AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1307150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1307150 MtrunA17_Chr8g0346321 90.299 134 13 0 1 134 116 249 1.55e-73 219
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATGGTTAATGTTTAATT+TGG 0.183753 8_4:+2480472 Msa1307150:three_prime_UTR
AATCACATGGTTCAATTCTT+TGG 0.203507 8_4:-2480371 None:intergenic
ACTTGACTCAACCAAGAGTT+TGG 0.334804 8_4:-2478391 None:intergenic
ATGGGAGTGTCACTGTTATC+TGG 0.356185 8_4:+2480283 Msa1307150:three_prime_UTR
GAAGATTATTCCCCTTGTTA+AGG 0.356621 8_4:+2478458 Msa1307150:CDS
GTTATCTGTGACTATATATA+TGG 0.364535 8_4:+2480456 Msa1307150:three_prime_UTR
AAAGAGGAAGAAATGATGAT+TGG 0.430176 8_4:+2478216 Msa1307150:exon
TGTGTTCATGTTGTTTCTGT+TGG 0.432041 8_4:+2480404 Msa1307150:three_prime_UTR
ACTTTCTTGATGTCTTTCAT+TGG 0.437252 8_4:-2478492 None:intergenic
ATGATTTCATGACAAATCAT+AGG 0.438088 8_4:-2478267 None:intergenic
AATGATGATTGGAAACTTCA+AGG 0.448863 8_4:+2478227 Msa1307150:exon
TGATTCTTGACATCAAGATC+TGG 0.455218 8_4:-2478858 None:intergenic
ATTTGATGAGAACAACTTTG+AGG 0.478699 8_4:+2478149 Msa1307150:five_prime_UTR
ATTAATAACCACATGAATGA+AGG 0.483710 8_4:+2478900 Msa1307150:CDS
ACGTATGAGATTAAATACTT+AGG 0.485233 8_4:+2480327 Msa1307150:three_prime_UTR
TGATGAAGAAGATGCTAAAG+AGG 0.486614 8_4:+2478826 Msa1307150:CDS
ACCACATGAATGAAGGCAAA+TGG 0.487764 8_4:+2478907 Msa1307150:CDS
TTATTCTGCAGCCAAACTCT+TGG 0.488552 8_4:+2478380 Msa1307150:CDS
TATGATTTGTCATGAAATCA+TGG 0.489589 8_4:+2478269 Msa1307150:CDS
TTGAAGCTGATCAAAAGAAA+AGG 0.490869 8_4:+2478304 Msa1307150:CDS
TGATGATGATGCTAGAAAAG+AGG 0.505541 8_4:+2478200 Msa1307150:five_prime_UTR
GCCATTTGCCTTCATTCATG+TGG 0.514475 8_4:-2478908 None:intergenic
TACTAGCTAGTGTTAAGCCA+TGG 0.527892 8_4:+2480264 Msa1307150:exon
AAACTTCAAGGAAATTGTTG+AGG 0.555557 8_4:+2478239 Msa1307150:CDS
CTTCAAGGAAATTGTTGAGG+TGG 0.564269 8_4:+2478242 Msa1307150:CDS
CATCATCATCAACTACATCA+TGG 0.574853 8_4:-2478188 None:intergenic
GAATGAAGGCAAATGGCTCA+AGG 0.589675 8_4:+2478914 Msa1307150:CDS
ACTAGCTAGTGTTAAGCCAT+GGG 0.596171 8_4:+2480265 Msa1307150:exon
AGATAACAGTGACACTCCCA+TGG 0.629854 8_4:-2480281 None:intergenic
TCTTAACATCAGAATCAGCA+AGG 0.653265 8_4:-2478347 None:intergenic
ACACAAATTACAAAATCACA+TGG 0.699258 8_4:-2480384 None:intergenic
GATTTGTCATGAAATCATGG+AGG 0.748556 8_4:+2478272 Msa1307150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAATTGAAAAAATTTAATTT+GGG - chr8_4:2480001-2480020 None:intergenic 10.0%
!! TGAATTGAAAAAATTTAATT+TGG - chr8_4:2480002-2480021 None:intergenic 10.0%
!!! AATTAAATTTTTTCAATTCA+TGG + chr8_4:2480002-2480021 Msa1307150:intron 10.0%
!!! ATCATATATTATGAAATTTT+TGG + chr8_4:2480206-2480225 Msa1307150:intron 10.0%
!!! TCATATATTATGAAATTTTT+GGG + chr8_4:2480207-2480226 Msa1307150:intron 10.0%
!!! GAATTGAAAAAATTTAATTT+GGG - chr8_4:2480001-2480020 None:intergenic 10.0%
!! TGAATTGAAAAAATTTAATT+TGG - chr8_4:2480002-2480021 None:intergenic 10.0%
!!! AATTAAATTTTTTCAATTCA+TGG + chr8_4:2480002-2480021 Msa1307150:intron 10.0%
!!! ATCATATATTATGAAATTTT+TGG + chr8_4:2480206-2480225 Msa1307150:intron 10.0%
!!! TCATATATTATGAAATTTTT+GGG + chr8_4:2480207-2480226 Msa1307150:intron 10.0%
!! TCAAGCAAATAATTAATTAT+TGG - chr8_4:2478766-2478785 None:intergenic 15.0%
!! ATTATTTGCTTGAAATTAAT+TGG + chr8_4:2478773-2478792 Msa1307150:intron 15.0%
!!! CTAAATAATCATTTGATTTT+AGG + chr8_4:2479189-2479208 Msa1307150:intron 15.0%
!!! TGTTTTTAAGATAAATAGAT+AGG + chr8_4:2479461-2479480 Msa1307150:intron 15.0%
!! TATATGGTTAATGTTTAATT+TGG + chr8_4:2480472-2480491 Msa1307150:three_prime_UTR 15.0%
!! TCAAGCAAATAATTAATTAT+TGG - chr8_4:2478766-2478785 None:intergenic 15.0%
!! ATTATTTGCTTGAAATTAAT+TGG + chr8_4:2478773-2478792 Msa1307150:intron 15.0%
!!! CTAAATAATCATTTGATTTT+AGG + chr8_4:2479189-2479208 Msa1307150:intron 15.0%
!!! TGTTTTTAAGATAAATAGAT+AGG + chr8_4:2479461-2479480 Msa1307150:intron 15.0%
!! TATATGGTTAATGTTTAATT+TGG + chr8_4:2480472-2480491 Msa1307150:three_prime_UTR 15.0%
!! ATTACATATATATAGCCTTT+AGG - chr8_4:2478544-2478563 None:intergenic 20.0%
!! ATATTATCATTTACTTGTGA+TGG + chr8_4:2479001-2479020 Msa1307150:intron 20.0%
!! GTAAATTCTTAGATACTTTA+GGG + chr8_4:2479211-2479230 Msa1307150:intron 20.0%
!! CTATTTATCTTAAAAACAGA+AGG - chr8_4:2479459-2479478 None:intergenic 20.0%
!! AAGATAAATAGATAGGTATA+TGG + chr8_4:2479468-2479487 Msa1307150:intron 20.0%
!!! AGTGTTGATTTTTTTTACAA+AGG - chr8_4:2479529-2479548 None:intergenic 20.0%
!! AATAATACTAAGTGAATGTT+TGG - chr8_4:2479922-2479941 None:intergenic 20.0%
!!! AAAAATTTAATTTGGGAAAC+AGG - chr8_4:2479994-2480013 None:intergenic 20.0%
!!! CTATAAATTTTGTCAAAAAG+AGG - chr8_4:2480133-2480152 None:intergenic 20.0%
!!! ATGAAATTTTTGGGTTTATT+TGG + chr8_4:2480216-2480235 Msa1307150:intron 20.0%
!! ATTACATATATATAGCCTTT+AGG - chr8_4:2478544-2478563 None:intergenic 20.0%
!! ATATTATCATTTACTTGTGA+TGG + chr8_4:2479001-2479020 Msa1307150:intron 20.0%
!! GTAAATTCTTAGATACTTTA+GGG + chr8_4:2479211-2479230 Msa1307150:intron 20.0%
!! CTATTTATCTTAAAAACAGA+AGG - chr8_4:2479459-2479478 None:intergenic 20.0%
!! AAGATAAATAGATAGGTATA+TGG + chr8_4:2479468-2479487 Msa1307150:intron 20.0%
!!! AGTGTTGATTTTTTTTACAA+AGG - chr8_4:2479529-2479548 None:intergenic 20.0%
!! AATAATACTAAGTGAATGTT+TGG - chr8_4:2479922-2479941 None:intergenic 20.0%
!!! AAAAATTTAATTTGGGAAAC+AGG - chr8_4:2479994-2480013 None:intergenic 20.0%
!!! CTATAAATTTTGTCAAAAAG+AGG - chr8_4:2480133-2480152 None:intergenic 20.0%
!!! ATGAAATTTTTGGGTTTATT+TGG + chr8_4:2480216-2480235 Msa1307150:intron 20.0%
! ATGATTTCATGACAAATCAT+AGG - chr8_4:2478270-2478289 None:intergenic 25.0%
!! TATGATTTGTCATGAAATCA+TGG + chr8_4:2478269-2478288 Msa1307150:CDS 25.0%
! AGTGAAAAACCAAAACTTAA+AGG + chr8_4:2478411-2478430 Msa1307150:CDS 25.0%
!!! CCATTTTTTCCTTTAAGTTT+TGG - chr8_4:2478423-2478442 None:intergenic 25.0%
! CCAAAACTTAAAGGAAAAAA+TGG + chr8_4:2478420-2478439 Msa1307150:CDS 25.0%
!! ATTTACTTCCATTTTCACAA+TGG + chr8_4:2478650-2478669 Msa1307150:intron 25.0%
! TTGCTTGAAATTAATTGGTT+AGG + chr8_4:2478778-2478797 Msa1307150:intron 25.0%
! ATTAATAACCACATGAATGA+AGG + chr8_4:2478900-2478919 Msa1307150:CDS 25.0%
! AAAAAGACAGTTCATGAAAA+GGG - chr8_4:2478980-2478999 None:intergenic 25.0%
! AAAAAAGACAGTTCATGAAA+AGG - chr8_4:2478981-2479000 None:intergenic 25.0%
! GGTAAATTCTTAGATACTTT+AGG + chr8_4:2479210-2479229 Msa1307150:intron 25.0%
!! CTGTATCATTCCAATTTTAT+CGG + chr8_4:2479314-2479333 Msa1307150:intron 25.0%
! ATTAATTTATAGTCCTTTCG+AGG - chr8_4:2479344-2479363 None:intergenic 25.0%
! TTTATCTTAAAAACAGAAGG+AGG - chr8_4:2479456-2479475 None:intergenic 25.0%
!!! AGTTTGAGTGTTTTTTTTAG+GGG - chr8_4:2479562-2479581 None:intergenic 25.0%
!!! CAGTTTGAGTGTTTTTTTTA+GGG - chr8_4:2479563-2479582 None:intergenic 25.0%
! CTAAAAAAAACACTCAAACT+GGG + chr8_4:2479562-2479581 Msa1307150:intron 25.0%
!! CAAACATGATTTTATAGAGT+TGG - chr8_4:2479715-2479734 None:intergenic 25.0%
! CAACTCTATAAAATCATGTT+TGG + chr8_4:2479713-2479732 Msa1307150:intron 25.0%
! TCAATATCACTTGAATAGAT+TGG - chr8_4:2480089-2480108 None:intergenic 25.0%
! ATCTATTCAAGTGATATTGA+TGG + chr8_4:2480089-2480108 Msa1307150:intron 25.0%
! TTTATTTGGATTAACCTTTC+TGG + chr8_4:2480230-2480249 Msa1307150:intron 25.0%
! ACGTATGAGATTAAATACTT+AGG + chr8_4:2480327-2480346 Msa1307150:three_prime_UTR 25.0%
! ACACAAATTACAAAATCACA+TGG - chr8_4:2480387-2480406 None:intergenic 25.0%
! GTTATCTGTGACTATATATA+TGG + chr8_4:2480456-2480475 Msa1307150:three_prime_UTR 25.0%
! ATGATTTCATGACAAATCAT+AGG - chr8_4:2478270-2478289 None:intergenic 25.0%
!! TATGATTTGTCATGAAATCA+TGG + chr8_4:2478269-2478288 Msa1307150:CDS 25.0%
! AGTGAAAAACCAAAACTTAA+AGG + chr8_4:2478411-2478430 Msa1307150:CDS 25.0%
!!! CCATTTTTTCCTTTAAGTTT+TGG - chr8_4:2478423-2478442 None:intergenic 25.0%
! CCAAAACTTAAAGGAAAAAA+TGG + chr8_4:2478420-2478439 Msa1307150:CDS 25.0%
!! ATTTACTTCCATTTTCACAA+TGG + chr8_4:2478650-2478669 Msa1307150:intron 25.0%
! TTGCTTGAAATTAATTGGTT+AGG + chr8_4:2478778-2478797 Msa1307150:intron 25.0%
! ATTAATAACCACATGAATGA+AGG + chr8_4:2478900-2478919 Msa1307150:CDS 25.0%
! AAAAAGACAGTTCATGAAAA+GGG - chr8_4:2478980-2478999 None:intergenic 25.0%
! AAAAAAGACAGTTCATGAAA+AGG - chr8_4:2478981-2479000 None:intergenic 25.0%
! GGTAAATTCTTAGATACTTT+AGG + chr8_4:2479210-2479229 Msa1307150:intron 25.0%
!! CTGTATCATTCCAATTTTAT+CGG + chr8_4:2479314-2479333 Msa1307150:intron 25.0%
! ATTAATTTATAGTCCTTTCG+AGG - chr8_4:2479344-2479363 None:intergenic 25.0%
! TTTATCTTAAAAACAGAAGG+AGG - chr8_4:2479456-2479475 None:intergenic 25.0%
!!! AGTTTGAGTGTTTTTTTTAG+GGG - chr8_4:2479562-2479581 None:intergenic 25.0%
!!! CAGTTTGAGTGTTTTTTTTA+GGG - chr8_4:2479563-2479582 None:intergenic 25.0%
! CTAAAAAAAACACTCAAACT+GGG + chr8_4:2479562-2479581 Msa1307150:intron 25.0%
!! CAAACATGATTTTATAGAGT+TGG - chr8_4:2479715-2479734 None:intergenic 25.0%
! CAACTCTATAAAATCATGTT+TGG + chr8_4:2479713-2479732 Msa1307150:intron 25.0%
! TCAATATCACTTGAATAGAT+TGG - chr8_4:2480089-2480108 None:intergenic 25.0%
! ATCTATTCAAGTGATATTGA+TGG + chr8_4:2480089-2480108 Msa1307150:intron 25.0%
! TTTATTTGGATTAACCTTTC+TGG + chr8_4:2480230-2480249 Msa1307150:intron 25.0%
! ACGTATGAGATTAAATACTT+AGG + chr8_4:2480327-2480346 Msa1307150:three_prime_UTR 25.0%
! ACACAAATTACAAAATCACA+TGG - chr8_4:2480387-2480406 None:intergenic 25.0%
! GTTATCTGTGACTATATATA+TGG + chr8_4:2480456-2480475 Msa1307150:three_prime_UTR 25.0%
ATTTGATGAGAACAACTTTG+AGG + chr8_4:2478149-2478168 Msa1307150:five_prime_UTR 30.0%
AAAGAGGAAGAAATGATGAT+TGG + chr8_4:2478216-2478235 Msa1307150:exon 30.0%
AATGATGATTGGAAACTTCA+AGG + chr8_4:2478227-2478246 Msa1307150:exon 30.0%
AAACTTCAAGGAAATTGTTG+AGG + chr8_4:2478239-2478258 Msa1307150:CDS 30.0%
TTGAAGCTGATCAAAAGAAA+AGG + chr8_4:2478304-2478323 Msa1307150:CDS 30.0%
ACTTTCTTGATGTCTTTCAT+TGG - chr8_4:2478495-2478514 None:intergenic 30.0%
! TATCATTTACTTGTGATGGA+AGG + chr8_4:2479005-2479024 Msa1307150:intron 30.0%
! ATGATAACCTTTTCAACCTA+AGG - chr8_4:2479125-2479144 None:intergenic 30.0%
CGATAAAATTGGAATGATAC+AGG - chr8_4:2479316-2479335 None:intergenic 30.0%
TACATACACAATTAAACTCG+AGG + chr8_4:2479403-2479422 Msa1307150:intron 30.0%
ACAGAAGGAGGATAAAATTA+AGG - chr8_4:2479444-2479463 None:intergenic 30.0%
ATAGATAGGTATATGGATGA+TGG + chr8_4:2479475-2479494 Msa1307150:intron 30.0%
!!! CCAGTTTGAGTGTTTTTTTT+AGG - chr8_4:2479564-2479583 None:intergenic 30.0%
CCTAAAAAAAACACTCAAAC+TGG + chr8_4:2479561-2479580 Msa1307150:intron 30.0%
CCAAGAAAAAAGAGTTTAAG+AGG - chr8_4:2479589-2479608 None:intergenic 30.0%
!!! CCTCTTAAACTCTTTTTTCT+TGG + chr8_4:2479586-2479605 Msa1307150:intron 30.0%
TAACATCCTATCAATTAAGC+AGG + chr8_4:2479613-2479632 Msa1307150:intron 30.0%
ATGTATCCTGCTTAATTGAT+AGG - chr8_4:2479622-2479641 None:intergenic 30.0%
! TTATAGAGTTGGAATGCTTT+TGG - chr8_4:2479704-2479723 None:intergenic 30.0%
!!! ATGTTTTAGGGTAATTCTGT+GGG - chr8_4:2480034-2480053 None:intergenic 30.0%
!! CTCTTTGTTTGGATGTTTTA+GGG - chr8_4:2480046-2480065 None:intergenic 30.0%
AATCACATGGTTCAATTCTT+TGG - chr8_4:2480374-2480393 None:intergenic 30.0%
ATTTGATGAGAACAACTTTG+AGG + chr8_4:2478149-2478168 Msa1307150:five_prime_UTR 30.0%
AAAGAGGAAGAAATGATGAT+TGG + chr8_4:2478216-2478235 Msa1307150:exon 30.0%
AATGATGATTGGAAACTTCA+AGG + chr8_4:2478227-2478246 Msa1307150:exon 30.0%
AAACTTCAAGGAAATTGTTG+AGG + chr8_4:2478239-2478258 Msa1307150:CDS 30.0%
TTGAAGCTGATCAAAAGAAA+AGG + chr8_4:2478304-2478323 Msa1307150:CDS 30.0%
ACTTTCTTGATGTCTTTCAT+TGG - chr8_4:2478495-2478514 None:intergenic 30.0%
! TATCATTTACTTGTGATGGA+AGG + chr8_4:2479005-2479024 Msa1307150:intron 30.0%
! ATGATAACCTTTTCAACCTA+AGG - chr8_4:2479125-2479144 None:intergenic 30.0%
CGATAAAATTGGAATGATAC+AGG - chr8_4:2479316-2479335 None:intergenic 30.0%
TACATACACAATTAAACTCG+AGG + chr8_4:2479403-2479422 Msa1307150:intron 30.0%
ACAGAAGGAGGATAAAATTA+AGG - chr8_4:2479444-2479463 None:intergenic 30.0%
ATAGATAGGTATATGGATGA+TGG + chr8_4:2479475-2479494 Msa1307150:intron 30.0%
!!! CCAGTTTGAGTGTTTTTTTT+AGG - chr8_4:2479564-2479583 None:intergenic 30.0%
CCTAAAAAAAACACTCAAAC+TGG + chr8_4:2479561-2479580 Msa1307150:intron 30.0%
CCAAGAAAAAAGAGTTTAAG+AGG - chr8_4:2479589-2479608 None:intergenic 30.0%
!!! CCTCTTAAACTCTTTTTTCT+TGG + chr8_4:2479586-2479605 Msa1307150:intron 30.0%
TAACATCCTATCAATTAAGC+AGG + chr8_4:2479613-2479632 Msa1307150:intron 30.0%
ATGTATCCTGCTTAATTGAT+AGG - chr8_4:2479622-2479641 None:intergenic 30.0%
! TTATAGAGTTGGAATGCTTT+TGG - chr8_4:2479704-2479723 None:intergenic 30.0%
!!! ATGTTTTAGGGTAATTCTGT+GGG - chr8_4:2480034-2480053 None:intergenic 30.0%
!! CTCTTTGTTTGGATGTTTTA+GGG - chr8_4:2480046-2480065 None:intergenic 30.0%
AATCACATGGTTCAATTCTT+TGG - chr8_4:2480374-2480393 None:intergenic 30.0%
CATCATCATCAACTACATCA+TGG - chr8_4:2478191-2478210 None:intergenic 35.0%
! TGATGATGATGCTAGAAAAG+AGG + chr8_4:2478200-2478219 Msa1307150:five_prime_UTR 35.0%
GATTTGTCATGAAATCATGG+AGG + chr8_4:2478272-2478291 Msa1307150:CDS 35.0%
TCTTAACATCAGAATCAGCA+AGG - chr8_4:2478350-2478369 None:intergenic 35.0%
GAAGATTATTCCCCTTGTTA+AGG + chr8_4:2478458-2478477 Msa1307150:CDS 35.0%
! ACGTGCATTTTCCTTAACAA+GGG - chr8_4:2478472-2478491 None:intergenic 35.0%
CATTGACACCATTGTGAAAA+TGG - chr8_4:2478661-2478680 None:intergenic 35.0%
TGATGAAGAAGATGCTAAAG+AGG + chr8_4:2478826-2478845 Msa1307150:CDS 35.0%
TGATTCTTGACATCAAGATC+TGG - chr8_4:2478861-2478880 None:intergenic 35.0%
! AGGTCTTTTATCGCAGTTTA+AGG + chr8_4:2478934-2478953 Msa1307150:CDS 35.0%
ACATGACAAACACATACCTT+AGG + chr8_4:2479106-2479125 Msa1307150:intron 35.0%
!! ACACATACCTTAGGTTGAAA+AGG + chr8_4:2479115-2479134 Msa1307150:intron 35.0%
!! ACTTTAGGGTTTAGTCGTAT+CGG + chr8_4:2479225-2479244 Msa1307150:intron 35.0%
TTTCAGTGAAGATAGATGCA+GGG + chr8_4:2479960-2479979 Msa1307150:intron 35.0%
!!! GATGTTTTAGGGTAATTCTG+TGG - chr8_4:2480035-2480054 None:intergenic 35.0%
!! CCTCTTTGTTTGGATGTTTT+AGG - chr8_4:2480047-2480066 None:intergenic 35.0%
CCTAAAACATCCAAACAAAG+AGG + chr8_4:2480044-2480063 Msa1307150:intron 35.0%
GCTATATGAACCTCTTTGTT+TGG - chr8_4:2480057-2480076 None:intergenic 35.0%
!! TGTGTTCATGTTGTTTCTGT+TGG + chr8_4:2480404-2480423 Msa1307150:three_prime_UTR 35.0%
CATCATCATCAACTACATCA+TGG - chr8_4:2478191-2478210 None:intergenic 35.0%
! TGATGATGATGCTAGAAAAG+AGG + chr8_4:2478200-2478219 Msa1307150:five_prime_UTR 35.0%
GATTTGTCATGAAATCATGG+AGG + chr8_4:2478272-2478291 Msa1307150:CDS 35.0%
TCTTAACATCAGAATCAGCA+AGG - chr8_4:2478350-2478369 None:intergenic 35.0%
GAAGATTATTCCCCTTGTTA+AGG + chr8_4:2478458-2478477 Msa1307150:CDS 35.0%
! ACGTGCATTTTCCTTAACAA+GGG - chr8_4:2478472-2478491 None:intergenic 35.0%
CATTGACACCATTGTGAAAA+TGG - chr8_4:2478661-2478680 None:intergenic 35.0%
TGATGAAGAAGATGCTAAAG+AGG + chr8_4:2478826-2478845 Msa1307150:CDS 35.0%
TGATTCTTGACATCAAGATC+TGG - chr8_4:2478861-2478880 None:intergenic 35.0%
! AGGTCTTTTATCGCAGTTTA+AGG + chr8_4:2478934-2478953 Msa1307150:CDS 35.0%
ACATGACAAACACATACCTT+AGG + chr8_4:2479106-2479125 Msa1307150:intron 35.0%
!! ACACATACCTTAGGTTGAAA+AGG + chr8_4:2479115-2479134 Msa1307150:intron 35.0%
!! ACTTTAGGGTTTAGTCGTAT+CGG + chr8_4:2479225-2479244 Msa1307150:intron 35.0%
TTTCAGTGAAGATAGATGCA+GGG + chr8_4:2479960-2479979 Msa1307150:intron 35.0%
!!! GATGTTTTAGGGTAATTCTG+TGG - chr8_4:2480035-2480054 None:intergenic 35.0%
!! CCTCTTTGTTTGGATGTTTT+AGG - chr8_4:2480047-2480066 None:intergenic 35.0%
CCTAAAACATCCAAACAAAG+AGG + chr8_4:2480044-2480063 Msa1307150:intron 35.0%
GCTATATGAACCTCTTTGTT+TGG - chr8_4:2480057-2480076 None:intergenic 35.0%
!! TGTGTTCATGTTGTTTCTGT+TGG + chr8_4:2480404-2480423 Msa1307150:three_prime_UTR 35.0%
CTTCAAGGAAATTGTTGAGG+TGG + chr8_4:2478242-2478261 Msa1307150:CDS 40.0%
TTATTCTGCAGCCAAACTCT+TGG + chr8_4:2478380-2478399 Msa1307150:CDS 40.0%
ACTTGACTCAACCAAGAGTT+TGG - chr8_4:2478394-2478413 None:intergenic 40.0%
! CGTGCATTTTCCTTAACAAG+GGG - chr8_4:2478471-2478490 None:intergenic 40.0%
! GACGTGCATTTTCCTTAACA+AGG - chr8_4:2478473-2478492 None:intergenic 40.0%
CTACATCATGCACTTCCTAA+AGG + chr8_4:2478526-2478545 Msa1307150:intron 40.0%
GTATCTGTGTCAATGCTTCA+TGG + chr8_4:2478697-2478716 Msa1307150:intron 40.0%
ACCACATGAATGAAGGCAAA+TGG + chr8_4:2478907-2478926 Msa1307150:CDS 40.0%
! TACTTGTGATGGAAGGTTTG+TGG + chr8_4:2479012-2479031 Msa1307150:intron 40.0%
TCGAGGACATCCGATAAAAT+TGG - chr8_4:2479327-2479346 None:intergenic 40.0%
! TTTTATCGGATGTCCTCGAA+AGG + chr8_4:2479328-2479347 Msa1307150:intron 40.0%
CTTTCAGTGAAGATAGATGC+AGG + chr8_4:2479959-2479978 Msa1307150:intron 40.0%
TTAGGGTAATTCTGTGGGAT+GGG - chr8_4:2480029-2480048 None:intergenic 40.0%
! TTTAGGGTAATTCTGTGGGA+TGG - chr8_4:2480030-2480049 None:intergenic 40.0%
!! TAACCTTTCTGGTTTGTTGC+AGG + chr8_4:2480241-2480260 Msa1307150:intron 40.0%
TACTAGCTAGTGTTAAGCCA+TGG + chr8_4:2480264-2480283 Msa1307150:exon 40.0%
ACTAGCTAGTGTTAAGCCAT+GGG + chr8_4:2480265-2480284 Msa1307150:exon 40.0%
CTTCAAGGAAATTGTTGAGG+TGG + chr8_4:2478242-2478261 Msa1307150:CDS 40.0%
TTATTCTGCAGCCAAACTCT+TGG + chr8_4:2478380-2478399 Msa1307150:CDS 40.0%
ACTTGACTCAACCAAGAGTT+TGG - chr8_4:2478394-2478413 None:intergenic 40.0%
! CGTGCATTTTCCTTAACAAG+GGG - chr8_4:2478471-2478490 None:intergenic 40.0%
! GACGTGCATTTTCCTTAACA+AGG - chr8_4:2478473-2478492 None:intergenic 40.0%
CTACATCATGCACTTCCTAA+AGG + chr8_4:2478526-2478545 Msa1307150:intron 40.0%
GTATCTGTGTCAATGCTTCA+TGG + chr8_4:2478697-2478716 Msa1307150:intron 40.0%
ACCACATGAATGAAGGCAAA+TGG + chr8_4:2478907-2478926 Msa1307150:CDS 40.0%
! TACTTGTGATGGAAGGTTTG+TGG + chr8_4:2479012-2479031 Msa1307150:intron 40.0%
TCGAGGACATCCGATAAAAT+TGG - chr8_4:2479327-2479346 None:intergenic 40.0%
! TTTTATCGGATGTCCTCGAA+AGG + chr8_4:2479328-2479347 Msa1307150:intron 40.0%
CTTTCAGTGAAGATAGATGC+AGG + chr8_4:2479959-2479978 Msa1307150:intron 40.0%
TTAGGGTAATTCTGTGGGAT+GGG - chr8_4:2480029-2480048 None:intergenic 40.0%
! TTTAGGGTAATTCTGTGGGA+TGG - chr8_4:2480030-2480049 None:intergenic 40.0%
!! TAACCTTTCTGGTTTGTTGC+AGG + chr8_4:2480241-2480260 Msa1307150:intron 40.0%
TACTAGCTAGTGTTAAGCCA+TGG + chr8_4:2480264-2480283 Msa1307150:exon 40.0%
ACTAGCTAGTGTTAAGCCAT+GGG + chr8_4:2480265-2480284 Msa1307150:exon 40.0%
GCTTCATGGTTTGATCAGTG+AGG + chr8_4:2478711-2478730 Msa1307150:intron 45.0%
GCCATTTGCCTTCATTCATG+TGG - chr8_4:2478911-2478930 None:intergenic 45.0%
GAATGAAGGCAAATGGCTCA+AGG + chr8_4:2478914-2478933 Msa1307150:CDS 45.0%
GTACCTGCAACAAACCAGAA+AGG - chr8_4:2480247-2480266 None:intergenic 45.0%
AGATAACAGTGACACTCCCA+TGG - chr8_4:2480284-2480303 None:intergenic 45.0%
!! ATGGGAGTGTCACTGTTATC+TGG + chr8_4:2480283-2480302 Msa1307150:three_prime_UTR 45.0%
GCTTCATGGTTTGATCAGTG+AGG + chr8_4:2478711-2478730 Msa1307150:intron 45.0%
GCCATTTGCCTTCATTCATG+TGG - chr8_4:2478911-2478930 None:intergenic 45.0%
GAATGAAGGCAAATGGCTCA+AGG + chr8_4:2478914-2478933 Msa1307150:CDS 45.0%
GTACCTGCAACAAACCAGAA+AGG - chr8_4:2480247-2480266 None:intergenic 45.0%
AGATAACAGTGACACTCCCA+TGG - chr8_4:2480284-2480303 None:intergenic 45.0%
!! ATGGGAGTGTCACTGTTATC+TGG + chr8_4:2480283-2480302 Msa1307150:three_prime_UTR 45.0%
!!! CAAATAAATTTATTTTTTTT+TGG - chr8_4:2479830-2479849 None:intergenic 5.0%
!!! CAAATAAATTTATTTTTTTT+TGG - chr8_4:2479830-2479849 None:intergenic 5.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 2478138 2480495 2478138 ID=Msa1307150;Name=Msa1307150
chr8_4 mRNA 2478138 2480495 2478138 ID=Msa1307150-mRNA-1;Parent=Msa1307150;Name=Msa1307150-mRNA-1;_AED=0.02;_eAED=0.02;_QI=90|1|1|1|0.5|0.33|3|226|140
chr8_4 exon 2478138 2478512 2478138 ID=Msa1307150-mRNA-1:exon:774;Parent=Msa1307150-mRNA-1
chr8_4 exon 2478825 2478955 2478825 ID=Msa1307150-mRNA-1:exon:775;Parent=Msa1307150-mRNA-1
chr8_4 exon 2480263 2480495 2480263 ID=Msa1307150-mRNA-1:exon:776;Parent=Msa1307150-mRNA-1
chr8_4 five_prime_UTR 2478138 2478227 2478138 ID=Msa1307150-mRNA-1:five_prime_utr;Parent=Msa1307150-mRNA-1
chr8_4 CDS 2478228 2478512 2478228 ID=Msa1307150-mRNA-1:cds;Parent=Msa1307150-mRNA-1
chr8_4 CDS 2478825 2478955 2478825 ID=Msa1307150-mRNA-1:cds;Parent=Msa1307150-mRNA-1
chr8_4 CDS 2480263 2480269 2480263 ID=Msa1307150-mRNA-1:cds;Parent=Msa1307150-mRNA-1
chr8_4 three_prime_UTR 2480270 2480495 2480270 ID=Msa1307150-mRNA-1:three_prime_utr;Parent=Msa1307150-mRNA-1
Gene Sequence

>Msa1307150

ATGATGATTGGAAACTTCAAGGAAATTGTTGAGGTGGCACCTATGATTTGTCATGAAATCATGGAGGCAAATGATGTTGAAGCTGATCAAAAGAAAAGGATCACATTAGCTGATCTATTCCTTGCTGATTCTGATGTTAAGATGAAACTTGATTATTCTGCAGCCAAACTCTTGGTTGAGTCAAGTGAAAAACCAAAACTTAAAGGAAAAAATGGATTTTCTTTTGCTAAGAAGATTATTCCCCTTGTTAAGGAAAATGCACGTCCAATGAAAGACATCAAGAAACTGATGAAGAAGATGCTAAAGAGGAAAATTCATCCAGATCTTGATGTCAAGAATCACAAAGCAGAAGAAAACATAATTAATAACCACATGAATGAAGGCAAATGGCTCAAGGTCTTTTATCGCAGTTTAAGGTACTAG
Protein sequence

>Msa1307150

MMIGNFKEIVEVAPMICHEIMEANDVEADQKKRITLADLFLADSDVKMKLDYSAAKLLVESSEKPKLKGKNGFSFAKKIIPLVKENARPMKDIKKLMKKMLKRKIHPDLDVKNHKAEENIINNHMNEGKWLKVFYRSLRY*