATTGGAT+TGG + chr7_1:461037-461056 Msa1003310:five_prime_UTR 30.0%
CTAACTTTATACAGGTTGTT+TGG + chr7_1:461095-461114 Msa1003310:intron 30.0%
CTTATCTCATCAAATGTAGA+TGG + chr7_1:461231-461250 Msa1003310:intron 30.0%
ATGAAACATGTGGAATTTGT+AGG + chr7_1:461288-461307 Msa1003310:five_prime_UTR 30.0%
AGATAATGTAACTTACTGAG+TGG - chr7_1:461365-461384 None:intergenic 30.0%
CTCAGTAAGTTACATTATCT+TGG + chr7_1:461365-461384 Msa1003310:intron 30.0%
ATTATCTTGGGATTTGTCTT+TGG + chr7_1:461378-461397 Msa1003310:intron 30.0%
AATTCAGCACAATAGAAAAG+TGG - chr7_1:461539-461558 None:intergenic 30.0%
!! CTTATAAGAGTGTTATGAAG+TGG + chr7_1:461690-461709 Msa1003310:intron 30.0%
TAGTAAACTCTCATTTGGAT+TGG + chr7_1:461718-461737 Msa1003310:intron 30.0%
ATGGAATTTAACAACACTTC+AGG - chr7_1:461810-461829 None:intergenic 30.0%
AACATATATATCACGGTAGT+TGG - chr7_1:461907-461926 None:intergenic 30.0%
ACAATTGCATGATAGTTTCA+TGG - chr7_1:461931-461950 None:intergenic 30.0%
AAATAGATGCTGTAAAAAGC+AGG + chr7_1:461982-462001 Msa1003310:intron 30.0%
AATAGATGCTGTAAAAAGCA+GGG + chr7_1:461983-462002 Msa1003310:intron 30.0%
! GTACTTATTGGTTATGTTGT+AGG - chr7_1:462127-462146 None:intergenic 30.0%
GAACAAAATTAGGCCAAAAA+TGG - chr7_1:462287-462306 None:intergenic 30.0%
AAACCCATATATTAATGACG+TGG - chr7_1:462358-462377 None:intergenic 30.0%
!!! GATTTTTAGTTGAAACCAGT+TGG - chr7_1:462421-462440 None:intergenic 30.0%
! AATGTTGTTTATAGGTATCC+AGG - chr7_1:462597-462616 None:intergenic 30.0%
! GCATGATTGAGAATTTTTCA+AGG + chr7_1:462736-462755 Msa1003310:intron 30.0%
TAATATCTCCATTGTTTCCA+TGG + chr7_1:463244-463263 Msa1003310:three_prime_UTR 30.0%
AATATCTCCATTGTTTCCAT+GGG + chr7_1:463245-463264 Msa1003310:three_prime_UTR 30.0%
AAACTCTAATTTCAATCCCA+TGG - chr7_1:463264-463283 None:intergenic 30.0%
ACTAGACAAGACAAAGGAAA+AGG + chr7_1:460848-460867 Msa1003310:five_prime_UTR 35.0%
CTAGACAAGACAAAGGAAAA+GGG + chr7_1:460849-460868 Msa1003310:five_prime_UTR 35.0%
! CTGATAATGAAGTTAGGGTT+TGG - chr7_1:460930-460949 None:intergenic 35.0%
! TTTGAAGCAAAAGCACAAAC+AGG - chr7_1:461000-461019 None:intergenic 35.0%
AACATGTGGAATTTGTAGGA+TGG + chr7_1:461292-461311 Msa1003310:exon 35.0%
CAATAGAAAAGTGGACAAAC+AGG - chr7_1:461530-461549 None:intergenic 35.0%
ATAGATGCTGTAAAAAGCAG+GGG + chr7_1:461984-462003 Msa1003310:intron 35.0%
CCACAAGATTGTGTACTTAT+TGG - chr7_1:462139-462158 None:intergenic 35.0%
CCAATAAGTACACAATCTTG+TGG + chr7_1:462136-462155 Msa1003310:intron 35.0%
!! CTTCTCTAAGATGACTATGT+TGG + chr7_1:462234-462253 Msa1003310:intron 35.0%
GAGTCAAGAGGAACAAAATT+AGG - chr7_1:462297-462316 None:intergenic 35.0%
TTTCTCACATAAGAGTCAAG+AGG - chr7_1:462309-462328 None:intergenic 35.0%
! CTGATTGCATGATAGTGTAA+TGG - chr7_1:462398-462417 None:intergenic 35.0%
AGCTCAAAAGGCAATAAAAG+GGG - chr7_1:462514-462533 None:intergenic 35.0%
GAGCTCAAAAGGCAATAAAA+GGG - chr7_1:462515-462534 None:intergenic 35.0%
AGAGCTCAAAAGGCAATAAA+AGG - chr7_1:462516-462535 None:intergenic 35.0%
TTACTGAATGTAGATAAGCC+TGG + chr7_1:462576-462595 Msa1003310:intron 35.0%
TGTGTTATCACTGACATTCA+TGG + chr7_1:462667-462686 Msa1003310:intron 35.0%
GTGTTATCACTGACATTCAT+GGG + chr7_1:462668-462687 Msa1003310:intron 35.0%
! ATGTATTGATGCAATGTTCC+GGG - chr7_1:462846-462865 None:intergenic 35.0%
! GATGTATTGATGCAATGTTC+CGG - chr7_1:462847-462866 None:intergenic 35.0%
GGATGTATTGCTTATGAATG+AGG + chr7_1:463211-463230 Msa1003310:three_prime_UTR 35.0%
TTTCAATCCCATGGAAACAA+TGG - chr7_1:463255-463274 None:intergenic 35.0%
!! CATGTTTGTTTTCTCCACAA+AGG + chr7_1:463295-463314 Msa1003310:three_prime_UTR 35.0%
! CTATTGGATTGGATTTCTGC+TGG + chr7_1:461048-461067 Msa1003310:five_prime_UTR 40.0%
CTTTATACAGGTTGTTTGGC+TGG + chr7_1:461099-461118 Msa1003310:intron 40.0%
! ATGTTGTTGCTTCATGGACT+TGG + chr7_1:461255-461274 Msa1003310:five_prime_UTR 40.0%
! TGTTGTTGCTTCATGGACTT+GGG + chr7_1:461256-461275 Msa1003310:five_prime_UTR 40.0%
ATTTGTAGGATGGCCTTTGA+TGG + chr7_1:461302-461321 Msa1003310:exon 40.0%
TGTCCTGATTGTAAACTTCC+TGG + chr7_1:461329-461348 Msa1003310:CDS 40.0%
GTCCTGATTGTAAACTTCCT+GGG + chr7_1:461330-461349 Msa1003310:CDS 40.0%
TCCTGATTGTAAACTTCCTG+GGG + chr7_1:461331-461350 Msa1003310:CDS 40.0%
CTGAGCTGAAGAAAAGTAGT+AGG + chr7_1:462015-462034 Msa1003310:intron 40.0%
CTGCATGTAAAGAGCTCAAA+AGG - chr7_1:462526-462545 None:intergenic 40.0%
! CTTTTGAGCTCTTTACATGC+AGG + chr7_1:462524-462543 Msa1003310:intron 40.0%
AAAGGAGGAAGACATCAAAG+TGG - chr7_1:462822-462841 None:intergenic 40.0%
! TGTATTGATGCAATGTTCCG+GGG - chr7_1:462845-462864 None:intergenic 40.0%
! AAACTGCACATAGTGGGAAA+AGG - chr7_1:462968-462987 None:intergenic 40.0%
! TTTTCCCACTATGTGCAGTT+TGG + chr7_1:462967-462986 Msa1003310:intron 40.0%
TTTCCCACTATGTGCAGTTT+GGG + chr7_1:462968-462987 Msa1003310:intron 40.0%
! GATGGCATGTTGTTGCTTCA+TGG + chr7_1:461249-461268 Msa1003310:intron 45.0%
GATGCACAGGATGAAACATG+TGG + chr7_1:461278-461297 Msa1003310:five_prime_UTR 45.0%
ATCAGGACAGCATCCATCAA+AGG - chr7_1:461318-461337 None:intergenic 45.0%
TCCCCAGGAAGTTTACAATC+AGG - chr7_1:461335-461354 None:intergenic 45.0%
TTGACTGAGACGTGGAACAA+AGG - chr7_1:462093-462112 None:intergenic 45.0%
!! ATATCGACTTGACTGAGACG+TGG - chr7_1:462101-462120 None:intergenic 45.0%
CGTCTCAGTCAAGTCGATAT+AGG + chr7_1:462101-462120 Msa1003310:intron 45.0%
CTATCATGCAATCAGCCAAC+TGG + chr7_1:462403-462422 Msa1003310:intron 45.0%
AGGGGAACTCATTGTGTACA+AGG - chr7_1:462496-462515 None:intergenic 45.0%
TTCCCACTATGTGCAGTTTG+GGG + chr7_1:462969-462988 Msa1003310:intron 45.0%
CGTCGGGAATGGCAATTTAA+AGG + chr7_1:463074-463093 Msa1003310:CDS 45.0%
TGCGGGACTAGACAAGACAA+AGG + chr7_1:460842-460861 Msa1003310:five_prime_UTR 50.0%
TACAGGTTGTTTGGCTGGTG+TGG + chr7_1:461104-461123 Msa1003310:intron 50.0%
TTGTTTGGCTGGTGTGGTTC+TGG + chr7_1:461110-461129 Msa1003310:intron 50.0%
TTCATGGACTTGGGATGCAC+AGG + chr7_1:461265-461284 Msa1003310:five_prime_UTR 50.0%
TGATGTCTTCCTCCTTTCCC+CGG + chr7_1:462825-462844 Msa1003310:intron 50.0%
TGATGCAATGTTCCGGGGAA+AGG - chr7_1:462840-462859 None:intergenic 50.0%
CACCCCAAACTGCACATAGT+GGG - chr7_1:462974-462993 None:intergenic 50.0%
CATTGGGCAATGTGCTTGTG+AGG - chr7_1:463051-463070 None:intergenic 50.0%
CACATTGCCCAATGTGTCGT+CGG + chr7_1:463057-463076 Msa1003310:CDS 50.0%
ACATTGCCCAATGTGTCGTC+GGG + chr7_1:463058-463077 Msa1003310:CDS 50.0%
TGAGACGTGGAACAAAGGCC+TGG - chr7_1:462088-462107 None:intergenic 55.0%
TGCAATGTTCCGGGGAAAGG+AGG - chr7_1:462837-462856 None:intergenic 55.0%
GCACCCCAAACTGCACATAG+TGG - chr7_1:462975-462994 None:intergenic 55.0%
GCCATTCCCGACGACACATT+GGG - chr7_1:463067-463086 None:intergenic 55.0%
TGCCATTCCCGACGACACAT+TGG - chr7_1:463068-463087 None:intergenic 55.0%
CTGAGTGGACAGTCATCCCC+AGG - chr7_1:461350-461369 None:intergenic 60.0%
GCCCAATGTGTCGTCGGGAA+TGG + chr7_1:463063-463082 Msa1003310:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 460824 463349 460824 ID=Msa1003310;Name=Msa1003310
chr7_1 mRNA 460824 463349 460824 ID=Msa1003310-mRNA-1;Parent=Msa1003310;Name=Msa1003310-mRNA-1;_AED=0.03;_eAED=0.03;_QI=317|1|1|1|1|1|3|249|57
chr7_1 exon 460824 461079 460824 ID=Msa1003310-mRNA-1:exon:86;Parent=Msa1003310-mRNA-1
chr7_1 exon 461250 461368 461250 ID=Msa1003310-mRNA-1:exon:87;Parent=Msa1003310-mRNA-1
chr7_1 exon 462985 463349 462985 ID=Msa1003310-mRNA-1:exon:88;Parent=Msa1003310-mRNA-1
chr7_1 five_prime_UTR 460824 461079 460824 ID=Msa1003310-mRNA-1:five_prime_utr;Parent=Msa1003310-mRNA-1
chr7_1 five_prime_UTR 461250 461310 461250 ID=Msa1003310-mRNA-1:five_prime_utr;Parent=Msa1003310-mRNA-1
chr7_1 CDS 461311 461368 461311 ID=Msa1003310-mRNA-1:cds;Parent=Msa1003310-mRNA-1
chr7_1 CDS 462985 463100 462985 ID=Msa1003310-mRNA-1:cds;Parent=Msa1003310-mRNA-1
chr7_1 three_prime_UTR 463101 463349 463101 ID=Msa1003310-mRNA-1:three_prime_utr;Parent=Msa1003310-mRNA-1
Gene Sequence

>Msa1003310

ATGGCCTTTGATGGATGCTGTCCTGATTGTAAACTTCCTGGGGATGACTGTCCACTCATTTGGGGTGCATGCAATCACGCATTTCATTTACATTGTATCTTGAAATGGGTGAATTCTCAAACCTCACAAGCACATTGCCCAATGTGTCGTCGGGAATGGCAATTTAAAGGTTGA
Protein sequence

>Msa1003310

MAFDGCCPDCKLPGDDCPLIWGACNHAFHLHCILKWVNSQTSQAHCPMCRREWQFKG*