AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23190.t1 MTR_7g451810 97.015 134 4 0 1 134 152 285 1.06e-92 270
MS.gene23190.t1 MTR_7g047630 97.015 134 4 0 1 134 152 285 1.06e-92 270
MS.gene23190.t1 MTR_7g047660 59.701 67 20 2 66 132 76 135 4.22e-18 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23190.t1 AT4G05210 80.469 128 25 0 1 128 158 285 3.34e-73 220
MS.gene23190.t1 AT4G05210 80.469 128 25 0 1 128 176 303 8.98e-73 220
MS.gene23190.t1 AT4G21220 79.200 125 26 0 1 125 169 293 1.71e-70 214
MS.gene23190.t1 AT4G05210 67.320 153 25 1 1 128 170 322 4.21e-67 206

Find 35 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGGAGAGATACATTTAT+TGG 0.239574 7.2:-51816546 MS.gene23190:CDS
ATTGGCCACAATGTAGTTAT+TGG 0.252137 7.2:-51816414 MS.gene23190:CDS
ATCACAGAACCTGGAGATTA+TGG 0.315331 7.2:-51815618 MS.gene23190:CDS
TGTGGACAAGTTGGGATTGC+AGG 0.325045 7.2:-51816375 MS.gene23190:CDS
CAGAAGCTCAATGTAATAGT+AGG 0.355997 7.2:-51816965 MS.gene23190:CDS
GTAGGGAACAATGTGGAAAT+TGG 0.367733 7.2:-51816947 MS.gene23190:CDS
GAAGATAGATAATTTAGTTC+AGG 0.370626 7.2:-51816514 MS.gene23190:intron
GCTGGAGAGATACATTTATT+GGG 0.371063 7.2:-51816545 MS.gene23190:CDS
TTGGCCACAATGTAGTTATT+GGG 0.373724 7.2:-51816413 MS.gene23190:CDS
TCCAGGTTCTGTGATGTCCT+TGG 0.461823 7.2:+51815626 None:intergenic
GTGCAAATACATGCATTGAC+AGG 0.462960 7.2:-51816925 MS.gene23190:CDS
ATTATGTGACGATGGGAGGA+AGG 0.470760 7.2:-51816214 MS.gene23190:CDS
TGTAGGTTTCTATTCACAAA+TGG 0.471353 7.2:-51815456 MS.gene23190:intron
ACCAAGGACATCACAGAACC+TGG 0.502358 7.2:-51815627 MS.gene23190:CDS
GCATGCTATGTGGACAAGTT+GGG 0.509568 7.2:-51816383 MS.gene23190:CDS
TGCATGCTATGTGGACAAGT+TGG 0.510465 7.2:-51816384 MS.gene23190:CDS
TCCTCTAACTGCAAAACTGC+TGG 0.516609 7.2:+51815420 None:intergenic
TCATGTATCGATTGCATCAA+AGG 0.517806 7.2:-51816177 MS.gene23190:intron
ATACATGCATTGACAGGGGC+AGG 0.537225 7.2:-51816919 MS.gene23190:intron
AGAAGCTCAATGTAATAGTA+GGG 0.553263 7.2:-51816964 MS.gene23190:CDS
GGAAAGCCACCATAATCTCC+AGG 0.557434 7.2:+51815609 None:intergenic
TGCAAATACATGCATTGACA+GGG 0.560693 7.2:-51816924 MS.gene23190:CDS
GGAGATTATGTGACGATGGG+AGG 0.576832 7.2:-51816218 MS.gene23190:CDS
CATAGGAGATTATGTGACGA+TGG 0.588173 7.2:-51816222 MS.gene23190:CDS
TTATGTGACGATGGGAGGAA+GGG 0.590326 7.2:-51816213 MS.gene23190:CDS
AGGTTTCTATTCACAAATGG+CGG 0.594531 7.2:-51815453 MS.gene23190:CDS
ATAGGAGATTATGTGACGAT+GGG 0.605328 7.2:-51816221 MS.gene23190:CDS
CTGGAGAGATACATTTATTG+GGG 0.609991 7.2:-51816544 MS.gene23190:CDS
TGCGTTAAGTTGTGTAACCA+AGG 0.629797 7.2:-51815643 MS.gene23190:CDS
TAACATGATAAAGAAGCCAC+AGG 0.643538 7.2:-51817109 None:intergenic
TCTTCCCAATAACTACATTG+TGG 0.648401 7.2:+51816409 None:intergenic
GGGAAGAATTGCATGCTATG+TGG 0.655876 7.2:-51816393 MS.gene23190:CDS
ACAGAACCTGGAGATTATGG+TGG 0.680720 7.2:-51815615 MS.gene23190:CDS
TGTAATAGTAGGGAACAATG+TGG 0.718516 7.2:-51816954 MS.gene23190:CDS
GCAAATACATGCATTGACAG+GGG 0.723551 7.2:-51816923 MS.gene23190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTACTGTTTGTAAATT+GGG - chr7.2:51816797-51816816 MS.gene23190:intron 15.0%
!!! TATTTTTACTGTTTGTAAAT+TGG - chr7.2:51816796-51816815 MS.gene23190:intron 15.0%
!! AACTAATTATATTCACTCAT+TGG - chr7.2:51815944-51815963 MS.gene23190:intron 20.0%
!! AAGAAACTTTCAACATATAT+AGG + chr7.2:51815466-51815485 None:intergenic 20.0%
!! ATATCAAACTATAATGATCT+TGG - chr7.2:51816463-51816482 MS.gene23190:intron 20.0%
!! ATTAGTTAGAAAATTACAAG+AGG - chr7.2:51815759-51815778 MS.gene23190:intron 20.0%
!! TTAAAGATTTGAAAGACATT+GGG - chr7.2:51816500-51816519 MS.gene23190:intron 20.0%
!!! ACATGTTTTTTTTTTACAAG+AGG - chr7.2:51816530-51816549 MS.gene23190:CDS 20.0%
!!! GTTGTTATCTAGTAAATTTT+GGG - chr7.2:51816198-51816217 MS.gene23190:CDS 20.0%
!!! TGTTGTTATCTAGTAAATTT+TGG - chr7.2:51816197-51816216 MS.gene23190:CDS 20.0%
! ATCATGAAGCTAAATAGAAA+AGG + chr7.2:51815648-51815667 None:intergenic 25.0%
! GTTAAAGATTTGAAAGACAT+TGG - chr7.2:51816499-51816518 MS.gene23190:intron 25.0%
! TATGAAAAGAGCTAAATTTC+AGG + chr7.2:51816576-51816595 None:intergenic 25.0%
! TCAATTGATCATTTGAATAG+CGG + chr7.2:51816747-51816766 None:intergenic 25.0%
!! GAAGATAGATAATTTAGTTC+AGG - chr7.2:51816005-51816024 MS.gene23190:intron 25.0%
AGAAGCTCAATGTAATAGTA+GGG - chr7.2:51815555-51815574 MS.gene23190:intron 30.0%
ATCATGAAGATATGAGTGAA+AGG - chr7.2:51815713-51815732 MS.gene23190:intron 30.0%
TGACATGCATACATGTTATT+GGG - chr7.2:51817005-51817024 MS.gene23190:intron 30.0%
TGTAGGTTTCTATTCACAAA+TGG - chr7.2:51817063-51817082 MS.gene23190:intron 30.0%
TTGACATGCATACATGTTAT+TGG - chr7.2:51817004-51817023 MS.gene23190:intron 30.0%
!!! AAATTTTGGGATGATACATC+TGG - chr7.2:51816211-51816230 MS.gene23190:CDS 30.0%
!!! AGGTATGCTTTTGTTTTACT+TGG - chr7.2:51816362-51816381 MS.gene23190:intron 30.0%
ACAGAGAAAAATGTAAGCCT+CGG + chr7.2:51816266-51816285 None:intergenic 35.0%
AGCTGGAGAGATACATTTAT+TGG - chr7.2:51815973-51815992 MS.gene23190:intron 35.0%
AGGTTTCTATTCACAAATGG+CGG - chr7.2:51817066-51817085 MS.gene23190:intron 35.0%
ATAGGAGATTATGTGACGAT+GGG - chr7.2:51816298-51816317 MS.gene23190:intron 35.0%
ATGCATACATGTTATTGGGT+TGG - chr7.2:51817009-51817028 MS.gene23190:intron 35.0%
ATTGGCCACAATGTAGTTAT+TGG - chr7.2:51816105-51816124 MS.gene23190:intron 35.0%
CAGAAGCTCAATGTAATAGT+AGG - chr7.2:51815554-51815573 MS.gene23190:intron 35.0%
CTGGAGAGATACATTTATTG+GGG - chr7.2:51815975-51815994 MS.gene23190:intron 35.0%
GCTGGAGAGATACATTTATT+GGG - chr7.2:51815974-51815993 MS.gene23190:intron 35.0%
TACTAAGTGCATGTTTGCTT+TGG - chr7.2:51816672-51816691 MS.gene23190:intron 35.0%
TAGAAACCTACAAAGCAACA+AGG + chr7.2:51817055-51817074 None:intergenic 35.0%
TAGAACTAAAGTTATTGCCG+AGG - chr7.2:51816246-51816265 MS.gene23190:intron 35.0%
TCTTCCCAATAACTACATTG+TGG + chr7.2:51816113-51816132 None:intergenic 35.0%
TGCATACATGTTATTGGGTT+GGG - chr7.2:51817010-51817029 MS.gene23190:intron 35.0%
TGTAATAGTAGGGAACAATG+TGG - chr7.2:51815565-51815584 MS.gene23190:intron 35.0%
TGTTTGTAAATTGGGTGCTT+GGG - chr7.2:51816805-51816824 MS.gene23190:intron 35.0%
TTGGCCACAATGTAGTTATT+GGG - chr7.2:51816106-51816125 MS.gene23190:intron 35.0%
! AGTTTTCATCTTCACACTCA+CGG - chr7.2:51816617-51816636 MS.gene23190:intron 35.0%
! TGCAAATACATGCATTGACA+GGG - chr7.2:51815595-51815614 MS.gene23190:intron 35.0%
!! TCATGTATCGATTGCATCAA+AGG - chr7.2:51816342-51816361 MS.gene23190:intron 35.0%
!! TTCTGATTAGTTCTGCAGAT+TGG - chr7.2:51816087-51816106 MS.gene23190:intron 35.0%
!!! TTGTTTTACTTGGTGCATTG+AGG - chr7.2:51816372-51816391 MS.gene23190:CDS 35.0%
AGTTAGAAACTCTGATGAGC+TGG + chr7.2:51815811-51815830 None:intergenic 40.0%
ATCACAGAACCTGGAGATTA+TGG - chr7.2:51816901-51816920 MS.gene23190:intron 40.0%
ATGATAAAGAAGCCACAGGT+TGG - chr7.2:51815414-51815433 MS.gene23190:CDS 40.0%
CAAGACAAATTACCAACCTG+TGG + chr7.2:51815429-51815448 None:intergenic 40.0%
CATAGGAGATTATGTGACGA+TGG - chr7.2:51816297-51816316 MS.gene23190:intron 40.0%
CGGTGAGTATGACAAAATCA+CGG + chr7.2:51816727-51816746 None:intergenic 40.0%
CTCTGTTATGTGTTCAGCAT+AGG - chr7.2:51816280-51816299 MS.gene23190:intron 40.0%
CTGTTTGTAAATTGGGTGCT+TGG - chr7.2:51816804-51816823 MS.gene23190:intron 40.0%
GACTAGTAGATCATTACAGC+AGG + chr7.2:51816934-51816953 None:intergenic 40.0%
GTAGGGAACAATGTGGAAAT+TGG - chr7.2:51815572-51815591 MS.gene23190:intron 40.0%
GTTTGTAAATTGGGTGCTTG+GGG - chr7.2:51816806-51816825 MS.gene23190:intron 40.0%
TAAGTGCATGTTTGCTTTGG+TGG - chr7.2:51816675-51816694 MS.gene23190:intron 40.0%
TGAGTATGACAAAATCACGG+TGG + chr7.2:51816724-51816743 None:intergenic 40.0%
! CAAGGAAGCTGGTTTATGAA+TGG + chr7.2:51817037-51817056 None:intergenic 40.0%
! GCAAATACATGCATTGACAG+GGG - chr7.2:51815596-51815615 MS.gene23190:intron 40.0%
! GTGCAAATACATGCATTGAC+AGG - chr7.2:51815594-51815613 MS.gene23190:intron 40.0%
! TGCGTTAAGTTGTGTAACCA+AGG - chr7.2:51816876-51816895 MS.gene23190:intron 40.0%
!! TTTACTTGGTGCATTGAGGA+TGG - chr7.2:51816376-51816395 MS.gene23190:CDS 40.0%
AAACTCTGATGAGCTGGCAT+AGG + chr7.2:51815805-51815824 None:intergenic 45.0%
AATGTATCTCTCCAGCTGCA+TGG + chr7.2:51815970-51815989 None:intergenic 45.0%
ACAGAACCTGGAGATTATGG+TGG - chr7.2:51816904-51816923 MS.gene23190:intron 45.0%
ATTATGTGACGATGGGAGGA+AGG - chr7.2:51816305-51816324 MS.gene23190:intron 45.0%
CACAACCTGTAATTCCTTGC+TGG - chr7.2:51816646-51816665 MS.gene23190:intron 45.0%
CTACAAAGCAACAAGGAAGC+TGG + chr7.2:51817048-51817067 None:intergenic 45.0%
GCATGCTATGTGGACAAGTT+GGG - chr7.2:51816136-51816155 MS.gene23190:intron 45.0%
GGGAAGAATTGCATGCTATG+TGG - chr7.2:51816126-51816145 MS.gene23190:intron 45.0%
TACAGCCAGCAAGGAATTAC+AGG + chr7.2:51816654-51816673 None:intergenic 45.0%
TCCTCTAACTGCAAAACTGC+TGG + chr7.2:51817102-51817121 None:intergenic 45.0%
TGCATGCTATGTGGACAAGT+TGG - chr7.2:51816135-51816154 MS.gene23190:intron 45.0%
TTATGTGACGATGGGAGGAA+GGG - chr7.2:51816306-51816325 MS.gene23190:intron 45.0%
!! CAGCTTCCTTGTTGCTTTGT+AGG - chr7.2:51817046-51817065 MS.gene23190:intron 45.0%
ACCAAGGACATCACAGAACC+TGG - chr7.2:51816892-51816911 MS.gene23190:intron 50.0%
GGAAAGCCACCATAATCTCC+AGG + chr7.2:51816913-51816932 None:intergenic 50.0%
GGAGATTATGTGACGATGGG+AGG - chr7.2:51816301-51816320 MS.gene23190:intron 50.0%
GGTGAGTTCAGCAGAATCAC+TGG - chr7.2:51816696-51816715 MS.gene23190:intron 50.0%
TCCAGGTTCTGTGATGTCCT+TGG + chr7.2:51816896-51816915 None:intergenic 50.0%
TCTGATTGCAACGTGCTTGC+AGG - chr7.2:51816843-51816862 MS.gene23190:intron 50.0%
TGCACTTAGTACAGCCAGCA+AGG + chr7.2:51816663-51816682 None:intergenic 50.0%
! ATACATGCATTGACAGGGGC+AGG - chr7.2:51815600-51815619 MS.gene23190:intron 50.0%
! CTTGGTGCATTGAGGATGGT+TGG - chr7.2:51816380-51816399 MS.gene23190:CDS 50.0%
! GCCAGCAGTTTTGCAGTTAG+AGG - chr7.2:51817098-51817117 MS.gene23190:intron 50.0%
! TGTGGACAAGTTGGGATTGC+AGG - chr7.2:51816144-51816163 MS.gene23190:intron 50.0%
TCACTCATTGGCCATGCAGC+TGG - chr7.2:51815956-51815975 MS.gene23190:intron 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 51815414 51817127 51815414 ID=MS.gene23190
chr7.2 mRNA 51815414 51817127 51815414 ID=MS.gene23190.t1;Parent=MS.gene23190
chr7.2 exon 51817110 51817127 51817110 ID=MS.gene23190.t1.exon1;Parent=MS.gene23190.t1
chr7.2 CDS 51817110 51817127 51817110 ID=cds.MS.gene23190.t1;Parent=MS.gene23190.t1
chr7.2 exon 51816920 51816984 51816920 ID=MS.gene23190.t1.exon2;Parent=MS.gene23190.t1
chr7.2 CDS 51816920 51816984 51816920 ID=cds.MS.gene23190.t1;Parent=MS.gene23190.t1
chr7.2 exon 51816515 51816566 51816515 ID=MS.gene23190.t1.exon3;Parent=MS.gene23190.t1
chr7.2 CDS 51816515 51816566 51816515 ID=cds.MS.gene23190.t1;Parent=MS.gene23190.t1
chr7.2 exon 51816366 51816436 51816366 ID=MS.gene23190.t1.exon4;Parent=MS.gene23190.t1
chr7.2 CDS 51816366 51816436 51816366 ID=cds.MS.gene23190.t1;Parent=MS.gene23190.t1
chr7.2 exon 51816178 51816244 51816178 ID=MS.gene23190.t1.exon5;Parent=MS.gene23190.t1
chr7.2 CDS 51816178 51816244 51816178 ID=cds.MS.gene23190.t1;Parent=MS.gene23190.t1
chr7.2 exon 51815605 51815676 51815605 ID=MS.gene23190.t1.exon6;Parent=MS.gene23190.t1
chr7.2 CDS 51815605 51815676 51815605 ID=cds.MS.gene23190.t1;Parent=MS.gene23190.t1
chr7.2 exon 51815414 51815473 51815414 ID=MS.gene23190.t1.exon7;Parent=MS.gene23190.t1
chr7.2 CDS 51815414 51815473 51815414 ID=cds.MS.gene23190.t1;Parent=MS.gene23190.t1
Gene Sequence

>MS.gene23190

ATGATAAAGAAGCCACAGGTTGGTAATTTGTCTTGTAATTGATTTTTATCCTATATATGTTGAAAGTTTCTTTCTGTTTAACAATAATTTTCAACTTGCATAGATTGTACTAATAATATGAATTTCTTGCTTTAATCTGTCAGAAGCTCAATGTAATAGTAGGGAACAATGTGGAAATTGGTGCAAATACATGCATTGACAGGGGCAGGTTATTTTTTTGATGTCTTTTATCCTTTTCTATTTAGCTTCATGATGTGTCAAAAAATGTGTTAATGTTTGATTTTTCAGAGCAAAACTGAATCATGAAGATATGAGTGAAAGGAAGAGAAAATTGTATTATGATTAATTAGTTAGAAAATTACAAGAGGTTAAGCTTATATAGCATACACCTATGCCAGCTCATCAGAGTTTCTAACTAACTAAATTCAAGTGAAATTACACTTAAATGCTAATGAAATGTAAATGTGTTAAATGTCTTGTGTCTCTTCTTTAGTTATTATTGTTGATAATTTTCACTTTTAATTGCTATCAACTAATTATATTCACTCATTGGCCATGCAGCTGGAGAGATACATTTATTGGGGACAACTCGAAGATAGATAATTTAGTTCAGGTTAACATTTAGCTTTTACATAATTACAAAAGCGAAAAAAAAAAATGCTTTGTTGTTTTGTTCTGATTAGTTCTGCAGATTGGCCACAATGTAGTTATTGGGAAGAATTGCATGCTATGTGGACAAGTTGGGATTGCAGGCTCAGCAACGTATGTTATCTTCGTTCATTTTGTTGTTATCTAGTAAATTTTGGGATGATACATCTGGTCAAATTCATATTAGAACTAAAGTTATTGCCGAGGCTTACATTTTTCTCTGTTATGTGTTCAGCATAGGAGATTATGTGACGATGGGAGGAAGGGTAGCAGTGCGTGATCATGTATCGATTGCATCAAAGGTATGCTTTTGTTTTACTTGGTGCATTGAGGATGGTTGGCTATTGATTAGCGAATGAATAATTGAACTTTCATGCTTGTTGTAACTTGTAAGACAAACAATATCAAACTATAATGATCTTGGAAAGTGTTATTAAGTTAAAGATTTGAAAGACATTGGGAATAAATACATGTTTTTTTTTTACAAGAGGAAATAAATGCATGTTAATTACCTGAAATTTAGCTCTTTTCATATGCATTTTCTATCATCAGAATAGTTTTCATCTTCACACTCACGGTATCAACACAACCTGTAATTCCTTGCTGGCTGTACTAAGTGCATGTTTGCTTTGGTGGTGAGTTCAGCAGAATCACTGGTGCCACCGTGATTTTGTCATACTCACCGCTATTCAAATGATCAATTGACACTGTTTTAATTTATTTTGCTATTGTATTATTTTTACTGTTTGTAAATTGGGTGCTTGGGGTTGAGCAGAAGATGTCTGATTGCAACGTGCTTGCAGGTTCGACTTGCTGCGTTAAGTTGTGTAACCAAGGACATCACAGAACCTGGAGATTATGGTGGCTTTCCTGCTGTAATGATCTACTAGTCTTGTTATTTATTCAATATAATGCATTAGCATCAAAGTTCATAGTTTGACATTGACATGCATACATGTTATTGGGTTGGGTCCATTCATAAACCAGCTTCCTTGTTGCTTTGTAGGTTTCTATTCACAAATGGCGGAGACAAGTTGCCAGCAGTTTTGCAGTTAGAGGAGTCTGA

Protein sequence

>MS.gene23190.t1

MIKKPQKLNVIVGNNVEIGANTCIDRGSWRDTFIGDNSKIDNLVQIGHNVVIGKNCMLCGQVGIAGSATIGDYVTMGGRVAVRDHVSIASKVRLAALSCVTKDITEPGDYGGFPAVSIHKWRRQVASSFAVRGV