AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0830090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 44 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATGTTGTCCTAACAATTA+AGG 0.168807 5_4:-3166164 None:intergenic
TCAGTTTACCCTTAATTGTT+AGG 0.300983 5_4:+3166155 Msa0830090:three_prime_UTR
CATGATATGTTAGCCTGTTC+TGG 0.307236 5_4:+3165930 Msa0830090:three_prime_UTR
TAGATCTCTGTCTCATCTTT+CGG 0.343914 5_4:-3165856 None:intergenic
AAACTGAAAGCATAAATAAA+AGG 0.357989 5_4:-3166139 None:intergenic
TATGTTGTCCTAACAATTAA+GGG 0.364409 5_4:-3166163 None:intergenic
TATTGGTGCATGTACTGTTT+TGG 0.389852 5_4:+3166049 Msa0830090:three_prime_UTR
CTTTCCATCTTGCTTGATGC+CGG 0.419505 5_4:+3165904 Msa0830090:three_prime_UTR
ATCTTGAGGTTTGGTTGGGA+GGG 0.425741 5_4:+3165960 Msa0830090:three_prime_UTR
GATCTTGAGGTTTGGTTGGG+AGG 0.451658 5_4:+3165959 Msa0830090:three_prime_UTR
CAAGATCATTACACCAGAAC+AGG 0.453287 5_4:-3165943 None:intergenic
GCAAATTTCCGCGGCAGGGA+AGG 0.455538 5_4:-3164716 None:intergenic
TCTTGAGGTTTGGTTGGGAG+GGG 0.455633 5_4:+3165961 Msa0830090:three_prime_UTR
GGTGTAATGATCTTGAGGTT+TGG 0.457532 5_4:+3165951 Msa0830090:three_prime_UTR
TAATGATCTTGAGGTTTGGT+TGG 0.461100 5_4:+3165955 Msa0830090:three_prime_UTR
CGATGGCAAATTTCCGCGGC+AGG 0.470724 5_4:-3164721 None:intergenic
AATGATCTTGAGGTTTGGTT+GGG 0.481359 5_4:+3165956 Msa0830090:three_prime_UTR
ATGAAAATCATGTGGATGAG+GGG 0.489922 5_4:+3166275 Msa0830090:three_prime_UTR
ACATGAAAATCATGTGGATG+AGG 0.501805 5_4:+3166273 Msa0830090:three_prime_UTR
AGCGTATGAGGCGGTTGAAG+AGG 0.522163 5_4:+3165828 Msa0830090:CDS
GTTCTGGTGTAATGATCTTG+AGG 0.522478 5_4:+3165946 Msa0830090:three_prime_UTR
AATTACCTTGGCTCTCATGT+TGG 0.522645 5_4:-3164777 None:intergenic
GATGAGACAGAGATCTAAGT+AGG 0.529953 5_4:+3165862 Msa0830090:exon
ATCTAAGTAGGTGCATATGC+AGG 0.532333 5_4:+3165874 Msa0830090:exon
TTCAGTTCCCTTCCCTGCCG+CGG 0.533826 5_4:+3164708 Msa0830090:CDS
AGTGGAAGAAGAAGCGTATG+AGG 0.535090 5_4:+3165816 Msa0830090:intron
CACTGGGTTAGCTTAAGATG+TGG 0.544623 5_4:+3166212 Msa0830090:three_prime_UTR
CATGAAAATCATGTGGATGA+GGG 0.544921 5_4:+3166274 Msa0830090:three_prime_UTR
CAAATTTCCGCGGCAGGGAA+GGG 0.545646 5_4:-3164715 None:intergenic
GAAGCCGGCATCAAGCAAGA+TGG 0.582153 5_4:-3165908 None:intergenic
AGGCTAACATATCATGAAGC+CGG 0.585518 5_4:-3165923 None:intergenic
GTGCTGTGTTTCGTAAGAAG+CGG 0.597751 5_4:-3164650 None:intergenic
ATCTTAAGCTAACCCAGTGA+AGG 0.623767 5_4:-3166208 None:intergenic
TGAAAATCATGTGGATGAGG+GGG 0.624026 5_4:+3166276 Msa0830090:three_prime_UTR
ATCATCCAACATGAGAGCCA+AGG 0.635896 5_4:+3164772 Msa0830090:CDS
CTGAAAGCATAAATAAAAGG+AGG 0.640654 5_4:-3166136 None:intergenic
TTAAATACATGAAAATCATG+TGG 0.661283 5_4:+3166267 Msa0830090:three_prime_UTR
GATGGCAAATTTCCGCGGCA+GGG 0.670700 5_4:-3164720 None:intergenic
CACTATAAATACGAAAACGA+GGG 0.686259 5_4:+3164670 Msa0830090:CDS
GCACTATAAATACGAAAACG+AGG 0.689830 5_4:+3164669 Msa0830090:CDS
TCTCAAGTTTCTCTGTACGA+TGG 0.694510 5_4:-3164738 None:intergenic
TCAATTGTCTTAGAACAACA+AGG 0.700588 5_4:-3166009 None:intergenic
GGAAGAAGAAGCGTATGAGG+CGG 0.708289 5_4:+3165819 Msa0830090:CDS
TGTACGATGGCAAATTTCCG+CGG 0.709054 5_4:-3164725 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAAATGTTCAATATTTT+TGG + chr5_4:3164855-3164874 Msa0830090:intron 10.0%
!!! TTTAAATGTTCAATATTTTT+GGG + chr5_4:3164856-3164875 Msa0830090:intron 10.0%
!! AAAAAATATTATTCATTTCT+GGG - chr5_4:3165182-3165201 None:intergenic 10.0%
!!! TTTTAAATGTTCAATATTTT+TGG + chr5_4:3164855-3164874 Msa0830090:intron 10.0%
!!! TTTAAATGTTCAATATTTTT+GGG + chr5_4:3164856-3164875 Msa0830090:intron 10.0%
!! AAAAAATATTATTCATTTCT+GGG - chr5_4:3165182-3165201 None:intergenic 10.0%
!! ATTAGATACAATTATGATTT+AGG + chr5_4:3164879-3164898 Msa0830090:intron 15.0%
!! TTAGATACAATTATGATTTA+GGG + chr5_4:3164880-3164899 Msa0830090:intron 15.0%
!! GAAAAAATATTATTCATTTC+TGG - chr5_4:3165183-3165202 None:intergenic 15.0%
!! TAAATTAAATGTGATTGTAT+TGG + chr5_4:3165286-3165305 Msa0830090:intron 15.0%
!! ATTAGATACAATTATGATTT+AGG + chr5_4:3164879-3164898 Msa0830090:intron 15.0%
!! TTAGATACAATTATGATTTA+GGG + chr5_4:3164880-3164899 Msa0830090:intron 15.0%
!! GAAAAAATATTATTCATTTC+TGG - chr5_4:3165183-3165202 None:intergenic 15.0%
!! TAAATTAAATGTGATTGTAT+TGG + chr5_4:3165286-3165305 Msa0830090:intron 15.0%
!! TTTATATAATCAATCGAAGA+AGG - chr5_4:3164806-3164825 None:intergenic 20.0%
!! AAAACACATCAAACATTAAA+CGG - chr5_4:3164934-3164953 None:intergenic 20.0%
!!! AGTCAATTTGATGATTTTTT+AGG + chr5_4:3165060-3165079 Msa0830090:intron 20.0%
!! ATCACTGTTTAAAAATACTT+GGG - chr5_4:3165208-3165227 None:intergenic 20.0%
!! AATCACTGTTTAAAAATACT+TGG - chr5_4:3165209-3165228 None:intergenic 20.0%
!!! AAACTGAAAGCATAAATAAA+AGG - chr5_4:3166142-3166161 None:intergenic 20.0%
!! TTAAATACATGAAAATCATG+TGG + chr5_4:3166267-3166286 Msa0830090:three_prime_UTR 20.0%
!! TTTATATAATCAATCGAAGA+AGG - chr5_4:3164806-3164825 None:intergenic 20.0%
!! AAAACACATCAAACATTAAA+CGG - chr5_4:3164934-3164953 None:intergenic 20.0%
!!! AGTCAATTTGATGATTTTTT+AGG + chr5_4:3165060-3165079 Msa0830090:intron 20.0%
!! ATCACTGTTTAAAAATACTT+GGG - chr5_4:3165208-3165227 None:intergenic 20.0%
!! AATCACTGTTTAAAAATACT+TGG - chr5_4:3165209-3165228 None:intergenic 20.0%
!!! AAACTGAAAGCATAAATAAA+AGG - chr5_4:3166142-3166161 None:intergenic 20.0%
!! TTAAATACATGAAAATCATG+TGG + chr5_4:3166267-3166286 Msa0830090:three_prime_UTR 20.0%
!! TACAATTATGATTTAGGGTT+TGG + chr5_4:3164885-3164904 Msa0830090:intron 25.0%
!! ACAATTATGATTTAGGGTTT+GGG + chr5_4:3164886-3164905 Msa0830090:intron 25.0%
! TTCACATAATCATTTCTATG+AGG - chr5_4:3164977-3164996 None:intergenic 25.0%
! ATAATAGCGGTAATTATCTA+TGG - chr5_4:3165320-3165339 None:intergenic 25.0%
!!! TGAGTTTTAACTGATAATAG+CGG - chr5_4:3165333-3165352 None:intergenic 25.0%
! CTTAAGAAATTACACAATCT+CGG - chr5_4:3165418-3165437 None:intergenic 25.0%
! TATATCATAAGGTTACAACA+TGG - chr5_4:3165487-3165506 None:intergenic 25.0%
! CATACAGAGTATATATCATA+AGG - chr5_4:3165498-3165517 None:intergenic 25.0%
! TATGATATATACTCTGTATG+CGG + chr5_4:3165498-3165517 Msa0830090:intron 25.0%
!!! CATTTTGTGGTTTTTAAGTA+GGG + chr5_4:3165559-3165578 Msa0830090:intron 25.0%
!!! TATGTATTTTTTTCCCATTG+TGG + chr5_4:3165727-3165746 Msa0830090:intron 25.0%
!!! TTTTGAGTGTTTTGTAGTAT+TGG + chr5_4:3166032-3166051 Msa0830090:three_prime_UTR 25.0%
! TATGTTGTCCTAACAATTAA+GGG - chr5_4:3166166-3166185 None:intergenic 25.0%
! ATATGTTGTCCTAACAATTA+AGG - chr5_4:3166167-3166186 None:intergenic 25.0%
!! TACAATTATGATTTAGGGTT+TGG + chr5_4:3164885-3164904 Msa0830090:intron 25.0%
!! ACAATTATGATTTAGGGTTT+GGG + chr5_4:3164886-3164905 Msa0830090:intron 25.0%
! TTCACATAATCATTTCTATG+AGG - chr5_4:3164977-3164996 None:intergenic 25.0%
! ATAATAGCGGTAATTATCTA+TGG - chr5_4:3165320-3165339 None:intergenic 25.0%
!!! TGAGTTTTAACTGATAATAG+CGG - chr5_4:3165333-3165352 None:intergenic 25.0%
! CTTAAGAAATTACACAATCT+CGG - chr5_4:3165418-3165437 None:intergenic 25.0%
! TATATCATAAGGTTACAACA+TGG - chr5_4:3165487-3165506 None:intergenic 25.0%
! CATACAGAGTATATATCATA+AGG - chr5_4:3165498-3165517 None:intergenic 25.0%
! TATGATATATACTCTGTATG+CGG + chr5_4:3165498-3165517 Msa0830090:intron 25.0%
!!! CATTTTGTGGTTTTTAAGTA+GGG + chr5_4:3165559-3165578 Msa0830090:intron 25.0%
!!! TATGTATTTTTTTCCCATTG+TGG + chr5_4:3165727-3165746 Msa0830090:intron 25.0%
!!! TTTTGAGTGTTTTGTAGTAT+TGG + chr5_4:3166032-3166051 Msa0830090:three_prime_UTR 25.0%
! TATGTTGTCCTAACAATTAA+GGG - chr5_4:3166166-3166185 None:intergenic 25.0%
! ATATGTTGTCCTAACAATTA+AGG - chr5_4:3166167-3166186 None:intergenic 25.0%
CACTATAAATACGAAAACGA+GGG + chr5_4:3164670-3164689 Msa0830090:CDS 30.0%
! ACAACCATGTTTCTATCATT+TGG - chr5_4:3165017-3165036 None:intergenic 30.0%
ACTGTTTAAAAATACTTGGG+CGG - chr5_4:3165205-3165224 None:intergenic 30.0%
AATTTATGACCAATGAACCT+CGG - chr5_4:3165253-3165272 None:intergenic 30.0%
CAATTCAGTTAATAAGCCAA+AGG - chr5_4:3165382-3165401 None:intergenic 30.0%
CGAGATTGTGTAATTTCTTA+AGG + chr5_4:3165416-3165435 Msa0830090:intron 30.0%
ACTTAAAAACCACAAAATGC+TGG - chr5_4:3165558-3165577 None:intergenic 30.0%
!!! GCATTTTGTGGTTTTTAAGT+AGG + chr5_4:3165558-3165577 Msa0830090:intron 30.0%
! CTTCATAACAGTATTTTGCA+TGG + chr5_4:3165654-3165673 Msa0830090:intron 30.0%
TTCATAACAGTATCATGCTT+TGG + chr5_4:3165694-3165713 Msa0830090:intron 30.0%
TCAATGATGTTATGTTTCAG+TGG + chr5_4:3165798-3165817 Msa0830090:intron 30.0%
TCAATTGTCTTAGAACAACA+AGG - chr5_4:3166012-3166031 None:intergenic 30.0%
! CTGAAAGCATAAATAAAAGG+AGG - chr5_4:3166139-3166158 None:intergenic 30.0%
TCAGTTTACCCTTAATTGTT+AGG + chr5_4:3166155-3166174 Msa0830090:three_prime_UTR 30.0%
! ATTTTATGATATGCCTTCAC+TGG + chr5_4:3166195-3166214 Msa0830090:three_prime_UTR 30.0%
! TTTTATGATATGCCTTCACT+GGG + chr5_4:3166196-3166215 Msa0830090:three_prime_UTR 30.0%
CACTATAAATACGAAAACGA+GGG + chr5_4:3164670-3164689 Msa0830090:CDS 30.0%
! ACAACCATGTTTCTATCATT+TGG - chr5_4:3165017-3165036 None:intergenic 30.0%
ACTGTTTAAAAATACTTGGG+CGG - chr5_4:3165205-3165224 None:intergenic 30.0%
AATTTATGACCAATGAACCT+CGG - chr5_4:3165253-3165272 None:intergenic 30.0%
CAATTCAGTTAATAAGCCAA+AGG - chr5_4:3165382-3165401 None:intergenic 30.0%
CGAGATTGTGTAATTTCTTA+AGG + chr5_4:3165416-3165435 Msa0830090:intron 30.0%
ACTTAAAAACCACAAAATGC+TGG - chr5_4:3165558-3165577 None:intergenic 30.0%
!!! GCATTTTGTGGTTTTTAAGT+AGG + chr5_4:3165558-3165577 Msa0830090:intron 30.0%
! CTTCATAACAGTATTTTGCA+TGG + chr5_4:3165654-3165673 Msa0830090:intron 30.0%
TTCATAACAGTATCATGCTT+TGG + chr5_4:3165694-3165713 Msa0830090:intron 30.0%
TCAATGATGTTATGTTTCAG+TGG + chr5_4:3165798-3165817 Msa0830090:intron 30.0%
TCAATTGTCTTAGAACAACA+AGG - chr5_4:3166012-3166031 None:intergenic 30.0%
! CTGAAAGCATAAATAAAAGG+AGG - chr5_4:3166139-3166158 None:intergenic 30.0%
TCAGTTTACCCTTAATTGTT+AGG + chr5_4:3166155-3166174 Msa0830090:three_prime_UTR 30.0%
! ATTTTATGATATGCCTTCAC+TGG + chr5_4:3166195-3166214 Msa0830090:three_prime_UTR 30.0%
! TTTTATGATATGCCTTCACT+GGG + chr5_4:3166196-3166215 Msa0830090:three_prime_UTR 30.0%
GCACTATAAATACGAAAACG+AGG + chr5_4:3164669-3164688 Msa0830090:CDS 35.0%
! ATGATTTAGGGTTTGGGTTT+AGG + chr5_4:3164892-3164911 Msa0830090:intron 35.0%
CGTTCCAAATGATAGAAACA+TGG + chr5_4:3165010-3165029 Msa0830090:intron 35.0%
TACTATAATCCGAGGTTCAT+TGG + chr5_4:3165241-3165260 Msa0830090:intron 35.0%
!!! TTTTTTCCCATTGTGGTTGT+TGG + chr5_4:3165734-3165753 Msa0830090:intron 35.0%
TAGATCTCTGTCTCATCTTT+CGG - chr5_4:3165859-3165878 None:intergenic 35.0%
! TAATGATCTTGAGGTTTGGT+TGG + chr5_4:3165955-3165974 Msa0830090:three_prime_UTR 35.0%
! AATGATCTTGAGGTTTGGTT+GGG + chr5_4:3165956-3165975 Msa0830090:three_prime_UTR 35.0%
! TATTGGTGCATGTACTGTTT+TGG + chr5_4:3166049-3166068 Msa0830090:three_prime_UTR 35.0%
ACATGAAAATCATGTGGATG+AGG + chr5_4:3166273-3166292 Msa0830090:three_prime_UTR 35.0%
CATGAAAATCATGTGGATGA+GGG + chr5_4:3166274-3166293 Msa0830090:three_prime_UTR 35.0%
ATGAAAATCATGTGGATGAG+GGG + chr5_4:3166275-3166294 Msa0830090:three_prime_UTR 35.0%
GCACTATAAATACGAAAACG+AGG + chr5_4:3164669-3164688 Msa0830090:CDS 35.0%
! ATGATTTAGGGTTTGGGTTT+AGG + chr5_4:3164892-3164911 Msa0830090:intron 35.0%
CGTTCCAAATGATAGAAACA+TGG + chr5_4:3165010-3165029 Msa0830090:intron 35.0%
TACTATAATCCGAGGTTCAT+TGG + chr5_4:3165241-3165260 Msa0830090:intron 35.0%
!!! TTTTTTCCCATTGTGGTTGT+TGG + chr5_4:3165734-3165753 Msa0830090:intron 35.0%
TAGATCTCTGTCTCATCTTT+CGG - chr5_4:3165859-3165878 None:intergenic 35.0%
! TAATGATCTTGAGGTTTGGT+TGG + chr5_4:3165955-3165974 Msa0830090:three_prime_UTR 35.0%
! AATGATCTTGAGGTTTGGTT+GGG + chr5_4:3165956-3165975 Msa0830090:three_prime_UTR 35.0%
! TATTGGTGCATGTACTGTTT+TGG + chr5_4:3166049-3166068 Msa0830090:three_prime_UTR 35.0%
ACATGAAAATCATGTGGATG+AGG + chr5_4:3166273-3166292 Msa0830090:three_prime_UTR 35.0%
CATGAAAATCATGTGGATGA+GGG + chr5_4:3166274-3166293 Msa0830090:three_prime_UTR 35.0%
ATGAAAATCATGTGGATGAG+GGG + chr5_4:3166275-3166294 Msa0830090:three_prime_UTR 35.0%
TCTCAAGTTTCTCTGTACGA+TGG - chr5_4:3164741-3164760 None:intergenic 40.0%
! AATTACCTTGGCTCTCATGT+TGG - chr5_4:3164780-3164799 None:intergenic 40.0%
CGAAGAAGGATGAATTACCT+TGG - chr5_4:3164792-3164811 None:intergenic 40.0%
TGACAGTGTACTATAATCCG+AGG + chr5_4:3165233-3165252 Msa0830090:intron 40.0%
ACACAATCTCGGCATCTTAA+TGG - chr5_4:3165407-3165426 None:intergenic 40.0%
GATATATACTCTGTATGCGG+AGG + chr5_4:3165501-3165520 Msa0830090:intron 40.0%
!!! TGGTTTTTAAGTAGGGCTAG+TGG + chr5_4:3165566-3165585 Msa0830090:intron 40.0%
TGTCAATGACTACCGTAGAA+TGG - chr5_4:3165631-3165650 None:intergenic 40.0%
ATCAAGCCAACAACCACAAT+GGG - chr5_4:3165743-3165762 None:intergenic 40.0%
AATCAAGCCAACAACCACAA+TGG - chr5_4:3165744-3165763 None:intergenic 40.0%
GATGAGACAGAGATCTAAGT+AGG + chr5_4:3165862-3165881 Msa0830090:exon 40.0%
ATCTAAGTAGGTGCATATGC+AGG + chr5_4:3165874-3165893 Msa0830090:exon 40.0%
AGGCTAACATATCATGAAGC+CGG - chr5_4:3165926-3165945 None:intergenic 40.0%
! CATGATATGTTAGCCTGTTC+TGG + chr5_4:3165930-3165949 Msa0830090:three_prime_UTR 40.0%
CAAGATCATTACACCAGAAC+AGG - chr5_4:3165946-3165965 None:intergenic 40.0%
GTTCTGGTGTAATGATCTTG+AGG + chr5_4:3165946-3165965 Msa0830090:three_prime_UTR 40.0%
GGTGTAATGATCTTGAGGTT+TGG + chr5_4:3165951-3165970 Msa0830090:three_prime_UTR 40.0%
ATCTTAAGCTAACCCAGTGA+AGG - chr5_4:3166211-3166230 None:intergenic 40.0%
TGAAAATCATGTGGATGAGG+GGG + chr5_4:3166276-3166295 Msa0830090:three_prime_UTR 40.0%
TCTCAAGTTTCTCTGTACGA+TGG - chr5_4:3164741-3164760 None:intergenic 40.0%
! AATTACCTTGGCTCTCATGT+TGG - chr5_4:3164780-3164799 None:intergenic 40.0%
CGAAGAAGGATGAATTACCT+TGG - chr5_4:3164792-3164811 None:intergenic 40.0%
TGACAGTGTACTATAATCCG+AGG + chr5_4:3165233-3165252 Msa0830090:intron 40.0%
ACACAATCTCGGCATCTTAA+TGG - chr5_4:3165407-3165426 None:intergenic 40.0%
GATATATACTCTGTATGCGG+AGG + chr5_4:3165501-3165520 Msa0830090:intron 40.0%
!!! TGGTTTTTAAGTAGGGCTAG+TGG + chr5_4:3165566-3165585 Msa0830090:intron 40.0%
TGTCAATGACTACCGTAGAA+TGG - chr5_4:3165631-3165650 None:intergenic 40.0%
ATCAAGCCAACAACCACAAT+GGG - chr5_4:3165743-3165762 None:intergenic 40.0%
AATCAAGCCAACAACCACAA+TGG - chr5_4:3165744-3165763 None:intergenic 40.0%
GATGAGACAGAGATCTAAGT+AGG + chr5_4:3165862-3165881 Msa0830090:exon 40.0%
ATCTAAGTAGGTGCATATGC+AGG + chr5_4:3165874-3165893 Msa0830090:exon 40.0%
AGGCTAACATATCATGAAGC+CGG - chr5_4:3165926-3165945 None:intergenic 40.0%
! CATGATATGTTAGCCTGTTC+TGG + chr5_4:3165930-3165949 Msa0830090:three_prime_UTR 40.0%
CAAGATCATTACACCAGAAC+AGG - chr5_4:3165946-3165965 None:intergenic 40.0%
GTTCTGGTGTAATGATCTTG+AGG + chr5_4:3165946-3165965 Msa0830090:three_prime_UTR 40.0%
GGTGTAATGATCTTGAGGTT+TGG + chr5_4:3165951-3165970 Msa0830090:three_prime_UTR 40.0%
ATCTTAAGCTAACCCAGTGA+AGG - chr5_4:3166211-3166230 None:intergenic 40.0%
TGAAAATCATGTGGATGAGG+GGG + chr5_4:3166276-3166295 Msa0830090:three_prime_UTR 40.0%
GTGCTGTGTTTCGTAAGAAG+CGG - chr5_4:3164653-3164672 None:intergenic 45.0%
TGTACGATGGCAAATTTCCG+CGG - chr5_4:3164728-3164747 None:intergenic 45.0%
ATCATCCAACATGAGAGCCA+AGG + chr5_4:3164772-3164791 Msa0830090:CDS 45.0%
! GTCTTTGAGCCAGCATTTTG+TGG + chr5_4:3165546-3165565 Msa0830090:intron 45.0%
CCTTCCTGATAGCCATTCTA+CGG + chr5_4:3165616-3165635 Msa0830090:intron 45.0%
ACTACCGTAGAATGGCTATC+AGG - chr5_4:3165623-3165642 None:intergenic 45.0%
! AGTGGAAGAAGAAGCGTATG+AGG + chr5_4:3165816-3165835 Msa0830090:intron 45.0%
CTTTCCATCTTGCTTGATGC+CGG + chr5_4:3165904-3165923 Msa0830090:three_prime_UTR 45.0%
! ATCTTGAGGTTTGGTTGGGA+GGG + chr5_4:3165960-3165979 Msa0830090:three_prime_UTR 45.0%
!! TGGTGCATGTACTGTTTTGG+TGG + chr5_4:3166052-3166071 Msa0830090:three_prime_UTR 45.0%
!! CACTGGGTTAGCTTAAGATG+TGG + chr5_4:3166212-3166231 Msa0830090:three_prime_UTR 45.0%
GTGCTGTGTTTCGTAAGAAG+CGG - chr5_4:3164653-3164672 None:intergenic 45.0%
TGTACGATGGCAAATTTCCG+CGG - chr5_4:3164728-3164747 None:intergenic 45.0%
ATCATCCAACATGAGAGCCA+AGG + chr5_4:3164772-3164791 Msa0830090:CDS 45.0%
! GTCTTTGAGCCAGCATTTTG+TGG + chr5_4:3165546-3165565 Msa0830090:intron 45.0%
CCTTCCTGATAGCCATTCTA+CGG + chr5_4:3165616-3165635 Msa0830090:intron 45.0%
ACTACCGTAGAATGGCTATC+AGG - chr5_4:3165623-3165642 None:intergenic 45.0%
! AGTGGAAGAAGAAGCGTATG+AGG + chr5_4:3165816-3165835 Msa0830090:intron 45.0%
CTTTCCATCTTGCTTGATGC+CGG + chr5_4:3165904-3165923 Msa0830090:three_prime_UTR 45.0%
! ATCTTGAGGTTTGGTTGGGA+GGG + chr5_4:3165960-3165979 Msa0830090:three_prime_UTR 45.0%
!! TGGTGCATGTACTGTTTTGG+TGG + chr5_4:3166052-3166071 Msa0830090:three_prime_UTR 45.0%
!! CACTGGGTTAGCTTAAGATG+TGG + chr5_4:3166212-3166231 Msa0830090:three_prime_UTR 45.0%
! ATAAGCCAAAGGCGCAATGC+TGG - chr5_4:3165371-3165390 None:intergenic 50.0%
CCGTAGAATGGCTATCAGGA+AGG - chr5_4:3165619-3165638 None:intergenic 50.0%
! GGAAGAAGAAGCGTATGAGG+CGG + chr5_4:3165819-3165838 Msa0830090:CDS 50.0%
! GATCTTGAGGTTTGGTTGGG+AGG + chr5_4:3165959-3165978 Msa0830090:three_prime_UTR 50.0%
! TCTTGAGGTTTGGTTGGGAG+GGG + chr5_4:3165961-3165980 Msa0830090:three_prime_UTR 50.0%
GAGGGGGCAAAAATGCTTTC+AGG + chr5_4:3166292-3166311 Msa0830090:three_prime_UTR 50.0%
! ATAAGCCAAAGGCGCAATGC+TGG - chr5_4:3165371-3165390 None:intergenic 50.0%
CCGTAGAATGGCTATCAGGA+AGG - chr5_4:3165619-3165638 None:intergenic 50.0%
! GGAAGAAGAAGCGTATGAGG+CGG + chr5_4:3165819-3165838 Msa0830090:CDS 50.0%
! GATCTTGAGGTTTGGTTGGG+AGG + chr5_4:3165959-3165978 Msa0830090:three_prime_UTR 50.0%
! TCTTGAGGTTTGGTTGGGAG+GGG + chr5_4:3165961-3165980 Msa0830090:three_prime_UTR 50.0%
CAAATTTCCGCGGCAGGGAA+GGG - chr5_4:3164718-3164737 None:intergenic 55.0%
GATGGCAAATTTCCGCGGCA+GGG - chr5_4:3164723-3164742 None:intergenic 55.0%
TGCTACCAGCATTGCGCCTT+TGG + chr5_4:3165363-3165382 Msa0830090:intron 55.0%
AGCGTATGAGGCGGTTGAAG+AGG + chr5_4:3165828-3165847 Msa0830090:CDS 55.0%
GAAGCCGGCATCAAGCAAGA+TGG - chr5_4:3165911-3165930 None:intergenic 55.0%
CAAATTTCCGCGGCAGGGAA+GGG - chr5_4:3164718-3164737 None:intergenic 55.0%
GATGGCAAATTTCCGCGGCA+GGG - chr5_4:3164723-3164742 None:intergenic 55.0%
TGCTACCAGCATTGCGCCTT+TGG + chr5_4:3165363-3165382 Msa0830090:intron 55.0%
AGCGTATGAGGCGGTTGAAG+AGG + chr5_4:3165828-3165847 Msa0830090:CDS 55.0%
GAAGCCGGCATCAAGCAAGA+TGG - chr5_4:3165911-3165930 None:intergenic 55.0%
TTCAGTTCCCTTCCCTGCCG+CGG + chr5_4:3164708-3164727 Msa0830090:CDS 60.0%
GCAAATTTCCGCGGCAGGGA+AGG - chr5_4:3164719-3164738 None:intergenic 60.0%
CGATGGCAAATTTCCGCGGC+AGG - chr5_4:3164724-3164743 None:intergenic 60.0%
TTCAGTTCCCTTCCCTGCCG+CGG + chr5_4:3164708-3164727 Msa0830090:CDS 60.0%
GCAAATTTCCGCGGCAGGGA+AGG - chr5_4:3164719-3164738 None:intergenic 60.0%
CGATGGCAAATTTCCGCGGC+AGG - chr5_4:3164724-3164743 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 3164646 3166312 3164646 ID=Msa0830090;Name=Msa0830090
chr5_4 mRNA 3164646 3166312 3164646 ID=Msa0830090-mRNA-1;Parent=Msa0830090;Name=Msa0830090-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|1|0.5|1|1|1|2|433|69
chr5_4 exon 3164646 3164793 3164646 ID=Msa0830090-mRNA-1:exon:1129;Parent=Msa0830090-mRNA-1
chr5_4 exon 3165818 3166312 3165818 ID=Msa0830090-mRNA-1:exon:1130;Parent=Msa0830090-mRNA-1
chr5_4 CDS 3164646 3164793 3164646 ID=Msa0830090-mRNA-1:cds;Parent=Msa0830090-mRNA-1
chr5_4 CDS 3165818 3165879 3165818 ID=Msa0830090-mRNA-1:cds;Parent=Msa0830090-mRNA-1
chr5_4 three_prime_UTR 3165880 3166312 3165880 ID=Msa0830090-mRNA-1:three_prime_utr;Parent=Msa0830090-mRNA-1
Gene Sequence

>Msa0830090

GGCTCCGCTTCTTACGAAACACAGCACTATAAATACGAAAACGAGGGTTTTTCACTCTCAGATTCAGTTCCCTTCCCTGCCGCGGAAATTTGCCATCGTACAGAGAAACTTGAGAGCGTAGCGTTCATCATCCAACATGAGAGCCAAGTGGAAGAAGAAGCGTATGAGGCGGTTGAAGAGGAAGCGCCGAAAGATGAGACAGAGATCTAA
Protein sequence

>Msa0830090

GSASYETQHYKYENEGFSLSDSVPFPAAEICHRTEKLESVAFIIQHESQVEEEAYEAVEEEAPKDETEI*