AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0483860


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0483860 MtrunA17_Chr4g0001531 100.000 57 0 0 1 57 1 57 7.78e-39 122
Msa0483860 MtrunA17_Chr7g0214441 100.000 57 0 0 1 57 1 57 7.78e-39 122
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0483860 AT3G05870.1 100.000 56 0 0 1 56 31 86 5.19e-38 120
Msa0483860 AT3G05870.2 100.000 56 0 0 1 56 31 86 5.19e-38 120
Msa0483860 AT3G05870.6 100.000 56 0 0 1 56 31 86 5.19e-38 120
Msa0483860 AT3G05870.4 100.000 56 0 0 1 56 34 89 6.68e-38 120
Msa0483860 AT3G05870.3 100.000 56 0 0 1 56 34 89 6.68e-38 120
Msa0483860 AT3G05870.5 100.000 56 0 0 1 56 34 89 6.68e-38 120
Msa0483860 AT3G05870.7 100.000 56 0 0 1 56 22 77 9.44e-38 120

Find 40 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAATTTGATCATTTCTAT+TGG 0.277487 3_4:-688858 Msa0483860:three_prime_UTR
TGAAATTAGAGTTTCTAAAT+TGG 0.278981 3_4:-688778 Msa0483860:three_prime_UTR
CGTCGGGAATGGCAATTTAA+AGG 0.290239 3_4:-688974 Msa0483860:CDS
ATTTACATTGTATCTTGAAA+TGG 0.311304 3_4:-689036 Msa0483860:CDS
CTGATAATGAAGTTAGGGTT+TGG 0.352667 3_4:+691121 None:intergenic
GATGGCATGTTGTTGCTTCA+TGG 0.372099 3_4:-690799 Msa0483860:five_prime_UTR
ATGTTGTTGCTTCATGGACT+TGG 0.375530 3_4:-690793 Msa0483860:five_prime_UTR
TGTCCTGATTGTAAACTTCC+TGG 0.394301 3_4:-690719 Msa0483860:CDS
ATACATACGTTTGAATTTGC+GGG 0.399310 3_4:-691223 Msa0483860:five_prime_UTR
AAAGAAGATTTGAATTGAAA+AGG 0.400354 3_4:+691147 None:intergenic
CTATTGGATTGGATTTCTGC+TGG 0.407759 3_4:-691000 Msa0483860:five_prime_UTR
TTTACATTGTATCTTGAAAT+GGG 0.412787 3_4:-689035 Msa0483860:CDS
TAATATCTCCATTGTTTCCA+TGG 0.415568 3_4:-688804 Msa0483860:three_prime_UTR
TATACATACGTTTGAATTTG+CGG 0.415740 3_4:-691224 Msa0483860:five_prime_UTR
ATTTGTAGGATGGCCTTTGA+TGG 0.417636 3_4:-690746 Msa0483860:exon
GCCATTCCCGACGACACATT+GGG 0.449214 3_4:+688984 None:intergenic
GTCCTGATTGTAAACTTCCT+GGG 0.462403 3_4:-690718 Msa0483860:CDS
AAACTCTAATTTCAATCCCA+TGG 0.474150 3_4:+688787 None:intergenic
ACTAGACAAGACAAAGGAAA+AGG 0.484810 3_4:-691200 Msa0483860:five_prime_UTR
ATGAAACATGTGGAATTTGT+AGG 0.488299 3_4:-690760 Msa0483860:five_prime_UTR
TTTGAAGCAAAAGCACAAAC+AGG 0.490100 3_4:+691051 None:intergenic
TGCCATTCCCGACGACACAT+TGG 0.494237 3_4:+688983 None:intergenic
GCCCAATGTGTCGTCGGGAA+TGG 0.498823 3_4:-688985 Msa0483860:CDS
TGTTGTTGCTTCATGGACTT+GGG 0.505473 3_4:-690792 Msa0483860:five_prime_UTR
AATATCTCCATTGTTTCCAT+GGG 0.512610 3_4:-688803 Msa0483860:three_prime_UTR
CACATTGCCCAATGTGTCGT+CGG 0.513658 3_4:-688991 Msa0483860:CDS
TCCCCAGGAAGTTTACAATC+AGG 0.518100 3_4:+690716 None:intergenic
CATTGGGCAATGTGCTTGTG+AGG 0.523053 3_4:+689000 None:intergenic
TTTCAATCCCATGGAAACAA+TGG 0.523838 3_4:+688796 None:intergenic
GGATGTATTGCTTATGAATG+AGG 0.540581 3_4:-688837 Msa0483860:three_prime_UTR
ACATTGCCCAATGTGTCGTC+GGG 0.540781 3_4:-688990 Msa0483860:CDS
TTCATGGACTTGGGATGCAC+AGG 0.541212 3_4:-690783 Msa0483860:five_prime_UTR
GATTTGAATTGAAAAGGAGA+AGG 0.548938 3_4:+691153 None:intergenic
TCCTGATTGTAAACTTCCTG+GGG 0.574605 3_4:-690717 Msa0483860:CDS
CTGAGTGGACAGTCATCCCC+AGG 0.578584 3_4:+690701 None:intergenic
ATCAGGACAGCATCCATCAA+AGG 0.590910 3_4:+690733 None:intergenic
CTAGACAAGACAAAGGAAAA+GGG 0.599308 3_4:-691199 Msa0483860:five_prime_UTR
TGCGGGACTAGACAAGACAA+AGG 0.697609 3_4:-691206 Msa0483860:five_prime_UTR
AACATGTGGAATTTGTAGGA+TGG 0.713439 3_4:-690756 Msa0483860:exon
GATGCACAGGATGAAACATG+TGG 0.727134 3_4:-690770 Msa0483860:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAACTAATGAATAAATATT+AGG + chr3_4:689082-689101 None:intergenic 15.0%
!! TCATTCAATAAAATAAACAA+TGG + chr3_4:689726-689745 None:intergenic 15.0%
!! TTATCTAAATGTTAAAACAA+AGG + chr3_4:689752-689771 None:intergenic 15.0%
!! AATATATTATTTGATATGTC+AGG + chr3_4:690813-690832 None:intergenic 15.0%
!! AAAGAAGATTTGAATTGAAA+AGG + chr3_4:688801-688820 None:intergenic 20.0%
!!! ATTTTCTGATAATGAAGTTA+GGG + chr3_4:688832-688851 None:intergenic 20.0%
!!! AATAAATTAAAAGCACAAAC+AGG + chr3_4:689384-689403 None:intergenic 20.0%
!! TTGTTTGTATAAAGTGAAAA+GGG + chr3_4:689555-689574 None:intergenic 20.0%
!! ATTGTTTGTATAAAGTGAAA+AGG + chr3_4:689556-689575 None:intergenic 20.0%
!! TAAAATAGTAAACTCTCATT+TGG - chr3_4:689610-689629 Msa0483860:intron 20.0%
!! AGAAGAATCAACATAAATTT+CGG + chr3_4:690116-690135 None:intergenic 20.0%
!! TACATTCAGTAAAATTAGAA+AGG + chr3_4:690465-690484 None:intergenic 20.0%
!! ATTTACATTGTATCTTGAAA+TGG - chr3_4:690909-690928 Msa0483860:intron 20.0%
!! TTTACATTGTATCTTGAAAT+GGG - chr3_4:690910-690929 Msa0483860:intron 20.0%
!!! CAATGTAATTTTGTTTTTCA+TGG - chr3_4:691010-691029 Msa0483860:five_prime_UTR 20.0%
!!! AAGTAACTAAGATCTTTTTA+AGG - chr3_4:691060-691079 Msa0483860:five_prime_UTR 20.0%
!!! AGTAACTAAGATCTTTTTAA+GGG - chr3_4:691061-691080 Msa0483860:five_prime_UTR 20.0%
!! TGTAATTTGATCATTTCTAT+TGG - chr3_4:691087-691106 Msa0483860:five_prime_UTR 20.0%
!! TGAAATTAGAGTTTCTAAAT+TGG - chr3_4:691167-691186 Msa0483860:five_prime_UTR 20.0%
!!! ATATATTTTTTCATCCTTTG+TGG + chr3_4:691209-691228 None:intergenic 20.0%
! TATACATACGTTTGAATTTG+CGG - chr3_4:688721-688740 Msa0483860:three_prime_UTR 25.0%
!!! CATTTTCTGATAATGAAGTT+AGG + chr3_4:688833-688852 None:intergenic 25.0%
! ATCTCTAACTAACTTTATAC+AGG - chr3_4:688984-689003 Msa0483860:CDS 25.0%
!!! AATATTAGGTTGATTTCACT+AGG + chr3_4:689068-689087 None:intergenic 25.0%
! TCAGTAAGTTACATTATCTT+GGG - chr3_4:689263-689282 Msa0483860:intron 25.0%
! GTCATTGAAATATGTTGAAA+TGG - chr3_4:689463-689482 Msa0483860:intron 25.0%
! TGAAAAGGGTTAATTCTTAT+TGG + chr3_4:689541-689560 None:intergenic 25.0%
! TCATGGTAACATATATATCA+CGG + chr3_4:689811-689830 None:intergenic 25.0%
! ACTAATTTCATCAATATTCC+AGG - chr3_4:689964-689983 Msa0483860:intron 25.0%
! AACATTCTTCTTAACAGAAT+AGG - chr3_4:690067-690086 Msa0483860:intron 25.0%
! AATGGATGAGATACTTTAAA+GGG + chr3_4:690166-690185 None:intergenic 25.0%
! AAATGGATGAGATACTTTAA+AGG + chr3_4:690167-690186 None:intergenic 25.0%
!!! TAAAGTATCTCATCCATTTT+TGG - chr3_4:690168-690187 Msa0483860:intron 25.0%
! TTTACCACGTCATTAATATA+TGG - chr3_4:690248-690267 Msa0483860:intron 25.0%
! TTACCACGTCATTAATATAT+GGG - chr3_4:690249-690268 Msa0483860:intron 25.0%
! ATACCTATAAACAACATTTG+TGG - chr3_4:690496-690515 Msa0483860:intron 25.0%
!! AAACCACAAATGTTGTTTAT+AGG + chr3_4:690502-690521 None:intergenic 25.0%
!! TTTATAAAGCTTTATGCTCA+AGG - chr3_4:690593-690612 Msa0483860:intron 25.0%
!!! CATGATTGAGAATTTTTCAA+GGG - chr3_4:690634-690653 Msa0483860:intron 25.0%
!!! TTTGTTTTTCATGGAAAACT+AGG - chr3_4:691019-691038 Msa0483860:five_prime_UTR 25.0%
!!! TTGTTTTTCATGGAAAACTA+GGG - chr3_4:691020-691039 Msa0483860:five_prime_UTR 25.0%
ATACATACGTTTGAATTTGC+GGG - chr3_4:688722-688741 Msa0483860:three_prime_UTR 30.0%
GATTTGAATTGAAAAGGAGA+AGG + chr3_4:688795-688814 None:intergenic 30.0%
GGTTGAATGCAAAAATGAAA+AGG + chr3_4:688876-688895 None:intergenic 30.0%
!! TGACTTAGTTTTCTCTCTAT+TGG - chr3_4:688929-688948 Msa0483860:three_prime_UTR 30.0%
! TAGTTTTCTCTCTATTGGAT+TGG - chr3_4:688934-688953 Msa0483860:three_prime_UTR 30.0%
CTAACTTTATACAGGTTGTT+TGG - chr3_4:688992-689011 Msa0483860:CDS 30.0%
CTTATCTCATCAAATGTAGA+TGG - chr3_4:689128-689147 Msa0483860:intron 30.0%
ATGAAACATGTGGAATTTGT+AGG - chr3_4:689185-689204 Msa0483860:intron 30.0%
AGATAATGTAACTTACTGAG+TGG + chr3_4:689262-689281 None:intergenic 30.0%
CTCAGTAAGTTACATTATCT+TGG - chr3_4:689262-689281 Msa0483860:intron 30.0%
ATTATCTTGGGATTTGTCTT+TGG - chr3_4:689275-689294 Msa0483860:intron 30.0%
AATTCAGCACAATAGAAAAG+TGG + chr3_4:689436-689455 None:intergenic 30.0%
!! CTTATAAGAGTGTTATGAAG+TGG - chr3_4:689587-689606 Msa0483860:intron 30.0%
TAGTAAACTCTCATTTGGAT+TGG - chr3_4:689615-689634 Msa0483860:intron 30.0%
ATGGAATTTAACAACACTTC+AGG + chr3_4:689707-689726 None:intergenic 30.0%
AACATATATATCACGGTAGT+TGG + chr3_4:689804-689823 None:intergenic 30.0%
ACAATTGCATGATAGTTTCA+TGG + chr3_4:689828-689847 None:intergenic 30.0%
AAATAGATGCTGTAAAAAGC+AGG - chr3_4:689879-689898 Msa0483860:intron 30.0%
AATAGATGCTGTAAAAAGCA+GGG - chr3_4:689880-689899 Msa0483860:intron 30.0%
! GTACTTATTGGTTATGTTGT+AGG + chr3_4:690024-690043 None:intergenic 30.0%
GAACAAAATTAGGCCAAAAA+TGG + chr3_4:690184-690203 None:intergenic 30.0%
AAACCCATATATTAATGACG+TGG + chr3_4:690255-690274 None:intergenic 30.0%
!!! GATTTTTAGTTGAAACCAGT+TGG + chr3_4:690318-690337 None:intergenic 30.0%
! AATGTTGTTTATAGGTATCC+AGG + chr3_4:690494-690513 None:intergenic 30.0%
! GCATGATTGAGAATTTTTCA+AGG - chr3_4:690633-690652 Msa0483860:intron 30.0%
TAATATCTCCATTGTTTCCA+TGG - chr3_4:691141-691160 Msa0483860:five_prime_UTR 30.0%
AATATCTCCATTGTTTCCAT+GGG - chr3_4:691142-691161 Msa0483860:five_prime_UTR 30.0%
AAACTCTAATTTCAATCCCA+TGG + chr3_4:691161-691180 None:intergenic 30.0%
ACTAGACAAGACAAAGGAAA+AGG - chr3_4:688745-688764 Msa0483860:three_prime_UTR 35.0%
CTAGACAAGACAAAGGAAAA+GGG - chr3_4:688746-688765 Msa0483860:three_prime_UTR 35.0%
! CTGATAATGAAGTTAGGGTT+TGG + chr3_4:688827-688846 None:intergenic 35.0%
! TTTGAAGCAAAAGCACAAAC+AGG + chr3_4:688897-688916 None:intergenic 35.0%
AACATGTGGAATTTGTAGGA+TGG - chr3_4:689189-689208 Msa0483860:intron 35.0%
CAATAGAAAAGTGGACAAAC+AGG + chr3_4:689427-689446 None:intergenic 35.0%
ATAGATGCTGTAAAAAGCAG+GGG - chr3_4:689881-689900 Msa0483860:intron 35.0%
CCACAAGATTGTGTACTTAT+TGG + chr3_4:690036-690055 None:intergenic 35.0%
CCAATAAGTACACAATCTTG+TGG - chr3_4:690033-690052 Msa0483860:intron 35.0%
!! CTTCTCTAAGATGACTATGT+TGG - chr3_4:690131-690150 Msa0483860:intron 35.0%
GAGTCAAGAGGAACAAAATT+AGG + chr3_4:690194-690213 None:intergenic 35.0%
TTTCTCACATAAGAGTCAAG+AGG + chr3_4:690206-690225 None:intergenic 35.0%
! CTGATTGCATGATAGTGTAA+TGG + chr3_4:690295-690314 None:intergenic 35.0%
AGCTCAAAAGGCAATAAAAG+GGG + chr3_4:690411-690430 None:intergenic 35.0%
GAGCTCAAAAGGCAATAAAA+GGG + chr3_4:690412-690431 None:intergenic 35.0%
AGAGCTCAAAAGGCAATAAA+AGG + chr3_4:690413-690432 None:intergenic 35.0%
TTACTGAATGTAGATAAGCC+TGG - chr3_4:690473-690492 Msa0483860:intron 35.0%
TGTGTTATCACTGACATTCA+TGG - chr3_4:690564-690583 Msa0483860:intron 35.0%
GTGTTATCACTGACATTCAT+GGG - chr3_4:690565-690584 Msa0483860:intron 35.0%
! ATGTATTGATGCAATGTTCC+GGG + chr3_4:690743-690762 None:intergenic 35.0%
! GATGTATTGATGCAATGTTC+CGG + chr3_4:690744-690763 None:intergenic 35.0%
GGATGTATTGCTTATGAATG+AGG - chr3_4:691108-691127 Msa0483860:five_prime_UTR 35.0%
TTTCAATCCCATGGAAACAA+TGG + chr3_4:691152-691171 None:intergenic 35.0%
!! CATGTTTGTTTTCTCCACAA+AGG - chr3_4:691192-691211 Msa0483860:five_prime_UTR 35.0%
! CTATTGGATTGGATTTCTGC+TGG - chr3_4:688945-688964 Msa0483860:three_prime_UTR 40.0%
CTTTATACAGGTTGTTTGGC+TGG - chr3_4:688996-689015 Msa0483860:CDS 40.0%
! ATGTTGTTGCTTCATGGACT+TGG - chr3_4:689152-689171 Msa0483860:intron 40.0%
! TGTTGTTGCTTCATGGACTT+GGG - chr3_4:689153-689172 Msa0483860:intron 40.0%
ATTTGTAGGATGGCCTTTGA+TGG - chr3_4:689199-689218 Msa0483860:intron 40.0%
TGTCCTGATTGTAAACTTCC+TGG - chr3_4:689226-689245 Msa0483860:intron 40.0%
GTCCTGATTGTAAACTTCCT+GGG - chr3_4:689227-689246 Msa0483860:intron 40.0%
TCCTGATTGTAAACTTCCTG+GGG - chr3_4:689228-689247 Msa0483860:intron 40.0%
CTGAGCTGAAGAAAAGTAGT+AGG - chr3_4:689912-689931 Msa0483860:intron 40.0%
CTGCATGTAAAGAGCTCAAA+AGG + chr3_4:690423-690442 None:intergenic 40.0%
! CTTTTGAGCTCTTTACATGC+AGG - chr3_4:690421-690440 Msa0483860:intron 40.0%
AAAGGAGGAAGACATCAAAG+TGG + chr3_4:690719-690738 None:intergenic 40.0%
! TGTATTGATGCAATGTTCCG+GGG + chr3_4:690742-690761 None:intergenic 40.0%
! AAACTGCACATAGTGGGAAA+AGG + chr3_4:690865-690884 None:intergenic 40.0%
! TTTTCCCACTATGTGCAGTT+TGG - chr3_4:690864-690883 Msa0483860:intron 40.0%
TTTCCCACTATGTGCAGTTT+GGG - chr3_4:690865-690884 Msa0483860:intron 40.0%
! GATGGCATGTTGTTGCTTCA+TGG - chr3_4:689146-689165 Msa0483860:intron 45.0%
GATGCACAGGATGAAACATG+TGG - chr3_4:689175-689194 Msa0483860:intron 45.0%
ATCAGGACAGCATCCATCAA+AGG + chr3_4:689215-689234 None:intergenic 45.0%
TCCCCAGGAAGTTTACAATC+AGG + chr3_4:689232-689251 None:intergenic 45.0%
TTGACTGAGACGTGGAACAA+AGG + chr3_4:689990-690009 None:intergenic 45.0%
!! ATATCGACTTGACTGAGACG+TGG + chr3_4:689998-690017 None:intergenic 45.0%
CGTCTCAGTCAAGTCGATAT+AGG - chr3_4:689998-690017 Msa0483860:intron 45.0%
CTATCATGCAATCAGCCAAC+TGG - chr3_4:690300-690319 Msa0483860:intron 45.0%
AGGGGAACTCATTGTGTACA+AGG + chr3_4:690393-690412 None:intergenic 45.0%
TTCCCACTATGTGCAGTTTG+GGG - chr3_4:690866-690885 Msa0483860:intron 45.0%
CGTCGGGAATGGCAATTTAA+AGG - chr3_4:690971-690990 Msa0483860:intron 45.0%
TGCGGGACTAGACAAGACAA+AGG - chr3_4:688739-688758 Msa0483860:three_prime_UTR 50.0%
TACAGGTTGTTTGGCTGGTG+TGG - chr3_4:689001-689020 Msa0483860:CDS 50.0%
TTGTTTGGCTGGTGTGGTTC+TGG - chr3_4:689007-689026 Msa0483860:CDS 50.0%
TTCATGGACTTGGGATGCAC+AGG - chr3_4:689162-689181 Msa0483860:intron 50.0%
TGATGTCTTCCTCCTTTCCC+CGG - chr3_4:690722-690741 Msa0483860:CDS 50.0%
TGATGCAATGTTCCGGGGAA+AGG + chr3_4:690737-690756 None:intergenic 50.0%
CACCCCAAACTGCACATAGT+GGG + chr3_4:690871-690890 None:intergenic 50.0%
CATTGGGCAATGTGCTTGTG+AGG + chr3_4:690948-690967 None:intergenic 50.0%
CACATTGCCCAATGTGTCGT+CGG - chr3_4:690954-690973 Msa0483860:intron 50.0%
ACATTGCCCAATGTGTCGTC+GGG - chr3_4:690955-690974 Msa0483860:intron 50.0%
TGAGACGTGGAACAAAGGCC+TGG + chr3_4:689985-690004 None:intergenic 55.0%
TGCAATGTTCCGGGGAAAGG+AGG + chr3_4:690734-690753 None:intergenic 55.0%
GCACCCCAAACTGCACATAG+TGG + chr3_4:690872-690891 None:intergenic 55.0%
GCCATTCCCGACGACACATT+GGG + chr3_4:690964-690983 None:intergenic 55.0%
TGCCATTCCCGACGACACAT+TGG + chr3_4:690965-690984 None:intergenic 55.0%
CTGAGTGGACAGTCATCCCC+AGG + chr3_4:689247-689266 None:intergenic 60.0%
GCCCAATGTGTCGTCGGGAA+TGG - chr3_4:690960-690979 Msa0483860:intron 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 688721 691246 688721 ID=Msa0483860;Name=Msa0483860
chr3_4 mRNA 688721 691246 688721 ID=Msa0483860-mRNA-1;Parent=Msa0483860;Name=Msa0483860-mRNA-1;_AED=0.03;_eAED=0.03;_QI=317|1|1|1|0.5|0.66|3|249|57
chr3_4 exon 688721 689085 688721 ID=Msa0483860-mRNA-1:exon:237;Parent=Msa0483860-mRNA-1
chr3_4 exon 690702 690820 690702 ID=Msa0483860-mRNA-1:exon:236;Parent=Msa0483860-mRNA-1
chr3_4 exon 690991 691246 690991 ID=Msa0483860-mRNA-1:exon:235;Parent=Msa0483860-mRNA-1
chr3_4 five_prime_UTR 690991 691246 690991 ID=Msa0483860-mRNA-1:five_prime_utr;Parent=Msa0483860-mRNA-1
chr3_4 five_prime_UTR 690760 690820 690760 ID=Msa0483860-mRNA-1:five_prime_utr;Parent=Msa0483860-mRNA-1
chr3_4 CDS 690702 690759 690702 ID=Msa0483860-mRNA-1:cds;Parent=Msa0483860-mRNA-1
chr3_4 CDS 688970 689085 688970 ID=Msa0483860-mRNA-1:cds;Parent=Msa0483860-mRNA-1
chr3_4 three_prime_UTR 688721 688969 688721 ID=Msa0483860-mRNA-1:three_prime_utr;Parent=Msa0483860-mRNA-1
Gene Sequence

>Msa0483860

ATGGCCTTTGATGGATGCTGTCCTGATTGTAAACTTCCTGGGGATGACTGTCCACTCATTTGGGGTGCATGCAATCACGCATTTCATTTACATTGTATCTTGAAATGGGTGAATTCTCAAACCTCACAAGCACATTGCCCAATGTGTCGTCGGGAATGGCAATTTAAAGGTTGA

Protein sequence

>Msa0483860

MAFDGCCPDCKLPGDDCPLIWGACNHAFHLHCILKWVNSQTSQAHCPMCRREWQFKG*