AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59903


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59903.t1 MTR_4g113070 99.270 137 1 0 1 137 1245 1381 6.36e-92 290
MS.gene59903.t1 MTR_2g102660 62.205 127 48 0 1 127 1256 1382 3.80e-53 179
MS.gene59903.t1 MTR_2g102670 61.417 127 49 0 1 127 1239 1365 5.44e-53 179
MS.gene59903.t1 MTR_2g102640 61.417 127 49 0 1 127 1259 1385 2.09e-52 177
MS.gene59903.t1 MTR_4g011640 61.600 125 48 0 1 125 1264 1388 3.64e-52 177
MS.gene59903.t1 MTR_4g011630 60.000 125 50 0 1 125 1267 1391 1.88e-50 172
MS.gene59903.t1 MTR_7g098740 57.937 126 53 0 1 126 1242 1367 6.88e-50 170
MS.gene59903.t1 MTR_1g011650 59.055 127 50 1 1 127 1278 1402 1.09e-48 167
MS.gene59903.t1 MTR_7g098750 56.693 127 55 0 1 127 1284 1410 1.18e-48 167
MS.gene59903.t1 MTR_7g098370 57.143 126 54 0 1 126 1242 1367 1.82e-48 166
MS.gene59903.t1 MTR_7g098750 57.143 126 54 0 1 126 1014 1139 1.87e-48 166
MS.gene59903.t1 MTR_7g098760 54.839 124 56 0 1 124 1281 1404 9.37e-48 164
MS.gene59903.t1 MTR_1g492950 51.111 135 66 0 1 135 1295 1429 2.73e-44 154
MS.gene59903.t1 MTR_1g011640 55.469 128 55 1 1 128 1255 1380 5.42e-44 153
MS.gene59903.t1 MTR_7g098780 49.635 137 68 1 1 137 1246 1381 1.59e-42 149
MS.gene59903.t1 MTR_4g011620 57.724 123 52 0 1 123 1257 1379 4.35e-41 145
MS.gene59903.t1 MTR_7g098320 54.032 124 57 0 1 124 1276 1399 6.86e-41 144
MS.gene59903.t1 MTR_1g050525 54.545 121 55 0 1 121 1295 1415 3.43e-40 142
MS.gene59903.t1 MTR_7g104130 51.111 135 66 0 1 135 1284 1418 1.45e-39 140
MS.gene59903.t1 MTR_2g101090 56.250 128 50 1 1 128 1272 1393 3.03e-39 140
MS.gene59903.t1 MTR_7g098890 49.635 137 68 1 1 137 1184 1319 9.48e-37 133
MS.gene59903.t1 MTR_7g104150 45.185 135 74 0 1 135 1284 1418 1.21e-36 132
MS.gene59903.t1 MTR_7g098300 52.033 123 59 0 4 126 1279 1401 1.99e-36 132
MS.gene59903.t1 MTR_7g098800 51.095 137 66 1 1 137 1204 1339 6.85e-36 130
MS.gene59903.t1 MTR_7g407080 56.436 101 44 0 1 101 480 580 9.36e-36 129
MS.gene59903.t1 MTR_7g104110 45.926 135 73 0 1 135 1276 1410 2.00e-35 129
MS.gene59903.t1 MTR_8g014360 54.545 121 55 0 1 121 1306 1426 2.06e-35 129
MS.gene59903.t1 MTR_7g104100 40.876 137 81 0 1 137 1282 1418 2.60e-34 125
MS.gene59903.t1 MTR_4g123850 45.185 135 73 1 1 135 1259 1392 4.43e-34 125
MS.gene59903.t1 MTR_3g463680 47.899 119 62 0 2 120 1245 1363 5.87e-34 125
MS.gene59903.t1 MTR_3g107870 51.181 127 60 1 1 127 1291 1415 3.43e-31 117
MS.gene59903.t1 MTR_7g098750 55.952 84 37 0 1 84 1284 1367 7.29e-28 107
MS.gene59903.t1 MTR_5g070320 50.442 113 56 0 2 114 1260 1372 1.40e-27 107
MS.gene59903.t1 MTR_5g070320 50.442 113 56 0 2 114 973 1085 1.97e-27 106
MS.gene59903.t1 MTR_5g070320 50.442 113 56 0 2 114 1069 1181 2.02e-27 106
MS.gene59903.t1 MTR_7g104130 48.810 84 43 0 1 84 1284 1367 1.03e-17 78.6
MS.gene59903.t1 MTR_7g098790 36.782 87 50 3 55 137 194 279 5.01e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59903.t1 AT2G26910 70.073 137 40 1 1 136 1246 1382 6.20e-65 213
MS.gene59903.t1 AT1G15520 60.526 114 45 0 1 114 1253 1366 5.82e-45 156
MS.gene59903.t1 AT1G66950 49.635 137 69 0 1 137 1281 1417 3.34e-41 145
MS.gene59903.t1 AT3G16340 52.344 128 61 0 1 128 1242 1369 2.71e-40 143
MS.gene59903.t1 AT3G16340 52.344 128 61 0 1 128 1237 1364 2.79e-40 143
MS.gene59903.t1 AT2G36380 48.905 137 70 0 1 137 1280 1416 2.06e-39 140
MS.gene59903.t1 AT1G15210 52.000 125 60 0 1 125 1268 1392 3.78e-39 140
MS.gene59903.t1 AT1G59870 49.600 125 63 0 1 125 1295 1419 4.19e-38 137
MS.gene59903.t1 AT2G29940 47.899 119 62 0 2 120 1255 1373 1.69e-34 126
MS.gene59903.t1 AT4G15230 37.226 137 85 1 1 137 1228 1363 8.72e-29 110
MS.gene59903.t1 AT4G15230 37.226 137 85 1 1 137 1228 1363 8.72e-29 110
MS.gene59903.t1 AT3G30842 40.945 127 73 1 1 127 1249 1373 1.16e-28 110
MS.gene59903.t1 AT4G15233 41.176 119 70 0 1 119 1077 1195 3.44e-27 105
MS.gene59903.t1 AT4G15233 41.176 119 70 0 1 119 1077 1195 3.44e-27 105
MS.gene59903.t1 AT4G15233 41.176 119 70 0 1 119 1158 1276 3.49e-27 105
MS.gene59903.t1 AT4G15233 41.176 119 70 0 1 119 1158 1276 3.49e-27 105
MS.gene59903.t1 AT4G15233 41.176 119 70 0 1 119 1202 1320 3.89e-27 105
MS.gene59903.t1 AT4G15233 41.176 119 70 0 1 119 1211 1329 4.01e-27 105
MS.gene59903.t1 AT4G15233 41.176 119 70 0 1 119 1220 1338 4.42e-27 105
MS.gene59903.t1 AT4G15236 38.686 137 83 1 1 137 1218 1353 5.91e-27 105
MS.gene59903.t1 AT4G15230 39.496 119 72 0 1 119 1149 1267 1.36e-26 103
MS.gene59903.t1 AT4G15215 37.815 119 74 0 1 119 1215 1333 1.25e-25 101
MS.gene59903.t1 AT4G15215 37.815 119 74 0 1 119 1215 1333 1.25e-25 101
MS.gene59903.t1 AT4G15215 37.815 119 74 0 1 119 1215 1333 1.25e-25 101
MS.gene59903.t1 AT4G15215 37.815 119 74 0 1 119 1215 1333 1.25e-25 101
MS.gene59903.t1 AT4G15215 37.815 119 74 0 1 119 1222 1340 1.28e-25 101
MS.gene59903.t1 AT4G15215 37.815 119 74 0 1 119 1222 1340 1.28e-25 101
MS.gene59903.t1 AT4G15215 37.815 119 74 0 1 119 1222 1340 1.28e-25 101
MS.gene59903.t1 AT4G15215 37.815 119 74 0 1 119 1222 1340 1.28e-25 101
MS.gene59903.t1 AT4G15215 37.815 119 74 0 1 119 1222 1340 1.28e-25 101
MS.gene59903.t1 AT4G15215 37.815 119 74 0 1 119 1218 1336 1.30e-25 101
MS.gene59903.t1 AT4G15215 37.815 119 74 0 1 119 1225 1343 1.30e-25 101
MS.gene59903.t1 AT3G53480 38.686 137 83 1 1 137 1278 1413 3.00e-25 100
MS.gene59903.t1 AT2G37280 38.596 114 70 0 1 114 1083 1196 4.37e-23 94.0
MS.gene59903.t1 AT2G37280 38.596 114 70 0 1 114 1270 1383 4.45e-23 94.0
MS.gene59903.t1 AT2G37280 38.596 114 70 0 1 114 1241 1354 4.95e-23 94.0
MS.gene59903.t1 AT4G15233 34.266 143 70 1 1 119 1220 1362 5.23e-23 94.0
MS.gene59903.t1 AT4G15230 33.333 90 59 1 1 89 1228 1317 1.78e-13 66.6
MS.gene59903.t1 AT2G37280 34.524 84 55 0 1 84 1241 1324 6.73e-11 59.3

Find 35 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTTGAGTACGAGTCTTA+TGG 0.249406 8.4:+15648159 None:intergenic
ATTTACAGAGAATCCCTATT+TGG 0.269913 8.4:-15648294 MS.gene59903:intron
CATTTGTCTGGACTGTTGAT+AGG 0.287754 8.4:-15648639 MS.gene59903:CDS
TAATACCATCTCCACCAAAT+AGG 0.298789 8.4:+15648280 None:intergenic
ATTCCATGGGTTCATTTGTC+TGG 0.300093 8.4:-15648651 MS.gene59903:CDS
TTTGGTGGAGATGGTATTAC+TGG 0.315311 8.4:-15648276 MS.gene59903:CDS
TCTTTGAGTACGAGTCTTAT+GGG 0.344416 8.4:+15648160 None:intergenic
AATACCATCTCCACCAAATA+GGG 0.403081 8.4:+15648281 None:intergenic
CGTCACCATACTGTGAAGTT+AGG 0.409814 8.4:+15648221 None:intergenic
GGACTGTTGATAGGTTCATT+TGG 0.416665 8.4:-15648630 MS.gene59903:CDS
ATGGCAGCAACATGATGATT+TGG 0.431081 8.4:+15648538 None:intergenic
AGTAGTCATCATCCCATAGA+AGG 0.438282 8.4:+15648570 None:intergenic
CCATACAAAGTCCAGGCTAC+AGG 0.440044 8.4:+15648247 None:intergenic
CCTGTAGCCTGGACTTTGTA+TGG 0.447509 8.4:-15648247 MS.gene59903:CDS
ATCAGTTCCCATACCTTATG+AGG 0.447971 8.4:+15648463 None:intergenic
ATAGCCTGTGCAAACACATA+AGG 0.448636 8.4:+15648694 None:intergenic
GGTCTCCTAACTTCACAGTA+TGG 0.458953 8.4:-15648226 MS.gene59903:CDS
GAATCATAAAACCACTGAAA+AGG 0.509394 8.4:+15648485 None:intergenic
GAATCCCTATTTGGTGGAGA+TGG 0.512403 8.4:-15648285 MS.gene59903:CDS
GTTCCCTTATGTGTTTGCAC+AGG 0.515664 8.4:-15648698 MS.gene59903:CDS
CATCATGTTGCTGCCATCAT+CGG 0.523643 8.4:-15648532 MS.gene59903:CDS
TAGCCTGTGCAAACACATAA+GGG 0.525322 8.4:+15648695 None:intergenic
TATGGGTACCGAGCTTCCAT+TGG 0.528031 8.4:+15648177 None:intergenic
ACTGGGCAAACCCTGTAGCC+TGG 0.532642 8.4:-15648258 MS.gene59903:CDS
TACAGAGAATCCCTATTTGG+TGG 0.545065 8.4:-15648291 MS.gene59903:intron
ATAACATGGAGCACCGATGA+TGG 0.548775 8.4:+15648519 None:intergenic
TAGGAGACCATACAAAGTCC+AGG 0.559858 8.4:+15648240 None:intergenic
CATACAAAGTCCAGGCTACA+GGG 0.571123 8.4:+15648248 None:intergenic
GTGCTCCATGTTATATGTTG+TGG 0.581246 8.4:-15648510 MS.gene59903:CDS
GAAGCTAACCAATGGAAGCT+CGG 0.609079 8.4:-15648185 MS.gene59903:CDS
GTATGGTGACGATGATAAGT+TGG 0.618130 8.4:-15648209 MS.gene59903:CDS
AGTCCAGACAAATGAACCCA+TGG 0.630269 8.4:+15648648 None:intergenic
AAGTTGGTGAAGCTAACCAA+TGG 0.632225 8.4:-15648193 MS.gene59903:CDS
TTGGTGGAGATGGTATTACT+GGG 0.639389 8.4:-15648275 MS.gene59903:CDS
AGGTTCCACAACATATAACA+TGG 0.641887 8.4:+15648505 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTTATATTTTACCTTCTA+TGG - chr8.4:15648277-15648296 MS.gene59903:CDS 20.0%
!!! TGTTATATTTTACCTTCTAT+GGG - chr8.4:15648278-15648297 MS.gene59903:CDS 20.0%
!! CTCTCATAACAATAACTTTT+TGG - chr8.4:15648451-15648470 MS.gene59903:intron 25.0%
!! TCTTCAATTTTCTATTCCAT+GGG - chr8.4:15648196-15648215 MS.gene59903:CDS 25.0%
ATTTACAGAGAATCCCTATT+TGG - chr8.4:15648566-15648585 MS.gene59903:CDS 30.0%
GAATCATAAAACCACTGAAA+AGG + chr8.4:15648378-15648397 None:intergenic 30.0%
TATGATTCCTCATAAGGTAT+GGG - chr8.4:15648390-15648409 MS.gene59903:intron 30.0%
TTATGATTCCTCATAAGGTA+TGG - chr8.4:15648389-15648408 MS.gene59903:intron 30.0%
! CTCTTCAATTTTCTATTCCA+TGG - chr8.4:15648195-15648214 MS.gene59903:CDS 30.0%
! GATGATAATTTTCGACATCA+AGG + chr8.4:15648481-15648500 None:intergenic 30.0%
! TCGTACTCAAAGAAGTTTTT+GGG - chr8.4:15648710-15648729 MS.gene59903:CDS 30.0%
!!! TGGTTTTATGATTCCTCATA+AGG - chr8.4:15648384-15648403 MS.gene59903:intron 30.0%
AATACCATCTCCACCAAATA+GGG + chr8.4:15648582-15648601 None:intergenic 35.0%
AGGTTCCACAACATATAACA+TGG + chr8.4:15648358-15648377 None:intergenic 35.0%
GGGATTCTCTGTAAATTTCA+AGG + chr8.4:15648562-15648581 None:intergenic 35.0%
GTTGCTTCCATTTACAGTTT+CGG - chr8.4:15648425-15648444 MS.gene59903:intron 35.0%
TAATACCATCTCCACCAAAT+AGG + chr8.4:15648583-15648602 None:intergenic 35.0%
TTCTTTGAGTACGAGTCTTA+TGG + chr8.4:15648704-15648723 None:intergenic 35.0%
! CTCGTACTCAAAGAAGTTTT+TGG - chr8.4:15648709-15648728 MS.gene59903:CDS 35.0%
! TCTTTGAGTACGAGTCTTAT+GGG + chr8.4:15648703-15648722 None:intergenic 35.0%
AGAGCAACCGAAACTGTAAA+TGG + chr8.4:15648435-15648454 None:intergenic 40.0%
AGTAGTCATCATCCCATAGA+AGG + chr8.4:15648293-15648312 None:intergenic 40.0%
ATAGCCTGTGCAAACACATA+AGG + chr8.4:15648169-15648188 None:intergenic 40.0%
ATCAGTTCCCATACCTTATG+AGG + chr8.4:15648400-15648419 None:intergenic 40.0%
ATGGCAGCAACATGATGATT+TGG + chr8.4:15648325-15648344 None:intergenic 40.0%
ATTCCATGGGTTCATTTGTC+TGG - chr8.4:15648209-15648228 MS.gene59903:CDS 40.0%
CATTTGTCTGGACTGTTGAT+AGG - chr8.4:15648221-15648240 MS.gene59903:CDS 40.0%
GTGCTCCATGTTATATGTTG+TGG - chr8.4:15648350-15648369 MS.gene59903:intron 40.0%
TACAGAGAATCCCTATTTGG+TGG - chr8.4:15648569-15648588 MS.gene59903:CDS 40.0%
TAGCCTGTGCAAACACATAA+GGG + chr8.4:15648168-15648187 None:intergenic 40.0%
TTGGTGGAGATGGTATTACT+GGG - chr8.4:15648585-15648604 MS.gene59903:CDS 40.0%
TTTGGTGGAGATGGTATTAC+TGG - chr8.4:15648584-15648603 MS.gene59903:CDS 40.0%
! ATGTTGTGGAACCTTTTCAG+TGG - chr8.4:15648364-15648383 MS.gene59903:intron 40.0%
! GGACTGTTGATAGGTTCATT+TGG - chr8.4:15648230-15648249 MS.gene59903:CDS 40.0%
! GTATGGTGACGATGATAAGT+TGG - chr8.4:15648651-15648670 MS.gene59903:CDS 40.0%
!! AAGTTGGTGAAGCTAACCAA+TGG - chr8.4:15648667-15648686 MS.gene59903:CDS 40.0%
AGTCCAGACAAATGAACCCA+TGG + chr8.4:15648215-15648234 None:intergenic 45.0%
CATACAAAGTCCAGGCTACA+GGG + chr8.4:15648615-15648634 None:intergenic 45.0%
CATCATGTTGCTGCCATCAT+CGG - chr8.4:15648328-15648347 MS.gene59903:intron 45.0%
CGTCACCATACTGTGAAGTT+AGG + chr8.4:15648642-15648661 None:intergenic 45.0%
GAAGCTAACCAATGGAAGCT+CGG - chr8.4:15648675-15648694 MS.gene59903:CDS 45.0%
GGTCTCCTAACTTCACAGTA+TGG - chr8.4:15648634-15648653 MS.gene59903:CDS 45.0%
GTTCCCTTATGTGTTTGCAC+AGG - chr8.4:15648162-15648181 MS.gene59903:CDS 45.0%
TAGGAGACCATACAAAGTCC+AGG + chr8.4:15648623-15648642 None:intergenic 45.0%
! GAATCCCTATTTGGTGGAGA+TGG - chr8.4:15648575-15648594 MS.gene59903:CDS 45.0%
!! ATAACATGGAGCACCGATGA+TGG + chr8.4:15648344-15648363 None:intergenic 45.0%
CCATACAAAGTCCAGGCTAC+AGG + chr8.4:15648616-15648635 None:intergenic 50.0%
CCTGTAGCCTGGACTTTGTA+TGG - chr8.4:15648613-15648632 MS.gene59903:CDS 50.0%
!! TATGGGTACCGAGCTTCCAT+TGG + chr8.4:15648686-15648705 None:intergenic 50.0%
ACTGGGCAAACCCTGTAGCC+TGG - chr8.4:15648602-15648621 MS.gene59903:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 15648151 15648731 15648151 ID=MS.gene59903
chr8.4 mRNA 15648151 15648731 15648151 ID=MS.gene59903.t1;Parent=MS.gene59903
chr8.4 exon 15648477 15648731 15648477 ID=MS.gene59903.t1.exon1;Parent=MS.gene59903.t1
chr8.4 CDS 15648477 15648731 15648477 ID=cds.MS.gene59903.t1;Parent=MS.gene59903.t1
chr8.4 exon 15648151 15648308 15648151 ID=MS.gene59903.t1.exon2;Parent=MS.gene59903.t1
chr8.4 CDS 15648151 15648308 15648151 ID=cds.MS.gene59903.t1;Parent=MS.gene59903.t1
Gene Sequence

>MS.gene59903

GTTGTGATTGAGTTCCCTTATGTGTTTGCACAGGCTATCATATACTCTTCAATTTTCTATTCCATGGGTTCATTTGTCTGGACTGTTGATAGGTTCATTTGGTACTTATTCTTCATGTATTTGACTATGTTATATTTTACCTTCTATGGGATGATGACTACTGCTGTTACACCAAATCATCATGTTGCTGCCATCATCGGTGCTCCATGTTATATGTTGTGGAACCTTTTCAGTGGTTTTATGATTCCTCATAAGGTATGGGAACTGATGCTTTGTTGCTTCCATTTACAGTTTCGGTTGCTCTCATAACAATAACTTTTTGGACTACCTTGATGTCGAAAATTATCATCTAGTATTATACTGATGTAATGTTTTTGAGAATTATCTGTCATCGAATAACTTATTTCTCCTTGAAATTTACAGAGAATCCCTATTTGGTGGAGATGGTATTACTGGGCAAACCCTGTAGCCTGGACTTTGTATGGTCTCCTAACTTCACAGTATGGTGACGATGATAAGTTGGTGAAGCTAACCAATGGAAGCTCGGTACCCATAAGACTCGTACTCAAAGAAGTTTTTGG

Protein sequence

>MS.gene59903.t1

VVIEFPYVFAQAIIYSSIFYSMGSFVWTVDRFIWYLFFMYLTMLYFTFYGMMTTAVTPNHHVAAIIGAPCYMLWNLFSGFMIPHKRIPIWWRWYYWANPVAWTLYGLLTSQYGDDDKLVKLTNGSSVPIRLVLKEVF