AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0257450


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0257450 MtrunA17_Chr6g0453811 100.000 65 0 0 1 65 1 65 6.66e-42 130
Msa0257450 MtrunA17_Chr4g0041861 90.769 65 6 0 1 65 1 65 3.30e-37 118
Msa0257450 MtrunA17_Chr6g0453911 81.538 65 11 1 1 65 1 64 1.49e-31 103
Msa0257450 MtrunA17_Chr8g0381481 83.673 49 8 0 1 49 1 49 8.05e-25 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0257450 AT5G03850.1 90.769 65 5 1 1 65 1 64 1.33e-35 114
Msa0257450 AT3G10090.1 90.769 65 5 1 1 65 1 64 1.33e-35 114
Msa0257450 AT5G64140.1 89.231 65 6 1 1 65 1 64 1.14e-34 112

Find 36 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGAAGTTGGTTGCTAAAA+TGG 0.117951 2_2:+53336506 None:intergenic
ACCTGCTATTCAAGGAAATT+AGG 0.247464 2_2:+53335095 None:intergenic
TGATTGATATTTGATTAGTT+TGG 0.323942 2_2:-53335064 Msa0257450:three_prime_UTR
TTCTTATAACCTGCTATTCA+AGG 0.363273 2_2:+53335087 None:intergenic
ACCTAATTTCCTTGAATAGC+AGG 0.397759 2_2:-53335096 Msa0257450:three_prime_UTR
GCTGTTGTTGTGAAAGTTAT+GGG 0.420809 2_2:-53335548 Msa0257450:CDS
AGGATGTCTCCCTCCCTAAC+TGG 0.425936 2_2:+53335437 None:intergenic
TGCTGTTGTTGTGAAAGTTA+TGG 0.441002 2_2:-53335549 Msa0257450:CDS
TTATGGGTCGTACTGGATCC+AGG 0.451262 2_2:-53335532 Msa0257450:CDS
AACCTGAGTGACTTGTCCCC+TGG 0.461687 2_2:+53335514 None:intergenic
GAATGTGAAGGGACCAGTTA+GGG 0.464736 2_2:-53335450 Msa0257450:CDS
AGCTCTTAAAACTGTTGTGT+TGG 0.468388 2_2:-53335314 Msa0257450:three_prime_UTR
TGTGACTTAGATATAAAATC+TGG 0.471929 2_2:-53335186 Msa0257450:three_prime_UTR
GGAATGTGAAGGGACCAGTT+AGG 0.473428 2_2:-53335451 Msa0257450:CDS
TAAAATGGAAAGTGCAGAAT+CGG 0.485027 2_2:+53336521 None:intergenic
TCTCAGACTCGAGGAGGGTA+AGG 0.488938 2_2:+53335417 None:intergenic
AGGAAATTAGGTTTGAGACT+TGG 0.499121 2_2:+53335107 None:intergenic
TATATGTTGTTCAATATGTC+AGG 0.499645 2_2:-53335163 Msa0257450:three_prime_UTR
GAGAGAAACGAACTTGAAGT+TGG 0.505451 2_2:+53336494 None:intergenic
GTGAAAGTTATGGGTCGTAC+TGG 0.530087 2_2:-53335539 Msa0257450:CDS
GCGAAGAAGCTAGCTGCGTA+TGG 0.560787 2_2:+53336559 None:intergenic
TGTGAAGGGACCAGTTAGGG+AGG 0.582336 2_2:-53335447 Msa0257450:CDS
ATCAGAATCGTCACATCATG+AGG 0.584464 2_2:-53335472 Msa0257450:CDS
TTGCTTCTCTCTCAGACTCG+AGG 0.586554 2_2:+53335408 None:intergenic
AAACATCGAGACTCTTCTAT+CGG 0.589119 2_2:+53335378 None:intergenic
CTTCTCTCTCAGACTCGAGG+AGG 0.606831 2_2:+53335411 None:intergenic
TATGGGTCGTACTGGATCCA+GGG 0.613108 2_2:-53335531 Msa0257450:CDS
GGAAAGTGCAGAATCGGCGT+GGG 0.619085 2_2:+53336527 None:intergenic
TCACATCATGAGGAATGTGA+AGG 0.626309 2_2:-53335462 Msa0257450:CDS
TGGAAAGTGCAGAATCGGCG+TGG 0.632732 2_2:+53336526 None:intergenic
CACATCATGAGGAATGTGAA+GGG 0.632733 2_2:-53335461 Msa0257450:CDS
ATCCAGGGGACAAGTCACTC+AGG 0.638739 2_2:-53335516 Msa0257450:CDS
TTCTCTCTCAGACTCGAGGA+GGG 0.644799 2_2:+53335412 None:intergenic
GTGAAGGGACCAGTTAGGGA+GGG 0.647685 2_2:-53335446 Msa0257450:CDS
ATGCTGTCTTGTTTATACGA+TGG 0.664216 2_2:-53335135 Msa0257450:three_prime_UTR
ATGGGTCGTACTGGATCCAG+GGG 0.674814 2_2:-53335530 Msa0257450:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTGTTTTAATTAATGA+AGG - chr2_2:53336409-53336428 Msa0257450:intron 10.0%
!!! TATTTGTTTTAATTAATGAA+GGG - chr2_2:53336410-53336429 Msa0257450:intron 10.0%
!!! TTATTTGTTTTAATTAATGA+AGG - chr2_2:53336409-53336428 Msa0257450:intron 10.0%
!!! TATTTGTTTTAATTAATGAA+GGG - chr2_2:53336410-53336429 Msa0257450:intron 10.0%
!! TAGTTTAATAAAAATCAAAG+AGG + chr2_2:53335786-53335805 None:intergenic 15.0%
!! AAATTATGATTTAAATAGTC+TGG - chr2_2:53335895-53335914 Msa0257450:intron 15.0%
!! TAGTTTAATAAAAATCAAAG+AGG + chr2_2:53335786-53335805 None:intergenic 15.0%
!! AAATTATGATTTAAATAGTC+TGG - chr2_2:53335895-53335914 Msa0257450:intron 15.0%
!! GCTAAAAATATAGATCAATA+AGG + chr2_2:53335189-53335208 None:intergenic 20.0%
!!! GATTTTTTTTTGGTTGATTT+AGG - chr2_2:53335264-53335283 Msa0257450:three_prime_UTR 20.0%
!! AGTTAAAAAAGTCATTTCTA+AGG + chr2_2:53335587-53335606 None:intergenic 20.0%
!!! ATCAACTTCACTTTATTTTT+TGG + chr2_2:53335862-53335881 None:intergenic 20.0%
!!! TGATTGATATTTGATTAGTT+TGG - chr2_2:53336572-53336591 Msa0257450:five_prime_UTR 20.0%
!! GCTAAAAATATAGATCAATA+AGG + chr2_2:53335189-53335208 None:intergenic 20.0%
!!! GATTTTTTTTTGGTTGATTT+AGG - chr2_2:53335264-53335283 Msa0257450:three_prime_UTR 20.0%
!! AGTTAAAAAAGTCATTTCTA+AGG + chr2_2:53335587-53335606 None:intergenic 20.0%
!!! ATCAACTTCACTTTATTTTT+TGG + chr2_2:53335862-53335881 None:intergenic 20.0%
!!! TTTTTTTGGTTGATTTAGGT+TGG - chr2_2:53335268-53335287 Msa0257450:three_prime_UTR 25.0%
!! TTTAAAATGTGTGAAAGATG+AGG - chr2_2:53335438-53335457 Msa0257450:CDS 25.0%
!! TGATTGCTATTAATTGTGTT+TGG - chr2_2:53335618-53335637 Msa0257450:intron 25.0%
!!! TTGATTGCTTATTTTGTGTT+TGG - chr2_2:53335811-53335830 Msa0257450:intron 25.0%
!!! GATAAAAAAGTGTTTTTCAG+TGG - chr2_2:53335931-53335950 Msa0257450:intron 25.0%
! ACACATTCACAATTGTTAAT+AGG + chr2_2:53336034-53336053 None:intergenic 25.0%
! TGTGACTTAGATATAAAATC+TGG - chr2_2:53336450-53336469 Msa0257450:intron 25.0%
! TATATGTTGTTCAATATGTC+AGG - chr2_2:53336473-53336492 Msa0257450:intron 25.0%
!!! TTGATATTTGATTAGTTTGG+AGG - chr2_2:53336575-53336594 Msa0257450:five_prime_UTR 25.0%
!!! TTTTTTTGGTTGATTTAGGT+TGG - chr2_2:53335268-53335287 Msa0257450:three_prime_UTR 25.0%
!! TTTAAAATGTGTGAAAGATG+AGG - chr2_2:53335438-53335457 Msa0257450:CDS 25.0%
!! TGATTGCTATTAATTGTGTT+TGG - chr2_2:53335618-53335637 Msa0257450:intron 25.0%
!!! TTGATTGCTTATTTTGTGTT+TGG - chr2_2:53335811-53335830 Msa0257450:intron 25.0%
!!! GATAAAAAAGTGTTTTTCAG+TGG - chr2_2:53335931-53335950 Msa0257450:intron 25.0%
! ACACATTCACAATTGTTAAT+AGG + chr2_2:53336034-53336053 None:intergenic 25.0%
! TGTGACTTAGATATAAAATC+TGG - chr2_2:53336450-53336469 Msa0257450:intron 25.0%
! TATATGTTGTTCAATATGTC+AGG - chr2_2:53336473-53336492 Msa0257450:intron 25.0%
! TAAAATGGAAAGTGCAGAAT+CGG + chr2_2:53335118-53335137 None:intergenic 30.0%
TTCTTATAACCTGCTATTCA+AGG + chr2_2:53336552-53336571 None:intergenic 30.0%
! TAAAATGGAAAGTGCAGAAT+CGG + chr2_2:53335118-53335137 None:intergenic 30.0%
TTCTTATAACCTGCTATTCA+AGG + chr2_2:53336552-53336571 None:intergenic 30.0%
! CTTGAAGTTGGTTGCTAAAA+TGG + chr2_2:53335133-53335152 None:intergenic 35.0%
TCAACAAATTAAGCTGAAGC+TGG + chr2_2:53335523-53335542 None:intergenic 35.0%
CAATTGTTAATAGGAGAGCA+AGG + chr2_2:53336025-53336044 None:intergenic 35.0%
TGCTGTTGTTGTGAAAGTTA+TGG - chr2_2:53336087-53336106 Msa0257450:intron 35.0%
GCTGTTGTTGTGAAAGTTAT+GGG - chr2_2:53336088-53336107 Msa0257450:intron 35.0%
! TCAGGTTAGAGTGAAGTTTT+TGG - chr2_2:53336138-53336157 Msa0257450:intron 35.0%
AAACATCGAGACTCTTCTAT+CGG + chr2_2:53336261-53336280 None:intergenic 35.0%
AGCTCTTAAAACTGTTGTGT+TGG - chr2_2:53336322-53336341 Msa0257450:intron 35.0%
!! ATGCTGTCTTGTTTATACGA+TGG - chr2_2:53336501-53336520 Msa0257450:intron 35.0%
! AGGAAATTAGGTTTGAGACT+TGG + chr2_2:53336532-53336551 None:intergenic 35.0%
ACCTAATTTCCTTGAATAGC+AGG - chr2_2:53336540-53336559 Msa0257450:five_prime_UTR 35.0%
ACCTGCTATTCAAGGAAATT+AGG + chr2_2:53336544-53336563 None:intergenic 35.0%
! CTTGAAGTTGGTTGCTAAAA+TGG + chr2_2:53335133-53335152 None:intergenic 35.0%
TCAACAAATTAAGCTGAAGC+TGG + chr2_2:53335523-53335542 None:intergenic 35.0%
CAATTGTTAATAGGAGAGCA+AGG + chr2_2:53336025-53336044 None:intergenic 35.0%
TGCTGTTGTTGTGAAAGTTA+TGG - chr2_2:53336087-53336106 Msa0257450:intron 35.0%
GCTGTTGTTGTGAAAGTTAT+GGG - chr2_2:53336088-53336107 Msa0257450:intron 35.0%
! TCAGGTTAGAGTGAAGTTTT+TGG - chr2_2:53336138-53336157 Msa0257450:intron 35.0%
AAACATCGAGACTCTTCTAT+CGG + chr2_2:53336261-53336280 None:intergenic 35.0%
AGCTCTTAAAACTGTTGTGT+TGG - chr2_2:53336322-53336341 Msa0257450:intron 35.0%
!! ATGCTGTCTTGTTTATACGA+TGG - chr2_2:53336501-53336520 Msa0257450:intron 35.0%
! AGGAAATTAGGTTTGAGACT+TGG + chr2_2:53336532-53336551 None:intergenic 35.0%
ACCTAATTTCCTTGAATAGC+AGG - chr2_2:53336540-53336559 Msa0257450:five_prime_UTR 35.0%
ACCTGCTATTCAAGGAAATT+AGG + chr2_2:53336544-53336563 None:intergenic 35.0%
GAGAGAAACGAACTTGAAGT+TGG + chr2_2:53335145-53335164 None:intergenic 40.0%
ATCAGAATCGTCACATCATG+AGG - chr2_2:53336164-53336183 Msa0257450:intron 40.0%
TCACATCATGAGGAATGTGA+AGG - chr2_2:53336174-53336193 Msa0257450:intron 40.0%
CACATCATGAGGAATGTGAA+GGG - chr2_2:53336175-53336194 Msa0257450:intron 40.0%
GAGAGAAACGAACTTGAAGT+TGG + chr2_2:53335145-53335164 None:intergenic 40.0%
ATCAGAATCGTCACATCATG+AGG - chr2_2:53336164-53336183 Msa0257450:intron 40.0%
TCACATCATGAGGAATGTGA+AGG - chr2_2:53336174-53336193 Msa0257450:intron 40.0%
CACATCATGAGGAATGTGAA+GGG - chr2_2:53336175-53336194 Msa0257450:intron 40.0%
GTGAATGTGTGTGTTTGAGC+AGG - chr2_2:53336044-53336063 Msa0257450:intron 45.0%
ATGTGTGTGTTTGAGCAGGA+TGG - chr2_2:53336048-53336067 Msa0257450:intron 45.0%
GTGAAAGTTATGGGTCGTAC+TGG - chr2_2:53336097-53336116 Msa0257450:intron 45.0%
GAATGTGAAGGGACCAGTTA+GGG - chr2_2:53336186-53336205 Msa0257450:intron 45.0%
GTGAATGTGTGTGTTTGAGC+AGG - chr2_2:53336044-53336063 Msa0257450:intron 45.0%
ATGTGTGTGTTTGAGCAGGA+TGG - chr2_2:53336048-53336067 Msa0257450:intron 45.0%
GTGAAAGTTATGGGTCGTAC+TGG - chr2_2:53336097-53336116 Msa0257450:intron 45.0%
GAATGTGAAGGGACCAGTTA+GGG - chr2_2:53336186-53336205 Msa0257450:intron 45.0%
!! TCAAAATAAAAAAATTAATA+TGG + chr2_2:53335246-53335265 None:intergenic 5.0%
!!! TTTTTATTTTGATTTTTTTT+TGG - chr2_2:53335254-53335273 Msa0257450:three_prime_UTR 5.0%
!! TCAAAATAAAAAAATTAATA+TGG + chr2_2:53335246-53335265 None:intergenic 5.0%
!!! TTTTTATTTTGATTTTTTTT+TGG - chr2_2:53335254-53335273 Msa0257450:three_prime_UTR 5.0%
TTATGGGTCGTACTGGATCC+AGG - chr2_2:53336104-53336123 Msa0257450:intron 50.0%
TATGGGTCGTACTGGATCCA+GGG - chr2_2:53336105-53336124 Msa0257450:intron 50.0%
GGAATGTGAAGGGACCAGTT+AGG - chr2_2:53336185-53336204 Msa0257450:intron 50.0%
TTCTCTCTCAGACTCGAGGA+GGG + chr2_2:53336227-53336246 None:intergenic 50.0%
TTGCTTCTCTCTCAGACTCG+AGG + chr2_2:53336231-53336250 None:intergenic 50.0%
TTATGGGTCGTACTGGATCC+AGG - chr2_2:53336104-53336123 Msa0257450:intron 50.0%
TATGGGTCGTACTGGATCCA+GGG - chr2_2:53336105-53336124 Msa0257450:intron 50.0%
GGAATGTGAAGGGACCAGTT+AGG - chr2_2:53336185-53336204 Msa0257450:intron 50.0%
TTCTCTCTCAGACTCGAGGA+GGG + chr2_2:53336227-53336246 None:intergenic 50.0%
TTGCTTCTCTCTCAGACTCG+AGG + chr2_2:53336231-53336250 None:intergenic 50.0%
GCGAAGAAGCTAGCTGCGTA+TGG + chr2_2:53335080-53335099 None:intergenic 55.0%
! GGAAAGTGCAGAATCGGCGT+GGG + chr2_2:53335112-53335131 None:intergenic 55.0%
! TGGAAAGTGCAGAATCGGCG+TGG + chr2_2:53335113-53335132 None:intergenic 55.0%
ATGGGTCGTACTGGATCCAG+GGG - chr2_2:53336106-53336125 Msa0257450:intron 55.0%
ATCCAGGGGACAAGTCACTC+AGG - chr2_2:53336120-53336139 Msa0257450:intron 55.0%
! AACCTGAGTGACTTGTCCCC+TGG + chr2_2:53336125-53336144 None:intergenic 55.0%
TGTGAAGGGACCAGTTAGGG+AGG - chr2_2:53336189-53336208 Msa0257450:intron 55.0%
GTGAAGGGACCAGTTAGGGA+GGG - chr2_2:53336190-53336209 Msa0257450:intron 55.0%
AGGATGTCTCCCTCCCTAAC+TGG + chr2_2:53336202-53336221 None:intergenic 55.0%
TCTCAGACTCGAGGAGGGTA+AGG + chr2_2:53336222-53336241 None:intergenic 55.0%
CTTCTCTCTCAGACTCGAGG+AGG + chr2_2:53336228-53336247 None:intergenic 55.0%
GCGAAGAAGCTAGCTGCGTA+TGG + chr2_2:53335080-53335099 None:intergenic 55.0%
! GGAAAGTGCAGAATCGGCGT+GGG + chr2_2:53335112-53335131 None:intergenic 55.0%
! TGGAAAGTGCAGAATCGGCG+TGG + chr2_2:53335113-53335132 None:intergenic 55.0%
ATGGGTCGTACTGGATCCAG+GGG - chr2_2:53336106-53336125 Msa0257450:intron 55.0%
ATCCAGGGGACAAGTCACTC+AGG - chr2_2:53336120-53336139 Msa0257450:intron 55.0%
! AACCTGAGTGACTTGTCCCC+TGG + chr2_2:53336125-53336144 None:intergenic 55.0%
TGTGAAGGGACCAGTTAGGG+AGG - chr2_2:53336189-53336208 Msa0257450:intron 55.0%
GTGAAGGGACCAGTTAGGGA+GGG - chr2_2:53336190-53336209 Msa0257450:intron 55.0%
AGGATGTCTCCCTCCCTAAC+TGG + chr2_2:53336202-53336221 None:intergenic 55.0%
TCTCAGACTCGAGGAGGGTA+AGG + chr2_2:53336222-53336241 None:intergenic 55.0%
CTTCTCTCTCAGACTCGAGG+AGG + chr2_2:53336228-53336247 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 53335064 53336594 53335064 ID=Msa0257450;Name=Msa0257450
chr2_2 mRNA 53335064 53336594 53335064 ID=Msa0257450-mRNA-1;Parent=Msa0257450;Name=Msa0257450-mRNA-1;_AED=0.12;_eAED=0.12;_QI=88|0|0.5|1|0|0.5|2|330|65
chr2_2 exon 53335064 53335591 53335064 ID=Msa0257450-mRNA-1:exon:16029;Parent=Msa0257450-mRNA-1
chr2_2 exon 53336507 53336594 53336507 ID=Msa0257450-mRNA-1:exon:16028;Parent=Msa0257450-mRNA-1
chr2_2 five_prime_UTR 53336507 53336594 53336507 ID=Msa0257450-mRNA-1:five_prime_utr;Parent=Msa0257450-mRNA-1
chr2_2 CDS 53335394 53335591 53335394 ID=Msa0257450-mRNA-1:cds;Parent=Msa0257450-mRNA-1
chr2_2 three_prime_UTR 53335064 53335393 53335064 ID=Msa0257450-mRNA-1:three_prime_utr;Parent=Msa0257450-mRNA-1
Gene Sequence

>Msa0257450

ATGGAATCACAGATTAAGCATGCTGTTGTTGTGAAAGTTATGGGTCGTACTGGATCCAGGGGACAAGTCACTCAGGTTAGAGTGAAGTTTTTGGATGATCAGAATCGTCACATCATGAGGAATGTGAAGGGACCAGTTAGGGAGGGAGACATCCTTACCCTCCTCGAGTCTGAGAGAGAAGCAAGAAGACTCCGATAG

Protein sequence

>Msa0257450

MESQIKHAVVVKVMGRTGSRGQVTQVRVKFLDDQNRHIMRNVKGPVREGDILTLLESEREARRLR*