AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0550420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0550420 MtrunA17_Chr4g0035271 100.000 107 0 0 43 149 1 107 1.94e-74 217
Msa0550420 MtrunA17_Chr2g0321571 99.065 107 1 0 43 149 1 107 4.33e-74 216
Msa0550420 MtrunA17_Chr6g0455051 75.556 45 2 1 68 112 6 41 2.75e-16 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0550420 AT2G21580.1 92.593 108 7 1 43 149 1 108 3.29e-53 164
Msa0550420 AT4G39200.1 89.815 108 10 1 43 149 1 108 1.12e-51 160
Msa0550420 AT2G21580.2 88.889 108 10 2 43 149 1 107 1.51e-51 159
Msa0550420 AT4G39200.2 87.963 108 11 2 43 149 1 107 5.12e-50 155
Msa0550420 AT4G34555.1 89.720 107 10 1 43 148 1 107 1.17e-49 155
Msa0550420 AT2G16360.1 86.667 105 13 1 44 147 2 106 4.54e-46 146

Find 58 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTGGTCTATGCAGATTAA+TGG 0.196241 4_1:-45838075 Msa0550420:intron
AGGAGTGATGAGCTTGTATT+TGG 0.258319 4_1:+45839326 None:intergenic
CTGTTTGCCACCGCCAGATT+TGG 0.259942 4_1:+45840242 None:intergenic
TTCTTGAAGGATATGGGTTT+TGG 0.267085 4_1:-45841231 None:intergenic
GGAGGAGGAGCTGCCTTCTT+GGG 0.308666 4_1:+45840279 None:intergenic
TCCTCCAAGCCAGCCAAATC+TGG 0.317280 4_1:-45840255 Msa0550420:CDS
ACCGCCAGATTTGGCTGGCT+TGG 0.348344 4_1:+45840251 None:intergenic
GCAATTCAACTAAATAAAAT+AGG 0.360931 4_1:+45837773 None:intergenic
AGGAGGAGGAGCTGCCTTCT+TGG 0.379715 4_1:+45840278 None:intergenic
TGATGGCTAAAGGTTTAATC+AGG 0.382324 4_1:-45838023 Msa0550420:CDS
CTGTCCTCTCTGATCGTTTG+AGG 0.389732 4_1:-45839302 Msa0550420:CDS
GAGGGCTATAAGAGACTTGA+TGG 0.404889 4_1:-45838040 Msa0550420:CDS
CACCTAGGTGTTTGTAGCCC+TGG 0.406263 4_1:+45837966 None:intergenic
TTGCCACCGCCAGATTTGGC+TGG 0.423311 4_1:+45840246 None:intergenic
CAACATGGTGCTGTTTGATC+AGG 0.424061 4_1:-45839379 Msa0550420:CDS
AATCCATACCAATTGGCAAT+TGG 0.425398 4_1:-45837726 Msa0550420:three_prime_UTR
AAACCAATTGCCAATTGGTA+TGG 0.451391 4_1:+45837723 None:intergenic
TGTCCTCTCTGATCGTTTGA+GGG 0.452861 4_1:-45839301 Msa0550420:intron
GCCAGATTTGGCTGGCTTGG+AGG 0.465517 4_1:+45840254 None:intergenic
ATAAGAGACTTGATGGCTAA+AGG 0.477465 4_1:-45838033 Msa0550420:CDS
ATCAGAAACCAATTGCCAAT+TGG 0.479027 4_1:+45837718 None:intergenic
GTGGAGCAAGGGAAAGCAAA+AGG 0.500179 4_1:-45839412 Msa0550420:CDS
GCTGGCTTGGAGGAAGGAGG+AGG 0.501135 4_1:+45840264 None:intergenic
TTCAGTTAAGAGCTTGTCAT+AGG 0.503535 4_1:+45839353 None:intergenic
GGTTCATGATTTGGTCAGGT+GGG 0.509474 4_1:-45837676 Msa0550420:three_prime_UTR
TGGTTCATGATTTGGTCAGG+TGG 0.515484 4_1:-45837677 Msa0550420:three_prime_UTR
GGCTAAAGGTTTAATCAGGT+TGG 0.524410 4_1:-45838019 Msa0550420:CDS
TAATGGATCACTTGCAAGGA+GGG 0.537201 4_1:-45838058 Msa0550420:CDS
GATTTGGCTGGCTTGGAGGA+AGG 0.542704 4_1:+45840258 None:intergenic
ATACCTCGGAGCAGAAATCA+TGG 0.547670 4_1:-45840386 Msa0550420:intron
TCTCAGACCTTCTCATACCT+CGG 0.548806 4_1:-45840400 Msa0550420:CDS
ATAAACTCCTGTGTGAATTG+TGG 0.553497 4_1:-45837573 Msa0550420:three_prime_UTR
CAAGTCAGCAGATTTACACC+AGG 0.557612 4_1:-45837984 Msa0550420:CDS
TGTTTAACAGAAGTGGAGCA+AGG 0.569376 4_1:-45839424 Msa0550420:intron
AAGCCAGCCAAATCTGGCGG+TGG 0.569616 4_1:-45840249 Msa0550420:CDS
GGAGTGATGAGCTTGTATTT+GGG 0.571965 4_1:+45839327 None:intergenic
TATGAGAAGGTCTGAGACGG+AGG 0.575184 4_1:+45840406 None:intergenic
AAACGATCAGAGAGGACAGA+AGG 0.576753 4_1:+45839306 None:intergenic
AATTGGCAATTGGTTTCTGA+TGG 0.585066 4_1:-45837716 Msa0550420:three_prime_UTR
AAGTTGGAGAGTATCAGTAG+AGG 0.586875 4_1:-45837930 Msa0550420:three_prime_UTR
TTGGCTGGCTTGGAGGAAGG+AGG 0.587842 4_1:+45840261 None:intergenic
CGGTGGCAAACAGAAGAAGA+AGG 0.599544 4_1:-45840232 Msa0550420:intron
TTCTGCTCCGAGGTATGAGA+AGG 0.615505 4_1:+45840393 None:intergenic
GTTGACGGAAGCTGACGGCA+AGG 0.615842 4_1:-45840981 Msa0550420:CDS
GTTTAACAGAAGTGGAGCAA+GGG 0.616121 4_1:-45839423 Msa0550420:intron
AACATGGTGCTGTTTGATCA+GGG 0.617310 4_1:-45839378 Msa0550420:CDS
GTGTGCTCCGTTAGCCACCA+CGG 0.625681 4_1:-45841011 Msa0550420:intron
TCCAAGCCAGCCAAATCTGG+CGG 0.632166 4_1:-45840252 Msa0550420:CDS
AGATTAATGGATCACTTGCA+AGG 0.633639 4_1:-45838062 Msa0550420:CDS
AGGTATGAGAAGGTCTGAGA+CGG 0.638023 4_1:+45840403 None:intergenic
TTAAAATCAGTATCACACCT+AGG 0.676138 4_1:+45837951 None:intergenic
AAGTCAGCAGATTTACACCA+GGG 0.688410 4_1:-45837983 Msa0550420:CDS
TTAATGGATCACTTGCAAGG+AGG 0.700095 4_1:-45838059 Msa0550420:CDS
CACCAGGGCTACAAACACCT+AGG 0.701247 4_1:-45837968 Msa0550420:exon
GGACGTTCCACAATTCACAC+AGG 0.710811 4_1:+45837566 None:intergenic
ACACCATGATTTCTGCTCCG+AGG 0.726627 4_1:+45840383 None:intergenic
AAAGGAGAAAGTGAACAACA+TGG 0.737054 4_1:-45839394 Msa0550420:CDS
TTACCCTCAAACGATCAGAG+AGG 0.790654 4_1:+45839298 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATGATAAAAAAATTCAA+CGG - chr4_1:45838086-45838105 Msa0550420:intron 10.0%
!!! TTTAATTTTTAAATTAGCAT+TGG + chr4_1:45839284-45839303 None:intergenic 10.0%
!!! ATTATTGATGAATTAAATTT+TGG + chr4_1:45840683-45840702 None:intergenic 10.0%
!!! TTATTTTTATATCGTTTATT+TGG - chr4_1:45840960-45840979 Msa0550420:intron 10.0%
!! TGATAAGTTGTTAAAATATA+GGG + chr4_1:45837719-45837738 None:intergenic 15.0%
!! ATGATAAGTTGTTAAAATAT+AGG + chr4_1:45837720-45837739 None:intergenic 15.0%
!! ATAAACAATTAAATCTAAAC+AGG + chr4_1:45838701-45838720 None:intergenic 15.0%
!!! TTTTGTATAATCTATTTCTA+AGG + chr4_1:45839139-45839158 None:intergenic 15.0%
!!! TGAACACTTTTTTTTTTTTA+TGG - chr4_1:45841162-45841181 Msa0550420:intron 15.0%
!! TAATGGCTTAAATATGAAAA+AGG + chr4_1:45837926-45837945 None:intergenic 20.0%
!! CACTAGTAATAGTAATATAA+TGG + chr4_1:45837943-45837962 None:intergenic 20.0%
!!! TATCATTTTCATGTGTTTTT+AGG + chr4_1:45838075-45838094 None:intergenic 20.0%
!! TAAGTACAAGAAAAGTTAAA+AGG + chr4_1:45838594-45838613 None:intergenic 20.0%
!!! AATTTAATTTTTGGCCTTTT+TGG - chr4_1:45838822-45838841 Msa0550420:intron 20.0%
!! AAAAAAAAAACTTTACCACA+CGG + chr4_1:45839189-45839208 None:intergenic 20.0%
!!! TGATTTGTTAGTGATATTAA+GGG - chr4_1:45839332-45839351 Msa0550420:CDS 20.0%
!! AAACTAGTAATGAAAATCAT+AGG + chr4_1:45839836-45839855 None:intergenic 20.0%
!!! AAGATAATAACTGCAATTTT+CGG + chr4_1:45840262-45840281 None:intergenic 20.0%
!!! ACACAAATTTAAGTTGATAA+TGG - chr4_1:45840317-45840336 Msa0550420:intron 20.0%
!! ACTAAATATTTGTGACTAAA+AGG - chr4_1:45840644-45840663 Msa0550420:intron 20.0%
!! CTAAATATTTGTGACTAAAA+GGG - chr4_1:45840645-45840664 Msa0550420:intron 20.0%
!!! AATAATGAATATGTCTTTTG+TGG - chr4_1:45840697-45840716 Msa0550420:intron 20.0%
!! AACGATATAAAAATAATCTC+AGG + chr4_1:45840956-45840975 None:intergenic 20.0%
!! GCAATTCAACTAAATAAAAT+AGG + chr4_1:45841019-45841038 None:intergenic 20.0%
!! ATCTTTCTTCTAAGAATTCT+AGG - chr4_1:45838329-45838348 Msa0550420:intron 25.0%
!! TCTTTCTTCTAAGAATTCTA+GGG - chr4_1:45838330-45838349 Msa0550420:intron 25.0%
! TACAAAGAGATGAAATTCAA+CGG + chr4_1:45838448-45838467 None:intergenic 25.0%
! ATGTTAAAAGCTTATGTAAG+TGG + chr4_1:45838724-45838743 None:intergenic 25.0%
! AAAAAGCTGTTAATCCAAAA+AGG + chr4_1:45838839-45838858 None:intergenic 25.0%
!!! AGTTGTTTTGTAAGCTATAT+TGG - chr4_1:45838974-45838993 Msa0550420:intron 25.0%
!! GAAAACAACGAATTTTCAAT+CGG + chr4_1:45839262-45839281 None:intergenic 25.0%
!!! GTGATTTGTTAGTGATATTA+AGG - chr4_1:45839331-45839350 Msa0550420:CDS 25.0%
!!! TTTTTTGTGTTTAACAGAAG+TGG - chr4_1:45839358-45839377 Msa0550420:CDS 25.0%
!! TTTACTAATGCCTTTTCTTA+TGG - chr4_1:45839518-45839537 Msa0550420:intron 25.0%
!!! AAATTTTTCAGTCCTAGTAT+TGG - chr4_1:45839727-45839746 Msa0550420:intron 25.0%
! CTAAATCACAGAACATATAT+TGG - chr4_1:45839950-45839969 Msa0550420:intron 25.0%
! AGAAGATGAAATGTTAACTA+GGG - chr4_1:45840183-45840202 Msa0550420:intron 25.0%
! TCTTGAAGATTCTATTGTTT+AGG - chr4_1:45840278-45840297 Msa0550420:CDS 25.0%
! AATGTCATCATAAAGTAAAG+AGG - chr4_1:45840342-45840361 Msa0550420:intron 25.0%
! AATGACAACGATGAAATTTA+AGG + chr4_1:45840368-45840387 None:intergenic 25.0%
! AAATGAACTTAAAATGCACA+AGG + chr4_1:45840431-45840450 None:intergenic 25.0%
!!! GTTCATTTTTTTGCAATCTT+AGG - chr4_1:45840443-45840462 Msa0550420:intron 25.0%
! CTTACACCAAATAATACATT+AGG + chr4_1:45840506-45840525 None:intergenic 25.0%
! CTTTAGTTGTAAAAATGTTG+TGG - chr4_1:45840533-45840552 Msa0550420:intron 25.0%
!!! TTGTATTAGTTTTGCTGAAT+CGG - chr4_1:45840927-45840946 Msa0550420:intron 25.0%
!!! TAGTTGAATTGCTTTTGTAA+TGG - chr4_1:45841027-45841046 Msa0550420:intron 25.0%
!!! ATTTTGAAATCCATACCAAT+TGG - chr4_1:45841056-45841075 Msa0550420:intron 25.0%
!!! TTAGTTTTTGGTTCATGATT+TGG - chr4_1:45841104-45841123 Msa0550420:intron 25.0%
CAAAAACGTAAATTGACACA+GGG + chr4_1:45837688-45837707 None:intergenic 30.0%
TCAAAAACGTAAATTGACAC+AGG + chr4_1:45837689-45837708 None:intergenic 30.0%
!! TTTTGCTAACCTTTTGGAAA+AGG + chr4_1:45837835-45837854 None:intergenic 30.0%
CAAAAGGTTAGCAAAATACA+GGG - chr4_1:45837839-45837858 Msa0550420:three_prime_UTR 30.0%
TTCGCAACAAAAAAACTTAC+AGG - chr4_1:45837867-45837886 Msa0550420:three_prime_UTR 30.0%
TCGCAACAAAAAAACTTACA+GGG - chr4_1:45837868-45837887 Msa0550420:three_prime_UTR 30.0%
! TACACTTTTAAATGTGAAGC+TGG + chr4_1:45837979-45837998 None:intergenic 30.0%
CTGACTTATAACTAGAAAAC+CGG + chr4_1:45838125-45838144 None:intergenic 30.0%
!!! GTTTTCTAGTTATAAGTCAG+TGG - chr4_1:45838125-45838144 Msa0550420:intron 30.0%
!! TTTTGTTTCCATGTGTTTCA+AGG - chr4_1:45838172-45838191 Msa0550420:intron 30.0%
ATGAATTACAGAGATGAGTA+TGG + chr4_1:45838478-45838497 None:intergenic 30.0%
!!! CTCTGTAATTCATTTTTTGC+AGG - chr4_1:45838485-45838504 Msa0550420:intron 30.0%
TCTAACAAAACTTATGCAAG+TGG - chr4_1:45839070-45839089 Msa0550420:intron 30.0%
TTATACCACACCATAAGAAA+AGG + chr4_1:45839531-45839550 None:intergenic 30.0%
CTAGGACTGAAAAATTTAGA+TGG + chr4_1:45839724-45839743 None:intergenic 30.0%
AAACTAATTCCACCAATACT+AGG + chr4_1:45839742-45839761 None:intergenic 30.0%
AAATCATAGGTTCAATACAG+TGG + chr4_1:45839823-45839842 None:intergenic 30.0%
AGAACATTAAGGCTAAAAAG+TGG + chr4_1:45839865-45839884 None:intergenic 30.0%
ATATTAAACAGACCAAAACG+CGG + chr4_1:45839890-45839909 None:intergenic 30.0%
TTTAAGAGATGCTCATTTCT+AGG - chr4_1:45840013-45840032 Msa0550420:intron 30.0%
TTAAGAGATGCTCATTTCTA+GGG - chr4_1:45840014-45840033 Msa0550420:intron 30.0%
!! TTTCTAGGGCAATATGTATT+TGG - chr4_1:45840028-45840047 Msa0550420:intron 30.0%
! ATGTTTTCACAAAGTAACTG+AGG + chr4_1:45840075-45840094 None:intergenic 30.0%
GAGAAGATGAAATGTTAACT+AGG - chr4_1:45840182-45840201 Msa0550420:intron 30.0%
! TCTGGACTTGTTTATAAGTT+TGG - chr4_1:45840480-45840499 Msa0550420:intron 30.0%
GTTTGGCCTAATGTATTATT+TGG - chr4_1:45840497-45840516 Msa0550420:intron 30.0%
TTAAAATCAGTATCACACCT+AGG + chr4_1:45840841-45840860 None:intergenic 30.0%
!!! GTGTGATACTGATTTTAAGT+TGG - chr4_1:45840843-45840862 Msa0550420:intron 30.0%
! CATCTATCTATGATCGTTTT+TGG - chr4_1:45840983-45841002 Msa0550420:CDS 30.0%
!!! TATGATCGTTTTTGGAATTG+TGG - chr4_1:45840991-45841010 Msa0550420:CDS 30.0%
!!! ATGATCGTTTTTGGAATTGT+GGG - chr4_1:45840992-45841011 Msa0550420:CDS 30.0%
!!! ATGGTTGCACTTTTAGTTTT+TGG - chr4_1:45841092-45841111 Msa0550420:intron 30.0%
GAAGAAGACTGAAATTTGAG+AGG - chr4_1:45837628-45837647 Msa0550420:three_prime_UTR 35.0%
! CCTGTATTTTGCTAACCTTT+TGG + chr4_1:45837841-45837860 None:intergenic 35.0%
CCAAAAGGTTAGCAAAATAC+AGG - chr4_1:45837838-45837857 Msa0550420:three_prime_UTR 35.0%
AAAAAATGCGCCAACTTACA+TGG - chr4_1:45837899-45837918 Msa0550420:three_prime_UTR 35.0%
TGAAAAAGGTCCATGTAAGT+TGG + chr4_1:45837912-45837931 None:intergenic 35.0%
GTGTGACAAAGAACTTCTAT+TGG - chr4_1:45838002-45838021 Msa0550420:CDS 35.0%
!! AGCTTCTTTCATTTTCAAGC+AGG + chr4_1:45838029-45838048 None:intergenic 35.0%
ACTTATAACTAGAAAACCGG+CGG + chr4_1:45838122-45838141 None:intergenic 35.0%
GAAACAAAAAAATACCCCAG+CGG + chr4_1:45838161-45838180 None:intergenic 35.0%
CATTGTTTCCTTGAAACACA+TGG + chr4_1:45838183-45838202 None:intergenic 35.0%
CATGTGTTTCAAGGAAACAA+TGG - chr4_1:45838181-45838200 Msa0550420:intron 35.0%
!!! TTTGGACCGAGTTTTGATTT+GGG + chr4_1:45838277-45838296 None:intergenic 35.0%
GGCACATAAAATTCATTCAC+AGG + chr4_1:45838680-45838699 None:intergenic 35.0%
CAAAAGATTAAGCGCATGTT+TGG + chr4_1:45839116-45839135 None:intergenic 35.0%
! TTTTGGCAAAATGATTGCTC+TGG - chr4_1:45839212-45839231 Msa0550420:intron 35.0%
! GGCTTTTTGAACAATCTCTT+TGG - chr4_1:45839233-45839252 Msa0550420:intron 35.0%
AAAGGAGAAAGTGAACAACA+TGG - chr4_1:45839395-45839414 Msa0550420:CDS 35.0%
TTCAGTTAAGAGCTTGTCAT+AGG + chr4_1:45839439-45839458 None:intergenic 35.0%
! TAATGCCTTTTCTTATGGTG+TGG - chr4_1:45839523-45839542 Msa0550420:intron 35.0%
GTATGCGTGCATTATTATGT+TGG - chr4_1:45839574-45839593 Msa0550420:intron 35.0%
AAAATGATGAGCATCAGGAT+AGG + chr4_1:45839675-45839694 None:intergenic 35.0%
CTTGAAAAATGATGAGCATC+AGG + chr4_1:45839680-45839699 None:intergenic 35.0%
!! TTTTTCAGTCCTAGTATTGG+TGG - chr4_1:45839730-45839749 Msa0550420:intron 35.0%
! CTCTTGTATTGTTGTTCAAG+TGG - chr4_1:45839776-45839795 Msa0550420:intron 35.0%
AAAACGCGGATAGAACATTA+AGG + chr4_1:45839876-45839895 None:intergenic 35.0%
! TTAATGTTCTATCCGCGTTT+TGG - chr4_1:45839875-45839894 Msa0550420:intron 35.0%
! GCCAGAATTTTTGTGCTAAA+TGG - chr4_1:45839927-45839946 Msa0550420:intron 35.0%
GCCATTTAGCACAAAAATTC+TGG + chr4_1:45839931-45839950 None:intergenic 35.0%
! ATTTTCGGAAACAGGGAAAA+TGG + chr4_1:45840247-45840266 None:intergenic 35.0%
! AACTGCAATTTTCGGAAACA+GGG + chr4_1:45840254-45840273 None:intergenic 35.0%
! TAACTGCAATTTTCGGAAAC+AGG + chr4_1:45840255-45840274 None:intergenic 35.0%
AAACATCACTGTAAATGCAG+AGG + chr4_1:45840395-45840414 None:intergenic 35.0%
!! TAGGTGTCATTCTAAGTTTC+TGG - chr4_1:45840462-45840481 Msa0550420:intron 35.0%
TTGTGGTCTATGCAGATTAA+TGG - chr4_1:45840714-45840733 Msa0550420:intron 35.0%
! AGATTAATGGATCACTTGCA+AGG - chr4_1:45840727-45840746 Msa0550420:intron 35.0%
! ATAAGAGACTTGATGGCTAA+AGG - chr4_1:45840756-45840775 Msa0550420:intron 35.0%
TGATGGCTAAAGGTTTAATC+AGG - chr4_1:45840766-45840785 Msa0550420:intron 35.0%
AATCCATACCAATTGGCAAT+TGG - chr4_1:45841063-45841082 Msa0550420:intron 35.0%
AAACCAATTGCCAATTGGTA+TGG + chr4_1:45841069-45841088 None:intergenic 35.0%
ATCAGAAACCAATTGCCAAT+TGG + chr4_1:45841074-45841093 None:intergenic 35.0%
! AATTGGCAATTGGTTTCTGA+TGG - chr4_1:45841073-45841092 Msa0550420:intron 35.0%
!!! TTTTGGTTCATGATTTGGTC+AGG - chr4_1:45841109-45841128 Msa0550420:intron 35.0%
ATAAACTCCTGTGTGAATTG+TGG - chr4_1:45841216-45841235 Msa0550420:intron 35.0%
! TTTTTGTTGACGGAAGCTGA+CGG - chr4_1:45837803-45837822 Msa0550420:three_prime_UTR 40.0%
TCAAACTGACACCTACACTT+CGG - chr4_1:45838227-45838246 Msa0550420:intron 40.0%
!!! GTTTGGACCGAGTTTTGATT+TGG + chr4_1:45838278-45838297 None:intergenic 40.0%
CTTCTTAAAGCTACGTCGTT+TGG + chr4_1:45838295-45838314 None:intergenic 40.0%
TTATCTTCCTTAATGCCGTG+TGG - chr4_1:45839171-45839190 Msa0550420:intron 40.0%
AACTTTACCACACGGCATTA+AGG + chr4_1:45839181-45839200 None:intergenic 40.0%
TGTTTAACAGAAGTGGAGCA+AGG - chr4_1:45839365-45839384 Msa0550420:CDS 40.0%
GTTTAACAGAAGTGGAGCAA+GGG - chr4_1:45839366-45839385 Msa0550420:CDS 40.0%
!! AACATGGTGCTGTTTGATCA+GGG - chr4_1:45839411-45839430 Msa0550420:CDS 40.0%
GGAGTGATGAGCTTGTATTT+GGG + chr4_1:45839465-45839484 None:intergenic 40.0%
AGGAGTGATGAGCTTGTATT+TGG + chr4_1:45839466-45839485 None:intergenic 40.0%
! GGCTCGTATTTTCTGATAAG+TGG - chr4_1:45839797-45839816 Msa0550420:intron 40.0%
CTAGGGCAATATGTATTTGG+AGG - chr4_1:45840031-45840050 Msa0550420:intron 40.0%
!! GGAGGTGTTTCTGAGAATAT+AGG - chr4_1:45840049-45840068 Msa0550420:intron 40.0%
! TTAATGGATCACTTGCAAGG+AGG - chr4_1:45840730-45840749 Msa0550420:intron 40.0%
! TAATGGATCACTTGCAAGGA+GGG - chr4_1:45840731-45840750 Msa0550420:intron 40.0%
GGCTAAAGGTTTAATCAGGT+TGG - chr4_1:45840770-45840789 Msa0550420:intron 40.0%
AAGTCAGCAGATTTACACCA+GGG - chr4_1:45840806-45840825 Msa0550420:intron 40.0%
!! AAGTTGGAGAGTATCAGTAG+AGG - chr4_1:45840859-45840878 Msa0550420:intron 40.0%
CGCAAGGTATGGCATAGATT+GGG - chr4_1:45837589-45837608 Msa0550420:three_prime_UTR 45.0%
GCAAGGTATGGCATAGATTG+GGG - chr4_1:45837590-45837609 Msa0550420:three_prime_UTR 45.0%
TAGATTGGGGAAAGTCTGAG+AGG - chr4_1:45837603-45837622 Msa0550420:three_prime_UTR 45.0%
CAACGGTAGTTAATTACCGC+CGG - chr4_1:45838103-45838122 Msa0550420:intron 45.0%
AGTGGTAGTTTACTACCGCT+GGG - chr4_1:45838143-45838162 Msa0550420:intron 45.0%
! CGTGAGGCATTTAACCTACT+GGG + chr4_1:45838253-45838272 None:intergenic 45.0%
CCAGTAGGTTAAATGCCTCA+CGG - chr4_1:45838251-45838270 Msa0550420:intron 45.0%
TCACGGCCCAAATCAAAACT+CGG - chr4_1:45838268-45838287 Msa0550420:intron 45.0%
AGGTATGAGAAGGTCTGAGA+CGG + chr4_1:45838389-45838408 None:intergenic 45.0%
TCTCAGACCTTCTCATACCT+CGG - chr4_1:45838389-45838408 Msa0550420:intron 45.0%
ATACCTCGGAGCAGAAATCA+TGG - chr4_1:45838403-45838422 Msa0550420:intron 45.0%
!!! TTTTTTGCAGGCACCCAAGA+AGG - chr4_1:45838497-45838516 Msa0550420:intron 45.0%
!! CAACATGGTGCTGTTTGATC+AGG - chr4_1:45839410-45839429 Msa0550420:CDS 45.0%
AAACGATCAGAGAGGACAGA+AGG + chr4_1:45839486-45839505 None:intergenic 45.0%
TGTCCTCTCTGATCGTTTGA+GGG - chr4_1:45839488-45839507 Msa0550420:intron 45.0%
TTACCCTCAAACGATCAGAG+AGG + chr4_1:45839494-45839513 None:intergenic 45.0%
CATCACTGTAAATGCAGAGG+TGG + chr4_1:45840392-45840411 None:intergenic 45.0%
TCTAGTGTGATGCACACCTT+TGG - chr4_1:45840588-45840607 Msa0550420:intron 45.0%
! GTGAAAGTGCAAGCAACCAA+AGG + chr4_1:45840607-45840626 None:intergenic 45.0%
GAGGGCTATAAGAGACTTGA+TGG - chr4_1:45840749-45840768 Msa0550420:intron 45.0%
CAAGTCAGCAGATTTACACC+AGG - chr4_1:45840805-45840824 Msa0550420:intron 45.0%
!! TGGTTCATGATTTGGTCAGG+TGG - chr4_1:45841112-45841131 Msa0550420:intron 45.0%
!! GGTTCATGATTTGGTCAGGT+GGG - chr4_1:45841113-45841132 Msa0550420:intron 45.0%
!!! AAACAATTAAATTTAATTTT+TGG - chr4_1:45838813-45838832 Msa0550420:intron 5.0%
!!! AAAGTTTTTTTTTTTTTTTT+TGG - chr4_1:45839195-45839214 Msa0550420:intron 5.0%
!!! GGTTTTGGTTCAAAGGCGCA+AGG - chr4_1:45837573-45837592 Msa0550420:three_prime_UTR 50.0%
! TGGTTCAAAGGCGCAAGGTA+TGG - chr4_1:45837578-45837597 Msa0550420:three_prime_UTR 50.0%
GCGCAAGGTATGGCATAGAT+TGG - chr4_1:45837588-45837607 Msa0550420:three_prime_UTR 50.0%
ACACGTGGCAGTTCATGAGT+TGG + chr4_1:45837762-45837781 None:intergenic 50.0%
AAAAACGCCGTGGTGGCTAA+CGG + chr4_1:45837788-45837807 None:intergenic 50.0%
! CACCACGGCGTTTTTGTTGA+CGG - chr4_1:45837793-45837812 Msa0550420:three_prime_UTR 50.0%
TTCCGTCAACAAAAACGCCG+TGG + chr4_1:45837798-45837817 None:intergenic 50.0%
! CGGCAAGGACCTTTTCCAAA+AGG - chr4_1:45837823-45837842 Msa0550420:three_prime_UTR 50.0%
CAGTGGTAGTTTACTACCGC+TGG - chr4_1:45838142-45838161 Msa0550420:intron 50.0%
GTGGTAGTTTACTACCGCTG+GGG - chr4_1:45838144-45838163 Msa0550420:intron 50.0%
! CCGTGAGGCATTTAACCTAC+TGG + chr4_1:45838254-45838273 None:intergenic 50.0%
!!! GAGTTTTGATTTGGGCCGTG+AGG + chr4_1:45838269-45838288 None:intergenic 50.0%
! TATGAGAAGGTCTGAGACGG+AGG + chr4_1:45838386-45838405 None:intergenic 50.0%
TTCTGCTCCGAGGTATGAGA+AGG + chr4_1:45838399-45838418 None:intergenic 50.0%
ACACCATGATTTCTGCTCCG+AGG + chr4_1:45838409-45838428 None:intergenic 50.0%
CGGTGGCAAACAGAAGAAGA+AGG - chr4_1:45838557-45838576 Msa0550420:intron 50.0%
GTGGAGCAAGGGAAAGCAAA+AGG - chr4_1:45839377-45839396 Msa0550420:CDS 50.0%
CTGTCCTCTCTGATCGTTTG+AGG - chr4_1:45839487-45839506 Msa0550420:intron 50.0%
CGTCAACAAAAACGCCGTGG+TGG + chr4_1:45837795-45837814 None:intergenic 55.0%
AACCTACTGGGCCGAAGTGT+AGG + chr4_1:45838241-45838260 None:intergenic 55.0%
!! GATTTGGCTGGCTTGGAGGA+AGG + chr4_1:45838534-45838553 None:intergenic 55.0%
TCCTCCAAGCCAGCCAAATC+TGG - chr4_1:45838534-45838553 Msa0550420:intron 55.0%
TCCAAGCCAGCCAAATCTGG+CGG - chr4_1:45838537-45838556 Msa0550420:intron 55.0%
CTGTTTGCCACCGCCAGATT+TGG + chr4_1:45838550-45838569 None:intergenic 55.0%
CACCAGGGCTACAAACACCT+AGG - chr4_1:45840821-45840840 Msa0550420:intron 55.0%
CACCTAGGTGTTTGTAGCCC+TGG + chr4_1:45840826-45840845 None:intergenic 55.0%
GTGTGCTCCGTTAGCCACCA+CGG - chr4_1:45837778-45837797 Msa0550420:three_prime_UTR 60.0%
GTTGACGGAAGCTGACGGCA+AGG - chr4_1:45837808-45837827 Msa0550420:three_prime_UTR 60.0%
CACCTACACTTCGGCCCAGT+AGG - chr4_1:45838236-45838255 Msa0550420:intron 60.0%
! GAGGCGCTAGCTGCTATCAG+AGG + chr4_1:45838367-45838386 None:intergenic 60.0%
GGAGGAGGAGCTGCCTTCTT+GGG + chr4_1:45838513-45838532 None:intergenic 60.0%
AGGAGGAGGAGCTGCCTTCT+TGG + chr4_1:45838514-45838533 None:intergenic 60.0%
!! TTGGCTGGCTTGGAGGAAGG+AGG + chr4_1:45838531-45838550 None:intergenic 60.0%
!! GCCAGATTTGGCTGGCTTGG+AGG + chr4_1:45838538-45838557 None:intergenic 60.0%
! ACCGCCAGATTTGGCTGGCT+TGG + chr4_1:45838541-45838560 None:intergenic 60.0%
AAGCCAGCCAAATCTGGCGG+TGG - chr4_1:45838540-45838559 Msa0550420:intron 60.0%
TTGCCACCGCCAGATTTGGC+TGG + chr4_1:45838546-45838565 None:intergenic 60.0%
GGTGGCTAACGGAGCACACG+TGG + chr4_1:45837777-45837796 None:intergenic 65.0%
!! GCTGGCTTGGAGGAAGGAGG+AGG + chr4_1:45838528-45838547 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 45837570 45841241 45837570 ID=Msa0550420;Name=Msa0550420
chr4_1 mRNA 45837570 45841241 45837570 ID=Msa0550420-mRNA-1;Parent=Msa0550420;Name=Msa0550420-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0.6|0.5|0.66|1|1|6|399|149
chr4_1 exon 45837570 45838082 45837570 ID=Msa0550420-mRNA-1:exon:12128;Parent=Msa0550420-mRNA-1
chr4_1 exon 45839302 45839436 45839302 ID=Msa0550420-mRNA-1:exon:12127;Parent=Msa0550420-mRNA-1
chr4_1 exon 45840233 45840304 45840233 ID=Msa0550420-mRNA-1:exon:12126;Parent=Msa0550420-mRNA-1
chr4_1 exon 45840387 45840437 45840387 ID=Msa0550420-mRNA-1:exon:12125;Parent=Msa0550420-mRNA-1
chr4_1 exon 45840967 45841019 45840967 ID=Msa0550420-mRNA-1:exon:12124;Parent=Msa0550420-mRNA-1
chr4_1 exon 45841217 45841241 45841217 ID=Msa0550420-mRNA-1:exon:12123;Parent=Msa0550420-mRNA-1
chr4_1 CDS 45841217 45841241 45841217 ID=Msa0550420-mRNA-1:cds;Parent=Msa0550420-mRNA-1
chr4_1 CDS 45840967 45841019 45840967 ID=Msa0550420-mRNA-1:cds;Parent=Msa0550420-mRNA-1
chr4_1 CDS 45840387 45840437 45840387 ID=Msa0550420-mRNA-1:cds;Parent=Msa0550420-mRNA-1
chr4_1 CDS 45840233 45840304 45840233 ID=Msa0550420-mRNA-1:cds;Parent=Msa0550420-mRNA-1
chr4_1 CDS 45839302 45839436 45839302 ID=Msa0550420-mRNA-1:cds;Parent=Msa0550420-mRNA-1
chr4_1 CDS 45837969 45838082 45837969 ID=Msa0550420-mRNA-1:cds;Parent=Msa0550420-mRNA-1
chr4_1 three_prime_UTR 45837570 45837968 45837570 ID=Msa0550420-mRNA-1:three_prime_utr;Parent=Msa0550420-mRNA-1
Gene Sequence

>Msa0550420

ATGGGTTTTGGTTCAAAGGCGCAAGCCACCACGGCGTTTTTGTTGACGGAAGCTGACGGCAAGGACCTTTTCCAAAAGCAGCTAGCGCCTCCGTCTCAGACCTTCTCATACCTCGGAGCAGAAATCATGGCACCCAAGAAGGCAGCTCCTCCTCCTTCCTCCAAGCCAGCCAAATCTGGCGGTGGCAAACAGAAGAAGAAGAAGTGGAGCAAGGGAAAGCAAAAGGAGAAAGTGAACAACATGGTGCTGTTTGATCAGGGTACCTATGACAAGCTCTTAACTGAAGCACCCAAATACAAGCTCATCACTCCTTCTGTCCTCTCTGATCGTTTGAGGATTAATGGATCACTTGCAAGGAGGGCTATAAGAGACTTGATGGCTAAAGGTTTAATCAGGTTGGTGTCTGCTCATTCAAGTCAGCAGATTTACACCAGGGCTACAAACACCTAG
Protein sequence

>Msa0550420

MGFGSKAQATTAFLLTEADGKDLFQKQLAPPSQTFSYLGAEIMAPKKAAPPPSSKPAKSGGGKQKKKKWSKGKQKEKVNNMVLFDQGTYDKLLTEAPKYKLITPSVLSDRLRINGSLARRAIRDLMAKGLIRLVSAHSSQQIYTRATNT*