AlfalfaGEDB | MS.gene035057

M. sativa cultivar XinJiangDaYe / MS.gene035057

KEGG: -
GO: -
PFAM: PF08387.13

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035057.t1	XP_013467007.1	55.9	315	104	7	1	282	74	386	9.70E-82	313.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035057.t1	Q8H1R7	28.6	259	150	8	37	282	183	419	6.3e-13	76.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035057.t1	A0A072VGV2	55.9	315	104	7	1	282	74	386	7.0e-82	313.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene035057	MS.gene78164	PPI
MS.gene055754	MS.gene035057	PPI
MS.gene035057	MS.gene09990	PPI
MS.gene32300	MS.gene035057	PPI
MS.gene035057	MS.gene68084	PPI
MS.gene035057	MS.gene60518	PPI
MS.gene035057	MS.gene024564	PPI
MS.gene035057	MS.gene050535	PPI
MS.gene035057	MS.gene36809	PPI
MS.gene035057	MS.gene01653	PPI
MS.gene035057	MS.gene050470	PPI
MS.gene09993	MS.gene035057	PPI
MS.gene035057	MS.gene36943	PPI
MS.gene035057	MS.gene032379	PPI
MS.gene054896	MS.gene035057	PPI
MS.gene68081	MS.gene035057	PPI
MS.gene23706	MS.gene035057	PPI
MS.gene035057	MS.gene36763	PPI
MS.gene035057	MS.gene024262	PPI
MS.gene035057	MS.gene67914	PPI
MS.gene035057	MS.gene60522	PPI
MS.gene035057	MS.gene80861	PPI
MS.gene035057	MS.gene026015	PPI
MS.gene035057	MS.gene028491	PPI
MS.gene072244	MS.gene035057	PPI
MS.gene035057	MS.gene51793	PPI
MS.gene035057	MS.gene033694	PPI
MS.gene035057	MS.gene045772	PPI
MS.gene035057	MS.gene007794	PPI
MS.gene035057	MS.gene54960	PPI
MS.gene035057	MS.gene90228	PPI
MS.gene035057	MS.gene30044	PPI
MS.gene32305	MS.gene035057	PPI
MS.gene035057	MS.gene072003	PPI
MS.gene67914	MS.gene035057	PPI
MS.gene053586	MS.gene035057	PPI
MS.gene42267	MS.gene035057	PPI
MS.gene035057	MS.gene002112	PPI
MS.gene97791	MS.gene035057	PPI
MS.gene035057	MS.gene047136	PPI
MS.gene035057	MS.gene36752	PPI
MS.gene035057	MS.gene065373	PPI
MS.gene035057	MS.gene033695	PPI
MS.gene035057	MS.gene60520	PPI
MS.gene035057	MS.gene068017	PPI
MS.gene035057	MS.gene30261	PPI
MS.gene040253	MS.gene035057	PPI
MS.gene035057	MS.gene54959	PPI
MS.gene035057	MS.gene51795	PPI
MS.gene035057	MS.gene30057	PPI
MS.gene035057	MS.gene071257	PPI
MS.gene035057	MS.gene072231	PPI
MS.gene67917	MS.gene035057	PPI
MS.gene36764	MS.gene035057	PPI
MS.gene36942	MS.gene035057	PPI
MS.gene047137	MS.gene035057	PPI
MS.gene035057	MS.gene36807	PPI
MS.gene035057	MS.gene36743	PPI
MS.gene035057	MS.gene072000	PPI
MS.gene035057	MS.gene36810	PPI
MS.gene36804	MS.gene035057	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035057.t1	MTR_1g041245	55.873	315	104	7	1	282	74	386	4.02e-106	313
MS.gene035057.t1	MTR_7g034115	55.446	303	105	3	1	274	74	375	6.49e-105	310
MS.gene035057.t1	MTR_7g032380	53.333	300	86	4	10	282	84	356	6.73e-98	291
MS.gene035057.t1	MTR_7g032370	49.837	307	116	7	4	282	56	352	4.43e-84	256
MS.gene035057.t1	MTR_7g033915	50.836	299	121	13	1	281	74	364	6.05e-83	254
MS.gene035057.t1	MTR_7g033990	45.455	308	128	10	1	274	74	375	1.91e-74	233
MS.gene035057.t1	MTR_7g034120	42.006	319	137	9	1	282	1	308	4.27e-68	214
MS.gene035057.t1	MTR_8g085430	45.878	279	94	8	3	270	84	316	5.99e-62	199
MS.gene035057.t1	MTR_7g056433	37.931	319	155	11	2	282	76	389	3.62e-53	177
MS.gene035057.t1	MTR_7g088260	37.224	317	156	12	1	282	154	462	9.43e-53	178
MS.gene035057.t1	MTR_7g115270	37.195	328	152	8	2	279	76	399	1.31e-52	176
MS.gene035057.t1	MTR_3g082890	37.910	335	148	10	1	282	157	484	9.91e-52	176
MS.gene035057.t1	MTR_7g098510	38.365	318	153	10	1	282	81	391	1.18e-51	174
MS.gene035057.t1	MTR_0543s0010	37.143	315	144	12	4	281	152	449	1.12e-50	172
MS.gene035057.t1	MTR_7g098570	38.535	314	148	10	1	277	83	388	5.25e-49	169
MS.gene035057.t1	MTR_3g082890	34.595	370	149	11	1	282	77	441	1.25e-47	164
MS.gene035057.t1	MTR_5g012840	38.328	287	152	7	1	282	52	318	2.42e-46	158
MS.gene035057.t1	MTR_3g108560	36.278	317	155	11	1	281	83	388	2.79e-46	160
MS.gene035057.t1	MTR_7g098480	36.760	321	144	10	4	281	84	388	1.50e-45	160
MS.gene035057.t1	MTR_7g056420	39.322	295	151	11	2	276	73	359	2.10e-45	164
MS.gene035057.t1	MTR_7g056430	39.322	295	151	11	2	276	73	359	2.73e-45	163
MS.gene035057.t1	MTR_3g108150	36.709	316	154	9	4	281	79	386	3.34e-45	157
MS.gene035057.t1	MTR_5g013500	36.218	312	163	13	2	282	59	365	6.64e-45	155
MS.gene035057.t1	MTR_0492s0020	35.974	303	140	12	4	269	152	437	5.11e-44	155
MS.gene035057.t1	MTR_3g036250	35.238	315	149	10	1	279	68	363	4.67e-41	145
MS.gene035057.t1	MTR_5g008920	34.700	317	161	10	2	282	75	381	1.82e-39	142
MS.gene035057.t1	MTR_5g008950	34.505	313	153	12	10	282	65	365	2.01e-39	141
MS.gene035057.t1	MTR_3g087260	37.102	283	134	11	36	281	181	456	3.49e-39	142
MS.gene035057.t1	MTR_7g081290	50.714	140	61	5	146	282	122	256	1.14e-34	126
MS.gene035057.t1	MTR_2g020180	48.201	139	57	4	146	282	90	215	2.12e-34	124
MS.gene035057.t1	MTR_3g107160	32.722	327	157	12	1	282	75	383	6.65e-34	127
MS.gene035057.t1	MTR_3g040700	35.357	280	128	10	1	244	106	368	2.38e-33	126
MS.gene035057.t1	MTR_7g034110	32.491	277	164	7	1	272	74	332	2.55e-33	125
MS.gene035057.t1	MTR_3g109982	40.183	219	116	9	67	275	256	469	2.80e-33	129
MS.gene035057.t1	MTR_3g107120	30.619	307	165	8	10	279	93	388	1.41e-31	121
MS.gene035057.t1	MTR_7g056020	37.089	213	123	6	70	275	159	367	3.48e-31	122
MS.gene035057.t1	MTR_3g107100	33.770	305	142	12	29	282	103	398	4.04e-31	120
MS.gene035057.t1	MTR_5g054920	38.500	200	99	6	78	264	3	191	3.68e-30	112
MS.gene035057.t1	MTR_7g026500	32.381	315	162	11	9	281	89	394	1.32e-29	115
MS.gene035057.t1	MTR_3g023630	29.545	308	179	8	4	282	75	373	9.48e-29	113
MS.gene035057.t1	MTR_6g029350	34.783	207	116	7	81	280	2	196	4.82e-28	108
MS.gene035057.t1	MTR_2g007450	30.530	321	143	12	28	282	115	421	5.31e-28	111
MS.gene035057.t1	MTR_4g080610	33.210	271	150	14	30	281	98	356	5.91e-28	110
MS.gene035057.t1	MTR_6g022520	30.982	326	166	12	1	281	1	312	8.74e-28	109
MS.gene035057.t1	MTR_6g022500	30.645	310	170	13	9	282	176	476	1.05e-27	111
MS.gene035057.t1	MTR_3g105060	30.868	311	165	12	4	282	81	373	1.26e-27	110
MS.gene035057.t1	MTR_3g115430	46.212	132	59	5	156	282	1	125	1.42e-27	103
MS.gene035057.t1	MTR_3g467400	30.952	336	144	12	1	282	66	367	2.77e-27	108
MS.gene035057.t1	MTR_1g085120	29.503	322	162	13	9	282	167	471	5.84e-27	109
MS.gene035057.t1	MTR_1g085120	29.503	322	162	13	9	282	242	546	8.41e-27	109
MS.gene035057.t1	MTR_5g058100	30.696	316	160	13	9	281	84	383	1.28e-26	107
MS.gene035057.t1	MTR_7g034090	32.095	296	127	13	1	281	75	311	2.70e-26	107
MS.gene035057.t1	MTR_7g007080	30.690	290	149	9	28	280	103	377	4.25e-26	106
MS.gene035057.t1	MTR_1089s0010	29.688	320	165	11	6	282	77	379	5.77e-26	105
MS.gene035057.t1	MTR_6g040270	33.040	227	128	10	67	280	164	379	1.17e-25	104
MS.gene035057.t1	MTR_7g075820	33.816	207	118	5	81	280	198	392	1.23e-25	105
MS.gene035057.t1	MTR_7g056277	32.090	268	161	12	23	281	372	627	1.75e-25	105
MS.gene035057.t1	MTR_5g058000	38.679	212	107	8	80	281	176	374	5.35e-25	102
MS.gene035057.t1	MTR_5g007410	32.743	226	130	6	70	279	186	405	5.99e-25	103
MS.gene035057.t1	MTR_1g107385	35.193	233	115	11	67	280	206	421	1.24e-24	102
MS.gene035057.t1	MTR_4g039440	29.179	329	175	10	1	282	95	412	1.42e-24	102
MS.gene035057.t1	MTR_7g114710	30.868	311	157	10	4	282	80	364	2.91e-24	100
MS.gene035057.t1	MTR_7g056287	29.032	310	166	12	9	281	79	371	5.91e-24	100
MS.gene035057.t1	MTR_1g066830	27.215	316	173	9	10	281	84	386	1.58e-23	99.0
MS.gene035057.t1	MTR_3g099830	33.486	218	111	8	81	280	171	372	3.69e-23	97.8
MS.gene035057.t1	MTR_7g007340	34.722	216	118	8	70	276	150	351	4.48e-23	97.4
MS.gene035057.t1	MTR_7g083740	27.586	290	158	10	28	281	93	366	4.72e-23	97.4
MS.gene035057.t1	MTR_6g045333	26.480	321	173	10	10	281	139	445	1.42e-22	97.1
MS.gene035057.t1	MTR_5g039450	29.373	303	164	11	4	279	76	355	1.49e-22	95.9
MS.gene035057.t1	MTR_5g008910	28.966	290	150	12	28	282	34	302	1.96e-22	94.7
MS.gene035057.t1	MTR_2g007550	35.220	159	77	4	131	279	190	332	2.84e-22	95.1
MS.gene035057.t1	MTR_5g045170	29.114	316	172	10	8	280	83	389	3.32e-22	95.5
MS.gene035057.t1	MTR_3g071850	27.070	314	174	11	10	278	102	405	4.80e-22	96.3
MS.gene035057.t1	MTR_7g055943	31.677	322	157	17	9	282	115	421	9.38e-22	94.4
MS.gene035057.t1	MTR_3g107940	40.476	126	65	3	150	274	89	205	1.74e-21	92.8
MS.gene035057.t1	MTR_6g011460	28.615	325	167	12	4	282	116	421	6.77e-21	92.0
MS.gene035057.t1	MTR_5g073370	32.099	243	140	9	50	280	149	378	1.22e-20	90.9
MS.gene035057.t1	MTR_2g007360	30.303	231	111	11	81	280	161	372	1.30e-20	90.9
MS.gene035057.t1	MTR_5g073420	26.198	313	181	12	4	281	82	379	1.52e-20	90.5
MS.gene035057.t1	MTR_1g043170	50.000	86	39	2	198	282	6	88	1.89e-20	84.0
MS.gene035057.t1	MTR_2g007090	34.579	214	102	9	78	281	108	293	2.30e-20	89.0
MS.gene035057.t1	MTR_2g007400	30.804	224	116	9	81	282	197	403	4.04e-20	89.7
MS.gene035057.t1	MTR_1g108800	35.349	215	112	8	80	280	139	340	5.48e-20	88.6
MS.gene035057.t1	MTR_2g007610	37.419	155	81	4	129	280	8	149	5.87e-20	85.1
MS.gene035057.t1	MTR_3g026920	30.132	302	142	13	28	279	120	402	1.12e-19	88.2
MS.gene035057.t1	MTR_5g039470	27.336	289	148	11	4	246	55	327	1.23e-19	87.4
MS.gene035057.t1	MTR_2g008310	31.163	215	121	8	81	282	171	371	1.25e-19	88.2
MS.gene035057.t1	MTR_1g053025	28.936	235	124	9	70	279	170	386	1.56e-19	87.8
MS.gene035057.t1	MTR_3g094980	47.619	84	41	1	170	250	34	117	2.08e-19	83.6
MS.gene035057.t1	MTR_5g083890	29.866	298	143	12	28	271	106	391	2.50e-19	87.8
MS.gene035057.t1	MTR_2g005990	29.236	301	152	12	29	280	105	393	5.17e-19	86.3
MS.gene035057.t1	MTR_2g036470	27.640	322	165	11	11	281	91	395	6.76e-19	85.9
MS.gene035057.t1	MTR_2g008320	30.093	216	124	6	81	282	225	427	8.67e-19	85.9
MS.gene035057.t1	MTR_7g056280	30.374	214	124	4	81	279	367	570	9.37e-19	86.3
MS.gene035057.t1	MTR_3g106960	35.000	200	108	7	87	274	1	190	9.64e-19	83.6
MS.gene035057.t1	MTR_3g108560	32.000	225	108	9	1	190	83	297	1.61e-18	84.0
MS.gene035057.t1	MTR_3g113785	42.000	100	44	2	150	243	4	95	7.41e-18	77.4
MS.gene035057.t1	MTR_1g108820	31.416	226	116	8	80	280	173	384	1.10e-17	82.4
MS.gene035057.t1	MTR_4g096730	48.148	81	38	2	203	282	12	89	3.21e-17	75.5
MS.gene035057.t1	MTR_6g022360	29.386	228	141	7	67	281	187	407	3.44e-17	81.3
MS.gene035057.t1	MTR_4g098590	31.111	270	129	10	28	250	95	354	3.94e-17	80.9
MS.gene035057.t1	MTR_1377s0010	37.903	124	68	4	129	250	11	127	1.08e-16	75.5
MS.gene035057.t1	MTR_0009s0080	27.465	284	153	11	10	250	94	367	1.70e-16	79.3
MS.gene035057.t1	MTR_5g031970	25.658	304	172	8	8	280	74	354	2.54e-16	78.2
MS.gene035057.t1	MTR_8g014210	40.909	110	56	2	176	282	128	231	8.19e-16	75.1
MS.gene035057.t1	MTR_5g024060	33.083	133	79	4	142	272	74	198	1.24e-15	75.1
MS.gene035057.t1	MTR_3g108140	42.045	88	50	1	195	281	60	147	4.05e-15	72.0
MS.gene035057.t1	MTR_8g077460	27.869	305	170	12	10	282	89	375	7.17e-15	74.3
MS.gene035057.t1	MTR_7g007370	31.963	219	118	7	70	278	136	333	1.15e-14	73.6
MS.gene035057.t1	MTR_3g071790	27.374	179	113	6	78	243	27	201	1.44e-14	71.2
MS.gene035057.t1	MTR_7g021950	32.317	164	91	5	131	282	274	429	1.52e-14	73.6
MS.gene035057.t1	MTR_5g063950	26.871	294	150	11	33	279	84	359	1.63e-14	73.2
MS.gene035057.t1	MTR_7g022105	33.803	142	82	4	131	264	275	412	1.63e-14	73.6
MS.gene035057.t1	MTR_7g021990	32.317	164	91	5	131	282	274	429	1.66e-14	73.6
MS.gene035057.t1	MTR_3g113770	50.000	58	29	0	186	243	280	337	3.25e-13	69.3
MS.gene035057.t1	MTR_6g044572	31.690	142	84	4	147	282	254	388	7.57e-13	68.6
MS.gene035057.t1	MTR_5g033200	31.977	172	99	7	108	272	12	172	2.17e-12	64.7
MS.gene035057.t1	MTR_6g044560	31.690	142	84	4	147	282	430	564	2.90e-12	67.0
MS.gene035057.t1	MTR_8g058970	35.088	114	70	2	172	282	365	477	1.91e-11	64.3
MS.gene035057.t1	MTR_8g062010	34.783	138	79	5	148	282	301	430	1.95e-11	64.3
MS.gene035057.t1	MTR_8g058965	34.783	138	79	4	148	282	327	456	2.31e-11	64.3
MS.gene035057.t1	MTR_1g059860	33.945	109	66	3	135	243	209	311	3.13e-11	63.2
MS.gene035057.t1	MTR_1g053050	41.250	80	43	1	200	279	37	112	3.23e-11	59.7
MS.gene035057.t1	MTR_5g045510	27.273	319	160	13	4	280	82	370	3.35e-11	63.5
MS.gene035057.t1	MTR_5g080710	29.870	231	133	11	60	274	229	446	3.36e-11	63.5
MS.gene035057.t1	MTR_8g062310	31.618	136	86	3	148	282	299	428	5.03e-11	63.2
MS.gene035057.t1	MTR_4g024710	27.650	217	129	8	86	282	139	347	9.74e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035057.t1	AT4G26340	28.302	265	143	9	37	282	183	419	1.42e-15	76.6
MS.gene035057.t1	AT4G10400	27.011	348	178	13	2	281	69	408	9.37e-15	74.3
MS.gene035057.t1	AT4G10400	27.011	348	178	13	2	281	69	408	9.37e-15	74.3
MS.gene035057.t1	AT4G10400	27.011	348	178	13	2	281	69	408	9.37e-15	74.3
MS.gene035057.t1	AT1G32375	26.429	280	149	9	54	282	149	422	4.41e-14	72.4
MS.gene035057.t1	AT1G32375	26.429	280	149	9	54	282	149	422	4.41e-14	72.4
MS.gene035057.t1	AT3G26920	25.000	236	165	4	58	281	65	300	1.56e-12	67.0
MS.gene035057.t1	AT3G26920	26.347	167	122	1	116	281	173	339	1.91e-12	67.0
MS.gene035057.t1	AT3G26920	26.347	167	122	1	116	281	173	339	1.91e-12	67.0

Find 65 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCAGAATGAAAAGTTTAA+TGG	0.193470	7.4:+67178394	MS.gene035057:CDS
TCTGAAGGTTAGGACAATTT+TGG	0.205184	7.4:-67178715	None:intergenic
GCTCATAGGTTTGTTGGTTA+TGG	0.237649	7.4:+67178298	MS.gene035057:intron
CTCATAGGTTTGTTGGTTAT+GGG	0.238104	7.4:+67178299	MS.gene035057:intron
AGCTGAGATGAAAGGCATTC+TGG	0.265816	7.4:-67178907	None:intergenic
GCATGCAAATCCTCTAAAAT+AGG	0.278080	7.4:-67178349	None:intergenic
AAGATAATTTCATTAGTATC+TGG	0.326783	7.4:-67179062	None:intergenic
TTGGTTATGGGATTCTTAAA+AGG	0.333429	7.4:+67178311	MS.gene035057:CDS
ATCTCCATGTGAGTCAAATT+AGG	0.339910	7.4:-67178635	None:intergenic
TGTTGACAGAAGTCTAGTTT+TGG	0.366138	7.4:+67178841	MS.gene035057:intron
TCTGGTGCTCATAGGTTTGT+TGG	0.375139	7.4:+67178292	MS.gene035057:intron
ATCATTCCGCCTCAAATGTC+TGG	0.378237	7.4:+67178022	MS.gene035057:CDS
AAAGTTTCTACACACAAAAC+TGG	0.410426	7.4:+67178489	MS.gene035057:CDS
TATTTGAATTGACAAATCAT+TGG	0.414434	7.4:+67178660	MS.gene035057:CDS
TATTACTAACCCAAGAAAAC+AGG	0.421521	7.4:-67178465	None:intergenic
GAAAGGCATTCTGGAACAAT+TGG	0.424326	7.4:-67178898	None:intergenic
TGAAATTATCTTTGTGTCCA+AGG	0.436414	7.4:+67179073	MS.gene035057:CDS
ATGGTAAGATTCTGAAGGTT+AGG	0.439216	7.4:-67178725	None:intergenic
CAAATCATTGGCCTGGGAGG+TGG	0.452691	7.4:+67178672	MS.gene035057:CDS
ATGAAATTGCCGATGGCGAT+TGG	0.459401	7.4:+67178866	MS.gene035057:CDS
CTTTCCTAATTTGACTCACA+TGG	0.460924	7.4:+67178631	MS.gene035057:CDS
GGATCGGCAATGTGGGGTCA+CGG	0.465199	7.4:-67177998	None:intergenic
GAATTGACAAATCATTGGCC+TGG	0.475903	7.4:+67178665	MS.gene035057:CDS
GATCGGCAATGTGGGGTCAC+GGG	0.479982	7.4:-67177997	None:intergenic
GAAATTATCTTTGTGTCCAA+GGG	0.482391	7.4:+67179074	MS.gene035057:CDS
ATAAATTTACTGACATCGTC+CGG	0.482466	7.4:-67178065	None:intergenic
AATTGACAAATCATTGGCCT+GGG	0.486901	7.4:+67178666	MS.gene035057:CDS
CATCTCTTCTGATTTCAATC+CGG	0.487016	7.4:+67178046	MS.gene035057:CDS
TTCTTAAAAGGCTTCTCACT+CGG	0.494674	7.4:+67178323	MS.gene035057:CDS
ATTGGCCTGGGAGGTGGAAG+TGG	0.499335	7.4:+67178678	MS.gene035057:CDS
GACATTTGAGGCGGAATGAT+CGG	0.499803	7.4:-67178019	None:intergenic
AACAAATAAGACCTCGTGAA+TGG	0.500338	7.4:-67178744	None:intergenic
GTCAACCACTTCCACCTCCC+AGG	0.505184	7.4:-67178683	None:intergenic
CCCTGACCTTTCTCAATCAA+AGG	0.512479	7.4:-67179118	None:intergenic
AATGATCGGATCGGCAATGT+GGG	0.518451	7.4:-67178005	None:intergenic
CATGAAAATTAGTGCCTCAC+CGG	0.526903	7.4:+67179020	MS.gene035057:CDS
AATTAGGAAAGGTGGGAACC+TGG	0.531927	7.4:-67178619	None:intergenic
GTGAGTCAAATTAGGAAAGG+TGG	0.536674	7.4:-67178627	None:intergenic
AGGCATTCTGGAACAATTGG+TGG	0.549253	7.4:-67178895	None:intergenic
CTTAAGAACAGTGAGGATAC+CGG	0.550352	7.4:-67178180	None:intergenic
AATTGCCGATGGCGATTGGA+TGG	0.558181	7.4:+67178870	MS.gene035057:CDS
TGAGTCAAATTAGGAAAGGT+GGG	0.558768	7.4:-67178626	None:intergenic
AAGAGATGTCCAGACATTTG+AGG	0.568341	7.4:-67178031	None:intergenic
TTACATGTAGCAGAGCCCCT+TGG	0.569225	7.4:-67179090	None:intergenic
GAATGATCGGATCGGCAATG+TGG	0.574566	7.4:-67178006	None:intergenic
AATGAACAAGGGACATGTCA+AGG	0.579425	7.4:-67178124	None:intergenic
GAAAATTAGTGCCTCACCGG+TGG	0.580886	7.4:+67179023	MS.gene035057:CDS
TTTGGAAATGAAATTGCCGA+TGG	0.595655	7.4:+67178859	MS.gene035057:CDS
GAGGCAGGCATATGAATACC+AGG	0.596128	7.4:+67178601	MS.gene035057:intron
AGCATTGTATTCAAATCCAC+CGG	0.603410	7.4:-67179039	None:intergenic
TGGATCCATCCAATCGCCAT+CGG	0.607827	7.4:-67178875	None:intergenic
CCGGAACTGAAAACACATGC+AGG	0.610036	7.4:-67178161	None:intergenic
CATGTGAGTCAAATTAGGAA+AGG	0.610877	7.4:-67178630	None:intergenic
CGTGAATGGTAAGATTCTGA+AGG	0.613895	7.4:-67178730	None:intergenic
ACTTAAAGCTAAAATGAACA+AGG	0.622487	7.4:-67178136	None:intergenic
TGACAAATCATTGGCCTGGG+AGG	0.629232	7.4:+67178669	MS.gene035057:CDS
TTGAGGCGGAATGATCGGAT+CGG	0.651331	7.4:-67178014	None:intergenic
AAATTATCTTTGTGTCCAAG+GGG	0.673883	7.4:+67179075	MS.gene035057:CDS
AGATGTCCAGACATTTGAGG+CGG	0.674602	7.4:-67178028	None:intergenic
CTTAAAGCTAAAATGAACAA+GGG	0.692653	7.4:-67178135	None:intergenic
ATGATCGGATCGGCAATGTG+GGG	0.699294	7.4:-67178004	None:intergenic
ATTGATGCTGTTGTGCAACG+TGG	0.706300	7.4:+67178086	MS.gene035057:CDS
TGTATTCAAATCCACCGGTG+AGG	0.710988	7.4:-67179034	None:intergenic
TCAGAATCTTACCATTCACG+AGG	0.726669	7.4:+67178733	MS.gene035057:CDS
ACTTCAACTTAAGAACAGTG+AGG	0.768046	7.4:-67178187	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTTTGTACAAAATTT+TGG	+	chr7.4:67178544-67178563	MS.gene035057:intron	10.0%
!!	ACAAAAAAAAAAAGTTACAA+GGG	-	chr7.4:67178535-67178554	None:intergenic	15.0%
!!	TACAAAAAAAAAAAGTTACA+AGG	-	chr7.4:67178536-67178555	None:intergenic	15.0%
!!!	TTTTTTTTTTATCAAAACGA+TGG	-	chr7.4:67178807-67178826	None:intergenic	15.0%
!!	AAGATAATTTCATTAGTATC+TGG	-	chr7.4:67179065-67179084	None:intergenic	20.0%
!!	TATTTGAATTGACAAATCAT+TGG	+	chr7.4:67178660-67178679	MS.gene035057:CDS	20.0%
!!	TTTCAGAATGAAAAGTTTAA+TGG	+	chr7.4:67178394-67178413	MS.gene035057:CDS	20.0%
!!!	TTAAACTTTTCATTCTGAAA+AGG	-	chr7.4:67178394-67178413	None:intergenic	20.0%
!	ACTTAAAGCTAAAATGAACA+AGG	-	chr7.4:67178139-67178158	None:intergenic	25.0%
!	CTTAAAGCTAAAATGAACAA+GGG	-	chr7.4:67178138-67178157	None:intergenic	25.0%
	AAAGTTTCTACACACAAAAC+TGG	+	chr7.4:67178489-67178508	MS.gene035057:CDS	30.0%
	AAATTATCTTTGTGTCCAAG+GGG	+	chr7.4:67179075-67179094	MS.gene035057:CDS	30.0%
	AGCAATGGAAAATTGACATT+GGG	-	chr7.4:67178233-67178252	None:intergenic	30.0%
	ATAAATTTACTGACATCGTC+CGG	-	chr7.4:67178068-67178087	None:intergenic	30.0%
	GAAATTATCTTTGTGTCCAA+GGG	+	chr7.4:67179074-67179093	MS.gene035057:CDS	30.0%
	TATTACTAACCCAAGAAAAC+AGG	-	chr7.4:67178468-67178487	None:intergenic	30.0%
	TGAAATTATCTTTGTGTCCA+AGG	+	chr7.4:67179073-67179092	MS.gene035057:CDS	30.0%
	TTGGTTATGGGATTCTTAAA+AGG	+	chr7.4:67178311-67178330	MS.gene035057:CDS	30.0%
!	TCAATCTATTTTATGCCATC+TGG	+	chr7.4:67178274-67178293	MS.gene035057:intron	30.0%
!	TTTGAACATCCTGTTTTCTT+GGG	+	chr7.4:67178456-67178475	MS.gene035057:CDS	30.0%
!	TTTTGAACATCCTGTTTTCT+TGG	+	chr7.4:67178455-67178474	MS.gene035057:CDS	30.0%
!!	ATGTTTTAAGCTGAGATGAA+AGG	-	chr7.4:67178918-67178937	None:intergenic	30.0%
!!!	ATGAAGAGTTTTTTGAGCAA+TGG	-	chr7.4:67178248-67178267	None:intergenic	30.0%
	AAAACTGGTATGAATCCCAT+TGG	+	chr7.4:67178504-67178523	MS.gene035057:intron	35.0%
	AACAAATAAGACCTCGTGAA+TGG	-	chr7.4:67178747-67178766	None:intergenic	35.0%
	ACTTCAACTTAAGAACAGTG+AGG	-	chr7.4:67178190-67178209	None:intergenic	35.0%
	AGCATTGTATTCAAATCCAC+CGG	-	chr7.4:67179042-67179061	None:intergenic	35.0%
	ATCTCCATGTGAGTCAAATT+AGG	-	chr7.4:67178638-67178657	None:intergenic	35.0%
	CATCTCTTCTGATTTCAATC+CGG	+	chr7.4:67178046-67178065	MS.gene035057:CDS	35.0%
	CATGTGAGTCAAATTAGGAA+AGG	-	chr7.4:67178633-67178652	None:intergenic	35.0%
	CTTTCCTAATTTGACTCACA+TGG	+	chr7.4:67178631-67178650	MS.gene035057:CDS	35.0%
	GAGCAATGGAAAATTGACAT+TGG	-	chr7.4:67178234-67178253	None:intergenic	35.0%
	TGAGTCAAATTAGGAAAGGT+GGG	-	chr7.4:67178629-67178648	None:intergenic	35.0%
	TTCTTAAAAGGCTTCTCACT+CGG	+	chr7.4:67178323-67178342	MS.gene035057:CDS	35.0%
	TTTGGAAATGAAATTGCCGA+TGG	+	chr7.4:67178859-67178878	MS.gene035057:CDS	35.0%
!	AATTGACAAATCATTGGCCT+GGG	+	chr7.4:67178666-67178685	MS.gene035057:CDS	35.0%
!	ATGGTAAGATTCTGAAGGTT+AGG	-	chr7.4:67178728-67178747	None:intergenic	35.0%
!	TCTGAAGGTTAGGACAATTT+TGG	-	chr7.4:67178718-67178737	None:intergenic	35.0%
!	TGCTCACTGATAGCTTTTAA+AGG	+	chr7.4:67178937-67178956	MS.gene035057:CDS	35.0%
!!	CTCATAGGTTTGTTGGTTAT+GGG	+	chr7.4:67178299-67178318	MS.gene035057:intron	35.0%
!!	GCATGCAAATCCTCTAAAAT+AGG	-	chr7.4:67178352-67178371	None:intergenic	35.0%
!!	TGTTGACAGAAGTCTAGTTT+TGG	+	chr7.4:67178841-67178860	MS.gene035057:intron	35.0%
	AAGAGATGTCCAGACATTTG+AGG	-	chr7.4:67178034-67178053	None:intergenic	40.0%
	AATGAACAAGGGACATGTCA+AGG	-	chr7.4:67178127-67178146	None:intergenic	40.0%
	CATGAAAATTAGTGCCTCAC+CGG	+	chr7.4:67179020-67179039	MS.gene035057:CDS	40.0%
	CGTGAATGGTAAGATTCTGA+AGG	-	chr7.4:67178733-67178752	None:intergenic	40.0%
	CTTAAGAACAGTGAGGATAC+CGG	-	chr7.4:67178183-67178202	None:intergenic	40.0%
	GAATTGACAAATCATTGGCC+TGG	+	chr7.4:67178665-67178684	MS.gene035057:CDS	40.0%
	GTGAGTCAAATTAGGAAAGG+TGG	-	chr7.4:67178630-67178649	None:intergenic	40.0%
	TCAGAATCTTACCATTCACG+AGG	+	chr7.4:67178733-67178752	MS.gene035057:CDS	40.0%
!	TGCTTTTGTAACACATGCAG+AGG	+	chr7.4:67178582-67178601	MS.gene035057:intron	40.0%
!!	GAAAGGCATTCTGGAACAAT+TGG	-	chr7.4:67178901-67178920	None:intergenic	40.0%
!!	GCTCATAGGTTTGTTGGTTA+TGG	+	chr7.4:67178298-67178317	MS.gene035057:intron	40.0%
!!!	AGGACAATTTTGGAGCACTT+TGG	-	chr7.4:67178708-67178727	None:intergenic	40.0%
	AATGATCGGATCGGCAATGT+GGG	-	chr7.4:67178008-67178027	None:intergenic	45.0%
	AATTAGGAAAGGTGGGAACC+TGG	-	chr7.4:67178622-67178641	None:intergenic	45.0%
	ACAAACCTATGAGCACCAGA+TGG	-	chr7.4:67178292-67178311	None:intergenic	45.0%
	AGATGTCCAGACATTTGAGG+CGG	-	chr7.4:67178031-67178050	None:intergenic	45.0%
	AGTTACAAGGGATGAGCCAA+TGG	-	chr7.4:67178523-67178542	None:intergenic	45.0%
	ATCATTCCGCCTCAAATGTC+TGG	+	chr7.4:67178022-67178041	MS.gene035057:CDS	45.0%
	ATGAAATTGCCGATGGCGAT+TGG	+	chr7.4:67178866-67178885	MS.gene035057:CDS	45.0%
	ATTGATGCTGTTGTGCAACG+TGG	+	chr7.4:67178086-67178105	MS.gene035057:CDS	45.0%
	GTTACAAGGGATGAGCCAAT+GGG	-	chr7.4:67178522-67178541	None:intergenic	45.0%
	TCTGGTGCTCATAGGTTTGT+TGG	+	chr7.4:67178292-67178311	MS.gene035057:intron	45.0%
	TGTATTCAAATCCACCGGTG+AGG	-	chr7.4:67179037-67179056	None:intergenic	45.0%
	TTATGCCATCTGGTGCTCAT+AGG	+	chr7.4:67178284-67178303	MS.gene035057:intron	45.0%
	TTTGTAACACATGCAGAGGC+AGG	+	chr7.4:67178586-67178605	MS.gene035057:intron	45.0%
!	CCTGCATGTGTTTTCAGTTC+CGG	+	chr7.4:67178161-67178180	MS.gene035057:CDS	45.0%
!	GACATTTGAGGCGGAATGAT+CGG	-	chr7.4:67178022-67178041	None:intergenic	45.0%
!!	AGCTGAGATGAAAGGCATTC+TGG	-	chr7.4:67178910-67178929	None:intergenic	45.0%
!!	AGGCATTCTGGAACAATTGG+TGG	-	chr7.4:67178898-67178917	None:intergenic	45.0%
!!	CGGTGAGGCACTAATTTTCA+TGG	-	chr7.4:67179022-67179041	None:intergenic	45.0%
!!!	CACTCGGTGTCCTATTTTAG+AGG	+	chr7.4:67178339-67178358	MS.gene035057:CDS	45.0%
	AATTGCCGATGGCGATTGGA+TGG	+	chr7.4:67178870-67178889	MS.gene035057:CDS	50.0%
	ATGATCGGATCGGCAATGTG+GGG	-	chr7.4:67178007-67178026	None:intergenic	50.0%
	CCGGAACTGAAAACACATGC+AGG	-	chr7.4:67178164-67178183	None:intergenic	50.0%
	GAAAATTAGTGCCTCACCGG+TGG	+	chr7.4:67179023-67179042	MS.gene035057:CDS	50.0%
	GAATGATCGGATCGGCAATG+TGG	-	chr7.4:67178009-67178028	None:intergenic	50.0%
	GAGGCAGGCATATGAATACC+AGG	+	chr7.4:67178601-67178620	MS.gene035057:intron	50.0%
	TGGATCCATCCAATCGCCAT+CGG	-	chr7.4:67178878-67178897	None:intergenic	50.0%
	TTACATGTAGCAGAGCCCCT+TGG	-	chr7.4:67179093-67179112	None:intergenic	50.0%
	TTGAGGCGGAATGATCGGAT+CGG	-	chr7.4:67178017-67178036	None:intergenic	50.0%
!	TGACAAATCATTGGCCTGGG+AGG	+	chr7.4:67178669-67178688	MS.gene035057:CDS	50.0%
!	CAAATCATTGGCCTGGGAGG+TGG	+	chr7.4:67178672-67178691	MS.gene035057:CDS	55.0%
	GATCGGCAATGTGGGGTCAC+GGG	-	chr7.4:67178000-67178019	None:intergenic	60.0%
	GGATCGGCAATGTGGGGTCA+CGG	-	chr7.4:67178001-67178020	None:intergenic	60.0%
	GTCAACCACTTCCACCTCCC+AGG	-	chr7.4:67178686-67178705	None:intergenic	60.0%
!	ATTGGCCTGGGAGGTGGAAG+TGG	+	chr7.4:67178678-67178697	MS.gene035057:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	67177990	67179128	67177990	ID=MS.gene035057
chr7.4	mRNA	67177990	67179128	67177990	ID=MS.gene035057.t1;Parent=MS.gene035057
chr7.4	exon	67177990	67178201	67177990	ID=MS.gene035057.t1.exon1;Parent=MS.gene035057.t1
chr7.4	CDS	67177990	67178201	67177990	ID=cds.MS.gene035057.t1;Parent=MS.gene035057.t1
chr7.4	exon	67178306	67178510	67178306	ID=MS.gene035057.t1.exon2;Parent=MS.gene035057.t1
chr7.4	CDS	67178306	67178510	67178306	ID=cds.MS.gene035057.t1;Parent=MS.gene035057.t1
chr7.4	exon	67178602	67178754	67178602	ID=MS.gene035057.t1.exon3;Parent=MS.gene035057.t1
chr7.4	CDS	67178602	67178754	67178602	ID=cds.MS.gene035057.t1;Parent=MS.gene035057.t1
chr7.4	exon	67178850	67179128	67178850	ID=MS.gene035057.t1.exon4;Parent=MS.gene035057.t1
chr7.4	CDS	67178850	67179128	67178850	ID=cds.MS.gene035057.t1;Parent=MS.gene035057.t1

Gene Sequence

>MS.gene035057

ATGCTCGCCCGTGACCCCACATTGCCGATCCGATCATTCCGCCTCAAATGTCTGGACATCTCTTCTGATTTCAATCCGGACGATGTCAGTAAATTTATTGATGCTGTTGTGCAACGTGGAATTCAGAATCTCAACCTTGACATGTCCCTTGTTCATTTTAGCTTTAAGTTACCTGCATGTGTTTTCAGTTCCGGTATCCTCACTGTTCTTAAGTTGAAGTCGCTAAATATATACGATCTTCCCAATGTCAATTTTCCATTGCTCAAAAAACTCTTCATTTGTGTTCAATCTATTTTATGCCATCTGGTGCTCATAGGTTTGTTGGTTATGGGATTCTTAAAAGGCTTCTCACTCGGTGTCCTATTTTAGAGGATTTGCATGCAACAAATTTATTGATCATTCCTTTTCAGAATGAAAAGTTTAATGGCTTACACAAATTAGTGAGAGCAAATGTTTATTTTAAGTTTTTGAACATCCTGTTTTCTTGGGTTAGTAATACAAAGTTTCTACACACAAAACTGGTATGAATCCCATTGGCTCATCCCTTGTAACTTTTTTTTTTTGTACAAAATTTTGGTTGATTTTTAATGTTTGCTTTTGTAACACATGCAGAGGCAGGCATATGAATACCAGGTTCCCACCTTTCCTAATTTGACTCACATGGAGATTGTATTTGAATTGACAAATCATTGGCCTGGGAGGTGGAAGTGGTTGACCAAAGTGCTCCAAAATTGTCCTAACCTTCAGAATCTTACCATTCACGAGGTCTTATTTGTTAATGTTAAATATTGATCATTTTTTTAAAATTTTACTTCCATCGTTTTGATAAAAAAAAAAAAAAAATCGTCTGTTGTTGACAGAAGTCTAGTTTTGGAAATGAAATTGCCGATGGCGATTGGATGGATCCACCAATTGTTCCAGAATGCCTTTCATCTCAGCTTAAAACATGCTCACTGATAGCTTTTAAAGGCATGAAGTGTGAGTTACAGTTTGCAGAGTACATCTTGAAGAGTGCAAAAACACTGCATACCATGAAAATTAGTGCCTCACCGGTGGATTTGAATACAATGCTCCAGATACTAATGAAATTATCTTTGTGTCCAAGGGGCTCTGCTACATGTAAACTATCCTTTGATTGA

Protein sequence

>MS.gene035057.t1

MLARDPTLPIRSFRLKCLDISSDFNPDDVSKFIDAVVQRGIQNLNLDMSLVHFSFKLPACVFSSGILTVLKFVGYGILKRLLTRCPILEDLHATNLLIIPFQNEKFNGLHKLVRANVYFKFLNILFSWVSNTKFLHTKLRQAYEYQVPTFPNLTHMEIVFELTNHWPGRWKWLTKVLQNCPNLQNLTIHEKSSFGNEIADGDWMDPPIVPECLSSQLKTCSLIAFKGMKCELQFAEYILKSAKTLHTMKISASPVDLNTMLQILMKLSLCPRGSATCKLSFD