AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene55890


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55890.t1 MTR_8g101980 100.000 121 0 0 1 121 1 121 5.31e-85 243
MS.gene55890.t1 MTR_6g091630 97.521 121 3 0 1 121 1 121 8.29e-83 238
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55890.t1 AT5G56710 83.333 114 19 0 8 121 6 119 5.84e-67 197
MS.gene55890.t1 AT4G26230 82.456 114 20 0 8 121 6 119 2.06e-66 196
MS.gene55890.t1 AT2G19740 81.897 116 21 0 6 121 4 119 2.90e-66 196
MS.gene55890.t1 AT5G56710 58.772 114 13 1 8 121 6 85 6.30e-38 123

Find 50 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGATCTCCTTTATTGCTTT+AGG 0.186329 4.3:+3455773 None:intergenic
TCAAGTTGAACAAGGCTATT+TGG 0.242228 4.3:-3455703 MS.gene55890:CDS
CATTAGTTCCCATAGCCTTT+TGG 0.252576 4.3:+3455741 None:intergenic
CAATCCTTTGAGCTCTTCCT+TGG 0.276372 4.3:+3455565 None:intergenic
GAAAGCTCCTAAAGCAATAA+AGG 0.311659 4.3:-3455780 MS.gene55890:CDS
CTCATCATCGTTCCTCTTGC+GGG 0.315756 4.3:+3455634 None:intergenic
AATCCTTTGAGCTCTTCCTT+GGG 0.346185 4.3:+3455566 None:intergenic
GAAGTGTCCCTAGGAGGGTT+AGG 0.392774 4.3:-3455667 MS.gene55890:CDS
GAGGCGTTTGTGGAGGTTGA+TGG 0.411978 4.3:+3456383 None:intergenic
GATCAGGAAGTTTGCCCAAA+AGG 0.422618 4.3:-3455756 MS.gene55890:CDS
ATGCGAACCCTAACCCTCCT+AGG 0.432434 4.3:+3455659 None:intergenic
CTAAAGCAATAAAGGAGATC+AGG 0.445899 4.3:-3455772 MS.gene55890:CDS
AAGGAAGAGCTCAAAGGATT+GGG 0.447041 4.3:-3455563 MS.gene55890:CDS
ACCAGCCATGGAGGCGTTTG+TGG 0.449631 4.3:+3456373 None:intergenic
CAAGGAAGAGCTCAAAGGAT+TGG 0.452186 4.3:-3455564 MS.gene55890:CDS
CCTCATCATCGTTCCTCTTG+CGG 0.459607 4.3:+3455633 None:intergenic
GAACAAGGCTATTTGGAGCC+AGG 0.460263 4.3:-3455696 MS.gene55890:CDS
TCCACAAACGCCTCCATGGC+TGG 0.473615 4.3:-3456374 MS.gene55890:intron
TAGGGACACTTCTGATCCCC+TGG 0.478265 4.3:+3455678 None:intergenic
CTCTTCCTTCCTACCGGCGC+CGG 0.482937 4.3:+3456425 None:intergenic
GAAGTTTGCCCAAAAGGCTA+TGG 0.483900 4.3:-3455750 MS.gene55890:CDS
GTGAAAATGGTGGAGAAGAC+CGG 0.499433 4.3:-3456444 None:intergenic
GAGGTTGATGGTGTACTCTC+TGG 0.499941 4.3:+3456395 None:intergenic
AAGTGTCCCTAGGAGGGTTA+GGG 0.512529 4.3:-3455666 MS.gene55890:CDS
ATCCTTTGAGCTCTTCCTTG+GGG 0.520497 4.3:+3455567 None:intergenic
AACAAGGCTATTTGGAGCCA+GGG 0.528204 4.3:-3455695 MS.gene55890:CDS
CACAAAGGTTATTGATGATG+AGG 0.542190 4.3:-3455540 MS.gene55890:CDS
GAACGATGATGAGGATGCTA+AGG 0.543980 4.3:-3455624 MS.gene55890:CDS
GAAGACCGGCGCCGGTAGGA+AGG 0.546764 4.3:-3456430 MS.gene55890:CDS
CGGCGCCGGTAGGAAGGAAG+AGG 0.547239 4.3:-3456424 MS.gene55890:CDS
ATAGAGAGAATTACCAGCCA+TGG 0.558172 4.3:+3456361 None:intergenic
AACCTCCACAAACGCCTCCA+TGG 0.560698 4.3:-3456378 MS.gene55890:CDS
AGGGGATCAGAAGTGTCCCT+AGG 0.578761 4.3:-3455676 MS.gene55890:CDS
GACAACCTCTTCCTTCCTAC+CGG 0.582542 4.3:+3456419 None:intergenic
AAGTTTGCCCAAAAGGCTAT+GGG 0.584767 4.3:-3455749 MS.gene55890:CDS
AGCCATGGAGGCGTTTGTGG+AGG 0.589507 4.3:+3456376 None:intergenic
TACTGTTGTTGAAATCCCCA+AGG 0.603575 4.3:-3455582 MS.gene55890:CDS
CCGCAAGAGGAACGATGATG+AGG 0.607958 4.3:-3455633 MS.gene55890:CDS
TGGAGAAGACCGGCGCCGGT+AGG 0.608791 4.3:-3456434 MS.gene55890:CDS
GATCAGAAGTGTCCCTAGGA+GGG 0.609828 4.3:-3455672 MS.gene55890:CDS
ATGGTGGAGAAGACCGGCGC+CGG 0.610511 4.3:-3456438 MS.gene55890:CDS
TGCGAACCCTAACCCTCCTA+GGG 0.611019 4.3:+3455660 None:intergenic
GGGTTCGCATTGCCCGCAAG+AGG 0.617342 4.3:-3455646 MS.gene55890:CDS
GGATCAGAAGTGTCCCTAGG+AGG 0.618801 4.3:-3455673 MS.gene55890:CDS
ATCCCCAAGGAAGAGCTCAA+AGG 0.635127 4.3:-3455569 MS.gene55890:CDS
ACAAGGCTATTTGGAGCCAG+GGG 0.649532 4.3:-3455694 MS.gene55890:CDS
GCTCAAAGGATTGGGCACAA+AGG 0.653987 4.3:-3455555 MS.gene55890:CDS
GAGAGAATTACCAGCCATGG+AGG 0.681349 4.3:+3456364 None:intergenic
AGTGGACGTCAAGTTGAACA+AGG 0.683327 4.3:-3455711 MS.gene55890:CDS
GGGAACTAATGATGTCAGAG+TGG 0.705766 4.3:-3455729 MS.gene55890:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTTAATCTCTTTAGA+AGG - chr4.3:3455739-3455758 MS.gene55890:CDS 15.0%
!!! TTTACAAATATTTTGATAAC+CGG - chr4.3:3455984-3456003 MS.gene55890:intron 15.0%
!! AAATAATACAAACGAGATAT+CGG + chr4.3:3456100-3456119 None:intergenic 20.0%
!! ATCAGTTAAAATAACATCTA+CGG + chr4.3:3455965-3455984 None:intergenic 20.0%
!!! TTACAAATATTTTGATAACC+GGG - chr4.3:3455985-3456004 MS.gene55890:intron 20.0%
! TAAATAGCATATCACAGAAA+TGG - chr4.3:3455908-3455927 MS.gene55890:intron 25.0%
!! AAACAAAACATTTGCTAGAT+AGG + chr4.3:3455670-3455689 None:intergenic 25.0%
!! AACAAAACATTTGCTAGATA+GGG + chr4.3:3455669-3455688 None:intergenic 25.0%
!! GATATAGAGGAAAATTTTTG+TGG - chr4.3:3455701-3455720 MS.gene55890:CDS 25.0%
!!! TCTTTAGAAGGTTTTTTCAA+TGG - chr4.3:3455751-3455770 MS.gene55890:CDS 25.0%
! AAAAGTCATTTCAGGTAAAG+GGG + chr4.3:3456059-3456078 None:intergenic 30.0%
! AGAAAAGTCATTTCAGGTAA+AGG + chr4.3:3456061-3456080 None:intergenic 30.0%
! CATCAAAATGAAAAGCACAA+GGG + chr4.3:3455789-3455808 None:intergenic 30.0%
! CTTTTCTATGGAGATTGTTT+CGG - chr4.3:3456074-3456093 MS.gene55890:intron 30.0%
! GAAAAGTCATTTCAGGTAAA+GGG + chr4.3:3456060-3456079 None:intergenic 30.0%
! TACCTGAAATGACTTTTCTA+TGG - chr4.3:3456062-3456081 MS.gene55890:intron 30.0%
! TCATCAAAATGAAAAGCACA+AGG + chr4.3:3455790-3455809 None:intergenic 30.0%
! TTGTTTTCGATCAGATATAG+AGG - chr4.3:3455688-3455707 MS.gene55890:CDS 30.0%
!! GCTGTTTTGCATAATAACAA+AGG + chr4.3:3456162-3456181 None:intergenic 30.0%
ATAGCATATCACAGAAATGG+TGG - chr4.3:3455911-3455930 MS.gene55890:intron 35.0%
CAAAACTATATTCATCCTCC+CGG + chr4.3:3456006-3456025 None:intergenic 35.0%
CTAAAGCAATAAAGGAGATC+AGG - chr4.3:3456201-3456220 MS.gene55890:intron 35.0%
CTCCATAGAAAAGTCATTTC+AGG + chr4.3:3456067-3456086 None:intergenic 35.0%
CTGATCTCCTTTATTGCTTT+AGG + chr4.3:3456203-3456222 None:intergenic 35.0%
GAAAGCTCCTAAAGCAATAA+AGG - chr4.3:3456193-3456212 MS.gene55890:intron 35.0%
! ATTAGTTCCCATAGCCTTTT+GGG + chr4.3:3456234-3456253 None:intergenic 35.0%
!! CACAAAGGTTATTGATGATG+AGG - chr4.3:3456433-3456452 MS.gene55890:CDS 35.0%
!! TCAAGTTGAACAAGGCTATT+TGG - chr4.3:3456270-3456289 MS.gene55890:intron 35.0%
!!! CAAATATTTTGATAACCGGG+AGG - chr4.3:3455988-3456007 MS.gene55890:intron 35.0%
AAGGAAGAGCTCAAAGGATT+GGG - chr4.3:3456410-3456429 MS.gene55890:CDS 40.0%
AAGTTTGCCCAAAAGGCTAT+GGG - chr4.3:3456224-3456243 MS.gene55890:intron 40.0%
AATCCTTTGAGCTCTTCCTT+GGG + chr4.3:3456410-3456429 None:intergenic 40.0%
ATAGAGAGAATTACCAGCCA+TGG + chr4.3:3455615-3455634 None:intergenic 40.0%
TACTGTTGTTGAAATCCCCA+AGG - chr4.3:3456391-3456410 MS.gene55890:CDS 40.0%
! CATTAGTTCCCATAGCCTTT+TGG + chr4.3:3456235-3456254 None:intergenic 40.0%
ATCCTTTGAGCTCTTCCTTG+GGG + chr4.3:3456409-3456428 None:intergenic 45.0%
CAAGGAAGAGCTCAAAGGAT+TGG - chr4.3:3456409-3456428 MS.gene55890:CDS 45.0%
CAATCCTTTGAGCTCTTCCT+TGG + chr4.3:3456411-3456430 None:intergenic 45.0%
GAACGATGATGAGGATGCTA+AGG - chr4.3:3456349-3456368 MS.gene55890:intron 45.0%
GAAGTTTGCCCAAAAGGCTA+TGG - chr4.3:3456223-3456242 MS.gene55890:intron 45.0%
GATCAGGAAGTTTGCCCAAA+AGG - chr4.3:3456217-3456236 MS.gene55890:intron 45.0%
GGGAACTAATGATGTCAGAG+TGG - chr4.3:3456244-3456263 MS.gene55890:intron 45.0%
! AACAAGGCTATTTGGAGCCA+GGG - chr4.3:3456278-3456297 MS.gene55890:intron 45.0%
!! AGTGGACGTCAAGTTGAACA+AGG - chr4.3:3456262-3456281 MS.gene55890:intron 45.0%
AAGTGTCCCTAGGAGGGTTA+GGG - chr4.3:3456307-3456326 MS.gene55890:intron 50.0%
ATCCCCAAGGAAGAGCTCAA+AGG - chr4.3:3456404-3456423 MS.gene55890:CDS 50.0%
CCTCATCATCGTTCCTCTTG+CGG + chr4.3:3456343-3456362 None:intergenic 50.0%
CTCATCATCGTTCCTCTTGC+GGG + chr4.3:3456342-3456361 None:intergenic 50.0%
GACAACCTCTTCCTTCCTAC+CGG + chr4.3:3455557-3455576 None:intergenic 50.0%
GAGAGAATTACCAGCCATGG+AGG + chr4.3:3455612-3455631 None:intergenic 50.0%
GCTCAAAGGATTGGGCACAA+AGG - chr4.3:3456418-3456437 MS.gene55890:CDS 50.0%
! ACAAGGCTATTTGGAGCCAG+GGG - chr4.3:3456279-3456298 MS.gene55890:intron 50.0%
! GAACAAGGCTATTTGGAGCC+AGG - chr4.3:3456277-3456296 MS.gene55890:intron 50.0%
!! GAGGTTGATGGTGTACTCTC+TGG + chr4.3:3455581-3455600 None:intergenic 50.0%
!! GATCAGAAGTGTCCCTAGGA+GGG - chr4.3:3456301-3456320 MS.gene55890:intron 50.0%
AACCTCCACAAACGCCTCCA+TGG - chr4.3:3455595-3455614 MS.gene55890:CDS 55.0%
ATGCGAACCCTAACCCTCCT+AGG + chr4.3:3456317-3456336 None:intergenic 55.0%
CCGCAAGAGGAACGATGATG+AGG - chr4.3:3456340-3456359 MS.gene55890:intron 55.0%
TAGGGACACTTCTGATCCCC+TGG + chr4.3:3456298-3456317 None:intergenic 55.0%
TGCGAACCCTAACCCTCCTA+GGG + chr4.3:3456316-3456335 None:intergenic 55.0%
! GAAGTGTCCCTAGGAGGGTT+AGG - chr4.3:3456306-3456325 MS.gene55890:intron 55.0%
! GAGGCGTTTGTGGAGGTTGA+TGG + chr4.3:3455593-3455612 None:intergenic 55.0%
!! AGGGGATCAGAAGTGTCCCT+AGG - chr4.3:3456297-3456316 MS.gene55890:intron 55.0%
!! GGATCAGAAGTGTCCCTAGG+AGG - chr4.3:3456300-3456319 MS.gene55890:intron 55.0%
TCCACAAACGCCTCCATGGC+TGG - chr4.3:3455599-3455618 MS.gene55890:CDS 60.0%
!! ACCAGCCATGGAGGCGTTTG+TGG + chr4.3:3455603-3455622 None:intergenic 60.0%
!! AGCCATGGAGGCGTTTGTGG+AGG + chr4.3:3455600-3455619 None:intergenic 60.0%
CTCTTCCTTCCTACCGGCGC+CGG + chr4.3:3455551-3455570 None:intergenic 65.0%
GGGTTCGCATTGCCCGCAAG+AGG - chr4.3:3456327-3456346 MS.gene55890:intron 65.0%
! ATGGTGGAGAAGACCGGCGC+CGG - chr4.3:3455535-3455554 MS.gene55890:CDS 65.0%
!! CGGCGCCGGTAGGAAGGAAG+AGG - chr4.3:3455549-3455568 MS.gene55890:CDS 70.0%
!! GAAGACCGGCGCCGGTAGGA+AGG - chr4.3:3455543-3455562 MS.gene55890:CDS 70.0%
!! TGGAGAAGACCGGCGCCGGT+AGG - chr4.3:3455539-3455558 MS.gene55890:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 3455535 3456460 3455535 ID=MS.gene55890
chr4.3 mRNA 3455535 3456460 3455535 ID=MS.gene55890.t1;Parent=MS.gene55890
chr4.3 exon 3456375 3456460 3456375 ID=MS.gene55890.t1.exon1;Parent=MS.gene55890.t1
chr4.3 CDS 3456375 3456460 3456375 ID=cds.MS.gene55890.t1;Parent=MS.gene55890.t1
chr4.3 exon 3455535 3455814 3455535 ID=MS.gene55890.t1.exon2;Parent=MS.gene55890.t1
chr4.3 CDS 3455535 3455814 3455535 ID=cds.MS.gene55890.t1;Parent=MS.gene55890.t1
Gene Sequence

>MS.gene55890

ATGGTGGAGAAGACCGGCGCCGGTAGGAAGGAAGAGGTTGTCACCAGAGAGTACACCATCAACCTCCACAAACGCCTCCATGGCTGGTAATTCTCTCTATTGTTCATTTTTGTTTTTTTCGTTTGTCTAAACCCTATCTAGCAAATGTTTTGTTTGTTTTCGATCAGATATAGAGGAAAATTTTTGTGGATTTTTTATTTATTATATTTTTTAATCTCTTTAGAAGGTTTTTTCAATGGCAGTAAAATAGTCCCTTGTGCTTTTCATTTTGATGATATTGTAATTAAGCTGTTTGAATGTTTAAATGTTCTGTGTCTTTGATATTAATGTTGTCAATTGTGCAATAATGCTGTTAGTTAGCACAATTTTAAGCTAAATAGCATATCACAGAAATGGTGGTTTGCTATGCACTGCGTTTCTAGCAACTCCGTAGATGTTATTTTAACTGATTTACAAATATTTTGATAACCGGGAGGATGAATATAGTTTTGTGAGACAATACTTTGATGTAACAAACATATCCCCTTTACCTGAAATGACTTTTCTATGGAGATTGTTTCGGCCGATATCTCGTTTGTATTATTTGTTTGTTTTAACTTTATGAAATTCTAACAGTCATTTTTTCCTTTGTTATTATGCAAAACAGCACATTTAAGAAGAAAGCTCCTAAAGCAATAAAGGAGATCAGGAAGTTTGCCCAAAAGGCTATGGGAACTAATGATGTCAGAGTGGACGTCAAGTTGAACAAGGCTATTTGGAGCCAGGGGATCAGAAGTGTCCCTAGGAGGGTTAGGGTTCGCATTGCCCGCAAGAGGAACGATGATGAGGATGCTAAGGAAGAGCTCTACTCACTTGTTACTGTTGTTGAAATCCCCAAGGAAGAGCTCAAAGGATTGGGCACAAAGGTTATTGATGATGAGGATTGA

Protein sequence

>MS.gene55890.t1

MVEKTGAGRKEEVVTREYTINLHKRLHGCTFKKKAPKAIKEIRKFAQKAMGTNDVRVDVKLNKAIWSQGIRSVPRRVRVRIARKRNDDEDAKEELYSLVTVVEIPKEELKGLGTKVIDDED