AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1379970


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCCTTTGGTACGGTAAAA+TGG 0.241786 tig0020315:+124827 Msa1379970:CDS
CAATGGTTGTGGGGTTCCTT+TGG 0.325217 tig0020315:+124814 None:intergenic
AGGGCAAAAGCATAAAATAT+TGG 0.368692 tig0020315:-125139 None:intergenic
GTCCATCTTCTGCTGTATCT+CGG 0.388400 tig0020315:+125045 Msa1379970:CDS
TCCATCTTCTGCTGTATCTC+GGG 0.445994 tig0020315:+125046 Msa1379970:CDS
TATTAATTAACAATGGTTGT+GGG 0.467711 tig0020315:+124804 None:intergenic
GTATTAATTAACAATGGTTG+TGG 0.474200 tig0020315:+124803 None:intergenic
ACGGTAAAATGGATTCCAAC+AGG 0.494468 tig0020315:+124838 Msa1379970:CDS
GGAGACATCGATTCAAGTCT+AGG 0.508656 tig0020315:-125097 None:intergenic
GCCCGAGATACAGCAGAAGA+TGG 0.518961 tig0020315:-125047 None:intergenic
GTTGTGGGGTTCCTTTGGTA+CGG 0.531669 tig0020315:+124819 Msa1379970:CDS
ATTAATTAACAATGGTTGTG+GGG 0.582885 tig0020315:+124805 None:intergenic
TAAAATATTGGAAACTGCAG+TGG 0.596637 tig0020315:-125127 None:intergenic
AAGTATGCAAGATCATGAGC+AGG 0.597944 tig0020315:+125018 Msa1379970:intron
TGGAAACTGCAGTGGTGCTG+TGG 0.605136 tig0020315:-125119 None:intergenic
GGAAACTGCAGTGGTGCTGT+GGG 0.653534 tig0020315:-125118 None:intergenic
GTTGCAGCTGAGAAAAGACG+AGG 0.732163 tig0020315:-125069 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAAAAATTACAAAATTAAT+CGG - tig0020315:125233-125252 None:intergenic 10.0%
!!! ATAGTTTTCCATTTAGAGTT+AGG + tig0020315:124878-124897 Msa1379970:intron 25.0%
!! TACGAATTTGCATTTTACAA+GGG - tig0020315:125161-125180 None:intergenic 25.0%
!! ATACGAATTTGCATTTTACA+AGG - tig0020315:125162-125181 None:intergenic 25.0%
! TTCCATTTAGAGTTAGGTTA+AGG + tig0020315:124884-124903 Msa1379970:intron 30.0%
TACCTTAACCTAACTCTAAA+TGG - tig0020315:124889-124908 None:intergenic 30.0%
TTCCAATTAGAGTTAGGTTA+AGG + tig0020315:124936-124955 Msa1379970:intron 30.0%
AACCTTAACCTAACTCTAAT+TGG - tig0020315:124941-124960 None:intergenic 30.0%
TAAAATATTGGAAACTGCAG+TGG - tig0020315:125130-125149 None:intergenic 30.0%
! AGGGCAAAAGCATAAAATAT+TGG - tig0020315:125142-125161 None:intergenic 30.0%
! AATCCATTTTACCGTACCAA+AGG - tig0020315:124833-124852 None:intergenic 35.0%
AACTCTAATTGGAAGATCGA+GGG - tig0020315:124930-124949 None:intergenic 35.0%
TAACTCTAATTGGAAGATCG+AGG - tig0020315:124931-124950 None:intergenic 35.0%
TCGATCTTCCAATTAGAGTT+AGG + tig0020315:124930-124949 Msa1379970:intron 35.0%
GTTCCTTTGGTACGGTAAAA+TGG + tig0020315:124827-124846 Msa1379970:CDS 40.0%
! ACGGTAAAATGGATTCCAAC+AGG + tig0020315:124838-124857 Msa1379970:CDS 40.0%
AAGTATGCAAGATCATGAGC+AGG + tig0020315:125018-125037 Msa1379970:intron 40.0%
AGTATCATGTGCGAACCTGT+TGG - tig0020315:124856-124875 None:intergenic 45.0%
GTCCATCTTCTGCTGTATCT+CGG + tig0020315:125045-125064 Msa1379970:CDS 45.0%
TCCATCTTCTGCTGTATCTC+GGG + tig0020315:125046-125065 Msa1379970:CDS 45.0%
!! GGAGACATCGATTCAAGTCT+AGG - tig0020315:125100-125119 None:intergenic 45.0%
GTTGTGGGGTTCCTTTGGTA+CGG + tig0020315:124819-124838 Msa1379970:CDS 50.0%
GTTGCAGCTGAGAAAAGACG+AGG - tig0020315:125072-125091 None:intergenic 50.0%
GCCCGAGATACAGCAGAAGA+TGG - tig0020315:125050-125069 None:intergenic 55.0%
!! GGAAACTGCAGTGGTGCTGT+GGG - tig0020315:125121-125140 None:intergenic 55.0%
! TGGAAACTGCAGTGGTGCTG+TGG - tig0020315:125122-125141 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0020315 gene 124816 125265 124816 ID=Msa1379970;Name=Msa1379970
tig0020315 mRNA 124816 125265 124816 ID=Msa1379970-mRNA-1;Parent=Msa1379970;Name=Msa1379970-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|0|0|0.25|1|1|4|0|65
tig0020315 exon 124816 124859 124816 ID=Msa1379970-mRNA-1:exon:6846;Parent=Msa1379970-mRNA-1
tig0020315 exon 124900 124905 124900 ID=Msa1379970-mRNA-1:exon:6847;Parent=Msa1379970-mRNA-1
tig0020315 exon 125021 125162 125021 ID=Msa1379970-mRNA-1:exon:6848;Parent=Msa1379970-mRNA-1
tig0020315 exon 125260 125265 125260 ID=Msa1379970-mRNA-1:exon:6849;Parent=Msa1379970-mRNA-1
tig0020315 CDS 124816 124859 124816 ID=Msa1379970-mRNA-1:cds;Parent=Msa1379970-mRNA-1
tig0020315 CDS 124900 124905 124900 ID=Msa1379970-mRNA-1:cds;Parent=Msa1379970-mRNA-1
tig0020315 CDS 125021 125162 125021 ID=Msa1379970-mRNA-1:cds;Parent=Msa1379970-mRNA-1
tig0020315 CDS 125260 125265 125260 ID=Msa1379970-mRNA-1:cds;Parent=Msa1379970-mRNA-1
Gene Sequence

>Msa1379970

ATGGTTGTGGGGTTCCTTTGGTACGGTAAAATGGATTCCAACAGGTTAAGTATGCAAGATCATGAGCAGGTCGAGTCCATCTTCTGCTGTATCTCGGGCCTCGTCTTTTCTCAGCTGCAACAAGTTCCTAGACTTGAATCGATGTCTCCCACAGCACCACTGCAGTTTCCAATATTTTATGCTTTTGCCCTTATTTAA

Protein sequence

>Msa1379970

MVVGFLWYGKMDSNRLSMQDHEQVESIFCCISGLVFSQLQQVPRLESMSPTAPLQFPIFYAFALI*