AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065904


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065904.t1 MTR_4g478130 77.987 159 5 1 1 129 1 159 1.11e-80 234
MS.gene065904.t1 MTR_4g478140 61.875 160 30 2 1 129 1 160 3.73e-60 182
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCACCTTCTGAAATCTTTC+AGG 0.221004 4.1:-27108601 None:intergenic
CTCTTTGGCACTCTTGCTAT+TGG 0.264799 4.1:+27108556 MS.gene065904:CDS
AGATCCCATGCTTCACTTGC+TGG 0.373899 4.1:-27108535 None:intergenic
CTACAACGTCTGCCAATTGC+TGG 0.406562 4.1:-27109714 None:intergenic
AAAGAGATCAAGAAGATGTT+TGG 0.416699 4.1:+27108481 None:intergenic
ACTTGTTTCCATCCAGCAAT+TGG 0.424661 4.1:+27109702 MS.gene065904:CDS
GATGGAAACAAGTGCGGCAT+TGG 0.436193 4.1:-27109692 None:intergenic
AGTGAAGCATGGGATCTCTT+TGG 0.449536 4.1:+27108541 MS.gene065904:CDS
TTGCATCCTTGTTTCTGTTG+AGG 0.456610 4.1:-27109753 None:intergenic
AAAGAAGTGGAGGTGGTTGA+AGG 0.462385 4.1:+27109550 MS.gene065904:CDS
AAGAAGTGGAGGTGGTTGAA+GGG 0.482356 4.1:+27109551 MS.gene065904:CDS
TTGGCAGACGTTGTAGAAGT+TGG 0.487317 4.1:+27109721 MS.gene065904:CDS
CACACTATTTAGAGTTCGTA+TGG 0.490157 4.1:+27109594 MS.gene065904:CDS
CGTATGGAAGTGATAGAGAA+AGG 0.493358 4.1:+27109610 MS.gene065904:CDS
CAGAAGGTGGAGCTAATTGA+AGG 0.500124 4.1:+27108613 MS.gene065904:CDS
AGAAGTGGAGGTGGTTGAAG+GGG 0.506956 4.1:+27109552 MS.gene065904:CDS
GTGGAGCTAATTGAAGGAGA+TGG 0.515580 4.1:+27108619 MS.gene065904:CDS
AGAGTATGAAGTCAAGGAAG+AGG 0.520181 4.1:+27109663 MS.gene065904:CDS
TGAAGTCAAGGAAGAGGAAG+CGG 0.526015 4.1:+27109669 MS.gene065904:CDS
TGGCACTCTTGCTATTGGAA+AGG 0.531710 4.1:+27108561 MS.gene065904:CDS
TACATTAGAGTATGAAGTCA+AGG 0.533592 4.1:+27109657 MS.gene065904:CDS
GAGCTAATTGAAGGAGATGG+AGG 0.540375 4.1:+27108622 MS.gene065904:CDS
GAAGTGGAGGTGGTTGAAGG+GGG 0.553306 4.1:+27109553 MS.gene065904:CDS
CGTGCCAGCAAGTGAAGCAT+GGG 0.555967 4.1:+27108531 MS.gene065904:CDS
TGAAAAGCACATCAAAGAAG+TGG 0.563701 4.1:+27109537 MS.gene065904:intron
ACGTGCCAGCAAGTGAAGCA+TGG 0.570168 4.1:+27108530 MS.gene065904:CDS
AACGTCTGCCAATTGCTGGA+TGG 0.574607 4.1:-27109710 None:intergenic
GCACATCAAAGAAGTGGAGG+TGG 0.578278 4.1:+27109543 MS.gene065904:intron
AGCTAATTGAAGGAGATGGA+GGG 0.580245 4.1:+27108623 MS.gene065904:CDS
GCTAATTGAAGGAGATGGAG+GGG 0.586950 4.1:+27108624 MS.gene065904:CDS
GCCTGAAAGATTTCAGAAGG+TGG 0.605828 4.1:+27108600 MS.gene065904:CDS
TGCTATTGGAAAGGTCGTTG+AGG 0.616559 4.1:+27108570 MS.gene065904:CDS
GATGCCTGAAAGATTTCAGA+AGG 0.633462 4.1:+27108597 MS.gene065904:CDS
AAAGCACATCAAAGAAGTGG+AGG 0.653324 4.1:+27109540 MS.gene065904:intron
TTGCTGGATGGAAACAAGTG+CGG 0.676967 4.1:-27109698 None:intergenic
GAATTACCTCAACAGAAACA+AGG 0.697908 4.1:+27109747 MS.gene065904:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATAATGAAAATAACC+TGG - chr4.1:27108679-27108698 None:intergenic 15.0%
!! ATTATTTACACTTGAATAAA+AGG - chr4.1:27109145-27109164 None:intergenic 15.0%
!!! TAACATAACATTAATTGTTT+TGG + chr4.1:27109450-27109469 MS.gene065904:intron 15.0%
!!! TATATTTTTGAGTATAATTG+TGG - chr4.1:27108724-27108743 None:intergenic 15.0%
!!! TTTTATTCAAGTGTAAATAA+TGG + chr4.1:27109144-27109163 MS.gene065904:intron 15.0%
!! AATTAAATAGAAAGTAGTCT+CGG - chr4.1:27108959-27108978 None:intergenic 20.0%
!! ATGATGAATTAACATACTTA+CGG + chr4.1:27109350-27109369 MS.gene065904:intron 20.0%
!! TTATCGTTACATAATTCATA+TGG + chr4.1:27108745-27108764 MS.gene065904:intron 20.0%
!!! ATAACATTAATTGTTTTGGT+CGG + chr4.1:27109454-27109473 MS.gene065904:intron 20.0%
! AATCTATAGATTCATGAGTT+TGG + chr4.1:27109307-27109326 MS.gene065904:intron 25.0%
! TGATGAATTAACATACTTAC+GGG + chr4.1:27109351-27109370 MS.gene065904:intron 25.0%
!! AACTTTTCTTTTAAACCAGT+TGG - chr4.1:27109502-27109521 None:intergenic 25.0%
!! ACTTTTCTTTTAAACCAGTT+GGG - chr4.1:27109501-27109520 None:intergenic 25.0%
!! CTATTGTATCATGAACATTT+TGG + chr4.1:27108996-27109015 MS.gene065904:intron 25.0%
ACATTTGATATGTAAGGATC+AGG - chr4.1:27108908-27108927 None:intergenic 30.0%
GACATCACATTTGATATGTA+AGG - chr4.1:27108914-27108933 None:intergenic 30.0%
TACATTAGAGTATGAAGTCA+AGG + chr4.1:27109657-27109676 MS.gene065904:CDS 30.0%
TTCATCTTAAGCTGAATAAG+TGG + chr4.1:27108866-27108885 MS.gene065904:intron 30.0%
! GTGCTTTTCATTATCAATCT+TGG - chr4.1:27109527-27109546 None:intergenic 30.0%
!! ATTGATTTTGCCATCCAAAA+AGG + chr4.1:27109091-27109110 MS.gene065904:intron 30.0%
AAATGTGATGTCCCTAACAA+TGG + chr4.1:27108922-27108941 MS.gene065904:intron 35.0%
ACACTTGAATAAAAGGCAAG+CGG - chr4.1:27109138-27109157 None:intergenic 35.0%
ATTATGTCTATGTACAGGTG+GGG + chr4.1:27109268-27109287 MS.gene065904:intron 35.0%
CATTATGTCTATGTACAGGT+GGG + chr4.1:27109267-27109286 MS.gene065904:intron 35.0%
GAATTACCTCAACAGAAACA+AGG + chr4.1:27109747-27109766 MS.gene065904:CDS 35.0%
GAGGATGTGTTTACAATTGA+TGG - chr4.1:27108792-27108811 None:intergenic 35.0%
! CACACTATTTAGAGTTCGTA+TGG + chr4.1:27109594-27109613 MS.gene065904:CDS 35.0%
! TGAAAAGCACATCAAAGAAG+TGG + chr4.1:27109537-27109556 MS.gene065904:intron 35.0%
AAAGCACATCAAAGAAGTGG+AGG + chr4.1:27109540-27109559 MS.gene065904:intron 40.0%
AACACAACATTCATGGACCT+AGG - chr4.1:27109064-27109083 None:intergenic 40.0%
AATAGAAAGTAGTCTCGGCA+AGG - chr4.1:27108954-27108973 None:intergenic 40.0%
ACTTGTTTCCATCCAGCAAT+TGG + chr4.1:27109702-27109721 MS.gene065904:CDS 40.0%
AGAGTATGAAGTCAAGGAAG+AGG + chr4.1:27109663-27109682 MS.gene065904:CDS 40.0%
AGCTAATTGAAGGAGATGGA+GGG + chr4.1:27108623-27108642 MS.gene065904:CDS 40.0%
ATAGCAATGTCATGTCTCCT+AGG + chr4.1:27109044-27109063 MS.gene065904:intron 40.0%
CACTTGAATAAAAGGCAAGC+GGG - chr4.1:27109137-27109156 None:intergenic 40.0%
CATCGTGAACACAACATTCA+TGG - chr4.1:27109071-27109090 None:intergenic 40.0%
CCACCATTATGTCTATGTAC+AGG + chr4.1:27109263-27109282 MS.gene065904:intron 40.0%
CCACCTGTACATAGACATAA+TGG - chr4.1:27109269-27109288 None:intergenic 40.0%
CCATTATGTCTATGTACAGG+TGG + chr4.1:27109266-27109285 MS.gene065904:intron 40.0%
CCTGTACATAGACATAATGG+TGG - chr4.1:27109266-27109285 None:intergenic 40.0%
CGTATGGAAGTGATAGAGAA+AGG + chr4.1:27109610-27109629 MS.gene065904:CDS 40.0%
GATGCCTGAAAGATTTCAGA+AGG + chr4.1:27108597-27108616 MS.gene065904:CDS 40.0%
TAACCACCTTATTCTCCACT+AGG - chr4.1:27108829-27108848 None:intergenic 40.0%
TTATGTCTATGTACAGGTGG+GGG + chr4.1:27109269-27109288 MS.gene065904:intron 40.0%
!! TCCACCTTCTGAAATCTTTC+AGG - chr4.1:27108604-27108623 None:intergenic 40.0%
!! TTGCATCCTTGTTTCTGTTG+AGG - chr4.1:27109756-27109775 None:intergenic 40.0%
AAAGAAGTGGAGGTGGTTGA+AGG + chr4.1:27109550-27109569 MS.gene065904:CDS 45.0%
AAGAAGTGGAGGTGGTTGAA+GGG + chr4.1:27109551-27109570 MS.gene065904:CDS 45.0%
AGTGAAGCATGGGATCTCTT+TGG + chr4.1:27108541-27108560 MS.gene065904:CDS 45.0%
CAAGGATCGCTCCATTGTTA+GGG - chr4.1:27108936-27108955 None:intergenic 45.0%
CAGAAGGTGGAGCTAATTGA+AGG + chr4.1:27108613-27108632 MS.gene065904:CDS 45.0%
CGTTGACCTAGTGGAGAATA+AGG + chr4.1:27108820-27108839 MS.gene065904:intron 45.0%
CTCAAACTCACATTCACTCC+AGG + chr4.1:27108658-27108677 MS.gene065904:intron 45.0%
GAGCTAATTGAAGGAGATGG+AGG + chr4.1:27108622-27108641 MS.gene065904:CDS 45.0%
GCCTGAAAGATTTCAGAAGG+TGG + chr4.1:27108600-27108619 MS.gene065904:CDS 45.0%
GCTAATTGAAGGAGATGGAG+GGG + chr4.1:27108624-27108643 MS.gene065904:CDS 45.0%
GGAGTTTGATCAGGTTTACC+TGG + chr4.1:27109475-27109494 MS.gene065904:intron 45.0%
GTGGAGCTAATTGAAGGAGA+TGG + chr4.1:27108619-27108638 MS.gene065904:CDS 45.0%
TGAAGTCAAGGAAGAGGAAG+CGG + chr4.1:27109669-27109688 MS.gene065904:CDS 45.0%
TGACCTAGTGGAGAATAAGG+TGG + chr4.1:27108823-27108842 MS.gene065904:intron 45.0%
TGCCATCCAAAAAGGTCTAG+CGG + chr4.1:27109099-27109118 MS.gene065904:intron 45.0%
TGCTATTGGAAAGGTCGTTG+AGG + chr4.1:27108570-27108589 MS.gene065904:CDS 45.0%
TGTAAACACATCCTCCGTTG+AGG + chr4.1:27108797-27108816 MS.gene065904:intron 45.0%
TTGCTGGATGGAAACAAGTG+CGG - chr4.1:27109701-27109720 None:intergenic 45.0%
TTGGCAGACGTTGTAGAAGT+TGG + chr4.1:27109721-27109740 MS.gene065904:CDS 45.0%
! CTCTTTGGCACTCTTGCTAT+TGG + chr4.1:27108556-27108575 MS.gene065904:CDS 45.0%
! TCTTTTAAACCAGTTGGGCC+AGG - chr4.1:27109496-27109515 None:intergenic 45.0%
!! GTTTTGGTCGGAGTTTGATC+AGG + chr4.1:27109466-27109485 MS.gene065904:intron 45.0%
!! TGGCACTCTTGCTATTGGAA+AGG + chr4.1:27108561-27108580 MS.gene065904:CDS 45.0%
AACGTCTGCCAATTGCTGGA+TGG - chr4.1:27109713-27109732 None:intergenic 50.0%
AGAAGTGGAGGTGGTTGAAG+GGG + chr4.1:27109552-27109571 MS.gene065904:CDS 50.0%
AGATCCCATGCTTCACTTGC+TGG - chr4.1:27108538-27108557 None:intergenic 50.0%
ATTGAAGGAGATGGAGGGGT+TGG + chr4.1:27108628-27108647 MS.gene065904:intron 50.0%
CTACAACGTCTGCCAATTGC+TGG - chr4.1:27109717-27109736 None:intergenic 50.0%
CTATGTACAGGTGGGGGATT+CGG + chr4.1:27109275-27109294 MS.gene065904:intron 50.0%
GAAGGGGGATGCTTAGATCT+TGG + chr4.1:27109568-27109587 MS.gene065904:CDS 50.0%
GATGGAAACAAGTGCGGCAT+TGG - chr4.1:27109695-27109714 None:intergenic 50.0%
GCAAGGATCGCTCCATTGTT+AGG - chr4.1:27108937-27108956 None:intergenic 50.0%
GCACATCAAAGAAGTGGAGG+TGG + chr4.1:27109543-27109562 MS.gene065904:intron 50.0%
TTGAAGGAGATGGAGGGGTT+GGG + chr4.1:27108629-27108648 MS.gene065904:intron 50.0%
ACGTGCCAGCAAGTGAAGCA+TGG + chr4.1:27108530-27108549 MS.gene065904:CDS 55.0%
CCACTAGGTCAACGCCTCAA+CGG - chr4.1:27108814-27108833 None:intergenic 55.0%
CGTGCCAGCAAGTGAAGCAT+GGG + chr4.1:27108531-27108550 MS.gene065904:CDS 55.0%
GAAGTGGAGGTGGTTGAAGG+GGG + chr4.1:27109553-27109572 MS.gene065904:CDS 55.0%
TCAGGTTTACCTGGCCCAAC+TGG + chr4.1:27109484-27109503 MS.gene065904:intron 55.0%
! CGCCGCTAGACCTTTTTGGA+TGG - chr4.1:27109104-27109123 None:intergenic 55.0%
CTAGGTCAACGCCTCAACGG+AGG - chr4.1:27108811-27108830 None:intergenic 60.0%
! GCTGCGCCGCTAGACCTTTT+TGG - chr4.1:27109108-27109127 None:intergenic 60.0%
!! CCGTTGAGGCGTTGACCTAG+TGG + chr4.1:27108811-27108830 MS.gene065904:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 27108496 27109783 27108496 ID=MS.gene065904
chr4.1 mRNA 27108496 27109783 27108496 ID=MS.gene065904.t1;Parent=MS.gene065904
chr4.1 exon 27108496 27108645 27108496 ID=MS.gene065904.t1.exon1;Parent=MS.gene065904.t1
chr4.1 CDS 27108496 27108645 27108496 ID=cds.MS.gene065904.t1;Parent=MS.gene065904.t1
chr4.1 exon 27109544 27109783 27109544 ID=MS.gene065904.t1.exon2;Parent=MS.gene065904.t1
chr4.1 CDS 27109544 27109783 27109544 ID=cds.MS.gene065904.t1;Parent=MS.gene065904.t1
Gene Sequence

>MS.gene065904

ATGTTTGGTCAACTTGAGCATGAGTTAGAGCTGCACGTGCCAGCAAGTGAAGCATGGGATCTCTTTGGCACTCTTGCTATTGGAAAGGTCGTTGAGGAAGCGATGCCTGAAAGATTTCAGAAGGTGGAGCTAATTGAAGGAGATGGAGGGGTTGGGACTATTCTCAAACTCACATTCACTCCAGGTTATTTTCATTATTATTTATGTATTCTTTATATTAACTTGCCACAATTATACTCAAAAATATAATTATCGTTACATAATTCATATGGAAAATTTAATGTAAATTTATTCCATCAATTGTAAACACATCCTCCGTTGAGGCGTTGACCTAGTGGAGAATAAGGTGGTTAGAAATTCATATTAGATATTCATCTTAAGCTGAATAAGTGGAGAATCTAGATTTGAACCTGATCCTTACATATCAAATGTGATGTCCCTAACAATGGAGCGATCCTTGCCGAGACTACTTTCTATTTAATTTTATTTTGATCATATGACTATTGTATCATGAACATTTTGGATTAGTTTTGAACATCTTTTCATGCATAGCAATGTCATGTCTCCTAGGTCCATGAATGTTGTGTTCACGATGATTGATTTTGCCATCCAAAAAGGTCTAGCGGCGCAGCTTGTTGCCCGCTTGCCTTTTATTCAAGTGTAAATAATGGTTTATATATATATATATATATATATATATATATATATATATATATATATATAATAAAATAAAAAATAAAACTGCAAGTGACTTTTAATAGATTGATCCACCATTATGTCTATGTACAGGTGGGGGATTCGGATGTATTTCAATCTATAGATTCATGAGTTTGGAAAGAATTTAAATTAAAGATATGATGAATTAACATACTTACGGGACAATACTATATGTATACAAGTCTATAATATTTTGTGTTAACATTCTTTCAAAAAAAAAAAAAAAGTATTTTGTGTTAACATAACATTAATTGTTTTGGTCGGAGTTTGATCAGGTTTACCTGGCCCAACTGGTTTAAAAGAAAAGTTTACCAAGATTGATAATGAAAAGCACATCAAAGAAGTGGAGGTGGTTGAAGGGGGATGCTTAGATCTTGGATTCACACTATTTAGAGTTCGTATGGAAGTGATAGAGAAAGGTAAAGATTCAAGCATAATCAAAACTACATTAGAGTATGAAGTCAAGGAAGAGGAAGCGGCCAATGCCGCACTTGTTTCCATCCAGCAATTGGCAGACGTTGTAGAAGTTGGCAAGAATTACCTCAACAGAAACAAGGATGCAAAAGAATAA

Protein sequence

>MS.gene065904.t1

MFGQLEHELELHVPASEAWDLFGTLAIGKVVEEAMPERFQKVELIEGDGGHIKEVEVVEGGCLDLGFTLFRVRMEVIEKGKDSSIIKTTLEYEVKEEEAANAALVSIQQLADVVEVGKNYLNRNKDAKE