Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064028.t1 | XP_024642002.1 | 100 | 121 | 0 | 0 | 1 | 121 | 1 | 121 | 5.00E-59 | 236.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064028.t1 | Q9M573 | 86.8 | 121 | 16 | 0 | 1 | 121 | 1 | 121 | 1.0e-52 | 207.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064028.t1 | G7KIQ1 | 100.0 | 121 | 0 | 0 | 1 | 121 | 1 | 121 | 3.6e-59 | 236.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050916 | MS.gene064028 | 0.808683 | 2.75E-50 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064028.t1 | MTR_6g091630 | 100.000 | 121 | 0 | 0 | 1 | 121 | 1 | 121 | 7.55e-85 | 243 |
| MS.gene064028.t1 | MTR_8g101980 | 97.521 | 121 | 3 | 0 | 1 | 121 | 1 | 121 | 8.29e-83 | 238 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064028.t1 | AT5G56710 | 84.211 | 114 | 18 | 0 | 8 | 121 | 6 | 119 | 3.45e-67 | 198 |
| MS.gene064028.t1 | AT4G26230 | 83.333 | 114 | 19 | 0 | 8 | 121 | 6 | 119 | 1.30e-66 | 197 |
| MS.gene064028.t1 | AT2G19740 | 81.667 | 120 | 21 | 1 | 2 | 121 | 1 | 119 | 4.59e-66 | 196 |
| MS.gene064028.t1 | AT5G56710 | 58.772 | 114 | 13 | 1 | 8 | 121 | 6 | 85 | 6.30e-38 | 123 |
Find 36 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGATCTCCTTGATAGCTTT+TGG | 0.199036 | 6.1:+79287088 | None:intergenic |
| ATTCCTAAAGAAGAGTTAAA+AGG | 0.277484 | 6.1:-79286884 | MS.gene064028:CDS |
| TAAAGAAGAGTTAAAAGGTT+TGG | 0.305277 | 6.1:-79286879 | MS.gene064028:CDS |
| TCAAGCTGAACAAGGCTATC+TGG | 0.323250 | 6.1:-79287018 | MS.gene064028:CDS |
| AAAGAAGAGTTAAAAGGTTT+GGG | 0.335880 | 6.1:-79286878 | MS.gene064028:CDS |
| GAAGTGTACCAAGGAGGATC+AGG | 0.398880 | 6.1:-79286982 | MS.gene064028:CDS |
| TTGGTACACTTCTGATTCCC+TGG | 0.413987 | 6.1:+79286993 | None:intergenic |
| AAGATTGAAATTACCATCCA+TGG | 0.468912 | 6.1:+79289027 | None:intergenic |
| GAAGGCACCAAAAGCTATCA+AGG | 0.472855 | 6.1:-79287095 | MS.gene064028:CDS |
| GAACAAGGCTATCTGGAGCC+AGG | 0.480113 | 6.1:-79287011 | MS.gene064028:CDS |
| ATGGTGGAGAAGGGTGCAGC+AGG | 0.507040 | 6.1:-79289104 | MS.gene064028:CDS |
| ATGCGTACCCTGATCCTCCT+TGG | 0.524657 | 6.1:+79286974 | None:intergenic |
| GAGACGCTTGTGGAGATTGA+TGG | 0.526766 | 6.1:+79289049 | None:intergenic |
| AAGGGAGTATAACTCTTCCT+TGG | 0.536523 | 6.1:+79286922 | None:intergenic |
| GAAGCTGAAAATGGTGGAGA+AGG | 0.536786 | 6.1:-79289114 | None:intergenic |
| TCTAACATCGTTAGTGCCCA+TGG | 0.538776 | 6.1:+79287048 | None:intergenic |
| ATCTTCATCATCAATGACCT+TGG | 0.551546 | 6.1:+79286853 | None:intergenic |
| AAACAGCACCTTCAAGAAGA+AGG | 0.555549 | 6.1:-79287113 | MS.gene064028:intron |
| AATCTCCACAAGCGTCTCCA+TGG | 0.563199 | 6.1:-79289044 | MS.gene064028:CDS |
| TCCACAAGCGTCTCCATGGA+TGG | 0.567901 | 6.1:-79289040 | MS.gene064028:intron |
| TGCAGCAGGTAGAAAAGAAG+AGG | 0.581022 | 6.1:-79289090 | MS.gene064028:CDS |
| AAAAGAAGAGGTGGTTACAC+GGG | 0.584738 | 6.1:-79289078 | MS.gene064028:CDS |
| GAAAAGAAGAGGTGGTTACA+CGG | 0.586191 | 6.1:-79289079 | MS.gene064028:CDS |
| ACCATCCATGGAGACGCTTG+TGG | 0.589153 | 6.1:+79289039 | None:intergenic |
| GAATTTCAACGACAGTGACA+AGG | 0.604839 | 6.1:+79286903 | None:intergenic |
| AAGCTGAAAATGGTGGAGAA+GGG | 0.607163 | 6.1:-79289113 | None:intergenic |
| GAATGACGACGAAGATGCCA+AGG | 0.608749 | 6.1:-79286939 | MS.gene064028:CDS |
| AACAAGGCTATCTGGAGCCA+GGG | 0.614836 | 6.1:-79287010 | MS.gene064028:CDS |
| AAGTGTACCAAGGAGGATCA+GGG | 0.622533 | 6.1:-79286981 | MS.gene064028:CDS |
| AGTTGATGTCAAGCTGAACA+AGG | 0.626602 | 6.1:-79287026 | MS.gene064028:CDS |
| GTTAAAAGGTTTGGGCACCA+AGG | 0.626806 | 6.1:-79286870 | MS.gene064028:CDS |
| AGCAGGTAGAAAAGAAGAGG+TGG | 0.632837 | 6.1:-79289087 | MS.gene064028:CDS |
| AATTTCAACGACAGTGACAA+GGG | 0.644182 | 6.1:+79286904 | None:intergenic |
| GGGTACGCATTGCTCGCAAG+AGG | 0.652748 | 6.1:-79286961 | MS.gene064028:CDS |
| AGGGAATCAGAAGTGTACCA+AGG | 0.682823 | 6.1:-79286991 | MS.gene064028:CDS |
| GAATCAGAAGTGTACCAAGG+AGG | 0.739011 | 6.1:-79286988 | MS.gene064028:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTCATGATTTGAAAAAT+CGG | - | chr6.1:79288633-79288652 | MS.gene064028:intron | 15.0% |
| !! | AGCACTTATAATATTATAAG+CGG | + | chr6.1:79288016-79288035 | None:intergenic | 20.0% |
| !! | ATTAAACTGTTAATCAAAAC+AGG | + | chr6.1:79287990-79288009 | None:intergenic | 20.0% |
| !! | TTAAACTGTTAATCAAAACA+GGG | + | chr6.1:79287989-79288008 | None:intergenic | 20.0% |
| !! | TTATGACGAGATAAAAATAA+AGG | - | chr6.1:79287190-79287209 | MS.gene064028:intron | 20.0% |
| !! | TTATGTAACACAACAATTAT+GGG | + | chr6.1:79287442-79287461 | None:intergenic | 20.0% |
| !! | TTGATATTCATATTGTGAAT+TGG | + | chr6.1:79288663-79288682 | None:intergenic | 20.0% |
| !!! | AAGCTATTTTTATAAGCTAA+GGG | - | chr6.1:79287235-79287254 | MS.gene064028:intron | 20.0% |
| !!! | CTGTGTTTATTAGATAATTT+TGG | - | chr6.1:79287334-79287353 | MS.gene064028:intron | 20.0% |
| !!! | TAAGCTATTTTTATAAGCTA+AGG | - | chr6.1:79287234-79287253 | MS.gene064028:intron | 20.0% |
| ! | AAAAATAAGCCAATCTAAAC+AGG | + | chr6.1:79287261-79287280 | None:intergenic | 25.0% |
| ! | AAAGAAGAGTTAAAAGGTTT+GGG | - | chr6.1:79289076-79289095 | MS.gene064028:CDS | 25.0% |
| ! | AAATATGTATGCTGTTCATT+TGG | - | chr6.1:79288468-79288487 | MS.gene064028:intron | 25.0% |
| ! | AATGAACAGCATACATATTT+AGG | + | chr6.1:79288468-79288487 | None:intergenic | 25.0% |
| ! | ACTAAGTTATTTATCCATAC+AGG | + | chr6.1:79287112-79287131 | None:intergenic | 25.0% |
| ! | ATGAACAGCATACATATTTA+GGG | + | chr6.1:79288467-79288486 | None:intergenic | 25.0% |
| ! | ATGAATTCAAAAACAGCTTA+TGG | + | chr6.1:79288358-79288377 | None:intergenic | 25.0% |
| ! | ATTCCTAAAGAAGAGTTAAA+AGG | - | chr6.1:79289070-79289089 | MS.gene064028:CDS | 25.0% |
| ! | CTAAGTTATTTATCCATACA+GGG | + | chr6.1:79287111-79287130 | None:intergenic | 25.0% |
| ! | CTTATGTAACACAACAATTA+TGG | + | chr6.1:79287443-79287462 | None:intergenic | 25.0% |
| ! | GTGAGTAAATTAATAGTTTG+TGG | - | chr6.1:79288490-79288509 | MS.gene064028:intron | 25.0% |
| ! | TAAAGAAGAGTTAAAAGGTT+TGG | - | chr6.1:79289075-79289094 | MS.gene064028:CDS | 25.0% |
| ! | TATGAAAATAAGCTGAATAC+AGG | + | chr6.1:79287847-79287866 | None:intergenic | 25.0% |
| ! | TATGTAACACAACAATTATG+GGG | + | chr6.1:79287441-79287460 | None:intergenic | 25.0% |
| ! | TGCTAATTTCTGTTTATGTT+TGG | - | chr6.1:79288799-79288818 | MS.gene064028:intron | 25.0% |
| ! | TTTCATGATCTAACTACATT+AGG | - | chr6.1:79287963-79287982 | MS.gene064028:intron | 25.0% |
| !! | AGTGATGAAATTGATGATTA+TGG | + | chr6.1:79287932-79287951 | None:intergenic | 25.0% |
| !! | ATACATATTTAGGGTTTGTT+TGG | + | chr6.1:79288458-79288477 | None:intergenic | 25.0% |
| !! | CTCAAATAAGCTATTTCAAA+AGG | - | chr6.1:79288201-79288220 | MS.gene064028:intron | 25.0% |
| !! | TCAATTTTGTAGATCTACTA+GGG | - | chr6.1:79287716-79287735 | MS.gene064028:intron | 25.0% |
| !! | TTCAATTTTGTAGATCTACT+AGG | - | chr6.1:79287715-79287734 | MS.gene064028:intron | 25.0% |
| !!! | AAACCTTTTAACTCTTCTTT+AGG | + | chr6.1:79289076-79289095 | None:intergenic | 25.0% |
| !!! | GCTGTTTTAAAAAAACATCA+TGG | + | chr6.1:79287876-79287895 | None:intergenic | 25.0% |
| !!! | TAATATGAATTGAGTTGAGT+TGG | - | chr6.1:79288516-79288535 | MS.gene064028:intron | 25.0% |
| !!! | TCCTGTAATATTGTTTTAGT+TGG | - | chr6.1:79287689-79287708 | MS.gene064028:intron | 25.0% |
| AAGATTGAAATTACCATCCA+TGG | + | chr6.1:79286930-79286949 | None:intergenic | 30.0% | |
| ATGTAACACAACAATTATGG+GGG | + | chr6.1:79287440-79287459 | None:intergenic | 30.0% | |
| ATTCTCTAATGGCATAACAT+TGG | + | chr6.1:79288181-79288200 | None:intergenic | 30.0% | |
| GCCAACTAAAACAATATTAC+AGG | + | chr6.1:79287693-79287712 | None:intergenic | 30.0% | |
| GCTATGTGTAATTGCATAAT+TGG | - | chr6.1:79288737-79288756 | MS.gene064028:intron | 30.0% | |
| TAGGATCTGTTTGGATAAAT+AGG | - | chr6.1:79287482-79287501 | MS.gene064028:intron | 30.0% | |
| TGTCATCAACTGTTTCAATA+AGG | + | chr6.1:79287815-79287834 | None:intergenic | 30.0% | |
| ! | AAAATTTGCTCAAAAAGCCA+TGG | - | chr6.1:79288889-79288908 | MS.gene064028:intron | 30.0% |
| ! | AAATTTGCTCAAAAAGCCAT+GGG | - | chr6.1:79288890-79288909 | MS.gene064028:intron | 30.0% |
| ! | GCTTATTTGAGATTCTCTAA+TGG | + | chr6.1:79288192-79288211 | None:intergenic | 30.0% |
| ! | TATTTAGGGTTTGTTTGGAT+AGG | + | chr6.1:79288453-79288472 | None:intergenic | 30.0% |
| ! | TCAGTTTTCTGTGTTTACAT+TGG | - | chr6.1:79287635-79287654 | MS.gene064028:intron | 30.0% |
| !! | CGTTTCAAATTTGTTGATCT+AGG | - | chr6.1:79287463-79287482 | MS.gene064028:intron | 30.0% |
| !!! | TGTTTTTTATAAGCGACTCT+TGG | - | chr6.1:79288081-79288100 | MS.gene064028:intron | 30.0% |
| AATTTCAACGACAGTGACAA+GGG | + | chr6.1:79289053-79289072 | None:intergenic | 35.0% | |
| ATCTTCATCATCAATGACCT+TGG | + | chr6.1:79289104-79289123 | None:intergenic | 35.0% | |
| TGTCATTGTTCATAAGTTGC+TGG | + | chr6.1:79288399-79288418 | None:intergenic | 35.0% | |
| ! | TTGTTGATCTAGGATCTGTT+TGG | - | chr6.1:79287473-79287492 | MS.gene064028:intron | 35.0% |
| AAAAGAAGAGGTGGTTACAC+GGG | - | chr6.1:79286876-79286895 | MS.gene064028:CDS | 40.0% | |
| AAACAGCACCTTCAAGAAGA+AGG | - | chr6.1:79288841-79288860 | MS.gene064028:intron | 40.0% | |
| AACACAACAATTATGGGGGA+AGG | + | chr6.1:79287436-79287455 | None:intergenic | 40.0% | |
| CTATAGATCTAGACCCTGTA+TGG | - | chr6.1:79287095-79287114 | MS.gene064028:CDS | 40.0% | |
| CTATTTGCATAAGCTCCTGT+TGG | - | chr6.1:79287776-79287795 | MS.gene064028:intron | 40.0% | |
| GAAAAGAAGAGGTGGTTACA+CGG | - | chr6.1:79286875-79286894 | MS.gene064028:CDS | 40.0% | |
| GAATTTCAACGACAGTGACA+AGG | + | chr6.1:79289054-79289073 | None:intergenic | 40.0% | |
| ! | AAGGGAGTATAACTCTTCCT+TGG | + | chr6.1:79289035-79289054 | None:intergenic | 40.0% |
| ! | AGTTGATGTCAAGCTGAACA+AGG | - | chr6.1:79288928-79288947 | MS.gene064028:intron | 40.0% |
| ! | ATCTACTAGGGTGTTAGGAT+CGG | - | chr6.1:79287728-79287747 | MS.gene064028:intron | 40.0% |
| ! | TGTAGATCTACTAGGGTGTT+AGG | - | chr6.1:79287723-79287742 | MS.gene064028:intron | 40.0% |
| ! | TTCATAAGTTGCTGGCTGTT+TGG | + | chr6.1:79288391-79288410 | None:intergenic | 40.0% |
| !! | CTGATCTCCTTGATAGCTTT+TGG | + | chr6.1:79288869-79288888 | None:intergenic | 40.0% |
| !!! | TTTTGGTGCCTTCTTCTTGA+AGG | + | chr6.1:79288852-79288871 | None:intergenic | 40.0% |
| AAGCTCCTGTTGGACTGTTT+GGG | - | chr6.1:79287786-79287805 | MS.gene064028:intron | 45.0% | |
| AAGTGTACCAAGGAGGATCA+GGG | - | chr6.1:79288973-79288992 | MS.gene064028:intron | 45.0% | |
| AGCAGGTAGAAAAGAAGAGG+TGG | - | chr6.1:79286867-79286886 | MS.gene064028:CDS | 45.0% | |
| AGCTAAGGGCCTGTTTAGAT+TGG | - | chr6.1:79287249-79287268 | MS.gene064028:intron | 45.0% | |
| GTTAAAAGGTTTGGGCACCA+AGG | - | chr6.1:79289084-79289103 | MS.gene064028:CDS | 45.0% | |
| TAAGCTCCTGTTGGACTGTT+TGG | - | chr6.1:79287785-79287804 | MS.gene064028:intron | 45.0% | |
| TCAAGCTGAACAAGGCTATC+TGG | - | chr6.1:79288936-79288955 | MS.gene064028:intron | 45.0% | |
| TCTAACATCGTTAGTGCCCA+TGG | + | chr6.1:79288909-79288928 | None:intergenic | 45.0% | |
| TGCAGCAGGTAGAAAAGAAG+AGG | - | chr6.1:79286864-79286883 | MS.gene064028:CDS | 45.0% | |
| TTGGTACACTTCTGATTCCC+TGG | + | chr6.1:79288964-79288983 | None:intergenic | 45.0% | |
| ! | AGGGAATCAGAAGTGTACCA+AGG | - | chr6.1:79288963-79288982 | MS.gene064028:intron | 45.0% |
| ! | GAATCAGAAGTGTACCAAGG+AGG | - | chr6.1:79288966-79288985 | MS.gene064028:intron | 45.0% |
| ! | TCGCACTGTTTTGTGTGTTG+TGG | - | chr6.1:79288551-79288570 | MS.gene064028:intron | 45.0% |
| !! | GAAGGCACCAAAAGCTATCA+AGG | - | chr6.1:79288859-79288878 | MS.gene064028:intron | 45.0% |
| !! | TTAATTAAAATTAAAATCAT+GGG | + | chr6.1:79287065-79287084 | None:intergenic | 5.0% |
| !! | TTTAATTAAAATTAAAATCA+TGG | + | chr6.1:79287066-79287085 | None:intergenic | 5.0% |
| AATCTCCACAAGCGTCTCCA+TGG | - | chr6.1:79286910-79286929 | MS.gene064028:CDS | 50.0% | |
| AGCGACTCTTGGAGAGCTTA+TGG | - | chr6.1:79288092-79288111 | MS.gene064028:intron | 50.0% | |
| GAAGTGTACCAAGGAGGATC+AGG | - | chr6.1:79288972-79288991 | MS.gene064028:intron | 50.0% | |
| GAATGACGACGAAGATGCCA+AGG | - | chr6.1:79289015-79289034 | MS.gene064028:intron | 50.0% | |
| GAGACGCTTGTGGAGATTGA+TGG | + | chr6.1:79286908-79286927 | None:intergenic | 50.0% | |
| ! | AACAAGGCTATCTGGAGCCA+GGG | - | chr6.1:79288944-79288963 | MS.gene064028:intron | 50.0% |
| ! | CTAGGGTGTTAGGATCGGTT+TGG | - | chr6.1:79287733-79287752 | MS.gene064028:intron | 50.0% |
| ACCATCCATGGAGACGCTTG+TGG | + | chr6.1:79286918-79286937 | None:intergenic | 55.0% | |
| ATGCGTACCCTGATCCTCCT+TGG | + | chr6.1:79288983-79289002 | None:intergenic | 55.0% | |
| GGTCTCCCAAACAGTCCAAC+AGG | + | chr6.1:79287794-79287813 | None:intergenic | 55.0% | |
| TCCACAAGCGTCTCCATGGA+TGG | - | chr6.1:79286914-79286933 | MS.gene064028:CDS | 55.0% | |
| ! | GAACAAGGCTATCTGGAGCC+AGG | - | chr6.1:79288943-79288962 | MS.gene064028:intron | 55.0% |
| GGGTACGCATTGCTCGCAAG+AGG | - | chr6.1:79288993-79289012 | MS.gene064028:intron | 60.0% | |
| ! | ATGGTGGAGAAGGGTGCAGC+AGG | - | chr6.1:79286850-79286869 | MS.gene064028:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 79286850 | 79289126 | 79286850 | ID=MS.gene064028 |
| chr6.1 | mRNA | 79286850 | 79289126 | 79286850 | ID=MS.gene064028.t1;Parent=MS.gene064028 |
| chr6.1 | exon | 79289041 | 79289126 | 79289041 | ID=MS.gene064028.t1.exon1;Parent=MS.gene064028.t1 |
| chr6.1 | CDS | 79289041 | 79289126 | 79289041 | ID=cds.MS.gene064028.t1;Parent=MS.gene064028.t1 |
| chr6.1 | exon | 79286850 | 79287129 | 79286850 | ID=MS.gene064028.t1.exon2;Parent=MS.gene064028.t1 |
| chr6.1 | CDS | 79286850 | 79287129 | 79286850 | ID=cds.MS.gene064028.t1;Parent=MS.gene064028.t1 |
| Gene Sequence |
| Protein sequence |