AlfalfaGEDB | MS.gene026015

M. sativa cultivar XinJiangDaYe / MS.gene026015

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF07939.14 PF12796.10 PF13414.9 PF13606.9 PF13637.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene026015.t1	XP_028219228.1	67.4	657	147	10	27	626	11	657	3.00E-147	532.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene026015.t1	Q54DA8	40.5	84	47	1	532	612	36	119	8.0e-08	60.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene026015.t1	A0A072U5A8	93.7	460	10	2	186	626	1	460	9.0e-138	500.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene008596	MS.gene026015	PPI
MS.gene67914	MS.gene026015	PPI
MS.gene053586	MS.gene026015	PPI
MS.gene42267	MS.gene026015	PPI
MS.gene97791	MS.gene026015	PPI
MS.gene008597	MS.gene026015	PPI
MS.gene35899	MS.gene026015	PPI
MS.gene026015	MS.gene02439	PPI
MS.gene09990	MS.gene026015	PPI
MS.gene49551	MS.gene026015	PPI
MS.gene54959	MS.gene026015	PPI
MS.gene026015	MS.gene048585	PPI
MS.gene026015	MS.gene009784	PPI
MS.gene033500	MS.gene026015	PPI
MS.gene040253	MS.gene026015	PPI
MS.gene026015	MS.gene50223	PPI
MS.gene51922	MS.gene026015	PPI
MS.gene026015	MS.gene028491	PPI
MS.gene026015	MS.gene60518	PPI
MS.gene009786	MS.gene026015	PPI
MS.gene98038	MS.gene026015	PPI
MS.gene35902	MS.gene026015	PPI
MS.gene50222	MS.gene026015	PPI
MS.gene026015	MS.gene35901	PPI
MS.gene50224	MS.gene026015	PPI
MS.gene35900	MS.gene026015	PPI
MS.gene026015	MS.gene024337	PPI
MS.gene035057	MS.gene026015	PPI
MS.gene026015	MS.gene36943	PPI
MS.gene01653	MS.gene026015	PPI
MS.gene30044	MS.gene026015	PPI
MS.gene67917	MS.gene026015	PPI
MS.gene026015	MS.gene009785	PPI
MS.gene36764	MS.gene026015	PPI
MS.gene36942	MS.gene026015	PPI
MS.gene047137	MS.gene026015	PPI
MS.gene026015	MS.gene032821	PPI
MS.gene36763	MS.gene026015	PPI
MS.gene024340	MS.gene026015	PPI
MS.gene026015	MS.gene024262	PPI
MS.gene36804	MS.gene026015	PPI
MS.gene026015	MS.gene60522	PPI
MS.gene026015	MS.gene80861	PPI
MS.gene047136	MS.gene026015	PPI
MS.gene055754	MS.gene026015	PPI
MS.gene026015	MS.gene49202	PPI
MS.gene36752	MS.gene026015	PPI
MS.gene032379	MS.gene026015	PPI
MS.gene78164	MS.gene026015	PPI
MS.gene32300	MS.gene026015	PPI
MS.gene52283	MS.gene026015	PPI
MS.gene68084	MS.gene026015	PPI
MS.gene026015	MS.gene045772	PPI
MS.gene26257	MS.gene026015	PPI
MS.gene49203	MS.gene026015	PPI
MS.gene026015	MS.gene90228	PPI
MS.gene30057	MS.gene026015	PPI
MS.gene026015	MS.gene91917	PPI
MS.gene09993	MS.gene026015	PPI
MS.gene054896	MS.gene026015	PPI
MS.gene68081	MS.gene026015	PPI
MS.gene026015	MS.gene51795	PPI
MS.gene23706	MS.gene026015	PPI
MS.gene072003	MS.gene026015	PPI
MS.gene072000	MS.gene026015	PPI
MS.gene010534	MS.gene026015	PPI
MS.gene024059	MS.gene026015	PPI
MS.gene065373	MS.gene026015	PPI
MS.gene072244	MS.gene026015	PPI
MS.gene002112	MS.gene026015	PPI
MS.gene51793	MS.gene026015	PPI
MS.gene071257	MS.gene026015	PPI
MS.gene59388	MS.gene026015	PPI
MS.gene54960	MS.gene026015	PPI
MS.gene026015	MS.gene033695	PPI
MS.gene033694	MS.gene026015	PPI
MS.gene026015	MS.gene60520	PPI
MS.gene026015	MS.gene068017	PPI
MS.gene36809	MS.gene026015	PPI
MS.gene026015	MS.gene30261	PPI
MS.gene32305	MS.gene026015	PPI
MS.gene36807	MS.gene026015	PPI
MS.gene36810	MS.gene026015	PPI
MS.gene64677	MS.gene026015	PPI
MS.gene048584	MS.gene026015	PPI
MS.gene026015	MS.gene52600	PPI
MS.gene36743	MS.gene026015	PPI
MS.gene007794	MS.gene026015	PPI
MS.gene026015	MS.gene074038	PPI
MS.gene026015	MS.gene072231	PPI
MS.gene026015	MS.gene017239	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene026015.t1	MTR_7g115280	94.130	460	8	2	186	626	1	460	0.0	865
MS.gene026015.t1	MTR_7g115290	97.814	183	4	0	1	183	1	183	9.91e-132	384
MS.gene026015.t1	MTR_1g080750	67.105	152	41	3	37	183	26	173	1.23e-57	192
MS.gene026015.t1	MTR_5g017450	41.304	138	58	6	57	182	43	169	5.78e-21	90.9
MS.gene026015.t1	MTR_1g116590	40.566	106	33	2	79	184	39	114	8.17e-19	82.8
MS.gene026015.t1	MTR_5g012030	41.905	105	58	1	504	605	2	106	6.27e-17	84.7
MS.gene026015.t1	MTR_5g012030	34.211	114	72	1	504	614	392	505	1.10e-14	77.8
MS.gene026015.t1	MTR_4g116160	34.043	188	119	3	258	440	70	257	5.55e-15	78.6
MS.gene026015.t1	MTR_4g116160	34.043	188	119	3	258	440	70	257	2.07e-14	77.0
MS.gene026015.t1	MTR_4g021880	24.933	373	221	12	203	550	101	439	2.72e-13	73.2
MS.gene026015.t1	MTR_4g021880	26.518	313	179	9	203	494	101	383	5.45e-13	71.6
MS.gene026015.t1	MTR_4g085970	34.000	150	75	5	43	182	13	148	1.10e-12	67.0
MS.gene026015.t1	MTR_1g041435	34.579	107	67	1	508	611	15	121	1.18e-12	69.7
MS.gene026015.t1	MTR_2g006870	32.704	159	100	3	283	440	533	685	1.23e-12	71.6
MS.gene026015.t1	MTR_5g043640	31.737	167	108	4	261	421	56	222	2.14e-12	67.8
MS.gene026015.t1	MTR_5g043640	30.573	157	82	4	205	360	96	226	7.35e-11	63.2
MS.gene026015.t1	MTR_2g096760	25.552	317	185	8	203	498	15	301	2.24e-12	70.1
MS.gene026015.t1	MTR_3g107720	29.612	206	133	5	298	497	543	742	3.81e-12	70.1
MS.gene026015.t1	MTR_1g112830	32.402	179	113	4	258	429	169	346	5.12e-12	69.3
MS.gene026015.t1	MTR_5g077770	28.634	227	149	5	216	440	522	737	8.62e-12	68.9
MS.gene026015.t1	MTR_5g022050	28.289	152	107	2	283	432	251	402	7.52e-11	65.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene026015.t1	AT3G04710	59.414	648	190	7	40	621	32	672	0.0	712
MS.gene026015.t1	AT3G04710	65.055	455	133	4	186	621	1	448	0.0	575
MS.gene026015.t1	AT3G04710	65.055	455	132	5	186	621	1	447	0.0	570
MS.gene026015.t1	AT1G08790	54.248	153	58	3	40	183	37	186	1.18e-44	157
MS.gene026015.t1	AT3G04700	55.414	157	57	2	40	183	32	188	9.49e-44	155
MS.gene026015.t1	AT5G28690	54.777	157	58	3	40	183	33	189	2.86e-39	143
MS.gene026015.t1	AT5G28690	54.777	157	58	3	40	183	33	189	2.86e-39	143
MS.gene026015.t1	AT5G28690	54.777	157	58	3	40	183	33	189	2.86e-39	143
MS.gene026015.t1	AT2G31560	40.566	106	58	2	79	184	100	200	1.58e-20	90.5
MS.gene026015.t1	AT2G31560	40.566	106	58	2	79	184	100	200	1.58e-20	90.5
MS.gene026015.t1	AT2G31560	40.566	106	58	2	79	184	100	200	1.58e-20	90.5
MS.gene026015.t1	AT2G43340	43.396	106	55	2	79	184	87	187	1.46e-19	87.4
MS.gene026015.t1	AT1G05870	40.741	108	56	3	79	184	86	187	1.60e-19	87.4
MS.gene026015.t1	AT1G05870	40.741	108	56	3	79	184	86	187	1.60e-19	87.4
MS.gene026015.t1	AT1G05870	40.741	108	56	3	79	184	86	187	1.60e-19	87.4
MS.gene026015.t1	AT1G05870	40.741	108	56	3	79	184	86	187	1.60e-19	87.4
MS.gene026015.t1	AT1G05870	40.741	108	56	3	79	184	86	187	1.60e-19	87.4
MS.gene026015.t1	AT1G05870	40.741	108	56	3	79	184	86	187	1.60e-19	87.4
MS.gene026015.t1	AT1G05870	40.741	108	56	3	79	184	86	187	1.60e-19	87.4
MS.gene026015.t1	AT1G05870	40.741	108	56	3	79	184	86	187	1.60e-19	87.4
MS.gene026015.t1	AT1G05870	40.741	108	56	3	79	184	86	187	1.60e-19	87.4
MS.gene026015.t1	AT1G05870	40.741	108	56	3	79	184	86	187	1.60e-19	87.4
MS.gene026015.t1	AT1G05870	40.741	108	56	3	79	184	86	187	1.60e-19	87.4
MS.gene026015.t1	AT1G05870	40.741	108	56	3	79	184	86	187	1.60e-19	87.4
MS.gene026015.t1	AT4G12400	43.750	112	60	1	504	612	2	113	1.84e-19	92.8
MS.gene026015.t1	AT4G12400	35.088	114	71	1	504	614	369	482	6.28e-16	81.6
MS.gene026015.t1	AT4G12400	43.750	112	60	1	504	612	2	113	2.04e-19	92.4
MS.gene026015.t1	AT4G12400	35.088	114	71	1	504	614	369	482	7.00e-16	81.3
MS.gene026015.t1	AT4G33985	39.844	128	62	4	59	182	30	146	3.66e-17	79.3
MS.gene026015.t1	AT1G62740	39.286	112	65	1	504	612	2	113	1.26e-16	84.0
MS.gene026015.t1	AT1G62740	33.929	112	71	1	506	614	384	495	5.15e-15	79.0
MS.gene026015.t1	AT3G50350	37.692	130	61	4	59	182	50	165	1.72e-14	72.4
MS.gene026015.t1	AT1G12270	39.286	112	65	1	504	612	2	113	4.20e-14	75.9
MS.gene026015.t1	AT1G12270	34.821	112	70	1	506	614	385	496	4.96e-14	75.9
MS.gene026015.t1	AT1G04190	37.624	101	60	1	508	605	19	119	4.75e-14	74.3
MS.gene026015.t1	AT5G20350	33.696	184	113	4	258	434	65	246	2.83e-13	73.6
MS.gene026015.t1	AT5G37500	33.708	178	114	4	265	440	538	713	1.05e-12	72.0
MS.gene026015.t1	AT2G15590	37.383	107	52	2	79	182	48	142	1.53e-12	66.2
MS.gene026015.t1	AT4G32500	28.774	212	139	5	294	501	545	748	4.81e-12	69.7
MS.gene026015.t1	AT2G15610	35.659	129	62	6	43	159	11	130	2.01e-11	62.8

Find 133 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTACCATTGCTAGCAATTT+AGG	0.149520	7.1:+2138890	MS.gene026015:CDS
AGAAGGAATAACATCAATTT+TGG	0.161591	7.1:-2141328	None:intergenic
ATCTGTTGCAGCTGATTCTT+TGG	0.215917	7.1:+2140133	MS.gene026015:CDS
TTTAATTGCTTCCACTGTTT+TGG	0.221129	7.1:-2138678	None:intergenic
AAATTGATGTTATTCCTTCT+TGG	0.223369	7.1:+2141331	MS.gene026015:CDS
TTGAGCCCAGTGCCTTAATT+TGG	0.274017	7.1:-2137038	None:intergenic
CTCAGAGGATGTATTGAATT+AGG	0.280954	7.1:+2136360	MS.gene026015:CDS
ATTACAGATTGTTCTCGATA+TGG	0.285003	7.1:+2138444	MS.gene026015:intron
ATTGGTCCAAAGCTTGTTAT+AGG	0.291093	7.1:+2143192	MS.gene026015:CDS
TAGCGAGGAAATAGAGTTAC+AGG	0.292376	7.1:+2136113	MS.gene026015:CDS
CTTCCAGCTGCAACCTGTAT+TGG	0.297839	7.1:-2141264	None:intergenic
TGACAACTACGGTTTATCTT+TGG	0.308207	7.1:-2143756	None:intergenic
GGATGTATTGAATTAGGATT+TGG	0.322686	7.1:+2136366	MS.gene026015:CDS
TTCGGGAACACCGTTGGTTT+GGG	0.325527	7.1:+2139329	MS.gene026015:CDS
ATTCGGGAACACCGTTGGTT+TGG	0.327829	7.1:+2139328	MS.gene026015:CDS
ACACCGTTGGTTTGGGCTGC+TGG	0.331465	7.1:+2139336	MS.gene026015:CDS
TTGCACATTGCAGCTGATAA+TGG	0.333370	7.1:+2140899	MS.gene026015:CDS
TTGCATCATTCTGCTGGAAT+AGG	0.335655	7.1:+2138923	MS.gene026015:CDS
ATTCCTTCTTGGACTATTGA+TGG	0.346294	7.1:+2141342	MS.gene026015:CDS
TCTCATTCATGCTGCACGTC+AGG	0.349718	7.1:+2138823	MS.gene026015:CDS
TGAGCAGTGCGATGCGTTGT+AGG	0.359078	7.1:-2136489	None:intergenic
GATCAGTTCCTGCTTTCAAC+AGG	0.361386	7.1:-2140943	None:intergenic
TCAAGACTTTACTTGTGTAT+AGG	0.364581	7.1:-2142816	None:intergenic
CTCTCCCATGCCATGTCTCC+TGG	0.364978	7.1:-2136270	None:intergenic
GCATTGTTGATTCTGATTCT+TGG	0.366062	7.1:+2136550	MS.gene026015:CDS
CCCGAATCACTTTCTAAGTC+AGG	0.367297	7.1:-2139312	None:intergenic
TTTACAAGCTCCTTGCTCTC+AGG	0.376147	7.1:-2143380	None:intergenic
TCCTGACTTAGAAAGTGATT+CGG	0.397766	7.1:+2139311	MS.gene026015:CDS
AAAGCTTTGCAATACGTTAC+CGG	0.401532	7.1:+2136410	MS.gene026015:CDS
TTTGGATTTAATGAGGAAGA+TGG	0.407281	7.1:+2136384	MS.gene026015:CDS
CCATCATCAGTTTCAGCATT+GGG	0.414646	7.1:-2140092	None:intergenic
CCTGACTTAGAAAGTGATTC+GGG	0.427650	7.1:+2139312	MS.gene026015:CDS
CCGGACTAGGAGACAGGTTC+CGG	0.434499	7.1:-2136458	None:intergenic
CTTTAAGAGATCGAGATTGC+CGG	0.435481	7.1:-2138533	None:intergenic
AGGAGCTTGTAAATGCGTTC+AGG	0.442269	7.1:+2143390	MS.gene026015:CDS
AGGCATTGGCGTTTAAGAAA+AGG	0.442446	7.1:+2136211	MS.gene026015:CDS
GCCTTCCCTATAACAAGCTT+TGG	0.445915	7.1:-2143198	None:intergenic
TGCAAGTGCGTCGGAAGCAT+CGG	0.447294	7.1:-2138470	None:intergenic
GAAGCTGGAAGAAAATTTCA+TGG	0.454075	7.1:+2143728	MS.gene026015:CDS
TCGTCTATTGCGAGTCCTAC+TGG	0.454107	7.1:+2136525	MS.gene026015:CDS
ACGGCTTTGCATCATTCTGC+TGG	0.454550	7.1:+2138917	MS.gene026015:CDS
ATCAAAATCAAGAGGGGATG+AGG	0.458977	7.1:+2142757	MS.gene026015:CDS
ATGCTTAGAGCTGTTAATAC+AGG	0.462858	7.1:+2140157	MS.gene026015:CDS
TTGGTCCAAAGCTTGTTATA+GGG	0.462903	7.1:+2143193	MS.gene026015:CDS
TAAATGCTAAAGAAAATAAC+CGG	0.465720	7.1:+2142101	MS.gene026015:CDS
TAACAAGCTTTGGACCAATC+TGG	0.465796	7.1:-2143188	None:intergenic
TTCCTTCTTGGACTATTGAT+GGG	0.466672	7.1:+2141343	MS.gene026015:CDS
GAAGCTAGGTCAAGCTGAGC+AGG	0.467307	7.1:+2143130	MS.gene026015:CDS
TACAGGGAAGCTGTTGAAGC+TGG	0.468882	7.1:+2143713	MS.gene026015:intron
TAAGTCAGGATTAACTCCTC+TGG	0.476327	7.1:-2139298	None:intergenic
CTTCCGACGCACTTGCAGTT+AGG	0.476513	7.1:+2138476	MS.gene026015:CDS
GCTAAGTATCTTATTGATCA+TGG	0.477834	7.1:+2138860	MS.gene026015:CDS
CCGGAACCTGTCTCCTAGTC+CGG	0.479308	7.1:+2136458	MS.gene026015:CDS
TCAGGAGGAAGAGTTGCAAC+AGG	0.481110	7.1:+2136167	MS.gene026015:CDS
GCAAGTGCGTCGGAAGCATC+GGG	0.488101	7.1:-2138469	None:intergenic
GCCATCATCAGTTTCAGCAT+TGG	0.492771	7.1:-2140093	None:intergenic
TGATCAGAAGGCAGAGGCAT+TGG	0.495884	7.1:+2136197	MS.gene026015:CDS
CTCACCTCGTCAGAGACATT+AGG	0.497830	7.1:-2140965	None:intergenic
TGAATGCAATGTACCATCGC+TGG	0.498194	7.1:-2143087	None:intergenic
ATAAATTCCCTGTTGAAAGC+AGG	0.500352	7.1:+2140935	MS.gene026015:CDS
TTACAAGCTCCTTGCTCTCA+GGG	0.500721	7.1:-2143379	None:intergenic
TAAAAGGAATGACTATCATA+CGG	0.521638	7.1:+2142784	MS.gene026015:CDS
ATTAGGATTTGGATTTAATG+AGG	0.528015	7.1:+2136377	MS.gene026015:CDS
CTATCAATGTGTGGAACACC+AGG	0.530999	7.1:+2136252	MS.gene026015:CDS
AATACAGGTTGCAGCTGGAA+GGG	0.537141	7.1:+2141266	MS.gene026015:CDS
GGATGGTGTTAAGCCAATAC+AGG	0.538177	7.1:+2141251	MS.gene026015:intron
CAATACAGGTTGCAGCTGGA+AGG	0.538296	7.1:+2141265	MS.gene026015:CDS
GAATGCAATGTACCATCGCT+GGG	0.539778	7.1:-2143086	None:intergenic
AAGCCAATACAGGTTGCAGC+TGG	0.541821	7.1:+2141261	MS.gene026015:CDS
GAAATAGAGTTACAGGGAGG+AGG	0.543729	7.1:+2136120	MS.gene026015:CDS
CTCATTCATGCTGCACGTCA+GGG	0.549715	7.1:+2138824	MS.gene026015:CDS
AACACTTGACCCTGAGAGCA+AGG	0.550632	7.1:+2143370	MS.gene026015:CDS
ATACAGGTTGCAGCTGGAAG+GGG	0.552229	7.1:+2141267	MS.gene026015:CDS
GAGACATGGCATGGGAGAGA+AGG	0.556511	7.1:+2136274	MS.gene026015:CDS
TGCTAGCAATTTAGGAACCA+CGG	0.556932	7.1:+2138898	MS.gene026015:CDS
AGTCTATGTTGGATGAAGCT+AGG	0.562469	7.1:+2143116	MS.gene026015:CDS
TAGATACCGGACTAGGAGAC+AGG	0.562946	7.1:-2136464	None:intergenic
TTCCTCAACGCTGCTCGTAC+CGG	0.562981	7.1:+2138514	MS.gene026015:CDS
ATTCAAATAGAAGTCTATGT+TGG	0.563771	7.1:+2143105	MS.gene026015:CDS
TCTTGGAAGATTTGTAACCC+AGG	0.564321	7.1:+2136567	MS.gene026015:CDS
AAAGTAAATGTTAGTGCTGG+TGG	0.569289	7.1:+2140866	MS.gene026015:CDS
GAACACCAGGAGACATGGCA+TGG	0.570374	7.1:+2136265	MS.gene026015:CDS
ACAGGAACATTCTGATCAGA+AGG	0.570710	7.1:+2136185	MS.gene026015:CDS
CTTGAGCATTTGCTGTCCAG+AGG	0.572302	7.1:+2139282	MS.gene026015:CDS
TCCAAAGCTTGTTATAGGGA+AGG	0.574646	7.1:+2143197	MS.gene026015:CDS
CTGCAACAGATGAAATGAGT+GGG	0.576458	7.1:-2140121	None:intergenic
GTGTGGAACACCAGGAGACA+TGG	0.578541	7.1:+2136260	MS.gene026015:CDS
GTCGACAAACTCAACGTAGG+AGG	0.579261	7.1:+2136298	MS.gene026015:CDS
GCAAAATATAGATCAAGAGC+CGG	0.579769	7.1:-2136429	None:intergenic
CCTAAATTGCTAGCAATGGT+AGG	0.580362	7.1:-2138890	None:intergenic
ATCCCATCAATAGTCCAAGA+AGG	0.585457	7.1:-2141345	None:intergenic
CAGCAGAATCAAAATCAAGA+GGG	0.593887	7.1:+2142750	MS.gene026015:CDS
ATCAGTTCCTGCTTTCAACA+GGG	0.598606	7.1:-2140942	None:intergenic
GTAGGTGTAGATACCGGACT+AGG	0.601321	7.1:-2136471	None:intergenic
CCCAATGCTGAAACTGATGA+TGG	0.604551	7.1:+2140092	MS.gene026015:CDS
TTATCAGCTGCAATGTGCAA+AGG	0.606996	7.1:-2140896	None:intergenic
GTAGAGCATTAAGACCAGAT+TGG	0.608756	7.1:+2143174	MS.gene026015:CDS
GGTTCCTAAATTGCTAGCAA+TGG	0.609817	7.1:-2138894	None:intergenic
CTGCCTAACTGCAAGTGCGT+CGG	0.612225	7.1:-2138479	None:intergenic
AAGTGATTCGGGAACACCGT+TGG	0.612822	7.1:+2139323	MS.gene026015:CDS
TGCCGGTACGAGCAGCGTTG+AGG	0.612947	7.1:-2138516	None:intergenic
AAACAGCAGCATCCACAGAC+CGG	0.613311	7.1:-2142120	None:intergenic
TGCGTTGTAGGTGTAGATAC+CGG	0.613366	7.1:-2136477	None:intergenic
ATCAATAAGATACTTAGCAG+TGG	0.617709	7.1:-2138855	None:intergenic
ACATTCTGATCAGAAGGCAG+AGG	0.617952	7.1:+2136191	MS.gene026015:CDS
GCAGCAGAATCAAAATCAAG+AGG	0.620760	7.1:+2142749	MS.gene026015:CDS
GCTAAAGTAAATGTTAGTGC+TGG	0.624109	7.1:+2140863	MS.gene026015:CDS
TGACCAGCAGCCCAAACCAA+CGG	0.624815	7.1:-2139339	None:intergenic
ACATGCCAGCAACAAACGCA+AGG	0.626173	7.1:-2143225	None:intergenic
CAAAGATTTATCCAAAACAG+TGG	0.629444	7.1:+2138667	MS.gene026015:CDS
GCAGGCTTTAGCTGATGCAA+AGG	0.635071	7.1:+2143148	MS.gene026015:CDS
GAATCAACAATGCTACCAGT+AGG	0.635740	7.1:-2136540	None:intergenic
ATTGATTTGAATCCCAGCGA+TGG	0.637054	7.1:+2143074	MS.gene026015:CDS
ATTAAAGATGCAAACAAGCG+TGG	0.639246	7.1:+2138695	MS.gene026015:CDS
GCAGCTGGAAGGGGAAATCG+TGG	0.646088	7.1:+2141276	MS.gene026015:CDS
TAAAGAAAATAACCGGTCTG+TGG	0.653075	7.1:+2142108	MS.gene026015:CDS
AGCAGAATGATGCAAAGCCG+TGG	0.654699	7.1:-2138915	None:intergenic
TCGCAATAGACGAAGATGAA+CGG	0.657250	7.1:-2136515	None:intergenic
GCTGCAACAGATGAAATGAG+TGG	0.659045	7.1:-2140122	None:intergenic
ACAAAGCAACTATCAATGTG+TGG	0.659661	7.1:+2136243	MS.gene026015:CDS
AATTAATATTATGAGTAGCG+AGG	0.661289	7.1:+2136098	None:intergenic
AGCAGAATCAAAATCAAGAG+GGG	0.665063	7.1:+2142751	MS.gene026015:CDS
AACACCAGGAGACATGGCAT+GGG	0.674784	7.1:+2136266	MS.gene026015:CDS
ATTTGCGGAACAATTAGACG+AGG	0.679877	7.1:+2138643	MS.gene026015:CDS
TTTGCGGAACAATTAGACGA+GGG	0.690385	7.1:+2138644	MS.gene026015:CDS
GTCACTGTTCTATTAAAACA+TGG	0.691599	7.1:+2139378	MS.gene026015:CDS
TGCAACAGATGAAATGAGTG+GGG	0.693780	7.1:-2140120	None:intergenic
AGCGAGGAAATAGAGTTACA+GGG	0.695690	7.1:+2136114	MS.gene026015:CDS
TGATCCTAATGTCTCTGACG+AGG	0.709103	7.1:+2140961	MS.gene026015:CDS
GAAGATCTAAATGAGCTCAG+AGG	0.722824	7.1:+2136345	MS.gene026015:CDS
GGCGTCGACAAACTCAACGT+AGG	0.748571	7.1:+2136295	MS.gene026015:CDS
TCGAGAGCAAAATATCCCTG+AGG	0.758992	7.1:+2142144	MS.gene026015:CDS
GAGGAAATAGAGTTACAGGG+AGG	0.768483	7.1:+2136117	MS.gene026015:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	2136108	2143772	2136108	ID=MS.gene026015
chr7.1	mRNA	2136108	2143772	2136108	ID=MS.gene026015.t1;Parent=MS.gene026015
chr7.1	exon	2136108	2136588	2136108	ID=MS.gene026015.t1.exon1;Parent=MS.gene026015.t1
chr7.1	CDS	2136108	2136588	2136108	ID=cds.MS.gene026015.t1;Parent=MS.gene026015.t1
chr7.1	exon	2137014	2137075	2137014	ID=MS.gene026015.t1.exon2;Parent=MS.gene026015.t1
chr7.1	CDS	2137014	2137075	2137014	ID=cds.MS.gene026015.t1;Parent=MS.gene026015.t1
chr7.1	exon	2138451	2138556	2138451	ID=MS.gene026015.t1.exon3;Parent=MS.gene026015.t1
chr7.1	CDS	2138451	2138556	2138451	ID=cds.MS.gene026015.t1;Parent=MS.gene026015.t1
chr7.1	exon	2138642	2138761	2138642	ID=MS.gene026015.t1.exon4;Parent=MS.gene026015.t1
chr7.1	CDS	2138642	2138761	2138642	ID=cds.MS.gene026015.t1;Parent=MS.gene026015.t1
chr7.1	exon	2138810	2138944	2138810	ID=MS.gene026015.t1.exon5;Parent=MS.gene026015.t1
chr7.1	CDS	2138810	2138944	2138810	ID=cds.MS.gene026015.t1;Parent=MS.gene026015.t1
chr7.1	exon	2139268	2139407	2139268	ID=MS.gene026015.t1.exon6;Parent=MS.gene026015.t1
chr7.1	CDS	2139268	2139407	2139268	ID=cds.MS.gene026015.t1;Parent=MS.gene026015.t1
chr7.1	exon	2140092	2140178	2140092	ID=MS.gene026015.t1.exon7;Parent=MS.gene026015.t1
chr7.1	CDS	2140092	2140178	2140092	ID=cds.MS.gene026015.t1;Parent=MS.gene026015.t1
chr7.1	exon	2140857	2140982	2140857	ID=MS.gene026015.t1.exon8;Parent=MS.gene026015.t1
chr7.1	CDS	2140857	2140982	2140857	ID=cds.MS.gene026015.t1;Parent=MS.gene026015.t1
chr7.1	exon	2141252	2141404	2141252	ID=MS.gene026015.t1.exon9;Parent=MS.gene026015.t1
chr7.1	CDS	2141252	2141404	2141252	ID=cds.MS.gene026015.t1;Parent=MS.gene026015.t1
chr7.1	exon	2142091	2142165	2142091	ID=MS.gene026015.t1.exon10;Parent=MS.gene026015.t1
chr7.1	CDS	2142091	2142165	2142091	ID=cds.MS.gene026015.t1;Parent=MS.gene026015.t1
chr7.1	exon	2142725	2142826	2142725	ID=MS.gene026015.t1.exon11;Parent=MS.gene026015.t1
chr7.1	CDS	2142725	2142826	2142725	ID=cds.MS.gene026015.t1;Parent=MS.gene026015.t1
chr7.1	exon	2143071	2143234	2143071	ID=MS.gene026015.t1.exon12;Parent=MS.gene026015.t1
chr7.1	CDS	2143071	2143234	2143071	ID=cds.MS.gene026015.t1;Parent=MS.gene026015.t1
chr7.1	exon	2143334	2143411	2143334	ID=MS.gene026015.t1.exon13;Parent=MS.gene026015.t1
chr7.1	CDS	2143334	2143411	2143334	ID=cds.MS.gene026015.t1;Parent=MS.gene026015.t1
chr7.1	exon	2143718	2143772	2143718	ID=MS.gene026015.t1.exon14;Parent=MS.gene026015.t1
chr7.1	CDS	2143718	2143772	2143718	ID=cds.MS.gene026015.t1;Parent=MS.gene026015.t1

Gene Sequence

>MS.gene026015

ATGAGTAGCGAGGAAATAGAGTTACAGGGAGGAGGAGTCTGCGAAAACGAGTTTTCAGATCAGGAGGAAGAGTTGCAACAGGAACATTCTGATCAGAAGGCAGAGGCATTGGCGTTTAAGAAAAGGAAACGTCTAACAAAGCAACTATCAATGTGTGGAACACCAGGAGACATGGCATGGGAGAGAAGGCGTCGACAAACTCAACGTAGGAGGAATAGCATGCACGATTGCAACGACGAAGATCTAAATGAGCTCAGAGGATGTATTGAATTAGGATTTGGATTTAATGAGGAAGATGGACAAAAGCTTTGCAATACGTTACCGGCTCTTGATCTATATTTTGCTGTTAACCGGAACCTGTCTCCTAGTCCGGTATCTACACCTACAACGCATCGCACTGCTCACAGCCGTTCATCTTCGTCTATTGCGAGTCCTACTGGTAGCATTGTTGATTCTGATTCTTGGAAGATTTGTAACCCAGGTAATTAATGTCACTTATTCATTAATTACCCAATTATTCTAAATTGATGTCGTGGTTTCAAGTATAGTTACGTTGCAAACTTTAACATTGTAACAGATAGCAATGCAAACAAATGCAGCCAATGCGCTGCAATTGTGATTGTTATCATTGTTTTTGAGACTTCTAAAATATTTATACTAGAGTTGTAGACCAACTTAAAACTAATATGATCTCAAATATAATACATCACTAATTATAATAAATTTGTAAGTATCGTTTGTTACAAAATATTGATAGTGTCCAAGTACTACTATAAATGTTTCAAAATAATATTCGGCTGTGTTCATTGTATATTATAAACGTAGATATGTTCGACTCTGGCTTTTAGTCTGTAATTAATTTGATGAATTGATTGATTAATGTCGGGTATTGTAATCTGGCTGCAGGGGATGATCCAGCACTTATAAAAACCAAATTAAGGCACTGGGCTCAAGCTGTTGCTTGTTCAGTAATGCAATCTCATTCATGATGAAGTCAAGTGTGGGATACATACATCATACATATGTTCTGCTTTTCTTTCTTTTTTTGTACAGAGGGAGGGTGGTTCTCATTCTAGATATATGAAAAATAAATAAAAAAATAAAAGAAAAAAACTACCAAAAATTTAAAAAGAAAAAAAAAATGTGCAGATTTTCTTGAGGGTTTTTGTTGGGGCTGAACAAACAAACACATTGGTTGGTTGGCTTGGCTCCAATTTGTAAATTGGTAGAAGGTTATGGTGTCATCAAATATTTGCACCATTTCCCTTTACCTTTTTTTCCCTCTTTATTTGTATATTTTTTCAATTGGATGTGGAACTGTCTCTCCGAGGAGCTATTCATTTCATGAAAACAATTTTGAATTGATCCACAACTGGTAAATGCTTTTTATAATATGAGCACTAAGCAAGGGCTTTTAGTTAGGCTCCAATTGTAAGGTTCCATAACTTTATAAAGATAATTGTTGATTTTGATTTCAAATGTAAGTAGTAAACATGTATATGTATATATGTATTTGTGTAAATTTAAATCAAATATAGTATTTTTTTCCTTACATTTAAGATTAGAATATAGTTGTCAATTGCTCTCAAAACTTGGTCTCAACTCACATGCCGACTCTCAGTACTAATATGGTTGATTTTGGCTCCCATATATACGCAAATATAGGCTACGAATTATAGAATCTAAAACATGTAACTTTGTTTAAAAAAAGTTAATTGACTTAGAAGTGGTTTAAATGATTTTAAATTATGGGTTCTGATTTTACATGTAACTTTGTTTAAAAAAAGTTAATTGACTTAGAAGTGGTTCAAATGATTTATATTATGTGTTCTGATTTTACAATTCAATACTCCAAAAACACGATTTGGATTATAGAATCCAAACGGAGGTAATTGTGGGAGGCTGGGCGGGGGGAAGGGTGATTTTACTCTTTTTTTTTTTTTTTTCCGTTAATTGCTCAAATTGGCCTCTTGTCCACCAAAAATTGAATTATTCAATATCATAAAAGTTTGTCTTATGTTATTTTATCTTGTTCTCTTCTCTATCCGATACTTAGTGTTTAACATATCAAACGCACGCTAAGTTATTTTTATAGACTTCGCGTATATTACAGACATGGTGAAAAATATGAACAATCTCAAATTACATGGTTAAAACCATTAATCCTTTTTTATTTTGTGAAAACAAACTCTTATCTTAATTGTGAGCTGCAGTTACGCATATTAGGGTTTGAGCCCACGGCCCAAAACATAACCTTTATATATGTTGACTACTCTTGAGTAAGGTTTTGGTTTCTTTGAATTGTTTCAACTTTCAACACAGGGTTTTGGTTTTGCATTACAGATTGTTCTCGATATGGCTCCCGATGCTTCCGACGCACTTGCAGTTAGGCAGAAAGTTCAACACTTCCTCAACGCTGCTCGTACCGGCAATCTCGATCTCTTAAAGAGTAACTCAATCATTCAATTAATCAACTTATTATTATGTAATACTTCGTTGTTCTTCATTCATCTGATTTTATATTGTTTTCATAGAATTTGCGGAACAATTAGACGAGGGCAAAGATTTATCCAAAACAGTGGAAGCAATTAAAGATGCAAACAAGCGTGGTGCACTTCATTTTGCTGCTCTTGAAGGACAAACTCAGATTTGCAGTTATTTTGTTGAGGATTTGAAGCTTCGTATTGATGATAAAGACGAAGACGGTGAAACTGCTCTCATTCATGCTGCACGTCAGGGACACACTGCCACTGCTAAGTATCTTATTGATCATGGTGCTGATCCTACCATTGCTAGCAATTTAGGAACCACGGCTTTGCATCATTCTGCTGGAATAGGTTTTTTCCTTTCTTTATTATTCTGTTACGTGATGAATTGATTTTGATTGCTCTATGTCCATGTTTGGAGAAACATCTTAATTAAGTGCTTATAGTATAGTATGACAAAAGATAGAATAAGTCAAACTGTTTTCATAAGTTATCCTTGAGAGCTTATGGACTGATAACAAGTGTTTATGTCATTAGAAAATCTCTAATTAGCGAATCCAAACAGAAACTATCTCTTTTTATGCAATGTTATTAGAAAAAGTATTGTGCTGTTAAGTTTGTCTCTCTTGTTATGTGCCTGCTGATTTTGTTGTGATATGTGGTTTTTGTTTCAGGAGACACGGATTTACTTGAGCATTTGCTGTCCAGAGGAGTTAATCCTGACTTAGAAAGTGATTCGGGAACACCGTTGGTTTGGGCTGCTGGTCATGCTCAAGAAGCTGCTGTCACTGTTCTATTAAAACATGGTGCAAATGTAATTCTTCATTCCATTTCTTAATTTGTGTTACTTTGTGATAATGTTACGAAGTATACTCGATTTCTTTTTTCAGAAATTTGATTCGTTCGAGTATCAGAATTCTATATCTATTTGATTGGATATTTAGAGTATCAATTGGTGTTTACACTACGCATATTCTTTTCTGTTTCTTCTTTCTAAAATTGCTTTGTTCTTCCTATCATCTGGATCTGTGGTCTTCTGAAGAAACAGTCATTTACCACATTTCGATAGTCAATATGCTCTAGTTTCATTTCAGCAATGTTATCTGACTTTTACATCAATATTTTATTGTTCAAATCGATAAGCGCAATCTTCATAAGCTTTACTGATACACTCTGTCATTGTTGAGATACATTTTCTTGAATAGTTTACGAGGATTGATTAATTTATTCTTCTTGCTTGATTCATCACATCCTAATGAGACAATGTATTATAGCTTGTTTATTTGCCACTCTAGAAAACGATTCTGCATTCTAGTGTACTTTTGTTTTTGTGTACTACTAATATGCTTATTACATGGGCATGGTTGAACAAATTTTCTTTCTTATCTATGCCGTGTTAAATTTTCAATTTAGTTTCTGAGATATTTTACAGAATATCACTATTGTAATAGTATTACAAATATAATGCTTTCCCTTTCATCTTGGTGTTGTTTTGCAGCCCAATGCTGAAACTGATGATGGCATTACCCCACTCATTTCATCTGTTGCAGCTGATTCTTTGGCATGCTTAGAGCTGTTAATACAGGTTCAACATCTTGTTCAACTTGCAACCCTTACTAAACAATATACTATGTTTATGATTTGGATATGTGAAACATGGAATAAATACTAGAATCTTTGCTGTAATATTATTATACTGATACAGAGATACTCCATGAAAATTGTAGAAAATTTATCAGTGGAAATGGTGGGAAAAACGATGTATATGATGAATTAGTATATATAACCAACATCATTCATAGAATCGTGATGATAAACTATACATTTAATGATTTCATTTGAGTGACATATTGTAGGAGCAATAATGCCATTAAAAATTAAACTGCTAGTATGATAATGTAATCAGGCTGACAATTTCATTCCATGTTATATATCCATAACAATTAGTTAGTTGATTAGTTCAAAGTATATTATATATGCCCAGTATTACAAAAATGTACTGGGAATCAGTGCTAGTGGGTGTTATTTACTGAACATCTATCTAATGAAAAAGGGAAGAGATAGATACATTGGTAAAATACGTTCAAAGCTATAGAAAGCTGGGTTGAACCTTGTATTTTGTTGATGAATGTACCTTAAAAGGCGTCTCAACAAGTACCCATCCCGTAGCCTTAAAAGTTTGTTATGCTATCCTAGTGAATATAGATTTCCTGTATATTTTTCAACTTCTGATCCATCTATATTTGGATTTTACTGTATACAGGCAGGTGCTAAAGTAAATGTTAGTGCTGGTGGAGCAACTCCTTTGCACATTGCAGCTGATAATGGAAGTCTAGAACTTATAAATTCCCTGTTGAAAGCAGGAACTGATCCTAATGTCTCTGACGAGGTGAGACGTATTTAATTTCCTAACTTCCTCATTTGGCTCATAACCTTCTGATGTTAGTATAGAACTCAATGGCTGCCAAACAATGCCGAGAGTCTACTTATATAAGAACCCAATAGTATTTTCTTGTATTCTTGTTTCCAAGATTTTTCCAATCTTTTCAGCGTCTCTGATGTCAACTTTGCTTATCCAGTGTAGTGCATATGGCTGTGGTAAACAATGTGCCTTCCATTAATATTTGGAACTAAATTTTATGTCTGTTTGGATCACAGGATGGTGTTAAGCCAATACAGGTTGCAGCTGGAAGGGGAAATCGTGGAGCTGTTGAGATATTGTTCCCCGTGACATCCAAAATTGATGTTATTCCTTCTTGGACTATTGATGGGATACTTGAGCATGTGCAATCAGAAAGTAAAAAGCAGCAGGTACTGAAGCCCTAATTTTTTCTATGTCCTGCTGGACAGAGTTAAGGAATATCATTGCTGCAAGAACATGAGATAATTACGCTTACTTTTGGTTAAGATAGATGAAGCCGTGCTTACAATAGTCACCGCATTATCTTAAAATACAAAGTATATTTGCCTTGTAGTTTCATAGGCTGTCAAGAATTAAATATTTTCTACATTTATTTTGTTACAAAGAAAAGGGAACTCTACAATTTTACTGTAGCTATCCAAATTAAAATGGAGGAACATAAAATATTCTCTGCTCAAGCCAGCATTCTCCAGCAAGGAATAAATCTATATGCGTAATGCTAAAATTCTTTAAACTTTAACTGATTGTGGTTGCAGTTTTTACTATTATATTGTCTTGATCATATTATACTCTTAAAGTTCAAAAAGGGTTAACTAATTCTTTCTGATACTAGTTTGTATTTTTGTCTCAAGCCAGCATTCTCCAGCAAGGAATAAATCTATATGCGTAATGCTAAAATTCTTTAAACTTTTAAGTGATTGTGGTTGCAGTTTTTACTATTATATTGTCTTGATCATATTATACTCTTAAAGTTCAAAAAGGGTTAACTAATTCTTTCTGATACTAGTTTGTATTTTTGTTGCCTGACAAAGTGGATTTCTTAATCTTTGTACTGAGTTGTAAGGGCAATGTGCAGGATGAATCGCTAAATGCTAAAGAAAATAACCGGTCTGTGGATGCTGCTGTTTCTCGAGAGCAAAATATCCCTGAGGTGTGTTTTCCTATTAAATATATTAGCTGTACTTTTTTCTCAATTTGGTATGTTACAGACTTACAGCTGTACTGTCAATGTAAGTAGCTGCAAAAATATTGTAGATGCTGAAGAAATATGCAAGTCGCATCCAATTATGTGTATTCAGTCATAAGAATCGTCTGTTTACGTAAATCTCTCTCGTTTCTAGATTGTGTATTGCTTCAGAGATTATGCACCATCTTTGTTGTTATCCGTTGCGTTCCCCTCAGATTTTGGCTGGTTATTTTAAAGATATTTAGTGAGCCTTTCTTCTTCTTAGGATTCTGTTTCTCATTTATCACTCTTCTGGTTTATTGTACCCTATCGAGTATGTGCACTGTCTTATTGAAAAAAAAAAAGTATGTGCACTGTCTTAAACTTTCTTACAAGATTATGATTTTAATACATTGTCAAGTTAGAACCCATGATCAATTGCTTATTCTTATAATAAAATTCGATTTGATTTTTGATCTTTTAGTTAAGTGTGTCAACTTTACTTTTTTTTTTCTTTGTAATATAATTGTGTGAACTCATCTAGGTGTCACCTGAAGCCAAAAAGAGAGCAGCAGAATCAAAATCAAGAGGGGATGAGGCTTTTAAAAGGAATGACTATCATACGGCTATAGATTCCTATACACAAGTAAAGTCTTGATCTCTCGTCTAACTCTTGAACTTCATTCTGTTTGTAACTTTTGCTTGTTGAGTACTTTTCGTGCACCATGCCCCGTGTTAGTTATGTCTCAATGATGTTTCTGTGCACTGTTCACTGGACATCCACATGTTTATCTAGTCTAGTTTTAGAAAATTAACTGCATTTATCTTCTTACTAAATGACAATCAGTTGATGATATATTCTTTTCTTTTCTTTTTGAATGTGTTTGCAGGCAATTGATTTGAATCCCAGCGATGGTACATTGCATTCAAATAGAAGTCTATGTTGGATGAAGCTAGGTCAAGCTGAGCAGGCTTTAGCTGATGCAAAGGCATGTAGAGCATTAAGACCAGATTGGTCCAAAGCTTGTTATAGGGAAGGCGCAGCCTTGCGTTTGTTGCTGGCATGTTATCCTGAAACCTTTCCCCCTTGACATTTGCTCCAGGACACATGATTCACGTTTCTTAACTTCAATTTGCTATCCTTGTTGCAGAGGTTTGATGAAGCTGCAAATGCTTTTTACGAGGGAGTAACACTTGACCCTGAGAGCAAGGAGCTTGTAAATGCGTTCAGGTCTACACATTGTTTTATTCAGACTTATTTGCACTTTTAGTTATTTTGCAATAGTTAATGTATGGTTTAGGTCTTCTAATAATTTATTGAGCAAATTAAATTTAATTCAATTAAGTCTCTTCATCATTTTTTCTACTTTAAACTCTCAAATTCATATTTTTGAATCAAAATCATCACAAATTTAACTGATCAAATCTTCAAATCGCTCAGATTCACCAATGCCAACCAGCACATACAAGTCTTTAATCTGTTGATCAGTTCATTAATTTGTATGTATCTAATCATGTATTCATTCATCTTTTACAGGGAAGCTGTTGAAGCTGGAAGAAAATTTCATGGCACAACCAAAGATAAACCGTAG

Protein sequence

>MS.gene026015.t1

MSSEEIELQGGGVCENEFSDQEEELQQEHSDQKAEALAFKKRKRLTKQLSMCGTPGDMAWERRRRQTQRRRNSMHDCNDEDLNELRGCIELGFGFNEEDGQKLCNTLPALDLYFAVNRNLSPSPVSTPTTHRTAHSRSSSSIASPTGSIVDSDSWKICNPGDDPALIKTKLRHWAQAVACSIVLDMAPDASDALAVRQKVQHFLNAARTGNLDLLKKFAEQLDEGKDLSKTVEAIKDANKRGALHFAALEGQTQICNGETALIHAARQGHTATAKYLIDHGADPTIASNLGTTALHHSAGIGDTDLLEHLLSRGVNPDLESDSGTPLVWAAGHAQEAAVTVLLKHGANPNAETDDGITPLISSVAADSLACLELLIQAGAKVNVSAGGATPLHIAADNGSLELINSLLKAGTDPNVSDEDGVKPIQVAAGRGNRGAVEILFPVTSKIDVIPSWTIDGILEHVQSESKKQQDESLNAKENNRSVDAAVSREQNIPEVSPEAKKRAAESKSRGDEAFKRNDYHTAIDSYTQAIDLNPSDGTLHSNRSLCWMKLGQAEQALADAKACRALRPDWSKACYREGAALRLFDEAANAFYEGVTLDPESKELVNAFREAVEAGRKFHGTTKDKP