Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1425060 | AES82811.1 | 95.192 | 104 | 4 | 1 | 1 | 103 | 1 | 104 | 1.60e-64 | 200 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1425060 | G7L533 | 95.192 | 104 | 4 | 1 | 1 | 103 | 1 | 104 | 7.63e-65 | 200 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017120 | Msa1425060 | 0.818544 | 1.854329e-52 | -8.615850e-47 |
| Msa0065390 | Msa1425060 | 0.802900 | 4.505377e-49 | -8.615850e-47 |
| Msa0110710 | Msa1425060 | 0.806836 | 6.778719e-50 | -8.615850e-47 |
| Msa1350670 | Msa1425060 | 0.806620 | 7.530167e-50 | -8.615850e-47 |
| Msa1355400 | Msa1425060 | 0.806806 | 6.878453e-50 | -8.615850e-47 |
| Msa1365020 | Msa1425060 | 0.813045 | 3.122780e-51 | -8.615850e-47 |
| Msa1367410 | Msa1425060 | 0.834581 | 2.759379e-56 | -8.615850e-47 |
| Msa1405770 | Msa1425060 | 0.801303 | 9.595108e-49 | -8.615850e-47 |
| Msa1417650 | Msa1425060 | 0.813044 | 3.123141e-51 | -8.615850e-47 |
| Msa1420060 | Msa1425060 | 0.815378 | 9.535179e-52 | -8.615850e-47 |
| Msa1425060 | Msa1434020 | 0.804109 | 2.529269e-49 | -8.615850e-47 |
| Msa1425060 | Msa1439670 | 0.803254 | 3.806509e-49 | -8.615850e-47 |
| Msa1425060 | Msa1444450 | 0.812860 | 3.427663e-51 | -8.615850e-47 |
| Msa0614230 | Msa1425060 | 0.806305 | 8.775504e-50 | -8.615850e-47 |
| Msa0621770 | Msa1425060 | 0.803620 | 3.196122e-49 | -8.615850e-47 |
| Msa0630970 | Msa1425060 | 0.809080 | 2.258221e-50 | -8.615850e-47 |
| Msa0640880 | Msa1425060 | 0.800576 | 1.350796e-48 | -8.615850e-47 |
| Msa0642910 | Msa1425060 | 0.839910 | 1.194293e-57 | -8.615850e-47 |
| Msa0663090 | Msa1425060 | 0.802425 | 5.645404e-49 | -8.615850e-47 |
| Msa0678890 | Msa1425060 | 0.837175 | 6.069115e-57 | -8.615850e-47 |
| Msa0690560 | Msa1425060 | 0.814932 | 1.197692e-51 | -8.615850e-47 |
| Msa0720270 | Msa1425060 | 0.813886 | 2.039203e-51 | -8.615850e-47 |
| Msa0722940 | Msa1425060 | 0.806204 | 9.213063e-50 | -8.615850e-47 |
| Msa0723150 | Msa1425060 | 0.814152 | 1.781877e-51 | -8.615850e-47 |
| Msa0782330 | Msa1425060 | 0.808805 | 2.585287e-50 | -8.615850e-47 |
| Msa0803130 | Msa1425060 | 0.832294 | 1.025967e-55 | -8.615850e-47 |
| Msa0810020 | Msa1425060 | 0.802089 | 6.619212e-49 | -8.615850e-47 |
| Msa0841490 | Msa1425060 | 0.835801 | 1.358406e-56 | -8.615850e-47 |
| Msa0141670 | Msa1425060 | 0.812967 | 3.247172e-51 | -8.615850e-47 |
| Msa0157020 | Msa1425060 | 0.833978 | 3.908492e-56 | -8.615850e-47 |
| Msa0207180 | Msa1425060 | 0.834108 | 3.626690e-56 | -8.615850e-47 |
| Msa1254190 | Msa1425060 | 0.803174 | 3.952695e-49 | -8.615850e-47 |
| Msa1308950 | Msa1425060 | 0.805932 | 1.051112e-49 | -8.615850e-47 |
| Msa0370600 | Msa1425060 | 0.804393 | 2.206828e-49 | -8.615850e-47 |
| Msa0380060 | Msa1425060 | 0.803251 | 3.810861e-49 | -8.615850e-47 |
| Msa0408640 | Msa1425060 | 0.831293 | 1.812332e-55 | -8.615850e-47 |
| Msa0418200 | Msa1425060 | 0.809792 | 1.588118e-50 | -8.615850e-47 |
| Msa0467800 | Msa1425060 | 0.800143 | 1.655213e-48 | -8.615850e-47 |
| Msa1106810 | Msa1425060 | 0.804915 | 1.717717e-49 | -8.615850e-47 |
| Msa0274780 | Msa1425060 | 0.810147 | 1.331912e-50 | -8.615850e-47 |
| Msa0277530 | Msa1425060 | 0.813898 | 2.027288e-51 | -8.615850e-47 |
| Msa0291040 | Msa1425060 | 0.802867 | 4.576287e-49 | -8.615850e-47 |
| Msa0297920 | Msa1425060 | 0.807035 | 6.152640e-50 | -8.615850e-47 |
| Msa0315480 | Msa1425060 | 0.831835 | 1.332344e-55 | -8.615850e-47 |
| Msa0361250 | Msa1425060 | 0.804176 | 2.449293e-49 | -8.615850e-47 |
| Msa0361260 | Msa1425060 | 0.830013 | 3.730415e-55 | -8.615850e-47 |
| Msa1130690 | Msa1425060 | 0.980626 | 1.176333e-150 | -8.615850e-47 |
| Msa1131020 | Msa1425060 | 0.975613 | 2.830605e-140 | -8.615850e-47 |
| Msa1200920 | Msa1425060 | 0.803089 | 4.116776e-49 | -8.615850e-47 |
| Msa0519200 | Msa1425060 | 0.811708 | 6.114626e-51 | -8.615850e-47 |
| Msa0524210 | Msa1425060 | 0.828691 | 7.809015e-55 | -8.615850e-47 |
| Msa0538570 | Msa1425060 | 0.811093 | 8.317478e-51 | -8.615850e-47 |
| Msa0547050 | Msa1425060 | 0.844235 | 8.571333e-59 | -8.615850e-47 |
| Msa0594390 | Msa1425060 | 0.811856 | 5.679719e-51 | -8.615850e-47 |
| Msa0599690 | Msa1425060 | 0.809169 | 2.160547e-50 | -8.615850e-47 |
| Msa0600390 | Msa1425060 | 0.808842 | 2.538961e-50 | -8.615850e-47 |
| Msa0601780 | Msa1425060 | 0.822679 | 2.082175e-53 | -8.615850e-47 |
| Msa0877420 | Msa1425060 | 0.821487 | 3.934741e-53 | -8.615850e-47 |
| Msa0905550 | Msa1425060 | 0.803433 | 3.495048e-49 | -8.615850e-47 |
| Msa0931480 | Msa1425060 | 0.806060 | 9.880800e-50 | -8.615850e-47 |
| Msa0931610 | Msa1425060 | 0.805465 | 1.317745e-49 | -8.615850e-47 |
| Msa0937550 | Msa1425060 | 0.824482 | 7.881519e-54 | -8.615850e-47 |
| Msa0942730 | Msa1425060 | 0.811945 | 5.430876e-51 | -8.615850e-47 |
| Msa0958020 | Msa1425060 | 0.811422 | 7.056644e-51 | -8.615850e-47 |
| Msa0974750 | Msa1425060 | 0.815604 | 8.490202e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1425060 | MtrunA17_Chr7g0276141 | 95.192 | 104 | 4 | 1 | 1 | 103 | 1 | 104 | 1.47e-68 | 200 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCAAAGGGGCTGAGTTTA+AGG | 0.194747 | tig0025818:+34741 | Msa1425060:CDS |
| GGCCAACTCAAGGATTTAAT+AGG | 0.281462 | tig0025818:+34593 | Msa1425060:CDS |
| AACAATGACAATTTAGAATT+AGG | 0.285252 | tig0025818:+34629 | Msa1425060:CDS |
| CTGTGGTAGTTCACCGCATT+CGG | 0.308570 | tig0025818:+34534 | Msa1425060:CDS |
| TCTTCGCTATGTTCTTGAAT+TGG | 0.381899 | tig0025818:-34764 | None:intergenic |
| TAAATCCTTGAGTTGGCCTT+TGG | 0.387136 | tig0025818:-34588 | None:intergenic |
| AAAATGGGTAATGCTTGCTT+TGG | 0.394791 | tig0025818:+34479 | None:intergenic |
| GCCAACTCAAGGATTTAATA+GGG | 0.437318 | tig0025818:+34594 | Msa1425060:CDS |
| TGTGGTAGTTCACCGCATTC+GGG | 0.441159 | tig0025818:+34535 | Msa1425060:CDS |
| ACGAGCACGAAGCCTCCCTT+TGG | 0.474923 | tig0025818:-34675 | None:intergenic |
| TCCCTATTAAATCCTTGAGT+TGG | 0.476466 | tig0025818:-34595 | None:intergenic |
| AGCATTCTTGAACGATCAAA+TGG | 0.501702 | tig0025818:-34652 | None:intergenic |
| GGATCAAGCCGTGACGATCA+AGG | 0.532320 | tig0025818:+34556 | Msa1425060:CDS |
| CGAATGCGGTGAACTACCAC+AGG | 0.532604 | tig0025818:-34533 | None:intergenic |
| ATCGTTCAAGAATGCTCCAA+AGG | 0.558651 | tig0025818:+34659 | Msa1425060:CDS |
| CGTGTTGTAACTGCTGCAGT+TGG | 0.558826 | tig0025818:+34695 | Msa1425060:CDS |
| CAAGGATTTAATAGGGAAGG+TGG | 0.561353 | tig0025818:+34601 | Msa1425060:CDS |
| TCGTTCAAGAATGCTCCAAA+GGG | 0.578470 | tig0025818:+34660 | Msa1425060:CDS |
| CATTCTCACCTTGATCGTCA+CGG | 0.582038 | tig0025818:-34564 | None:intergenic |
| GAAGATGAGCAACAAGGGAG+TGG | 0.588540 | tig0025818:+34505 | Msa1425060:CDS |
| GGAAAGAAGATGAGCAACAA+GGG | 0.598826 | tig0025818:+34500 | Msa1425060:CDS |
| TGGAAAGAAGATGAGCAACA+AGG | 0.599291 | tig0025818:+34499 | Msa1425060:CDS |
| AGAACATAGCGAAGATGTGT+AGG | 0.601986 | tig0025818:+34772 | Msa1425060:CDS |
| AATGACCAAAGGCCAACTCA+AGG | 0.609242 | tig0025818:+34583 | Msa1425060:CDS |
| TTCAAGAATGCTCCAAAGGG+AGG | 0.614086 | tig0025818:+34663 | Msa1425060:CDS |
| ACTCAAGGATTTAATAGGGA+AGG | 0.618810 | tig0025818:+34598 | Msa1425060:CDS |
| TCACGGCTTGATCCCGAATG+CGG | 0.664295 | tig0025818:-34547 | None:intergenic |
| CAAGGGAGTGGTGCACCCTG+TGG | 0.671108 | tig0025818:+34517 | Msa1425060:CDS |
| ATCAAGGTGAGAATGACCAA+AGG | 0.688055 | tig0025818:+34572 | Msa1425060:CDS |
| GAATGCGGTGAACTACCACA+GGG | 0.775194 | tig0025818:-34532 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACAATGACAATTTAGAATT+AGG | + | tig0025818:34629-34648 | Msa1425060:CDS | 20.0% |
| ! | TCATACGTACAAGTTTTCAA+AGG | + | tig0025818:34727-34746 | Msa1425060:CDS | 30.0% |
| ! | CATACGTACAAGTTTTCAAA+GGG | + | tig0025818:34728-34747 | Msa1425060:CDS | 30.0% |
| ! | ATACGTACAAGTTTTCAAAG+GGG | + | tig0025818:34729-34748 | Msa1425060:CDS | 30.0% |
| GCCAACTCAAGGATTTAATA+GGG | + | tig0025818:34594-34613 | Msa1425060:CDS | 35.0% | |
| TCCCTATTAAATCCTTGAGT+TGG | - | tig0025818:34598-34617 | None:intergenic | 35.0% | |
| ACTCAAGGATTTAATAGGGA+AGG | + | tig0025818:34598-34617 | Msa1425060:CDS | 35.0% | |
| AGCATTCTTGAACGATCAAA+TGG | - | tig0025818:34655-34674 | None:intergenic | 35.0% | |
| TCTTCGCTATGTTCTTGAAT+TGG | - | tig0025818:34767-34786 | None:intergenic | 35.0% | |
| TGGAAAGAAGATGAGCAACA+AGG | + | tig0025818:34499-34518 | Msa1425060:CDS | 40.0% | |
| GGAAAGAAGATGAGCAACAA+GGG | + | tig0025818:34500-34519 | Msa1425060:CDS | 40.0% | |
| ATCAAGGTGAGAATGACCAA+AGG | + | tig0025818:34572-34591 | Msa1425060:CDS | 40.0% | |
| !! | TAAATCCTTGAGTTGGCCTT+TGG | - | tig0025818:34591-34610 | None:intergenic | 40.0% |
| GGCCAACTCAAGGATTTAAT+AGG | + | tig0025818:34593-34612 | Msa1425060:CDS | 40.0% | |
| CAAGGATTTAATAGGGAAGG+TGG | + | tig0025818:34601-34620 | Msa1425060:CDS | 40.0% | |
| ATCGTTCAAGAATGCTCCAA+AGG | + | tig0025818:34659-34678 | Msa1425060:CDS | 40.0% | |
| TCGTTCAAGAATGCTCCAAA+GGG | + | tig0025818:34660-34679 | Msa1425060:CDS | 40.0% | |
| TTTCAAAGGGGCTGAGTTTA+AGG | + | tig0025818:34741-34760 | Msa1425060:CDS | 40.0% | |
| CATTCTCACCTTGATCGTCA+CGG | - | tig0025818:34567-34586 | None:intergenic | 45.0% | |
| AATGACCAAAGGCCAACTCA+AGG | + | tig0025818:34583-34602 | Msa1425060:CDS | 45.0% | |
| TTCAAGAATGCTCCAAAGGG+AGG | + | tig0025818:34663-34682 | Msa1425060:CDS | 45.0% | |
| GAAGATGAGCAACAAGGGAG+TGG | + | tig0025818:34505-34524 | Msa1425060:CDS | 50.0% | |
| GAATGCGGTGAACTACCACA+GGG | - | tig0025818:34535-34554 | None:intergenic | 50.0% | |
| CTGTGGTAGTTCACCGCATT+CGG | + | tig0025818:34534-34553 | Msa1425060:CDS | 50.0% | |
| TGTGGTAGTTCACCGCATTC+GGG | + | tig0025818:34535-34554 | Msa1425060:CDS | 50.0% | |
| CGTGTTGTAACTGCTGCAGT+TGG | + | tig0025818:34695-34714 | Msa1425060:CDS | 50.0% | |
| CGAATGCGGTGAACTACCAC+AGG | - | tig0025818:34536-34555 | None:intergenic | 55.0% | |
| TCACGGCTTGATCCCGAATG+CGG | - | tig0025818:34550-34569 | None:intergenic | 55.0% | |
| GGATCAAGCCGTGACGATCA+AGG | + | tig0025818:34556-34575 | Msa1425060:CDS | 55.0% | |
| ACGAGCACGAAGCCTCCCTT+TGG | - | tig0025818:34678-34697 | None:intergenic | 60.0% | |
| CAAGGGAGTGGTGCACCCTG+TGG | + | tig0025818:34517-34536 | Msa1425060:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0025818 | gene | 34482 | 34793 | 34482 | ID=Msa1425060;Name=Msa1425060 |
| tig0025818 | mRNA | 34482 | 34793 | 34482 | ID=Msa1425060-mRNA-1;Parent=Msa1425060;Name=Msa1425060-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|103 |
| tig0025818 | exon | 34482 | 34793 | 34482 | ID=Msa1425060-mRNA-1:exon:4115;Parent=Msa1425060-mRNA-1 |
| tig0025818 | CDS | 34482 | 34793 | 34482 | ID=Msa1425060-mRNA-1:cds;Parent=Msa1425060-mRNA-1 |
| Gene Sequence |
| Protein sequence |
