Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0080990 | AES61501.1 | 93.827 | 81 | 5 | 0 | 1 | 81 | 1 | 81 | 3.99e-49 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0080990 | G7IAC0 | 93.827 | 81 | 5 | 0 | 1 | 81 | 1 | 81 | 1.91e-49 | 159 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0006980 | Msa0080990 | 0.807281 | 5.456644e-50 | -8.615850e-47 |
| Msa0016120 | Msa0080990 | 0.802106 | 6.566167e-49 | -8.615850e-47 |
| Msa0080910 | Msa0080990 | 0.805325 | 1.409888e-49 | -8.615850e-47 |
| Msa0080990 | Msa0109880 | 0.820553 | 6.454082e-53 | -8.615850e-47 |
| Msa0080990 | Msa0124130 | 0.831595 | 1.526922e-55 | -8.615850e-47 |
| Msa0080990 | Msa0149140 | 0.813925 | 1.999664e-51 | -8.615850e-47 |
| Msa0080990 | Msa0156250 | 0.832979 | 6.940315e-56 | -8.615850e-47 |
| Msa0080990 | Msa0212150 | 0.844457 | 7.472839e-59 | -8.615850e-47 |
| Msa0080990 | Msa0221290 | 0.816686 | 4.866439e-52 | -8.615850e-47 |
| Msa0080990 | Msa0236110 | 0.801789 | 7.629169e-49 | -8.615850e-47 |
| Msa0080990 | Msa0252770 | 0.866515 | 2.695015e-65 | -8.615850e-47 |
| Msa0080990 | Msa0276080 | 0.819077 | 1.403366e-52 | -8.615850e-47 |
| Msa0080990 | Msa0289920 | 0.837982 | 3.768012e-57 | -8.615850e-47 |
| Msa0080990 | Msa0306120 | 0.844824 | 5.949421e-59 | -8.615850e-47 |
| Msa0080990 | Msa0314630 | 0.806048 | 9.940737e-50 | -8.615850e-47 |
| Msa0080990 | Msa0329620 | 0.884571 | 1.712075e-71 | -8.615850e-47 |
| Msa0080990 | Msa0359250 | 0.838529 | 2.723736e-57 | -8.615850e-47 |
| Msa0080990 | Msa0406000 | 0.837426 | 5.235421e-57 | -8.615850e-47 |
| Msa0080990 | Msa0424340 | 0.805110 | 1.563710e-49 | -8.615850e-47 |
| Msa0080990 | Msa0451400 | 0.837725 | 4.386495e-57 | -8.615850e-47 |
| Msa0080990 | Msa0492190 | 0.823018 | 1.736044e-53 | -8.615850e-47 |
| Msa0080990 | Msa0575740 | 0.817284 | 3.572024e-52 | -8.615850e-47 |
| Msa0080990 | Msa0641360 | 0.809129 | 2.203568e-50 | -8.615850e-47 |
| Msa0080990 | Msa0669840 | 0.808145 | 3.576144e-50 | -8.615850e-47 |
| Msa0080990 | Msa0682530 | 0.814498 | 1.493900e-51 | -8.615850e-47 |
| Msa0080990 | Msa0739580 | 0.841438 | 4.750487e-58 | -8.615850e-47 |
| Msa0080990 | Msa0748000 | 0.999706 | 0.000000e+00 | -8.615850e-47 |
| Msa0080990 | Msa0782360 | 0.811648 | 6.301065e-51 | -8.615850e-47 |
| Msa0080990 | Msa0828830 | 0.822498 | 2.294367e-53 | -8.615850e-47 |
| Msa0080990 | Msa0860280 | 0.823536 | 1.313669e-53 | -8.615850e-47 |
| Msa0080990 | Msa0929870 | 0.818144 | 2.284218e-52 | -8.615850e-47 |
| Msa0080990 | Msa0979510 | 0.800189 | 1.619270e-48 | -8.615850e-47 |
| Msa0080990 | Msa0999300 | 0.849686 | 2.756889e-60 | -8.615850e-47 |
| Msa0080990 | Msa0999350 | 0.826081 | 3.299437e-54 | -8.615850e-47 |
| Msa0080990 | Msa0999360 | 0.811488 | 6.828767e-51 | -8.615850e-47 |
| Msa0080990 | Msa0999400 | 0.818384 | 2.015845e-52 | -8.615850e-47 |
| Msa0080990 | Msa0999410 | 0.815787 | 7.730099e-52 | -8.615850e-47 |
| Msa0080990 | Msa1001890 | 0.832844 | 7.495563e-56 | -8.615850e-47 |
| Msa0080990 | Msa1034680 | 0.810010 | 1.425703e-50 | -8.615850e-47 |
| Msa0080990 | Msa1037770 | 0.805588 | 1.241732e-49 | -8.615850e-47 |
| Msa0080990 | Msa1145020 | 0.800461 | 1.425740e-48 | -8.615850e-47 |
| Msa0080990 | Msa1176160 | 0.818626 | 1.776599e-52 | -8.615850e-47 |
| Msa0080990 | Msa1201160 | 0.811342 | 7.346514e-51 | -8.615850e-47 |
| Msa0080990 | Msa1228190 | 0.874562 | 6.130785e-68 | -8.615850e-47 |
| Msa0080990 | Msa1271260 | 0.848946 | 4.431379e-60 | -8.615850e-47 |
| Msa0080990 | Msa1277400 | 0.810570 | 1.079337e-50 | -8.615850e-47 |
| Msa0080990 | Msa1294290 | 0.815939 | 7.148239e-52 | -8.615850e-47 |
| Msa0080990 | Msa1305310 | 0.803527 | 3.341356e-49 | -8.615850e-47 |
| Msa0080990 | Msa1305510 | 0.801164 | 1.024661e-48 | -8.615850e-47 |
| Msa0080990 | Msa1307150 | 0.803488 | 3.403480e-49 | -8.615850e-47 |
| Msa0080990 | Msa1307190 | 0.823232 | 1.547269e-53 | -8.615850e-47 |
| Msa0080990 | Msa1339580 | 0.961261 | 1.692799e-119 | -8.615850e-47 |
| Msa0080990 | Msa1356990 | 0.971296 | 6.097041e-133 | -8.615850e-47 |
| Msa0080990 | Msa1379970 | 0.852243 | 5.247019e-61 | -8.615850e-47 |
| Msa0080990 | Msa1406780 | 0.833246 | 5.955235e-56 | -8.615850e-47 |
| Msa0080990 | Msa1421550 | 0.841486 | 4.615029e-58 | -8.615850e-47 |
| Msa0080990 | Msa1424690 | 0.810311 | 1.227549e-50 | -8.615850e-47 |
| Msa0080990 | Msa1431850 | 0.842065 | 3.247060e-58 | -8.615850e-47 |
| Msa0080990 | Msa1437700 | 0.815669 | 8.212547e-52 | -8.615850e-47 |
| Msa0080990 | Msa1442630 | 0.811241 | 7.726464e-51 | -8.615850e-47 |
| Msa0080990 | Msa1443140 | 0.824918 | 6.220146e-54 | -8.615850e-47 |
| Msa0080990 | Msa1449940 | 0.808709 | 2.710318e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0080990 | MtrunA17_Chr1g0193271 | 80.723 | 83 | 14 | 1 | 1 | 81 | 1 | 83 | 7.35e-43 | 134 |
| Msa0080990 | MtrunA17_Chr1g0193251 | 83.750 | 80 | 13 | 0 | 2 | 81 | 4 | 83 | 5.68e-36 | 116 |
| Msa0080990 | MtrunA17_Chr7g0271321 | 48.101 | 79 | 38 | 2 | 1 | 76 | 1 | 79 | 4.12e-19 | 73.9 |
| Msa0080990 | MtrunA17_Chr1g0193261 | 56.923 | 65 | 22 | 3 | 15 | 73 | 18 | 82 | 2.23e-17 | 69.7 |
| Msa0080990 | MtrunA17_Chr1g0193231 | 56.923 | 65 | 22 | 3 | 15 | 73 | 18 | 82 | 2.25e-17 | 69.7 |
| Msa0080990 | MtrunA17_Chr1g0193301 | 56.923 | 65 | 22 | 3 | 15 | 73 | 19 | 83 | 2.63e-17 | 69.7 |
| Msa0080990 | MtrunA17_Chr1g0193291 | 59.016 | 61 | 20 | 3 | 18 | 73 | 20 | 80 | 1.01e-15 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0080990 | AT3G05858.1 | 45.882 | 85 | 39 | 4 | 1 | 79 | 1 | 84 | 2.57e-12 | 57.4 |
Find 16 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGATGAAGACAAAGGTAAC+TGG | 0.348340 | 1_2:+59426152 | Msa0080990:CDS |
| GGTAAGACTAAGTCTTCTTC+AGG | 0.365971 | 1_2:+59426043 | Msa0080990:CDS |
| TCAGCTCTCATGTTAATGTT+AGG | 0.378896 | 1_2:-59426187 | None:intergenic |
| AAACTCGATTCTTGTATTTG+CGG | 0.401356 | 1_2:-59426219 | None:intergenic |
| TGGATCAACTTCATAGCGTT+CGG | 0.459202 | 1_2:-59426248 | None:intergenic |
| TTACCTTTGTCTTCATCACT+AGG | 0.502543 | 1_2:-59426148 | None:intergenic |
| TATTATGCATTCTAGACCGC+TGG | 0.510025 | 1_2:-59426268 | None:intergenic |
| TCAAAGAATAAGTCTAGAGC+AGG | 0.540317 | 1_2:+59426094 | Msa0080990:CDS |
| TGATGAAGACAAAGGTAACT+GGG | 0.585582 | 1_2:+59426153 | Msa0080990:CDS |
| GCATTAATCATCATGGGAGG+TGG | 0.588766 | 1_2:+59426022 | None:intergenic |
| GCAGGATACTATGATGATTG+CGG | 0.626514 | 1_2:+59426112 | Msa0080990:CDS |
| ATGATTGCGGATGCAAGACA+TGG | 0.656100 | 1_2:+59426125 | Msa0080990:CDS |
| ACGCTATGAAGTTGATCCAG+CGG | 0.687155 | 1_2:+59426252 | Msa0080990:CDS |
| TGGCCTAGTGATGAAGACAA+AGG | 0.708445 | 1_2:+59426145 | Msa0080990:CDS |
| GCAGCATTAATCATCATGGG+AGG | 0.708531 | 1_2:+59426019 | None:intergenic |
| GATGAAGACAAAGGTAACTG+GGG | 0.719672 | 1_2:+59426154 | Msa0080990:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACTCGATTCTTGTATTTG+CGG | - | chr1_2:59426222-59426241 | None:intergenic | 30.0% | |
| ! | TCAAAGAATAAGTCTAGAGC+AGG | + | chr1_2:59426094-59426113 | Msa0080990:CDS | 35.0% |
| TTACCTTTGTCTTCATCACT+AGG | - | chr1_2:59426151-59426170 | None:intergenic | 35.0% | |
| TGATGAAGACAAAGGTAACT+GGG | + | chr1_2:59426153-59426172 | Msa0080990:CDS | 35.0% | |
| TCAGCTCTCATGTTAATGTT+AGG | - | chr1_2:59426190-59426209 | None:intergenic | 35.0% | |
| GGTAAGACTAAGTCTTCTTC+AGG | + | chr1_2:59426043-59426062 | Msa0080990:CDS | 40.0% | |
| GCAGGATACTATGATGATTG+CGG | + | chr1_2:59426112-59426131 | Msa0080990:CDS | 40.0% | |
| GTGATGAAGACAAAGGTAAC+TGG | + | chr1_2:59426152-59426171 | Msa0080990:CDS | 40.0% | |
| GATGAAGACAAAGGTAACTG+GGG | + | chr1_2:59426154-59426173 | Msa0080990:CDS | 40.0% | |
| TGGATCAACTTCATAGCGTT+CGG | - | chr1_2:59426251-59426270 | None:intergenic | 40.0% | |
| ATGATTGCGGATGCAAGACA+TGG | + | chr1_2:59426125-59426144 | Msa0080990:CDS | 45.0% | |
| TGGCCTAGTGATGAAGACAA+AGG | + | chr1_2:59426145-59426164 | Msa0080990:CDS | 45.0% | |
| ! | ACGCTATGAAGTTGATCCAG+CGG | + | chr1_2:59426252-59426271 | Msa0080990:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 59426034 | 59426279 | 59426034 | ID=Msa0080990;Name=Msa0080990 |
| chr1_2 | mRNA | 59426034 | 59426279 | 59426034 | ID=Msa0080990-mRNA-1;Parent=Msa0080990;Name=Msa0080990-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|81 |
| chr1_2 | exon | 59426034 | 59426279 | 59426034 | ID=Msa0080990-mRNA-1:exon:19242;Parent=Msa0080990-mRNA-1 |
| chr1_2 | CDS | 59426034 | 59426279 | 59426034 | ID=Msa0080990-mRNA-1:cds;Parent=Msa0080990-mRNA-1 |
| Gene Sequence |
| Protein sequence |
