AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0590660


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0590660 MtrunA17_Chr4g0008421 98.515 202 3 0 1 202 88 289 4.09e-145 405
Msa0590660 MtrunA17_Chr4g0008411 73.659 205 45 3 1 201 1 200 8.30e-103 296
Msa0590660 MtrunA17_Chr7g0266341 36.098 205 106 9 10 202 101 292 8.08e-25 98.6
Msa0590660 MtrunA17_Chr2g0306771 34.184 196 98 7 10 202 210 377 1.88e-19 85.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0590660 AT2G37970.1 69.907 216 50 2 1 202 32 246 3.12e-103 298
Msa0590660 AT3G10130.1 35.149 202 112 8 10 202 114 305 3.00e-19 84.0
Msa0590660 AT5G20140.1 32.143 196 103 6 10 202 205 373 4.07e-19 84.3
Msa0590660 AT5G20140.2 31.351 185 99 5 10 191 205 364 9.64e-18 80.9

Find 44 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACAAAGATGGTGGCTTTA+TGG 0.188087 4_2:+14220648 Msa0590660:CDS
TCCATAATGGGTATGGTATT+TGG 0.221891 4_2:+14220505 None:intergenic
GAGGAAGGTGAGAGGAAATA+TGG 0.245932 4_2:+14220916 Msa0590660:CDS
GGAGAAGTTGAGGCTGAGTT+TGG 0.256967 4_2:+14220990 Msa0590660:CDS
GCTAACTATATTGGAGCATT+AGG 0.273807 4_2:+14220676 Msa0590660:CDS
TCTATCATGGTTCATTCAAT+AGG 0.301833 4_2:-14221108 None:intergenic
CTTGGACACTTCCAATGTTT+AGG 0.339937 4_2:+14221067 Msa0590660:CDS
GCAGAACCCTTTGTGATAAC+TGG 0.387111 4_2:-14220742 None:intergenic
TTGTTTCCTTTGAACTGTGA+TGG 0.417876 4_2:-14220631 None:intergenic
TCACTAGCCTTAGTCACAAC+TGG 0.437262 4_2:-14220787 None:intergenic
TTTGAACTGTGATGGATCAT+AGG 0.439692 4_2:-14220623 None:intergenic
CTATCATGGTTCATTCAATA+GGG 0.452633 4_2:-14221107 None:intergenic
GACAGCACCAGTTGTGACTA+AGG 0.455361 4_2:+14220780 Msa0590660:CDS
AGGGTTGTGATAAGAGAGGA+AGG 0.486753 4_2:+14220901 Msa0590660:CDS
AGGCTGAGTTTGGAGAGAGA+TGG 0.496209 4_2:+14221000 Msa0590660:CDS
TTCTCGATCCATAATGGGTA+TGG 0.497976 4_2:+14220498 None:intergenic
AGGAGAAGGTGGAGAAGTTG+AGG 0.502041 4_2:+14220980 Msa0590660:CDS
TGAGAGGGTTGTGATAAGAG+AGG 0.509523 4_2:+14220897 Msa0590660:CDS
GTGACTAAGGCTAGTGAAGA+AGG 0.510099 4_2:+14220793 Msa0590660:CDS
TCCAATGTTTAGGACTAATG+AGG 0.518493 4_2:+14221077 Msa0590660:CDS
TGATAAGAGAGGAAGGTGAG+AGG 0.519680 4_2:+14220908 Msa0590660:CDS
TGGGTAGGTACAATCCTCCT+TGG 0.525821 4_2:+14221049 Msa0590660:CDS
ACCCTCTCATCAGTAGGCTT+AGG 0.529518 4_2:-14220883 None:intergenic
TGAAGTGGTGAAGGAGAAGG+TGG 0.549018 4_2:+14220969 Msa0590660:CDS
CTAAACATTGGAAGTGTCCA+AGG 0.554694 4_2:-14221066 None:intergenic
TCTGATTTCATAGTCTTGTG+TGG 0.560504 4_2:-14220569 None:intergenic
ACCTAAGCCTACTGATGAGA+GGG 0.571140 4_2:+14220882 Msa0590660:CDS
ACCTCATTAGTCCTAAACAT+TGG 0.572004 4_2:-14221078 None:intergenic
TGATGAAGTGGTGAAGGAGA+AGG 0.572950 4_2:+14220966 Msa0590660:CDS
ACTGCACCAGTTATCACAAA+GGG 0.579442 4_2:+14220736 Msa0590660:CDS
GGCAAGTGATGAAGTGGTGA+AGG 0.580010 4_2:+14220960 Msa0590660:CDS
TATGATCCATCACAGTTCAA+AGG 0.581780 4_2:+14220625 Msa0590660:CDS
CAGTTCAAAGGAAACAAAGA+TGG 0.590535 4_2:+14220637 Msa0590660:CDS
CACCTAAGCCTACTGATGAG+AGG 0.606748 4_2:+14220881 Msa0590660:CDS
AGAAGGTGAGAGCAACAAGA+TGG 0.609784 4_2:+14220810 Msa0590660:CDS
TATGGAGTGGTGAAGTTCAG+TGG 0.611850 4_2:+14220934 Msa0590660:CDS
GTGACTTCAGCTGCTACAGA+AGG 0.612236 4_2:-14220601 None:intergenic
AGGTGAGAGGAAATATGGAG+TGG 0.613327 4_2:+14220921 Msa0590660:CDS
TTCAAAGGAAACAAAGATGG+TGG 0.644336 4_2:+14220640 Msa0590660:CDS
GACTGCACCAGTTATCACAA+AGG 0.644397 4_2:+14220735 Msa0590660:CDS
TGGTGTGGCAAGTGATGAAG+TGG 0.645405 4_2:+14220954 Msa0590660:CDS
ATCACAACCCTCTCATCAGT+AGG 0.648561 4_2:-14220889 None:intergenic
AGTGGTGAAGTTCAGTGGTG+TGG 0.703765 4_2:+14220939 Msa0590660:CDS
AACATTGGAAGTGTCCAAGG+AGG 0.720771 4_2:-14221063 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTGTCACTTCATATTT+TGG - chr4_2:14220550-14220569 None:intergenic 25.0%
!!! ATGGTTTTAGCTAACTATAT+TGG + chr4_2:14220667-14220686 Msa0590660:CDS 25.0%
GGTATGGTATTTGGAAAAAT+TGG + chr4_2:14220514-14220533 Msa0590660:CDS 30.0%
!!! TCTGCTTTTTCATACATAGA+AGG - chr4_2:14220856-14220875 None:intergenic 30.0%
!!! AGAGATGGTTTTAAGGTTAT+TGG + chr4_2:14221015-14221034 Msa0590660:CDS 30.0%
!!! GAGATGGTTTTAAGGTTATT+GGG + chr4_2:14221016-14221035 Msa0590660:CDS 30.0%
!!! AGATGGTTTTAAGGTTATTG+GGG + chr4_2:14221017-14221036 Msa0590660:CDS 30.0%
!!! GTTATTGGGGATTTTTTGTT+GGG + chr4_2:14221030-14221049 Msa0590660:CDS 30.0%
!! TCTGATTTCATAGTCTTGTG+TGG - chr4_2:14220572-14220591 None:intergenic 35.0%
!! TTTGAACTGTGATGGATCAT+AGG - chr4_2:14220626-14220645 None:intergenic 35.0%
TATGATCCATCACAGTTCAA+AGG + chr4_2:14220625-14220644 Msa0590660:CDS 35.0%
TTGTTTCCTTTGAACTGTGA+TGG - chr4_2:14220634-14220653 None:intergenic 35.0%
CAGTTCAAAGGAAACAAAGA+TGG + chr4_2:14220637-14220656 Msa0590660:CDS 35.0%
TTCAAAGGAAACAAAGATGG+TGG + chr4_2:14220640-14220659 Msa0590660:CDS 35.0%
! AAACAAAGATGGTGGCTTTA+TGG + chr4_2:14220648-14220667 Msa0590660:CDS 35.0%
! GCTAACTATATTGGAGCATT+AGG + chr4_2:14220676-14220695 Msa0590660:CDS 35.0%
!!! TTTCAGGTTTTGTGTTCTGA+GGG - chr4_2:14220705-14220724 None:intergenic 35.0%
!!! TTTTCAGGTTTTGTGTTCTG+AGG - chr4_2:14220706-14220725 None:intergenic 35.0%
!! TTTGGAGAGAGATGGTTTTA+AGG + chr4_2:14221008-14221027 Msa0590660:CDS 35.0%
!!! GGTTATTGGGGATTTTTTGT+TGG + chr4_2:14221029-14221048 Msa0590660:CDS 35.0%
! TCCAATGTTTAGGACTAATG+AGG + chr4_2:14221077-14221096 Msa0590660:CDS 35.0%
ACCTCATTAGTCCTAAACAT+TGG - chr4_2:14221081-14221100 None:intergenic 35.0%
! GCAGTCATAGCAATCTTTTC+AGG - chr4_2:14220721-14220740 None:intergenic 40.0%
ACTGCACCAGTTATCACAAA+GGG + chr4_2:14220736-14220755 Msa0590660:CDS 40.0%
!!! TTGGGGATTTTTTGTTGGGT+AGG + chr4_2:14221034-14221053 Msa0590660:CDS 40.0%
!! CTAAACATTGGAAGTGTCCA+AGG - chr4_2:14221069-14221088 None:intergenic 40.0%
CTTGGACACTTCCAATGTTT+AGG + chr4_2:14221067-14221086 Msa0590660:CDS 40.0%
GACTGCACCAGTTATCACAA+AGG + chr4_2:14220735-14220754 Msa0590660:CDS 45.0%
GCAGAACCCTTTGTGATAAC+TGG - chr4_2:14220745-14220764 None:intergenic 45.0%
!! TCACTAGCCTTAGTCACAAC+TGG - chr4_2:14220790-14220809 None:intergenic 45.0%
GTGACTAAGGCTAGTGAAGA+AGG + chr4_2:14220793-14220812 Msa0590660:CDS 45.0%
AGAAGGTGAGAGCAACAAGA+TGG + chr4_2:14220810-14220829 Msa0590660:CDS 45.0%
ACCTAAGCCTACTGATGAGA+GGG + chr4_2:14220882-14220901 Msa0590660:CDS 45.0%
ATCACAACCCTCTCATCAGT+AGG - chr4_2:14220892-14220911 None:intergenic 45.0%
TGAGAGGGTTGTGATAAGAG+AGG + chr4_2:14220897-14220916 Msa0590660:CDS 45.0%
AGGGTTGTGATAAGAGAGGA+AGG + chr4_2:14220901-14220920 Msa0590660:CDS 45.0%
TGATAAGAGAGGAAGGTGAG+AGG + chr4_2:14220908-14220927 Msa0590660:CDS 45.0%
GAGGAAGGTGAGAGGAAATA+TGG + chr4_2:14220916-14220935 Msa0590660:CDS 45.0%
AGGTGAGAGGAAATATGGAG+TGG + chr4_2:14220921-14220940 Msa0590660:CDS 45.0%
TATGGAGTGGTGAAGTTCAG+TGG + chr4_2:14220934-14220953 Msa0590660:CDS 45.0%
TGATGAAGTGGTGAAGGAGA+AGG + chr4_2:14220966-14220985 Msa0590660:CDS 45.0%
!! AACATTGGAAGTGTCCAAGG+AGG - chr4_2:14221066-14221085 None:intergenic 45.0%
GTGACTTCAGCTGCTACAGA+AGG - chr4_2:14220604-14220623 None:intergenic 50.0%
GACAGCACCAGTTGTGACTA+AGG + chr4_2:14220780-14220799 Msa0590660:CDS 50.0%
CACCTAAGCCTACTGATGAG+AGG + chr4_2:14220881-14220900 Msa0590660:CDS 50.0%
ACCCTCTCATCAGTAGGCTT+AGG - chr4_2:14220886-14220905 None:intergenic 50.0%
AGTGGTGAAGTTCAGTGGTG+TGG + chr4_2:14220939-14220958 Msa0590660:CDS 50.0%
TGGTGTGGCAAGTGATGAAG+TGG + chr4_2:14220954-14220973 Msa0590660:CDS 50.0%
GGCAAGTGATGAAGTGGTGA+AGG + chr4_2:14220960-14220979 Msa0590660:CDS 50.0%
TGAAGTGGTGAAGGAGAAGG+TGG + chr4_2:14220969-14220988 Msa0590660:CDS 50.0%
AGGAGAAGGTGGAGAAGTTG+AGG + chr4_2:14220980-14220999 Msa0590660:CDS 50.0%
GGAGAAGTTGAGGCTGAGTT+TGG + chr4_2:14220990-14221009 Msa0590660:CDS 50.0%
AGGCTGAGTTTGGAGAGAGA+TGG + chr4_2:14221000-14221019 Msa0590660:CDS 50.0%
TGGGTAGGTACAATCCTCCT+TGG + chr4_2:14221049-14221068 Msa0590660:CDS 50.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 14220511 14221119 14220511 ID=Msa0590660;Name=Msa0590660
chr4_2 mRNA 14220511 14221119 14220511 ID=Msa0590660-mRNA-1;Parent=Msa0590660;Name=Msa0590660-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|202
chr4_2 exon 14220511 14221119 14220511 ID=Msa0590660-mRNA-1:exon:4128;Parent=Msa0590660-mRNA-1
chr4_2 CDS 14220511 14221119 14220511 ID=Msa0590660-mRNA-1:cds;Parent=Msa0590660-mRNA-1
Gene Sequence

>Msa0590660

ATGGGTATGGTATTTGGAAAAATTGGTGTTGAGACACCAAAATATGAAGTGACAAAAACCACACAAGACTATGAAATCAGAATATATGCACCTTCTGTAGCAGCTGAAGTCACCTATGATCCATCACAGTTCAAAGGAAACAAAGATGGTGGCTTTATGGTTTTAGCTAACTATATTGGAGCATTAGGTAACCCTCAGAACACAAAACCTGAAAAGATTGCTATGACTGCACCAGTTATCACAAAGGGTTCTGCAGAGAAGATTGCTATGACAGCACCAGTTGTGACTAAGGCTAGTGAAGAAGGTGAGAGCAACAAGATGGTGACTATGCAGTTTATTTTGCCTTCTATGTATGAAAAAGCAGAAGAAGCACCTAAGCCTACTGATGAGAGGGTTGTGATAAGAGAGGAAGGTGAGAGGAAATATGGAGTGGTGAAGTTCAGTGGTGTGGCAAGTGATGAAGTGGTGAAGGAGAAGGTGGAGAAGTTGAGGCTGAGTTTGGAGAGAGATGGTTTTAAGGTTATTGGGGATTTTTTGTTGGGTAGGTACAATCCTCCTTGGACACTTCCAATGTTTAGGACTAATGAGGTTATGATCCCTATTGAATGA

Protein sequence

>Msa0590660

MGMVFGKIGVETPKYEVTKTTQDYEIRIYAPSVAAEVTYDPSQFKGNKDGGFMVLANYIGALGNPQNTKPEKIAMTAPVITKGSAEKIAMTAPVVTKASEEGESNKMVTMQFILPSMYEKAEEAPKPTDERVVIREEGERKYGVVKFSGVASDEVVKEKVEKLRLSLERDGFKVIGDFLLGRYNPPWTLPMFRTNEVMIPIE*