AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene30044

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30044.t1 MTR_1g041245 61.168 291 97 7 18 297 101 386 2.97e-115 337
MS.gene30044.t1 MTR_7g034115 58.156 282 101 5 18 289 101 375 1.37e-102 305
MS.gene30044.t1 MTR_7g032380 55.052 287 90 7 18 297 102 356 2.66e-92 278
MS.gene30044.t1 MTR_7g032370 51.903 289 114 8 18 297 80 352 7.64e-85 259
MS.gene30044.t1 MTR_7g033990 48.966 290 115 7 18 289 101 375 4.26e-76 238
MS.gene30044.t1 MTR_7g033915 57.075 212 75 6 93 296 161 364 1.75e-68 217
MS.gene30044.t1 MTR_7g034120 44.781 297 131 10 18 297 28 308 1.11e-65 208
MS.gene30044.t1 MTR_7g098510 40.134 299 153 10 15 297 103 391 2.86e-61 199
MS.gene30044.t1 MTR_7g056433 44.000 300 136 12 18 297 102 389 4.49e-60 196
MS.gene30044.t1 MTR_7g115270 40.323 310 140 9 18 294 102 399 1.48e-55 185
MS.gene30044.t1 MTR_7g098570 41.638 293 141 12 18 292 108 388 3.51e-54 183
MS.gene30044.t1 MTR_5g012840 40.830 289 119 8 18 297 73 318 2.53e-53 177
MS.gene30044.t1 MTR_3g108560 39.661 295 150 12 18 296 106 388 1.28e-51 174
MS.gene30044.t1 MTR_0543s0010 39.519 291 155 11 15 296 171 449 1.58e-51 176
MS.gene30044.t1 MTR_7g098480 39.933 298 151 12 15 296 103 388 2.60e-51 176
MS.gene30044.t1 MTR_3g036250 39.796 294 141 12 15 294 92 363 2.29e-50 170
MS.gene30044.t1 MTR_5g008950 38.644 295 157 11 14 297 84 365 5.09e-50 170
MS.gene30044.t1 MTR_8g085430 41.304 276 86 6 18 285 109 316 4.12e-48 164
MS.gene30044.t1 MTR_7g088260 35.906 298 163 11 16 297 177 462 5.60e-48 166
MS.gene30044.t1 MTR_5g013500 39.869 306 127 14 21 297 88 365 1.61e-46 160
MS.gene30044.t1 MTR_3g082890 39.744 312 145 12 18 297 184 484 1.53e-45 160
MS.gene30044.t1 MTR_3g087260 40.214 281 148 8 28 296 184 456 1.03e-44 157
MS.gene30044.t1 MTR_3g087260 29.255 188 118 6 21 203 89 266 3.38e-13 69.7
MS.gene30044.t1 MTR_0492s0020 38.351 279 151 11 15 284 171 437 1.35e-44 157
MS.gene30044.t1 MTR_5g008920 36.552 290 160 8 19 297 105 381 4.66e-44 154
MS.gene30044.t1 MTR_3g108150 36.213 301 161 11 16 296 97 386 2.07e-42 150
MS.gene30044.t1 MTR_7g056430 38.514 296 133 8 14 291 95 359 4.28e-42 155
MS.gene30044.t1 MTR_7g056420 38.514 296 133 8 14 291 95 359 4.79e-42 154
MS.gene30044.t1 MTR_7g007080 36.860 293 151 9 18 295 104 377 4.42e-41 147
MS.gene30044.t1 MTR_3g109982 40.076 262 132 10 42 290 220 469 6.98e-41 151
MS.gene30044.t1 MTR_3g082890 34.582 347 151 12 18 297 104 441 7.33e-41 147
MS.gene30044.t1 MTR_3g040700 36.822 258 131 10 15 259 130 368 3.25e-38 140
MS.gene30044.t1 MTR_3g107100 33.540 322 164 15 6 297 97 398 1.15e-37 138
MS.gene30044.t1 MTR_7g026500 34.323 303 159 11 18 296 108 394 3.38e-37 136
MS.gene30044.t1 MTR_5g058100 33.445 299 167 11 16 297 101 384 5.45e-37 135
MS.gene30044.t1 MTR_3g107160 30.195 308 177 10 8 297 96 383 6.25e-37 135
MS.gene30044.t1 MTR_7g056020 34.859 284 161 9 21 290 94 367 8.15e-37 139
MS.gene30044.t1 MTR_3g105060 33.218 289 150 9 25 297 112 373 4.10e-36 133
MS.gene30044.t1 MTR_5g007410 33.333 294 164 8 21 294 124 405 5.65e-36 134
MS.gene30044.t1 MTR_3g023630 33.813 278 163 8 25 294 107 371 1.46e-35 132
MS.gene30044.t1 MTR_2g007400 32.476 311 161 12 16 295 109 401 3.77e-35 131
MS.gene30044.t1 MTR_7g081290 50.685 146 62 6 155 297 118 256 7.29e-35 127
MS.gene30044.t1 MTR_5g058000 34.868 304 166 10 6 296 90 374 1.17e-34 129
MS.gene30044.t1 MTR_7g056277 32.982 285 162 10 24 297 362 628 1.66e-34 132
MS.gene30044.t1 MTR_2g020180 49.645 141 54 4 159 297 90 215 1.93e-34 124
MS.gene30044.t1 MTR_1g108800 34.364 291 156 8 21 295 69 340 2.27e-34 128
MS.gene30044.t1 MTR_3g107120 32.872 289 161 10 21 294 118 388 3.75e-34 128
MS.gene30044.t1 MTR_7g075820 33.553 304 144 12 27 295 112 392 5.61e-34 128
MS.gene30044.t1 MTR_2g007450 30.723 332 165 12 9 295 107 418 6.58e-34 128
MS.gene30044.t1 MTR_1g066830 31.908 304 166 11 16 296 101 386 4.85e-33 125
MS.gene30044.t1 MTR_5g073420 32.686 309 169 13 7 297 93 380 6.01e-33 125
MS.gene30044.t1 MTR_5g073370 33.824 272 147 10 39 295 125 378 1.34e-32 124
MS.gene30044.t1 MTR_4g080610 32.646 291 147 11 21 296 100 356 1.36e-32 123
MS.gene30044.t1 MTR_7g056287 31.930 285 165 11 24 297 106 372 2.54e-32 123
MS.gene30044.t1 MTR_6g022500 32.877 292 170 10 19 297 198 476 2.56e-32 124
MS.gene30044.t1 MTR_1g108820 32.895 304 158 11 20 295 99 384 1.02e-31 121
MS.gene30044.t1 MTR_2g005990 34.416 308 153 15 18 295 105 393 1.72e-31 121
MS.gene30044.t1 MTR_3g467400 34.084 311 139 14 16 297 94 367 2.31e-31 120
MS.gene30044.t1 MTR_1g107385 33.094 278 142 11 41 295 165 421 2.44e-31 121
MS.gene30044.t1 MTR_6g040270 32.095 296 169 11 19 297 101 381 6.32e-31 119
MS.gene30044.t1 MTR_1g085120 30.640 297 176 10 18 297 188 471 7.92e-31 120
MS.gene30044.t1 MTR_1g085120 30.640 297 176 10 18 297 263 546 1.51e-30 120
MS.gene30044.t1 MTR_6g045333 31.833 311 161 13 16 296 156 445 3.62e-30 118
MS.gene30044.t1 MTR_7g034110 32.975 279 134 9 18 287 98 332 4.60e-30 116
MS.gene30044.t1 MTR_1g053025 28.165 316 176 11 8 294 93 386 4.96e-30 117
MS.gene30044.t1 MTR_5g083890 35.573 253 124 11 38 265 136 374 1.47e-29 117
MS.gene30044.t1 MTR_1089s0010 30.847 295 167 10 20 297 105 379 2.10e-28 112
MS.gene30044.t1 MTR_2g007360 31.908 304 161 15 24 295 83 372 2.76e-28 112
MS.gene30044.t1 MTR_5g054920 37.056 197 104 7 95 279 3 191 8.47e-28 106
MS.gene30044.t1 MTR_6g022520 31.818 308 175 10 10 297 21 313 1.57e-26 106
MS.gene30044.t1 MTR_7g114710 31.724 290 145 12 25 297 111 364 1.96e-26 107
MS.gene30044.t1 MTR_5g039470 31.660 259 148 9 21 261 80 327 3.93e-26 105
MS.gene30044.t1 MTR_2g036470 32.000 300 158 11 21 294 114 393 4.49e-26 106
MS.gene30044.t1 MTR_6g029350 36.493 211 109 10 98 297 2 198 5.36e-26 103
MS.gene30044.t1 MTR_3g115430 47.541 122 54 5 178 297 12 125 8.67e-26 99.4
MS.gene30044.t1 MTR_3g071850 29.766 299 173 11 18 293 121 405 8.87e-26 107
MS.gene30044.t1 MTR_3g099830 32.143 280 141 12 41 295 117 372 9.11e-26 105
MS.gene30044.t1 MTR_4g039440 31.561 301 167 11 21 297 127 412 2.34e-25 104
MS.gene30044.t1 MTR_7g007340 33.083 266 142 11 41 291 107 351 2.46e-25 103
MS.gene30044.t1 MTR_7g083740 29.236 301 163 12 18 296 94 366 2.50e-25 103
MS.gene30044.t1 MTR_7g060470 30.000 300 162 12 22 290 105 387 3.24e-25 103
MS.gene30044.t1 MTR_6g011460 30.164 305 163 11 18 297 142 421 1.95e-24 102
MS.gene30044.t1 MTR_5g039450 29.592 294 148 10 21 294 101 355 2.05e-24 101
MS.gene30044.t1 MTR_2g008310 31.915 282 137 13 42 297 119 371 3.03e-24 101
MS.gene30044.t1 MTR_5g045170 30.897 301 162 11 21 295 109 389 3.09e-24 101
MS.gene30044.t1 MTR_3g108560 36.500 200 105 9 18 203 106 297 6.35e-24 99.4
MS.gene30044.t1 MTR_6g022360 28.767 292 179 10 21 296 129 407 1.22e-23 100
MS.gene30044.t1 MTR_0009s0080 31.047 277 135 11 20 265 116 367 1.42e-23 100
MS.gene30044.t1 MTR_5g031970 30.282 284 160 11 21 294 98 353 1.80e-23 99.0
MS.gene30044.t1 MTR_2g008320 30.605 281 144 12 42 297 173 427 3.10e-23 99.4
MS.gene30044.t1 MTR_2g007090 33.333 240 116 11 68 296 87 293 8.55e-23 95.9
MS.gene30044.t1 MTR_7g055943 30.435 299 176 11 18 297 136 421 1.21e-22 97.4
MS.gene30044.t1 MTR_3g107940 42.969 128 61 3 163 289 89 205 6.51e-22 94.4
MS.gene30044.t1 MTR_5g063950 29.720 286 178 8 22 294 84 359 1.38e-21 93.6
MS.gene30044.t1 MTR_7g075800 28.882 322 152 15 18 277 101 407 4.23e-21 93.6
MS.gene30044.t1 MTR_3g095630 34.524 168 96 6 125 287 17 175 4.90e-21 88.6
MS.gene30044.t1 MTR_2g007610 38.816 152 79 5 145 295 11 149 7.35e-21 87.8
MS.gene30044.t1 MTR_6g044572 26.948 308 170 10 11 297 115 388 1.03e-20 91.7
MS.gene30044.t1 MTR_3g026920 31.071 280 127 13 41 294 163 402 1.15e-20 91.7
MS.gene30044.t1 MTR_1g043170 52.439 82 35 2 217 297 10 88 1.23e-20 84.7
MS.gene30044.t1 MTR_6g044560 27.362 307 168 11 12 297 292 564 5.17e-20 90.5
MS.gene30044.t1 MTR_4g098590 31.071 280 154 11 21 275 99 364 9.15e-20 88.6
MS.gene30044.t1 MTR_2g007550 34.074 135 73 2 160 294 214 332 2.98e-19 87.0
MS.gene30044.t1 MTR_7g021990 30.000 280 158 9 42 297 164 429 5.87e-19 86.7
MS.gene30044.t1 MTR_7g021950 30.000 280 158 9 42 297 164 429 7.45e-19 86.7
MS.gene30044.t1 MTR_7g056280 32.432 185 103 6 98 265 367 546 9.23e-19 86.7
MS.gene30044.t1 MTR_3g094980 45.238 84 45 1 183 265 34 117 1.90e-18 81.3
MS.gene30044.t1 MTR_2g007500 30.224 268 141 12 8 244 82 334 2.38e-18 84.3
MS.gene30044.t1 MTR_7g034090 32.161 199 116 8 101 297 131 312 3.61e-18 84.3
MS.gene30044.t1 MTR_3g113770 27.799 259 112 9 9 258 145 337 1.27e-17 82.0
MS.gene30044.t1 MTR_3g106960 37.580 157 83 7 137 289 45 190 1.56e-17 80.5
MS.gene30044.t1 MTR_8g077460 28.731 268 158 10 36 297 135 375 3.75e-17 81.3
MS.gene30044.t1 MTR_4g096730 47.619 84 40 2 215 297 9 89 7.46e-17 74.7
MS.gene30044.t1 MTR_1377s0010 34.351 131 75 4 137 265 6 127 7.98e-17 75.9
MS.gene30044.t1 MTR_7g007370 31.022 274 149 9 34 293 86 333 3.25e-16 78.2
MS.gene30044.t1 MTR_5g008910 31.646 237 123 10 92 297 74 302 4.40e-16 77.4
MS.gene30044.t1 MTR_3g110010 34.078 179 98 7 42 209 220 389 9.83e-16 77.8
MS.gene30044.t1 MTR_5g024060 34.058 138 79 5 155 290 74 201 1.05e-15 75.5
MS.gene30044.t1 MTR_1g108820 30.952 210 109 8 20 203 99 298 1.68e-15 76.3
MS.gene30044.t1 MTR_5g073340 29.339 242 141 8 12 234 70 300 1.92e-15 75.5
MS.gene30044.t1 MTR_1g059860 25.614 285 151 8 21 258 41 311 2.80e-15 75.5
MS.gene30044.t1 MTR_3g113785 43.137 102 42 3 163 258 4 95 4.29e-15 70.1
MS.gene30044.t1 MTR_5g039490 28.767 292 155 10 21 296 121 375 7.16e-15 74.7
MS.gene30044.t1 MTR_3g108140 46.250 80 42 1 218 296 68 147 9.14e-15 71.2
MS.gene30044.t1 MTR_7g022105 28.626 262 149 9 42 279 165 412 1.18e-14 74.3
MS.gene30044.t1 MTR_8g014210 35.780 109 65 1 189 297 128 231 1.76e-13 68.9
MS.gene30044.t1 MTR_5g033200 29.932 147 91 4 125 268 12 149 4.68e-13 66.6
MS.gene30044.t1 MTR_3g071790 28.022 182 104 8 96 258 28 201 2.73e-12 65.1
MS.gene30044.t1 MTR_8g062310 26.100 341 169 16 16 297 112 428 5.14e-12 66.2
MS.gene30044.t1 MTR_5g076150 30.769 221 106 11 42 230 22 227 1.09e-11 63.9
MS.gene30044.t1 MTR_8g467800 27.236 246 132 9 67 297 240 453 1.68e-11 64.7
MS.gene30044.t1 MTR_8g016130 36.719 128 69 5 7 130 43 162 1.89e-11 61.6
MS.gene30044.t1 MTR_5g092960 26.715 277 164 10 34 296 210 461 5.71e-11 63.2
MS.gene30044.t1 MTR_2g007530 33.094 139 63 5 156 294 226 334 6.59e-11 62.8
MS.gene30044.t1 MTR_7g022060 35.955 89 56 1 204 291 236 324 6.82e-11 62.4
MS.gene30044.t1 MTR_7g021930 35.870 92 58 1 204 294 236 327 7.62e-11 62.4
MS.gene30044.t1 MTR_7g021970 35.870 92 58 1 204 294 236 327 7.62e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30044.t1 AT1G32375 27.793 367 183 15 1 297 68 422 1.70e-17 82.4
MS.gene30044.t1 AT1G32375 27.793 367 183 15 1 297 68 422 1.70e-17 82.4
MS.gene30044.t1 AT3G54910 27.742 310 153 11 42 297 81 373 1.23e-14 73.9
MS.gene30044.t1 AT3G54910 27.742 310 153 11 42 297 81 373 1.23e-14 73.9
MS.gene30044.t1 AT3G54910 27.742 310 153 11 42 297 81 373 1.23e-14 73.9
MS.gene30044.t1 AT4G26340 32.353 136 87 2 163 297 288 419 5.14e-13 69.3

Find 60 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGAAGGTTAGGACAATTT+TGG 0.205184 7.1:+62667266 None:intergenic
GAATATTTGCTCTCACTAAT+TGG 0.238032 7.1:+62667572 None:intergenic
AGCTGAGTTGAAAGACATTC+TGG 0.253372 7.1:+62667066 None:intergenic
TTTCCGGATGAAGAGTTTAA+TGG 0.304681 7.1:-62667601 MS.gene30044:CDS
ATTGCTCAAAACACTTCATT+TGG 0.307784 7.1:-62667740 MS.gene30044:CDS
GCATGCAACAAGTTTATTCC+TGG 0.353922 7.1:-62667629 MS.gene30044:CDS
CAGAATCTCAACCTTGAGAT+AGG 0.354199 7.1:-62667862 MS.gene30044:CDS
AATTTGACTCACATGGAGAT+TGG 0.383846 7.1:-62667343 MS.gene30044:CDS
GAAAGACATTCTGGAACAAT+TGG 0.392140 7.1:+62667075 None:intergenic
TCTGGTTGAAGTGGTAGATC+TGG 0.392987 7.1:-62668397 MS.gene30044:CDS
TCAATCTCGTTCCGCCCATC+TGG 0.407164 7.1:-62667715 MS.gene30044:CDS
AAAGTTTCTATCCACAAAAC+TGG 0.410682 7.1:-62667518 MS.gene30044:intron
TTCATTCAATCCCAATGGCT+TGG 0.413718 7.1:-62667552 MS.gene30044:CDS
TCATTCAATCCCAATGGCTT+GGG 0.415899 7.1:-62667551 MS.gene30044:CDS
TGAAATTATCTTTGTGTCCA+AGG 0.436414 7.1:-62666900 MS.gene30044:CDS
AAGATAATTTCATTAGCATC+TGG 0.437146 7.1:+62666911 None:intergenic
ATGGTAAGATTCTGAAGGTT+AGG 0.437422 7.1:+62667256 None:intergenic
CTTTCAAAATTTGACTCACA+TGG 0.440042 7.1:-62667350 MS.gene30044:CDS
GCATGCAAATCCTCTAAAAC+AGG 0.450161 7.1:+62667646 None:intergenic
CATGCAACAAGTTTATTCCT+GGG 0.458540 7.1:-62667628 MS.gene30044:CDS
GCATTACGAACCCAAGCCAT+TGG 0.459558 7.1:+62667541 None:intergenic
GGGAAAGAACATCATGCATC+TGG 0.469832 7.1:-62668415 None:intergenic
CATTACGAACCCAAGCCATT+GGG 0.477262 7.1:+62667542 None:intergenic
GAAATTATCTTTGTGTCCAA+GGG 0.482391 7.1:-62666899 MS.gene30044:CDS
AGCTTTATATTTAAATCCAC+CGG 0.482711 7.1:+62666934 None:intergenic
AATTGAGATATGATTGGCTT+GGG 0.493411 7.1:-62667315 MS.gene30044:CDS
ATTGGCTTGGGAAGTGGAAG+TGG 0.495880 7.1:-62667303 MS.gene30044:CDS
GATTTGAATTGAGATATGAT+TGG 0.496357 7.1:-62667321 MS.gene30044:CDS
GATATGATTGGCTTGGGAAG+TGG 0.498477 7.1:-62667309 MS.gene30044:CDS
AGCAATGGAAAATTGACCTC+AGG 0.498844 7.1:+62667757 None:intergenic
AAGCCATTAAACTCTTCATC+CGG 0.525720 7.1:+62667598 None:intergenic
CTGGTGGTGCTCATAGCCAT+AGG 0.531014 7.1:-62667696 MS.gene30044:CDS
CTGGTTGAAGTGGTAGATCT+GGG 0.533194 7.1:-62668396 MS.gene30044:CDS
GGCTATGAGCACCACCAGAT+GGG 0.536823 7.1:+62667701 None:intergenic
TTGACTATGACTATGACGAT+TGG 0.542689 7.1:-62667107 MS.gene30044:CDS
GAATTGAGATATGATTGGCT+TGG 0.544799 7.1:-62667316 MS.gene30044:CDS
CATGAAAATTAGTGCCTCAC+CGG 0.549627 7.1:-62666953 MS.gene30044:CDS
AGACATTCTGGAACAATTGG+TGG 0.549708 7.1:+62667078 None:intergenic
CTTCATCCGGAAAACGACCC+AGG 0.556064 7.1:+62667611 None:intergenic
CATCATGCATCTGGTTGAAG+TGG 0.556199 7.1:-62668406 None:intergenic
ATCTCGTTCCGCCCATCTGG+TGG 0.556483 7.1:-62667712 MS.gene30044:CDS
CTATGACTATGACGATTGGA+TGG 0.560911 7.1:-62667103 MS.gene30044:CDS
TGGCTATGAGCACCACCAGA+TGG 0.569185 7.1:+62667700 None:intergenic
AACAAATAAGACCTCGCGAA+TGG 0.570158 7.1:+62667237 None:intergenic
AAATATTCATTCAATCCCAA+TGG 0.576301 7.1:-62667557 MS.gene30044:CDS
GAAAATTAGTGCCTCACCGG+TGG 0.580886 7.1:-62666950 MS.gene30044:CDS
ATCCTCTAAAACAGGACACC+CGG 0.590014 7.1:+62667654 None:intergenic
TCAAATTCATTCTCATAACT+AGG 0.594416 7.1:+62667126 None:intergenic
TTACATGTAGTAGAGCCCCT+TGG 0.603968 7.1:+62666883 None:intergenic
ACTTAAAGAAACCTATCTCA+AGG 0.608637 7.1:+62667851 None:intergenic
GAGGCGCTCATATGACTACC+AGG 0.612385 7.1:-62667380 MS.gene30044:CDS
CGCGAATGGTAAGATTCTGA+AGG 0.623391 7.1:+62667251 None:intergenic
GACATTCTGGAACAATTGGT+GGG 0.625033 7.1:+62667079 None:intergenic
TATATTTAAATCCACCGGTG+AGG 0.637294 7.1:+62666939 None:intergenic
TCAGAATCTTACCATTCGCG+AGG 0.653321 7.1:-62667248 MS.gene30044:intron
ATTAATGCTGCTATGCAACG+TGG 0.669305 7.1:-62667889 MS.gene30044:CDS
AAATTATCTTTGTGTCCAAG+GGG 0.673883 7.1:-62666898 MS.gene30044:CDS
TATGAGCACCACCAGATGGG+CGG 0.679580 7.1:+62667704 None:intergenic
GAAAATACACGATCTTCCTG+AGG 0.701166 7.1:-62667773 MS.gene30044:CDS
ACATCAACTTAAGAACAGTG+AGG 0.774936 7.1:+62667800 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTAGGTAAATTAATTTTT+TGG - chr7.1:62666951-62666970 MS.gene30044:CDS 10.0%
!! AGAAAAAATAATCAAAATGA+TGG + chr7.1:62668094-62668113 None:intergenic 15.0%
!! TATCATGTTAAAAACAAATT+TGG - chr7.1:62667791-62667810 MS.gene30044:CDS 15.0%
!! ATAAATCTATTAACATCATG+AGG + chr7.1:62667362-62667381 None:intergenic 20.0%
!! ATGTTAAAAACAAATTTGGT+TGG - chr7.1:62667795-62667814 MS.gene30044:CDS 20.0%
!! CCAATAAATTATGGATAATT+AGG - chr7.1:62666935-62666954 MS.gene30044:CDS 20.0%
!! CCTAATTATCCATAATTTAT+TGG + chr7.1:62666938-62666957 None:intergenic 20.0%
!!! AAAATTGTTTGTTGTTTGAT+AGG - chr7.1:62668118-62668137 MS.gene30044:intron 20.0%
!!! AATTTTTTGGTACAAAAGTT+TGG - chr7.1:62667832-62667851 MS.gene30044:CDS 20.0%
!!! TTTTAACATGATAAACCATT+GGG + chr7.1:62667784-62667803 None:intergenic 20.0%
!!! TTTTTAACATGATAAACCAT+TGG + chr7.1:62667785-62667804 None:intergenic 20.0%
! AAATATTCATTCAATCCCAA+TGG - chr7.1:62667712-62667731 MS.gene30044:CDS 25.0%
! AAGATAATTTCATTAGCATC+TGG + chr7.1:62668361-62668380 None:intergenic 25.0%
! GATTTGAATTGAGATATGAT+TGG - chr7.1:62667948-62667967 MS.gene30044:intron 25.0%
! GTAGGAAATCCAATAAATTA+TGG - chr7.1:62666926-62666945 MS.gene30044:CDS 25.0%
! TCAAATTCATTCTCATAACT+AGG + chr7.1:62668146-62668165 None:intergenic 25.0%
! TTTAACATGATAAACCATTG+GGG + chr7.1:62667783-62667802 None:intergenic 25.0%
!! AGCTTTATATTTAAATCCAC+CGG + chr7.1:62668338-62668357 None:intergenic 25.0%
AAAGTTTCTATCCACAAAAC+TGG - chr7.1:62667751-62667770 MS.gene30044:CDS 30.0%
AAATTATCTTTGTGTCCAAG+GGG - chr7.1:62668371-62668390 MS.gene30044:CDS 30.0%
ACTTAAAGAAACCTATCTCA+AGG + chr7.1:62667421-62667440 None:intergenic 30.0%
ATTGCTCAAAACACTTCATT+TGG - chr7.1:62667529-62667548 MS.gene30044:CDS 30.0%
CTTTCAAAATTTGACTCACA+TGG - chr7.1:62667919-62667938 MS.gene30044:intron 30.0%
GAAATTATCTTTGTGTCCAA+GGG - chr7.1:62668370-62668389 MS.gene30044:CDS 30.0%
GAATATTTGCTCTCACTAAT+TGG + chr7.1:62667700-62667719 None:intergenic 30.0%
GTTAATGCAACTAAAAACAC+AGG + chr7.1:62667450-62667469 None:intergenic 30.0%
TGAAATTATCTTTGTGTCCA+AGG - chr7.1:62668369-62668388 MS.gene30044:CDS 30.0%
! CATGTGAGTCAAATTTTGAA+AGG + chr7.1:62667921-62667940 None:intergenic 30.0%
!! AAATGAAGTGTTTTGAGCAA+TGG + chr7.1:62667530-62667549 None:intergenic 30.0%
!! AATTGAGATATGATTGGCTT+GGG - chr7.1:62667954-62667973 MS.gene30044:intron 30.0%
!! ATAGCCATAGGTGTTTTAAA+AGG - chr7.1:62667585-62667604 MS.gene30044:CDS 30.0%
!! TGAGTCAAATTTTGAAAGGT+GGG + chr7.1:62667917-62667936 None:intergenic 30.0%
AAGCCATTAAACTCTTCATC+CGG + chr7.1:62667674-62667693 None:intergenic 35.0%
AATTTGACTCACATGGAGAT+TGG - chr7.1:62667926-62667945 MS.gene30044:intron 35.0%
ACATCAACTTAAGAACAGTG+AGG + chr7.1:62667472-62667491 None:intergenic 35.0%
ATGCAACTAAAAACACAGGT+AGG + chr7.1:62667446-62667465 None:intergenic 35.0%
CATGCAACAAGTTTATTCCT+GGG - chr7.1:62667641-62667660 MS.gene30044:CDS 35.0%
TATATTTAAATCCACCGGTG+AGG + chr7.1:62668333-62668352 None:intergenic 35.0%
TTGACTATGACTATGACGAT+TGG - chr7.1:62668162-62668181 MS.gene30044:intron 35.0%
TTTCCGGATGAAGAGTTTAA+TGG - chr7.1:62667668-62667687 MS.gene30044:CDS 35.0%
! ATGGTAAGATTCTGAAGGTT+AGG + chr7.1:62668016-62668035 None:intergenic 35.0%
! GAATTGAGATATGATTGGCT+TGG - chr7.1:62667953-62667972 MS.gene30044:intron 35.0%
! TCTGAAGGTTAGGACAATTT+TGG + chr7.1:62668006-62668025 None:intergenic 35.0%
! TTTGAAGTGATTCACGGTTA+GGG - chr7.1:62667013-62667032 MS.gene30044:CDS 35.0%
!! GAAAGACATTCTGGAACAAT+TGG + chr7.1:62668197-62668216 None:intergenic 35.0%
!! GTGAGTCAAATTTTGAAAGG+TGG + chr7.1:62667918-62667937 None:intergenic 35.0%
!!! GAAGCCTTTTAAAACACCTA+TGG + chr7.1:62667592-62667611 None:intergenic 35.0%
!!! TGTTTTAAAAGGCTTCTCTC+CGG - chr7.1:62667596-62667615 MS.gene30044:CDS 35.0%
AAAACTGGTATGAACCCCAA+TGG - chr7.1:62667766-62667785 MS.gene30044:CDS 40.0%
AACAAATAAGACCTCGCGAA+TGG + chr7.1:62668035-62668054 None:intergenic 40.0%
AATTAGAGCTTCTACGGCAA+AGG + chr7.1:62666987-62667006 None:intergenic 40.0%
ACATCATGAGGATTGAAACC+AGG + chr7.1:62667350-62667369 None:intergenic 40.0%
AGCAATGGAAAATTGACCTC+AGG + chr7.1:62667515-62667534 None:intergenic 40.0%
ATTAATGCTGCTATGCAACG+TGG - chr7.1:62667380-62667399 MS.gene30044:CDS 40.0%
CAGAATCTCAACCTTGAGAT+AGG - chr7.1:62667407-62667426 MS.gene30044:intron 40.0%
CATGAAAATTAGTGCCTCAC+CGG - chr7.1:62668316-62668335 MS.gene30044:intron 40.0%
CCAAAAGATGGAAACCATTG+TGG - chr7.1:62667183-62667202 MS.gene30044:intron 40.0%
CTATGACTATGACGATTGGA+TGG - chr7.1:62668166-62668185 MS.gene30044:intron 40.0%
GAAAATACACGATCTTCCTG+AGG - chr7.1:62667496-62667515 MS.gene30044:intron 40.0%
GAAGAAAGCTGTTCATGTGT+AGG - chr7.1:62666908-62666927 MS.gene30044:CDS 40.0%
GCATGCAACAAGTTTATTCC+TGG - chr7.1:62667640-62667659 MS.gene30044:CDS 40.0%
GCCAGAAATTAGAGCTTCTA+CGG + chr7.1:62666993-62667012 None:intergenic 40.0%
TAGAGAGAAAAGAGAGGATG+TGG + chr7.1:62667133-62667152 None:intergenic 40.0%
TCATTCAATCCCAATGGCTT+GGG - chr7.1:62667718-62667737 MS.gene30044:CDS 40.0%
TTCATTCAATCCCAATGGCT+TGG - chr7.1:62667717-62667736 MS.gene30044:CDS 40.0%
! AGCTGAGTTGAAAGACATTC+TGG + chr7.1:62668206-62668225 None:intergenic 40.0%
! CCACAATGGTTTCCATCTTT+TGG + chr7.1:62667186-62667205 None:intergenic 40.0%
! TGCTCGCTGATAGCTTTTAA+AGG - chr7.1:62668233-62668252 MS.gene30044:intron 40.0%
! TGCTTTTGCAACACATGTAG+AGG - chr7.1:62667870-62667889 MS.gene30044:CDS 40.0%
!! AATTTTGAAAGGTGGGAACC+TGG + chr7.1:62667910-62667929 None:intergenic 40.0%
!! AGACATTCTGGAACAATTGG+TGG + chr7.1:62668194-62668213 None:intergenic 40.0%
!! ATGGTTTCCATCTTTTGGAG+AGG + chr7.1:62667181-62667200 None:intergenic 40.0%
!! CTTTGAAGTGATTCACGGTT+AGG - chr7.1:62667012-62667031 MS.gene30044:CDS 40.0%
!! GACATTCTGGAACAATTGGT+GGG + chr7.1:62668193-62668212 None:intergenic 40.0%
!! GCATGCAAATCCTCTAAAAC+AGG + chr7.1:62667626-62667645 None:intergenic 40.0%
!! TCTGGCTTTGAAGTGATTCA+CGG - chr7.1:62667007-62667026 MS.gene30044:CDS 40.0%
!! TTTATTCCTGGGTCGTTTTC+CGG - chr7.1:62667652-62667671 MS.gene30044:CDS 40.0%
!!! GTTTTAAAAGGCTTCTCTCC+GGG - chr7.1:62667597-62667616 MS.gene30044:CDS 40.0%
AGAGAGGATGTGGCAGATAA+CGG + chr7.1:62667123-62667142 None:intergenic 45.0%
CATTACGAACCCAAGCCATT+GGG + chr7.1:62667730-62667749 None:intergenic 45.0%
CGCGAATGGTAAGATTCTGA+AGG + chr7.1:62668021-62668040 None:intergenic 45.0%
CTGGTTGAAGTGGTAGATCT+GGG - chr7.1:62666873-62666892 MS.gene30044:CDS 45.0%
GCTTGGTAGAGAGAAAAGAG+AGG + chr7.1:62667139-62667158 None:intergenic 45.0%
TCAGAATCTTACCATTCGCG+AGG - chr7.1:62668021-62668040 MS.gene30044:intron 45.0%
TCTGGTTGAAGTGGTAGATC+TGG - chr7.1:62666872-62666891 MS.gene30044:CDS 45.0%
TTACATGTAGTAGAGCCCCT+TGG + chr7.1:62668389-62668408 None:intergenic 45.0%
! GCCGTAGAAGCTCTAATTTC+TGG - chr7.1:62666989-62667008 MS.gene30044:CDS 45.0%
! TGGGGTTCATACCAGTTTTG+TGG + chr7.1:62667765-62667784 None:intergenic 45.0%
!! ATCCTCTAAAACAGGACACC+CGG + chr7.1:62667618-62667637 None:intergenic 45.0%
!! CGGTGAGGCACTAATTTTCA+TGG + chr7.1:62668318-62668337 None:intergenic 45.0%
!! GATATGATTGGCTTGGGAAG+TGG - chr7.1:62667960-62667979 MS.gene30044:intron 45.0%
!! GCGCTGATTCTGTCTAATGT+TGG + chr7.1:62667089-62667108 None:intergenic 45.0%
!! GTATAATTAAAAAAAATTAT+CGG + chr7.1:62668069-62668088 None:intergenic 5.0%
!!! TTCTTTTATTTTTAATTTTT+TGG - chr7.1:62667819-62667838 MS.gene30044:CDS 5.0%
ATCGTTCCGCCTCAAAAGTC+GGG - chr7.1:62667316-62667335 MS.gene30044:CDS 50.0%
CGAGCATCCTCTCCAAAAGA+TGG - chr7.1:62667171-62667190 MS.gene30044:intron 50.0%
GAAAATTAGTGCCTCACCGG+TGG - chr7.1:62668319-62668338 MS.gene30044:intron 50.0%
GATCGTTCCGCCTCAAAAGT+CGG - chr7.1:62667315-62667334 MS.gene30044:CDS 50.0%
GCATTACGAACCCAAGCCAT+TGG + chr7.1:62667731-62667750 None:intergenic 50.0%
GTAGAAACTGCGAGCCACAA+TGG + chr7.1:62667200-62667219 None:intergenic 50.0%
TTTGGAGAGGATGCTCGTTG+CGG + chr7.1:62667168-62667187 None:intergenic 50.0%
!! ATGTGGCAGATAACGGAGTC+CGG + chr7.1:62667116-62667135 None:intergenic 50.0%
!! ATTGGCTTGGGAAGTGGAAG+TGG - chr7.1:62667966-62667985 MS.gene30044:intron 50.0%
!!! GACTTTTGAGGCGGAACGAT+CGG + chr7.1:62667316-62667335 None:intergenic 50.0%
AACGATCGGATAGGCAGGGT+GGG + chr7.1:62667302-62667321 None:intergenic 55.0%
AGACAGAATCAGCGCCCTTC+CGG - chr7.1:62667094-62667113 MS.gene30044:CDS 55.0%
CTTCATCCGGAAAACGACCC+AGG + chr7.1:62667661-62667680 None:intergenic 55.0%
GGCTATGAGCACCACCAGAT+GGG + chr7.1:62667571-62667590 None:intergenic 55.0%
TATGAGCACCACCAGATGGG+CGG + chr7.1:62667568-62667587 None:intergenic 55.0%
TCAATCTCGTTCCGCCCATC+TGG - chr7.1:62667554-62667573 MS.gene30044:CDS 55.0%
TCGTTCCGCCTCAAAAGTCG+GGG - chr7.1:62667317-62667336 MS.gene30044:CDS 55.0%
TGGCTATGAGCACCACCAGA+TGG + chr7.1:62667572-62667591 None:intergenic 55.0%
TTGAGGCGGAACGATCGGAT+AGG + chr7.1:62667311-62667330 None:intergenic 55.0%
! AAAAGTCGGGGCATCTCTCC+TGG - chr7.1:62667329-62667348 MS.gene30044:CDS 55.0%
! CTGGTGGTGCTCATAGCCAT+AGG - chr7.1:62667573-62667592 MS.gene30044:CDS 55.0%
! GAGAGATGCCCCGACTTTTG+AGG + chr7.1:62667328-62667347 None:intergenic 55.0%
! GAGGCGCTCATATGACTACC+AGG - chr7.1:62667889-62667908 MS.gene30044:CDS 55.0%
!! AGATGCCCCGACTTTTGAGG+CGG + chr7.1:62667325-62667344 None:intergenic 55.0%
!! CTCCGGGTGTCCTGTTTTAG+AGG - chr7.1:62667613-62667632 MS.gene30044:CDS 55.0%
!! GCAGATAACGGAGTCCGGAA+GGG + chr7.1:62667111-62667130 None:intergenic 55.0%
ACGATCGGATAGGCAGGGTG+GGG + chr7.1:62667301-62667320 None:intergenic 60.0%
ATCTCGTTCCGCCCATCTGG+TGG - chr7.1:62667557-62667576 MS.gene30044:CDS 60.0%
GAACGATCGGATAGGCAGGG+TGG + chr7.1:62667303-62667322 None:intergenic 60.0%
GCGGAACGATCGGATAGGCA+GGG + chr7.1:62667306-62667325 None:intergenic 60.0%
GCTCGTTGCGGCAGATTGCT+TGG + chr7.1:62667156-62667175 None:intergenic 60.0%
!! GGCAGATAACGGAGTCCGGA+AGG + chr7.1:62667112-62667131 None:intergenic 60.0%
GATAGGCAGGGTGGGGTCAC+GGG + chr7.1:62667294-62667313 None:intergenic 65.0%
GGATAGGCAGGGTGGGGTCA+CGG + chr7.1:62667295-62667314 None:intergenic 65.0%
GGCGGAACGATCGGATAGGC+AGG + chr7.1:62667307-62667326 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 62666867 62668424 62666867 ID=MS.gene30044
chr7.1 mRNA 62666867 62668424 62666867 ID=MS.gene30044.t1;Parent=MS.gene30044
chr7.1 exon 62668366 62668424 62668366 ID=MS.gene30044.t1.exon1;Parent=MS.gene30044.t1
chr7.1 CDS 62668366 62668424 62668366 ID=cds.MS.gene30044.t1;Parent=MS.gene30044.t1
chr7.1 exon 62667519 62667915 62667519 ID=MS.gene30044.t1.exon2;Parent=MS.gene30044.t1
chr7.1 CDS 62667519 62667915 62667519 ID=cds.MS.gene30044.t1;Parent=MS.gene30044.t1
chr7.1 exon 62667249 62667401 62667249 ID=MS.gene30044.t1.exon3;Parent=MS.gene30044.t1
chr7.1 CDS 62667249 62667401 62667249 ID=cds.MS.gene30044.t1;Parent=MS.gene30044.t1
chr7.1 exon 62666867 62667151 62666867 ID=MS.gene30044.t1.exon4;Parent=MS.gene30044.t1
chr7.1 CDS 62666867 62667151 62666867 ID=cds.MS.gene30044.t1;Parent=MS.gene30044.t1
Gene Sequence

>MS.gene30044

ATGCATCTGGTTGAAGTGGTAGATCTGGGTCATGTGAAGAAGAAGAAAGCTGTTCATGTGTAGGAAATCCAATAAATTATGGATAATTAGGTAAATTAATTTTTTGGATTCGATTTTCCTTTGCCGTAGAAGCTCTAATTTCTGGCTTTGAAGTGATTCACGGTTAGGGCTAAAATTCGATTTTGCTATCTATCAAAGATGACTTCTCAGCGTTCAATTCCAACATTAGACAGAATCAGCGCCCTTCCGGACTCCGTTATCTGCCACATCCTCTCTTTTCTCTCTACCAAGCAATCTGCCGCAACGAGCATCCTCTCCAAAAGATGGAAACCATTGTGGCTCGCAGTTTCTACTCTTGACTTCGACGAACGAAACTTTACAGACTTCAACACTTTTCGACTTTTCGTCTACTCAGTCATGCTCGCCCGTGACCCCACCCTGCCTATCCGATCGTTCCGCCTCAAAAGTCGGGGCATCTCTCCTGGTTTCAATCCTCATGATGTTAATAGATTTATTAATGCTGCTATGCAACGTGGAATTCAGAATCTCAACCTTGAGATAGGTTTCTTTAAGTTACCTACCTGTGTTTTTAGTTGCATTAACCTCACTGTTCTTAAGTTGATGTCGCTGAAAATACACGATCTTCCTGAGGTCAATTTTCCATTGCTCAAAACACTTCATTTGGATTCAATCTCGTTCCGCCCATCTGGTGGTGCTCATAGCCATAGGTGTTTTAAAAGGCTTCTCTCCGGGTGTCCTGTTTTAGAGGATTTGCATGCAACAAGTTTATTCCTGGGTCGTTTTCCGGATGAAGAGTTTAATGGCTTACACCAATTAGTGAGAGCAAATATTCATTCAATCCCAATGGCTTGGGTTCGTAATGCAAAGTTTCTATCCACAAAACTGGTATGAACCCCAATGGTTTATCATGTTAAAAACAAATTTGGTTGGTTTCTTTTATTTTTAATTTTTTGGTACAAAAGTTTGGTTGATTTCTAACGTTTGCTTTTGCAACACATGTAGAGGCGCTCATATGACTACCAGGTTCCCACCTTTCAAAATTTGACTCACATGGAGATTGGATTTGAATTGAGATATGATTGGCTTGGGAAGTGGAAGTGGTTGACAGAAGTGCTCCAAAATTGTCCTAACCTTCAGAATCTTACCATTCGCGAGGTCTTATTTGTTGATGTTAAATGCCGATAATTTTTTTTAATTATACTTCCATCATTTTGATTATTTTTTCTAAAAAAAATTGTTTGTTGTTTGATAGGATCCTAGTTATGAGAATGAATTTGACTATGACTATGACGATTGGATGGACCCACCAATTGTTCCAGAATGTCTTTCAACTCAGCTTAAAACATGCTCGCTGATAGCTTTTAAAGGCATGAAATGTGAGCTTCAGTTTGCAGAGTACATTTTGAAGAGTGCAAAAGCACTGCACACCATGAAAATTAGTGCCTCACCGGTGGATTTAAATATAAAGCTCCAGATGCTAATGAAATTATCTTTGTGTCCAAGGGGCTCTACTACATGTAAACTCTCATTTGAATGA
Protein sequence

>MS.gene30044.t1

MHLVEVVDLGHVKKKKAVHVFINAAMQRGIQNLNLEIGFFKLPTCVFSCINLTVLKLMSLKIHDLPEVNFPLLKTLHLDSISFRPSGGAHSHRCFKRLLSGCPVLEDLHATSLFLGRFPDEEFNGLHQLVRANIHSIPMAWVRNAKFLSTKLRRSYDYQVPTFQNLTHMEIGFELRYDWLGKWKWLTEVLQNCPNLQNLTIREDPSYENEFDYDYDDWMDPPIVPECLSTQLKTCSLIAFKGMKCELQFAEYILKSAKALHTMKISASPVDLNIKLQMLMKLSLCPRGSTTCKLSFE