AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0630970


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0630970 MtrunA17_Chr4g0008421 100.000 85 0 0 2 86 205 289 3.14e-56 174
Msa0630970 MtrunA17_Chr4g0008411 81.395 86 15 1 1 85 115 200 1.33e-42 139
Msa0630970 MtrunA17_Chr7g0266341 40.741 81 45 2 9 86 212 292 1.11e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0630970 AT2G37970.1 76.744 86 20 0 1 86 161 246 1.14e-44 144

Find 23 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGGAAGGTGAGAGGAAATA+TGG 0.245932 4_3:+13482074 Msa0630970:CDS
GGAGAAGTTGAGGCTGAGTT+TGG 0.256967 4_3:+13482148 Msa0630970:CDS
ACACATCATGTTCATTCAAT+AGG 0.342036 4_3:-13482266 None:intergenic
CTTGGACACTTCCAATGTTT+AGG 0.347372 4_3:+13482225 Msa0630970:CDS
CACATCATGTTCATTCAATA+GGG 0.408624 4_3:-13482265 None:intergenic
TATGGAGTGGTGAAGTTTAG+TGG 0.463832 4_3:+13482092 Msa0630970:CDS
AGAGTTGTGATAAGAGAGGA+AGG 0.470240 4_3:+13482059 Msa0630970:CDS
AGGCTGAGTTTGGAGAGAGA+TGG 0.496209 4_3:+13482158 Msa0630970:CDS
AGGAGAAGGTGGAGAAGTTG+AGG 0.502041 4_3:+13482138 Msa0630970:CDS
ACTCTCTCATCAGTAGGCTT+AGG 0.503647 4_3:-13482041 None:intergenic
TCCAATGTTTAGGACTAATG+AGG 0.518493 4_3:+13482235 Msa0630970:CDS
TGATAAGAGAGGAAGGTGAG+AGG 0.519680 4_3:+13482066 Msa0630970:CDS
TGAGAGAGTTGTGATAAGAG+AGG 0.535873 4_3:+13482055 Msa0630970:CDS
AGCTAGTGATGAAGTGGTGA+AGG 0.543102 4_3:+13482118 Msa0630970:CDS
TGAAGTGGTGAAGGAGAAGG+TGG 0.549018 4_3:+13482127 Msa0630970:CDS
CTAAACATTGGAAGTGTCCA+AGG 0.551702 4_3:-13482224 None:intergenic
TGATGAAGTGGTGAAGGAGA+AGG 0.557865 4_3:+13482124 Msa0630970:CDS
ACCTCATTAGTCCTAAACAT+TGG 0.572004 4_3:-13482236 None:intergenic
AGGTGAGAGGAAATATGGAG+TGG 0.613327 4_3:+13482079 Msa0630970:CDS
ATCACAACTCTCTCATCAGT+AGG 0.625874 4_3:-13482047 None:intergenic
TGGGTAGGTACAATCCACCT+TGG 0.629812 4_3:+13482207 Msa0630970:CDS
TGGTGTAGCTAGTGATGAAG+TGG 0.645460 4_3:+13482112 Msa0630970:CDS
AACATTGGAAGTGTCCAAGG+TGG 0.685808 4_3:-13482221 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAGATGGTTTTAAGGTTAT+TGG + chr4_3:13482173-13482192 Msa0630970:CDS 30.0%
!!! GAGATGGTTTTAAGGTTATT+GGG + chr4_3:13482174-13482193 Msa0630970:CDS 30.0%
!!! AGATGGTTTTAAGGTTATTG+GGG + chr4_3:13482175-13482194 Msa0630970:CDS 30.0%
!!! GTTATTGGGGATTTTTTGTT+GGG + chr4_3:13482188-13482207 Msa0630970:CDS 30.0%
!! TTTGGAGAGAGATGGTTTTA+AGG + chr4_3:13482166-13482185 Msa0630970:CDS 35.0%
!!! GGTTATTGGGGATTTTTTGT+TGG + chr4_3:13482187-13482206 Msa0630970:CDS 35.0%
! TCCAATGTTTAGGACTAATG+AGG + chr4_3:13482235-13482254 Msa0630970:CDS 35.0%
ACCTCATTAGTCCTAAACAT+TGG - chr4_3:13482239-13482258 None:intergenic 35.0%
ATCACAACTCTCTCATCAGT+AGG - chr4_3:13482050-13482069 None:intergenic 40.0%
TGAGAGAGTTGTGATAAGAG+AGG + chr4_3:13482055-13482074 Msa0630970:CDS 40.0%
AGAGTTGTGATAAGAGAGGA+AGG + chr4_3:13482059-13482078 Msa0630970:CDS 40.0%
TATGGAGTGGTGAAGTTTAG+TGG + chr4_3:13482092-13482111 Msa0630970:CDS 40.0%
!!! TTGGGGATTTTTTGTTGGGT+AGG + chr4_3:13482192-13482211 Msa0630970:CDS 40.0%
!! CTAAACATTGGAAGTGTCCA+AGG - chr4_3:13482227-13482246 None:intergenic 40.0%
CTTGGACACTTCCAATGTTT+AGG + chr4_3:13482225-13482244 Msa0630970:CDS 40.0%
ACTCTCTCATCAGTAGGCTT+AGG - chr4_3:13482044-13482063 None:intergenic 45.0%
TGATAAGAGAGGAAGGTGAG+AGG + chr4_3:13482066-13482085 Msa0630970:CDS 45.0%
GAGGAAGGTGAGAGGAAATA+TGG + chr4_3:13482074-13482093 Msa0630970:CDS 45.0%
AGGTGAGAGGAAATATGGAG+TGG + chr4_3:13482079-13482098 Msa0630970:CDS 45.0%
TGGTGTAGCTAGTGATGAAG+TGG + chr4_3:13482112-13482131 Msa0630970:CDS 45.0%
AGCTAGTGATGAAGTGGTGA+AGG + chr4_3:13482118-13482137 Msa0630970:CDS 45.0%
TGATGAAGTGGTGAAGGAGA+AGG + chr4_3:13482124-13482143 Msa0630970:CDS 45.0%
!! AACATTGGAAGTGTCCAAGG+TGG - chr4_3:13482224-13482243 None:intergenic 45.0%
TGAAGTGGTGAAGGAGAAGG+TGG + chr4_3:13482127-13482146 Msa0630970:CDS 50.0%
AGGAGAAGGTGGAGAAGTTG+AGG + chr4_3:13482138-13482157 Msa0630970:CDS 50.0%
GGAGAAGTTGAGGCTGAGTT+TGG + chr4_3:13482148-13482167 Msa0630970:CDS 50.0%
AGGCTGAGTTTGGAGAGAGA+TGG + chr4_3:13482158-13482177 Msa0630970:CDS 50.0%
TGGGTAGGTACAATCCACCT+TGG + chr4_3:13482207-13482226 Msa0630970:CDS 50.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 13482017 13482277 13482017 ID=Msa0630970;Name=Msa0630970
chr4_3 mRNA 13482017 13482277 13482017 ID=Msa0630970-mRNA-1;Parent=Msa0630970;Name=Msa0630970-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|86
chr4_3 exon 13482017 13482277 13482017 ID=Msa0630970-mRNA-1:exon:3787;Parent=Msa0630970-mRNA-1
chr4_3 CDS 13482017 13482277 13482017 ID=Msa0630970-mRNA-1:cds;Parent=Msa0630970-mRNA-1
Gene Sequence

>Msa0630970

ATGTATGAAAAAGCAGAAGAAGCACCTAAGCCTACTGATGAGAGAGTTGTGATAAGAGAGGAAGGTGAGAGGAAATATGGAGTGGTGAAGTTTAGTGGTGTAGCTAGTGATGAAGTGGTGAAGGAGAAGGTGGAGAAGTTGAGGCTGAGTTTGGAGAGAGATGGTTTTAAGGTTATTGGGGATTTTTTGTTGGGTAGGTACAATCCACCTTGGACACTTCCAATGTTTAGGACTAATGAGGTTATGATCCCTATTGAATGA

Protein sequence

>Msa0630970

MYEKAEEAPKPTDERVVIREEGERKYGVVKFSGVASDEVVKEKVEKLRLSLERDGFKVIGDFLLGRYNPPWTLPMFRTNEVMIPIE*