AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053790


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053790.t1 MTR_8g101980 86.777 121 1 1 1 106 1 121 1.02e-68 201
MS.gene053790.t1 MTR_6g091630 84.298 121 4 1 1 106 1 121 1.20e-66 196
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053790.t1 AT5G56710 70.248 121 19 2 1 106 1 119 4.46e-53 162
MS.gene053790.t1 AT4G26230 69.421 121 20 2 1 106 1 119 1.54e-52 160
MS.gene053790.t1 AT2G19740 69.828 116 20 1 6 106 4 119 8.90e-52 159
MS.gene053790.t1 AT5G56710 59.434 106 22 2 1 106 1 85 1.24e-34 114

Find 45 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGATCTCCTTTATTGCTTT+AGG 0.186329 5.1:-44983292 None:intergenic
AGTTTGCCCAAAAGGCTATT+TGG 0.248252 5.1:+44983317 MS.gene053790:CDS
CAAGCCTTTGAGTTCTTCCT+TGG 0.279778 5.1:-44983455 None:intergenic
CCTGGCTCCAAATAGCCTTT+TGG 0.287456 5.1:-44983324 None:intergenic
GAAAGCTCCTAAAGCAATAA+AGG 0.311659 5.1:+44983285 MS.gene053790:CDS
CTCATCATCGTTCCTCTTGC+GGG 0.315756 5.1:-44983386 None:intergenic
GAAGTGTCCCAAGGAGGGTT+AGG 0.369762 5.1:+44983353 MS.gene053790:CDS
AAGCCTTTGAGTTCTTCCTT+GGG 0.394369 5.1:-44983454 None:intergenic
GAGGCGTTTGTGGAGGTTGA+TGG 0.411978 5.1:-44983242 None:intergenic
GATCAGGAAGTTTGCCCAAA+AGG 0.422618 5.1:+44983309 MS.gene053790:CDS
TTGGGACACTTCTGATCCCC+TGG 0.423986 5.1:-44983342 None:intergenic
ATGCGTACCCTAACCCTCCT+TGG 0.424283 5.1:-44983361 None:intergenic
CTAAAGCAATAAAGGAGATC+AGG 0.445899 5.1:+44983293 MS.gene053790:CDS
CAAGGAAGAACTCAAAGGCT+TGG 0.450797 5.1:+44983456 MS.gene053790:CDS
CCAAAAGGCTATTTGGAGCC+AGG 0.455966 5.1:+44983324 MS.gene053790:CDS
TGCAGCCATGGAGGCGTTTG+TGG 0.456342 5.1:-44983252 None:intergenic
CCTCATCATCGTTCCTCTTG+CGG 0.459607 5.1:-44983387 None:intergenic
TGCGTACCCTAACCCTCCTT+GGG 0.479198 5.1:-44983360 None:intergenic
AAGGAAGAACTCAAAGGCTT+GGG 0.487684 5.1:+44983457 MS.gene053790:CDS
GTGAAAATGGTGGAGAAGAC+CGG 0.499433 5.1:+44983181 None:intergenic
GAGGTTGATGGTGTACTCTC+TGG 0.499941 5.1:-44983230 None:intergenic
GACAACCTCTTCCTTCCTAC+TGG 0.501117 5.1:-44983206 None:intergenic
AAGTGTCCCAAGGAGGGTTA+GGG 0.502241 5.1:+44983354 MS.gene053790:CDS
CTCTTCCTTCCTACTGGCGC+CGG 0.517625 5.1:-44983200 None:intergenic
CGGCGCCAGTAGGAAGGAAG+AGG 0.519836 5.1:+44983201 MS.gene053790:CDS
TCTTCTTAAATGTGCAGCCA+TGG 0.524032 5.1:-44983264 None:intergenic
AGCCTTTGAGTTCTTCCTTG+GGG 0.534682 5.1:-44983453 None:intergenic
CACAAAGGTTATTGATGATG+AGG 0.542190 5.1:+44983480 MS.gene053790:CDS
GAACGATGATGAGGATGCTA+AGG 0.543980 5.1:+44983396 MS.gene053790:CDS
CAAAAGGCTATTTGGAGCCA+GGG 0.555648 5.1:+44983325 MS.gene053790:CDS
GAAGACCGGCGCCAGTAGGA+AGG 0.555962 5.1:+44983195 MS.gene053790:CDS
AACCTCCACAAACGCCTCCA+TGG 0.560698 5.1:+44983247 MS.gene053790:CDS
TTCCTTGGGGATTTCAACAA+CGG 0.577011 5.1:-44983440 None:intergenic
TGGAGAAGACCGGCGCCAGT+AGG 0.607373 5.1:+44983191 MS.gene053790:CDS
CCGCAAGAGGAACGATGATG+AGG 0.607958 5.1:+44983387 MS.gene053790:CDS
GATCAGAAGTGTCCCAAGGA+GGG 0.621367 5.1:+44983348 MS.gene053790:CDS
GGGTACGCATTGCCCGCAAG+AGG 0.627432 5.1:+44983374 MS.gene053790:CDS
TACCGTTGTTGAAATCCCCA+AGG 0.634406 5.1:+44983438 MS.gene053790:CDS
AGCCATGGAGGCGTTTGTGG+AGG 0.637282 5.1:-44983249 None:intergenic
AGGGGATCAGAAGTGTCCCA+AGG 0.639457 5.1:+44983344 MS.gene053790:CDS
ACTCAAAGGCTTGGGCACAA+AGG 0.642962 5.1:+44983465 MS.gene053790:CDS
ATCCCCAAGGAAGAACTCAA+AGG 0.650756 5.1:+44983451 MS.gene053790:CDS
AAAAGGCTATTTGGAGCCAG+GGG 0.653360 5.1:+44983326 MS.gene053790:CDS
TCTTAAATGTGCAGCCATGG+AGG 0.665182 5.1:-44983261 None:intergenic
GGATCAGAAGTGTCCCAAGG+AGG 0.680908 5.1:+44983347 MS.gene053790:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CTAAAGCAATAAAGGAGATC+AGG + chr5.1:44983293-44983312 MS.gene053790:CDS 35.0%
CTGATCTCCTTTATTGCTTT+AGG - chr5.1:44983295-44983314 None:intergenic 35.0%
GAAAGCTCCTAAAGCAATAA+AGG + chr5.1:44983285-44983304 MS.gene053790:CDS 35.0%
!! CACAAAGGTTATTGATGATG+AGG + chr5.1:44983480-44983499 MS.gene053790:CDS 35.0%
AAGGAAGAACTCAAAGGCTT+GGG + chr5.1:44983457-44983476 MS.gene053790:CDS 40.0%
AGTTTGCCCAAAAGGCTATT+TGG + chr5.1:44983317-44983336 MS.gene053790:CDS 40.0%
TCTTCTTAAATGTGCAGCCA+TGG - chr5.1:44983267-44983286 None:intergenic 40.0%
TTCCTTGGGGATTTCAACAA+CGG - chr5.1:44983443-44983462 None:intergenic 40.0%
! AAGCCTTTGAGTTCTTCCTT+GGG - chr5.1:44983457-44983476 None:intergenic 40.0%
ATCCCCAAGGAAGAACTCAA+AGG + chr5.1:44983451-44983470 MS.gene053790:CDS 45.0%
CAAGGAAGAACTCAAAGGCT+TGG + chr5.1:44983456-44983475 MS.gene053790:CDS 45.0%
GAACGATGATGAGGATGCTA+AGG + chr5.1:44983396-44983415 MS.gene053790:CDS 45.0%
GATCAGGAAGTTTGCCCAAA+AGG + chr5.1:44983309-44983328 MS.gene053790:CDS 45.0%
TACCGTTGTTGAAATCCCCA+AGG + chr5.1:44983438-44983457 MS.gene053790:CDS 45.0%
TCTTAAATGTGCAGCCATGG+AGG - chr5.1:44983264-44983283 None:intergenic 45.0%
! AAAAGGCTATTTGGAGCCAG+GGG + chr5.1:44983326-44983345 MS.gene053790:CDS 45.0%
! AGCCTTTGAGTTCTTCCTTG+GGG - chr5.1:44983456-44983475 None:intergenic 45.0%
! CAAAAGGCTATTTGGAGCCA+GGG + chr5.1:44983325-44983344 MS.gene053790:CDS 45.0%
! CAAGCCTTTGAGTTCTTCCT+TGG - chr5.1:44983458-44983477 None:intergenic 45.0%
! CTGGCTCCAAATAGCCTTTT+GGG - chr5.1:44983326-44983345 None:intergenic 45.0%
AAGTGTCCCAAGGAGGGTTA+GGG + chr5.1:44983354-44983373 MS.gene053790:CDS 50.0%
ACTCAAAGGCTTGGGCACAA+AGG + chr5.1:44983465-44983484 MS.gene053790:CDS 50.0%
CCTCATCATCGTTCCTCTTG+CGG - chr5.1:44983390-44983409 None:intergenic 50.0%
CTCATCATCGTTCCTCTTGC+GGG - chr5.1:44983389-44983408 None:intergenic 50.0%
GACAACCTCTTCCTTCCTAC+TGG - chr5.1:44983209-44983228 None:intergenic 50.0%
! CCAAAAGGCTATTTGGAGCC+AGG + chr5.1:44983324-44983343 MS.gene053790:CDS 50.0%
! CCTGGCTCCAAATAGCCTTT+TGG - chr5.1:44983327-44983346 None:intergenic 50.0%
!! GAGGTTGATGGTGTACTCTC+TGG - chr5.1:44983233-44983252 None:intergenic 50.0%
!! GATCAGAAGTGTCCCAAGGA+GGG + chr5.1:44983348-44983367 MS.gene053790:CDS 50.0%
AACCTCCACAAACGCCTCCA+TGG + chr5.1:44983247-44983266 MS.gene053790:CDS 55.0%
ATGCGTACCCTAACCCTCCT+TGG - chr5.1:44983364-44983383 None:intergenic 55.0%
CCGCAAGAGGAACGATGATG+AGG + chr5.1:44983387-44983406 MS.gene053790:CDS 55.0%
TGCGTACCCTAACCCTCCTT+GGG - chr5.1:44983363-44983382 None:intergenic 55.0%
TTGGGACACTTCTGATCCCC+TGG - chr5.1:44983345-44983364 None:intergenic 55.0%
! GAAGTGTCCCAAGGAGGGTT+AGG + chr5.1:44983353-44983372 MS.gene053790:CDS 55.0%
! GAGGCGTTTGTGGAGGTTGA+TGG - chr5.1:44983245-44983264 None:intergenic 55.0%
!! AGGGGATCAGAAGTGTCCCA+AGG + chr5.1:44983344-44983363 MS.gene053790:CDS 55.0%
!! GGATCAGAAGTGTCCCAAGG+AGG + chr5.1:44983347-44983366 MS.gene053790:CDS 55.0%
CTCTTCCTTCCTACTGGCGC+CGG - chr5.1:44983203-44983222 None:intergenic 60.0%
!! AGCCATGGAGGCGTTTGTGG+AGG - chr5.1:44983252-44983271 None:intergenic 60.0%
!! TGCAGCCATGGAGGCGTTTG+TGG - chr5.1:44983255-44983274 None:intergenic 60.0%
CGGCGCCAGTAGGAAGGAAG+AGG + chr5.1:44983201-44983220 MS.gene053790:CDS 65.0%
GAAGACCGGCGCCAGTAGGA+AGG + chr5.1:44983195-44983214 MS.gene053790:CDS 65.0%
GGGTACGCATTGCCCGCAAG+AGG + chr5.1:44983374-44983393 MS.gene053790:CDS 65.0%
TGGAGAAGACCGGCGCCAGT+AGG + chr5.1:44983191-44983210 MS.gene053790:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 44983187 44983507 44983187 ID=MS.gene053790
chr5.1 mRNA 44983187 44983507 44983187 ID=MS.gene053790.t1;Parent=MS.gene053790
chr5.1 exon 44983187 44983507 44983187 ID=MS.gene053790.t1.exon1;Parent=MS.gene053790.t1
chr5.1 CDS 44983187 44983507 44983187 ID=cds.MS.gene053790.t1;Parent=MS.gene053790.t1
Gene Sequence

>MS.gene053790

ATGGTGGAGAAGACCGGCGCCAGTAGGAAGGAAGAGGTTGTCACCAGAGAGTACACCATCAACCTCCACAAACGCCTCCATGGCTGCACATTTAAGAAGAAAGCTCCTAAAGCAATAAAGGAGATCAGGAAGTTTGCCCAAAAGGCTATTTGGAGCCAGGGGATCAGAAGTGTCCCAAGGAGGGTTAGGGTACGCATTGCCCGCAAGAGGAACGATGATGAGGATGCTAAGGAAGAGCTCTACTCACTTGTTACCGTTGTTGAAATCCCCAAGGAAGAACTCAAAGGCTTGGGCACAAAGGTTATTGATGATGAGGATTGA

Protein sequence

>MS.gene053790.t1

MVEKTGASRKEEVVTREYTINLHKRLHGCTFKKKAPKAIKEIRKFAQKAIWSQGIRSVPRRVRVRIARKRNDDEDAKEELYSLVTVVEIPKEELKGLGTKVIDDED