Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1130690 | AES82811.1 | 95.192 | 104 | 4 | 1 | 1 | 103 | 1 | 104 | 1.60e-64 | 200 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1130690 | G7L533 | 95.192 | 104 | 4 | 1 | 1 | 103 | 1 | 104 | 7.63e-65 | 200 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017120 | Msa1130690 | 0.819969 | 8.782596e-53 | -8.615850e-47 |
| Msa0065390 | Msa1130690 | 0.806423 | 8.287239e-50 | -8.615850e-47 |
| Msa0110710 | Msa1130690 | 0.809705 | 1.658208e-50 | -8.615850e-47 |
| Msa0614230 | Msa1130690 | 0.809513 | 1.823157e-50 | -8.615850e-47 |
| Msa0621770 | Msa1130690 | 0.809104 | 2.231641e-50 | -8.615850e-47 |
| Msa0630970 | Msa1130690 | 0.814033 | 1.892504e-51 | -8.615850e-47 |
| Msa0634270 | Msa1130690 | 0.809084 | 2.254045e-50 | -8.615850e-47 |
| Msa0640880 | Msa1130690 | 0.811334 | 7.373562e-51 | -8.615850e-47 |
| Msa0642910 | Msa1130690 | 0.833455 | 5.280530e-56 | -8.615850e-47 |
| Msa0663090 | Msa1130690 | 0.812972 | 3.239139e-51 | -8.615850e-47 |
| Msa0678890 | Msa1130690 | 0.836853 | 7.334320e-57 | -8.615850e-47 |
| Msa0690560 | Msa1130690 | 0.806898 | 6.576953e-50 | -8.615850e-47 |
| Msa0720270 | Msa1130690 | 0.817531 | 3.141747e-52 | -8.615850e-47 |
| Msa0722940 | Msa1130690 | 0.810008 | 1.427099e-50 | -8.615850e-47 |
| Msa0723150 | Msa1130690 | 0.817584 | 3.057312e-52 | -8.615850e-47 |
| Msa0782330 | Msa1130690 | 0.804393 | 2.206927e-49 | -8.615850e-47 |
| Msa0803130 | Msa1130690 | 0.832690 | 8.187713e-56 | -8.615850e-47 |
| Msa0810020 | Msa1130690 | 0.807811 | 4.212278e-50 | -8.615850e-47 |
| Msa0841490 | Msa1130690 | 0.836121 | 1.126602e-56 | -8.615850e-47 |
| Msa0141670 | Msa1130690 | 0.808817 | 2.569753e-50 | -8.615850e-47 |
| Msa0157020 | Msa1130690 | 0.834079 | 3.688206e-56 | -8.615850e-47 |
| Msa0200970 | Msa1130690 | 0.801686 | 8.008512e-49 | -8.615850e-47 |
| Msa0207180 | Msa1130690 | 0.831593 | 1.528938e-55 | -8.615850e-47 |
| Msa0230400 | Msa1130690 | 0.803647 | 3.155374e-49 | -8.615850e-47 |
| Msa0370600 | Msa1130690 | 0.817176 | 3.777447e-52 | -8.615850e-47 |
| Msa0378340 | Msa1130690 | 0.800374 | 1.484876e-48 | -8.615850e-47 |
| Msa0380060 | Msa1130690 | 0.815893 | 7.319091e-52 | -8.615850e-47 |
| Msa0408640 | Msa1130690 | 0.830520 | 2.804774e-55 | -8.615850e-47 |
| Msa0418200 | Msa1130690 | 0.823292 | 1.498112e-53 | -8.615850e-47 |
| Msa0464540 | Msa1130690 | 0.808544 | 2.939248e-50 | -8.615850e-47 |
| Msa1007350 | Msa1130690 | 0.802980 | 4.336083e-49 | -8.615850e-47 |
| Msa1014270 | Msa1130690 | 0.806938 | 6.450300e-50 | -8.615850e-47 |
| Msa1073350 | Msa1130690 | 0.800938 | 1.139475e-48 | -8.615850e-47 |
| Msa1106810 | Msa1130690 | 0.801762 | 7.727221e-49 | -8.615850e-47 |
| Msa0274780 | Msa1130690 | 0.814778 | 1.295746e-51 | -8.615850e-47 |
| Msa0277530 | Msa1130690 | 0.814679 | 1.362448e-51 | -8.615850e-47 |
| Msa0291040 | Msa1130690 | 0.805502 | 1.294064e-49 | -8.615850e-47 |
| Msa0297920 | Msa1130690 | 0.817103 | 3.922322e-52 | -8.615850e-47 |
| Msa0315480 | Msa1130690 | 0.832036 | 1.188676e-55 | -8.615850e-47 |
| Msa0361250 | Msa1130690 | 0.803476 | 3.423948e-49 | -8.615850e-47 |
| Msa0361260 | Msa1130690 | 0.828890 | 6.988876e-55 | -8.615850e-47 |
| Msa1130690 | Msa1131020 | 0.957027 | 7.284097e-115 | -8.615850e-47 |
| Msa1130690 | Msa1150970 | 0.802706 | 4.940699e-49 | -8.615850e-47 |
| Msa1130690 | Msa1200920 | 0.803724 | 3.040520e-49 | -8.615850e-47 |
| Msa1130690 | Msa1215210 | 0.802238 | 6.166775e-49 | -8.615850e-47 |
| Msa1130690 | Msa1254190 | 0.806882 | 6.628020e-50 | -8.615850e-47 |
| Msa1130690 | Msa1308950 | 0.811264 | 7.635983e-51 | -8.615850e-47 |
| Msa1130690 | Msa1355400 | 0.819966 | 8.796228e-53 | -8.615850e-47 |
| Msa1130690 | Msa1365020 | 0.807022 | 6.190410e-50 | -8.615850e-47 |
| Msa1130690 | Msa1367410 | 0.819782 | 9.693630e-53 | -8.615850e-47 |
| Msa1130690 | Msa1420060 | 0.819513 | 1.116692e-52 | -8.615850e-47 |
| Msa1130690 | Msa1425060 | 0.980626 | 1.176333e-150 | -8.615850e-47 |
| Msa1130690 | Msa1434020 | 0.800980 | 1.117079e-48 | -8.615850e-47 |
| Msa1130690 | Msa1435050 | 0.802064 | 6.696760e-49 | -8.615850e-47 |
| Msa1130690 | Msa1439670 | 0.809154 | 2.176980e-50 | -8.615850e-47 |
| Msa1130690 | Msa1444450 | 0.813099 | 3.037554e-51 | -8.615850e-47 |
| Msa0519200 | Msa1130690 | 0.819280 | 1.261955e-52 | -8.615850e-47 |
| Msa0524210 | Msa1130690 | 0.811737 | 6.028984e-51 | -8.615850e-47 |
| Msa0538570 | Msa1130690 | 0.821780 | 3.365220e-53 | -8.615850e-47 |
| Msa0547050 | Msa1130690 | 0.846140 | 2.619180e-59 | -8.615850e-47 |
| Msa0594390 | Msa1130690 | 0.827547 | 1.473121e-54 | -8.615850e-47 |
| Msa0599690 | Msa1130690 | 0.811809 | 5.814913e-51 | -8.615850e-47 |
| Msa0600390 | Msa1130690 | 0.815804 | 7.661821e-52 | -8.615850e-47 |
| Msa0601780 | Msa1130690 | 0.826694 | 2.356955e-54 | -8.615850e-47 |
| Msa0873190 | Msa1130690 | 0.804244 | 2.371110e-49 | -8.615850e-47 |
| Msa0877420 | Msa1130690 | 0.817272 | 3.593818e-52 | -8.615850e-47 |
| Msa0905550 | Msa1130690 | 0.808205 | 3.472512e-50 | -8.615850e-47 |
| Msa0930330 | Msa1130690 | 0.805400 | 1.359510e-49 | -8.615850e-47 |
| Msa0931480 | Msa1130690 | 0.816177 | 6.326208e-52 | -8.615850e-47 |
| Msa0931610 | Msa1130690 | 0.813416 | 2.588177e-51 | -8.615850e-47 |
| Msa0937550 | Msa1130690 | 0.827379 | 1.616477e-54 | -8.615850e-47 |
| Msa0942730 | Msa1130690 | 0.805277 | 1.442488e-49 | -8.615850e-47 |
| Msa0951460 | Msa1130690 | 0.805194 | 1.501745e-49 | -8.615850e-47 |
| Msa0958020 | Msa1130690 | 0.813447 | 2.548181e-51 | -8.615850e-47 |
| Msa0974750 | Msa1130690 | 0.817937 | 2.545353e-52 | -8.615850e-47 |
| Msa0978000 | Msa1130690 | 0.803645 | 3.157333e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1130690 | MtrunA17_Chr7g0276141 | 95.192 | 104 | 4 | 1 | 1 | 103 | 1 | 104 | 1.47e-68 | 200 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCAAAGGGGCTGAGTTTA+AGG | 0.194747 | 7_3:-82874648 | Msa1130690:CDS |
| GGCCAACTCAAGGATTTAAT+AGG | 0.281462 | 7_3:-82874796 | Msa1130690:CDS |
| AACAATGACAATTTAGAATT+AGG | 0.285252 | 7_3:-82874760 | Msa1130690:CDS |
| CTGTGGTAGTTCACCGCATT+CGG | 0.308570 | 7_3:-82874855 | Msa1130690:CDS |
| TCTTCGCTATGTTCTTGAAT+TGG | 0.381899 | 7_3:+82874625 | None:intergenic |
| TAAATCCTTGAGTTGGCCTT+TGG | 0.387136 | 7_3:+82874801 | None:intergenic |
| AAAATGGGTAATGCTTGCTT+TGG | 0.394791 | 7_3:-82874910 | Msa1130690:CDS |
| GCCAACTCAAGGATTTAATA+GGG | 0.437318 | 7_3:-82874795 | Msa1130690:CDS |
| TGTGGTAGTTCACCGCATTC+GGG | 0.441159 | 7_3:-82874854 | Msa1130690:CDS |
| ACGAGCACGAAGCCTCCCTT+TGG | 0.474923 | 7_3:+82874714 | None:intergenic |
| TCCCTATTAAATCCTTGAGT+TGG | 0.476466 | 7_3:+82874794 | None:intergenic |
| AGCATTCTTGAACGATCAAA+TGG | 0.501702 | 7_3:+82874737 | None:intergenic |
| GGATCAAGCCGTGACGATCA+AGG | 0.532320 | 7_3:-82874833 | Msa1130690:CDS |
| CGAATGCGGTGAACTACCAC+AGG | 0.532604 | 7_3:+82874856 | None:intergenic |
| ATCGTTCAAGAATGCTCCAA+AGG | 0.558651 | 7_3:-82874730 | Msa1130690:CDS |
| CGTGTTGTAACTGCTGCAGT+TGG | 0.558826 | 7_3:-82874694 | Msa1130690:CDS |
| CAAGGATTTAATAGGGAAGG+TGG | 0.561353 | 7_3:-82874788 | Msa1130690:CDS |
| TCGTTCAAGAATGCTCCAAA+GGG | 0.578470 | 7_3:-82874729 | Msa1130690:CDS |
| CATTCTCACCTTGATCGTCA+CGG | 0.582038 | 7_3:+82874825 | None:intergenic |
| GAAGATGAGCAACAAGGGAG+TGG | 0.588540 | 7_3:-82874884 | Msa1130690:CDS |
| GGAAAGAAGATGAGCAACAA+GGG | 0.598826 | 7_3:-82874889 | Msa1130690:CDS |
| TGGAAAGAAGATGAGCAACA+AGG | 0.599291 | 7_3:-82874890 | Msa1130690:CDS |
| AGAACATAGCGAAGATGTGT+AGG | 0.601986 | 7_3:-82874617 | None:intergenic |
| AATGACCAAAGGCCAACTCA+AGG | 0.609242 | 7_3:-82874806 | Msa1130690:CDS |
| TTCAAGAATGCTCCAAAGGG+AGG | 0.614086 | 7_3:-82874726 | Msa1130690:CDS |
| ACTCAAGGATTTAATAGGGA+AGG | 0.618810 | 7_3:-82874791 | Msa1130690:CDS |
| TCACGGCTTGATCCCGAATG+CGG | 0.664295 | 7_3:+82874842 | None:intergenic |
| CAAGGGAGTGGTGCACCCTG+TGG | 0.671108 | 7_3:-82874872 | Msa1130690:CDS |
| ATCAAGGTGAGAATGACCAA+AGG | 0.688055 | 7_3:-82874817 | Msa1130690:CDS |
| GAATGCGGTGAACTACCACA+GGG | 0.775194 | 7_3:+82874857 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACAATGACAATTTAGAATT+AGG | - | chr7_3:82874765-82874784 | Msa1130690:CDS | 20.0% |
| ! | TCATACGTACAAGTTTTCAA+AGG | - | chr7_3:82874863-82874882 | Msa1130690:CDS | 30.0% |
| ! | CATACGTACAAGTTTTCAAA+GGG | - | chr7_3:82874864-82874883 | Msa1130690:CDS | 30.0% |
| ! | ATACGTACAAGTTTTCAAAG+GGG | - | chr7_3:82874865-82874884 | Msa1130690:CDS | 30.0% |
| GCCAACTCAAGGATTTAATA+GGG | - | chr7_3:82874730-82874749 | Msa1130690:CDS | 35.0% | |
| TCCCTATTAAATCCTTGAGT+TGG | + | chr7_3:82874734-82874753 | None:intergenic | 35.0% | |
| ACTCAAGGATTTAATAGGGA+AGG | - | chr7_3:82874734-82874753 | Msa1130690:CDS | 35.0% | |
| AGCATTCTTGAACGATCAAA+TGG | + | chr7_3:82874791-82874810 | None:intergenic | 35.0% | |
| TCTTCGCTATGTTCTTGAAT+TGG | + | chr7_3:82874903-82874922 | None:intergenic | 35.0% | |
| TGGAAAGAAGATGAGCAACA+AGG | - | chr7_3:82874635-82874654 | Msa1130690:CDS | 40.0% | |
| GGAAAGAAGATGAGCAACAA+GGG | - | chr7_3:82874636-82874655 | Msa1130690:CDS | 40.0% | |
| ATCAAGGTGAGAATGACCAA+AGG | - | chr7_3:82874708-82874727 | Msa1130690:CDS | 40.0% | |
| !! | TAAATCCTTGAGTTGGCCTT+TGG | + | chr7_3:82874727-82874746 | None:intergenic | 40.0% |
| GGCCAACTCAAGGATTTAAT+AGG | - | chr7_3:82874729-82874748 | Msa1130690:CDS | 40.0% | |
| CAAGGATTTAATAGGGAAGG+TGG | - | chr7_3:82874737-82874756 | Msa1130690:CDS | 40.0% | |
| ATCGTTCAAGAATGCTCCAA+AGG | - | chr7_3:82874795-82874814 | Msa1130690:CDS | 40.0% | |
| TCGTTCAAGAATGCTCCAAA+GGG | - | chr7_3:82874796-82874815 | Msa1130690:CDS | 40.0% | |
| TTTCAAAGGGGCTGAGTTTA+AGG | - | chr7_3:82874877-82874896 | Msa1130690:CDS | 40.0% | |
| CATTCTCACCTTGATCGTCA+CGG | + | chr7_3:82874703-82874722 | None:intergenic | 45.0% | |
| AATGACCAAAGGCCAACTCA+AGG | - | chr7_3:82874719-82874738 | Msa1130690:CDS | 45.0% | |
| TTCAAGAATGCTCCAAAGGG+AGG | - | chr7_3:82874799-82874818 | Msa1130690:CDS | 45.0% | |
| GAAGATGAGCAACAAGGGAG+TGG | - | chr7_3:82874641-82874660 | Msa1130690:CDS | 50.0% | |
| GAATGCGGTGAACTACCACA+GGG | + | chr7_3:82874671-82874690 | None:intergenic | 50.0% | |
| CTGTGGTAGTTCACCGCATT+CGG | - | chr7_3:82874670-82874689 | Msa1130690:CDS | 50.0% | |
| TGTGGTAGTTCACCGCATTC+GGG | - | chr7_3:82874671-82874690 | Msa1130690:CDS | 50.0% | |
| CGTGTTGTAACTGCTGCAGT+TGG | - | chr7_3:82874831-82874850 | Msa1130690:CDS | 50.0% | |
| CGAATGCGGTGAACTACCAC+AGG | + | chr7_3:82874672-82874691 | None:intergenic | 55.0% | |
| TCACGGCTTGATCCCGAATG+CGG | + | chr7_3:82874686-82874705 | None:intergenic | 55.0% | |
| GGATCAAGCCGTGACGATCA+AGG | - | chr7_3:82874692-82874711 | Msa1130690:CDS | 55.0% | |
| ACGAGCACGAAGCCTCCCTT+TGG | + | chr7_3:82874814-82874833 | None:intergenic | 60.0% | |
| CAAGGGAGTGGTGCACCCTG+TGG | - | chr7_3:82874653-82874672 | Msa1130690:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 82874618 | 82874929 | 82874618 | ID=Msa1130690;Name=Msa1130690 |
| chr7_3 | mRNA | 82874618 | 82874929 | 82874618 | ID=Msa1130690-mRNA-1;Parent=Msa1130690;Name=Msa1130690-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|103 |
| chr7_3 | exon | 82874618 | 82874929 | 82874618 | ID=Msa1130690-mRNA-1:exon:24545;Parent=Msa1130690-mRNA-1 |
| chr7_3 | CDS | 82874618 | 82874929 | 82874618 | ID=Msa1130690-mRNA-1:cds;Parent=Msa1130690-mRNA-1 |
| Gene Sequence |
| Protein sequence |
