AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0999410


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0999410 MtrunA17_Chr6g0485551 57.500 80 22 1 38 117 42 109 4.56e-20 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 21 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAACCATTTGATCATAAA+AGG 0.244028 6_4:-81358071 None:intergenic
TCGGACTTTCAAGTTCTTTA+CGG 0.318095 6_4:-81358309 None:intergenic
GACAGTCATTTGTGGAAGAT+TGG 0.366580 6_4:-81357821 None:intergenic
ACAGTCATTTGTGGAAGATT+GGG 0.381276 6_4:-81357820 None:intergenic
AGGCTAAAATGTGCGTGGTT+TGG 0.425751 6_4:+81358253 Msa0999410:CDS
CGGAAGCTGAAATTCGAAAG+TGG 0.452981 6_4:+81358386 Msa0999410:CDS
CTCTAAACCAGCAGTTGTTT+AGG 0.457152 6_4:+81358233 Msa0999410:intron
TTCTTCTCGACAGTCATTTG+TGG 0.465053 6_4:-81357829 None:intergenic
TGGTGATTTAAATCAGCATC+CGG 0.494036 6_4:-81358118 None:intergenic
CGAAAGTGGGCAGCTAAAGT+TGG 0.498146 6_4:+81358400 Msa0999410:CDS
AGCTAAAGTTGGAAAGCAGC+AGG 0.505164 6_4:+81358411 Msa0999410:CDS
CAGTCATTTGTGGAAGATTG+GGG 0.526783 6_4:-81357819 None:intergenic
TGGCAAGGCTCATTATAAAG+AGG 0.544099 6_4:+81358273 Msa0999410:CDS
TCGTAGTTGCATGAATAAGC+CGG 0.600958 6_4:+81358099 Msa0999410:CDS
GGATGCTGATTTAAATCACC+AGG 0.601696 6_4:+81358120 Msa0999410:CDS
CATCACATGTTACACAGACA+CGG 0.608031 6_4:+81357279 Msa0999410:CDS
AAAATGTGCGTGGTTTGGCA+AGG 0.609174 6_4:+81358258 Msa0999410:CDS
GGAAGCTGAAATTCGAAAGT+GGG 0.616430 6_4:+81358387 Msa0999410:CDS
CAAGGCTCATTATAAAGAGG+AGG 0.629971 6_4:+81358276 Msa0999410:CDS
TGTTTAGGCTAAAATGTGCG+TGG 0.663685 6_4:+81358248 Msa0999410:CDS
AGTCATTTGTGGAAGATTGG+GGG 0.693821 6_4:-81357818 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTATAGAAAATTAATATTAA+AGG - chr6_4:81358029-81358048 None:intergenic 10.0%
!!! TTCTTTTTAATTTTCTTTTT+CGG - chr6_4:81358331-81358350 None:intergenic 10.0%
!! GTATAGAAAATTAATATTAA+AGG - chr6_4:81358029-81358048 None:intergenic 10.0%
!!! TTCTTTTTAATTTTCTTTTT+CGG - chr6_4:81358331-81358350 None:intergenic 10.0%
!! GAAAAAGAAAATTAAAAAGA+AGG + chr6_4:81358330-81358349 Msa0999410:CDS 15.0%
!! GAAAAAGAAAATTAAAAAGA+AGG + chr6_4:81358330-81358349 Msa0999410:CDS 15.0%
!! TTAATATTAAAGGGCTAAAA+CGG - chr6_4:81358019-81358038 None:intergenic 20.0%
!! GAAAAAGAAAATTAAGAAGA+CGG + chr6_4:81358366-81358385 Msa0999410:CDS 20.0%
!! TTAATATTAAAGGGCTAAAA+CGG - chr6_4:81358019-81358038 None:intergenic 20.0%
!! GAAAAAGAAAATTAAGAAGA+CGG + chr6_4:81358366-81358385 Msa0999410:CDS 20.0%
! GAAATCAATCATAGATTCAA+AGG - chr6_4:81357582-81357601 None:intergenic 25.0%
! GATGATAACAATCATAAAAG+AGG - chr6_4:81357737-81357756 None:intergenic 25.0%
!! TATTAATTTTCTATACCTGC+AGG + chr6_4:81358033-81358052 Msa0999410:intron 25.0%
!! TGATTCCTTTTATGATCAAA+TGG + chr6_4:81358066-81358085 Msa0999410:CDS 25.0%
! ATCAACCATTTGATCATAAA+AGG - chr6_4:81358074-81358093 None:intergenic 25.0%
! GAAATCAATCATAGATTCAA+AGG - chr6_4:81357582-81357601 None:intergenic 25.0%
! GATGATAACAATCATAAAAG+AGG - chr6_4:81357737-81357756 None:intergenic 25.0%
!! TATTAATTTTCTATACCTGC+AGG + chr6_4:81358033-81358052 Msa0999410:intron 25.0%
!! TGATTCCTTTTATGATCAAA+TGG + chr6_4:81358066-81358085 Msa0999410:CDS 25.0%
! ATCAACCATTTGATCATAAA+AGG - chr6_4:81358074-81358093 None:intergenic 25.0%
!!! GAAGATGATGATTTTTTCTG+TGG + chr6_4:81357317-81357336 Msa0999410:CDS 30.0%
!!! ATTTTTTCTGTGGAAGAAAG+TGG + chr6_4:81357327-81357346 Msa0999410:intron 30.0%
TATGATAAACCAATTCAACG+TGG + chr6_4:81357401-81357420 Msa0999410:intron 30.0%
AGGATCAAAGTGATTAAAGT+AGG + chr6_4:81357484-81357503 Msa0999410:intron 30.0%
ATTCGAATATTCTTGCATTC+AGG + chr6_4:81357613-81357632 Msa0999410:intron 30.0%
TTCGAATATTCTTGCATTCA+GGG + chr6_4:81357614-81357633 Msa0999410:intron 30.0%
! ATTTTTGATGAGAAGTTTGC+CGG - chr6_4:81357923-81357942 None:intergenic 30.0%
TCAAAAATGATAGAGATCAG+CGG + chr6_4:81357935-81357954 Msa0999410:intron 30.0%
!! TTCAAGTTCTTTACGGTTTT+CGG - chr6_4:81358305-81358324 None:intergenic 30.0%
!!! GAAGATGATGATTTTTTCTG+TGG + chr6_4:81357317-81357336 Msa0999410:CDS 30.0%
!!! ATTTTTTCTGTGGAAGAAAG+TGG + chr6_4:81357327-81357346 Msa0999410:intron 30.0%
TATGATAAACCAATTCAACG+TGG + chr6_4:81357401-81357420 Msa0999410:intron 30.0%
AGGATCAAAGTGATTAAAGT+AGG + chr6_4:81357484-81357503 Msa0999410:intron 30.0%
ATTCGAATATTCTTGCATTC+AGG + chr6_4:81357613-81357632 Msa0999410:intron 30.0%
TTCGAATATTCTTGCATTCA+GGG + chr6_4:81357614-81357633 Msa0999410:intron 30.0%
! ATTTTTGATGAGAAGTTTGC+CGG - chr6_4:81357923-81357942 None:intergenic 30.0%
TCAAAAATGATAGAGATCAG+CGG + chr6_4:81357935-81357954 Msa0999410:intron 30.0%
!! TTCAAGTTCTTTACGGTTTT+CGG - chr6_4:81358305-81358324 None:intergenic 30.0%
TCGAATATTCTTGCATTCAG+GGG + chr6_4:81357615-81357634 Msa0999410:intron 35.0%
AATGCTTCTTCTCTAGTCTA+GGG + chr6_4:81357662-81357681 Msa0999410:intron 35.0%
ACAGTCATTTGTGGAAGATT+GGG - chr6_4:81357823-81357842 None:intergenic 35.0%
AGAAATCTAAGCCAACTACT+TGG + chr6_4:81357848-81357867 Msa0999410:intron 35.0%
! CTCTTTTTGCCAGTATATGA+GGG - chr6_4:81357886-81357905 None:intergenic 35.0%
! TCTCTTTTTGCCAGTATATG+AGG - chr6_4:81357887-81357906 None:intergenic 35.0%
TATACTGGCAAAAAGAGATC+TGG + chr6_4:81357889-81357908 Msa0999410:intron 35.0%
ATACTGGCAAAAAGAGATCT+GGG + chr6_4:81357890-81357909 Msa0999410:intron 35.0%
CAAAAATGATAGAGATCAGC+GGG + chr6_4:81357936-81357955 Msa0999410:intron 35.0%
TGGTGATTTAAATCAGCATC+CGG - chr6_4:81358121-81358140 None:intergenic 35.0%
TTGTTCGAAAAAAACTTGCC+TGG - chr6_4:81358141-81358160 None:intergenic 35.0%
!! ATTTTAGCCTAAACAACTGC+TGG - chr6_4:81358243-81358262 None:intergenic 35.0%
!! TCGGACTTTCAAGTTCTTTA+CGG - chr6_4:81358312-81358331 None:intergenic 35.0%
TCGAATATTCTTGCATTCAG+GGG + chr6_4:81357615-81357634 Msa0999410:intron 35.0%
AATGCTTCTTCTCTAGTCTA+GGG + chr6_4:81357662-81357681 Msa0999410:intron 35.0%
ACAGTCATTTGTGGAAGATT+GGG - chr6_4:81357823-81357842 None:intergenic 35.0%
AGAAATCTAAGCCAACTACT+TGG + chr6_4:81357848-81357867 Msa0999410:intron 35.0%
! CTCTTTTTGCCAGTATATGA+GGG - chr6_4:81357886-81357905 None:intergenic 35.0%
! TCTCTTTTTGCCAGTATATG+AGG - chr6_4:81357887-81357906 None:intergenic 35.0%
TATACTGGCAAAAAGAGATC+TGG + chr6_4:81357889-81357908 Msa0999410:intron 35.0%
ATACTGGCAAAAAGAGATCT+GGG + chr6_4:81357890-81357909 Msa0999410:intron 35.0%
CAAAAATGATAGAGATCAGC+GGG + chr6_4:81357936-81357955 Msa0999410:intron 35.0%
TGGTGATTTAAATCAGCATC+CGG - chr6_4:81358121-81358140 None:intergenic 35.0%
TTGTTCGAAAAAAACTTGCC+TGG - chr6_4:81358141-81358160 None:intergenic 35.0%
!! ATTTTAGCCTAAACAACTGC+TGG - chr6_4:81358243-81358262 None:intergenic 35.0%
!! TCGGACTTTCAAGTTCTTTA+CGG - chr6_4:81358312-81358331 None:intergenic 35.0%
CATCACATGTTACACAGACA+CGG + chr6_4:81357279-81357298 Msa0999410:CDS 40.0%
! AGTCTGTTGCTGCAAGTTTT+GGG - chr6_4:81357365-81357384 None:intergenic 40.0%
! TAGTCTGTTGCTGCAAGTTT+TGG - chr6_4:81357366-81357385 None:intergenic 40.0%
ACTTGCAGCAACAGACTAAT+TGG + chr6_4:81357368-81357387 Msa0999410:intron 40.0%
CTTGCAGCAACAGACTAATT+GGG + chr6_4:81357369-81357388 Msa0999410:intron 40.0%
TGCAAAGATCCACGTTGAAT+TGG - chr6_4:81357413-81357432 None:intergenic 40.0%
! CTTTGGGAAAATTGTCACAG+AGG + chr6_4:81357464-81357483 Msa0999410:intron 40.0%
ATTCTTGCATTCAGGGGTTT+AGG + chr6_4:81357621-81357640 Msa0999410:intron 40.0%
TTCTTGCATTCAGGGGTTTA+GGG + chr6_4:81357622-81357641 Msa0999410:intron 40.0%
CAATGCTTCTTCTCTAGTCT+AGG + chr6_4:81357661-81357680 Msa0999410:intron 40.0%
AGTCATTTGTGGAAGATTGG+GGG - chr6_4:81357821-81357840 None:intergenic 40.0%
CAGTCATTTGTGGAAGATTG+GGG - chr6_4:81357822-81357841 None:intergenic 40.0%
GACAGTCATTTGTGGAAGAT+TGG - chr6_4:81357824-81357843 None:intergenic 40.0%
TTCTTCTCGACAGTCATTTG+TGG - chr6_4:81357832-81357851 None:intergenic 40.0%
!!! ATGAGAAGTTTGCCGGTTTT+TGG - chr6_4:81357916-81357935 None:intergenic 40.0%
TCGTAGTTGCATGAATAAGC+CGG + chr6_4:81358099-81358118 Msa0999410:CDS 40.0%
!! GGATGCTGATTTAAATCACC+AGG + chr6_4:81358120-81358139 Msa0999410:CDS 40.0%
!! CTAAACAACTGCTGGTTTAG+AGG - chr6_4:81358235-81358254 None:intergenic 40.0%
CTCTAAACCAGCAGTTGTTT+AGG + chr6_4:81358233-81358252 Msa0999410:intron 40.0%
TGTTTAGGCTAAAATGTGCG+TGG + chr6_4:81358248-81358267 Msa0999410:CDS 40.0%
TGGCAAGGCTCATTATAAAG+AGG + chr6_4:81358273-81358292 Msa0999410:CDS 40.0%
CAAGGCTCATTATAAAGAGG+AGG + chr6_4:81358276-81358295 Msa0999410:CDS 40.0%
GGAAGCTGAAATTCGAAAGT+GGG + chr6_4:81358387-81358406 Msa0999410:CDS 40.0%
CATCACATGTTACACAGACA+CGG + chr6_4:81357279-81357298 Msa0999410:CDS 40.0%
! AGTCTGTTGCTGCAAGTTTT+GGG - chr6_4:81357365-81357384 None:intergenic 40.0%
! TAGTCTGTTGCTGCAAGTTT+TGG - chr6_4:81357366-81357385 None:intergenic 40.0%
ACTTGCAGCAACAGACTAAT+TGG + chr6_4:81357368-81357387 Msa0999410:intron 40.0%
CTTGCAGCAACAGACTAATT+GGG + chr6_4:81357369-81357388 Msa0999410:intron 40.0%
TGCAAAGATCCACGTTGAAT+TGG - chr6_4:81357413-81357432 None:intergenic 40.0%
! CTTTGGGAAAATTGTCACAG+AGG + chr6_4:81357464-81357483 Msa0999410:intron 40.0%
ATTCTTGCATTCAGGGGTTT+AGG + chr6_4:81357621-81357640 Msa0999410:intron 40.0%
TTCTTGCATTCAGGGGTTTA+GGG + chr6_4:81357622-81357641 Msa0999410:intron 40.0%
CAATGCTTCTTCTCTAGTCT+AGG + chr6_4:81357661-81357680 Msa0999410:intron 40.0%
AGTCATTTGTGGAAGATTGG+GGG - chr6_4:81357821-81357840 None:intergenic 40.0%
CAGTCATTTGTGGAAGATTG+GGG - chr6_4:81357822-81357841 None:intergenic 40.0%
GACAGTCATTTGTGGAAGAT+TGG - chr6_4:81357824-81357843 None:intergenic 40.0%
TTCTTCTCGACAGTCATTTG+TGG - chr6_4:81357832-81357851 None:intergenic 40.0%
!!! ATGAGAAGTTTGCCGGTTTT+TGG - chr6_4:81357916-81357935 None:intergenic 40.0%
TCGTAGTTGCATGAATAAGC+CGG + chr6_4:81358099-81358118 Msa0999410:CDS 40.0%
!! GGATGCTGATTTAAATCACC+AGG + chr6_4:81358120-81358139 Msa0999410:CDS 40.0%
!! CTAAACAACTGCTGGTTTAG+AGG - chr6_4:81358235-81358254 None:intergenic 40.0%
CTCTAAACCAGCAGTTGTTT+AGG + chr6_4:81358233-81358252 Msa0999410:intron 40.0%
TGTTTAGGCTAAAATGTGCG+TGG + chr6_4:81358248-81358267 Msa0999410:CDS 40.0%
TGGCAAGGCTCATTATAAAG+AGG + chr6_4:81358273-81358292 Msa0999410:CDS 40.0%
CAAGGCTCATTATAAAGAGG+AGG + chr6_4:81358276-81358295 Msa0999410:CDS 40.0%
GGAAGCTGAAATTCGAAAGT+GGG + chr6_4:81358387-81358406 Msa0999410:CDS 40.0%
! GACACGGTTTTCGAATACTG+AGG + chr6_4:81357295-81357314 Msa0999410:CDS 45.0%
TTCAACGTGGATCTTTGCAG+AGG + chr6_4:81357414-81357433 Msa0999410:intron 45.0%
TCAACGTGGATCTTTGCAGA+GGG + chr6_4:81357415-81357434 Msa0999410:intron 45.0%
! TTTTCCCAAAGCGAGATGCT+GGG - chr6_4:81357455-81357474 None:intergenic 45.0%
! ATTTTCCCAAAGCGAGATGC+TGG - chr6_4:81357456-81357475 None:intergenic 45.0%
AAGAGATCTGGGCCAAAAAC+CGG + chr6_4:81357901-81357920 Msa0999410:intron 45.0%
AATCAAACAACGAAGCCTGC+AGG - chr6_4:81358051-81358070 None:intergenic 45.0%
AGGCTAAAATGTGCGTGGTT+TGG + chr6_4:81358253-81358272 Msa0999410:CDS 45.0%
! AAAATGTGCGTGGTTTGGCA+AGG + chr6_4:81358258-81358277 Msa0999410:CDS 45.0%
CGGAAGCTGAAATTCGAAAG+TGG + chr6_4:81358386-81358405 Msa0999410:CDS 45.0%
AGCTAAAGTTGGAAAGCAGC+AGG + chr6_4:81358411-81358430 Msa0999410:CDS 45.0%
! GACACGGTTTTCGAATACTG+AGG + chr6_4:81357295-81357314 Msa0999410:CDS 45.0%
TTCAACGTGGATCTTTGCAG+AGG + chr6_4:81357414-81357433 Msa0999410:intron 45.0%
TCAACGTGGATCTTTGCAGA+GGG + chr6_4:81357415-81357434 Msa0999410:intron 45.0%
! TTTTCCCAAAGCGAGATGCT+GGG - chr6_4:81357455-81357474 None:intergenic 45.0%
! ATTTTCCCAAAGCGAGATGC+TGG - chr6_4:81357456-81357475 None:intergenic 45.0%
AAGAGATCTGGGCCAAAAAC+CGG + chr6_4:81357901-81357920 Msa0999410:intron 45.0%
AATCAAACAACGAAGCCTGC+AGG - chr6_4:81358051-81358070 None:intergenic 45.0%
AGGCTAAAATGTGCGTGGTT+TGG + chr6_4:81358253-81358272 Msa0999410:CDS 45.0%
! AAAATGTGCGTGGTTTGGCA+AGG + chr6_4:81358258-81358277 Msa0999410:CDS 45.0%
CGGAAGCTGAAATTCGAAAG+TGG + chr6_4:81358386-81358405 Msa0999410:CDS 45.0%
AGCTAAAGTTGGAAAGCAGC+AGG + chr6_4:81358411-81358430 Msa0999410:CDS 45.0%
!! TATAGAAAATTAATATTAAA+GGG - chr6_4:81358028-81358047 None:intergenic 5.0%
!! TATAGAAAATTAATATTAAA+GGG - chr6_4:81358028-81358047 None:intergenic 5.0%
AGCGAGATGCTGGGACTAAT+TGG - chr6_4:81357446-81357465 None:intergenic 50.0%
TTAGTCCCAGCATCTCGCTT+TGG + chr6_4:81357447-81357466 Msa0999410:intron 50.0%
TAGTCCCAGCATCTCGCTTT+GGG + chr6_4:81357448-81357467 Msa0999410:intron 50.0%
CCTGCGATGATCCAAGTAGT+TGG - chr6_4:81357862-81357881 None:intergenic 50.0%
CCAACTACTTGGATCATCGC+AGG + chr6_4:81357859-81357878 Msa0999410:intron 50.0%
ATCGCAGGACCCTCATATAC+TGG + chr6_4:81357874-81357893 Msa0999410:intron 50.0%
CGAAAGTGGGCAGCTAAAGT+TGG + chr6_4:81358400-81358419 Msa0999410:CDS 50.0%
AGCGAGATGCTGGGACTAAT+TGG - chr6_4:81357446-81357465 None:intergenic 50.0%
TTAGTCCCAGCATCTCGCTT+TGG + chr6_4:81357447-81357466 Msa0999410:intron 50.0%
TAGTCCCAGCATCTCGCTTT+GGG + chr6_4:81357448-81357467 Msa0999410:intron 50.0%
CCTGCGATGATCCAAGTAGT+TGG - chr6_4:81357862-81357881 None:intergenic 50.0%
CCAACTACTTGGATCATCGC+AGG + chr6_4:81357859-81357878 Msa0999410:intron 50.0%
ATCGCAGGACCCTCATATAC+TGG + chr6_4:81357874-81357893 Msa0999410:intron 50.0%
CGAAAGTGGGCAGCTAAAGT+TGG + chr6_4:81358400-81358419 Msa0999410:CDS 50.0%
GGGAGAGAGTTAAGCTGCAG+AGG - chr6_4:81357801-81357820 None:intergenic 55.0%
GGGAGAGAGTTAAGCTGCAG+AGG - chr6_4:81357801-81357820 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 81357272 81358441 81357272 ID=Msa0999410;Name=Msa0999410
chr6_4 mRNA 81357272 81358441 81357272 ID=Msa0999410-mRNA-1;Parent=Msa0999410;Name=Msa0999410-mRNA-1;_AED=0.37;_eAED=0.42;_QI=0|0|0|1|0|0|4|0|142
chr6_4 exon 81357272 81357345 81357272 ID=Msa0999410-mRNA-1:exon:15544;Parent=Msa0999410-mRNA-1
chr6_4 exon 81357807 81357840 81357807 ID=Msa0999410-mRNA-1:exon:15545;Parent=Msa0999410-mRNA-1
chr6_4 exon 81358055 81358174 81358055 ID=Msa0999410-mRNA-1:exon:15546;Parent=Msa0999410-mRNA-1
chr6_4 exon 81358244 81358441 81358244 ID=Msa0999410-mRNA-1:exon:15547;Parent=Msa0999410-mRNA-1
chr6_4 CDS 81357272 81357345 81357272 ID=Msa0999410-mRNA-1:cds;Parent=Msa0999410-mRNA-1
chr6_4 CDS 81357807 81357840 81357807 ID=Msa0999410-mRNA-1:cds;Parent=Msa0999410-mRNA-1
chr6_4 CDS 81358055 81358174 81358055 ID=Msa0999410-mRNA-1:cds;Parent=Msa0999410-mRNA-1
chr6_4 CDS 81358244 81358441 81358244 ID=Msa0999410-mRNA-1:cds;Parent=Msa0999410-mRNA-1
Gene Sequence

>Msa0999410

ATGAAAACATCACATGTTACACAGACACGGTTTTCGAATACTGAGGAAGATGATGATTTTTTCTGTGGAAGAAACTTAACTCTCTCCCCCAATCTTCCACAAATGACTGCTTCGTTGTTTGATTCCTTTTATGATCAAATGGTTGATGATGTTCGTAGTTGCATGAATAAGCCGGATGCTGATTTAAATCACCAGGCAAGTTTTTTTCGAACAATGTTTTTGATTCAACAGTTGTTTAGGCTAAAATGTGCGTGGTTTGGCAAGGCTCATTATAAAGAGGAGGTTGCCGAAAACCGTAAAGAACTTGAAAGTCCGAAAAAGAAAATTAAAAAGAAGGAAGCTGAAATTCTGAAAAAGAAAATTAAGAAGACGGAAGCTGAAATTCGAAAGTGGGCAGCTAAAGTTGGAAAGCAGCAGGAGAAGAAA

Protein sequence

>Msa0999410

MKTSHVTQTRFSNTEEDDDFFCGRNLTLSPNLPQMTASLFDSFYDQMVDDVRSCMNKPDADLNHQASFFRTMFLIQQLFRLKCAWFGKAHYKEEVAENRKELESPKKKIKKKEAEILKKKIKKTEAEIRKWAAKVGKQQEKK