Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1356990 | AES61501.1 | 93.827 | 81 | 5 | 0 | 1 | 81 | 1 | 81 | 3.99e-49 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1356990 | G7IAC0 | 93.827 | 81 | 5 | 0 | 1 | 81 | 1 | 81 | 1.91e-49 | 159 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0080990 | Msa1356990 | 0.971296 | 6.097041e-133 | -8.615850e-47 |
| Msa0109880 | Msa1356990 | 0.809345 | 1.981149e-50 | -8.615850e-47 |
| Msa1356990 | Msa1379970 | 0.836463 | 9.219155e-57 | -8.615850e-47 |
| Msa1356990 | Msa1406780 | 0.827516 | 1.498762e-54 | -8.615850e-47 |
| Msa1356990 | Msa1421550 | 0.828226 | 1.011206e-54 | -8.615850e-47 |
| Msa1356990 | Msa1431850 | 0.836749 | 7.796136e-57 | -8.615850e-47 |
| Msa1356990 | Msa1443140 | 0.813702 | 2.238662e-51 | -8.615850e-47 |
| Msa0739580 | Msa1356990 | 0.835666 | 1.468975e-56 | -8.615850e-47 |
| Msa0748000 | Msa1356990 | 0.971334 | 5.323593e-133 | -8.615850e-47 |
| Msa0782360 | Msa1356990 | 0.805929 | 1.052669e-49 | -8.615850e-47 |
| Msa0828830 | Msa1356990 | 0.811633 | 6.350371e-51 | -8.615850e-47 |
| Msa0124130 | Msa1356990 | 0.816027 | 6.833021e-52 | -8.615850e-47 |
| Msa0149140 | Msa1356990 | 0.804680 | 1.923306e-49 | -8.615850e-47 |
| Msa0156250 | Msa1356990 | 0.822773 | 1.979550e-53 | -8.615850e-47 |
| Msa0212150 | Msa1356990 | 0.829728 | 4.375003e-55 | -8.615850e-47 |
| Msa0221290 | Msa1356990 | 0.809736 | 1.632584e-50 | -8.615850e-47 |
| Msa1228190 | Msa1356990 | 0.853606 | 2.137854e-61 | -8.615850e-47 |
| Msa1271260 | Msa1356990 | 0.814341 | 1.618673e-51 | -8.615850e-47 |
| Msa1277400 | Msa1356990 | 0.807432 | 5.070362e-50 | -8.615850e-47 |
| Msa1305310 | Msa1356990 | 0.800903 | 1.158283e-48 | -8.615850e-47 |
| Msa1307150 | Msa1356990 | 0.814698 | 1.349553e-51 | -8.615850e-47 |
| Msa1307190 | Msa1356990 | 0.820331 | 7.258626e-53 | -8.615850e-47 |
| Msa1339580 | Msa1356990 | 0.961011 | 3.286301e-119 | -8.615850e-47 |
| Msa0406000 | Msa1356990 | 0.822403 | 2.413163e-53 | -8.615850e-47 |
| Msa0451400 | Msa1356990 | 0.822302 | 2.547035e-53 | -8.615850e-47 |
| Msa0999300 | Msa1356990 | 0.845999 | 2.859446e-59 | -8.615850e-47 |
| Msa1001890 | Msa1356990 | 0.829171 | 5.976496e-55 | -8.615850e-47 |
| Msa0252770 | Msa1356990 | 0.856200 | 3.777154e-62 | -8.615850e-47 |
| Msa0289920 | Msa1356990 | 0.829370 | 5.348312e-55 | -8.615850e-47 |
| Msa0306120 | Msa1356990 | 0.840545 | 8.154686e-58 | -8.615850e-47 |
| Msa0329620 | Msa1356990 | 0.876778 | 1.065345e-68 | -8.615850e-47 |
| Msa0359250 | Msa1356990 | 0.825710 | 4.040741e-54 | -8.615850e-47 |
| Msa1176160 | Msa1356990 | 0.817169 | 3.790793e-52 | -8.615850e-47 |
| Msa0492190 | Msa1356990 | 0.807051 | 6.104575e-50 | -8.615850e-47 |
| Msa0860280 | Msa1356990 | 0.817207 | 3.717206e-52 | -8.615850e-47 |
| Msa0929870 | Msa1356990 | 0.803636 | 3.171251e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1356990 | MtrunA17_Chr1g0193271 | 80.723 | 83 | 14 | 1 | 1 | 81 | 1 | 83 | 7.35e-43 | 134 |
| Msa1356990 | MtrunA17_Chr1g0193251 | 83.750 | 80 | 13 | 0 | 2 | 81 | 4 | 83 | 5.68e-36 | 116 |
| Msa1356990 | MtrunA17_Chr7g0271321 | 48.101 | 79 | 38 | 2 | 1 | 76 | 1 | 79 | 4.12e-19 | 73.9 |
| Msa1356990 | MtrunA17_Chr1g0193261 | 56.923 | 65 | 22 | 3 | 15 | 73 | 18 | 82 | 2.23e-17 | 69.7 |
| Msa1356990 | MtrunA17_Chr1g0193231 | 56.923 | 65 | 22 | 3 | 15 | 73 | 18 | 82 | 2.25e-17 | 69.7 |
| Msa1356990 | MtrunA17_Chr1g0193301 | 56.923 | 65 | 22 | 3 | 15 | 73 | 19 | 83 | 2.63e-17 | 69.7 |
| Msa1356990 | MtrunA17_Chr1g0193291 | 59.016 | 61 | 20 | 3 | 18 | 73 | 20 | 80 | 1.01e-15 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1356990 | AT3G05858.1 | 45.882 | 85 | 39 | 4 | 1 | 79 | 1 | 84 | 2.57e-12 | 57.4 |
Find 16 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGATGAAGACAAAGGTAAC+TGG | 0.348340 | tig0015879:-58634 | Msa1356990:CDS |
| GGTAAGACTAAGTCTTCTTC+AGG | 0.365971 | tig0015879:-58743 | Msa1356990:CDS |
| TCAGCTCTCATGTTAATGTT+AGG | 0.378896 | tig0015879:+58599 | None:intergenic |
| AAACTCGATTCTTGTATTTG+CGG | 0.401356 | tig0015879:+58567 | None:intergenic |
| TGGATCAACTTCATAGCGTT+CGG | 0.459202 | tig0015879:+58538 | None:intergenic |
| TTACCTTTGTCTTCATCACT+AGG | 0.502543 | tig0015879:+58638 | None:intergenic |
| TATTATGCATTCTAGACCGC+TGG | 0.510025 | tig0015879:+58518 | None:intergenic |
| TCAAAGAATAAGTCTAGAGC+AGG | 0.540317 | tig0015879:-58692 | Msa1356990:CDS |
| TGATGAAGACAAAGGTAACT+GGG | 0.585582 | tig0015879:-58633 | Msa1356990:CDS |
| GCATTAATCATCATGGGAGG+TGG | 0.588766 | tig0015879:-58764 | None:intergenic |
| GCAGGATACTATGATGATTG+CGG | 0.626514 | tig0015879:-58674 | Msa1356990:CDS |
| ATGATTGCGGATGCAAGACA+TGG | 0.656100 | tig0015879:-58661 | Msa1356990:CDS |
| ACGCTATGAAGTTGATCCAG+CGG | 0.687155 | tig0015879:-58534 | Msa1356990:CDS |
| TGGCCTAGTGATGAAGACAA+AGG | 0.708445 | tig0015879:-58641 | Msa1356990:CDS |
| GCAGCATTAATCATCATGGG+AGG | 0.708531 | tig0015879:-58767 | None:intergenic |
| GATGAAGACAAAGGTAACTG+GGG | 0.719672 | tig0015879:-58632 | Msa1356990:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACTCGATTCTTGTATTTG+CGG | + | tig0015879:58717-58736 | None:intergenic | 30.0% | |
| ! | TCAAAGAATAAGTCTAGAGC+AGG | - | tig0015879:58589-58608 | Msa1356990:CDS | 35.0% |
| TTACCTTTGTCTTCATCACT+AGG | + | tig0015879:58646-58665 | None:intergenic | 35.0% | |
| TGATGAAGACAAAGGTAACT+GGG | - | tig0015879:58648-58667 | Msa1356990:CDS | 35.0% | |
| TCAGCTCTCATGTTAATGTT+AGG | + | tig0015879:58685-58704 | None:intergenic | 35.0% | |
| GGTAAGACTAAGTCTTCTTC+AGG | - | tig0015879:58538-58557 | Msa1356990:CDS | 40.0% | |
| GCAGGATACTATGATGATTG+CGG | - | tig0015879:58607-58626 | Msa1356990:CDS | 40.0% | |
| GTGATGAAGACAAAGGTAAC+TGG | - | tig0015879:58647-58666 | Msa1356990:CDS | 40.0% | |
| GATGAAGACAAAGGTAACTG+GGG | - | tig0015879:58649-58668 | Msa1356990:CDS | 40.0% | |
| TGGATCAACTTCATAGCGTT+CGG | + | tig0015879:58746-58765 | None:intergenic | 40.0% | |
| ATGATTGCGGATGCAAGACA+TGG | - | tig0015879:58620-58639 | Msa1356990:CDS | 45.0% | |
| TGGCCTAGTGATGAAGACAA+AGG | - | tig0015879:58640-58659 | Msa1356990:CDS | 45.0% | |
| ! | ACGCTATGAAGTTGATCCAG+CGG | - | tig0015879:58747-58766 | Msa1356990:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0015879 | gene | 58529 | 58774 | 58529 | ID=Msa1356990;Name=Msa1356990 |
| tig0015879 | mRNA | 58529 | 58774 | 58529 | ID=Msa1356990-mRNA-1;Parent=Msa1356990;Name=Msa1356990-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|81 |
| tig0015879 | exon | 58529 | 58774 | 58529 | ID=Msa1356990-mRNA-1:exon:10228;Parent=Msa1356990-mRNA-1 |
| tig0015879 | CDS | 58529 | 58774 | 58529 | ID=Msa1356990-mRNA-1:cds;Parent=Msa1356990-mRNA-1 |
| Gene Sequence |
| Protein sequence |
