Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37412.t1 | XP_003603170.1 | 100 | 110 | 0 | 0 | 1 | 110 | 1 | 110 | 8.30E-53 | 216.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37412.t1 | G7J8B4 | 100.0 | 110 | 0 | 0 | 1 | 110 | 1 | 110 | 5.9e-53 | 216.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04919 | MS.gene37412 | 0.804735 | 1.87E-49 | -1.69E-46 |
| MS.gene04920 | MS.gene37412 | 0.822771 | 1.98E-53 | -1.69E-46 |
| MS.gene049254 | MS.gene37412 | 0.800678 | 1.29E-48 | -1.69E-46 |
| MS.gene049283 | MS.gene37412 | 0.853176 | 2.84E-61 | -1.69E-46 |
| MS.gene049419 | MS.gene37412 | -0.812412 | 4.30E-51 | -1.69E-46 |
| MS.gene049499 | MS.gene37412 | 0.806657 | 7.40E-50 | -1.69E-46 |
| MS.gene049680 | MS.gene37412 | 0.815129 | 1.08E-51 | -1.69E-46 |
| MS.gene050271 | MS.gene37412 | 0.806777 | 6.98E-50 | -1.69E-46 |
| MS.gene051154 | MS.gene37412 | 0.825153 | 5.47E-54 | -1.69E-46 |
| MS.gene051699 | MS.gene37412 | 0.86254 | 4.71E-64 | -1.69E-46 |
| MS.gene051832 | MS.gene37412 | 0.857175 | 1.95E-62 | -1.69E-46 |
| MS.gene052118 | MS.gene37412 | 0.80335 | 3.64E-49 | -1.69E-46 |
| MS.gene052533 | MS.gene37412 | 0.810538 | 1.10E-50 | -1.69E-46 |
| MS.gene052559 | MS.gene37412 | 0.821592 | 3.72E-53 | -1.69E-46 |
| MS.gene052685 | MS.gene37412 | 0.821022 | 5.04E-53 | -1.69E-46 |
| MS.gene052686 | MS.gene37412 | 0.812637 | 3.84E-51 | -1.69E-46 |
| MS.gene052793 | MS.gene37412 | 0.805804 | 1.12E-49 | -1.69E-46 |
| MS.gene053094 | MS.gene37412 | 0.820823 | 5.60E-53 | -1.69E-46 |
| MS.gene053298 | MS.gene37412 | 0.819284 | 1.26E-52 | -1.69E-46 |
| MS.gene053340 | MS.gene37412 | 0.827617 | 1.42E-54 | -1.69E-46 |
| MS.gene053963 | MS.gene37412 | 0.894724 | 1.88E-75 | -1.69E-46 |
| MS.gene054222 | MS.gene37412 | 0.860907 | 1.49E-63 | -1.69E-46 |
| MS.gene054490 | MS.gene37412 | 0.808623 | 2.83E-50 | -1.69E-46 |
| MS.gene054684 | MS.gene37412 | 0.82145 | 4.01E-53 | -1.69E-46 |
| MS.gene054892 | MS.gene37412 | 0.835176 | 1.95E-56 | -1.69E-46 |
| MS.gene054893 | MS.gene37412 | 0.836366 | 9.76E-57 | -1.69E-46 |
| MS.gene054957 | MS.gene37412 | 0.840233 | 9.84E-58 | -1.69E-46 |
| MS.gene055147 | MS.gene37412 | 0.852194 | 5.42E-61 | -1.69E-46 |
| MS.gene055148 | MS.gene37412 | 0.845749 | 3.34E-59 | -1.69E-46 |
| MS.gene055149 | MS.gene37412 | 0.814913 | 1.21E-51 | -1.69E-46 |
| MS.gene055150 | MS.gene37412 | 0.83773 | 4.38E-57 | -1.69E-46 |
| MS.gene055226 | MS.gene37412 | 0.806398 | 8.39E-50 | -1.69E-46 |
| MS.gene055229 | MS.gene37412 | 0.830365 | 3.06E-55 | -1.69E-46 |
| MS.gene056315 | MS.gene37412 | 0.801331 | 9.47E-49 | -1.69E-46 |
| MS.gene05636 | MS.gene37412 | 0.801212 | 1.00E-48 | -1.69E-46 |
| MS.gene056677 | MS.gene37412 | 0.810152 | 1.33E-50 | -1.69E-46 |
| MS.gene056986 | MS.gene37412 | 0.802847 | 4.62E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene37412 | 0.83248 | 9.23E-56 | -1.69E-46 |
| MS.gene057208 | MS.gene37412 | 0.812052 | 5.15E-51 | -1.69E-46 |
| MS.gene057622 | MS.gene37412 | 0.806302 | 8.79E-50 | -1.69E-46 |
| MS.gene058159 | MS.gene37412 | 0.829903 | 3.97E-55 | -1.69E-46 |
| MS.gene058279 | MS.gene37412 | 0.804189 | 2.43E-49 | -1.69E-46 |
| MS.gene058592 | MS.gene37412 | 0.852527 | 4.35E-61 | -1.69E-46 |
| MS.gene05878 | MS.gene37412 | 0.816649 | 4.96E-52 | -1.69E-46 |
| MS.gene05952 | MS.gene37412 | 0.882525 | 9.69E-71 | -1.69E-46 |
| MS.gene05956 | MS.gene37412 | 0.812202 | 4.77E-51 | -1.69E-46 |
| MS.gene059774 | MS.gene37412 | 0.800788 | 1.22E-48 | -1.69E-46 |
| MS.gene05985 | MS.gene37412 | -0.875017 | 4.29E-68 | -1.69E-46 |
| MS.gene059901 | MS.gene37412 | -0.80011 | 1.68E-48 | -1.69E-46 |
| MS.gene060012 | MS.gene37412 | 0.813393 | 2.62E-51 | -1.69E-46 |
| MS.gene06023 | MS.gene37412 | 0.813701 | 2.24E-51 | -1.69E-46 |
| MS.gene060412 | MS.gene37412 | 0.841845 | 3.71E-58 | -1.69E-46 |
| MS.gene060840 | MS.gene37412 | 0.814886 | 1.23E-51 | -1.69E-46 |
| MS.gene060879 | MS.gene37412 | 0.801415 | 9.10E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene37412 | 0.815059 | 1.12E-51 | -1.69E-46 |
| MS.gene060937 | MS.gene37412 | 0.832169 | 1.10E-55 | -1.69E-46 |
| MS.gene061047 | MS.gene37412 | 0.810684 | 1.02E-50 | -1.69E-46 |
| MS.gene061188 | MS.gene37412 | 0.823492 | 1.35E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37412.t1 | MTR_3g104640 | 100.000 | 110 | 0 | 0 | 1 | 110 | 1 | 110 | 1.63e-74 | 216 |
| MS.gene37412.t1 | MTR_4g125990 | 55.906 | 127 | 35 | 5 | 1 | 110 | 1 | 123 | 8.48e-35 | 116 |
| MS.gene37412.t1 | MTR_1g015810 | 67.241 | 116 | 30 | 5 | 1 | 108 | 1 | 116 | 6.41e-32 | 108 |
| MS.gene37412.t1 | MTR_2g099630 | 49.541 | 109 | 44 | 4 | 6 | 109 | 4 | 106 | 4.69e-26 | 93.6 |
| MS.gene37412.t1 | MTR_8g018320 | 50.495 | 101 | 49 | 1 | 6 | 106 | 4 | 103 | 6.89e-22 | 83.2 |
| MS.gene37412.t1 | MTR_8g018360 | 50.000 | 102 | 50 | 1 | 6 | 107 | 4 | 104 | 7.16e-21 | 82.8 |
| MS.gene37412.t1 | MTR_7g109290 | 46.667 | 60 | 32 | 0 | 51 | 110 | 32 | 91 | 5.29e-14 | 62.4 |
| MS.gene37412.t1 | MTR_1g098310 | 57.407 | 54 | 20 | 1 | 56 | 109 | 35 | 85 | 2.54e-13 | 60.8 |
| MS.gene37412.t1 | MTR_1g087730 | 42.373 | 59 | 34 | 0 | 52 | 110 | 48 | 106 | 9.62e-13 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37412.t1 | AT5G11970 | 59.091 | 110 | 39 | 3 | 1 | 110 | 1 | 104 | 1.79e-35 | 117 |
| MS.gene37412.t1 | AT2G19460 | 45.455 | 121 | 46 | 5 | 1 | 107 | 1 | 115 | 4.13e-21 | 81.6 |
| MS.gene37412.t1 | AT2G19460 | 45.455 | 121 | 46 | 5 | 1 | 107 | 53 | 167 | 1.95e-20 | 81.3 |
| MS.gene37412.t1 | AT2G47480 | 51.613 | 62 | 27 | 1 | 50 | 108 | 47 | 108 | 5.75e-15 | 65.5 |
| MS.gene37412.t1 | AT3G13910 | 58.621 | 58 | 23 | 1 | 45 | 101 | 40 | 97 | 2.48e-14 | 63.9 |
| MS.gene37412.t1 | AT3G13910 | 58.621 | 58 | 23 | 1 | 45 | 101 | 40 | 97 | 5.38e-14 | 63.2 |
| MS.gene37412.t1 | AT1G72720 | 62.222 | 45 | 17 | 0 | 65 | 109 | 81 | 125 | 6.28e-14 | 63.5 |
| MS.gene37412.t1 | AT3G62640 | 51.111 | 45 | 22 | 0 | 65 | 109 | 66 | 110 | 6.18e-13 | 60.5 |
| MS.gene37412.t1 | AT3G05725 | 35.115 | 131 | 55 | 7 | 1 | 109 | 1 | 123 | 1.61e-12 | 59.7 |
| MS.gene37412.t1 | AT4G09890 | 53.846 | 39 | 18 | 0 | 66 | 104 | 39 | 77 | 5.58e-11 | 54.7 |
Find 21 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTACTCACAGGTGGTTTA+TGG | 0.228498 | 3.4:-91630129 | MS.gene37412:CDS |
| CTCTTCTTCCTCTGAAGTTC+AGG | 0.289847 | 3.4:+91630231 | None:intergenic |
| AGCTTTCTATCTGCATCATT+TGG | 0.373432 | 3.4:+91630394 | None:intergenic |
| TATAAAATGTATTCTGTTGA+AGG | 0.385236 | 3.4:-91630198 | MS.gene37412:CDS |
| CTAAGATCATAAGGCTTAGA+TGG | 0.404348 | 3.4:+91630363 | None:intergenic |
| ATGCAGATAGAAAGCTACTA+TGG | 0.407129 | 3.4:-91630387 | MS.gene37412:CDS |
| TATCTGCATCATTTGGCCAT+TGG | 0.440208 | 3.4:+91630401 | None:intergenic |
| CTATAGCTTCTAAGATCATA+AGG | 0.444134 | 3.4:+91630354 | None:intergenic |
| CTGAACTTCAGAGGAAGAAG+AGG | 0.478478 | 3.4:-91630230 | MS.gene37412:CDS |
| TTCTTCAACTTCAAATCCTT+AGG | 0.481282 | 3.4:+91630309 | None:intergenic |
| AAGGATTTGAAGTTGAAGAA+AGG | 0.511800 | 3.4:-91630306 | MS.gene37412:CDS |
| CTTAGGAGTTTGTTGTACAT+AGG | 0.522202 | 3.4:+91630326 | None:intergenic |
| CTATGTACAACAAACTCCTA+AGG | 0.555597 | 3.4:-91630325 | MS.gene37412:CDS |
| TCTGTTGAAGGCAAAGTCAA+AGG | 0.571369 | 3.4:-91630186 | MS.gene37412:CDS |
| CTTTCAGAAAGAGTTTCAGA+TGG | 0.576426 | 3.4:-91630161 | MS.gene37412:CDS |
| ACTCACAGGTGGTTTATGGT+TGG | 0.580184 | 3.4:-91630125 | MS.gene37412:CDS |
| TGTCTGATCCTGAACTTCAG+AGG | 0.591643 | 3.4:-91630239 | MS.gene37412:CDS |
| AGATCAAAATCTTATGCCAA+TGG | 0.604385 | 3.4:-91630417 | MS.gene37412:CDS |
| GCTTAAGAACAAGTACTCAC+AGG | 0.640169 | 3.4:-91630139 | MS.gene37412:CDS |
| TGAACTTCAGAGGAAGAAGA+GGG | 0.657165 | 3.4:-91630229 | MS.gene37412:CDS |
| TAAGAACAAGTACTCACAGG+TGG | 0.685797 | 3.4:-91630136 | MS.gene37412:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAAAATGTATTCTGTTGA+AGG | - | chr3.4:91630356-91630375 | MS.gene37412:CDS | 20.0% |
| AGATCAAAATCTTATGCCAA+TGG | - | chr3.4:91630137-91630156 | MS.gene37412:CDS | 30.0% | |
| TTCTTCAACTTCAAATCCTT+AGG | + | chr3.4:91630248-91630267 | None:intergenic | 30.0% | |
| ! | AAAGGTAAAAGCTTTTCATC+TGG | - | chr3.4:91630266-91630285 | MS.gene37412:CDS | 30.0% |
| ! | AAGGATTTGAAGTTGAAGAA+AGG | - | chr3.4:91630248-91630267 | MS.gene37412:CDS | 30.0% |
| !! | CTATAGCTTCTAAGATCATA+AGG | + | chr3.4:91630203-91630222 | None:intergenic | 30.0% |
| AGCTTTCTATCTGCATCATT+TGG | + | chr3.4:91630163-91630182 | None:intergenic | 35.0% | |
| ATGCAGATAGAAAGCTACTA+TGG | - | chr3.4:91630167-91630186 | MS.gene37412:CDS | 35.0% | |
| CTAAGATCATAAGGCTTAGA+TGG | + | chr3.4:91630194-91630213 | None:intergenic | 35.0% | |
| CTATGTACAACAAACTCCTA+AGG | - | chr3.4:91630229-91630248 | MS.gene37412:CDS | 35.0% | |
| CTTTCAGAAAGAGTTTCAGA+TGG | - | chr3.4:91630393-91630412 | MS.gene37412:CDS | 35.0% | |
| !! | CTTAGGAGTTTGTTGTACAT+AGG | + | chr3.4:91630231-91630250 | None:intergenic | 35.0% |
| AAGTACTCACAGGTGGTTTA+TGG | - | chr3.4:91630425-91630444 | MS.gene37412:CDS | 40.0% | |
| GCTTAAGAACAAGTACTCAC+AGG | - | chr3.4:91630415-91630434 | MS.gene37412:CDS | 40.0% | |
| TAAGAACAAGTACTCACAGG+TGG | - | chr3.4:91630418-91630437 | MS.gene37412:CDS | 40.0% | |
| TATCTGCATCATTTGGCCAT+TGG | + | chr3.4:91630156-91630175 | None:intergenic | 40.0% | |
| TCTGTTGAAGGCAAAGTCAA+AGG | - | chr3.4:91630368-91630387 | MS.gene37412:CDS | 40.0% | |
| TGAACTTCAGAGGAAGAAGA+GGG | - | chr3.4:91630325-91630344 | MS.gene37412:CDS | 40.0% | |
| ACTCACAGGTGGTTTATGGT+TGG | - | chr3.4:91630429-91630448 | MS.gene37412:CDS | 45.0% | |
| CTGAACTTCAGAGGAAGAAG+AGG | - | chr3.4:91630324-91630343 | MS.gene37412:CDS | 45.0% | |
| TGTCTGATCCTGAACTTCAG+AGG | - | chr3.4:91630315-91630334 | MS.gene37412:CDS | 45.0% | |
| !! | CTCTTCTTCCTCTGAAGTTC+AGG | + | chr3.4:91630326-91630345 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 91630122 | 91630454 | 91630122 | ID=MS.gene37412 |
| chr3.4 | mRNA | 91630122 | 91630454 | 91630122 | ID=MS.gene37412.t1;Parent=MS.gene37412 |
| chr3.4 | exon | 91630122 | 91630454 | 91630122 | ID=MS.gene37412.t1.exon1;Parent=MS.gene37412.t1 |
| chr3.4 | CDS | 91630122 | 91630454 | 91630122 | ID=cds.MS.gene37412.t1;Parent=MS.gene37412.t1 |
| Gene Sequence |
| Protein sequence |
