AlfalfaGEDB | MS.gene74367

M. sativa cultivar XinJiangDaYe / MS.gene74367

KEGG: -
GO: GO:0006950 GO:0006952 GO:0008150 GO:0050896
PFAM: PF00407.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74367.t1	XP_003628192.1	92.3	155	11	1	1	155	1	154	1.50E-79	305.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74367.t1	Q9SSK5	37.3	150	91	3	3	150	7	155	2.4e-22	106.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74367.t1	G7L7S7	92.3	155	11	1	1	155	1	154	1.1e-79	305.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene74367	0.802108	6.56E-49	-1.69E-46
MS.gene049254	MS.gene74367	0.802447	5.59E-49	-1.69E-46
MS.gene049272	MS.gene74367	0.810427	1.16E-50	-1.69E-46
MS.gene049404	MS.gene74367	0.805181	1.51E-49	-1.69E-46
MS.gene049435	MS.gene74367	0.831345	1.76E-55	-1.69E-46
MS.gene049501	MS.gene74367	0.825204	5.32E-54	-1.69E-46
MS.gene049850	MS.gene74367	0.829644	4.59E-55	-1.69E-46
MS.gene050021	MS.gene74367	0.817462	3.26E-52	-1.69E-46
MS.gene050026	MS.gene74367	0.813803	2.13E-51	-1.69E-46
MS.gene050137	MS.gene74367	0.845377	4.22E-59	-1.69E-46
MS.gene050156	MS.gene74367	0.840002	1.13E-57	-1.69E-46
MS.gene05016	MS.gene74367	0.823031	1.72E-53	-1.69E-46
MS.gene050201	MS.gene74367	0.801597	8.35E-49	-1.69E-46
MS.gene050226	MS.gene74367	0.80984	1.55E-50	-1.69E-46
MS.gene050227	MS.gene74367	0.81271	3.70E-51	-1.69E-46
MS.gene050228	MS.gene74367	0.812247	4.67E-51	-1.69E-46
MS.gene050355	MS.gene74367	0.807034	6.16E-50	-1.69E-46
MS.gene050442	MS.gene74367	0.822833	1.92E-53	-1.69E-46
MS.gene050494	MS.gene74367	0.809786	1.59E-50	-1.69E-46
MS.gene050561	MS.gene74367	0.80006	1.72E-48	-1.69E-46
MS.gene050680	MS.gene74367	0.829538	4.87E-55	-1.69E-46
MS.gene050750	MS.gene74367	0.812714	3.69E-51	-1.69E-46
MS.gene050780	MS.gene74367	0.814344	1.62E-51	-1.69E-46
MS.gene050787	MS.gene74367	0.833981	3.90E-56	-1.69E-46
MS.gene050807	MS.gene74367	0.824148	9.44E-54	-1.69E-46
MS.gene050808	MS.gene74367	0.800611	1.33E-48	-1.69E-46
MS.gene050820	MS.gene74367	0.802564	5.28E-49	-1.69E-46
MS.gene050826	MS.gene74367	0.810135	1.34E-50	-1.69E-46
MS.gene050844	MS.gene74367	0.831335	1.77E-55	-1.69E-46
MS.gene050953	MS.gene74367	0.803888	2.81E-49	-1.69E-46
MS.gene050959	MS.gene74367	0.800833	1.20E-48	-1.69E-46
MS.gene051190	MS.gene74367	0.811988	5.31E-51	-1.69E-46
MS.gene051247	MS.gene74367	0.824845	6.47E-54	-1.69E-46
MS.gene05125	MS.gene74367	0.804345	2.26E-49	-1.69E-46
MS.gene051265	MS.gene74367	0.850007	2.24E-60	-1.69E-46
MS.gene051279	MS.gene74367	0.810522	1.11E-50	-1.69E-46
MS.gene05132	MS.gene74367	0.810193	1.30E-50	-1.69E-46
MS.gene051594	MS.gene74367	0.805874	1.08E-49	-1.69E-46
MS.gene051719	MS.gene74367	0.83697	6.85E-57	-1.69E-46
MS.gene051720	MS.gene74367	0.830376	3.04E-55	-1.69E-46
MS.gene051744	MS.gene74367	0.809874	1.52E-50	-1.69E-46
MS.gene051800	MS.gene74367	0.825345	4.93E-54	-1.69E-46
MS.gene051957	MS.gene74367	0.805535	1.27E-49	-1.69E-46
MS.gene052020	MS.gene74367	0.815988	6.97E-52	-1.69E-46
MS.gene052190	MS.gene74367	0.845106	4.99E-59	-1.69E-46
MS.gene052323	MS.gene74367	0.804428	2.17E-49	-1.69E-46
MS.gene052333	MS.gene74367	0.847362	1.21E-59	-1.69E-46
MS.gene052488	MS.gene74367	0.8297	4.44E-55	-1.69E-46
MS.gene052516	MS.gene74367	0.805992	1.02E-49	-1.69E-46
MS.gene052519	MS.gene74367	0.821878	3.19E-53	-1.69E-46
MS.gene052582	MS.gene74367	0.816015	6.87E-52	-1.69E-46
MS.gene052584	MS.gene74367	0.81908	1.40E-52	-1.69E-46
MS.gene052611	MS.gene74367	0.822195	2.70E-53	-1.69E-46
MS.gene052613	MS.gene74367	0.812948	3.28E-51	-1.69E-46
MS.gene052773	MS.gene74367	0.820017	8.57E-53	-1.69E-46
MS.gene052829	MS.gene74367	0.809775	1.60E-50	-1.69E-46
MS.gene052843	MS.gene74367	0.800923	1.15E-48	-1.69E-46
MS.gene052863	MS.gene74367	0.821974	3.03E-53	-1.69E-46
MS.gene052867	MS.gene74367	0.811937	5.45E-51	-1.69E-46
MS.gene052896	MS.gene74367	0.806168	9.38E-50	-1.69E-46
MS.gene053054	MS.gene74367	-0.807814	4.21E-50	-1.69E-46
MS.gene053147	MS.gene74367	0.830064	3.62E-55	-1.69E-46
MS.gene053166	MS.gene74367	0.858332	8.86E-63	-1.69E-46
MS.gene053201	MS.gene74367	0.822033	2.94E-53	-1.69E-46
MS.gene053221	MS.gene74367	0.825386	4.82E-54	-1.69E-46
MS.gene053226	MS.gene74367	0.826403	2.77E-54	-1.69E-46
MS.gene053331	MS.gene74367	0.815503	8.94E-52	-1.69E-46
MS.gene053584	MS.gene74367	0.80148	8.83E-49	-1.69E-46
MS.gene053647	MS.gene74367	0.805365	1.38E-49	-1.69E-46
MS.gene053966	MS.gene74367	0.825958	3.53E-54	-1.69E-46
MS.gene053974	MS.gene74367	0.809362	1.96E-50	-1.69E-46
MS.gene053998	MS.gene74367	0.823926	1.06E-53	-1.69E-46
MS.gene05414	MS.gene74367	0.822996	1.76E-53	-1.69E-46
MS.gene05415	MS.gene74367	0.839238	1.79E-57	-1.69E-46
MS.gene054236	MS.gene74367	0.822895	1.85E-53	-1.69E-46
MS.gene054291	MS.gene74367	-0.847166	1.37E-59	-1.69E-46
MS.gene054467	MS.gene74367	0.871017	9.41E-67	-1.69E-46
MS.gene054468	MS.gene74367	0.864462	1.20E-64	-1.69E-46
MS.gene054472	MS.gene74367	0.803158	3.98E-49	-1.69E-46
MS.gene054559	MS.gene74367	0.835921	1.27E-56	-1.69E-46
MS.gene054581	MS.gene74367	0.823287	1.50E-53	-1.69E-46
MS.gene054651	MS.gene74367	0.807516	4.86E-50	-1.69E-46
MS.gene05468	MS.gene74367	0.820974	5.16E-53	-1.69E-46
MS.gene054775	MS.gene74367	0.812478	4.15E-51	-1.69E-46
MS.gene055227	MS.gene74367	0.835709	1.43E-56	-1.69E-46
MS.gene055228	MS.gene74367	0.805222	1.48E-49	-1.69E-46
MS.gene055356	MS.gene74367	0.869326	3.37E-66	-1.69E-46
MS.gene055357	MS.gene74367	0.853257	2.69E-61	-1.69E-46
MS.gene055358	MS.gene74367	0.869548	2.85E-66	-1.69E-46
MS.gene055466	MS.gene74367	0.811427	7.04E-51	-1.69E-46
MS.gene055507	MS.gene74367	0.80317	3.96E-49	-1.69E-46
MS.gene055804	MS.gene74367	0.804332	2.27E-49	-1.69E-46
MS.gene055818	MS.gene74367	0.849034	4.19E-60	-1.69E-46
MS.gene055926	MS.gene74367	0.823704	1.20E-53	-1.69E-46
MS.gene05604	MS.gene74367	0.863795	1.93E-64	-1.69E-46
MS.gene056050	MS.gene74367	0.816525	5.29E-52	-1.69E-46
MS.gene056173	MS.gene74367	0.841894	3.60E-58	-1.69E-46
MS.gene056174	MS.gene74367	0.803506	3.37E-49	-1.69E-46
MS.gene056366	MS.gene74367	0.808957	2.40E-50	-1.69E-46
MS.gene056506	MS.gene74367	0.818193	2.23E-52	-1.69E-46
MS.gene056542	MS.gene74367	0.80136	9.34E-49	-1.69E-46
MS.gene056543	MS.gene74367	0.800024	1.75E-48	-1.69E-46
MS.gene056545	MS.gene74367	0.804751	1.86E-49	-1.69E-46
MS.gene056553	MS.gene74367	0.825179	5.40E-54	-1.69E-46
MS.gene056595	MS.gene74367	0.802996	4.30E-49	-1.69E-46
MS.gene057012	MS.gene74367	0.803021	4.25E-49	-1.69E-46
MS.gene057026	MS.gene74367	0.84945	3.21E-60	-1.69E-46
MS.gene057060	MS.gene74367	0.833675	4.65E-56	-1.69E-46
MS.gene057314	MS.gene74367	0.809391	1.94E-50	-1.69E-46
MS.gene057444	MS.gene74367	0.830889	2.28E-55	-1.69E-46
MS.gene057693	MS.gene74367	0.807378	5.21E-50	-1.69E-46
MS.gene057869	MS.gene74367	0.811677	6.21E-51	-1.69E-46
MS.gene057915	MS.gene74367	0.834902	2.29E-56	-1.69E-46
MS.gene058010	MS.gene74367	0.808166	3.54E-50	-1.69E-46
MS.gene058037	MS.gene74367	0.80296	4.38E-49	-1.69E-46
MS.gene058294	MS.gene74367	0.80467	1.93E-49	-1.69E-46
MS.gene058625	MS.gene74367	0.844953	5.49E-59	-1.69E-46
MS.gene058636	MS.gene74367	0.800014	1.76E-48	-1.69E-46
MS.gene058829	MS.gene74367	0.847852	8.90E-60	-1.69E-46
MS.gene058979	MS.gene74367	0.831574	1.55E-55	-1.69E-46
MS.gene059015	MS.gene74367	0.801373	9.29E-49	-1.69E-46
MS.gene059128	MS.gene74367	0.8273	1.69E-54	-1.69E-46
MS.gene059161	MS.gene74367	0.810446	1.15E-50	-1.69E-46
MS.gene059272	MS.gene74367	0.832774	7.80E-56	-1.69E-46
MS.gene059439	MS.gene74367	0.808714	2.70E-50	-1.69E-46
MS.gene059584	MS.gene74367	0.818872	1.56E-52	-1.69E-46
MS.gene059607	MS.gene74367	0.837481	5.07E-57	-1.69E-46
MS.gene060137	MS.gene74367	-0.81829	2.12E-52	-1.69E-46
MS.gene060167	MS.gene74367	0.812365	4.40E-51	-1.69E-46
MS.gene060312	MS.gene74367	0.835411	1.70E-56	-1.69E-46
MS.gene060322	MS.gene74367	0.857422	1.65E-62	-1.69E-46
MS.gene06052	MS.gene74367	0.811187	7.94E-51	-1.69E-46
MS.gene060553	MS.gene74367	0.80404	2.61E-49	-1.69E-46
MS.gene060572	MS.gene74367	0.809044	2.30E-50	-1.69E-46
MS.gene060781	MS.gene74367	0.849267	3.61E-60	-1.69E-46
MS.gene060803	MS.gene74367	0.8072	5.68E-50	-1.69E-46
MS.gene060998	MS.gene74367	0.807095	5.98E-50	-1.69E-46
MS.gene061040	MS.gene74367	-0.801023	1.09E-48	-1.69E-46
MS.gene061114	MS.gene74367	-0.813749	2.19E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene77763	MS.gene74367	PPI
MS.gene016315	MS.gene74367	PPI
MS.gene016326	MS.gene74367	PPI
MS.gene77769	MS.gene74367	PPI
MS.gene016337	MS.gene74367	PPI
MS.gene83129	MS.gene74367	PPI
MS.gene74364	MS.gene74367	PPI
MS.gene016327	MS.gene74367	PPI
MS.gene74352	MS.gene74367	PPI
MS.gene59140	MS.gene74367	PPI
MS.gene59869	MS.gene74367	PPI
MS.gene59862	MS.gene74367	PPI
MS.gene016336	MS.gene74367	PPI
MS.gene74354	MS.gene74367	PPI
MS.gene74367	MS.gene59859	PPI
MS.gene74344	MS.gene74367	PPI
MS.gene99548	MS.gene74367	PPI
MS.gene74367	MS.gene016307	PPI
MS.gene066940	MS.gene74367	PPI
MS.gene74367	MS.gene59840	PPI
MS.gene74367	MS.gene058386	PPI
MS.gene74341	MS.gene74367	PPI
MS.gene62988	MS.gene74367	PPI
MS.gene74353	MS.gene74367	PPI
MS.gene77771	MS.gene74367	PPI
MS.gene016314	MS.gene74367	PPI
MS.gene74367	MS.gene74341	PPI
MS.gene77780	MS.gene74367	PPI
MS.gene016330	MS.gene74367	PPI
MS.gene77758	MS.gene74367	PPI
MS.gene74346	MS.gene74367	PPI
MS.gene59860	MS.gene74367	PPI
MS.gene74367	MS.gene016315	PPI
MS.gene026605	MS.gene74367	PPI
MS.gene058383	MS.gene74367	PPI
MS.gene77770	MS.gene74367	PPI
MS.gene74367	MS.gene016317	PPI
MS.gene058386	MS.gene74367	PPI
MS.gene74343	MS.gene74367	PPI
MS.gene77774	MS.gene74367	PPI
MS.gene058382	MS.gene74367	PPI
MS.gene74367	MS.gene74346	PPI
MS.gene74365	MS.gene74367	PPI
MS.gene74367	MS.gene77781	PPI
MS.gene77768	MS.gene74367	PPI
MS.gene59857	MS.gene74367	PPI
MS.gene59863	MS.gene74367	PPI
MS.gene59841	MS.gene74367	PPI
MS.gene74342	MS.gene74367	PPI
MS.gene016307	MS.gene74367	PPI
MS.gene59849	MS.gene74367	PPI
MS.gene058384	MS.gene74367	PPI
MS.gene74367	MS.gene59847	PPI
MS.gene74355	MS.gene74367	PPI
MS.gene74358	MS.gene74367	PPI
MS.gene74367	MS.gene59860	PPI
MS.gene77761	MS.gene74367	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74367.t1	MTR_8g045400	92.258	155	11	1	1	155	1	154	4.62e-104	294
MS.gene74367.t1	MTR_8g045490	79.605	152	31	0	1	152	1	152	2.65e-91	262
MS.gene74367.t1	MTR_8g045520	80.645	155	30	0	1	155	1	155	3.42e-91	262
MS.gene74367.t1	MTR_8g045695	76.129	155	36	1	1	155	1	154	2.23e-86	249
MS.gene74367.t1	MTR_8g045735	72.258	155	42	1	1	155	1	154	1.59e-82	240
MS.gene74367.t1	MTR_8g045640	74.839	155	38	1	1	155	1	154	3.35e-82	239
MS.gene74367.t1	MTR_8g045570	71.711	152	43	0	1	152	1	152	3.52e-82	239
MS.gene74367.t1	MTR_8g045665	73.548	155	40	1	1	155	1	154	4.21e-82	239
MS.gene74367.t1	MTR_8g045560	73.548	155	40	1	1	155	1	154	2.38e-81	237
MS.gene74367.t1	MTR_8g045300	69.677	155	47	0	1	155	1	155	3.00e-80	234
MS.gene74367.t1	MTR_8g045555	70.395	152	45	0	1	152	1	152	1.95e-79	232
MS.gene74367.t1	MTR_1g030820	37.908	153	92	2	1	151	1	152	1.94e-38	128
MS.gene74367.t1	MTR_1g030810	39.216	153	90	2	1	151	1	152	5.75e-38	127
MS.gene74367.t1	MTR_1g030840	37.580	157	88	5	1	149	1	155	1.20e-29	106
MS.gene74367.t1	MTR_8g012550	34.483	145	92	3	6	149	8	150	4.20e-27	99.8
MS.gene74367.t1	MTR_1g031640	37.838	148	83	5	7	152	7	147	8.84e-25	93.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74367.t1	AT5G28010	36.000	150	93	3	3	150	15	163	2.31e-28	103
MS.gene74367.t1	AT5G28010	36.000	150	93	3	3	150	15	163	2.31e-28	103
MS.gene74367.t1	AT1G70890	38.000	150	90	3	3	150	7	155	5.34e-27	99.4
MS.gene74367.t1	AT1G70840	36.000	150	93	3	3	150	20	168	1.37e-26	98.6
MS.gene74367.t1	AT1G70830	37.333	150	91	3	3	150	22	170	3.34e-26	97.8
MS.gene74367.t1	AT1G70830	37.333	150	91	3	3	150	22	170	6.54e-26	97.8
MS.gene74367.t1	AT1G70830	37.333	150	91	3	3	150	22	170	2.46e-25	97.4
MS.gene74367.t1	AT5G28000	34.667	150	95	3	3	150	13	161	6.06e-25	94.4
MS.gene74367.t1	AT1G70830	37.333	150	91	3	3	150	22	170	8.45e-25	97.8
MS.gene74367.t1	AT1G70830	36.000	150	93	3	3	150	184	332	2.76e-23	93.6
MS.gene74367.t1	AT1G70850	35.333	150	94	3	3	150	9	157	6.86e-24	93.6
MS.gene74367.t1	AT1G70830	36.000	150	93	3	3	150	137	285	7.37e-24	94.4
MS.gene74367.t1	AT1G70830	33.333	93	61	1	59	150	31	123	8.78e-13	64.7
MS.gene74367.t1	AT1G70850	35.333	150	94	3	3	150	9	157	2.27e-23	93.6
MS.gene74367.t1	AT1G70850	36.429	140	86	3	13	150	175	313	3.53e-22	90.5
MS.gene74367.t1	AT1G70850	35.333	150	94	3	3	150	9	157	2.27e-23	93.6
MS.gene74367.t1	AT1G70850	36.429	140	86	3	13	150	175	313	3.53e-22	90.5
MS.gene74367.t1	AT1G23130	30.201	149	102	2	3	150	10	157	1.80e-21	85.1
MS.gene74367.t1	AT4G23670	33.333	153	99	3	1	152	1	151	2.93e-20	82.0
MS.gene74367.t1	AT1G23120	28.000	150	103	3	1	150	1	145	3.38e-20	81.6
MS.gene74367.t1	AT1G23120	28.472	144	98	3	7	150	57	195	9.94e-20	81.6
MS.gene74367.t1	AT1G70870	34.437	151	86	4	1	151	1	138	1.06e-19	80.1
MS.gene74367.t1	AT1G70880	30.000	150	102	3	3	150	8	156	5.79e-19	79.0
MS.gene74367.t1	AT2G01520	30.065	153	104	3	1	152	1	151	3.12e-18	76.6
MS.gene74367.t1	AT1G14930	28.387	155	108	3	1	154	1	153	4.24e-18	76.3
MS.gene74367.t1	AT1G35260	29.452	146	99	3	7	150	6	149	1.46e-17	75.1
MS.gene74367.t1	AT2G01530	30.719	153	103	3	1	152	1	151	3.14e-17	73.9
MS.gene74367.t1	AT4G14060	30.065	153	104	3	1	152	1	151	3.64e-17	73.9
MS.gene74367.t1	AT4G23680	28.947	152	105	3	1	151	1	150	1.80e-16	72.0
MS.gene74367.t1	AT1G30990	30.464	151	101	4	1	150	1	148	2.29e-15	69.3
MS.gene74367.t1	AT1G14940	27.742	155	109	3	1	154	1	153	2.74e-15	68.9
MS.gene74367.t1	AT1G14950	28.387	155	108	3	1	154	1	153	4.97e-15	68.6
MS.gene74367.t1	AT1G24020	30.323	155	106	2	1	154	1	154	1.49e-14	67.0
MS.gene74367.t1	AT1G24020	30.323	155	106	2	1	154	1	154	1.49e-14	67.0
MS.gene74367.t1	AT1G35310	25.899	139	100	3	13	150	12	148	4.27e-14	65.9
MS.gene74367.t1	AT3G26450	28.571	154	106	4	1	152	1	152	8.84e-14	65.1
MS.gene74367.t1	AT1G14960	28.289	152	106	3	1	151	1	150	1.26e-12	62.0
MS.gene74367.t1	AT3G26460	30.128	156	101	5	1	152	1	152	2.04e-12	61.6

Find 28 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCTTCACCTTCATGAAGTT+TGG	0.292428	8.4:-55928669	None:intergenic
AATGGCTGGATGGACTTATT+AGG	0.352875	8.4:+55929704	MS.gene74367:CDS
AATTCAGAAATGGTGCTTGC+TGG	0.366885	8.4:+55928539	None:intergenic
AAAATAACTTTCAAGCTCTT+CGG	0.428417	8.4:+55929554	MS.gene74367:CDS
ATCATTGAAGTAATTGATAA+TGG	0.436398	8.4:+55929614	MS.gene74367:CDS
ATGGCAGTGGAGCTGCAAAA+TGG	0.452179	8.4:+55929639	MS.gene74367:CDS
AATAAGTCCATCCAGCCATT+TGG	0.456616	8.4:-55929701	None:intergenic
AAACACTGGACTTATGTCAT+AGG	0.490184	8.4:+55928713	MS.gene74367:CDS
AACTTCATGAAGGTGAAGAT+TGG	0.494334	8.4:+55928672	MS.gene74367:CDS
AGACATTGATGAGCACTATA+AGG	0.495099	8.4:+55929580	MS.gene74367:CDS
ATATTGACCCACCAAATGGC+TGG	0.520216	8.4:+55929690	MS.gene74367:CDS
GAGGATATTGACCCACCAAA+TGG	0.531703	8.4:+55929686	MS.gene74367:CDS
GGTAAACTTAGTACTGAACT+TGG	0.538495	8.4:+55928560	MS.gene74367:CDS
ATAACTTTCAAGCTCTTCGG+TGG	0.552870	8.4:+55929557	MS.gene74367:CDS
AAGTCCATCCAGCCATTTGG+TGG	0.553291	8.4:-55929698	None:intergenic
ATTGATAATGGTGATGGCAG+TGG	0.556824	8.4:+55929626	MS.gene74367:CDS
TGACCCACCAAATGGCTGGA+TGG	0.567137	8.4:+55929694	MS.gene74367:CDS
ATTGATCTTCTCATATTCAA+CGG	0.576809	8.4:-55929663	None:intergenic
GTTGATGCTCATCTTGTCAA+AGG	0.591021	8.4:+55929743	MS.gene74367:CDS
CATCATGCCAAACTTCATGA+AGG	0.593864	8.4:+55928662	MS.gene74367:CDS
TGACATCACAATAGATGGCA+AGG	0.606404	8.4:+55929484	MS.gene74367:intron
AGTTTGAAGAACTTGTCAGC+GGG	0.609187	8.4:-55928593	None:intergenic
GTAAACTTAGTACTGAACTT+GGG	0.614711	8.4:+55928561	MS.gene74367:CDS
GAAGTAATTGATAATGGTGA+TGG	0.618480	8.4:+55929620	MS.gene74367:CDS
ACACTGATTCAGTTAAACAC+TGG	0.620261	8.4:+55928699	MS.gene74367:CDS
AGTCCATCCAGCCATTTGGT+GGG	0.658787	8.4:-55929697	None:intergenic
TAGTTTGAAGAACTTGTCAG+CGG	0.665980	8.4:-55928594	None:intergenic
TGAATATGAGAAGATCAATG+AGG	0.699788	8.4:+55929667	MS.gene74367:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATACATTGTTTTAATTTAAT+TGG	+	chr8.4:55929448-55929467	MS.gene74367:intron	10.0%
!!	ACAATATATAGAATAAGTGA+TGG	+	chr8.4:55929075-55929094	MS.gene74367:intron	20.0%
!!	ATCATTGAAGTAATTGATAA+TGG	+	chr8.4:55929614-55929633	MS.gene74367:CDS	20.0%
!!	CATAATCTTCGTATAAAATA+TGG	-	chr8.4:55928863-55928882	None:intergenic	20.0%
!!	GATTTACATGTCTAAATAAA+TGG	+	chr8.4:55929128-55929147	MS.gene74367:intron	20.0%
!!	GTAGAGTTATGAAAAATATA+TGG	-	chr8.4:55929428-55929447	None:intergenic	20.0%
!!	TAAAATGCTAAATACTCATT+AGG	+	chr8.4:55929196-55929215	MS.gene74367:intron	20.0%
!!	TGCTCTTATTAATTGAATTA+AGG	-	chr8.4:55928834-55928853	None:intergenic	20.0%
!!!	AATTCAATTAATAAGAGCAT+AGG	+	chr8.4:55928835-55928854	MS.gene74367:intron	20.0%
!!!	ATTCAATTAATAAGAGCATA+GGG	+	chr8.4:55928836-55928855	MS.gene74367:intron	20.0%
!	AAAATAACTTTCAAGCTCTT+CGG	+	chr8.4:55929554-55929573	MS.gene74367:CDS	25.0%
!	AAATAAACAAGATGATGACT+TGG	-	chr8.4:55928771-55928790	None:intergenic	25.0%
!	ACTTTATCTTTGTCATCTTT+AGG	-	chr8.4:55928887-55928906	None:intergenic	25.0%
!	ATTGATCTTCTCATATTCAA+CGG	-	chr8.4:55929666-55929685	None:intergenic	25.0%
!!	TATTGTCTCATTGATTGATT+TGG	-	chr8.4:55929061-55929080	None:intergenic	25.0%
	AAAAATAAGACACTGTTTCC+TGG	+	chr8.4:55928933-55928952	MS.gene74367:intron	30.0%
	ACAAATTCATATATGATGCC+AGG	-	chr8.4:55928954-55928973	None:intergenic	30.0%
	ATAGATACGTAGTACATACT+TGG	+	chr8.4:55929159-55929178	MS.gene74367:intron	30.0%
	CATATATGAATTTGTGCAGA+TGG	+	chr8.4:55928959-55928978	MS.gene74367:intron	30.0%
	TGAATATGAGAAGATCAATG+AGG	+	chr8.4:55929667-55929686	MS.gene74367:CDS	30.0%
!	GTAAACTTAGTACTGAACTT+GGG	+	chr8.4:55928561-55928580	MS.gene74367:CDS	30.0%
!!	GAAGTAATTGATAATGGTGA+TGG	+	chr8.4:55929620-55929639	MS.gene74367:CDS	30.0%
	AAACACTGGACTTATGTCAT+AGG	+	chr8.4:55928713-55928732	MS.gene74367:CDS	35.0%
	AACTTCATGAAGGTGAAGAT+TGG	+	chr8.4:55928672-55928691	MS.gene74367:CDS	35.0%
	AGACATTGATGAGCACTATA+AGG	+	chr8.4:55929580-55929599	MS.gene74367:CDS	35.0%
	ATATGAATTTGTGCAGATGG+AGG	+	chr8.4:55928962-55928981	MS.gene74367:intron	35.0%
	ATCTTCACCTTCATGAAGTT+TGG	-	chr8.4:55928672-55928691	None:intergenic	35.0%
	TAGTTTGAAGAACTTGTCAG+CGG	-	chr8.4:55928597-55928616	None:intergenic	35.0%
!	GGTAAACTTAGTACTGAACT+TGG	+	chr8.4:55928560-55928579	MS.gene74367:CDS	35.0%
!!	ACACTGATTCAGTTAAACAC+TGG	+	chr8.4:55928699-55928718	MS.gene74367:CDS	35.0%
	AATAAGTCCATCCAGCCATT+TGG	-	chr8.4:55929704-55929723	None:intergenic	40.0%
	AGTTTGAAGAACTTGTCAGC+GGG	-	chr8.4:55928596-55928615	None:intergenic	40.0%
	ATAACTTTCAAGCTCTTCGG+TGG	+	chr8.4:55929557-55929576	MS.gene74367:CDS	40.0%
	CATCATGCCAAACTTCATGA+AGG	+	chr8.4:55928662-55928681	MS.gene74367:CDS	40.0%
	GCCATCTATTGTGATGTCAT+GGG	-	chr8.4:55929483-55929502	None:intergenic	40.0%
	GTCCTAAACGATATGTGAGA+AGG	+	chr8.4:55929296-55929315	MS.gene74367:intron	40.0%
	GTTGATGCTCATCTTGTCAA+AGG	+	chr8.4:55929743-55929762	MS.gene74367:CDS	40.0%
	TCCCATGACATCACAATAGA+TGG	+	chr8.4:55929479-55929498	MS.gene74367:intron	40.0%
	TGACATCACAATAGATGGCA+AGG	+	chr8.4:55929484-55929503	MS.gene74367:intron	40.0%
	TGCCATCTATTGTGATGTCA+TGG	-	chr8.4:55929484-55929503	None:intergenic	40.0%
	TGCCTTCTCACATATCGTTT+AGG	-	chr8.4:55929301-55929320	None:intergenic	40.0%
!	AATGGCTGGATGGACTTATT+AGG	+	chr8.4:55929704-55929723	MS.gene74367:CDS	40.0%
!!	ATTGATAATGGTGATGGCAG+TGG	+	chr8.4:55929626-55929645	MS.gene74367:CDS	40.0%
	ATATTGACCCACCAAATGGC+TGG	+	chr8.4:55929690-55929709	MS.gene74367:CDS	45.0%
	GAGGATATTGACCCACCAAA+TGG	+	chr8.4:55929686-55929705	MS.gene74367:CDS	45.0%
	TTGTGCAGATGGAGGATCTA+TGG	+	chr8.4:55928970-55928989	MS.gene74367:intron	45.0%
	AAGTCCATCCAGCCATTTGG+TGG	-	chr8.4:55929701-55929720	None:intergenic	50.0%
	AGTCCATCCAGCCATTTGGT+GGG	-	chr8.4:55929700-55929719	None:intergenic	50.0%
	ATGGCAGTGGAGCTGCAAAA+TGG	+	chr8.4:55929639-55929658	MS.gene74367:CDS	50.0%
	CAGATGGAGGATCTATGGAG+AGG	+	chr8.4:55928975-55928994	MS.gene74367:intron	50.0%
	TGACCCACCAAATGGCTGGA+TGG	+	chr8.4:55929694-55929713	MS.gene74367:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	55928548	55929778	55928548	ID=MS.gene74367
chr8.4	mRNA	55928548	55929778	55928548	ID=MS.gene74367.t1;Parent=MS.gene74367
chr8.4	exon	55928548	55928734	55928548	ID=MS.gene74367.t1.exon1;Parent=MS.gene74367.t1
chr8.4	CDS	55928548	55928734	55928548	ID=cds.MS.gene74367.t1;Parent=MS.gene74367.t1
chr8.4	exon	55929498	55929778	55929498	ID=MS.gene74367.t1.exon2;Parent=MS.gene74367.t1
chr8.4	CDS	55929498	55929778	55929498	ID=cds.MS.gene74367.t1;Parent=MS.gene74367.t1

Gene Sequence

>MS.gene74367

ATGGTGCTTGCTGGTAAACTTAGTACTGAACTTGGGATCAAAACACCCGCTGACAAGTTCTTCAAACTATATGCATCAGAACTTCACGAAGTGCAAAACATTAGTGAAAGAATTCATCATGCCAAACTTCATGAAGGTGAAGATTGGCATCACACTGATTCAGTTAAACACTGGACTTATGTCATAGGTAATTAAGTTTCTAATTCATTTGACTTCATAACCAAGTCATCATCTTGTTTATTTATATATTTTTTTTGAACAATTTGTTTATTTATATATTGATCCTTAATTCAATTAATAAGAGCATAGGGACCATATTTTATACGAAGATTATGTCCTAAAGATGACAAAGATAAAGTTTTTCAGCTGTGAAATTTAGATACAAAAAAATAAGACACTGTTTCCTGGCATCATATATGAATTTGTGCAGATGGAGGATCTATGGAGAGGAAAAGATAAAAAAAATAAAACAGTCAGAAAAAAAAAACAATTCGATCAAAGTTTAATTAACCAAATCAATCAATGAGACAATATATAGAATAAGTGATGGATTTATAGATACATCATAGTACATATACTTGATTTACATGTCTAAATAAATGGTAGTTACTATAGATACGTAGTACATACTTGGTATTCATGTCAAAATAAAATGCTAAATACTCATTAGGAGATACATGATTGACTTTATAGTTGTAATGAGTGCATATAATTCAGACTTCTTAAAGATATTCAAATATTGCATAGAGTCCTAAACGATATGTGAGAAGGCAACTAAACATTATATAGATTATTAATATTCATTTTTTTGTTTAAAAACTATTTTATTTGAAAAATGAAAACAATTTAATTGATTAGTTATTTTAAAAAAATGTGTCCATATATTTTTCATAACTCTACATACATTGTTTTAATTTAATTGGTATTAATTTCCCATGACATCACAATAGATGGCAAGGTAAACACATGTCATGAGAGTGTTGAAGAAGTTGATGAACAGAACAAAAAAATAACTTTCAAGCTCTTCGGTGGAGACATTGATGAGCACTATAAGGTCTTTAATCTGATCATTGAAGTAATTGATAATGGTGATGGCAGTGGAGCTGCAAAATGGACCGTTGAATATGAGAAGATCAATGAGGATATTGACCCACCAAATGGCTGGATGGACTTATTAGGCAAAATCACTCGAGAAGTTGATGCTCATCTTGTCAAAGGCACAAACATATAG

Protein sequence

>MS.gene74367.t1

MVLAGKLSTELGIKTPADKFFKLYASELHEVQNISERIHHAKLHEGEDWHHTDSVKHWTYVIDGKVNTCHESVEEVDEQNKKITFKLFGGDIDEHYKVFNLIIEVIDNGDGSGAAKWTVEYEKINEDIDPPNGWMDLLGKITREVDAHLVKGTNI