AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031484

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031484.t1 MTR_4g081850 92.362 563 33 1 1 553 1 563 0.0 1040
MS.gene031484.t1 MTR_2g079510 88.414 561 57 2 1 553 1 561 0.0 1016
MS.gene031484.t1 MTR_1g048030 33.420 383 229 5 189 546 125 506 2.23e-58 205
MS.gene031484.t1 MTR_4g050490 28.721 383 190 11 185 492 150 524 8.40e-34 135
MS.gene031484.t1 MTR_5g010400 65.278 72 25 0 7 78 14 85 3.11e-23 94.4
MS.gene031484.t1 MTR_7g094890 25.843 267 166 8 198 445 110 363 2.14e-15 78.6
MS.gene031484.t1 MTR_2g079540 70.000 50 13 1 7 54 15 64 1.29e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031484.t1 AT1G52980 72.080 548 137 3 4 535 3 550 0.0 783
MS.gene031484.t1 AT3G07050 35.714 308 174 2 169 452 100 407 4.70e-61 212
MS.gene031484.t1 AT2G27200 30.031 323 169 6 185 452 149 469 1.47e-36 143
MS.gene031484.t1 AT1G08410 27.614 373 201 8 185 490 149 519 1.54e-35 140
MS.gene031484.t1 AT4G02790 26.115 314 198 9 155 447 64 364 1.46e-17 85.1

Find 128 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATATTTGCAATACCTTTAA+TGG 0.138476 4.2:+27659431 None:intergenic
CTTTCAAATGCTTCTCTAAA+TGG 0.150218 4.2:+27660699 None:intergenic
GGTCAGGTTCACCGCCTTTA+AGG 0.208215 4.2:+27659613 None:intergenic
GCAAGCATTAATAAGTCTTT+TGG 0.224309 4.2:-27660557 MS.gene031484:CDS
CTTACAGGTTGCTCCAATTC+CGG 0.231915 4.2:-27659904 MS.gene031484:intron
CAGGCATCTGCTGCCATTAA+AGG 0.234669 4.2:-27659444 MS.gene031484:CDS
TCATCTGGCAAACTCCTTAA+AGG 0.256903 4.2:-27659627 MS.gene031484:CDS
ATATAATGAGAATGAGTTAA+AGG 0.292360 4.2:-27659364 MS.gene031484:CDS
GGCTGAGCGTGACTGGATTT+TGG 0.293828 4.2:+27661291 None:intergenic
AAGAAAACGTCCAAGTCTTT+TGG 0.296672 4.2:-27660942 MS.gene031484:CDS
AACCCGATCGCCAATGGTTC+GGG 0.298498 4.2:-27661159 MS.gene031484:CDS
TATGAGGAGCCTAATGTTAA+TGG 0.328014 4.2:-27659498 MS.gene031484:CDS
ATTAGAGTGCTTTGGTTTAC+CGG 0.328797 4.2:+27661336 None:intergenic
ACCGTAAGGGCCGTGTCTTA+TGG 0.341749 4.2:-27661222 MS.gene031484:CDS
CTTACGGTTGCGGACGGGTT+TGG 0.345951 4.2:+27661237 None:intergenic
ATGATTACCAGAAGCAATCT+AGG 0.346051 4.2:-27661018 MS.gene031484:CDS
CCGTAAGGGCCGTGTCTTAT+GGG 0.347948 4.2:-27661221 MS.gene031484:CDS
TTACAGGTTGCTCCAATTCC+GGG 0.351375 4.2:-27659903 MS.gene031484:intron
CAGTGGCGGCTGAGCGTGAC+TGG 0.355736 4.2:+27661284 None:intergenic
ACAGATGACTTCCCAACATT+TGG 0.356765 4.2:+27660449 None:intergenic
AAACCCGATCGCCAATGGTT+CGG 0.366201 4.2:-27661160 MS.gene031484:CDS
GAACAAGTGTGATCTAATTC+CGG 0.392284 4.2:-27660642 MS.gene031484:CDS
TATAATGAGAATGAGTTAAA+GGG 0.392821 4.2:-27659363 MS.gene031484:CDS
GGTGAACCCTAGATTGCTTC+TGG 0.393253 4.2:+27661011 None:intergenic
TGGGAGCAGTATCAACTATC+AGG 0.394601 4.2:-27661202 MS.gene031484:CDS
GGGCAACGAAAGGATGGCTT+AGG 0.399832 4.2:-27660616 MS.gene031484:CDS
TTCTTCAGATGTTGTTGTGC+AGG 0.401568 4.2:-27660759 MS.gene031484:CDS
AACATTAGGCTCCTCATACT+TGG 0.402135 4.2:+27659503 None:intergenic
GTTTCAGAACCTCGCCTATA+TGG 0.402474 4.2:+27659733 None:intergenic
TTCCCGAACCATTGGCGATC+GGG 0.409646 4.2:+27661157 None:intergenic
GAAACTCGTTATTCATTTGT+TGG 0.421464 4.2:+27659224 None:intergenic
CCCATAAGACACGGCCCTTA+CGG 0.423389 4.2:+27661221 None:intergenic
CTTCAGCTTTGCAAATCATC+TGG 0.430614 4.2:-27659642 MS.gene031484:CDS
AAACTCGTTATTCATTTGTT+GGG 0.434048 4.2:+27659225 None:intergenic
ACATCAATGCCATTAACATT+AGG 0.437807 4.2:+27659489 None:intergenic
AAAGATGCTGCTGACCATAT+AGG 0.439860 4.2:-27659747 MS.gene031484:CDS
TGGAAAGGGTTCACTTCTAT+CGG 0.440433 4.2:-27660537 MS.gene031484:CDS
TGGCAGCAGATGCCTGGTTA+TGG 0.442559 4.2:+27659451 None:intergenic
TTGGCGTCATTAGAGTGCTT+TGG 0.444184 4.2:+27661328 None:intergenic
AGGGTCAAAGCAAACGCATA+TGG 0.452368 4.2:-27660805 MS.gene031484:CDS
TTTGTTGGATATCCAAATGT+TGG 0.456100 4.2:-27660461 MS.gene031484:CDS
GTGTGATCTAATTCCGGCTT+GGG 0.461353 4.2:-27660636 MS.gene031484:CDS
GCGACATACCATGTTTGAAA+AGG 0.465171 4.2:-27660825 MS.gene031484:CDS
TTTGGAGTCATTGCTGCGGT+TGG 0.465405 4.2:+27661309 None:intergenic
TTATATAAATCACCTTGAAC+TGG 0.466516 4.2:+27659381 None:intergenic
TTGTTGGATATCCAAATGTT+GGG 0.472784 4.2:-27660460 MS.gene031484:CDS
CAACAAAGTGTACCAGTTCA+AGG 0.472963 4.2:-27659393 MS.gene031484:CDS
GGGTCAAAGCAAACGCATAT+GGG 0.476137 4.2:-27660804 MS.gene031484:CDS
CTTTAATGGCAGCAGATGCC+TGG 0.487148 4.2:+27659445 None:intergenic
TCATCTTTGCAGCAGTCATA+AGG 0.488979 4.2:+27659592 None:intergenic
GGAAGACCTGTCCAAGTATG+AGG 0.491469 4.2:-27659514 MS.gene031484:CDS
AAGACTTATTAATGCTTGCA+TGG 0.501286 4.2:+27660561 None:intergenic
TAATTATAATGTTATCATCA+AGG 0.504072 4.2:-27661068 MS.gene031484:CDS
GAAAGTAATGAAGCTGATGA+TGG 0.504709 4.2:-27660863 MS.gene031484:CDS
CGGCCCTTACGGTTGCGGAC+GGG 0.508314 4.2:+27661232 None:intergenic
TTGTTGTGGTAGACAACTCC+TGG 0.509529 4.2:+27659822 None:intergenic
GTTTGATTCTCGTGGATGGA+AGG 0.510865 4.2:+27661179 None:intergenic
TAATCAGAAGCCAAAAGACT+TGG 0.511610 4.2:+27660932 None:intergenic
GAGAGAAGCGTGAATGTCTC+CGG 0.514912 4.2:-27661355 MS.gene031484:CDS
CAAACCCGTCCGCAACCGTA+AGG 0.516484 4.2:-27661236 MS.gene031484:CDS
GGTTCTGAAACGTGTGAAGA+AGG 0.518306 4.2:-27659721 MS.gene031484:CDS
CGTGAGGATTCCCGAACCAT+TGG 0.518846 4.2:+27661149 None:intergenic
CGACATACCATGTTTGAAAA+GGG 0.520906 4.2:-27660824 MS.gene031484:CDS
AGACACGGCCCTTACGGTTG+CGG 0.521696 4.2:+27661227 None:intergenic
TCGGGTTTGATTCTCGTGGA+TGG 0.524475 4.2:+27661175 None:intergenic
TTTGCAGCAGTCATAAGGTC+AGG 0.530012 4.2:+27659597 None:intergenic
AATTATAATGTTATCATCAA+GGG 0.531967 4.2:-27661067 MS.gene031484:CDS
AGCCTATAAAATAAAAGAGT+GGG 0.532348 4.2:-27659685 MS.gene031484:CDS
GAATCAAACCCGATCGCCAA+TGG 0.532452 4.2:-27661165 MS.gene031484:CDS
CATCCTTTCGTTGCCCAAGC+CGG 0.533687 4.2:+27660623 None:intergenic
CGGCTTGGGCAACGAAAGGA+TGG 0.538655 4.2:-27660622 MS.gene031484:CDS
CAAAGATGATACTTCATGAC+TGG 0.544123 4.2:-27659578 MS.gene031484:CDS
GGCAACGAAAGGATGGCTTA+GGG 0.546997 4.2:-27660615 MS.gene031484:CDS
TGCTGACAAACTTCTGAACT+CGG 0.547967 4.2:-27659334 MS.gene031484:CDS
GAGCCTATAAAATAAAAGAG+TGG 0.550840 4.2:-27659686 MS.gene031484:CDS
ATTCCCGAACCATTGGCGAT+CGG 0.551780 4.2:+27661156 None:intergenic
ACGGCCCTTACGGTTGCGGA+CGG 0.551921 4.2:+27661231 None:intergenic
TGAAACAGATGTTGTGCTGA+AGG 0.552137 4.2:-27659793 MS.gene031484:CDS
TGATACTTCATGACTGGCAG+AGG 0.552770 4.2:-27659572 MS.gene031484:CDS
GAACAGCTTACTTCTGCAGC+TGG 0.555564 4.2:-27659249 MS.gene031484:CDS
TCAGATGGAACCTCGGTGGA+TGG 0.556459 4.2:+27659270 None:intergenic
TTGTGCAGGTTATAGATGCA+AGG 0.560683 4.2:-27660745 MS.gene031484:CDS
CATATGGGGTGAACTCTACA+AGG 0.561859 4.2:-27660789 MS.gene031484:CDS
ATGATTTGCAAAGCTGAAGT+AGG 0.563324 4.2:+27659646 None:intergenic
TGGTAACATCTTGTACCTTG+TGG 0.564112 4.2:+27660719 None:intergenic
AGTGTGATCTAATTCCGGCT+TGG 0.566088 4.2:-27660637 MS.gene031484:CDS
TAGGCTCCTCATACTTGGAC+AGG 0.566773 4.2:+27659508 None:intergenic
TTGGACAGGTCTTCCAGCCA+TGG 0.567698 4.2:+27659522 None:intergenic
AGATAGCGACACATTTGAGC+AGG 0.568986 4.2:-27659295 MS.gene031484:CDS
TCTTTCTGATTCACAACGTG+AGG 0.569890 4.2:+27661133 None:intergenic
TACAGGTTGCTCCAATTCCG+GGG 0.570126 4.2:-27659902 MS.gene031484:intron
GCGTACTAAGAATGTTTGCA+AGG 0.578208 4.2:-27660414 MS.gene031484:intron
GCAGAAGTAAGCTGTTCAGA+TGG 0.582024 4.2:+27659255 None:intergenic
CTTGCATGGAATGCTAGAGT+TGG 0.588926 4.2:+27660575 None:intergenic
TCTTTAGCTAAGAAAGCTGA+TGG 0.590099 4.2:-27660905 MS.gene031484:CDS
GCGATCGGGTTTGATTCTCG+TGG 0.591809 4.2:+27661171 None:intergenic
AGGTCTTCCAGCCATGGAGG+AGG 0.591963 4.2:+27659528 None:intergenic
TGGTATTGTACGTCTTGAGA+CGG 0.597906 4.2:+27661257 None:intergenic
TGATTACCAGAAGCAATCTA+GGG 0.600894 4.2:-27661017 MS.gene031484:CDS
CTTGAGACGGCGAACAGTGG+CGG 0.602056 4.2:+27661270 None:intergenic
AAACCCGTCCGCAACCGTAA+GGG 0.603072 4.2:-27661235 MS.gene031484:CDS
ATTCCGGCTTGGGCAACGAA+AGG 0.603902 4.2:-27660626 MS.gene031484:CDS
GATAAACAAGCAATATCTGT+CGG 0.606616 4.2:-27660485 MS.gene031484:CDS
GTAATCATTGAGAAGCGACA+TGG 0.608065 4.2:+27661033 None:intergenic
TGTGCAGGTTATAGATGCAA+GGG 0.613175 4.2:-27660744 MS.gene031484:CDS
GATACTTCATGACTGGCAGA+GGG 0.617838 4.2:-27659571 MS.gene031484:CDS
ATTATAATGTTATCATCAAG+GGG 0.619969 4.2:-27661066 MS.gene031484:CDS
AGTATATAACCCTTACAAAG+AGG 0.626422 4.2:-27659863 MS.gene031484:CDS
GAAACAGATGTTGTGCTGAA+GGG 0.627267 4.2:-27659792 MS.gene031484:CDS
AGATGTTGTGCTGAAGGGCG+TGG 0.627685 4.2:-27659787 MS.gene031484:CDS
CTGTTTCAGAGTCATTGTTG+TGG 0.635053 4.2:+27659808 None:intergenic
GGTCAAAGCAAACGCATATG+GGG 0.635960 4.2:-27660803 MS.gene031484:CDS
GACAGGTCTTCCAGCCATGG+AGG 0.641023 4.2:+27659525 None:intergenic
TGAGGCAGTTGACCATAACC+AGG 0.646672 4.2:-27659463 MS.gene031484:CDS
TGCTGCTGACCATATAGGCG+AGG 0.647460 4.2:-27659742 MS.gene031484:CDS
AAATTGCAAGCACAAACACA+TGG 0.648597 4.2:-27660675 MS.gene031484:CDS
CGTCTTGAGACGGCGAACAG+TGG 0.652459 4.2:+27661267 None:intergenic
ACAGGTTGCTCCAATTCCGG+GGG 0.652730 4.2:-27659901 MS.gene031484:intron
TAATGGCATTGATGTTGATG+AGG 0.665181 4.2:-27659481 MS.gene031484:CDS
TAATCATTGAGAAGCGACAT+GGG 0.665391 4.2:+27661034 None:intergenic
CTGTTCAGATGGAACCTCGG+TGG 0.679880 4.2:+27659266 None:intergenic
AAGCTGTTCAGATGGAACCT+CGG 0.685311 4.2:+27659263 None:intergenic
TGACAAACTTCTGAACTCGG+AGG 0.694828 4.2:-27659331 MS.gene031484:CDS
TCTGGCAAACTCCTTAAAGG+CGG 0.702803 4.2:-27659624 MS.gene031484:CDS
GATACTGCTCCCATAAGACA+CGG 0.708733 4.2:+27661212 None:intergenic
ATACTTCATGACTGGCAGAG+GGG 0.723483 4.2:-27659570 MS.gene031484:CDS
TGAGCAGGATCCATCCACCG+AGG 0.767844 4.2:-27659280 MS.gene031484:CDS
ATAGATGCAAGGGATCCACA+AGG 0.773503 4.2:-27660734 MS.gene031484:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTATTAATTATTCTTAC+AGG - chr4.2:27660687-27660706 MS.gene031484:CDS 10.0%
!! TTAATAATATAATTGCTTTA+TGG + chr4.2:27660677-27660696 None:intergenic 10.0%
!! AATTATAATGTTATCATCAA+GGG - chr4.2:27659539-27659558 MS.gene031484:CDS 15.0%
!! TAATTATAATGTTATCATCA+AGG - chr4.2:27659538-27659557 MS.gene031484:CDS 15.0%
!! AATATTTGCAATACCTTTAA+TGG + chr4.2:27661178-27661197 None:intergenic 20.0%
!! ATATAATGAGAATGAGTTAA+AGG - chr4.2:27661242-27661261 MS.gene031484:CDS 20.0%
!! ATTATAATGTTATCATCAAG+GGG - chr4.2:27659540-27659559 MS.gene031484:CDS 20.0%
!! TATAATGAGAATGAGTTAAA+GGG - chr4.2:27661243-27661262 MS.gene031484:CDS 20.0%
!! TTACATGTAATTATAGAAAG+AGG - chr4.2:27660357-27660376 MS.gene031484:intron 20.0%
!!! AAACATTCGCTATAATTTTT+TGG + chr4.2:27660397-27660416 None:intergenic 20.0%
!!! ATTAATAAGTCTTTTGGAAA+GGG - chr4.2:27660055-27660074 MS.gene031484:intron 20.0%
! AATCAAAATTCTCACTTAAG+CGG - chr4.2:27660442-27660461 MS.gene031484:CDS 25.0%
! AATTACTAACTAATGCACAA+AGG + chr4.2:27660329-27660348 None:intergenic 25.0%
! AGCCTATAAAATAAAAGAGT+GGG - chr4.2:27660921-27660940 MS.gene031484:CDS 25.0%
! ATTATTCATGTGAATGTGAA+AGG + chr4.2:27660633-27660652 None:intergenic 25.0%
! TTATATAAATCACCTTGAAC+TGG + chr4.2:27661228-27661247 None:intergenic 25.0%
!!! AACCCACTCTTTTATTTTAT+AGG + chr4.2:27660926-27660945 None:intergenic 25.0%
!!! AGAGTTTTTTAGAGAAGAAA+TGG - chr4.2:27659499-27659518 MS.gene031484:CDS 25.0%
!!! CATTAATAAGTCTTTTGGAA+AGG - chr4.2:27660054-27660073 MS.gene031484:intron 25.0%
AAAAAGGTTCTCTATCAAGA+AGG + chr4.2:27659619-27659638 None:intergenic 30.0%
AAGACTTATTAATGCTTGCA+TGG + chr4.2:27660048-27660067 None:intergenic 30.0%
ACATCAATGCCATTAACATT+AGG + chr4.2:27661120-27661139 None:intergenic 30.0%
AGTATATAACCCTTACAAAG+AGG - chr4.2:27660743-27660762 MS.gene031484:CDS 30.0%
CAAAAAGATCCTCTTTGTAA+GGG + chr4.2:27660755-27660774 None:intergenic 30.0%
CTATATATATCGCGAAAAAG+TGG + chr4.2:27660540-27660559 None:intergenic 30.0%
CTTTCAAATGCTTCTCTAAA+TGG + chr4.2:27659910-27659929 None:intergenic 30.0%
GAGCCTATAAAATAAAAGAG+TGG - chr4.2:27660920-27660939 MS.gene031484:CDS 30.0%
GATAAACAAGCAATATCTGT+CGG - chr4.2:27660121-27660140 MS.gene031484:intron 30.0%
TCAAAAAGATCCTCTTTGTA+AGG + chr4.2:27660756-27660775 None:intergenic 30.0%
TGTTGCATATATTCATCAAG+AGG + chr4.2:27660590-27660609 None:intergenic 30.0%
TTGTTGGATATCCAAATGTT+GGG - chr4.2:27660146-27660165 MS.gene031484:intron 30.0%
TTTATGGTGACTCATATGTA+AGG + chr4.2:27660661-27660680 None:intergenic 30.0%
TTTGTTGGATATCCAAATGT+TGG - chr4.2:27660145-27660164 MS.gene031484:intron 30.0%
! GCAAGCATTAATAAGTCTTT+TGG - chr4.2:27660049-27660068 MS.gene031484:intron 30.0%
!! ATCTTTTTGATTGATTGTCC+AGG - chr4.2:27660766-27660785 MS.gene031484:CDS 30.0%
!! GCTTAAGTGAGAATTTTGAT+TGG + chr4.2:27660443-27660462 None:intergenic 30.0%
AAATTGCAAGCACAAACACA+TGG - chr4.2:27659931-27659950 MS.gene031484:intron 35.0%
ATGATTACCAGAAGCAATCT+AGG - chr4.2:27659588-27659607 MS.gene031484:CDS 35.0%
CAAAGATGATACTTCATGAC+TGG - chr4.2:27661028-27661047 MS.gene031484:CDS 35.0%
CGACATACCATGTTTGAAAA+GGG - chr4.2:27659782-27659801 MS.gene031484:CDS 35.0%
GAAAGTAATGAAGCTGATGA+TGG - chr4.2:27659743-27659762 MS.gene031484:CDS 35.0%
GAACAAGTGTGATCTAATTC+CGG - chr4.2:27659964-27659983 MS.gene031484:intron 35.0%
TAATCAGAAGCCAAAAGACT+TGG + chr4.2:27659677-27659696 None:intergenic 35.0%
TAATCATTGAGAAGCGACAT+GGG + chr4.2:27659575-27659594 None:intergenic 35.0%
TATGAGGAGCCTAATGTTAA+TGG - chr4.2:27661108-27661127 MS.gene031484:CDS 35.0%
TCTTTAGCTAAGAAAGCTGA+TGG - chr4.2:27659701-27659720 MS.gene031484:CDS 35.0%
TGATTACCAGAAGCAATCTA+GGG - chr4.2:27659589-27659608 MS.gene031484:CDS 35.0%
! AAGAAAACGTCCAAGTCTTT+TGG - chr4.2:27659664-27659683 MS.gene031484:CDS 35.0%
! ATGATTTGCAAAGCTGAAGT+AGG + chr4.2:27660963-27660982 None:intergenic 35.0%
! ATTAGAGTGCTTTGGTTTAC+CGG + chr4.2:27659273-27659292 None:intergenic 35.0%
! TTTGATTGGTCAACTGGTAA+TGG + chr4.2:27660429-27660448 None:intergenic 35.0%
!! GGACGTTTTCTTTTAGTCTT+AGG + chr4.2:27659656-27659675 None:intergenic 35.0%
!! TAATGGCATTGATGTTGATG+AGG - chr4.2:27661125-27661144 MS.gene031484:CDS 35.0%
!!! GAGAATTTTGATTGGTCAAC+TGG + chr4.2:27660435-27660454 None:intergenic 35.0%
AAAGATGCTGCTGACCATAT+AGG - chr4.2:27660859-27660878 MS.gene031484:CDS 40.0%
AACATTAGGCTCCTCATACT+TGG + chr4.2:27661106-27661125 None:intergenic 40.0%
ACAGATGACTTCCCAACATT+TGG + chr4.2:27660160-27660179 None:intergenic 40.0%
CAACAAAGTGTACCAGTTCA+AGG - chr4.2:27661213-27661232 MS.gene031484:CDS 40.0%
CATGGAGGAGGAACAAAAAA+GGG + chr4.2:27661069-27661088 None:intergenic 40.0%
CTGTTTCAGAGTCATTGTTG+TGG + chr4.2:27660801-27660820 None:intergenic 40.0%
CTTCAGCTTTGCAAATCATC+TGG - chr4.2:27660964-27660983 MS.gene031484:CDS 40.0%
GAAACAGATGTTGTGCTGAA+GGG - chr4.2:27660814-27660833 MS.gene031484:CDS 40.0%
GCGACATACCATGTTTGAAA+AGG - chr4.2:27659781-27659800 MS.gene031484:CDS 40.0%
GCGTACTAAGAATGTTTGCA+AGG - chr4.2:27660192-27660211 MS.gene031484:intron 40.0%
GGAGTACATGGAAGTTGTTA+GGG - chr4.2:27660482-27660501 MS.gene031484:CDS 40.0%
GGTGCACGTGAATTTCTATA+AGG + chr4.2:27660569-27660588 None:intergenic 40.0%
GTAATCATTGAGAAGCGACA+TGG + chr4.2:27659576-27659595 None:intergenic 40.0%
TCATCTGGCAAACTCCTTAA+AGG - chr4.2:27660979-27660998 MS.gene031484:CDS 40.0%
TCATCTTTGCAGCAGTCATA+AGG + chr4.2:27661017-27661036 None:intergenic 40.0%
TCTCACTTAAGCGGAATTCA+TGG - chr4.2:27660451-27660470 MS.gene031484:CDS 40.0%
TCTTTCTGATTCACAACGTG+AGG + chr4.2:27659476-27659495 None:intergenic 40.0%
TGAAACAGATGTTGTGCTGA+AGG - chr4.2:27660813-27660832 MS.gene031484:CDS 40.0%
TGCTGACAAACTTCTGAACT+CGG - chr4.2:27661272-27661291 MS.gene031484:CDS 40.0%
TGGAAAGGGTTCACTTCTAT+CGG - chr4.2:27660069-27660088 MS.gene031484:intron 40.0%
TGGTAACATCTTGTACCTTG+TGG + chr4.2:27659890-27659909 None:intergenic 40.0%
TGGTATTGTACGTCTTGAGA+CGG + chr4.2:27659352-27659371 None:intergenic 40.0%
TGTGCAGGTTATAGATGCAA+GGG - chr4.2:27659862-27659881 MS.gene031484:CDS 40.0%
TTCTTCAGATGTTGTTGTGC+AGG - chr4.2:27659847-27659866 MS.gene031484:CDS 40.0%
TTGTGCAGGTTATAGATGCA+AGG - chr4.2:27659861-27659880 MS.gene031484:CDS 40.0%
! AGGAGTACATGGAAGTTGTT+AGG - chr4.2:27660481-27660500 MS.gene031484:CDS 40.0%
! GCTTTGACCCTTTTCAAACA+TGG + chr4.2:27659792-27659811 None:intergenic 40.0%
!!! CGGTTTTGAGACAATTTGCA+AGG - chr4.2:27660089-27660108 MS.gene031484:intron 40.0%
!!! GAACCTTTTTTGCATGCGTT+TGG - chr4.2:27659629-27659648 MS.gene031484:CDS 40.0%
!!! GCAATATCTGTCGGTTTTGT+TGG - chr4.2:27660130-27660149 MS.gene031484:intron 40.0%
AAGCTGTTCAGATGGAACCT+CGG + chr4.2:27661346-27661365 None:intergenic 45.0%
AGATAGCGACACATTTGAGC+AGG - chr4.2:27661311-27661330 MS.gene031484:CDS 45.0%
AGGGTCAAAGCAAACGCATA+TGG - chr4.2:27659801-27659820 MS.gene031484:CDS 45.0%
AGTGTGATCTAATTCCGGCT+TGG - chr4.2:27659969-27659988 MS.gene031484:intron 45.0%
ATACTTCATGACTGGCAGAG+GGG - chr4.2:27661036-27661055 MS.gene031484:CDS 45.0%
ATAGATGCAAGGGATCCACA+AGG - chr4.2:27659872-27659891 MS.gene031484:CDS 45.0%
CATATGGGGTGAACTCTACA+AGG - chr4.2:27659817-27659836 MS.gene031484:CDS 45.0%
CCATGGAGGAGGAACAAAAA+AGG + chr4.2:27661070-27661089 None:intergenic 45.0%
CTTACAGGTTGCTCCAATTC+CGG - chr4.2:27660702-27660721 MS.gene031484:CDS 45.0%
CTTGCATGGAATGCTAGAGT+TGG + chr4.2:27660034-27660053 None:intergenic 45.0%
GATACTGCTCCCATAAGACA+CGG + chr4.2:27659397-27659416 None:intergenic 45.0%
GATACTTCATGACTGGCAGA+GGG - chr4.2:27661035-27661054 MS.gene031484:CDS 45.0%
GCAGAAGTAAGCTGTTCAGA+TGG + chr4.2:27661354-27661373 None:intergenic 45.0%
GGGTCAAAGCAAACGCATAT+GGG - chr4.2:27659802-27659821 MS.gene031484:CDS 45.0%
GGTCAAAGCAAACGCATATG+GGG - chr4.2:27659803-27659822 MS.gene031484:CDS 45.0%
GGTCCAAACGCATGCAAAAA+AGG + chr4.2:27659635-27659654 None:intergenic 45.0%
GTGTGATCTAATTCCGGCTT+GGG - chr4.2:27659970-27659989 MS.gene031484:intron 45.0%
GTTTCAGAACCTCGCCTATA+TGG + chr4.2:27660876-27660895 None:intergenic 45.0%
GTTTGATTCTCGTGGATGGA+AGG + chr4.2:27659430-27659449 None:intergenic 45.0%
TAAGCGGAATTCATGGCGAT+GGG - chr4.2:27660458-27660477 MS.gene031484:CDS 45.0%
TCTGGCAAACTCCTTAAAGG+CGG - chr4.2:27660982-27661001 MS.gene031484:CDS 45.0%
TGACAAACTTCTGAACTCGG+AGG - chr4.2:27661275-27661294 MS.gene031484:CDS 45.0%
TGATACTTCATGACTGGCAG+AGG - chr4.2:27661034-27661053 MS.gene031484:CDS 45.0%
TGGGAGCAGTATCAACTATC+AGG - chr4.2:27659404-27659423 MS.gene031484:CDS 45.0%
TTAAGCGGAATTCATGGCGA+TGG - chr4.2:27660457-27660476 MS.gene031484:CDS 45.0%
TTACAGGTTGCTCCAATTCC+GGG - chr4.2:27660703-27660722 MS.gene031484:CDS 45.0%
TTCCGGGGGAAACAAAAGTT+TGG - chr4.2:27660719-27660738 MS.gene031484:CDS 45.0%
TTGTTGTGGTAGACAACTCC+TGG + chr4.2:27660787-27660806 None:intergenic 45.0%
TTTGCAGCAGTCATAAGGTC+AGG + chr4.2:27661012-27661031 None:intergenic 45.0%
! TTGGCGTCATTAGAGTGCTT+TGG + chr4.2:27659281-27659300 None:intergenic 45.0%
!! ACTTTTGTTTCCCCCGGAAT+TGG + chr4.2:27660718-27660737 None:intergenic 45.0%
!! GGTTCTGAAACGTGTGAAGA+AGG - chr4.2:27660885-27660904 MS.gene031484:CDS 45.0%
!! TGCCAAACTTTTGTTTCCCC+CGG + chr4.2:27660724-27660743 None:intergenic 45.0%
!!! CCTTTTTTGTTCCTCCTCCA+TGG - chr4.2:27661067-27661086 MS.gene031484:CDS 45.0%
!!! GGATTTTGGAGTCATTGCTG+CGG + chr4.2:27659304-27659323 None:intergenic 45.0%
AAACCCGATCGCCAATGGTT+CGG - chr4.2:27659446-27659465 MS.gene031484:CDS 50.0%
ATTCCCGAACCATTGGCGAT+CGG + chr4.2:27659453-27659472 None:intergenic 50.0%
CAGGCATCTGCTGCCATTAA+AGG - chr4.2:27661162-27661181 MS.gene031484:CDS 50.0%
CTTTAATGGCAGCAGATGCC+TGG + chr4.2:27661164-27661183 None:intergenic 50.0%
GAACAGCTTACTTCTGCAGC+TGG - chr4.2:27661357-27661376 MS.gene031484:CDS 50.0%
GAATCAAACCCGATCGCCAA+TGG - chr4.2:27659441-27659460 MS.gene031484:CDS 50.0%
GGAAGACCTGTCCAAGTATG+AGG - chr4.2:27661092-27661111 MS.gene031484:CDS 50.0%
GGCAACGAAAGGATGGCTTA+GGG - chr4.2:27659991-27660010 MS.gene031484:intron 50.0%
GGTGAACCCTAGATTGCTTC+TGG + chr4.2:27659598-27659617 None:intergenic 50.0%
TACAGGTTGCTCCAATTCCG+GGG - chr4.2:27660704-27660723 MS.gene031484:CDS 50.0%
TAGGCTCCTCATACTTGGAC+AGG + chr4.2:27661101-27661120 None:intergenic 50.0%
TCGGGTTTGATTCTCGTGGA+TGG + chr4.2:27659434-27659453 None:intergenic 50.0%
TGAGGCAGTTGACCATAACC+AGG - chr4.2:27661143-27661162 MS.gene031484:CDS 50.0%
! GAGAGAAGCGTGAATGTCTC+CGG - chr4.2:27659251-27659270 MS.gene031484:CDS 50.0%
! TGTTAGGGAAAGCACAGCAC+TGG - chr4.2:27660497-27660516 MS.gene031484:CDS 50.0%
! TTTGGAGTCATTGCTGCGGT+TGG + chr4.2:27659300-27659319 None:intergenic 50.0%
! TTTTGTTCCTCCTCCATGGC+TGG - chr4.2:27661071-27661090 MS.gene031484:CDS 50.0%
AAACCCGTCCGCAACCGTAA+GGG - chr4.2:27659371-27659390 MS.gene031484:CDS 55.0%
AACCCGATCGCCAATGGTTC+GGG - chr4.2:27659447-27659466 MS.gene031484:CDS 55.0%
ACAGGTTGCTCCAATTCCGG+GGG - chr4.2:27660705-27660724 MS.gene031484:CDS 55.0%
ACCGTAAGGGCCGTGTCTTA+TGG - chr4.2:27659384-27659403 MS.gene031484:CDS 55.0%
AGATGTTGTGCTGAAGGGCG+TGG - chr4.2:27660819-27660838 MS.gene031484:CDS 55.0%
CATCCTTTCGTTGCCCAAGC+CGG + chr4.2:27659986-27660005 None:intergenic 55.0%
CCCATAAGACACGGCCCTTA+CGG + chr4.2:27659388-27659407 None:intergenic 55.0%
CCGTAAGGGCCGTGTCTTAT+GGG - chr4.2:27659385-27659404 MS.gene031484:CDS 55.0%
CGTGAGGATTCCCGAACCAT+TGG + chr4.2:27659460-27659479 None:intergenic 55.0%
CTGTTCAGATGGAACCTCGG+TGG + chr4.2:27661343-27661362 None:intergenic 55.0%
GCGATCGGGTTTGATTCTCG+TGG + chr4.2:27659438-27659457 None:intergenic 55.0%
GGGCAACGAAAGGATGGCTT+AGG - chr4.2:27659990-27660009 MS.gene031484:intron 55.0%
GGTCAGGTTCACCGCCTTTA+AGG + chr4.2:27660996-27661015 None:intergenic 55.0%
TCAGATGGAACCTCGGTGGA+TGG + chr4.2:27661339-27661358 None:intergenic 55.0%
TGCTGCTGACCATATAGGCG+AGG - chr4.2:27660864-27660883 MS.gene031484:CDS 55.0%
TGGCAGCAGATGCCTGGTTA+TGG + chr4.2:27661158-27661177 None:intergenic 55.0%
TTCCCGAACCATTGGCGATC+GGG + chr4.2:27659452-27659471 None:intergenic 55.0%
TTGGACAGGTCTTCCAGCCA+TGG + chr4.2:27661087-27661106 None:intergenic 55.0%
! ATGGCGATGGGAGGAGTACA+TGG - chr4.2:27660470-27660489 MS.gene031484:CDS 55.0%
! GGCTGAGCGTGACTGGATTT+TGG + chr4.2:27659318-27659337 None:intergenic 55.0%
!! ATTCCGGCTTGGGCAACGAA+AGG - chr4.2:27659980-27659999 MS.gene031484:intron 55.0%
AGACACGGCCCTTACGGTTG+CGG + chr4.2:27659382-27659401 None:intergenic 60.0%
AGGTCTTCCAGCCATGGAGG+AGG + chr4.2:27661081-27661100 None:intergenic 60.0%
CAAACCCGTCCGCAACCGTA+AGG - chr4.2:27659370-27659389 MS.gene031484:CDS 60.0%
CGTCTTGAGACGGCGAACAG+TGG + chr4.2:27659342-27659361 None:intergenic 60.0%
CTTGAGACGGCGAACAGTGG+CGG + chr4.2:27659339-27659358 None:intergenic 60.0%
GACAGGTCTTCCAGCCATGG+AGG + chr4.2:27661084-27661103 None:intergenic 60.0%
GCGGAATTCATGGCGATGGG+AGG - chr4.2:27660461-27660480 MS.gene031484:CDS 60.0%
TGAGCAGGATCCATCCACCG+AGG - chr4.2:27661326-27661345 MS.gene031484:CDS 60.0%
! CGGCTTGGGCAACGAAAGGA+TGG - chr4.2:27659984-27660003 MS.gene031484:intron 60.0%
! CTTACGGTTGCGGACGGGTT+TGG + chr4.2:27659372-27659391 None:intergenic 60.0%
ACGGCCCTTACGGTTGCGGA+CGG + chr4.2:27659378-27659397 None:intergenic 65.0%
CAGTGGCGGCTGAGCGTGAC+TGG + chr4.2:27659325-27659344 None:intergenic 70.0%
CGGCCCTTACGGTTGCGGAC+GGG + chr4.2:27659377-27659396 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 27659236 27661392 27659236 ID=MS.gene031484
chr4.2 mRNA 27659236 27661392 27659236 ID=MS.gene031484.t1;Parent=MS.gene031484
chr4.2 exon 27660415 27661392 27660415 ID=MS.gene031484.t1.exon1;Parent=MS.gene031484.t1
chr4.2 CDS 27660415 27661392 27660415 ID=cds.MS.gene031484.t1;Parent=MS.gene031484.t1
chr4.2 exon 27659236 27659919 27659236 ID=MS.gene031484.t1.exon2;Parent=MS.gene031484.t1
chr4.2 CDS 27659236 27659919 27659236 ID=cds.MS.gene031484.t1;Parent=MS.gene031484.t1
Gene Sequence

>MS.gene031484

ATGGCGAAGAAGAGAGAGAGAAGCGTGAATGTCTCCGGTAAACCAAAGCACTCTAATGACGCCAACCGCAGCAATGACTCCAAAATCCAGTCACGCTCAGCCGCCACTGTTCGCCGTCTCAAGACGTACAATACCAAACCCGTCCGCAACCGTAAGGGCCGTGTCTTATGGGAGCAGTATCAACTATCAGGCCTTCCATCCACGAGAATCAAACCCGATCGCCAATGGTTCGGGAATCCTCACGTTGTGAATCAGAAAGAGCTAGAGTTTTTTAGAGAAGAAATGGCGAAGCATAGTTCGAGTAATTATAATGTTATCATCAAGGGGAAGAATTTACCCATGTCGCTTCTCAATGATTACCAGAAGCAATCTAGGGTTCACCTTCTTGATAGAGAACCTTTTTTGCATGCGTTTGGACCTAAGACTAAAAGAAAACGTCCAAGTCTTTTGGCTTCTGATTATGAGTCTTTAGCTAAGAAAGCTGATGGTTCTCAAGATGCTTTTGAAGAAAGTAATGAAGCTGATGATGGATTTAGAGATTTAGTGCGACATACCATGTTTGAAAAGGGTCAAAGCAAACGCATATGGGGTGAACTCTACAAGGTGATAGATTCTTCAGATGTTGTTGTGCAGGTTATAGATGCAAGGGATCCACAAGGTACAAGATGTTACCATTTAGAGAAGCATTTGAAAGAAAATTGCAAGCACAAACACATGGTGCTTTTATTGAACAAGTGTGATCTAATTCCGGCTTGGGCAACGAAAGGATGGCTTAGGGTTTTGTCAAAAGAATATCCAACTCTAGCATTCCATGCAAGCATTAATAAGTCTTTTGGAAAGGGTTCACTTCTATCGGTTTTGAGACAATTTGCAAGGTTCAAAAGTGATAAACAAGCAATATCTGTCGGTTTTGTTGGATATCCAAATGTTGGGAAGTCATCTGTGATCAATACATTGCGTACTAAGAATGTTTGCAAGGTTGTGTAAGTTTTAGTTTTCATAGTCATGTTTACATTCTAGAAAATTAACACATTTGATCTTGACATATATGATTCTCACATATTCGTTCTTGATGATAATATGATAGAATCCTTTGTGCATTAGTTAGTAATTTCATCATGTTACATGTAATTATAGAAAGAGGTGTGCTATATCAAGCCAAAAAATTATAGCGAATGTTTCACGAATTACCATTACCAGTTGACCAATCAAAATTCTCACTTAAGCGGAATTCATGGCGATGGGAGGAGTACATGGAAGTTGTTAGGGAAAGCACAGCACTGGTGAAACAATGTCGCGAGCCACTTTTTCGCGATATATATAGCTTTATCCTTATAGAAATTCACGTGCACCTCTTGATGAATATATGCAACATTTCACATGAACATTTGCATCCTTTCACATTCACATGAATAATTGCATCCTTACATATGAGTCACCATAAAGCAATTATATTATTAATTATTCTTACAGGTTGCTCCAATTCCGGGGGAAACAAAAGTTTGGCAGTATATAACCCTTACAAAGAGGATCTTTTTGATTGATTGTCCAGGAGTTGTCTACCACAACAATGACTCTGAAACAGATGTTGTGCTGAAGGGCGTGGTACGTGTAACAAACTTGAAAGATGCTGCTGACCATATAGGCGAGGTTCTGAAACGTGTGAAGAAGGAGCACTTGACAAGAGCCTATAAAATAAAAGAGTGGGTTGATGAAAATGACTTCCTACTTCAGCTTTGCAAATCATCTGGCAAACTCCTTAAAGGCGGTGAACCTGACCTTATGACTGCTGCAAAGATGATACTTCATGACTGGCAGAGGGGTAAAATTCCCTTTTTTGTTCCTCCTCCATGGCTGGAAGACCTGTCCAAGTATGAGGAGCCTAATGTTAATGGCATTGATGTTGATGAGGCAGTTGACCATAACCAGGCATCTGCTGCCATTAAAGGTATTGCAAATATTCTTTCGTCGCAGCATCAACAAAGTGTACCAGTTCAAGGTGATTTATATAATGAGAATGAGTTAAAGGGAGAAACTGCTGACAAACTTCTGAACTCGGAGGATGATACAGATGAAGATAGCGACACATTTGAGCAGGATCCATCCACCGAGGTTCCATCTGAACAGCTTACTTCTGCAGCTGGTCCCAACAAATGA
Protein sequence

>MS.gene031484.t1

MAKKRERSVNVSGKPKHSNDANRSNDSKIQSRSAATVRRLKTYNTKPVRNRKGRVLWEQYQLSGLPSTRIKPDRQWFGNPHVVNQKELEFFREEMAKHSSSNYNVIIKGKNLPMSLLNDYQKQSRVHLLDREPFLHAFGPKTKRKRPSLLASDYESLAKKADGSQDAFEESNEADDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVIDARDPQGTRCYHLEKHLKENCKHKHMVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFARFKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPGVVYHNNDSETDVVLKGVVRVTNLKDAADHIGEVLKRVKKEHLTRAYKIKEWVDENDFLLQLCKSSGKLLKGGEPDLMTAAKMILHDWQRGKIPFFVPPPWLEDLSKYEEPNVNGIDVDEAVDHNQASAAIKGIANILSSQHQQSVPVQGDLYNENELKGETADKLLNSEDDTDEDSDTFEQDPSTEVPSEQLTSAAGPNK