AlfalfaGEDB | MS.gene049030

M. sativa cultivar XinJiangDaYe / MS.gene049030

KEGG: K02910
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01198.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049030.t1	XP_003630662.2	100	121	0	0	1	121	1	121	5.00E-59	236.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049030.t1	P46290	87.6	121	14	1	1	121	1	120	8.6e-52	204.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049030.t1	B7FGL4	100.0	121	0	0	1	121	1	121	3.6e-59	236.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene049030	MS.gene20153	PPI
MS.gene049030	MS.gene99599	PPI
MS.gene049030	MS.gene08259	PPI
MS.gene049030	MS.gene92970	PPI
MS.gene068781	MS.gene049030	PPI
MS.gene049030	MS.gene96312	PPI
MS.gene049030	MS.gene06169	PPI
MS.gene059846	MS.gene049030	PPI
MS.gene049030	MS.gene09187	PPI
MS.gene049030	MS.gene66352	PPI
MS.gene049030	MS.gene012333	PPI
MS.gene049030	MS.gene029547	PPI
MS.gene049030	MS.gene55890	PPI
MS.gene049030	MS.gene96780	PPI
MS.gene049030	MS.gene023381	PPI
MS.gene049030	MS.gene064028	PPI
MS.gene049030	MS.gene030304	PPI
MS.gene049030	MS.gene75054	PPI
MS.gene049030	MS.gene09046	PPI
MS.gene049030	MS.gene57660	PPI
MS.gene049030	MS.gene065189	PPI
MS.gene049030	MS.gene031406	PPI
MS.gene049030	MS.gene56525	PPI
MS.gene06169	MS.gene049030	PPI
MS.gene049030	MS.gene031484	PPI
MS.gene049030	MS.gene09188	PPI
MS.gene049030	MS.gene073324	PPI
MS.gene049030	MS.gene08260	PPI
MS.gene049030	MS.gene053790	PPI
MS.gene049030	MS.gene065652	PPI
MS.gene006800	MS.gene049030	PPI
MS.gene049030	MS.gene012331	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049030.t1	MTR_8g101980	100.000	121	0	0	1	121	1	121	5.31e-85	243
MS.gene049030.t1	MTR_6g091630	97.521	121	3	0	1	121	1	121	8.29e-83	238

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049030.t1	AT5G56710	83.333	114	19	0	8	121	6	119	5.84e-67	197
MS.gene049030.t1	AT4G26230	82.456	114	20	0	8	121	6	119	2.06e-66	196
MS.gene049030.t1	AT2G19740	81.897	116	21	0	6	121	4	119	2.90e-66	196
MS.gene049030.t1	AT5G56710	58.772	114	13	1	8	121	6	85	6.30e-38	123

Find 50 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGATCTCCTTTATTGCTTT+AGG	0.186329	4.1:+3421834	None:intergenic
TCAAGTTGAACAAGGCTATT+TGG	0.242228	4.1:-3421764	MS.gene049030:CDS
CATTAGTTCCCATAGCCTTT+TGG	0.252576	4.1:+3421802	None:intergenic
CAATCCTTTGAGCTCTTCCT+TGG	0.276372	4.1:+3421626	None:intergenic
GAAAGCTCCTAAAGCAATAA+AGG	0.311659	4.1:-3421841	MS.gene049030:CDS
CTCATCATCGTTCCTCTTGC+GGG	0.315756	4.1:+3421695	None:intergenic
AATCCTTTGAGCTCTTCCTT+GGG	0.346185	4.1:+3421627	None:intergenic
GAAGTGTCCCTAGGAGGGTT+AGG	0.392774	4.1:-3421728	MS.gene049030:CDS
GAGGCGTTTGTGGAGGTTGA+TGG	0.411978	4.1:+3422514	None:intergenic
GATCAGGAAGTTTGCCCAAA+AGG	0.422618	4.1:-3421817	MS.gene049030:CDS
ATGCGAACCCTAACCCTCCT+AGG	0.432434	4.1:+3421720	None:intergenic
CTAAAGCAATAAAGGAGATC+AGG	0.445899	4.1:-3421833	MS.gene049030:CDS
AAGGAAGAGCTCAAAGGATT+GGG	0.447041	4.1:-3421624	MS.gene049030:CDS
ACCAGCCATGGAGGCGTTTG+TGG	0.449631	4.1:+3422504	None:intergenic
CAAGGAAGAGCTCAAAGGAT+TGG	0.452186	4.1:-3421625	MS.gene049030:CDS
CCTCATCATCGTTCCTCTTG+CGG	0.459607	4.1:+3421694	None:intergenic
GAACAAGGCTATTTGGAGCC+AGG	0.460263	4.1:-3421757	MS.gene049030:CDS
TCCACAAACGCCTCCATGGC+TGG	0.473615	4.1:-3422505	MS.gene049030:intron
TAGGGACACTTCTGATCCCC+TGG	0.478265	4.1:+3421739	None:intergenic
CTCTTCCTTCCTACCGGCGC+CGG	0.482937	4.1:+3422556	None:intergenic
GAAGTTTGCCCAAAAGGCTA+TGG	0.483900	4.1:-3421811	MS.gene049030:CDS
GTGAAAATGGTGGAGAAGAC+CGG	0.499433	4.1:-3422575	None:intergenic
GAGGTTGATGGTGTACTCTC+TGG	0.499941	4.1:+3422526	None:intergenic
AAGTGTCCCTAGGAGGGTTA+GGG	0.512529	4.1:-3421727	MS.gene049030:CDS
ATCCTTTGAGCTCTTCCTTG+GGG	0.520497	4.1:+3421628	None:intergenic
AACAAGGCTATTTGGAGCCA+GGG	0.528204	4.1:-3421756	MS.gene049030:CDS
AGAGAGAGAATTACCAGCCA+TGG	0.536558	4.1:+3422492	None:intergenic
CACAAAGGTTATTGATGATG+AGG	0.542190	4.1:-3421601	MS.gene049030:CDS
GAACGATGATGAGGATGCTA+AGG	0.543980	4.1:-3421685	MS.gene049030:CDS
GAAGACCGGCGCCGGTAGGA+AGG	0.546764	4.1:-3422561	MS.gene049030:CDS
CGGCGCCGGTAGGAAGGAAG+AGG	0.547239	4.1:-3422555	MS.gene049030:CDS
AACCTCCACAAACGCCTCCA+TGG	0.560698	4.1:-3422509	MS.gene049030:CDS
AGGGGATCAGAAGTGTCCCT+AGG	0.578761	4.1:-3421737	MS.gene049030:CDS
GACAACCTCTTCCTTCCTAC+CGG	0.582542	4.1:+3422550	None:intergenic
AAGTTTGCCCAAAAGGCTAT+GGG	0.584767	4.1:-3421810	MS.gene049030:CDS
AGCCATGGAGGCGTTTGTGG+AGG	0.589507	4.1:+3422507	None:intergenic
TACTGTTGTTGAAATCCCCA+AGG	0.603575	4.1:-3421643	MS.gene049030:CDS
CCGCAAGAGGAACGATGATG+AGG	0.607958	4.1:-3421694	MS.gene049030:CDS
TGGAGAAGACCGGCGCCGGT+AGG	0.608791	4.1:-3422565	MS.gene049030:CDS
GATCAGAAGTGTCCCTAGGA+GGG	0.609828	4.1:-3421733	MS.gene049030:CDS
ATGGTGGAGAAGACCGGCGC+CGG	0.610511	4.1:-3422569	MS.gene049030:CDS
TGCGAACCCTAACCCTCCTA+GGG	0.611019	4.1:+3421721	None:intergenic
GGGTTCGCATTGCCCGCAAG+AGG	0.617342	4.1:-3421707	MS.gene049030:CDS
GGATCAGAAGTGTCCCTAGG+AGG	0.618801	4.1:-3421734	MS.gene049030:CDS
ATCCCCAAGGAAGAGCTCAA+AGG	0.635127	4.1:-3421630	MS.gene049030:CDS
GAGAGAATTACCAGCCATGG+AGG	0.648989	4.1:+3422495	None:intergenic
ACAAGGCTATTTGGAGCCAG+GGG	0.649532	4.1:-3421755	MS.gene049030:CDS
GCTCAAAGGATTGGGCACAA+AGG	0.653987	4.1:-3421616	MS.gene049030:CDS
AGTGGACGTCAAGTTGAACA+AGG	0.683327	4.1:-3421772	MS.gene049030:CDS
GGGAACTAATGATGTCAGAG+TGG	0.705766	4.1:-3421790	MS.gene049030:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTTTTAATCTCTTTAGA+AGG	-	chr4.1:3421803-3421822	MS.gene049030:CDS	15.0%
!!	ATCAGTTAAAATAACATCTA+TGG	+	chr4.1:3422029-3422048	None:intergenic	20.0%
!!!	TTACAAATATTTTGATAACC+CGG	-	chr4.1:3422049-3422068	MS.gene049030:intron	20.0%
!	ATATGTAAAATAATCCATCC+AGG	+	chr4.1:3422132-3422151	None:intergenic	25.0%
!	CAACATTAATATCAAAGACA+CGG	+	chr4.1:3421911-3421930	None:intergenic	25.0%
!	TAAATAGCATATCACAGAAA+TGG	-	chr4.1:3421972-3421991	MS.gene049030:intron	25.0%
!!	AAACAAAACATTTGCTAGAT+AGG	+	chr4.1:3421733-3421752	None:intergenic	25.0%
!!	AACAAAACATTTGCTAGATA+GGG	+	chr4.1:3421732-3421751	None:intergenic	25.0%
!!	CATCAAAATGAAAAGTACAA+GGG	+	chr4.1:3421853-3421872	None:intergenic	25.0%
!!	GATATAGAGGAAAATTTTTG+TGG	-	chr4.1:3421764-3421783	MS.gene049030:CDS	25.0%
!!	TCATCAAAATGAAAAGTACA+AGG	+	chr4.1:3421854-3421873	None:intergenic	25.0%
!!!	TACAAATATTTTGATAACCC+GGG	-	chr4.1:3422050-3422069	MS.gene049030:intron	25.0%
!!!	TCTTTAGAAGGTTTTTACAA+TGG	-	chr4.1:3421815-3421834	MS.gene049030:CDS	25.0%
	AAAATAATCCATCCAGGTAA+AGG	+	chr4.1:3422126-3422145	None:intergenic	30.0%
	AAATAATCCATCCAGGTAAA+GGG	+	chr4.1:3422125-3422144	None:intergenic	30.0%
!	CTTTTCTATGGAGATTGTTT+CGG	-	chr4.1:3422205-3422224	MS.gene049030:intron	30.0%
!	TTGTTTTCGATCAGATATAG+AGG	-	chr4.1:3421751-3421770	MS.gene049030:CDS	30.0%
!!	GCTGTTTTGCATAATAACAA+AGG	+	chr4.1:3422293-3422312	None:intergenic	30.0%
	AATAATCCATCCAGGTAAAG+GGG	+	chr4.1:3422124-3422143	None:intergenic	35.0%
	ATAGCATATCACAGAAATGG+TGG	-	chr4.1:3421975-3421994	MS.gene049030:intron	35.0%
	CTAAAGCAATAAAGGAGATC+AGG	-	chr4.1:3422332-3422351	MS.gene049030:intron	35.0%
	CTGATCTCCTTTATTGCTTT+AGG	+	chr4.1:3422334-3422353	None:intergenic	35.0%
	GAAAGCTCCTAAAGCAATAA+AGG	-	chr4.1:3422324-3422343	MS.gene049030:intron	35.0%
!	ATTAGTTCCCATAGCCTTTT+GGG	+	chr4.1:3422365-3422384	None:intergenic	35.0%
!	CGTGTGAAATGACTTTTCTA+TGG	-	chr4.1:3422193-3422212	MS.gene049030:intron	35.0%
!	TGCATTTGTCTCAGTTTTTG+TGG	-	chr4.1:3422165-3422184	MS.gene049030:intron	35.0%
!!	CACAAAGGTTATTGATGATG+AGG	-	chr4.1:3422564-3422583	MS.gene049030:CDS	35.0%
!!	TCAAGTTGAACAAGGCTATT+TGG	-	chr4.1:3422401-3422420	MS.gene049030:intron	35.0%
!!!	AAATATTTTGATAACCCGGG+AGG	-	chr4.1:3422053-3422072	MS.gene049030:intron	35.0%
	AAAACTATATTCCTCCTCCC+GGG	+	chr4.1:3422070-3422089	None:intergenic	40.0%
	AAGGAAGAGCTCAAAGGATT+GGG	-	chr4.1:3422541-3422560	MS.gene049030:CDS	40.0%
	AAGTTTGCCCAAAAGGCTAT+GGG	-	chr4.1:3422355-3422374	MS.gene049030:intron	40.0%
	AATCCTTTGAGCTCTTCCTT+GGG	+	chr4.1:3422541-3422560	None:intergenic	40.0%
	ACAAACATATCCCCTTTACC+TGG	-	chr4.1:3422111-3422130	MS.gene049030:intron	40.0%
	CAAAACTATATTCCTCCTCC+CGG	+	chr4.1:3422071-3422090	None:intergenic	40.0%
	TACTGTTGTTGAAATCCCCA+AGG	-	chr4.1:3422522-3422541	MS.gene049030:CDS	40.0%
!	CATTAGTTCCCATAGCCTTT+TGG	+	chr4.1:3422366-3422385	None:intergenic	40.0%
	ACATATCCCCTTTACCTGGA+TGG	-	chr4.1:3422115-3422134	MS.gene049030:intron	45.0%
	AGAGAGAGAATTACCAGCCA+TGG	+	chr4.1:3421676-3421695	None:intergenic	45.0%
	ATCCTTTGAGCTCTTCCTTG+GGG	+	chr4.1:3422540-3422559	None:intergenic	45.0%
	CAAGGAAGAGCTCAAAGGAT+TGG	-	chr4.1:3422540-3422559	MS.gene049030:CDS	45.0%
	CAATCCTTTGAGCTCTTCCT+TGG	+	chr4.1:3422542-3422561	None:intergenic	45.0%
	GAACGATGATGAGGATGCTA+AGG	-	chr4.1:3422480-3422499	MS.gene049030:intron	45.0%
	GAAGTTTGCCCAAAAGGCTA+TGG	-	chr4.1:3422354-3422373	MS.gene049030:intron	45.0%
	GATCAGGAAGTTTGCCCAAA+AGG	-	chr4.1:3422348-3422367	MS.gene049030:intron	45.0%
	GGGAACTAATGATGTCAGAG+TGG	-	chr4.1:3422375-3422394	MS.gene049030:intron	45.0%
!	AACAAGGCTATTTGGAGCCA+GGG	-	chr4.1:3422409-3422428	MS.gene049030:intron	45.0%
!!	AGTGGACGTCAAGTTGAACA+AGG	-	chr4.1:3422393-3422412	MS.gene049030:intron	45.0%
!!!	TATTTTGATAACCCGGGAGG+AGG	-	chr4.1:3422056-3422075	MS.gene049030:intron	45.0%
	AAGTGTCCCTAGGAGGGTTA+GGG	-	chr4.1:3422438-3422457	MS.gene049030:intron	50.0%
	ATCCCCAAGGAAGAGCTCAA+AGG	-	chr4.1:3422535-3422554	MS.gene049030:CDS	50.0%
	CCTCATCATCGTTCCTCTTG+CGG	+	chr4.1:3422474-3422493	None:intergenic	50.0%
	CTCATCATCGTTCCTCTTGC+GGG	+	chr4.1:3422473-3422492	None:intergenic	50.0%
	GACAACCTCTTCCTTCCTAC+CGG	+	chr4.1:3421618-3421637	None:intergenic	50.0%
	GAGAGAATTACCAGCCATGG+AGG	+	chr4.1:3421673-3421692	None:intergenic	50.0%
	GCTCAAAGGATTGGGCACAA+AGG	-	chr4.1:3422549-3422568	MS.gene049030:CDS	50.0%
!	ACAAGGCTATTTGGAGCCAG+GGG	-	chr4.1:3422410-3422429	MS.gene049030:intron	50.0%
!	GAACAAGGCTATTTGGAGCC+AGG	-	chr4.1:3422408-3422427	MS.gene049030:intron	50.0%
!!	GAGGTTGATGGTGTACTCTC+TGG	+	chr4.1:3421642-3421661	None:intergenic	50.0%
!!	GATCAGAAGTGTCCCTAGGA+GGG	-	chr4.1:3422432-3422451	MS.gene049030:intron	50.0%
	AACCTCCACAAACGCCTCCA+TGG	-	chr4.1:3421656-3421675	MS.gene049030:CDS	55.0%
	ATGCGAACCCTAACCCTCCT+AGG	+	chr4.1:3422448-3422467	None:intergenic	55.0%
	CCGCAAGAGGAACGATGATG+AGG	-	chr4.1:3422471-3422490	MS.gene049030:intron	55.0%
	TAGGGACACTTCTGATCCCC+TGG	+	chr4.1:3422429-3422448	None:intergenic	55.0%
	TGCGAACCCTAACCCTCCTA+GGG	+	chr4.1:3422447-3422466	None:intergenic	55.0%
!	GAAGTGTCCCTAGGAGGGTT+AGG	-	chr4.1:3422437-3422456	MS.gene049030:intron	55.0%
!	GAGGCGTTTGTGGAGGTTGA+TGG	+	chr4.1:3421654-3421673	None:intergenic	55.0%
!!	AGGGGATCAGAAGTGTCCCT+AGG	-	chr4.1:3422428-3422447	MS.gene049030:intron	55.0%
!!	GGATCAGAAGTGTCCCTAGG+AGG	-	chr4.1:3422431-3422450	MS.gene049030:intron	55.0%
	TCCACAAACGCCTCCATGGC+TGG	-	chr4.1:3421660-3421679	MS.gene049030:CDS	60.0%
!!	ACCAGCCATGGAGGCGTTTG+TGG	+	chr4.1:3421664-3421683	None:intergenic	60.0%
!!	AGCCATGGAGGCGTTTGTGG+AGG	+	chr4.1:3421661-3421680	None:intergenic	60.0%
	CTCTTCCTTCCTACCGGCGC+CGG	+	chr4.1:3421612-3421631	None:intergenic	65.0%
	GGGTTCGCATTGCCCGCAAG+AGG	-	chr4.1:3422458-3422477	MS.gene049030:intron	65.0%
!	ATGGTGGAGAAGACCGGCGC+CGG	-	chr4.1:3421596-3421615	MS.gene049030:CDS	65.0%
!!	CGGCGCCGGTAGGAAGGAAG+AGG	-	chr4.1:3421610-3421629	MS.gene049030:CDS	70.0%
!!	GAAGACCGGCGCCGGTAGGA+AGG	-	chr4.1:3421604-3421623	MS.gene049030:CDS	70.0%
!!	TGGAGAAGACCGGCGCCGGT+AGG	-	chr4.1:3421600-3421619	MS.gene049030:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	3421596	3422591	3421596	ID=MS.gene049030
chr4.1	mRNA	3421596	3422591	3421596	ID=MS.gene049030.t1;Parent=MS.gene049030
chr4.1	exon	3422506	3422591	3422506	ID=MS.gene049030.t1.exon1;Parent=MS.gene049030.t1
chr4.1	CDS	3422506	3422591	3422506	ID=cds.MS.gene049030.t1;Parent=MS.gene049030.t1
chr4.1	exon	3421596	3421875	3421596	ID=MS.gene049030.t1.exon2;Parent=MS.gene049030.t1
chr4.1	CDS	3421596	3421875	3421596	ID=cds.MS.gene049030.t1;Parent=MS.gene049030.t1

Gene Sequence

>MS.gene049030

ATGGTGGAGAAGACCGGCGCCGGTAGGAAGGAAGAGGTTGTCACCAGAGAGTACACCATCAACCTCCACAAACGCCTCCATGGCTGGTAATTCTCTCTCTATTGTTCATTTTTGTTTTTTTCGTTTGTCTAAACCCTATCTAGCAAATGTTTTGTTTGTTTTCGATCAGATATAGAGGAAAATTTTTGTGGATTTTTTATTTATTATATTTTTTTAATCTCTTTAGAAGGTTTTTACAATGGCAGTAAAATAGTCCCTTGTACTTTTCATTTTGATGATATTGTAATTAAGCTGTTTGAATGTTTAAATGTTCCGTGTCTTTGATATTAATGTTGTCAATTGTGCTATAATGCTGCTAGTTCGCACAATTTTAAGCTAAATAGCATATCACAGAAATGGTGGTTTGCTATGCACTGCGTTTCTAGCAACTCCATAGATGTTATTTTAACTGATTTACAAATATTTTGATAACCCGGGAGGAGGAATATAGTTTTGTGAGACAATACTTTGATGTAACAAACATATCCCCTTTACCTGGATGGATTATTTTACATATTTACAACTACATTTGCATTTGTCTCAGTTTTTGTGGTTTATCGTGTGAAATGACTTTTCTATGGAGATTGTTTCGGCTGATATCTCGTTTGTATTATTTGTTTGTTTTAACTTTATGAAATTCTAACAGTCATTTTTTCCTTTGTTATTATGCAAAACAGCACATTTAAGAAGAAAGCTCCTAAAGCAATAAAGGAGATCAGGAAGTTTGCCCAAAAGGCTATGGGAACTAATGATGTCAGAGTGGACGTCAAGTTGAACAAGGCTATTTGGAGCCAGGGGATCAGAAGTGTCCCTAGGAGGGTTAGGGTTCGCATTGCCCGCAAGAGGAACGATGATGAGGATGCTAAGGAAGAGCTCTACTCACTTGTTACTGTTGTTGAAATCCCCAAGGAAGAGCTCAAAGGATTGGGCACAAAGGTTATTGATGATGAGGATTGA

Protein sequence

>MS.gene049030.t1

MVEKTGAGRKEEVVTREYTINLHKRLHGCTFKKKAPKAIKEIRKFAQKAMGTNDVRVDVKLNKAIWSQGIRSVPRRVRVRIARKRNDDEDAKEELYSLVTVVEIPKEELKGLGTKVIDDED