AlfalfaGEDB | MS.gene99849

M. sativa cultivar XinJiangDaYe / MS.gene99849

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99849.t1	KEH33904.1	85	147	15	1	1	140	282	428	1.50E-62	248.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99849.t1	A0A072UVS3	85.0	147	15	1	1	140	282	428	1.1e-62	248.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene99849	0.869944	2.12E-66	-1.69E-46
MS.gene049343	MS.gene99849	0.830152	3.45E-55	-1.69E-46
MS.gene049404	MS.gene99849	0.806918	6.51E-50	-1.69E-46
MS.gene049567	MS.gene99849	0.802515	5.41E-49	-1.69E-46
MS.gene049669	MS.gene99849	0.823732	1.18E-53	-1.69E-46
MS.gene049711	MS.gene99849	0.818441	1.96E-52	-1.69E-46
MS.gene049824	MS.gene99849	0.806793	6.92E-50	-1.69E-46
MS.gene049825	MS.gene99849	0.831608	1.52E-55	-1.69E-46
MS.gene049873	MS.gene99849	0.819256	1.28E-52	-1.69E-46
MS.gene049977	MS.gene99849	0.81434	1.62E-51	-1.69E-46
MS.gene050026	MS.gene99849	0.829773	4.27E-55	-1.69E-46
MS.gene050137	MS.gene99849	0.803892	2.81E-49	-1.69E-46
MS.gene050156	MS.gene99849	0.808572	2.90E-50	-1.69E-46
MS.gene05016	MS.gene99849	0.807599	4.67E-50	-1.69E-46
MS.gene050201	MS.gene99849	0.829968	3.82E-55	-1.69E-46
MS.gene050355	MS.gene99849	0.828201	1.03E-54	-1.69E-46
MS.gene050442	MS.gene99849	0.809033	2.31E-50	-1.69E-46
MS.gene050559	MS.gene99849	0.855311	6.87E-62	-1.69E-46
MS.gene050561	MS.gene99849	0.850307	1.85E-60	-1.69E-46
MS.gene050562	MS.gene99849	0.84457	6.96E-59	-1.69E-46
MS.gene050780	MS.gene99849	0.823948	1.05E-53	-1.69E-46
MS.gene050844	MS.gene99849	0.800505	1.40E-48	-1.69E-46
MS.gene051247	MS.gene99849	0.823215	1.56E-53	-1.69E-46
MS.gene05125	MS.gene99849	0.804294	2.31E-49	-1.69E-46
MS.gene051279	MS.gene99849	0.83189	1.29E-55	-1.69E-46
MS.gene051332	MS.gene99849	0.841425	4.79E-58	-1.69E-46
MS.gene051492	MS.gene99849	0.844246	8.51E-59	-1.69E-46
MS.gene051594	MS.gene99849	0.847517	1.10E-59	-1.69E-46
MS.gene051676	MS.gene99849	0.800283	1.55E-48	-1.69E-46
MS.gene051719	MS.gene99849	0.805386	1.37E-49	-1.69E-46
MS.gene051720	MS.gene99849	0.803449	3.47E-49	-1.69E-46
MS.gene051744	MS.gene99849	0.861682	8.64E-64	-1.69E-46
MS.gene051832	MS.gene99849	0.800113	1.68E-48	-1.69E-46
MS.gene051935	MS.gene99849	0.806798	6.90E-50	-1.69E-46
MS.gene051957	MS.gene99849	0.826666	2.39E-54	-1.69E-46
MS.gene052108	MS.gene99849	0.834786	2.45E-56	-1.69E-46
MS.gene052190	MS.gene99849	0.808685	2.74E-50	-1.69E-46
MS.gene052208	MS.gene99849	0.831396	1.71E-55	-1.69E-46
MS.gene052239	MS.gene99849	-0.814621	1.40E-51	-1.69E-46
MS.gene052333	MS.gene99849	0.825332	4.97E-54	-1.69E-46
MS.gene052358	MS.gene99849	-0.811755	5.97E-51	-1.69E-46
MS.gene052447	MS.gene99849	0.811212	7.84E-51	-1.69E-46
MS.gene052519	MS.gene99849	0.844237	8.56E-59	-1.69E-46
MS.gene052534	MS.gene99849	0.810109	1.36E-50	-1.69E-46
MS.gene052582	MS.gene99849	0.841918	3.55E-58	-1.69E-46
MS.gene052584	MS.gene99849	0.850306	1.85E-60	-1.69E-46
MS.gene052586	MS.gene99849	0.839234	1.79E-57	-1.69E-46
MS.gene052768	MS.gene99849	0.81738	3.40E-52	-1.69E-46
MS.gene052773	MS.gene99849	0.846304	2.36E-59	-1.69E-46
MS.gene052867	MS.gene99849	0.816176	6.33E-52	-1.69E-46
MS.gene053006	MS.gene99849	0.817613	3.01E-52	-1.69E-46
MS.gene053054	MS.gene99849	-0.817288	3.56E-52	-1.69E-46
MS.gene05308	MS.gene99849	0.80692	6.51E-50	-1.69E-46
MS.gene053166	MS.gene99849	0.811158	8.05E-51	-1.69E-46
MS.gene053226	MS.gene99849	0.859668	3.53E-63	-1.69E-46
MS.gene053340	MS.gene99849	0.814018	1.91E-51	-1.69E-46
MS.gene053599	MS.gene99849	0.809179	2.15E-50	-1.69E-46
MS.gene053641	MS.gene99849	0.808427	3.11E-50	-1.69E-46
MS.gene05365	MS.gene99849	0.825817	3.81E-54	-1.69E-46
MS.gene053883	MS.gene99849	0.805471	1.31E-49	-1.69E-46
MS.gene053966	MS.gene99849	0.825394	4.80E-54	-1.69E-46
MS.gene053995	MS.gene99849	0.81287	3.41E-51	-1.69E-46
MS.gene053998	MS.gene99849	0.800051	1.73E-48	-1.69E-46
MS.gene05410	MS.gene99849	0.821695	3.52E-53	-1.69E-46
MS.gene05414	MS.gene99849	0.84349	1.36E-58	-1.69E-46
MS.gene05415	MS.gene99849	0.872688	2.62E-67	-1.69E-46
MS.gene054160	MS.gene99849	0.80871	2.71E-50	-1.69E-46
MS.gene05423	MS.gene99849	0.843087	1.74E-58	-1.69E-46
MS.gene054291	MS.gene99849	-0.850652	1.48E-60	-1.69E-46
MS.gene054468	MS.gene99849	0.829331	5.46E-55	-1.69E-46
MS.gene054479	MS.gene99849	0.802141	6.46E-49	-1.69E-46
MS.gene054576	MS.gene99849	0.850739	1.40E-60	-1.69E-46
MS.gene054651	MS.gene99849	0.845877	3.09E-59	-1.69E-46
MS.gene054775	MS.gene99849	0.82934	5.44E-55	-1.69E-46
MS.gene055166	MS.gene99849	0.827845	1.25E-54	-1.69E-46
MS.gene055228	MS.gene99849	0.819876	9.23E-53	-1.69E-46
MS.gene05531	MS.gene99849	0.804202	2.42E-49	-1.69E-46
MS.gene05548	MS.gene99849	0.804712	1.89E-49	-1.69E-46
MS.gene055586	MS.gene99849	0.8463	2.37E-59	-1.69E-46
MS.gene055589	MS.gene99849	0.832437	9.46E-56	-1.69E-46
MS.gene055778	MS.gene99849	0.847335	1.23E-59	-1.69E-46
MS.gene055818	MS.gene99849	0.842928	1.92E-58	-1.69E-46
MS.gene055841	MS.gene99849	0.821946	3.08E-53	-1.69E-46
MS.gene05604	MS.gene99849	0.822353	2.48E-53	-1.69E-46
MS.gene056050	MS.gene99849	0.8237	1.20E-53	-1.69E-46
MS.gene056091	MS.gene99849	0.806171	9.37E-50	-1.69E-46
MS.gene056162	MS.gene99849	0.839158	1.87E-57	-1.69E-46
MS.gene056174	MS.gene99849	0.815799	7.68E-52	-1.69E-46
MS.gene056272	MS.gene99849	0.814616	1.41E-51	-1.69E-46
MS.gene056366	MS.gene99849	0.841688	4.08E-58	-1.69E-46
MS.gene056373	MS.gene99849	0.805855	1.09E-49	-1.69E-46
MS.gene056506	MS.gene99849	0.814683	1.36E-51	-1.69E-46
MS.gene05659	MS.gene99849	0.811135	8.15E-51	-1.69E-46
MS.gene056776	MS.gene99849	0.828181	1.04E-54	-1.69E-46
MS.gene056779	MS.gene99849	0.816987	4.17E-52	-1.69E-46
MS.gene05686	MS.gene99849	0.816655	4.94E-52	-1.69E-46
MS.gene05690	MS.gene99849	0.810555	1.09E-50	-1.69E-46
MS.gene057012	MS.gene99849	0.801837	7.46E-49	-1.69E-46
MS.gene057026	MS.gene99849	0.861694	8.57E-64	-1.69E-46
MS.gene057027	MS.gene99849	0.812969	3.24E-51	-1.69E-46
MS.gene057060	MS.gene99849	0.831697	1.44E-55	-1.69E-46
MS.gene057102	MS.gene99849	0.813454	2.54E-51	-1.69E-46
MS.gene057208	MS.gene99849	0.854401	1.26E-61	-1.69E-46
MS.gene057414	MS.gene99849	0.815736	7.93E-52	-1.69E-46
MS.gene057444	MS.gene99849	0.818517	1.88E-52	-1.69E-46
MS.gene057598	MS.gene99849	0.824598	7.40E-54	-1.69E-46
MS.gene057611	MS.gene99849	0.811166	8.02E-51	-1.69E-46
MS.gene057693	MS.gene99849	0.815647	8.31E-52	-1.69E-46
MS.gene057921	MS.gene99849	0.800987	1.11E-48	-1.69E-46
MS.gene058008	MS.gene99849	0.804355	2.25E-49	-1.69E-46
MS.gene058037	MS.gene99849	0.823789	1.15E-53	-1.69E-46
MS.gene058477	MS.gene99849	0.815648	8.30E-52	-1.69E-46
MS.gene058625	MS.gene99849	0.848567	5.64E-60	-1.69E-46
MS.gene058636	MS.gene99849	0.811908	5.53E-51	-1.69E-46
MS.gene058829	MS.gene99849	0.817681	2.91E-52	-1.69E-46
MS.gene058979	MS.gene99849	0.844097	9.33E-59	-1.69E-46
MS.gene05897	MS.gene99849	0.801928	7.14E-49	-1.69E-46
MS.gene05925	MS.gene99849	0.808815	2.57E-50	-1.69E-46
MS.gene059439	MS.gene99849	0.800876	1.17E-48	-1.69E-46
MS.gene05950	MS.gene99849	0.807904	4.03E-50	-1.69E-46
MS.gene05954	MS.gene99849	0.81935	1.22E-52	-1.69E-46
MS.gene059584	MS.gene99849	0.813337	2.69E-51	-1.69E-46
MS.gene059625	MS.gene99849	0.850053	2.18E-60	-1.69E-46
MS.gene05985	MS.gene99849	-0.81097	8.84E-51	-1.69E-46
MS.gene059935	MS.gene99849	0.808711	2.71E-50	-1.69E-46
MS.gene060365	MS.gene99849	0.841443	4.74E-58	-1.69E-46
MS.gene060373	MS.gene99849	0.82996	3.84E-55	-1.69E-46
MS.gene060521	MS.gene99849	0.852998	3.19E-61	-1.69E-46
MS.gene060572	MS.gene99849	0.845353	4.28E-59	-1.69E-46
MS.gene060634	MS.gene99849	-0.832915	7.20E-56	-1.69E-46
MS.gene060879	MS.gene99849	0.81234	4.45E-51	-1.69E-46
MS.gene061040	MS.gene99849	-0.830118	3.52E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 20 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAAGCCTTGAAAGAGTTTA+AGG	0.107628	3.1:+39429875	MS.gene99849:CDS
GACTCCCTCTCACAATATTA+AGG	0.347532	3.1:-39429656	None:intergenic
AGGATCCTTAAACTCTTTCA+AGG	0.390271	3.1:-39429880	None:intergenic
AAGAGTTTAAGGATCCTGAT+TGG	0.428920	3.1:+39429886	MS.gene99849:CDS
ACAACTCAGTTTCAAAAGAA+AGG	0.433716	3.1:+39429531	MS.gene99849:CDS
CCTTGACATACACACTTTCG+TGG	0.438856	3.1:-39429757	None:intergenic
ATAAACATTTCTCTCCAATC+AGG	0.454365	3.1:-39429900	None:intergenic
TTATTGCCATGTCTAATGAT+AGG	0.456478	3.1:+39429919	MS.gene99849:CDS
CAACTCAGTTTCAAAAGAAA+GGG	0.496345	3.1:+39429532	MS.gene99849:CDS
AGTGTGTATGTCAAGGGATG+AGG	0.520031	3.1:+39429764	MS.gene99849:CDS
TGTGATCACTATAAGCTGTT+GGG	0.521581	3.1:+39429555	MS.gene99849:CDS
GTGTGATCACTATAAGCTGT+TGG	0.554553	3.1:+39429554	MS.gene99849:CDS
CCACGAAAGTGTGTATGTCA+AGG	0.567251	3.1:+39429757	MS.gene99849:CDS
CACGAAAGTGTGTATGTCAA+GGG	0.572449	3.1:+39429758	MS.gene99849:CDS
CAATACCTTAATATTGTGAG+AGG	0.592433	3.1:+39429651	MS.gene99849:CDS
TTGCCATGTCTAATGATAGG+AGG	0.593182	3.1:+39429922	MS.gene99849:CDS
AATACCTTAATATTGTGAGA+GGG	0.647073	3.1:+39429652	MS.gene99849:CDS
ACGCCTCCTATCATTAGACA+TGG	0.656084	3.1:-39429925	None:intergenic
ATGTCTAATGATAGGAGGCG+TGG	0.662238	3.1:+39429927	MS.gene99849:CDS
TTGTTCTATTACAAAGTGTG+TGG	0.692819	3.1:+39429809	MS.gene99849:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATACCTTAATATTGTGAGA+GGG	+	chr3.1:39429652-39429671	MS.gene99849:CDS	25.0%
!!!	TGGAATTTTGATCTTTCAAT+GGG	-	chr3.1:39429740-39429759	None:intergenic	25.0%
	ACAACTCAGTTTCAAAAGAA+AGG	+	chr3.1:39429531-39429550	MS.gene99849:CDS	30.0%
	ATAAACATTTCTCTCCAATC+AGG	-	chr3.1:39429903-39429922	None:intergenic	30.0%
	CAACTCAGTTTCAAAAGAAA+GGG	+	chr3.1:39429532-39429551	MS.gene99849:CDS	30.0%
	CAATACCTTAATATTGTGAG+AGG	+	chr3.1:39429651-39429670	MS.gene99849:CDS	30.0%
	TTATTGCCATGTCTAATGAT+AGG	+	chr3.1:39429919-39429938	MS.gene99849:CDS	30.0%
	TTGTTCTATTACAAAGTGTG+TGG	+	chr3.1:39429809-39429828	MS.gene99849:CDS	30.0%
!!	GTGGAATTTTGATCTTTCAA+TGG	-	chr3.1:39429741-39429760	None:intergenic	30.0%
	AAGAGTTTAAGGATCCTGAT+TGG	+	chr3.1:39429886-39429905	MS.gene99849:CDS	35.0%
	AGGATCCTTAAACTCTTTCA+AGG	-	chr3.1:39429883-39429902	None:intergenic	35.0%
	GAAAGCCTTGAAAGAGTTTA+AGG	+	chr3.1:39429875-39429894	MS.gene99849:CDS	35.0%
	TGTGATCACTATAAGCTGTT+GGG	+	chr3.1:39429555-39429574	MS.gene99849:CDS	35.0%
	GACTCCCTCTCACAATATTA+AGG	-	chr3.1:39429659-39429678	None:intergenic	40.0%
	GTGTGATCACTATAAGCTGT+TGG	+	chr3.1:39429554-39429573	MS.gene99849:CDS	40.0%
	TTGCCATGTCTAATGATAGG+AGG	+	chr3.1:39429922-39429941	MS.gene99849:CDS	40.0%
!	CACGAAAGTGTGTATGTCAA+GGG	+	chr3.1:39429758-39429777	MS.gene99849:CDS	40.0%
	ACGCCTCCTATCATTAGACA+TGG	-	chr3.1:39429928-39429947	None:intergenic	45.0%
	AGTGTGTATGTCAAGGGATG+AGG	+	chr3.1:39429764-39429783	MS.gene99849:CDS	45.0%
	ATGTCTAATGATAGGAGGCG+TGG	+	chr3.1:39429927-39429946	MS.gene99849:CDS	45.0%
	CCTTGACATACACACTTTCG+TGG	-	chr3.1:39429760-39429779	None:intergenic	45.0%
!	CCACGAAAGTGTGTATGTCA+AGG	+	chr3.1:39429757-39429776	MS.gene99849:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	39429530	39429953	39429530	ID=MS.gene99849
chr3.1	mRNA	39429530	39429953	39429530	ID=MS.gene99849.t1;Parent=MS.gene99849
chr3.1	exon	39429530	39429953	39429530	ID=MS.gene99849.t1.exon1;Parent=MS.gene99849.t1
chr3.1	CDS	39429530	39429953	39429530	ID=cds.MS.gene99849.t1;Parent=MS.gene99849.t1

Gene Sequence

>MS.gene99849

AACAACTCAGTTTCAAAAGAAAGGGTGTGATCACTATAAGCTGTTGGGAATCATATTCAACAACAAAAAAGAAAATGAAGCTTTTCATTACTCATCTAATGAAGAAAATGAGCTTGATAATCAATACCTTAATATTGTGAGAGGGAGTCGTGTACATAATGATATGCAAGAAGTTGAATGTGTCACATGTAGTGTAAAGAGAAAAATCCCATTGAAAGATCAAAATTCCACGAAAGTGTGTATGTCAAGGGATGAGGCTACTTCTTTTGTATCATCAGATTGTTCTATTACAAAGTGTGTGGCTGCTCTTGAAGAGATAGAAGACATTTCAAATGATATTTATCTGAAAGCCTTGAAAGAGTTTAAGGATCCTGATTGGAGAGAAATGTTTATTGCCATGTCTAATGATAGGAGGCGTGGATGA

Protein sequence

>MS.gene99849.t1

NNSVSKERVUSLUAVGNHIQQQKRKUSFSLLIUURKUAUUSIPUYCERESCTUUYARSUMCHMUCKEKNPIERSKFHESVYVKGUGYFFCIIRLFYYKVCGCSURDRRHFKUYLSESLERVUGSULERNVYCHVUUUEAWM