Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1131020 | AES82811.1 | 95.192 | 104 | 4 | 1 | 1 | 103 | 1 | 104 | 1.60e-64 | 200 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1131020 | G7L533 | 95.192 | 104 | 4 | 1 | 1 | 103 | 1 | 104 | 7.63e-65 | 200 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017120 | Msa1131020 | 0.809250 | 2.076498e-50 | -8.615850e-47 |
| Msa0630970 | Msa1131020 | 0.801491 | 8.782946e-49 | -8.615850e-47 |
| Msa0638690 | Msa1131020 | 0.802836 | 4.642965e-49 | -8.615850e-47 |
| Msa0642910 | Msa1131020 | 0.831082 | 2.041978e-55 | -8.615850e-47 |
| Msa0669200 | Msa1131020 | 0.807802 | 4.230715e-50 | -8.615850e-47 |
| Msa0678890 | Msa1131020 | 0.821768 | 3.386845e-53 | -8.615850e-47 |
| Msa0690560 | Msa1131020 | 0.820524 | 6.553927e-53 | -8.615850e-47 |
| Msa0720270 | Msa1131020 | 0.804533 | 2.063723e-49 | -8.615850e-47 |
| Msa0782330 | Msa1131020 | 0.801641 | 8.181058e-49 | -8.615850e-47 |
| Msa0803130 | Msa1131020 | 0.823233 | 1.546844e-53 | -8.615850e-47 |
| Msa0810020 | Msa1131020 | 0.800995 | 1.109431e-48 | -8.615850e-47 |
| Msa0841490 | Msa1131020 | 0.826083 | 3.295729e-54 | -8.615850e-47 |
| Msa0143080 | Msa1131020 | 0.815069 | 1.116580e-51 | -8.615850e-47 |
| Msa0157020 | Msa1131020 | 0.810022 | 1.416803e-50 | -8.615850e-47 |
| Msa0207180 | Msa1131020 | 0.819060 | 1.416183e-52 | -8.615850e-47 |
| Msa0236980 | Msa1131020 | 0.802558 | 5.298351e-49 | -8.615850e-47 |
| Msa0370600 | Msa1131020 | 0.807439 | 5.051859e-50 | -8.615850e-47 |
| Msa0408640 | Msa1131020 | 0.825837 | 3.770504e-54 | -8.615850e-47 |
| Msa0418200 | Msa1131020 | 0.808504 | 2.998312e-50 | -8.615850e-47 |
| Msa1106810 | Msa1131020 | 0.803269 | 3.777912e-49 | -8.615850e-47 |
| Msa0277530 | Msa1131020 | 0.822764 | 1.988845e-53 | -8.615850e-47 |
| Msa0297920 | Msa1131020 | 0.804538 | 2.058808e-49 | -8.615850e-47 |
| Msa0315480 | Msa1131020 | 0.819226 | 1.297724e-52 | -8.615850e-47 |
| Msa0328210 | Msa1131020 | 0.807211 | 5.646534e-50 | -8.615850e-47 |
| Msa0361260 | Msa1131020 | 0.827601 | 1.429743e-54 | -8.615850e-47 |
| Msa1123880 | Msa1131020 | 0.802139 | 6.463798e-49 | -8.615850e-47 |
| Msa1130690 | Msa1131020 | 0.957027 | 7.284097e-115 | -8.615850e-47 |
| Msa1131020 | Msa1254190 | 0.803604 | 3.220695e-49 | -8.615850e-47 |
| Msa1131020 | Msa1355400 | 0.807835 | 4.162452e-50 | -8.615850e-47 |
| Msa1131020 | Msa1365020 | 0.802646 | 5.081829e-49 | -8.615850e-47 |
| Msa1131020 | Msa1367410 | 0.815490 | 9.001337e-52 | -8.615850e-47 |
| Msa1131020 | Msa1420060 | 0.814797 | 1.282936e-51 | -8.615850e-47 |
| Msa1131020 | Msa1425060 | 0.975613 | 2.830605e-140 | -8.615850e-47 |
| Msa1131020 | Msa1444450 | 0.814768 | 1.302419e-51 | -8.615850e-47 |
| Msa0485420 | Msa1131020 | 0.802766 | 4.800231e-49 | -8.615850e-47 |
| Msa0497420 | Msa1131020 | 0.805656 | 1.201251e-49 | -8.615850e-47 |
| Msa0524210 | Msa1131020 | 0.808515 | 2.982662e-50 | -8.615850e-47 |
| Msa0547050 | Msa1131020 | 0.828673 | 7.889512e-55 | -8.615850e-47 |
| Msa0590660 | Msa1131020 | 0.803799 | 2.933717e-49 | -8.615850e-47 |
| Msa0601780 | Msa1131020 | 0.802838 | 4.639164e-49 | -8.615850e-47 |
| Msa0872310 | Msa1131020 | 0.812283 | 4.583520e-51 | -8.615850e-47 |
| Msa0877420 | Msa1131020 | 0.823811 | 1.133048e-53 | -8.615850e-47 |
| Msa0905550 | Msa1131020 | 0.805308 | 1.421044e-49 | -8.615850e-47 |
| Msa0922540 | Msa1131020 | 0.800342 | 1.507370e-48 | -8.615850e-47 |
| Msa0931610 | Msa1131020 | 0.800056 | 1.723955e-48 | -8.615850e-47 |
| Msa0937550 | Msa1131020 | 0.822624 | 2.144309e-53 | -8.615850e-47 |
| Msa0942730 | Msa1131020 | 0.821033 | 5.006119e-53 | -8.615850e-47 |
| Msa0958020 | Msa1131020 | 0.807612 | 4.641655e-50 | -8.615850e-47 |
| Msa0974750 | Msa1131020 | 0.805738 | 1.154710e-49 | -8.615850e-47 |
| Msa0979550 | Msa1131020 | 0.809597 | 1.748750e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1131020 | MtrunA17_Chr7g0276141 | 95.192 | 104 | 4 | 1 | 1 | 103 | 1 | 104 | 1.47e-68 | 200 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCAAAGGGGCTGAGTTTA+AGG | 0.194747 | 7_3:+83340021 | Msa1131020:CDS |
| GGCCAACTCAAGGATTTAAT+AGG | 0.281462 | 7_3:+83339873 | Msa1131020:CDS |
| AACAATGACAATTTAGAATT+AGG | 0.285252 | 7_3:+83339909 | Msa1131020:CDS |
| CTGTGGTAGTTCACCGCATT+CGG | 0.308570 | 7_3:+83339814 | Msa1131020:CDS |
| TCTTCGCTATGTTCTTGAAT+TGG | 0.381899 | 7_3:-83340044 | None:intergenic |
| TAAATCCTTGAGTTGGCCTT+TGG | 0.387136 | 7_3:-83339868 | None:intergenic |
| AAAATGGGTAATGCTTGCTT+TGG | 0.394791 | 7_3:+83339759 | None:intergenic |
| GCCAACTCAAGGATTTAATA+GGG | 0.437318 | 7_3:+83339874 | Msa1131020:CDS |
| TGTGGTAGTTCACCGCATTC+GGG | 0.441159 | 7_3:+83339815 | Msa1131020:CDS |
| ACGAGCACGAAGCCTCCCTT+TGG | 0.474923 | 7_3:-83339955 | None:intergenic |
| TCCCTATTAAATCCTTGAGT+TGG | 0.476466 | 7_3:-83339875 | None:intergenic |
| AGCATTCTTGAACGATCAAA+TGG | 0.501702 | 7_3:-83339932 | None:intergenic |
| GGATCAAGCCGTGACGATCA+AGG | 0.532320 | 7_3:+83339836 | Msa1131020:CDS |
| CGAATGCGGTGAACTACCAC+AGG | 0.532604 | 7_3:-83339813 | None:intergenic |
| ATCGTTCAAGAATGCTCCAA+AGG | 0.558651 | 7_3:+83339939 | Msa1131020:CDS |
| CGTGTTGTAACTGCTGCAGT+TGG | 0.558826 | 7_3:+83339975 | Msa1131020:CDS |
| CAAGGATTTAATAGGGAAGG+TGG | 0.561353 | 7_3:+83339881 | Msa1131020:CDS |
| TCGTTCAAGAATGCTCCAAA+GGG | 0.578470 | 7_3:+83339940 | Msa1131020:CDS |
| CATTCTCACCTTGATCGTCA+CGG | 0.582038 | 7_3:-83339844 | None:intergenic |
| GAAGATGAGCAACAAGGGAG+TGG | 0.588540 | 7_3:+83339785 | Msa1131020:CDS |
| GGAAAGAAGATGAGCAACAA+GGG | 0.598826 | 7_3:+83339780 | Msa1131020:CDS |
| TGGAAAGAAGATGAGCAACA+AGG | 0.599291 | 7_3:+83339779 | Msa1131020:CDS |
| AGAACATAGCGAAGATGTGT+AGG | 0.601986 | 7_3:+83340052 | Msa1131020:CDS |
| AATGACCAAAGGCCAACTCA+AGG | 0.609242 | 7_3:+83339863 | Msa1131020:CDS |
| TTCAAGAATGCTCCAAAGGG+AGG | 0.614086 | 7_3:+83339943 | Msa1131020:CDS |
| ACTCAAGGATTTAATAGGGA+AGG | 0.618810 | 7_3:+83339878 | Msa1131020:CDS |
| TCACGGCTTGATCCCGAATG+CGG | 0.664295 | 7_3:-83339827 | None:intergenic |
| CAAGGGAGTGGTGCACCCTG+TGG | 0.671108 | 7_3:+83339797 | Msa1131020:CDS |
| ATCAAGGTGAGAATGACCAA+AGG | 0.688055 | 7_3:+83339852 | Msa1131020:CDS |
| GAATGCGGTGAACTACCACA+GGG | 0.775194 | 7_3:-83339812 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACAATGACAATTTAGAATT+AGG | + | chr7_3:83339909-83339928 | Msa1131020:CDS | 20.0% |
| ! | TCATACGTACAAGTTTTCAA+AGG | + | chr7_3:83340007-83340026 | Msa1131020:CDS | 30.0% |
| ! | CATACGTACAAGTTTTCAAA+GGG | + | chr7_3:83340008-83340027 | Msa1131020:CDS | 30.0% |
| ! | ATACGTACAAGTTTTCAAAG+GGG | + | chr7_3:83340009-83340028 | Msa1131020:CDS | 30.0% |
| GCCAACTCAAGGATTTAATA+GGG | + | chr7_3:83339874-83339893 | Msa1131020:CDS | 35.0% | |
| TCCCTATTAAATCCTTGAGT+TGG | - | chr7_3:83339878-83339897 | None:intergenic | 35.0% | |
| ACTCAAGGATTTAATAGGGA+AGG | + | chr7_3:83339878-83339897 | Msa1131020:CDS | 35.0% | |
| AGCATTCTTGAACGATCAAA+TGG | - | chr7_3:83339935-83339954 | None:intergenic | 35.0% | |
| TCTTCGCTATGTTCTTGAAT+TGG | - | chr7_3:83340047-83340066 | None:intergenic | 35.0% | |
| TGGAAAGAAGATGAGCAACA+AGG | + | chr7_3:83339779-83339798 | Msa1131020:CDS | 40.0% | |
| GGAAAGAAGATGAGCAACAA+GGG | + | chr7_3:83339780-83339799 | Msa1131020:CDS | 40.0% | |
| ATCAAGGTGAGAATGACCAA+AGG | + | chr7_3:83339852-83339871 | Msa1131020:CDS | 40.0% | |
| !! | TAAATCCTTGAGTTGGCCTT+TGG | - | chr7_3:83339871-83339890 | None:intergenic | 40.0% |
| GGCCAACTCAAGGATTTAAT+AGG | + | chr7_3:83339873-83339892 | Msa1131020:CDS | 40.0% | |
| CAAGGATTTAATAGGGAAGG+TGG | + | chr7_3:83339881-83339900 | Msa1131020:CDS | 40.0% | |
| ATCGTTCAAGAATGCTCCAA+AGG | + | chr7_3:83339939-83339958 | Msa1131020:CDS | 40.0% | |
| TCGTTCAAGAATGCTCCAAA+GGG | + | chr7_3:83339940-83339959 | Msa1131020:CDS | 40.0% | |
| TTTCAAAGGGGCTGAGTTTA+AGG | + | chr7_3:83340021-83340040 | Msa1131020:CDS | 40.0% | |
| CATTCTCACCTTGATCGTCA+CGG | - | chr7_3:83339847-83339866 | None:intergenic | 45.0% | |
| AATGACCAAAGGCCAACTCA+AGG | + | chr7_3:83339863-83339882 | Msa1131020:CDS | 45.0% | |
| TTCAAGAATGCTCCAAAGGG+AGG | + | chr7_3:83339943-83339962 | Msa1131020:CDS | 45.0% | |
| GAAGATGAGCAACAAGGGAG+TGG | + | chr7_3:83339785-83339804 | Msa1131020:CDS | 50.0% | |
| GAATGCGGTGAACTACCACA+GGG | - | chr7_3:83339815-83339834 | None:intergenic | 50.0% | |
| CTGTGGTAGTTCACCGCATT+CGG | + | chr7_3:83339814-83339833 | Msa1131020:CDS | 50.0% | |
| TGTGGTAGTTCACCGCATTC+GGG | + | chr7_3:83339815-83339834 | Msa1131020:CDS | 50.0% | |
| CGTGTTGTAACTGCTGCAGT+TGG | + | chr7_3:83339975-83339994 | Msa1131020:CDS | 50.0% | |
| CGAATGCGGTGAACTACCAC+AGG | - | chr7_3:83339816-83339835 | None:intergenic | 55.0% | |
| TCACGGCTTGATCCCGAATG+CGG | - | chr7_3:83339830-83339849 | None:intergenic | 55.0% | |
| GGATCAAGCCGTGACGATCA+AGG | + | chr7_3:83339836-83339855 | Msa1131020:CDS | 55.0% | |
| ACGAGCACGAAGCCTCCCTT+TGG | - | chr7_3:83339958-83339977 | None:intergenic | 60.0% | |
| CAAGGGAGTGGTGCACCCTG+TGG | + | chr7_3:83339797-83339816 | Msa1131020:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 83339762 | 83340073 | 83339762 | ID=Msa1131020;Name=Msa1131020 |
| chr7_3 | mRNA | 83339762 | 83340073 | 83339762 | ID=Msa1131020-mRNA-1;Parent=Msa1131020;Name=Msa1131020-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|103 |
| chr7_3 | exon | 83339762 | 83340073 | 83339762 | ID=Msa1131020-mRNA-1:exon:24754;Parent=Msa1131020-mRNA-1 |
| chr7_3 | CDS | 83339762 | 83340073 | 83339762 | ID=Msa1131020-mRNA-1:cds;Parent=Msa1131020-mRNA-1 |
| Gene Sequence |
| Protein sequence |
