AlfalfaGEDB | MS.gene99599

M. sativa cultivar XinJiangDaYe / MS.gene99599

KEGG: K02910
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01198.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99599.t1	XP_003630662.2	99.2	121	1	0	1	121	1	121	5.50E-58	233.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99599.t1	P46290	86.8	121	15	1	1	121	1	120	9.5e-51	200.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99599.t1	B7FGL4	99.2	121	1	0	1	121	1	121	4.0e-58	233.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049876	MS.gene99599	0.802217	6.23E-49	-1.69E-46
MS.gene050290	MS.gene99599	0.807992	3.85E-50	-1.69E-46
MS.gene050916	MS.gene99599	0.825635	4.21E-54	-1.69E-46
MS.gene054937	MS.gene99599	0.820717	5.92E-53	-1.69E-46
MS.gene056257	MS.gene99599	0.843896	1.06E-58	-1.69E-46
MS.gene056917	MS.gene99599	0.828329	9.55E-55	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene049030	MS.gene99599	PPI
MS.gene006800	MS.gene99599	PPI
MS.gene065652	MS.gene99599	PPI
MS.gene068781	MS.gene99599	PPI
MS.gene053790	MS.gene99599	PPI
MS.gene012331	MS.gene99599	PPI
MS.gene75054	MS.gene99599	PPI
MS.gene99599	MS.gene96780	PPI
MS.gene55890	MS.gene99599	PPI
MS.gene96312	MS.gene99599	PPI
MS.gene99599	MS.gene064028	PPI
MS.gene99599	MS.gene030304	PPI
MS.gene99599	MS.gene75054	PPI
MS.gene92970	MS.gene99599	PPI
MS.gene99599	MS.gene09046	PPI
MS.gene012333	MS.gene99599	PPI
MS.gene059846	MS.gene99599	PPI
MS.gene09188	MS.gene99599	PPI
MS.gene99599	MS.gene56525	PPI
MS.gene065189	MS.gene99599	PPI
MS.gene99599	MS.gene08259	PPI
MS.gene09187	MS.gene99599	PPI
MS.gene99599	MS.gene031484	PPI
MS.gene08260	MS.gene99599	PPI
MS.gene66352	MS.gene99599	PPI
MS.gene029547	MS.gene99599	PPI
MS.gene06169	MS.gene99599	PPI
MS.gene064028	MS.gene99599	PPI
MS.gene073324	MS.gene99599	PPI
MS.gene023381	MS.gene99599	PPI
MS.gene031406	MS.gene99599	PPI
MS.gene20153	MS.gene99599	PPI
MS.gene57660	MS.gene99599	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99599.t1	MTR_8g101980	99.174	121	1	0	1	121	1	121	1.34e-83	240
MS.gene99599.t1	MTR_6g091630	96.694	121	4	0	1	121	1	121	1.64e-81	234

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99599.t1	AT5G56710	82.456	114	20	0	8	121	6	119	1.22e-65	194
MS.gene99599.t1	AT4G26230	81.579	114	21	0	8	121	6	119	4.70e-65	193
MS.gene99599.t1	AT2G19740	81.034	116	22	0	6	121	4	119	6.75e-65	192
MS.gene99599.t1	AT5G56710	57.895	114	14	1	8	121	6	85	1.11e-36	120

Find 49 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGATCTCCTTTATTGCTTT+AGG	0.186329	5.4:-45406661	None:intergenic
TCAAGTTGAACAAGGCTATT+TGG	0.229692	5.4:+45406731	MS.gene99599:CDS
CATTAGTTCCCATAGCCTTT+TGG	0.252576	5.4:-45406693	None:intergenic
CAAGCCTTTGAGTTCTTCCT+TGG	0.279778	5.4:-45406869	None:intergenic
GAAAGCTCCTAAAGCAATAA+AGG	0.311659	5.4:+45406654	MS.gene99599:CDS
CTCATCATCGTTCCTCTTGC+GGG	0.315756	5.4:-45406800	None:intergenic
GAAGTGTCCCAAGGAGGGTT+AGG	0.369762	5.4:+45406767	MS.gene99599:CDS
AAGCCTTTGAGTTCTTCCTT+GGG	0.394369	5.4:-45406868	None:intergenic
GAGGCGTTTGTGGAGGTTGA+TGG	0.411978	5.4:-45406611	None:intergenic
GATCAGGAAGTTTGCCCAAA+AGG	0.422618	5.4:+45406678	MS.gene99599:CDS
TTGGGACACTTCTGATCCCC+TGG	0.423986	5.4:-45406756	None:intergenic
ATGCGTACCCTAACCCTCCT+TGG	0.424283	5.4:-45406775	None:intergenic
CTAAAGCAATAAAGGAGATC+AGG	0.445899	5.4:+45406662	MS.gene99599:CDS
CAAGGAAGAACTCAAAGGCT+TGG	0.450797	5.4:+45406870	MS.gene99599:CDS
TGCAGCCATGGAGGCGTTTG+TGG	0.456342	5.4:-45406621	None:intergenic
CCTCATCATCGTTCCTCTTG+CGG	0.459607	5.4:-45406801	None:intergenic
GAACAAGGCTATTTGGAGCC+AGG	0.460263	5.4:+45406738	MS.gene99599:CDS
TGCGTACCCTAACCCTCCTT+GGG	0.479198	5.4:-45406774	None:intergenic
CTCTTCCTTCCTACCGGCGC+CGG	0.482937	5.4:-45406569	None:intergenic
GAAGTTTGCCCAAAAGGCTA+TGG	0.483900	5.4:+45406684	MS.gene99599:CDS
AAGGAAGAACTCAAAGGCTT+GGG	0.487684	5.4:+45406871	MS.gene99599:CDS
GTGAAAATGGTGGAGAAGAC+CGG	0.499433	5.4:+45406550	None:intergenic
GAGGTTGATGGTGTACTCTC+TGG	0.499941	5.4:-45406599	None:intergenic
AAGTGTCCCAAGGAGGGTTA+GGG	0.502241	5.4:+45406768	MS.gene99599:CDS
TCTTCTTAAATGTGCAGCCA+TGG	0.524032	5.4:-45406633	None:intergenic
AACAAGGCTATTTGGAGCCA+GGG	0.528204	5.4:+45406739	MS.gene99599:CDS
AGCCTTTGAGTTCTTCCTTG+GGG	0.534682	5.4:-45406867	None:intergenic
CACAAAGGTTATTGATGATG+AGG	0.542190	5.4:+45406894	MS.gene99599:CDS
GAACGATGATGAGGATGCTA+AGG	0.543980	5.4:+45406810	MS.gene99599:CDS
GAAGACCGGCGCCGGTAGGA+AGG	0.546764	5.4:+45406564	MS.gene99599:CDS
CGGCGCCGGTAGGAAGGAAG+AGG	0.547239	5.4:+45406570	MS.gene99599:CDS
AACCTCCACAAACGCCTCCA+TGG	0.560698	5.4:+45406616	MS.gene99599:CDS
TTCCTTGGGGATTTCAACAA+CGG	0.577011	5.4:-45406854	None:intergenic
GACAACCTCTTCCTTCCTAC+CGG	0.582542	5.4:-45406575	None:intergenic
AAGTTTGCCCAAAAGGCTAT+GGG	0.584767	5.4:+45406685	MS.gene99599:CDS
CCGCAAGAGGAACGATGATG+AGG	0.607958	5.4:+45406801	MS.gene99599:CDS
TGGAGAAGACCGGCGCCGGT+AGG	0.608791	5.4:+45406560	MS.gene99599:CDS
ATGGTGGAGAAGACCGGCGC+CGG	0.610511	5.4:+45406556	MS.gene99599:CDS
GATCAGAAGTGTCCCAAGGA+GGG	0.621367	5.4:+45406762	MS.gene99599:CDS
GGGTACGCATTGCCCGCAAG+AGG	0.627432	5.4:+45406788	MS.gene99599:CDS
TACCGTTGTTGAAATCCCCA+AGG	0.634406	5.4:+45406852	MS.gene99599:CDS
AGCCATGGAGGCGTTTGTGG+AGG	0.637282	5.4:-45406618	None:intergenic
AGGGGATCAGAAGTGTCCCA+AGG	0.639457	5.4:+45406758	MS.gene99599:CDS
ACTCAAAGGCTTGGGCACAA+AGG	0.642962	5.4:+45406879	MS.gene99599:CDS
ACAAGGCTATTTGGAGCCAG+GGG	0.649532	5.4:+45406740	MS.gene99599:CDS
ATCCCCAAGGAAGAACTCAA+AGG	0.650756	5.4:+45406865	MS.gene99599:CDS
TCTTAAATGTGCAGCCATGG+AGG	0.665182	5.4:-45406630	None:intergenic
AGTGTATGTCAAGTTGAACA+AGG	0.670619	5.4:+45406723	MS.gene99599:CDS
GGATCAGAAGTGTCCCAAGG+AGG	0.680908	5.4:+45406761	MS.gene99599:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTAAAGCAATAAAGGAGATC+AGG	+	chr5.4:45406662-45406681	MS.gene99599:CDS	35.0%
	CTGATCTCCTTTATTGCTTT+AGG	-	chr5.4:45406664-45406683	None:intergenic	35.0%
	GAAAGCTCCTAAAGCAATAA+AGG	+	chr5.4:45406654-45406673	MS.gene99599:CDS	35.0%
!	ATTAGTTCCCATAGCCTTTT+GGG	-	chr5.4:45406695-45406714	None:intergenic	35.0%
!!	AGTGTATGTCAAGTTGAACA+AGG	+	chr5.4:45406723-45406742	MS.gene99599:CDS	35.0%
!!	CACAAAGGTTATTGATGATG+AGG	+	chr5.4:45406894-45406913	MS.gene99599:CDS	35.0%
!!	TCAAGTTGAACAAGGCTATT+TGG	+	chr5.4:45406731-45406750	MS.gene99599:CDS	35.0%
	AAGGAAGAACTCAAAGGCTT+GGG	+	chr5.4:45406871-45406890	MS.gene99599:CDS	40.0%
	AAGTTTGCCCAAAAGGCTAT+GGG	+	chr5.4:45406685-45406704	MS.gene99599:CDS	40.0%
	TCTTCTTAAATGTGCAGCCA+TGG	-	chr5.4:45406636-45406655	None:intergenic	40.0%
	TTCCTTGGGGATTTCAACAA+CGG	-	chr5.4:45406857-45406876	None:intergenic	40.0%
!	AAGCCTTTGAGTTCTTCCTT+GGG	-	chr5.4:45406871-45406890	None:intergenic	40.0%
!	CATTAGTTCCCATAGCCTTT+TGG	-	chr5.4:45406696-45406715	None:intergenic	40.0%
	ATCCCCAAGGAAGAACTCAA+AGG	+	chr5.4:45406865-45406884	MS.gene99599:CDS	45.0%
	CAAGGAAGAACTCAAAGGCT+TGG	+	chr5.4:45406870-45406889	MS.gene99599:CDS	45.0%
	GAACGATGATGAGGATGCTA+AGG	+	chr5.4:45406810-45406829	MS.gene99599:CDS	45.0%
	GAAGTTTGCCCAAAAGGCTA+TGG	+	chr5.4:45406684-45406703	MS.gene99599:CDS	45.0%
	GATCAGGAAGTTTGCCCAAA+AGG	+	chr5.4:45406678-45406697	MS.gene99599:CDS	45.0%
	TACCGTTGTTGAAATCCCCA+AGG	+	chr5.4:45406852-45406871	MS.gene99599:CDS	45.0%
	TCTTAAATGTGCAGCCATGG+AGG	-	chr5.4:45406633-45406652	None:intergenic	45.0%
!	AACAAGGCTATTTGGAGCCA+GGG	+	chr5.4:45406739-45406758	MS.gene99599:CDS	45.0%
!	AGCCTTTGAGTTCTTCCTTG+GGG	-	chr5.4:45406870-45406889	None:intergenic	45.0%
!	CAAGCCTTTGAGTTCTTCCT+TGG	-	chr5.4:45406872-45406891	None:intergenic	45.0%
	AAGTGTCCCAAGGAGGGTTA+GGG	+	chr5.4:45406768-45406787	MS.gene99599:CDS	50.0%
	ACTCAAAGGCTTGGGCACAA+AGG	+	chr5.4:45406879-45406898	MS.gene99599:CDS	50.0%
	CCTCATCATCGTTCCTCTTG+CGG	-	chr5.4:45406804-45406823	None:intergenic	50.0%
	CTCATCATCGTTCCTCTTGC+GGG	-	chr5.4:45406803-45406822	None:intergenic	50.0%
	GACAACCTCTTCCTTCCTAC+CGG	-	chr5.4:45406578-45406597	None:intergenic	50.0%
!	ACAAGGCTATTTGGAGCCAG+GGG	+	chr5.4:45406740-45406759	MS.gene99599:CDS	50.0%
!	GAACAAGGCTATTTGGAGCC+AGG	+	chr5.4:45406738-45406757	MS.gene99599:CDS	50.0%
!!	GAGGTTGATGGTGTACTCTC+TGG	-	chr5.4:45406602-45406621	None:intergenic	50.0%
!!	GATCAGAAGTGTCCCAAGGA+GGG	+	chr5.4:45406762-45406781	MS.gene99599:CDS	50.0%
	AACCTCCACAAACGCCTCCA+TGG	+	chr5.4:45406616-45406635	MS.gene99599:CDS	55.0%
	ATGCGTACCCTAACCCTCCT+TGG	-	chr5.4:45406778-45406797	None:intergenic	55.0%
	CCGCAAGAGGAACGATGATG+AGG	+	chr5.4:45406801-45406820	MS.gene99599:CDS	55.0%
	TGCGTACCCTAACCCTCCTT+GGG	-	chr5.4:45406777-45406796	None:intergenic	55.0%
	TTGGGACACTTCTGATCCCC+TGG	-	chr5.4:45406759-45406778	None:intergenic	55.0%
!	GAAGTGTCCCAAGGAGGGTT+AGG	+	chr5.4:45406767-45406786	MS.gene99599:CDS	55.0%
!	GAGGCGTTTGTGGAGGTTGA+TGG	-	chr5.4:45406614-45406633	None:intergenic	55.0%
!!	AGGGGATCAGAAGTGTCCCA+AGG	+	chr5.4:45406758-45406777	MS.gene99599:CDS	55.0%
!!	GGATCAGAAGTGTCCCAAGG+AGG	+	chr5.4:45406761-45406780	MS.gene99599:CDS	55.0%
!!	AGCCATGGAGGCGTTTGTGG+AGG	-	chr5.4:45406621-45406640	None:intergenic	60.0%
!!	TGCAGCCATGGAGGCGTTTG+TGG	-	chr5.4:45406624-45406643	None:intergenic	60.0%
	CTCTTCCTTCCTACCGGCGC+CGG	-	chr5.4:45406572-45406591	None:intergenic	65.0%
	GGGTACGCATTGCCCGCAAG+AGG	+	chr5.4:45406788-45406807	MS.gene99599:CDS	65.0%
!	ATGGTGGAGAAGACCGGCGC+CGG	+	chr5.4:45406556-45406575	MS.gene99599:CDS	65.0%
!!	CGGCGCCGGTAGGAAGGAAG+AGG	+	chr5.4:45406570-45406589	MS.gene99599:CDS	70.0%
!!	GAAGACCGGCGCCGGTAGGA+AGG	+	chr5.4:45406564-45406583	MS.gene99599:CDS	70.0%
!!	TGGAGAAGACCGGCGCCGGT+AGG	+	chr5.4:45406560-45406579	MS.gene99599:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	45406556	45406921	45406556	ID=MS.gene99599
chr5.4	mRNA	45406556	45406921	45406556	ID=MS.gene99599.t1;Parent=MS.gene99599
chr5.4	exon	45406556	45406921	45406556	ID=MS.gene99599.t1.exon1;Parent=MS.gene99599.t1
chr5.4	CDS	45406556	45406921	45406556	ID=cds.MS.gene99599.t1;Parent=MS.gene99599.t1

Gene Sequence	>MS.gene99599 ATGGTGGAGAAGACCGGCGCCGGTAGGAAGGAAGAGGTTGTCACCAGAGAGTACACCATCAACCTCCACAAACGCCTCCATGGCTGCACATTTAAGAAGAAAGCTCCTAAAGCAATAAAGGAGATCAGGAAGTTTGCCCAAAAGGCTATGGGAACTAATGATGTTAGAGTGTATGTCAAGTTGAACAAGGCTATTTGGAGCCAGGGGATCAGAAGTGTCCCAAGGAGGGTTAGGGTACGCATTGCCCGCAAGAGGAACGATGATGAGGATGCTAAGGAAGAGCTCTACTCACTTGTTACCGTTGTTGAAATCCCCAAGGAAGAACTCAAAGGCTTGGGCACAAAGGTTATTGATGATGAGGATTGA
Protein sequence	>MS.gene99599.t1 MVEKTGAGRKEEVVTREYTINLHKRLHGCTFKKKAPKAIKEIRKFAQKAMGTNDVRVYVKLNKAIWSQGIRSVPRRVRVRIARKRNDDEDAKEELYSLVTVVEIPKEELKGLGTKVIDDED