AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene30261

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30261.t1 MTR_7g033990 78.924 223 40 6 1 219 74 293 3.44e-107 316
MS.gene30261.t1 MTR_7g033915 67.117 222 50 5 1 219 74 275 1.40e-96 287
MS.gene30261.t1 MTR_7g034120 58.667 225 80 8 1 217 1 220 4.85e-79 241
MS.gene30261.t1 MTR_1g041245 43.723 231 108 8 1 220 74 293 2.73e-46 159
MS.gene30261.t1 MTR_5g012840 41.704 223 81 6 1 219 52 229 1.30e-43 150
MS.gene30261.t1 MTR_7g034115 40.789 228 116 7 1 219 74 291 4.09e-43 150
MS.gene30261.t1 MTR_7g034110 43.925 214 73 5 10 217 80 252 5.80e-38 136
MS.gene30261.t1 MTR_7g056433 42.241 232 114 11 1 220 75 298 7.14e-37 134
MS.gene30261.t1 MTR_7g032380 37.555 229 100 8 10 231 84 276 3.08e-36 132
MS.gene30261.t1 MTR_7g032370 38.565 223 112 7 4 219 56 260 3.02e-35 129
MS.gene30261.t1 MTR_5g013500 35.600 250 115 10 1 229 58 282 6.16e-34 126
MS.gene30261.t1 MTR_7g098570 38.618 246 122 11 1 233 83 312 3.52e-33 125
MS.gene30261.t1 MTR_7g098510 38.983 236 112 10 1 220 81 300 1.39e-32 123
MS.gene30261.t1 MTR_0492s0020 39.111 225 114 8 4 221 152 360 8.31e-32 121
MS.gene30261.t1 MTR_0543s0010 39.111 225 114 8 4 221 152 360 1.07e-31 121
MS.gene30261.t1 MTR_7g115270 37.975 237 126 10 2 219 76 310 1.51e-31 120
MS.gene30261.t1 MTR_3g108560 37.229 231 121 8 1 221 83 299 3.73e-31 119
MS.gene30261.t1 MTR_3g108560 37.445 227 118 8 1 217 83 295 4.49e-31 117
MS.gene30261.t1 MTR_7g033945 59.406 101 36 3 1 99 74 171 2.87e-30 111
MS.gene30261.t1 MTR_7g098480 38.866 247 112 14 4 233 84 308 2.52e-29 115
MS.gene30261.t1 MTR_3g087260 39.796 196 95 9 37 222 184 366 1.19e-27 110
MS.gene30261.t1 MTR_7g088260 35.217 230 126 8 1 219 154 371 6.31e-27 108
MS.gene30261.t1 MTR_3g108150 36.170 235 112 10 4 219 79 294 7.27e-27 107
MS.gene30261.t1 MTR_7g056430 37.281 228 99 9 4 219 75 270 9.11e-26 106
MS.gene30261.t1 MTR_7g056420 37.281 228 99 9 4 219 75 270 9.65e-26 106
MS.gene30261.t1 MTR_3g082890 33.735 249 125 9 1 220 157 394 2.96e-25 103
MS.gene30261.t1 MTR_3g082890 33.871 248 124 9 1 219 77 313 4.37e-25 103
MS.gene30261.t1 MTR_3g040700 35.345 232 115 10 1 219 106 315 1.40e-24 101
MS.gene30261.t1 MTR_3g036250 34.043 235 120 10 1 222 68 280 1.19e-23 98.2
MS.gene30261.t1 MTR_7g056287 32.389 247 142 9 9 248 79 307 1.37e-23 98.2
MS.gene30261.t1 MTR_7g056277 35.071 211 123 7 9 216 335 534 7.43e-23 97.8
MS.gene30261.t1 MTR_3g109982 39.881 168 86 7 65 221 222 385 4.43e-22 95.5
MS.gene30261.t1 MTR_3g109982 35.417 144 78 3 1 138 76 210 3.61e-15 75.1
MS.gene30261.t1 MTR_3g110010 39.655 174 85 8 65 222 222 391 1.99e-21 93.6
MS.gene30261.t1 MTR_3g110010 35.417 144 78 3 1 138 76 210 4.17e-15 75.1
MS.gene30261.t1 MTR_8g085430 39.241 158 81 5 1 151 82 231 1.95e-20 89.0
MS.gene30261.t1 MTR_3g071850 32.609 230 120 9 10 219 102 316 2.24e-20 90.5
MS.gene30261.t1 MTR_7g060470 33.476 233 116 10 10 219 85 301 3.06e-20 89.4
MS.gene30261.t1 MTR_7g026500 33.624 229 130 9 9 223 89 309 3.87e-20 89.0
MS.gene30261.t1 MTR_5g007410 33.333 231 118 9 10 219 102 317 9.88e-19 85.5
MS.gene30261.t1 MTR_7g007080 35.122 205 99 10 26 217 103 286 1.12e-18 84.7
MS.gene30261.t1 MTR_6g022500 33.476 233 123 10 9 225 176 392 2.67e-18 84.3
MS.gene30261.t1 MTR_6g045333 34.361 227 120 10 10 219 139 353 3.28e-18 84.0
MS.gene30261.t1 MTR_5g058000 35.683 227 118 9 9 225 83 291 4.75e-18 82.8
MS.gene30261.t1 MTR_5g073420 32.751 229 129 9 4 224 82 293 1.38e-17 81.6
MS.gene30261.t1 MTR_5g008950 33.333 228 115 10 10 219 65 273 1.58e-17 81.3
MS.gene30261.t1 MTR_7g056020 31.873 251 134 10 1 233 72 303 3.11e-17 81.3
MS.gene30261.t1 MTR_0009s0080 28.727 275 154 9 10 257 94 353 1.88e-16 78.6
MS.gene30261.t1 MTR_1g066830 32.627 236 123 9 10 225 84 303 2.14e-16 78.2
MS.gene30261.t1 MTR_6g011460 32.751 229 125 9 4 219 116 328 3.10e-16 77.8
MS.gene30261.t1 MTR_5g045170 32.075 212 116 7 28 224 107 305 7.57e-16 76.6
MS.gene30261.t1 MTR_5g073370 37.952 166 85 7 65 219 130 288 8.15e-16 76.6
MS.gene30261.t1 MTR_3g023630 30.131 229 138 9 4 223 75 290 3.32e-15 74.7
MS.gene30261.t1 MTR_5g058100 37.500 176 88 8 66 225 131 300 8.72e-15 73.6
MS.gene30261.t1 MTR_1g108820 30.435 230 121 9 40 235 93 317 1.01e-14 73.6
MS.gene30261.t1 MTR_7g007340 29.630 216 124 8 19 219 60 262 2.23e-14 72.4
MS.gene30261.t1 MTR_3g105060 31.628 215 122 9 12 218 91 288 2.43e-14 72.4
MS.gene30261.t1 MTR_1g108820 31.004 229 113 10 40 237 93 307 4.17e-14 71.2
MS.gene30261.t1 MTR_7g056417 39.333 150 73 7 66 198 10 158 6.09e-14 68.2
MS.gene30261.t1 MTR_3g107100 31.343 201 114 9 29 217 105 293 6.61e-14 71.2
MS.gene30261.t1 MTR_5g073340 33.728 169 92 6 67 219 106 270 1.63e-13 69.3
MS.gene30261.t1 MTR_1g108800 34.591 159 85 5 66 210 93 246 1.74e-13 69.7
MS.gene30261.t1 MTR_5g061680 30.769 234 113 11 4 226 82 277 2.80e-13 68.9
MS.gene30261.t1 MTR_3g099830 29.412 204 118 8 32 219 82 275 3.29e-13 68.9
MS.gene30261.t1 MTR_5g039450 32.075 159 85 6 88 226 131 286 8.16e-13 67.8
MS.gene30261.t1 MTR_7g007370 32.934 167 93 6 65 221 96 253 1.96e-12 66.6
MS.gene30261.t1 MTR_7g055943 31.278 227 124 9 9 219 115 325 4.95e-12 65.5
MS.gene30261.t1 MTR_2g008310 27.119 236 125 12 32 231 72 296 1.91e-11 63.9
MS.gene30261.t1 MTR_5g045510 33.766 154 84 6 4 152 82 222 2.79e-11 63.2
MS.gene30261.t1 MTR_5g061410 30.542 203 119 6 27 219 77 267 3.04e-11 62.4
MS.gene30261.t1 MTR_3g467400 35.185 162 86 9 9 154 76 234 4.37e-11 62.8
MS.gene30261.t1 MTR_7g056447 39.683 126 57 7 1 119 72 185 4.67e-11 62.0
MS.gene30261.t1 MTR_7g114710 28.151 238 138 8 4 236 80 289 5.16e-11 62.4
MS.gene30261.t1 MTR_2g008320 28.856 201 112 9 44 223 151 341 6.65e-11 62.4
MS.gene30261.t1 MTR_5g076150 34.555 191 89 8 67 225 26 212 7.45e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 48 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCGCCTAAAATGTGCTTCT+AGG 0.249566 7.3:-64570371 MS.gene30261:CDS
TTTCCAACCGAAAACAAATT+AGG 0.266013 7.3:-64570249 MS.gene30261:CDS
AATGTTTGCTCTGACCAAAT+TGG 0.301668 7.3:+64569984 None:intergenic
AGCTTATATTCACATCAAAT+TGG 0.310979 7.3:-64569760 MS.gene30261:CDS
GTTTAAACACTTGCCCAATT+TGG 0.316334 7.3:-64569998 MS.gene30261:CDS
CCTACTCAAAACTCTCCATT+TGG 0.354917 7.3:-64570133 MS.gene30261:CDS
TTGAAAATTGTGGAAATTGT+AGG 0.366817 7.3:+64570225 None:intergenic
CACGAAATATTCAATTTGCT+TGG 0.381703 7.3:-64569948 MS.gene30261:CDS
ATGTTTGCTCTGACCAAATT+GGG 0.383897 7.3:+64569985 None:intergenic
GCTTTCGTTGATTTCTTATG+TGG 0.397870 7.3:-64567557 MS.gene30261:CDS
CGTGGGGTCACGGGCAATCA+TGG 0.426384 7.3:+64570410 None:intergenic
CCGCCTAAAATGTGCTTCTA+GGG 0.431683 7.3:-64570370 MS.gene30261:CDS
TATTTCCAACTCCTCTAGAA+TGG 0.432132 7.3:+64570062 None:intergenic
TATGCAGGTTGATGGTGAAT+TGG 0.442331 7.3:-64567518 MS.gene30261:CDS
AAGGGGCAACGTGGGGTCAC+GGG 0.446926 7.3:+64570401 None:intergenic
GAAGGGGCAACGTGGGGTCA+CGG 0.460177 7.3:+64570400 None:intergenic
CAAGCAAATTGAATATTTCG+TGG 0.460201 7.3:+64569949 None:intergenic
ACATCATTTATGCAGGTTGA+TGG 0.462109 7.3:-64567526 MS.gene30261:CDS
AGAATTTCATCCTGGATTTG+CGG 0.462664 7.3:-64570290 MS.gene30261:CDS
ATTTCCAACTCCTCTAGAAT+GGG 0.472558 7.3:+64570063 None:intergenic
GAATTGCAACGCTTCCCAAA+TGG 0.474421 7.3:+64570118 None:intergenic
CTTCTAGGGGATGCGACCCA+CGG 0.498878 7.3:-64570356 MS.gene30261:CDS
TTCTAGCAACCACTTCCATC+GGG 0.500898 7.3:+64569733 None:intergenic
TGCTTGCACATCATTTATGC+AGG 0.501753 7.3:-64567533 MS.gene30261:CDS
AGTAGGGGAAAATCGATTTG+AGG 0.510436 7.3:+64570150 None:intergenic
GTTTCTAGAATTGAAAATTG+TGG 0.512806 7.3:+64570215 None:intergenic
TTGTCCCATTCTAGAGGAGT+TGG 0.515673 7.3:-64570067 MS.gene30261:CDS
CTACTCAAAACTCTCCATTT+GGG 0.518709 7.3:-64570132 MS.gene30261:CDS
AGGAACTCAGAATTTCATCC+TGG 0.521403 7.3:-64570298 MS.gene30261:CDS
AAAGATCGAAGGGGCAACGT+GGG 0.532704 7.3:+64570393 None:intergenic
TTTCTAGCAACCACTTCCAT+CGG 0.537475 7.3:+64569732 None:intergenic
TTCTAGGGGATGCGACCCAC+GGG 0.546002 7.3:-64570355 MS.gene30261:CDS
TGAAATTCAAGCAGAAGCTA+AGG 0.550707 7.3:-64567485 MS.gene30261:CDS
CATAAATACAACCTTGTGAA+GGG 0.570727 7.3:+64569676 None:intergenic
ACATAAATACAACCTTGTGA+AGG 0.576752 7.3:+64569675 None:intergenic
TTTCAATTCTAGAAACCTTG+TGG 0.578779 7.3:-64570208 MS.gene30261:CDS
CATCAAATTGGCGTACCCGA+TGG 0.581156 7.3:-64569748 MS.gene30261:CDS
CGCCTAAAATGTGCTTCTAG+GGG 0.582406 7.3:-64570369 MS.gene30261:CDS
CGAGGGTTGTCCCATTCTAG+AGG 0.590341 7.3:-64570073 MS.gene30261:CDS
ATTGGCGTACCCGATGGAAG+TGG 0.591989 7.3:-64569742 MS.gene30261:CDS
GAAAGATCGAAGGGGCAACG+TGG 0.602298 7.3:+64570392 None:intergenic
TCAAAATCTTACCCTTCACA+AGG 0.613722 7.3:-64569687 MS.gene30261:intron
CTTTCAACTTAAGAACCACA+AGG 0.615571 7.3:+64570193 None:intergenic
ACAAATCTATCGATATCCCG+TGG 0.619805 7.3:+64570339 None:intergenic
AAGATCGAAGGGGCAACGTG+GGG 0.623695 7.3:+64570394 None:intergenic
CAAATCTATCGATATCCCGT+GGG 0.657369 7.3:+64570340 None:intergenic
GTTCGCGCTGCTGTGCAACG+AGG 0.699890 7.3:-64570318 MS.gene30261:CDS
TTTAGGCGGAAAGATCGAAG+GGG 0.744221 7.3:+64570384 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTTTTTCTTTTTATG+TGG - chr7.3:64568269-64568288 MS.gene30261:intron 10.0%
!!! TACAATTTATTTTTTTTACT+AGG - chr7.3:64569979-64569998 MS.gene30261:CDS 10.0%
!! AAAAGGAAAATTACAATATT+TGG - chr7.3:64570218-64570237 MS.gene30261:CDS 15.0%
!! ATTATGAATGAAATGTATTA+TGG - chr7.3:64569067-64569086 MS.gene30261:intron 15.0%
!! ATTGTGTGTTTATATATTTA+AGG - chr7.3:64570289-64570308 MS.gene30261:CDS 15.0%
!! CTAATTTAACATTAGAAAAT+AGG + chr7.3:64568610-64568629 None:intergenic 15.0%
!! GAAATTAAGTTATTCACAAA+AGG - chr7.3:64570201-64570220 MS.gene30261:CDS 20.0%
!!! TAGTATTTTGAATAAGTGTA+GGG - chr7.3:64568901-64568920 MS.gene30261:intron 20.0%
!!! TTAAATAGGTTTTATGATTC+TGG - chr7.3:64568669-64568688 MS.gene30261:intron 20.0%
!!! TTCATTCATTTTATTGAAGA+AGG - chr7.3:64568562-64568581 MS.gene30261:intron 20.0%
! AACAAAAACAATTATCAGCA+AGG + chr7.3:64568723-64568742 None:intergenic 25.0%
! AAGATAAATCCATTAGCATT+TGG + chr7.3:64568511-64568530 None:intergenic 25.0%
! AATAAAACTTACCTGAAAGT+CGG + chr7.3:64568945-64568964 None:intergenic 25.0%
! AATATTGTGCTATCGAATAT+TGG - chr7.3:64569770-64569789 MS.gene30261:CDS 25.0%
! AGCTTATATTCACATCAAAT+TGG - chr7.3:64568119-64568138 MS.gene30261:intron 25.0%
! ATAATCTGTTATAAGTAGGA+GGG + chr7.3:64569572-64569591 None:intergenic 25.0%
! GAATGTTTGACTATAGAAAA+AGG - chr7.3:64570138-64570157 MS.gene30261:CDS 25.0%
! GACAAGTAATAAAAAACACA+GGG + chr7.3:64569028-64569047 None:intergenic 25.0%
! TGACAAGTAATAAAAAACAC+AGG + chr7.3:64569029-64569048 None:intergenic 25.0%
! TGCAATTACTATGAACTTAT+TGG + chr7.3:64570054-64570073 None:intergenic 25.0%
! TTGAAAATTGTGGAAATTGT+AGG + chr7.3:64567657-64567676 None:intergenic 25.0%
!! AAAGAAAGTTAGTATTGAGT+CGG + chr7.3:64569526-64569545 None:intergenic 25.0%
!! TCATTTTATTGAAGAAGGTT+AGG - chr7.3:64568567-64568586 MS.gene30261:intron 25.0%
!! TTCTAGAAGCATATAAGAAA+GGG + chr7.3:64569636-64569655 None:intergenic 25.0%
!! TTTCTAGAAGCATATAAGAA+AGG + chr7.3:64569637-64569656 None:intergenic 25.0%
!! TTTTCATAACTTAACTCACA+TGG - chr7.3:64568096-64568115 MS.gene30261:intron 25.0%
!!! ACTTTCAGGTAAGTTTTATT+AGG - chr7.3:64568945-64568964 MS.gene30261:intron 25.0%
!!! GTAGTATTTTGAATAAGTGT+AGG - chr7.3:64568900-64568919 MS.gene30261:intron 25.0%
!!! GTTTCTAGAATTGAAAATTG+TGG + chr7.3:64567667-64567686 None:intergenic 25.0%
!!! TCTCGGTCATATTTTTAAAT+AGG - chr7.3:64568655-64568674 MS.gene30261:intron 25.0%
AAAACAATTATCAGCAAGGA+AGG + chr7.3:64568719-64568738 None:intergenic 30.0%
ACATAAATACAACCTTGTGA+AGG + chr7.3:64568207-64568226 None:intergenic 30.0%
ACATGTTTATCTTGGACTTA+TGG - chr7.3:64569247-64569266 MS.gene30261:intron 30.0%
AGCGATAATCTGTTATAAGT+AGG + chr7.3:64569576-64569595 None:intergenic 30.0%
AGTAACTTTCAGCAGAAATT+AGG - chr7.3:64570087-64570106 MS.gene30261:CDS 30.0%
ATACAGTACAACAATGCATT+AGG - chr7.3:64568878-64568897 MS.gene30261:intron 30.0%
ATGACTTGACATGTTTATCT+TGG - chr7.3:64569239-64569258 MS.gene30261:intron 30.0%
CAAGCAAATTGAATATTTCG+TGG + chr7.3:64567933-64567952 None:intergenic 30.0%
CATAAATACAACCTTGTGAA+GGG + chr7.3:64568206-64568225 None:intergenic 30.0%
GATAATCTGTTATAAGTAGG+AGG + chr7.3:64569573-64569592 None:intergenic 30.0%
TAAAGTTAAATCCACTGAAG+AGG + chr7.3:64568483-64568502 None:intergenic 30.0%
TGTCAAGTCATTAGTGTATA+AGG + chr7.3:64569230-64569249 None:intergenic 30.0%
TTTCCAACCGAAAACAAATT+AGG - chr7.3:64567630-64567649 MS.gene30261:intron 30.0%
! AAAGTTTTTGCGTTTAGAAG+TGG - chr7.3:64567965-64567984 MS.gene30261:intron 30.0%
! ATAGATGCACTTTGTTTCTT+AGG - chr7.3:64568630-64568649 MS.gene30261:intron 30.0%
! ATTCAAGTTCTGCATCATAT+CGG + chr7.3:64568794-64568813 None:intergenic 30.0%
! CACGAAATATTCAATTTGCT+TGG - chr7.3:64567931-64567950 MS.gene30261:intron 30.0%
! GTCAAACATTCATTTTGCTA+CGG + chr7.3:64570129-64570148 None:intergenic 30.0%
! TAAATATTGGCTGATGTTGT+AGG - chr7.3:64569836-64569855 MS.gene30261:intron 30.0%
! TCTAGAAGCATATAAGAAAG+GGG + chr7.3:64569635-64569654 None:intergenic 30.0%
! TTTAAAGCACCAAATGCTAA+TGG - chr7.3:64568499-64568518 MS.gene30261:intron 30.0%
! TTTCAATTCTAGAAACCTTG+TGG - chr7.3:64567671-64567690 MS.gene30261:intron 30.0%
!! AGATTTTGATAAGCTTATCG+AGG - chr7.3:64567788-64567807 MS.gene30261:intron 30.0%
!! ATGCTTTTGAAAGATGGTAT+TGG - chr7.3:64567855-64567874 MS.gene30261:intron 30.0%
!! CTATGTATGCTTTTGAAAGA+TGG - chr7.3:64567849-64567868 MS.gene30261:intron 30.0%
!! GAAAATTGATAACGTGGTAA+TGG - chr7.3:64569340-64569359 MS.gene30261:intron 30.0%
!! GATTTTGATAAGCTTATCGA+GGG - chr7.3:64567789-64567808 MS.gene30261:intron 30.0%
!! TCAATGGAAAATTGATAACG+TGG - chr7.3:64569334-64569353 MS.gene30261:intron 30.0%
!!! AGGGTAAGATTTTGAAGTTT+AGG + chr7.3:64568187-64568206 None:intergenic 30.0%
!!! GGGTAAGATTTTGAAGTTTA+GGG + chr7.3:64568186-64568205 None:intergenic 30.0%
!!! TGTAGGACCTAATTTGTTTT+CGG + chr7.3:64567640-64567659 None:intergenic 30.0%
AAGGAAGTTTCACAAAGGTT+TGG + chr7.3:64568700-64568719 None:intergenic 35.0%
AATGTTTGCTCTGACCAAAT+TGG + chr7.3:64567898-64567917 None:intergenic 35.0%
ACAGATTATCGCTAGAAATC+AGG - chr7.3:64569583-64569602 MS.gene30261:intron 35.0%
ACATCAGCCAATATTTACCA+TGG + chr7.3:64569833-64569852 None:intergenic 35.0%
ACTATGAACTTATTGGAACC+TGG + chr7.3:64570047-64570066 None:intergenic 35.0%
AGAATTTCATCCTGGATTTG+CGG - chr7.3:64567589-64567608 MS.gene30261:intron 35.0%
AGTTCTATGACTCTATGACA+TGG - chr7.3:64569173-64569192 MS.gene30261:intron 35.0%
ATAGAACTAGCTGTTCATGT+AGG + chr7.3:64569161-64569180 None:intergenic 35.0%
ATCAGCCAATATTTACCATG+GGG + chr7.3:64569831-64569850 None:intergenic 35.0%
ATGAAAAAGTGATGAGCTCA+AGG - chr7.3:64569401-64569420 MS.gene30261:intron 35.0%
ATGACTCTATGACATGGTTA+AGG - chr7.3:64569179-64569198 MS.gene30261:intron 35.0%
ATGGCATTTATGAGGACTAT+TGG - chr7.3:64568312-64568331 MS.gene30261:intron 35.0%
ATGTTCTTAGTTGAGAGGAA+AGG + chr7.3:64568364-64568383 None:intergenic 35.0%
ATGTTTGCTCTGACCAAATT+GGG + chr7.3:64567897-64567916 None:intergenic 35.0%
CATCAGCCAATATTTACCAT+GGG + chr7.3:64569832-64569851 None:intergenic 35.0%
CATTTATGAGGACTATTGGA+AGG - chr7.3:64568316-64568335 MS.gene30261:intron 35.0%
CTACTCAAAACTCTCCATTT+GGG - chr7.3:64567747-64567766 MS.gene30261:intron 35.0%
CTCAACGAAATAGAGCTATA+GGG - chr7.3:64570169-64570188 MS.gene30261:CDS 35.0%
CTTTCAACTTAAGAACCACA+AGG + chr7.3:64567689-64567708 None:intergenic 35.0%
GGAGGGAATAAAATCAGTTA+GGG + chr7.3:64569614-64569633 None:intergenic 35.0%
GGTACAACAAGCATAAAACA+GGG + chr7.3:64569698-64569717 None:intergenic 35.0%
GTTTAAACACTTGCCCAATT+TGG - chr7.3:64567881-64567900 MS.gene30261:intron 35.0%
TATCCATCCTTATTACTGTC+TGG - chr7.3:64568751-64568770 MS.gene30261:intron 35.0%
TCAAAATCTTACCCTTCACA+AGG - chr7.3:64568192-64568211 MS.gene30261:intron 35.0%
TGAAATTCAAGCAGAAGCTA+AGG - chr7.3:64570394-64570413 MS.gene30261:CDS 35.0%
TGCTCGCTTATAGATTACAA+AGG - chr7.3:64568383-64568402 MS.gene30261:intron 35.0%
TGGATTTATCTTTGTGTCCA+CGG - chr7.3:64568519-64568538 MS.gene30261:intron 35.0%
TTATGGACATGGCATTTATG+AGG - chr7.3:64568304-64568323 MS.gene30261:intron 35.0%
! AAGTTAGTATTGAGTCGGTT+AGG + chr7.3:64569521-64569540 None:intergenic 35.0%
! ACATCATTTATGCAGGTTGA+TGG - chr7.3:64570353-64570372 MS.gene30261:CDS 35.0%
! ACTTTGTTTCTTAGGTCTCT+CGG - chr7.3:64568638-64568657 MS.gene30261:intron 35.0%
! AGGCAGTTTGGAATAATTTC+TGG + chr7.3:64568344-64568363 None:intergenic 35.0%
! TATTTCCAACTCCTCTAGAA+TGG + chr7.3:64567820-64567839 None:intergenic 35.0%
!! ATTTCCAACTCCTCTAGAAT+GGG + chr7.3:64567819-64567838 None:intergenic 35.0%
!! GCTTTCGTTGATTTCTTATG+TGG - chr7.3:64570322-64570341 MS.gene30261:CDS 35.0%
!! TGGAACCTGGTTATTTTCTA+CGG + chr7.3:64570034-64570053 None:intergenic 35.0%
!! TGTGGATTTTGACAGCTTTA+TGG - chr7.3:64568287-64568306 MS.gene30261:intron 35.0%
!! TTTTGACAGCTTTATGGACA+TGG - chr7.3:64568293-64568312 MS.gene30261:intron 35.0%
!!! AAATGGAGAGTTTTGAGTAG+GGG + chr7.3:64567747-64567766 None:intergenic 35.0%
!!! CAAATGGAGAGTTTTGAGTA+GGG + chr7.3:64567748-64567767 None:intergenic 35.0%
AAAACAGGGTCCAATAATGC+TGG + chr7.3:64569684-64569703 None:intergenic 40.0%
ACAAATCTATCGATATCCCG+TGG + chr7.3:64567543-64567562 None:intergenic 40.0%
ACAACTGCCAGACAGTAATA+AGG + chr7.3:64568761-64568780 None:intergenic 40.0%
AGAGAATCTCTCTATGGATC+AGG + chr7.3:64569498-64569517 None:intergenic 40.0%
AGGAACTCAGAATTTCATCC+TGG - chr7.3:64567581-64567600 MS.gene30261:intron 40.0%
ATATTGGCCATGGAGGATAT+CGG - chr7.3:64569786-64569805 MS.gene30261:CDS 40.0%
CAAATCTATCGATATCCCGT+GGG + chr7.3:64567542-64567561 None:intergenic 40.0%
CAAGGAAGGAAGTTTCACAA+AGG + chr7.3:64568705-64568724 None:intergenic 40.0%
CCTACTCAAAACTCTCCATT+TGG - chr7.3:64567746-64567765 MS.gene30261:intron 40.0%
CTGTTATAAGTAGGAGGGAA+AGG + chr7.3:64569567-64569586 None:intergenic 40.0%
CTTTGCCGTAGAAAATAACC+AGG - chr7.3:64570026-64570045 MS.gene30261:CDS 40.0%
GAAGCATATAAGAAAGGGGA+GGG + chr7.3:64569631-64569650 None:intergenic 40.0%
GAAGTTTCACAAAGGTTTGG+AGG + chr7.3:64568697-64568716 None:intergenic 40.0%
GAATGTGAGTGTGCTTTCAA+TGG - chr7.3:64569318-64569337 MS.gene30261:intron 40.0%
GATTTATCTTTGTGTCCACG+GGG - chr7.3:64568521-64568540 MS.gene30261:intron 40.0%
GCTCAACGAAATAGAGCTAT+AGG - chr7.3:64570168-64570187 MS.gene30261:CDS 40.0%
GGATTTATCTTTGTGTCCAC+GGG - chr7.3:64568520-64568539 MS.gene30261:intron 40.0%
GGCTCTACAATTGTGGAAAA+CGG - chr7.3:64569422-64569441 MS.gene30261:intron 40.0%
GGGAGGGAATAAAATCAGTT+AGG + chr7.3:64569615-64569634 None:intergenic 40.0%
GGGTACAACAAGCATAAAAC+AGG + chr7.3:64569699-64569718 None:intergenic 40.0%
GTAATGCTACTGCAATGAGA+CGG + chr7.3:64569962-64569981 None:intergenic 40.0%
GTCTTCTCTAATCGACACAA+AGG - chr7.3:64569122-64569141 MS.gene30261:intron 40.0%
GTTAGGAGAGAATCTCTCTA+TGG + chr7.3:64569504-64569523 None:intergenic 40.0%
TAGAGAACGAGCAACTCAAT+TGG + chr7.3:64568978-64568997 None:intergenic 40.0%
TATTGAACAAGTCTTGCCGA+TGG + chr7.3:64569938-64569957 None:intergenic 40.0%
TCAGCCAATATTTACCATGG+GGG + chr7.3:64569830-64569849 None:intergenic 40.0%
TGCTTGCACATCATTTATGC+AGG - chr7.3:64570346-64570365 MS.gene30261:CDS 40.0%
! AGAAGCATATAAGAAAGGGG+AGG + chr7.3:64569632-64569651 None:intergenic 40.0%
! ATTTTAGGCGGAAAGATCGA+AGG + chr7.3:64567500-64567519 None:intergenic 40.0%
! TTTCTAGCAACCACTTCCAT+CGG + chr7.3:64568150-64568169 None:intergenic 40.0%
! TTTTAGGCGGAAAGATCGAA+GGG + chr7.3:64567499-64567518 None:intergenic 40.0%
!! AGTAGGGGAAAATCGATTTG+AGG + chr7.3:64567732-64567751 None:intergenic 40.0%
!! ATCCCCTAGAAGCACATTTT+AGG + chr7.3:64567515-64567534 None:intergenic 40.0%
!! TATGCAGGTTGATGGTGAAT+TGG - chr7.3:64570361-64570380 MS.gene30261:CDS 40.0%
!!! CCAAATGGAGAGTTTTGAGT+AGG + chr7.3:64567749-64567768 None:intergenic 40.0%
!!! GGACCTAATTTGTTTTCGGT+TGG + chr7.3:64567636-64567655 None:intergenic 40.0%
AAGACCACGTGATAGAGCAT+GGG + chr7.3:64569720-64569739 None:intergenic 45.0%
AAGTAGGAGGGAAAGGTAGT+AGG + chr7.3:64569560-64569579 None:intergenic 45.0%
AGCTCAAGGCTCTACAATTG+TGG - chr7.3:64569415-64569434 MS.gene30261:intron 45.0%
AGTTGAGAGGAAAGGCAGTT+TGG + chr7.3:64568356-64568375 None:intergenic 45.0%
CCGCCTAAAATGTGCTTCTA+GGG - chr7.3:64567509-64567528 MS.gene30261:CDS 45.0%
CGAGCATGTTCTTAGTTGAG+AGG + chr7.3:64568369-64568388 None:intergenic 45.0%
CGCCTAAAATGTGCTTCTAG+GGG - chr7.3:64567510-64567529 MS.gene30261:CDS 45.0%
CTATCGAATATTGGCCATGG+AGG - chr7.3:64569779-64569798 MS.gene30261:CDS 45.0%
CTGCCAGACAGTAATAAGGA+TGG + chr7.3:64568757-64568776 None:intergenic 45.0%
CTTGCCCCCATGGTAAATAT+TGG - chr7.3:64569823-64569842 MS.gene30261:intron 45.0%
GAATTGCAACGCTTCCCAAA+TGG + chr7.3:64567764-64567783 None:intergenic 45.0%
GCACATGAGACCAGCATTAT+TGG - chr7.3:64569671-64569690 MS.gene30261:intron 45.0%
GGTCATTGTGCGCAATAAGT+TGG + chr7.3:64568453-64568472 None:intergenic 45.0%
GTGCTATCGAATATTGGCCA+TGG - chr7.3:64569776-64569795 MS.gene30261:CDS 45.0%
TAAGACCACGTGATAGAGCA+TGG + chr7.3:64569721-64569740 None:intergenic 45.0%
TCCGCCTAAAATGTGCTTCT+AGG - chr7.3:64567508-64567527 MS.gene30261:CDS 45.0%
TTCTAGCAACCACTTCCATC+GGG + chr7.3:64568149-64568168 None:intergenic 45.0%
TTCTCTATGACAGCACCACA+AGG - chr7.3:64569865-64569884 MS.gene30261:intron 45.0%
TTTAGGCGGAAAGATCGAAG+GGG + chr7.3:64567498-64567517 None:intergenic 45.0%
! TTGTCCCATTCTAGAGGAGT+TGG - chr7.3:64567812-64567831 MS.gene30261:intron 45.0%
!! ATCCACTGAAGAGGCACTAA+TGG + chr7.3:64568474-64568493 None:intergenic 45.0%
!! CCCTAGAAGCACATTTTAGG+CGG + chr7.3:64567512-64567531 None:intergenic 45.0%
!! CTGGTAGGATTCTAGAACTC+TGG + chr7.3:64569463-64569482 None:intergenic 45.0%
!! GTTAGCTAGTACCGACTTTC+AGG - chr7.3:64568931-64568950 MS.gene30261:intron 45.0%
AAAGATCGAAGGGGCAACGT+GGG + chr7.3:64567489-64567508 None:intergenic 50.0%
AGACCACGTGATAGAGCATG+GGG + chr7.3:64569719-64569738 None:intergenic 50.0%
AGGAGCAGAAATGCTCTGGT+AGG + chr7.3:64569478-64569497 None:intergenic 50.0%
ATTGCCACCGATATCCTCCA+TGG + chr7.3:64569796-64569815 None:intergenic 50.0%
CATCAAATTGGCGTACCCGA+TGG - chr7.3:64568131-64568150 MS.gene30261:intron 50.0%
GACCATTAGTGCCTCTTCAG+TGG - chr7.3:64568469-64568488 MS.gene30261:intron 50.0%
GAGCCAAAATCCCACCATAC+TGG + chr7.3:64569894-64569913 None:intergenic 50.0%
GAGTTGCTCGTTCTCTAGTG+AGG - chr7.3:64568981-64569000 MS.gene30261:intron 50.0%
GATCAGGAGCAGAAATGCTC+TGG + chr7.3:64569482-64569501 None:intergenic 50.0%
GTAACCCATGCAACAGCGTA+GGG - chr7.3:64568057-64568076 MS.gene30261:intron 50.0%
TCTATGACAGCACCACAAGG+CGG - chr7.3:64569868-64569887 MS.gene30261:intron 50.0%
TGAACAAGTCTTGCCGATGG+TGG + chr7.3:64569935-64569954 None:intergenic 50.0%
TGTAACCCATGCAACAGCGT+AGG - chr7.3:64568056-64568075 MS.gene30261:intron 50.0%
! AATTTTTGCGCTTGCCCCCA+TGG - chr7.3:64569813-64569832 MS.gene30261:intron 50.0%
! CAGTTTTACGCCGCAAATCC+AGG + chr7.3:64567602-64567621 None:intergenic 50.0%
AAGATCGAAGGGGCAACGTG+GGG + chr7.3:64567488-64567507 None:intergenic 55.0%
ATTGGCGTACCCGATGGAAG+TGG - chr7.3:64568137-64568156 MS.gene30261:intron 55.0%
CATGGTCCACCATCCACCAT+CGG - chr7.3:64569919-64569938 MS.gene30261:CDS 55.0%
CGAGGGTTGTCCCATTCTAG+AGG - chr7.3:64567806-64567825 MS.gene30261:intron 55.0%
GAAAGATCGAAGGGGCAACG+TGG + chr7.3:64567490-64567509 None:intergenic 55.0%
GTACCCCATGCTCTATCACG+TGG - chr7.3:64569713-64569732 MS.gene30261:CDS 55.0%
TTACATGTCAGAGAGCCCCG+TGG + chr7.3:64568539-64568558 None:intergenic 55.0%
TTGGCCATGGAGGATATCGG+TGG - chr7.3:64569789-64569808 MS.gene30261:CDS 55.0%
!! CAAGTCTTGCCGATGGTGGA+TGG + chr7.3:64569931-64569950 None:intergenic 55.0%
!! CGGCCAGTATGGTGGGATTT+TGG - chr7.3:64569888-64569907 MS.gene30261:intron 55.0%
ACGCTCCCTACGCTGTTGCA+TGG + chr7.3:64568065-64568084 None:intergenic 60.0%
CACAAGGCGGCCAGTATGGT+GGG - chr7.3:64569881-64569900 MS.gene30261:intron 60.0%
CGCTCCCTACGCTGTTGCAT+GGG + chr7.3:64568064-64568083 None:intergenic 60.0%
! CTTCTAGGGGATGCGACCCA+CGG - chr7.3:64567523-64567542 MS.gene30261:CDS 60.0%
! TTCTAGGGGATGCGACCCAC+GGG - chr7.3:64567524-64567543 MS.gene30261:CDS 60.0%
!! CGATGGTGGATGGTGGACCA+TGG + chr7.3:64569921-64569940 None:intergenic 60.0%
!! GTCTTGCCGATGGTGGATGG+TGG + chr7.3:64569928-64569947 None:intergenic 60.0%
!! TGGTGGATGGTGGACCATGG+CGG + chr7.3:64569918-64569937 None:intergenic 60.0%
!!! GGGATTTTGGCTCTCCGCCA+TGG - chr7.3:64569901-64569920 MS.gene30261:intron 60.0%
AAGGGGCAACGTGGGGTCAC+GGG + chr7.3:64567481-64567500 None:intergenic 65.0%
CCACAAGGCGGCCAGTATGG+TGG - chr7.3:64569880-64569899 MS.gene30261:intron 65.0%
CCACCATACTGGCCGCCTTG+TGG + chr7.3:64569883-64569902 None:intergenic 65.0%
GAAGGGGCAACGTGGGGTCA+CGG + chr7.3:64567482-64567501 None:intergenic 65.0%
GCACCACAAGGCGGCCAGTA+TGG - chr7.3:64569877-64569896 MS.gene30261:intron 65.0%
GTTCGCGCTGCTGTGCAACG+AGG - chr7.3:64567561-64567580 MS.gene30261:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 64567471 64570430 64567471 ID=MS.gene30261
chr7.3 mRNA 64567471 64570430 64567471 ID=MS.gene30261.t1;Parent=MS.gene30261
chr7.3 exon 64569915 64570430 64569915 ID=MS.gene30261.t1.exon1;Parent=MS.gene30261.t1
chr7.3 CDS 64569915 64570430 64569915 ID=cds.MS.gene30261.t1;Parent=MS.gene30261.t1
chr7.3 exon 64569688 64569828 64569688 ID=MS.gene30261.t1.exon2;Parent=MS.gene30261.t1
chr7.3 CDS 64569688 64569828 64569688 ID=cds.MS.gene30261.t1;Parent=MS.gene30261.t1
chr7.3 exon 64567471 64567590 64567471 ID=MS.gene30261.t1.exon3;Parent=MS.gene30261.t1
chr7.3 CDS 64567471 64567590 64567471 ID=cds.MS.gene30261.t1;Parent=MS.gene30261.t1
Gene Sequence

>MS.gene30261

ATGATTGCCCGTGACCCCACGTTGCCCCTTCGATCTTTCCGCCTAAAATGTGCTTCTAGGGGATGCGACCCACGGGATATCGATAGATTTGTTCGCGCTGCTGTGCAACGAGGAACTCAGAATTTCATCCTGGATTTGCGGCGTAAAACTGATGAAGAATTTCCAACCGAAAACAAATTAGGTCCTACAATTTCCACAATTTTCAATTCTAGAAACCTTGTGGTTCTTAAGTTGAAAGCATTACTAGTGAATGTTCTTCCTCAAATCGATTTTCCCCTACTCAAAACTCTCCATTTGGGAAGCGTTGCAATTCTAAAAGATTTTGATAAGCTTATCGAGGGTTGTCCCATTCTAGAGGAGTTGGAAATAACTTCTTTACTATGTATGCTTTTGAAAGATGGTATTGGAGAGTTTAAACACTTGCCCAATTTGGTCAGAGCAAACATTTCTGAATTTGTTCCACGAAATATTCAATTTGCTTGGATTTGCAATGCAAAGTTTTTGCGTTTAGAAGTGGTATGACTATCACTAATCATCACTAGCTAGAGTTACATTAAGTTGAAGCTTATTTTTAATGTTTCTCTTTGTAACCCATGCAACAGCGTAGGGAGCGTCAAGTTCACACTTTTCATAACTTAACTCACATGGAGCTTATATTCACATCAAATTGGCGTACCCGATGGAAGTGGTTGCTAGAAATGCTGAAGAATTGCCCTAAACTTCAAAATCTTACCCTTCACAAGGTTGTATTTATGTCAATGATAATAATTTTTTTTGTAGCTCTACTTTCAACTAATTTAATTTTTTTTCTTTTTATGTGGATTTTGACAGCTTTATGGACATGGCATTTATGAGGACTATTGGAAGGAACCAGAAATTATTCCAAACTGCCTTTCCTCTCAACTAAGAACATGCTCGCTTATAGATTACAAAGGCACGAAGTGTGAGCTTCAGTTTGCAGAGTATGTCTTGAAGAATGCCAACTTATTGCGCACAATGACCATTAGTGCCTCTTCAGTGGATTTAACTTTAAAGCACCAAATGCTAATGGATTTATCTTTGTGTCCACGGGGCTCTCTGACATGTAAACTTTCATTCATTTTATTGAAGAAGGTTAGGACACGTCTACTTTATTTCCTATTTTCTAATGTTAAATTAGATAGATGCACTTTGTTTCTTAGGTCTCTCGGTCATATTTTTAAATAGGTTTTATGATTCTGGCTCCTCCAAACCTTTGTGAAACTTCCTTCCTTGCTGATAATTGTTTTTGTTCTTAGCTATATCCATCCTTATTACTGTCTGGCAGTTGTTCACTAAATACCGATATGATGCAGAACTTGAATCATCATTAATAAGCTCATATTGAAATTTGATTGCATGCATGTTGTTGAAGCTGTAAATATTAACATACAGTACAACAATGCATTAGGTAGTATTTTGAATAAGTGTAGGGAGCTTTTGTTAGCTAGTACCGACTTTCAGGTAAGTTTTATTAGGCGACTAGCCAATTGAGTTGCTCGTTCTCTAGTGAGGTCATCTTGATCTATTTCTAGTCCCTGTGTTTTTTATTACTTGTCACTCTCTATTGATACTATTATTATGAATGAAATGTATTATGGCTCTCTAAGTCAAAAATAAATTTGTCAATTATGTCTTCTCTAATCGACACAAAGGCGTAGATTGTGCACCTACATGAACAGCTAGTTCTATGACTCTATGACATGGTTAAGGTTTGCAATTGACACATGTGTATGTGCCTTATACACTAATGACTTGACATGTTTATCTTGGACTTATGGAAACGAGTGACTGATATCATAGTTTGCAACTACGTAGACTAAGTAGTTGAATGTGAGTGTGCTTTCAATGGAAAATTGATAACGTGGTAATGGAAATCGTAAAATCAAGATAAGAAACTGTGTATTATTTGATGAAAAAGTGATGAGCTCAAGGCTCTACAATTGTGGAAAACGGAAAAGAAAAGATAAGCCAGAGTTCTAGAATCCTACCAGAGCATTTCTGCTCCTGATCCATAGAGAGATTCTCTCCTAACCGACTCAATACTAACTTTCTTTGATGTCTTTTTCCTACTACCTTTCCCTCCTACTTATAACAGATTATCGCTAGAAATCAGGAACTACCCTAACTGATTTTATTCCCTCCCCTTTCTTATATGCTTCTAGAAACTTCTAGAGAATGTGCACATGAGACCAGCATTATTGGACCCTGTTTTATGCTTGTTGTACCCCATGCTCTATCACGTGGTCTTATTTTGTTTTAAAGTTTGAGAGTCAAGTTCAATATTGTGCTATCGAATATTGGCCATGGAGGATATCGGTGGCAATTTTTGCGCTTGCCCCCATGGTAAATATTGGCTGATGTTGTAGGTTTTAATTCTCTATGACAGCACCACAAGGCGGCCAGTATGGTGGGATTTTGGCTCTCCGCCATGGTCCACCATCCACCATCGGCAAGACTTGTTCAATATCCGTCTCATTGCAGTAGCATTACAATTTATTTTTTTTACTAGGAAATTGCAATTTTCAACATGAATGCTTTGCCGTAGAAAATAACCAGGTTCCAATAAGTTCATAGTAATTGCAATTTTCAACATTAAGTAACTTTCAGCAGAAATTAGGTTCATTGAATGCTTTGCCGTAGCAAAATGAATGTTTGACTATAGAAAAAGGAACAACAGCTCAACGAAATAGAGCTATAGGGCAATGACTTGAAATTAAGTTATTCACAAAAGGAAAATTACAATATTTGGAATTGATTGTAGATGCAAGTGAGAACAAGAATGTGTTGATAACTGTTAATTGTGTGTTTATATATTTAAGGACGATAACTCGCTTTCGTTGATTTCTTATGTGGATGCTTGCACATCATTTATGCAGGTTGATGGTGAATTGGCAAGTGAATATGAAATTCAAGCAGAAGCTAAGGAGTCATCGACTTAA
Protein sequence

>MS.gene30261.t1

MIARDPTLPLRSFRLKCASRGCDPRDIDRFVRAAVQRGTQNFILDLRRKTDEEFPTENKLGPTISTIFNSRNLVVLKLKALLVNVLPQIDFPLLKTLHLGSVAILKDFDKLIEGCPILEELEITSLLCMLLKDGIGEFKHLPNLVRANISEFVPRNIQFAWICNAKFLRLEVRRERQVHTFHNLTHMELIFTSNWRTRWKWLLEMLKNCPKLQNLTLHKDDNSLSLISYVDACTSFMQVDGELASEYEIQAEAKESST