AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74364


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74364.t1 MTR_8g045400 78.981 157 15 2 1 140 1 156 2.06e-82 239
MS.gene74364.t1 MTR_8g045520 68.790 157 32 1 1 140 1 157 1.14e-71 212
MS.gene74364.t1 MTR_8g045490 67.974 153 32 1 1 136 1 153 3.96e-71 210
MS.gene74364.t1 MTR_8g045695 65.605 157 36 2 1 140 1 156 1.50e-68 204
MS.gene74364.t1 MTR_8g045570 63.816 152 38 1 1 135 1 152 4.07e-66 197
MS.gene74364.t1 MTR_8g045665 64.331 157 38 2 1 140 1 156 3.92e-65 195
MS.gene74364.t1 MTR_8g045640 66.242 157 35 2 1 140 1 156 4.37e-65 195
MS.gene74364.t1 MTR_8g045735 63.871 155 38 2 1 138 1 154 8.81e-65 194
MS.gene74364.t1 MTR_8g045560 64.968 157 37 2 1 140 1 156 2.12e-64 193
MS.gene74364.t1 MTR_8g045300 62.179 156 42 1 1 139 1 156 5.08e-64 192
MS.gene74364.t1 MTR_8g045555 61.184 152 42 1 1 135 1 152 4.58e-61 185
MS.gene74364.t1 MTR_1g030820 34.868 152 81 2 1 134 1 152 3.81e-27 99.0
MS.gene74364.t1 MTR_1g030810 34.211 152 82 2 1 134 1 152 7.99e-26 95.5
MS.gene74364.t1 MTR_1g030840 32.903 155 81 4 1 132 1 155 2.49e-20 82.0
MS.gene74364.t1 MTR_8g012550 31.250 144 81 3 6 132 8 150 5.08e-20 80.9
MS.gene74364.t1 MTR_1g031640 30.612 147 78 4 7 135 7 147 1.25e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74364.t1 AT5G28010 30.921 152 87 3 3 136 15 166 5.78e-19 78.6
MS.gene74364.t1 AT5G28010 30.921 152 87 3 3 136 15 166 5.78e-19 78.6
MS.gene74364.t1 AT1G70890 32.895 152 84 3 3 136 7 158 2.00e-17 74.3
MS.gene74364.t1 AT1G70830 32.237 152 85 3 3 136 22 173 3.88e-17 73.9
MS.gene74364.t1 AT1G70840 30.921 152 87 3 3 136 20 171 5.12e-17 73.6
MS.gene74364.t1 AT1G70870 35.252 139 84 3 1 134 1 138 5.28e-17 72.8
MS.gene74364.t1 AT1G70830 32.237 152 85 3 3 136 22 173 7.11e-17 73.9
MS.gene74364.t1 AT1G70830 32.237 152 85 3 3 136 22 173 3.19e-16 72.8
MS.gene74364.t1 AT1G70830 32.237 152 85 3 3 136 22 173 3.79e-16 73.6
MS.gene74364.t1 AT1G70830 30.921 152 87 3 3 136 184 335 1.63e-14 69.3
MS.gene74364.t1 AT1G23120 37.500 88 54 1 46 133 59 145 3.07e-15 68.2
MS.gene74364.t1 AT1G23120 37.500 88 54 1 46 133 109 195 4.09e-15 68.9
MS.gene74364.t1 AT1G70830 30.921 152 87 3 3 136 137 288 6.49e-15 69.7
MS.gene74364.t1 AT5G28000 29.605 152 89 3 3 136 13 164 7.52e-15 67.8
MS.gene74364.t1 AT1G70850 30.000 150 87 3 3 134 9 158 1.10e-14 68.6
MS.gene74364.t1 AT1G70850 30.201 149 86 3 3 133 9 157 1.64e-14 68.9
MS.gene74364.t1 AT1G70850 30.986 142 80 3 13 136 175 316 1.34e-13 66.6
MS.gene74364.t1 AT1G70850 30.201 149 86 3 3 133 9 157 1.64e-14 68.9
MS.gene74364.t1 AT1G70850 30.986 142 80 3 13 136 175 316 1.34e-13 66.6
MS.gene74364.t1 AT1G24020 30.921 152 86 4 1 134 1 151 8.62e-14 64.7
MS.gene74364.t1 AT1G24020 30.921 152 86 4 1 134 1 151 8.62e-14 64.7
MS.gene74364.t1 AT1G70880 27.632 152 92 3 3 136 8 159 1.02e-13 64.7
MS.gene74364.t1 AT4G23670 29.032 155 86 4 1 135 1 151 2.91e-12 60.5
MS.gene74364.t1 AT1G14940 27.857 140 99 2 1 139 1 139 6.54e-12 59.3

Find 26 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTCACCTTCATGAAGTT+TGG 0.292428 8.4:-55913191 None:intergenic
AATGGCTGGATGGACTTATT+AGG 0.345844 8.4:+55914173 MS.gene74364:CDS
CATTCAGAAATGGTGCTTGC+TGG 0.350620 8.4:+55913061 None:intergenic
CACCCTTATCAATTACTTCA+AGG 0.382574 8.4:-55914085 None:intergenic
AATAAGTCCATCCAGCCATT+TGG 0.421732 8.4:-55914170 None:intergenic
AAAATAACTTTCAAGCTCTT+CGG 0.428417 8.4:+55914023 MS.gene74364:CDS
CATCCTTGAAGTAATTGATA+AGG 0.438738 8.4:+55914082 MS.gene74364:CDS
ATGGCAGTGGAGCTGCAAAA+TGG 0.452179 8.4:+55914108 MS.gene74364:CDS
ATCCTTGAAGTAATTGATAA+GGG 0.486541 8.4:+55914083 MS.gene74364:CDS
AGACATTGATGAGCACTATA+AGG 0.495099 8.4:+55914049 MS.gene74364:CDS
AGTTTGAAGAACTTGTCAAC+GGG 0.511180 8.4:-55913115 None:intergenic
ATTGATAAGGGTGATGGCAG+TGG 0.521797 8.4:+55914095 MS.gene74364:CDS
ATATTGACCCTCCAAATGGC+TGG 0.527845 8.4:+55914159 MS.gene74364:CDS
TGACATCACAATAGATGGTA+AGG 0.533449 8.4:+55913953 MS.gene74364:intron
GGTAAACTTAGTACTGAACT+TGG 0.538495 8.4:+55913082 MS.gene74364:CDS
GAGGATATTGACCCTCCAAA+TGG 0.539495 8.4:+55914155 MS.gene74364:CDS
CTTCATGCCAAACTTCATGA+AGG 0.543027 8.4:+55913184 MS.gene74364:CDS
GAAGTAATTGATAAGGGTGA+TGG 0.549119 8.4:+55914089 MS.gene74364:CDS
AAGTCCATCCAGCCATTTGG+AGG 0.551025 8.4:-55914167 None:intergenic
ATAACTTTCAAGCTCTTCGG+TGG 0.552870 8.4:+55914026 MS.gene74364:CDS
TGACCCTCCAAATGGCTGGA+TGG 0.557517 8.4:+55914163 MS.gene74364:CDS
GTTGATGCTCATCTTGTCAA+AGG 0.600604 8.4:+55914212 MS.gene74364:CDS
TAGTTTGAAGAACTTGTCAA+CGG 0.601012 8.4:-55913116 None:intergenic
GTAAACTTAGTACTGAACTT+GGG 0.614711 8.4:+55913083 MS.gene74364:CDS
AGTCCATCCAGCCATTTGGA+GGG 0.649199 8.4:-55914166 None:intergenic
TGAATATGAAAAGATCAATG+AGG 0.667026 8.4:+55914136 MS.gene74364:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATACATTGTTTTAATTTAAT+TGG + chr8.4:55913917-55913936 MS.gene74364:intron 10.0%
!! ACAATATATAGAATAAGTGA+TGG + chr8.4:55913569-55913588 MS.gene74364:intron 20.0%
!! CATAATCTTCGTATAAAATA+TGG - chr8.4:55913355-55913374 None:intergenic 20.0%
!! GATTTACATGTCTAAATAAA+TGG + chr8.4:55913622-55913641 MS.gene74364:intron 20.0%
!! GTAGAGTTATGAAAAATATA+TGG - chr8.4:55913897-55913916 None:intergenic 20.0%
!! TACATACTTGGTATAAAATT+AGG + chr8.4:55913665-55913684 MS.gene74364:intron 20.0%
!! TGCTCTTATTAATTGAATTA+AGG - chr8.4:55913326-55913345 None:intergenic 20.0%
!!! AATTCAATTAATAAGAGCAT+AGG + chr8.4:55913327-55913346 MS.gene74364:intron 20.0%
!!! ATTCAATTAATAAGAGCATA+GGG + chr8.4:55913328-55913347 MS.gene74364:intron 20.0%
! AAAATAACTTTCAAGCTCTT+CGG + chr8.4:55914023-55914042 MS.gene74364:CDS 25.0%
! AAATAAACAAGATGATGACT+TGG - chr8.4:55913293-55913312 None:intergenic 25.0%
! ACTTTATCTTTGTCATCTTT+AGG - chr8.4:55913379-55913398 None:intergenic 25.0%
! ATCCTTGAAGTAATTGATAA+GGG + chr8.4:55914083-55914102 MS.gene74364:CDS 25.0%
! TGAATATGAAAAGATCAATG+AGG + chr8.4:55914136-55914155 MS.gene74364:CDS 25.0%
!! TATTGTCTCATTGATTGATT+TGG - chr8.4:55913555-55913574 None:intergenic 25.0%
AAAAATAAGACACTGTTTCC+TGG + chr8.4:55913425-55913444 MS.gene74364:intron 30.0%
ACAAATTCATATATGATGCC+AGG - chr8.4:55913446-55913465 None:intergenic 30.0%
ATAGATACGTAGTACATACT+TGG + chr8.4:55913653-55913672 MS.gene74364:intron 30.0%
CATATATGAATTTGTGCAGA+TGG + chr8.4:55913451-55913470 MS.gene74364:intron 30.0%
CATCCTTGAAGTAATTGATA+AGG + chr8.4:55914082-55914101 MS.gene74364:CDS 30.0%
TAGTTTGAAGAACTTGTCAA+CGG - chr8.4:55913119-55913138 None:intergenic 30.0%
! GTAAACTTAGTACTGAACTT+GGG + chr8.4:55913083-55913102 MS.gene74364:CDS 30.0%
AACTTCATGAAGGTGAAGAT+TGG + chr8.4:55913194-55913213 MS.gene74364:intron 35.0%
ACCATCTATTGTGATGTCAT+GGG - chr8.4:55913952-55913971 None:intergenic 35.0%
AGACATTGATGAGCACTATA+AGG + chr8.4:55914049-55914068 MS.gene74364:CDS 35.0%
AGTTTGAAGAACTTGTCAAC+GGG - chr8.4:55913118-55913137 None:intergenic 35.0%
ATATGAATTTGTGCAGATGG+AGG + chr8.4:55913454-55913473 MS.gene74364:intron 35.0%
ATCTTCACCTTCATGAAGTT+TGG - chr8.4:55913194-55913213 None:intergenic 35.0%
CACCCTTATCAATTACTTCA+AGG - chr8.4:55914088-55914107 None:intergenic 35.0%
GAAGTAATTGATAAGGGTGA+TGG + chr8.4:55914089-55914108 MS.gene74364:CDS 35.0%
TACCATCTATTGTGATGTCA+TGG - chr8.4:55913953-55913972 None:intergenic 35.0%
TGACATCACAATAGATGGTA+AGG + chr8.4:55913953-55913972 MS.gene74364:intron 35.0%
! GGTAAACTTAGTACTGAACT+TGG + chr8.4:55913082-55913101 MS.gene74364:CDS 35.0%
!! AAGATGATGACTTGGTTATG+AGG - chr8.4:55913285-55913304 None:intergenic 35.0%
!! ACACTGATTCAGTTAAACAC+TGG + chr8.4:55913221-55913240 MS.gene74364:intron 35.0%
AATAAGTCCATCCAGCCATT+TGG - chr8.4:55914173-55914192 None:intergenic 40.0%
ATAACTTTCAAGCTCTTCGG+TGG + chr8.4:55914026-55914045 MS.gene74364:CDS 40.0%
CTTCATGCCAAACTTCATGA+AGG + chr8.4:55913184-55913203 MS.gene74364:CDS 40.0%
GTCCTAAACGATATGTGAGA+AGG + chr8.4:55913765-55913784 MS.gene74364:intron 40.0%
GTTGATGCTCATCTTGTCAA+AGG + chr8.4:55914212-55914231 MS.gene74364:CDS 40.0%
TCCCATGACATCACAATAGA+TGG + chr8.4:55913948-55913967 MS.gene74364:intron 40.0%
TGCCTTCTCACATATCGTTT+AGG - chr8.4:55913770-55913789 None:intergenic 40.0%
! AATGGCTGGATGGACTTATT+AGG + chr8.4:55914173-55914192 MS.gene74364:CDS 40.0%
ATATTGACCCTCCAAATGGC+TGG + chr8.4:55914159-55914178 MS.gene74364:CDS 45.0%
GAGGATATTGACCCTCCAAA+TGG + chr8.4:55914155-55914174 MS.gene74364:CDS 45.0%
TTGTGCAGATGGAGGATCTA+TGG + chr8.4:55913462-55913481 MS.gene74364:intron 45.0%
! ATTGATAAGGGTGATGGCAG+TGG + chr8.4:55914095-55914114 MS.gene74364:CDS 45.0%
AAGTCCATCCAGCCATTTGG+AGG - chr8.4:55914170-55914189 None:intergenic 50.0%
AGTCCATCCAGCCATTTGGA+GGG - chr8.4:55914169-55914188 None:intergenic 50.0%
ATGGCAGTGGAGCTGCAAAA+TGG + chr8.4:55914108-55914127 MS.gene74364:CDS 50.0%
CAGATGGAGGATCTATGGAG+AGG + chr8.4:55913467-55913486 MS.gene74364:intron 50.0%
TGACCCTCCAAATGGCTGGA+TGG + chr8.4:55914163-55914182 MS.gene74364:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 55913070 55914253 55913070 ID=MS.gene74364
chr8.4 mRNA 55913070 55914253 55913070 ID=MS.gene74364.t1;Parent=MS.gene74364
chr8.4 exon 55913070 55913205 55913070 ID=MS.gene74364.t1.exon1;Parent=MS.gene74364.t1
chr8.4 CDS 55913070 55913205 55913070 ID=cds.MS.gene74364.t1;Parent=MS.gene74364.t1
chr8.4 exon 55913967 55914253 55913967 ID=MS.gene74364.t1.exon2;Parent=MS.gene74364.t1
chr8.4 CDS 55913967 55914253 55913967 ID=cds.MS.gene74364.t1;Parent=MS.gene74364.t1
Gene Sequence

>MS.gene74364

ATGGTGCTTGCTGGTAAACTTAGTACTGAACTTGGGATCAAAACACCCGTTGACAAGTTCTTCAAACTATATGCATCAGAACTTCACGAAGTGCAAAACATTAGTGAAAGAATTCTTCATGCCAAACTTCATGAAGGTGAAGATTGGCATCACACTGATTCAGTTAAACACTGGACTTATGTCATAGTTAATTAAGTTTCTAATTCAATTGACCTCATAACCAAGTCATCATCTTGTTTATTTATATATTGATCCTTAATTCAATTAATAAGAGCATAGGGACCATATTTTATACGAAGATTATGTCCTAAAGATGACAAAGATAAAGTTTTTCAGCTGTGAAATTTAGATACAAAAAAATAAGACACTGTTTCCTGGCATCATATATGAATTTGTGCAGATGGAGGATCTATGGAGAGGAAAAGATAAAAAAAAAATAAAACAGTCAGAAAAAAAAAACAATTCGATCAAAGTTTAATTAACCAAATCAATCAATGAGACAATATATAGAATAAGTGATGGATTTATAGATACATCATAGTACATATACTTGATTTACATGTCTAAATAAATGGTAGTTACTATAGATACGTAGTACATACTTGGTATAAAATTAGGAGATACATGATTGACTTTATAGTTGTAATGAGTGCATATAATTCAGACTTCTTAAAGATATTCAAATATTGCATAGAGTCCTAAACGATATGTGAGAAGGCAACTAAACATTATATAGATTATTAATATTCATTTTTTTGTTTAAAAACTATTTTATTTGAAAAATGAAAACAATTTAATTGATTAGTTATTTTAAAAAAATGTGTCCATATATTTTTCATAACTCTACATACATTGTTTTAATTTAATTGGTATTAATTTCCCATGACATCACAATAGATGGTAAGGTAAACACATGTCATGAGAGTGTTGAAGAAGTTGATGAACACAACAAAAAAATAACTTTCAAGCTCTTCGGTGGAGACATTGATGAGCACTATAAGGTCTTTAAGCTCATCCTTGAAGTAATTGATAAGGGTGATGGCAGTGGAGCTGCAAAATGGACAGTTGAATATGAAAAGATCAATGAGGATATTGACCCTCCAAATGGCTGGATGGACTTATTAGGCAAAGAAACTCGAGAAGTTGATGCTCATCTTGTCAAAGGCGAAAACATAGCTCTATAA

Protein sequence

>MS.gene74364.t1

MVLAGKLSTELGIKTPVDKFFKLYASELHEVQNISERILHAKLHEDGKVNTCHESVEEVDEHNKKITFKLFGGDIDEHYKVFKLILEVIDKGDGSGAAKWTVEYEKINEDIDPPNGWMDLLGKETREVDAHLVKGENIAL