AlfalfaGEDB | MS.gene97791

M. sativa cultivar XinJiangDaYe / MS.gene97791

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97791.t1	RHN81316.1	89.9	189	19	0	1	189	1	189	4.30E-89	337.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97791.t1	Q3EAE5	33.2	223	136	7	21	234	2	220	7.5e-18	92.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97791.t1	A0A396JSM7	89.9	189	19	0	1	189	1	189	3.1e-89	337.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene97791	MS.gene024262	PPI
MS.gene97791	MS.gene67914	PPI
MS.gene97791	MS.gene60522	PPI
MS.gene97791	MS.gene80861	PPI
MS.gene97791	MS.gene026015	PPI
MS.gene97791	MS.gene028491	PPI
MS.gene97791	MS.gene36942	PPI
MS.gene42267	MS.gene97791	PPI
MS.gene97791	MS.gene51793	PPI
MS.gene67917	MS.gene97791	PPI
MS.gene047137	MS.gene97791	PPI
MS.gene97791	MS.gene072244	PPI
MS.gene97791	MS.gene033694	PPI
MS.gene97791	MS.gene045772	PPI
MS.gene97791	MS.gene007794	PPI
MS.gene97791	MS.gene54960	PPI
MS.gene97791	MS.gene90228	PPI
MS.gene97791	MS.gene30044	PPI
MS.gene040253	MS.gene97791	PPI
MS.gene055754	MS.gene97791	PPI
MS.gene97791	MS.gene09993	PPI
MS.gene32300	MS.gene97791	PPI
MS.gene97791	MS.gene072003	PPI
MS.gene97791	MS.gene002112	PPI
MS.gene97791	MS.gene047136	PPI
MS.gene97791	MS.gene36752	PPI
MS.gene36764	MS.gene97791	PPI
MS.gene36942	MS.gene97791	PPI
MS.gene09993	MS.gene97791	PPI
MS.gene97791	MS.gene065373	PPI
MS.gene054896	MS.gene97791	PPI
MS.gene97791	MS.gene033695	PPI
MS.gene36804	MS.gene97791	PPI
MS.gene97791	MS.gene60520	PPI
MS.gene97791	MS.gene068017	PPI
MS.gene97791	MS.gene30261	PPI
MS.gene97791	MS.gene54959	PPI
MS.gene97791	MS.gene51795	PPI
MS.gene97791	MS.gene055754	PPI
MS.gene97791	MS.gene30057	PPI
MS.gene97791	MS.gene071257	PPI
MS.gene97791	MS.gene072231	PPI
MS.gene97791	MS.gene035057	PPI
MS.gene97791	MS.gene054896	PPI
MS.gene97791	MS.gene36807	PPI
MS.gene97791	MS.gene36743	PPI
MS.gene97791	MS.gene32300	PPI
MS.gene97791	MS.gene072000	PPI
MS.gene68081	MS.gene97791	PPI
MS.gene97791	MS.gene36810	PPI
MS.gene97791	MS.gene23706	PPI
MS.gene97791	MS.gene78164	PPI
MS.gene97791	MS.gene09990	PPI
MS.gene072244	MS.gene97791	PPI
MS.gene053586	MS.gene97791	PPI
MS.gene97791	MS.gene68084	PPI
MS.gene97791	MS.gene60518	PPI
MS.gene97791	MS.gene024564	PPI
MS.gene97791	MS.gene050535	PPI
MS.gene97791	MS.gene36809	PPI
MS.gene97791	MS.gene01653	PPI
MS.gene97791	MS.gene050470	PPI
MS.gene97791	MS.gene36943	PPI
MS.gene32305	MS.gene97791	PPI
MS.gene97791	MS.gene032379	PPI
MS.gene97791	MS.gene67917	PPI
MS.gene97791	MS.gene36763	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97791.t1	MTR_1g090900	87.912	182	22	0	11	192	1	182	1.96e-114	327
MS.gene97791.t1	MTR_1g090883	58.472	301	70	3	1	250	1	297	8.43e-110	321
MS.gene97791.t1	MTR_4g056040	51.562	320	72	4	11	250	1	317	8.46e-90	275
MS.gene97791.t1	MTR_1g038710	54.575	306	80	5	1	250	1	303	3.64e-89	271
MS.gene97791.t1	MTR_4g056030	54.138	290	80	4	11	250	1	287	1.67e-88	270
MS.gene97791.t1	MTR_4g056020	52.759	290	84	4	11	250	1	287	9.41e-85	260
MS.gene97791.t1	MTR_4g056070	48.788	330	76	5	11	250	1	327	3.55e-83	255
MS.gene97791.t1	MTR_4g055580	48.012	327	81	5	10	250	91	414	1.95e-82	258
MS.gene97791.t1	MTR_4g055990	48.318	327	79	5	11	250	1	324	2.34e-82	255
MS.gene97791.t1	MTR_4g055990	48.318	327	79	5	11	250	1	324	3.44e-82	254
MS.gene97791.t1	MTR_4g093690	47.872	282	87	8	3	231	2	276	1.48e-64	202
MS.gene97791.t1	MTR_4g056010	65.625	96	32	1	82	177	3	97	4.01e-33	116
MS.gene97791.t1	MTR_7g110920	46.875	160	61	2	111	250	1	156	2.69e-24	99.4
MS.gene97791.t1	MTR_1g041245	30.672	238	140	5	11	244	1	217	1.28e-17	81.6
MS.gene97791.t1	MTR_7g007080	35.393	178	93	7	19	182	2	171	2.24e-17	80.9
MS.gene97791.t1	MTR_7g033945	33.889	180	98	5	11	177	1	172	4.30e-17	77.0
MS.gene97791.t1	MTR_2g007500	34.211	228	119	9	14	227	7	217	1.94e-16	77.8
MS.gene97791.t1	MTR_2g005990	32.500	200	98	5	21	192	6	196	2.58e-16	77.8
MS.gene97791.t1	MTR_7g034115	33.061	245	140	9	21	249	11	247	6.66e-16	76.6
MS.gene97791.t1	MTR_7g032380	32.512	203	122	5	11	206	1	195	6.98e-16	76.3
MS.gene97791.t1	MTR_1g053025	33.168	202	117	8	21	209	2	198	1.84e-15	75.5
MS.gene97791.t1	MTR_5g084070	35.025	197	117	7	15	209	7	194	6.90e-15	73.9
MS.gene97791.t1	MTR_8g085430	30.159	189	110	5	21	192	11	194	1.68e-14	72.4
MS.gene97791.t1	MTR_7g055943	28.807	243	145	8	21	249	40	268	2.07e-14	72.4
MS.gene97791.t1	MTR_7g098570	33.846	195	115	6	21	209	20	206	3.02e-14	72.0
MS.gene97791.t1	MTR_6g022360	30.672	238	134	9	21	249	43	258	6.56e-14	70.9
MS.gene97791.t1	MTR_7g033915	33.987	153	89	3	11	153	1	151	6.92e-14	70.9
MS.gene97791.t1	MTR_7g098510	32.258	217	122	9	21	227	18	219	7.27e-14	70.9
MS.gene97791.t1	MTR_2g007450	31.651	218	124	9	14	209	9	223	1.41e-13	70.1
MS.gene97791.t1	MTR_7g115270	32.386	176	106	6	20	184	11	184	2.41e-13	69.3
MS.gene97791.t1	MTR_5g073420	33.005	203	119	7	11	203	1	196	3.30e-13	68.9
MS.gene97791.t1	MTR_3g107100	31.068	206	128	8	11	209	1	199	4.41e-13	68.6
MS.gene97791.t1	MTR_7g056447	32.338	201	125	5	14	210	2	195	9.44e-13	67.0
MS.gene97791.t1	MTR_4g098590	33.810	210	120	8	23	217	1	206	1.01e-12	67.4
MS.gene97791.t1	MTR_4g039440	30.493	223	127	11	21	231	34	240	1.25e-12	67.0
MS.gene97791.t1	MTR_3g108150	31.308	214	133	6	20	227	12	217	1.36e-12	67.0
MS.gene97791.t1	MTR_7g098480	33.333	183	114	5	21	200	18	195	1.68e-12	67.0
MS.gene97791.t1	MTR_6g011460	32.065	184	107	7	21	192	46	223	4.48e-12	65.5
MS.gene97791.t1	MTR_8g070950	33.333	183	113	5	18	192	12	193	5.52e-12	65.5
MS.gene97791.t1	MTR_3g108560	31.019	216	134	6	18	227	17	223	7.69e-12	64.3
MS.gene97791.t1	MTR_3g108560	31.019	216	134	6	18	227	17	223	7.86e-12	64.7
MS.gene97791.t1	MTR_8g468840	28.743	167	106	5	20	173	13	179	1.17e-11	64.3
MS.gene97791.t1	MTR_8g445780	27.799	259	136	7	19	229	11	266	1.29e-11	64.3
MS.gene97791.t1	MTR_7g110910	66.000	50	15	1	1	50	148	195	1.68e-11	62.0
MS.gene97791.t1	MTR_7g007370	30.049	203	101	5	7	209	3	164	2.21e-11	63.2
MS.gene97791.t1	MTR_8g014260	27.982	218	134	7	1	202	1	211	3.24e-11	63.2
MS.gene97791.t1	MTR_3g023630	30.256	195	111	4	21	203	8	189	3.42e-11	62.8
MS.gene97791.t1	MTR_1g107385	31.174	247	145	7	20	241	31	277	3.58e-11	62.8
MS.gene97791.t1	MTR_4g024780	28.646	192	127	6	8	192	131	319	4.53e-11	62.8
MS.gene97791.t1	MTR_4g080590	30.178	169	104	5	20	181	22	183	6.36e-11	62.0
MS.gene97791.t1	MTR_7g056287	28.319	226	132	9	21	227	3	217	6.45e-11	62.0
MS.gene97791.t1	MTR_4g080590	30.178	169	104	5	20	181	22	183	6.99e-11	62.0
MS.gene97791.t1	MTR_7g032370	32.075	159	99	4	32	186	1	154	8.00e-11	61.6
MS.gene97791.t1	MTR_7g088260	29.536	237	146	9	16	243	86	310	8.32e-11	62.0
MS.gene97791.t1	MTR_7g017280	27.179	195	131	6	6	192	133	324	9.15e-11	62.0
MS.gene97791.t1	MTR_1g115245	31.765	170	111	2	15	179	20	189	9.22e-11	61.2
MS.gene97791.t1	MTR_1089s0010	28.571	231	135	8	15	228	2	219	9.23e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97791.t1	AT4G14103	32.258	217	136	5	20	230	7	218	6.57e-19	85.1
MS.gene97791.t1	AT4G14103	32.258	217	136	5	20	230	7	218	6.57e-19	85.1
MS.gene97791.t1	AT4G14103	32.273	220	132	6	20	230	7	218	8.08e-19	85.5
MS.gene97791.t1	AT4G00315	33.184	223	136	7	21	234	2	220	1.05e-18	85.1
MS.gene97791.t1	AT1G55660	31.696	224	140	7	20	234	52	271	2.32e-17	80.9
MS.gene97791.t1	AT1G55660	31.839	223	139	7	21	234	53	271	2.41e-17	81.3
MS.gene97791.t1	AT3G59230	33.918	171	98	5	20	176	11	180	4.14e-17	80.5
MS.gene97791.t1	AT5G56420	37.500	160	94	4	20	176	5	161	5.61e-17	80.1
MS.gene97791.t1	AT5G56420	37.500	160	94	4	20	176	5	161	5.61e-17	80.1
MS.gene97791.t1	AT5G56420	37.500	160	94	4	20	176	5	161	5.61e-17	80.1
MS.gene97791.t1	AT5G60610	34.302	172	89	6	21	182	2	159	1.39e-16	78.6
MS.gene97791.t1	AT5G60610	34.302	172	89	6	21	182	2	159	1.39e-16	78.6
MS.gene97791.t1	AT5G56400	33.333	201	126	5	5	202	17	212	1.62e-16	78.6
MS.gene97791.t1	AT4G26340	33.511	188	115	6	21	204	2	183	1.77e-16	78.6
MS.gene97791.t1	AT3G59190	35.260	173	100	4	20	182	11	181	3.27e-16	77.4
MS.gene97791.t1	AT3G59190	35.260	173	100	4	20	182	11	181	5.42e-16	77.4
MS.gene97791.t1	AT3G03030	32.673	202	119	6	21	209	2	199	5.87e-16	76.6
MS.gene97791.t1	AT3G03030	32.673	202	119	6	21	209	2	199	9.34e-16	76.6
MS.gene97791.t1	AT4G10400	33.696	184	113	6	21	200	2	180	3.46e-15	74.7
MS.gene97791.t1	AT4G10400	33.696	184	113	6	21	200	2	180	3.46e-15	74.7
MS.gene97791.t1	AT4G10400	33.696	184	113	6	21	200	2	180	3.46e-15	74.7
MS.gene97791.t1	AT1G51370	30.114	176	106	4	7	177	5	168	6.14e-15	73.6
MS.gene97791.t1	AT4G09920	32.804	189	118	6	21	205	2	185	1.05e-14	72.8
MS.gene97791.t1	AT4G09920	32.804	189	118	6	21	205	2	185	1.05e-14	72.8
MS.gene97791.t1	AT1G51370	30.114	176	106	4	7	177	5	168	1.08e-14	73.2
MS.gene97791.t1	AT4G09920	32.632	190	119	6	20	205	1	185	1.08e-14	72.4
MS.gene97791.t1	AT4G09920	32.632	190	119	6	20	205	1	185	1.08e-14	72.4
MS.gene97791.t1	AT4G09920	32.632	190	119	6	20	205	1	185	1.08e-14	72.4
MS.gene97791.t1	AT3G03040	32.828	198	113	6	21	204	2	193	1.45e-14	73.2
MS.gene97791.t1	AT1G51370	30.114	176	106	4	7	177	5	168	1.48e-14	72.8
MS.gene97791.t1	AT4G14096	27.928	222	149	4	20	235	7	223	1.16e-13	70.5
MS.gene97791.t1	AT4G14096	27.928	222	149	4	20	235	7	223	1.16e-13	70.5
MS.gene97791.t1	AT3G58860	30.556	216	129	6	18	220	4	211	1.18e-13	70.5
MS.gene97791.t1	AT5G38590	33.537	164	104	4	20	181	1	161	1.18e-13	69.3
MS.gene97791.t1	AT5G38392	39.161	143	76	3	21	152	2	144	1.52e-13	68.2
MS.gene97791.t1	AT5G38590	33.742	163	103	4	21	181	2	161	1.90e-13	69.7
MS.gene97791.t1	AT4G15060	29.515	227	150	5	18	238	23	245	2.84e-13	69.3
MS.gene97791.t1	AT4G00320	31.148	183	103	5	17	179	9	188	3.20e-13	69.3
MS.gene97791.t1	AT1G58310	32.432	185	100	4	20	181	7	189	3.96e-13	68.9
MS.gene97791.t1	AT3G59210	34.104	173	104	5	17	181	3	173	4.29e-13	68.9
MS.gene97791.t1	AT3G59210	34.104	173	104	5	17	181	3	173	4.29e-13	68.9
MS.gene97791.t1	AT3G59210	34.104	173	104	5	17	181	3	173	4.29e-13	68.9
MS.gene97791.t1	AT3G59210	34.104	173	104	5	17	181	3	173	4.29e-13	68.9
MS.gene97791.t1	AT3G59210	34.104	173	104	5	17	181	3	173	4.29e-13	68.9
MS.gene97791.t1	AT5G41840	32.184	174	100	4	21	176	14	187	4.44e-13	68.9
MS.gene97791.t1	AT3G50710	28.704	216	124	6	21	208	2	215	4.68e-13	68.6
MS.gene97791.t1	AT3G49150	27.919	197	134	3	19	209	14	208	4.79e-13	68.6
MS.gene97791.t1	AT4G13960	30.952	210	128	6	21	220	2	204	4.86e-13	68.6
MS.gene97791.t1	AT4G13960	30.952	210	128	6	21	220	2	204	4.86e-13	68.6
MS.gene97791.t1	AT5G56370	30.952	168	101	4	21	181	2	161	5.37e-13	67.8
MS.gene97791.t1	AT3G59150	32.243	214	121	8	3	204	2	203	5.78e-13	68.2
MS.gene97791.t1	AT3G59200	30.631	222	131	8	20	227	6	218	6.41e-13	67.8
MS.gene97791.t1	AT5G56370	30.952	168	101	4	21	181	2	161	6.73e-13	68.2
MS.gene97791.t1	AT5G56370	30.952	168	101	4	21	181	2	161	6.73e-13	68.2
MS.gene97791.t1	AT5G41830	30.601	183	104	4	17	179	9	188	7.27e-13	68.2
MS.gene97791.t1	AT3G44810	31.818	198	121	5	23	209	9	203	9.34e-13	67.8
MS.gene97791.t1	AT3G59200	30.631	222	131	8	20	227	6	218	1.47e-12	67.4
MS.gene97791.t1	AT4G15060	29.258	229	152	5	16	238	167	391	1.48e-12	67.4
MS.gene97791.t1	AT5G56380	27.972	286	142	7	21	242	2	287	2.29e-12	66.6
MS.gene97791.t1	AT5G56380	27.972	286	142	7	21	242	2	287	2.29e-12	66.6
MS.gene97791.t1	AT5G56440	32.979	188	119	5	21	205	2	185	2.96e-12	66.2
MS.gene97791.t1	AT4G26350	29.697	165	104	3	21	179	2	160	3.54e-12	65.9
MS.gene97791.t1	AT5G53840	30.918	207	126	7	9	208	3	199	4.19e-12	65.9
MS.gene97791.t1	AT5G38590	29.630	189	126	5	21	204	2	188	4.54e-12	64.7
MS.gene97791.t1	AT2G04230	31.507	219	126	9	20	230	12	214	5.95e-12	65.5
MS.gene97791.t1	AT3G44060	33.161	193	104	8	26	204	4	185	8.23e-12	64.7
MS.gene97791.t1	AT1G66310	29.032	186	118	5	1	181	1	177	8.82e-12	64.7
MS.gene97791.t1	AT5G44490	29.358	218	125	8	1	205	1	202	1.05e-11	64.7
MS.gene97791.t1	AT5G44490	29.358	218	125	8	1	205	1	202	1.10e-11	64.7
MS.gene97791.t1	AT3G59250	31.737	167	86	3	20	186	6	144	1.72e-11	63.9
MS.gene97791.t1	AT5G44950	30.653	199	110	10	20	205	3	186	2.64e-11	63.5
MS.gene97791.t1	AT5G18780	30.769	169	104	5	20	183	10	170	3.30e-11	62.8
MS.gene97791.t1	AT5G18780	30.769	169	104	5	20	183	10	170	3.30e-11	62.8
MS.gene97791.t1	AT5G18780	30.769	169	104	5	20	183	10	170	3.78e-11	62.8
MS.gene97791.t1	AT5G18780	30.769	169	104	5	20	183	10	170	3.78e-11	62.8
MS.gene97791.t1	AT1G66300	27.835	194	120	5	1	181	1	187	4.11e-11	62.8
MS.gene97791.t1	AT3G59160	27.315	216	122	7	20	203	12	224	4.31e-11	62.8
MS.gene97791.t1	AT3G58900	31.429	175	90	6	21	176	2	165	7.21e-11	61.6
MS.gene97791.t1	AT3G58900	31.429	175	90	6	21	176	2	165	7.72e-11	61.6
MS.gene97791.t1	AT3G58900	31.429	175	90	6	21	176	2	165	7.72e-11	61.6
MS.gene97791.t1	AT3G59000	32.759	174	103	4	21	181	2	174	8.74e-11	62.0
MS.gene97791.t1	AT3G58900	31.429	175	90	6	21	176	2	165	9.42e-11	61.6

Find 48 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGATTGCTTTGGACGAAAT+AGG	0.255211	3.4:+17709226	None:intergenic
CAAGGTTGATGAACTTGAAT+TGG	0.267869	3.4:-17709086	MS.gene97791:CDS
GGATTGCTTTGGACGAAATA+GGG	0.347836	3.4:+17709227	None:intergenic
CAATAAGATTGGTTTCTCTA+TGG	0.356459	3.4:+17709054	None:intergenic
TCTTCGCATTTCTAAGCATC+TGG	0.358395	3.4:-17708612	MS.gene97791:CDS
TCAGAAGAAGAATTAATTCC+TGG	0.365045	3.4:+17708971	None:intergenic
GCTCGTGGTCTTGAAATTGC+GGG	0.367607	3.4:-17709005	MS.gene97791:CDS
AAAGTCAAGGTTCGGGATCT+TGG	0.367911	3.4:+17709270	None:intergenic
GGTAATTCAAAGTCAAGGTT+CGG	0.373814	3.4:+17709262	None:intergenic
GAAATAGGGTCTGTAATCTT+TGG	0.384244	3.4:+17709241	None:intergenic
AACCAATCTTATTGCCCTTT+AGG	0.384324	3.4:-17709045	MS.gene97791:CDS
ATATACAGATTGGATTGCTT+TGG	0.388000	3.4:+17709216	None:intergenic
ACCCACAAGTGCATATCATA+TGG	0.391836	3.4:+17709127	None:intergenic
AGCCTAAAGGGCAATAAGAT+TGG	0.392082	3.4:+17709043	None:intergenic
GTAATTCAAAGTCAAGGTTC+GGG	0.405746	3.4:+17709263	None:intergenic
CCCATATGATATGCACTTGT+GGG	0.415753	3.4:-17709128	MS.gene97791:CDS
ATGAACTTGAATTGGATTGT+AGG	0.435987	3.4:-17709078	MS.gene97791:CDS
AAGAGTGGCTCGATCAAGAT+TGG	0.444407	3.4:+17708631	None:intergenic
TTGTAGATAGAACACTTGTT+TGG	0.449162	3.4:+17709311	None:intergenic
GACCACGAGCGACTTAGAAA+TGG	0.449696	3.4:+17709018	None:intergenic
ATCCTGCTCTTCATCAGATT+CGG	0.451545	3.4:+17709402	None:intergenic
GGAGAGCTGCATATACAGAT+TGG	0.456055	3.4:+17709206	None:intergenic
CTTAGAAATGGAAAGCCTAA+AGG	0.485386	3.4:+17709030	None:intergenic
TTAGAAATGGAAAGCCTAAA+GGG	0.487373	3.4:+17709031	None:intergenic
TAGAATTGAACACAATAAGT+TGG	0.489735	3.4:+17708915	None:intergenic
CGCTCGTGGTCTTGAAATTG+CGG	0.490023	3.4:-17709006	MS.gene97791:CDS
CAAGTGTTCTATCTACAAGA+TGG	0.503005	3.4:-17709306	MS.gene97791:CDS
TTGCGGGGCGGAACTATGCC+AGG	0.505023	3.4:-17708989	MS.gene97791:CDS
CTCCGAATCTGATGAAGAGC+AGG	0.514545	3.4:-17709404	MS.gene97791:CDS
CCCACAAGTGCATATCATAT+GGG	0.516903	3.4:+17709128	None:intergenic
TTCAAAAGCATTAGACCTCA+AGG	0.530430	3.4:-17709104	MS.gene97791:CDS
TATGAAGAACTTCAAGTGAC+GGG	0.538915	3.4:+17708702	None:intergenic
CCCCATATGATATGCACTTG+TGG	0.550566	3.4:-17709129	MS.gene97791:CDS
TTCCATTTCTAAGTCGCTCG+TGG	0.557905	3.4:-17709020	MS.gene97791:CDS
CTAAGTCTCTATAGTGACAA+TGG	0.561315	3.4:-17709157	MS.gene97791:CDS
AGTTTGTATTCACTCTTGAG+AGG	0.563248	3.4:-17708581	MS.gene97791:CDS
AAGTGTTCTATCTACAAGAT+GGG	0.565572	3.4:-17709305	MS.gene97791:CDS
GACGGGGCATTAATTTGCAG+TGG	0.587492	3.4:+17708719	None:intergenic
TCTTTGGTAATTCAAAGTCA+AGG	0.592329	3.4:+17709257	None:intergenic
ATTCAAGTTCATCAACCTTG+AGG	0.596057	3.4:+17709089	None:intergenic
AGATGAAGAATCTTTAGTGA+CGG	0.601943	3.4:+17708938	None:intergenic
ATATGAAGAACTTCAAGTGA+CGG	0.613196	3.4:+17708701	None:intergenic
CTCGTGGTCTTGAAATTGCG+GGG	0.626254	3.4:-17709004	MS.gene97791:CDS
ATGCTTAGAAATGCGAAGAG+TGG	0.630789	3.4:+17708616	None:intergenic
TATTGTGTTCAATTCTATCG+CGG	0.635341	3.4:-17708909	MS.gene97791:CDS
CCACAAGTGCATATCATATG+GGG	0.653247	3.4:+17709129	None:intergenic
ATGAAGAACTTCAAGTGACG+GGG	0.714715	3.4:+17708703	None:intergenic
GTGGTCTTGAAATTGCGGGG+CGG	0.723470	3.4:-17709001	MS.gene97791:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTGTTAATTCATATTTTCT+TGG	+	chr3.4:17709345-17709364	None:intergenic	15.0%
!	TAGAATTGAACACAATAAGT+TGG	+	chr3.4:17709089-17709108	None:intergenic	25.0%
	AAAACAAATTCCTCAAGATG+TGG	+	chr3.4:17709162-17709181	None:intergenic	30.0%
	AAGTGTTCTATCTACAAGAT+GGG	-	chr3.4:17708696-17708715	MS.gene97791:CDS	30.0%
	AATGAACACTCTCTTTAATG+AGG	+	chr3.4:17709226-17709245	None:intergenic	30.0%
	AGATGAAGAATCTTTAGTGA+CGG	+	chr3.4:17709066-17709085	None:intergenic	30.0%
	ATATACAGATTGGATTGCTT+TGG	+	chr3.4:17708788-17708807	None:intergenic	30.0%
	ATATGAAGAACTTCAAGTGA+CGG	+	chr3.4:17709303-17709322	None:intergenic	30.0%
	ATGAACACTCTCTTTAATGA+GGG	+	chr3.4:17709225-17709244	None:intergenic	30.0%
	ATGAACTTGAATTGGATTGT+AGG	-	chr3.4:17708923-17708942	MS.gene97791:CDS	30.0%
	CAACAAGAAACCAAAAACAA+TGG	-	chr3.4:17708570-17708589	MS.gene97791:CDS	30.0%
	TATTGTGTTCAATTCTATCG+CGG	-	chr3.4:17709092-17709111	MS.gene97791:CDS	30.0%
	TCAGAAGAAGAATTAATTCC+TGG	+	chr3.4:17709033-17709052	None:intergenic	30.0%
	TTAGAAATGGAAAGCCTAAA+GGG	+	chr3.4:17708973-17708992	None:intergenic	30.0%
	TTGTAGATAGAACACTTGTT+TGG	+	chr3.4:17708693-17708712	None:intergenic	30.0%
!	TCTTTGGTAATTCAAAGTCA+AGG	+	chr3.4:17708747-17708766	None:intergenic	30.0%
!!	CAATAAGATTGGTTTCTCTA+TGG	+	chr3.4:17708950-17708969	None:intergenic	30.0%
!!!	TTAAGTTTTTAGTGCGTCTT+AGG	+	chr3.4:17708819-17708838	None:intergenic	30.0%
	AACCAATCTTATTGCCCTTT+AGG	-	chr3.4:17708956-17708975	MS.gene97791:CDS	35.0%
	AGTTTGTATTCACTCTTGAG+AGG	-	chr3.4:17709420-17709439	MS.gene97791:CDS	35.0%
	CAAGTGTTCTATCTACAAGA+TGG	-	chr3.4:17708695-17708714	MS.gene97791:CDS	35.0%
	CTAAGTCTCTATAGTGACAA+TGG	-	chr3.4:17708844-17708863	MS.gene97791:intron	35.0%
	CTTAGAAATGGAAAGCCTAA+AGG	+	chr3.4:17708974-17708993	None:intergenic	35.0%
	GAAATAGGGTCTGTAATCTT+TGG	+	chr3.4:17708763-17708782	None:intergenic	35.0%
	GGTAATTCAAAGTCAAGGTT+CGG	+	chr3.4:17708742-17708761	None:intergenic	35.0%
	GTAATTCAAAGTCAAGGTTC+GGG	+	chr3.4:17708741-17708760	None:intergenic	35.0%
	TATGAAGAACTTCAAGTGAC+GGG	+	chr3.4:17709302-17709321	None:intergenic	35.0%
	TGTTTATTTATGCCTGTTGC+TGG	-	chr3.4:17709246-17709265	MS.gene97791:CDS	35.0%
!	ATTCAAGTTCATCAACCTTG+AGG	+	chr3.4:17708915-17708934	None:intergenic	35.0%
!!	CAAGGTTGATGAACTTGAAT+TGG	-	chr3.4:17708915-17708934	MS.gene97791:CDS	35.0%
!!	TTCAAAAGCATTAGACCTCA+AGG	-	chr3.4:17708897-17708916	MS.gene97791:intron	35.0%
!!!	GGTAAGACTCTTTTGTTGTT+AGG	+	chr3.4:17708672-17708691	None:intergenic	35.0%
	AATTTGCAGTGGACACACTT+TGG	+	chr3.4:17709274-17709293	None:intergenic	40.0%
	ACCCACAAGTGCATATCATA+TGG	+	chr3.4:17708877-17708896	None:intergenic	40.0%
	AGCCTAAAGGGCAATAAGAT+TGG	+	chr3.4:17708961-17708980	None:intergenic	40.0%
	ATCCTGCTCTTCATCAGATT+CGG	+	chr3.4:17708602-17708621	None:intergenic	40.0%
	ATGAAGAACTTCAAGTGACG+GGG	+	chr3.4:17709301-17709320	None:intergenic	40.0%
	ATGCTTAGAAATGCGAAGAG+TGG	+	chr3.4:17709388-17709407	None:intergenic	40.0%
	CCACAAGTGCATATCATATG+GGG	+	chr3.4:17708875-17708894	None:intergenic	40.0%
	CCCACAAGTGCATATCATAT+GGG	+	chr3.4:17708876-17708895	None:intergenic	40.0%
	CCCATATGATATGCACTTGT+GGG	-	chr3.4:17708873-17708892	MS.gene97791:intron	40.0%
	TCTTCGCATTTCTAAGCATC+TGG	-	chr3.4:17709389-17709408	MS.gene97791:CDS	40.0%
!	GGATTGCTTTGGACGAAATA+GGG	+	chr3.4:17708777-17708796	None:intergenic	40.0%
!	TGGATTGCTTTGGACGAAAT+AGG	+	chr3.4:17708778-17708797	None:intergenic	40.0%
	AAAGTCAAGGTTCGGGATCT+TGG	+	chr3.4:17708734-17708753	None:intergenic	45.0%
	AAGAGTGGCTCGATCAAGAT+TGG	+	chr3.4:17709373-17709392	None:intergenic	45.0%
	ATCTCGTTGTCCACATCTTG+AGG	-	chr3.4:17709149-17709168	MS.gene97791:CDS	45.0%
	CCCCATATGATATGCACTTG+TGG	-	chr3.4:17708872-17708891	MS.gene97791:intron	45.0%
	GGAGAGCTGCATATACAGAT+TGG	+	chr3.4:17708798-17708817	None:intergenic	45.0%
	TTCCATTTCTAAGTCGCTCG+TGG	-	chr3.4:17708981-17709000	MS.gene97791:CDS	45.0%
	CTCCGAATCTGATGAAGAGC+AGG	-	chr3.4:17708597-17708616	MS.gene97791:CDS	50.0%
	GACCACGAGCGACTTAGAAA+TGG	+	chr3.4:17708986-17709005	None:intergenic	50.0%
!	CGCTCGTGGTCTTGAAATTG+CGG	-	chr3.4:17708995-17709014	MS.gene97791:CDS	50.0%
!	CTCGTGGTCTTGAAATTGCG+GGG	-	chr3.4:17708997-17709016	MS.gene97791:CDS	50.0%
!	GCTCGTGGTCTTGAAATTGC+GGG	-	chr3.4:17708996-17709015	MS.gene97791:CDS	50.0%
!	TCGGAGCACGCCATTGTTTT+TGG	+	chr3.4:17708583-17708602	None:intergenic	50.0%
!!	CACACTTTGGAACCAGCAAC+AGG	+	chr3.4:17709261-17709280	None:intergenic	50.0%
!!	GACGGGGCATTAATTTGCAG+TGG	+	chr3.4:17709285-17709304	None:intergenic	50.0%
!	GTGGTCTTGAAATTGCGGGG+CGG	-	chr3.4:17709000-17709019	MS.gene97791:CDS	55.0%
	TTGCGGGGCGGAACTATGCC+AGG	-	chr3.4:17709012-17709031	MS.gene97791:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	17708559	17709464	17708559	ID=MS.gene97791
chr3.4	mRNA	17708559	17709464	17708559	ID=MS.gene97791.t1;Parent=MS.gene97791
chr3.4	exon	17708900	17709464	17708900	ID=MS.gene97791.t1.exon1;Parent=MS.gene97791.t1
chr3.4	CDS	17708900	17709464	17708900	ID=cds.MS.gene97791.t1;Parent=MS.gene97791.t1
chr3.4	exon	17708559	17708746	17708559	ID=MS.gene97791.t1.exon2;Parent=MS.gene97791.t1
chr3.4	CDS	17708559	17708746	17708559	ID=cds.MS.gene97791.t1;Parent=MS.gene97791.t1

Gene Sequence

>MS.gene97791

ATGGAGGAAGACAACAAGAAACCAAAAACAATGGCGTGCTCCGAATCTGATGAAGAGCAGGATCGATTAAGCAATCTCACAGACGAATTGATTATTCATATTCTGTCTTTCCTAACAACAAAAGAGTCTTACCAAACAAGTGTTCTATCTACAAGATGGGAGTCAATCTGTACCAAGATCCCGAACCTTGACTTTGAATTACCAAAGATTACAGACCCTATTTCGTCCAAAGCAATCCAATCTGTATATGCAGCTCTCCTAAGACGCACTAAAAACTTAAGAAAACTAAGTCTCTATAGTGACAATGGTTGCACCCCATATGATATGCACTTGTGGGTTTCAAAAGCATTAGACCTCAAGGTTGATGAACTTGAATTGGATTGTAGGTCCATAGAGAAACCAATCTTATTGCCCTTTAGGCTTTCCATTTCTAAGTCGCTCGTGGTCTTGAAATTGCGGGGCGGAACTATGCCAGGAATTAATTCTTCTTCTGATGTGTGTTTTCCGTCACTAAAGATTCTTCATCTCCAACTTATTGTGTTCAATTCTATCGCGGATGATCATAGTGAGTATGATCTCGCTAATTTTCTATCTCGTTGTCCACATCTTGAGGAATTTGTTTTACATGACAACATAAAACAAGCAATCAATATTGTCTCTTTTCCCTCATTAAAGAGAGTGTTCATTTGTTTATTTATGCCTGTTGCTGGTTCCAAAGTGTGTCCACTGCAAATTAATGCCCCGTCACTTGAAGTTCTTCATATTGTTGATGCTTCATTTTCTCCAAGAAAATATGAATTAACAAACTTGTCCAATCTTGATCGAGCCACTCTTCGCATTTCTAAGCATCTGGATTTTAACAGTTTGTATTCACTCTTGAGAGGACTTTATAATGTTAAATCATAG

Protein sequence

>MS.gene97791.t1

MEEDNKKPKTMACSESDEEQDRLSNLTDELIIHILSFLTTKESYQTSVLSTRWESICTKIPNLDFELPKITDPISSKAIQSVYAALLRRTKNLRKLSLYSDNGCTPYDMHLWVSKALDLKVDELELDCRSIEKPILLPFRLSISKSLVVLKLRGGTMPGINSSSDVCFPSLKILHLQLIVFNSIADDHMCPLQINAPSLEVLHIVDASFSPRKYELTNLSNLDRATLRISKHLDFNSLYSLLRGLYNVKS