AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1419800


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1419800 MtrunA17_Chr7g0266171 70.175 228 12 2 1 172 1 228 3.61e-96 278
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1419800 AT3G52760.1 56.250 160 59 3 1 149 1 160 5.07e-54 172
Msa1419800 AT2G36300.1 54.545 165 49 5 1 147 1 157 8.65e-47 153

Find 61 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCACAGGGTGGTTTCTT+TGG 0.102268 tig0024727:+74045 Msa1419800:intron
TTCCACAGGGTGGTTTCTTT+GGG 0.249145 tig0024727:+74046 Msa1419800:intron
GGAACCACCACCGCCGATTC+CGG 0.282867 tig0024727:-73669 None:intergenic
GAACCACCACCGCCGATTCC+GGG 0.362735 tig0024727:-73668 None:intergenic
GTATTCATCCGGATCAAATC+TGG 0.368693 tig0024727:+73732 Msa1419800:CDS
AACGCCATATAGAGAAGAAT+TGG 0.369820 tig0024727:-73827 None:intergenic
TGGTCCAATTCTTCTCTATA+TGG 0.369922 tig0024727:+73823 Msa1419800:CDS
CGGTATGGTTCACGCGAAAC+GGG 0.383392 tig0024727:-73778 None:intergenic
CTCCTCGTCGTCGAAGTTAA+CGG 0.422501 tig0024727:-73690 None:intergenic
TTTGGTTGGAACGGTGGTAT+TGG 0.423341 tig0024727:-73569 None:intergenic
ATGAAAGGGATGCCGGAGTT+TGG 0.440296 tig0024727:-73587 None:intergenic
ACGGTATGGTTCACGCGAAA+CGG 0.443655 tig0024727:-73779 None:intergenic
ATCTACTCTGGTCCCGGAAT+CGG 0.456452 tig0024727:+73656 Msa1419800:CDS
CTTGTGGTTTCTCTTACTGA+TGG 0.457923 tig0024727:+74120 Msa1419800:CDS
GGTCCCGGAATCGGCGGTGG+TGG 0.458366 tig0024727:+73665 Msa1419800:CDS
TGGACCGGAGGAGGTGGAAA+CGG 0.462451 tig0024727:-73633 None:intergenic
CACAAAGACGCTGATCTCTC+TGG 0.477534 tig0024727:+73803 Msa1419800:CDS
GGAGGAGGTGGAAACGGCGG+CGG 0.482053 tig0024727:-73627 None:intergenic
CCGACGGAGGCGAAGGATAC+CGG 0.487942 tig0024727:-73509 None:intergenic
ATTCCGGGACCAGAGTAGAT+TGG 0.488100 tig0024727:-73653 None:intergenic
TGGGCTACTAGAGCTTCAAC+GGG 0.501360 tig0024727:+74096 Msa1419800:CDS
CGATGTCAAATGACATGAAA+GGG 0.504894 tig0024727:-73601 None:intergenic
CCGGTATCCTTCGCCTCCGT+CGG 0.505190 tig0024727:+73509 Msa1419800:CDS
TCTGGTCCCGGAATCGGCGG+TGG 0.508217 tig0024727:+73662 Msa1419800:CDS
GTAGATTGGACCGGAGGAGG+TGG 0.509987 tig0024727:-73639 None:intergenic
GCCGCCGTTTCCACCTCCTC+CGG 0.518829 tig0024727:+73629 Msa1419800:CDS
ATGCCGGAGTTTGGTTGGAA+CGG 0.525630 tig0024727:-73578 None:intergenic
TAGAGCTTCAACGGGGCTTG+TGG 0.525930 tig0024727:+74104 Msa1419800:CDS
GGGGTTACCGACGGAGGCGA+AGG 0.531653 tig0024727:-73516 None:intergenic
AAGGGATGCCGGAGTTTGGT+TGG 0.532020 tig0024727:-73583 None:intergenic
CGACGAGCTCGGTATTCATC+CGG 0.535700 tig0024727:+73721 Msa1419800:CDS
ATACCGAGCTCGTCGAGAAG+AGG 0.537974 tig0024727:-73713 None:intergenic
GGTCCAATCTACTCTGGTCC+CGG 0.538685 tig0024727:+73650 Msa1419800:CDS
CAGCGTCTTTGTGAACGGTA+TGG 0.542374 tig0024727:-73793 None:intergenic
CCTTTCATGTCATTTGACAT+CGG 0.544673 tig0024727:+73602 Msa1419800:CDS
TTGTCCGGCGGGGTTACCGA+CGG 0.546984 tig0024727:-73525 None:intergenic
ATGGGCTACTAGAGCTTCAA+CGG 0.549815 tig0024727:+74095 Msa1419800:CDS
TACTCTGGTCCCGGAATCGG+CGG 0.561190 tig0024727:+73659 Msa1419800:CDS
GAGCCTCTTCTCGACGAGCT+CGG 0.565975 tig0024727:+73710 Msa1419800:CDS
AAATGACATGAAAGGGATGC+CGG 0.570013 tig0024727:-73594 None:intergenic
GCCTCCGTCGGTAACCCCGC+CGG 0.572655 tig0024727:+73521 Msa1419800:CDS
TCCTCCGGTCCAATCTACTC+TGG 0.574768 tig0024727:+73644 Msa1419800:CDS
AGAGTAGATTGGACCGGAGG+AGG 0.576450 tig0024727:-73642 None:intergenic
CCGGAGTTTGGTTGGAACGG+TGG 0.579512 tig0024727:-73575 None:intergenic
CCACCGTTCCAACCAAACTC+CGG 0.614609 tig0024727:+73575 Msa1419800:CDS
GGGACCAGAGTAGATTGGAC+CGG 0.616175 tig0024727:-73648 None:intergenic
GAGATCAGCGTCTTTGTGAA+CGG 0.616493 tig0024727:-73798 None:intergenic
GGAACGGTGGTATTGGTACA+CGG 0.619478 tig0024727:-73562 None:intergenic
CCGATGTCAAATGACATGAA+AGG 0.627556 tig0024727:-73602 None:intergenic
GAAAGAATTCAAAGTCCCAC+CGG 0.628112 tig0024727:+73490 Msa1419800:CDS
TCCGGCGGGGTTACCGACGG+AGG 0.630208 tig0024727:-73522 None:intergenic
ACCAGAGTAGATTGGACCGG+AGG 0.633247 tig0024727:-73645 None:intergenic
ACGGAGGCGAAGGATACCGG+TGG 0.636258 tig0024727:-73506 None:intergenic
TTCCGTTAACTTCGACGACG+AGG 0.637706 tig0024727:+73688 Msa1419800:CDS
ACCGGAGGAGGTGGAAACGG+CGG 0.648522 tig0024727:-73630 None:intergenic
AATCTGGAGCAAAATCAGAT+CGG 0.650716 tig0024727:+73748 Msa1419800:CDS
CGGAGGCGAAGGATACCGGT+GGG 0.687481 tig0024727:-73505 None:intergenic
ATGGCTGTGATGAACATCGT+GGG 0.690522 tig0024727:+74139 Msa1419800:CDS
GATGGCTGTGATGAACATCG+TGG 0.761743 tig0024727:+74138 Msa1419800:CDS
GGGCTACTAGAGCTTCAACG+GGG 0.801660 tig0024727:+74097 Msa1419800:CDS
ACGGTGGTATTGGTACACGG+CGG 0.809815 tig0024727:-73559 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCACACCGAATTGAATTTTT+CGG - tig0024727:73873-73892 None:intergenic 30.0%
!!! ATTCAATTCGGTGTGATTTT+AGG + tig0024727:73877-73896 Msa1419800:intron 30.0%
CGATGTCAAATGACATGAAA+GGG - tig0024727:73604-73623 None:intergenic 35.0%
! CCTTTCATGTCATTTGACAT+CGG + tig0024727:73602-73621 Msa1419800:CDS 35.0%
! ATTTTGCTCCAGATTTGATC+CGG - tig0024727:73743-73762 None:intergenic 35.0%
! AATCTGGAGCAAAATCAGAT+CGG + tig0024727:73748-73767 Msa1419800:CDS 35.0%
TGGTCCAATTCTTCTCTATA+TGG + tig0024727:73823-73842 Msa1419800:CDS 35.0%
AACGCCATATAGAGAAGAAT+TGG - tig0024727:73830-73849 None:intergenic 35.0%
ACTCGCCGAAAAATTCAATT+CGG + tig0024727:73865-73884 Msa1419800:CDS 35.0%
!!! AATTCGGTGTGATTTTAGGT+TGG + tig0024727:73881-73900 Msa1419800:intron 35.0%
CATAGAATAACCAACAACAC+TGG - tig0024727:73989-74008 None:intergenic 35.0%
!!! AATATCGCTTTTTCTTCCAC+AGG + tig0024727:74032-74051 Msa1419800:intron 35.0%
!!! ATATCGCTTTTTCTTCCACA+GGG + tig0024727:74033-74052 Msa1419800:intron 35.0%
! TCGCTTCGATTTTTGTTCTA+TGG + tig0024727:74076-74095 Msa1419800:CDS 35.0%
! CGCTTCGATTTTTGTTCTAT+GGG + tig0024727:74077-74096 Msa1419800:CDS 35.0%
GAAAGAATTCAAAGTCCCAC+CGG + tig0024727:73490-73509 Msa1419800:CDS 40.0%
!! TTTTGAAGATTTTGTCCGGC+GGG - tig0024727:73539-73558 None:intergenic 40.0%
!!! GTTTTGAAGATTTTGTCCGG+CGG - tig0024727:73540-73559 None:intergenic 40.0%
!!! GCGGTTTTGAAGATTTTGTC+CGG - tig0024727:73543-73562 None:intergenic 40.0%
AAATGACATGAAAGGGATGC+CGG - tig0024727:73597-73616 None:intergenic 40.0%
CCGATGTCAAATGACATGAA+AGG - tig0024727:73605-73624 None:intergenic 40.0%
GTATTCATCCGGATCAAATC+TGG + tig0024727:73732-73751 Msa1419800:CDS 40.0%
!!! TGTGATTTTAGGTTGGATCG+TGG + tig0024727:73888-73907 Msa1419800:intron 40.0%
!! CGTTGTTTTCAACATGCTTG+CGG + tig0024727:73930-73949 Msa1419800:intron 40.0%
!! GTTGTTTTCAACATGCTTGC+GGG + tig0024727:73931-73950 Msa1419800:intron 40.0%
CATACTTGTACCAGTGTTGT+TGG + tig0024727:73976-73995 Msa1419800:intron 40.0%
TGGTTATTCTATGTTGCCAG+TGG + tig0024727:73996-74015 Msa1419800:intron 40.0%
ATTGCAGAGAAAATCACCAC+TGG - tig0024727:74015-74034 None:intergenic 40.0%
CTTGTGGTTTCTCTTACTGA+TGG + tig0024727:74120-74139 Msa1419800:CDS 40.0%
! TTTGAAGATTTTGTCCGGCG+GGG - tig0024727:73538-73557 None:intergenic 45.0%
TTTGGTTGGAACGGTGGTAT+TGG - tig0024727:73572-73591 None:intergenic 45.0%
GAGATCAGCGTCTTTGTGAA+CGG - tig0024727:73801-73820 None:intergenic 45.0%
TTCCACAGGGTGGTTTCTTT+GGG + tig0024727:74046-74065 Msa1419800:intron 45.0%
! ATGGGCTACTAGAGCTTCAA+CGG + tig0024727:74095-74114 Msa1419800:CDS 45.0%
ATGGCTGTGATGAACATCGT+GGG + tig0024727:74139-74158 Msa1419800:CDS 45.0%
!! GGAACGGTGGTATTGGTACA+CGG - tig0024727:73565-73584 None:intergenic 50.0%
ATGCCGGAGTTTGGTTGGAA+CGG - tig0024727:73581-73600 None:intergenic 50.0%
ATGAAAGGGATGCCGGAGTT+TGG - tig0024727:73590-73609 None:intergenic 50.0%
ATTCCGGGACCAGAGTAGAT+TGG - tig0024727:73656-73675 None:intergenic 50.0%
ATCTACTCTGGTCCCGGAAT+CGG + tig0024727:73656-73675 Msa1419800:CDS 50.0%
TTCCGTTAACTTCGACGACG+AGG + tig0024727:73688-73707 Msa1419800:CDS 50.0%
CTCCTCGTCGTCGAAGTTAA+CGG - tig0024727:73693-73712 None:intergenic 50.0%
ACGGTATGGTTCACGCGAAA+CGG - tig0024727:73782-73801 None:intergenic 50.0%
CAGCGTCTTTGTGAACGGTA+TGG - tig0024727:73796-73815 None:intergenic 50.0%
CACAAAGACGCTGATCTCTC+TGG + tig0024727:73803-73822 Msa1419800:CDS 50.0%
TGCGGGTCGAACTGGAAATT+TGG + tig0024727:73948-73967 Msa1419800:intron 50.0%
!!! TCGCTTTTTCTTCCACAGGG+TGG + tig0024727:74036-74055 Msa1419800:intron 50.0%
CTTCCACAGGGTGGTTTCTT+TGG + tig0024727:74045-74064 Msa1419800:intron 50.0%
TGGGCTACTAGAGCTTCAAC+GGG + tig0024727:74096-74115 Msa1419800:CDS 50.0%
GATGGCTGTGATGAACATCG+TGG + tig0024727:74138-74157 Msa1419800:CDS 50.0%
!! ACGGTGGTATTGGTACACGG+CGG - tig0024727:73562-73581 None:intergenic 55.0%
CCACCGTTCCAACCAAACTC+CGG + tig0024727:73575-73594 Msa1419800:CDS 55.0%
AAGGGATGCCGGAGTTTGGT+TGG - tig0024727:73586-73605 None:intergenic 55.0%
AGAGTAGATTGGACCGGAGG+AGG - tig0024727:73645-73664 None:intergenic 55.0%
TCCTCCGGTCCAATCTACTC+TGG + tig0024727:73644-73663 Msa1419800:CDS 55.0%
ACCAGAGTAGATTGGACCGG+AGG - tig0024727:73648-73667 None:intergenic 55.0%
GGGACCAGAGTAGATTGGAC+CGG - tig0024727:73651-73670 None:intergenic 55.0%
GGTCCAATCTACTCTGGTCC+CGG + tig0024727:73650-73669 Msa1419800:CDS 55.0%
ATACCGAGCTCGTCGAGAAG+AGG - tig0024727:73716-73735 None:intergenic 55.0%
CGACGAGCTCGGTATTCATC+CGG + tig0024727:73721-73740 Msa1419800:CDS 55.0%
CGGTATGGTTCACGCGAAAC+GGG - tig0024727:73781-73800 None:intergenic 55.0%
AACATGCTTGCGGGTCGAAC+TGG + tig0024727:73940-73959 Msa1419800:intron 55.0%
AGCCCAAAGAAACCACCCTG+TGG - tig0024727:74051-74070 None:intergenic 55.0%
GGGCTACTAGAGCTTCAACG+GGG + tig0024727:74097-74116 Msa1419800:CDS 55.0%
TAGAGCTTCAACGGGGCTTG+TGG + tig0024727:74104-74123 Msa1419800:CDS 55.0%
CCGGAGTTTGGTTGGAACGG+TGG - tig0024727:73578-73597 None:intergenic 60.0%
TGGACCGGAGGAGGTGGAAA+CGG - tig0024727:73636-73655 None:intergenic 60.0%
GTAGATTGGACCGGAGGAGG+TGG - tig0024727:73642-73661 None:intergenic 60.0%
TACTCTGGTCCCGGAATCGG+CGG + tig0024727:73659-73678 Msa1419800:CDS 60.0%
GAGCCTCTTCTCGACGAGCT+CGG + tig0024727:73710-73729 Msa1419800:CDS 60.0%
CGGAGGCGAAGGATACCGGT+GGG - tig0024727:73508-73527 None:intergenic 65.0%
ACGGAGGCGAAGGATACCGG+TGG - tig0024727:73509-73528 None:intergenic 65.0%
CCGACGGAGGCGAAGGATAC+CGG - tig0024727:73512-73531 None:intergenic 65.0%
CCGGTATCCTTCGCCTCCGT+CGG + tig0024727:73509-73528 Msa1419800:CDS 65.0%
TTGTCCGGCGGGGTTACCGA+CGG - tig0024727:73528-73547 None:intergenic 65.0%
ACCGGAGGAGGTGGAAACGG+CGG - tig0024727:73633-73652 None:intergenic 65.0%
!! GAACCACCACCGCCGATTCC+GGG - tig0024727:73671-73690 None:intergenic 65.0%
!! GGAACCACCACCGCCGATTC+CGG - tig0024727:73672-73691 None:intergenic 65.0%
GGGGTTACCGACGGAGGCGA+AGG - tig0024727:73519-73538 None:intergenic 70.0%
GGAGGAGGTGGAAACGGCGG+CGG - tig0024727:73630-73649 None:intergenic 70.0%
GCCGCCGTTTCCACCTCCTC+CGG + tig0024727:73629-73648 Msa1419800:CDS 70.0%
TCTGGTCCCGGAATCGGCGG+TGG + tig0024727:73662-73681 Msa1419800:CDS 70.0%
GCCTCCGTCGGTAACCCCGC+CGG + tig0024727:73521-73540 Msa1419800:CDS 75.0%
TCCGGCGGGGTTACCGACGG+AGG - tig0024727:73525-73544 None:intergenic 75.0%
GGTCCCGGAATCGGCGGTGG+TGG + tig0024727:73665-73684 Msa1419800:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0024727 gene 73485 74167 73485 ID=Msa1419800;Name=Msa1419800
tig0024727 mRNA 73485 74167 73485 ID=Msa1419800-mRNA-1;Parent=Msa1419800;Name=Msa1419800-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0|0|1|1|1|2|0|172
tig0024727 exon 73485 73886 73485 ID=Msa1419800-mRNA-1:exon:1057;Parent=Msa1419800-mRNA-1
tig0024727 exon 74054 74167 74054 ID=Msa1419800-mRNA-1:exon:1058;Parent=Msa1419800-mRNA-1
tig0024727 CDS 73485 73886 73485 ID=Msa1419800-mRNA-1:cds;Parent=Msa1419800-mRNA-1
tig0024727 CDS 74054 74167 74054 ID=Msa1419800-mRNA-1:cds;Parent=Msa1419800-mRNA-1
Gene Sequence

>Msa1419800

ATGGAGAAAGAATTCAAAGTCCCACCGGTATCCTTCGCCTCCGTCGGTAACCCCGCCGGACAAAATCTTCAAAACCGCCGTGTACCAATACCACCGTTCCAACCAAACTCCGGCATCCCTTTCATGTCATTTGACATCGGCTCCGCCGCCGTTTCCACCTCCTCCGGTCCAATCTACTCTGGTCCCGGAATCGGCGGTGGTGGTTCCGTTAACTTCGACGACGAGGAGCCTCTTCTCGACGAGCTCGGTATTCATCCGGATCAAATCTGGAGCAAAATCAGATCGGTTCTTAACCCGTTTCGCGTGAACCATACCGTTCACAAAGACGCTGATCTCTCTGGTCCAATTCTTCTCTATATGGCGTTTTCTCTCTTTCAATTACTCGCCGAAAAATTCAATTCGGGTGGTTTCTTTGGGCTTGCTATCGCTTCGATTTTTGTTCTATGGGCTACTAGAGCTTCAACGGGGCTTGTGGTTTCTCTTACTGATGGCTGTGATGAACATCGTGGGTTGATA

Protein sequence

>Msa1419800

MEKEFKVPPVSFASVGNPAGQNLQNRRVPIPPFQPNSGIPFMSFDIGSAAVSTSSGPIYSGPGIGGGGSVNFDDEEPLLDELGIHPDQIWSKIRSVLNPFRVNHTVHKDADLSGPILLYMAFSLFQLLAEKFNSGGFFGLAIASIFVLWATRASTGLVVSLTDGCDEHRGLI