Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061947.t1 | XP_003615230.1 | 96.3 | 269 | 7 | 2 | 1 | 267 | 1 | 268 | 9.20E-74 | 287 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061947.t1 | Q9SB61 | 47.9 | 242 | 84 | 2 | 26 | 267 | 21 | 220 | 3.7e-39 | 163.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061947.t1 | G7KBF3 | 96.3 | 269 | 7 | 2 | 1 | 267 | 1 | 268 | 6.7e-74 | 287.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene061947.t1 | TF | zf-HD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene061947 | 0.818785 | 1.63E-52 | -1.69E-46 |
| MS.gene049190 | MS.gene061947 | 0.803291 | 3.74E-49 | -1.69E-46 |
| MS.gene049255 | MS.gene061947 | 0.83146 | 1.65E-55 | -1.69E-46 |
| MS.gene049260 | MS.gene061947 | 0.818106 | 2.33E-52 | -1.69E-46 |
| MS.gene049341 | MS.gene061947 | 0.852728 | 3.82E-61 | -1.69E-46 |
| MS.gene049382 | MS.gene061947 | 0.804189 | 2.43E-49 | -1.69E-46 |
| MS.gene049404 | MS.gene061947 | 0.80973 | 1.64E-50 | -1.69E-46 |
| MS.gene049821 | MS.gene061947 | 0.838532 | 2.72E-57 | -1.69E-46 |
| MS.gene049850 | MS.gene061947 | 0.822388 | 2.43E-53 | -1.69E-46 |
| MS.gene049979 | MS.gene061947 | 0.823606 | 1.27E-53 | -1.69E-46 |
| MS.gene050020 | MS.gene061947 | 0.973207 | 4.87E-136 | -1.69E-46 |
| MS.gene05016 | MS.gene061947 | 0.820838 | 5.55E-53 | -1.69E-46 |
| MS.gene050438 | MS.gene061947 | 0.805773 | 1.14E-49 | -1.69E-46 |
| MS.gene050584 | MS.gene061947 | 0.869947 | 2.11E-66 | -1.69E-46 |
| MS.gene050585 | MS.gene061947 | 0.838688 | 2.48E-57 | -1.69E-46 |
| MS.gene050586 | MS.gene061947 | 0.863293 | 2.76E-64 | -1.69E-46 |
| MS.gene050587 | MS.gene061947 | 0.827783 | 1.29E-54 | -1.69E-46 |
| MS.gene050689 | MS.gene061947 | 0.805202 | 1.50E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene061947 | 0.893954 | 3.88E-75 | -1.69E-46 |
| MS.gene050808 | MS.gene061947 | 0.868206 | 7.75E-66 | -1.69E-46 |
| MS.gene050815 | MS.gene061947 | 0.843574 | 1.29E-58 | -1.69E-46 |
| MS.gene050901 | MS.gene061947 | 0.805911 | 1.06E-49 | -1.69E-46 |
| MS.gene050959 | MS.gene061947 | 0.804162 | 2.47E-49 | -1.69E-46 |
| MS.gene051180 | MS.gene061947 | 0.806159 | 9.42E-50 | -1.69E-46 |
| MS.gene051199 | MS.gene061947 | 0.825776 | 3.90E-54 | -1.69E-46 |
| MS.gene051243 | MS.gene061947 | 0.806164 | 9.40E-50 | -1.69E-46 |
| MS.gene051328 | MS.gene061947 | 0.831527 | 1.59E-55 | -1.69E-46 |
| MS.gene05143 | MS.gene061947 | 0.800801 | 1.22E-48 | -1.69E-46 |
| MS.gene05157 | MS.gene061947 | 0.875401 | 3.17E-68 | -1.69E-46 |
| MS.gene051719 | MS.gene061947 | 0.852085 | 5.82E-61 | -1.69E-46 |
| MS.gene051720 | MS.gene061947 | 0.850651 | 1.48E-60 | -1.69E-46 |
| MS.gene051789 | MS.gene061947 | 0.873188 | 1.78E-67 | -1.69E-46 |
| MS.gene052020 | MS.gene061947 | 0.835513 | 1.61E-56 | -1.69E-46 |
| MS.gene052133 | MS.gene061947 | 0.849005 | 4.27E-60 | -1.69E-46 |
| MS.gene052142 | MS.gene061947 | 0.826317 | 2.90E-54 | -1.69E-46 |
| MS.gene052323 | MS.gene061947 | 0.810051 | 1.40E-50 | -1.69E-46 |
| MS.gene05236 | MS.gene061947 | 0.825968 | 3.51E-54 | -1.69E-46 |
| MS.gene052516 | MS.gene061947 | 0.823563 | 1.29E-53 | -1.69E-46 |
| MS.gene052611 | MS.gene061947 | 0.848051 | 7.84E-60 | -1.69E-46 |
| MS.gene052612 | MS.gene061947 | 0.827702 | 1.35E-54 | -1.69E-46 |
| MS.gene052665 | MS.gene061947 | 0.877738 | 4.94E-69 | -1.69E-46 |
| MS.gene052863 | MS.gene061947 | 0.808103 | 3.65E-50 | -1.69E-46 |
| MS.gene052879 | MS.gene061947 | 0.82558 | 4.34E-54 | -1.69E-46 |
| MS.gene052880 | MS.gene061947 | 0.813257 | 2.81E-51 | -1.69E-46 |
| MS.gene053008 | MS.gene061947 | 0.807171 | 5.76E-50 | -1.69E-46 |
| MS.gene053074 | MS.gene061947 | 0.870411 | 1.49E-66 | -1.69E-46 |
| MS.gene053174 | MS.gene061947 | 0.833609 | 4.83E-56 | -1.69E-46 |
| MS.gene053383 | MS.gene061947 | 0.841429 | 4.78E-58 | -1.69E-46 |
| MS.gene053563 | MS.gene061947 | 0.859108 | 5.20E-63 | -1.69E-46 |
| MS.gene053581 | MS.gene061947 | 0.824021 | 1.01E-53 | -1.69E-46 |
| MS.gene053630 | MS.gene061947 | 0.834222 | 3.40E-56 | -1.69E-46 |
| MS.gene053894 | MS.gene061947 | 0.83833 | 3.07E-57 | -1.69E-46 |
| MS.gene054550 | MS.gene061947 | 0.834497 | 2.90E-56 | -1.69E-46 |
| MS.gene05456 | MS.gene061947 | 0.870269 | 1.66E-66 | -1.69E-46 |
| MS.gene054596 | MS.gene061947 | 0.811072 | 8.40E-51 | -1.69E-46 |
| MS.gene05462 | MS.gene061947 | 0.802564 | 5.29E-49 | -1.69E-46 |
| MS.gene054697 | MS.gene061947 | 0.809152 | 2.18E-50 | -1.69E-46 |
| MS.gene054791 | MS.gene061947 | 0.833515 | 5.10E-56 | -1.69E-46 |
| MS.gene054833 | MS.gene061947 | 0.817395 | 3.37E-52 | -1.69E-46 |
| MS.gene055207 | MS.gene061947 | 0.808087 | 3.68E-50 | -1.69E-46 |
| MS.gene055357 | MS.gene061947 | 0.812887 | 3.38E-51 | -1.69E-46 |
| MS.gene055359 | MS.gene061947 | 0.840541 | 8.17E-58 | -1.69E-46 |
| MS.gene055465 | MS.gene061947 | 0.838017 | 3.69E-57 | -1.69E-46 |
| MS.gene055466 | MS.gene061947 | 0.851551 | 8.25E-61 | -1.69E-46 |
| MS.gene055468 | MS.gene061947 | 0.824804 | 6.62E-54 | -1.69E-46 |
| MS.gene055512 | MS.gene061947 | 0.840627 | 7.76E-58 | -1.69E-46 |
| MS.gene055608 | MS.gene061947 | 0.80306 | 4.17E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene061947 | 0.80206 | 6.71E-49 | -1.69E-46 |
| MS.gene055890 | MS.gene061947 | 0.844156 | 9.00E-59 | -1.69E-46 |
| MS.gene055891 | MS.gene061947 | 0.833875 | 4.15E-56 | -1.69E-46 |
| MS.gene055912 | MS.gene061947 | 0.844683 | 6.49E-59 | -1.69E-46 |
| MS.gene056005 | MS.gene061947 | 0.821824 | 3.29E-53 | -1.69E-46 |
| MS.gene05604 | MS.gene061947 | 0.814381 | 1.59E-51 | -1.69E-46 |
| MS.gene056069 | MS.gene061947 | -0.814759 | 1.31E-51 | -1.69E-46 |
| MS.gene056173 | MS.gene061947 | 0.81067 | 1.03E-50 | -1.69E-46 |
| MS.gene056553 | MS.gene061947 | 0.827794 | 1.28E-54 | -1.69E-46 |
| MS.gene056589 | MS.gene061947 | 0.807796 | 4.24E-50 | -1.69E-46 |
| MS.gene056643 | MS.gene061947 | 0.828798 | 7.36E-55 | -1.69E-46 |
| MS.gene056760 | MS.gene061947 | 0.806857 | 6.71E-50 | -1.69E-46 |
| MS.gene056859 | MS.gene061947 | 0.81823 | 2.19E-52 | -1.69E-46 |
| MS.gene057060 | MS.gene061947 | 0.853241 | 2.72E-61 | -1.69E-46 |
| MS.gene057444 | MS.gene061947 | 0.819999 | 8.65E-53 | -1.69E-46 |
| MS.gene057446 | MS.gene061947 | 0.800701 | 1.27E-48 | -1.69E-46 |
| MS.gene057693 | MS.gene061947 | 0.820007 | 8.61E-53 | -1.69E-46 |
| MS.gene057869 | MS.gene061947 | 0.814603 | 1.42E-51 | -1.69E-46 |
| MS.gene058168 | MS.gene061947 | 0.825994 | 3.46E-54 | -1.69E-46 |
| MS.gene059006 | MS.gene061947 | 0.891789 | 2.88E-74 | -1.69E-46 |
| MS.gene059607 | MS.gene061947 | 0.814105 | 1.82E-51 | -1.69E-46 |
| MS.gene05978 | MS.gene061947 | 0.829318 | 5.50E-55 | -1.69E-46 |
| MS.gene059937 | MS.gene061947 | 0.853983 | 1.67E-61 | -1.69E-46 |
| MS.gene060137 | MS.gene061947 | -0.826524 | 2.59E-54 | -1.69E-46 |
| MS.gene060194 | MS.gene061947 | 0.808576 | 2.89E-50 | -1.69E-46 |
| MS.gene060429 | MS.gene061947 | 0.802176 | 6.35E-49 | -1.69E-46 |
| MS.gene060470 | MS.gene061947 | 0.841987 | 3.40E-58 | -1.69E-46 |
| MS.gene060519 | MS.gene061947 | 0.802123 | 6.51E-49 | -1.69E-46 |
| MS.gene060587 | MS.gene061947 | 0.871938 | 4.66E-67 | -1.69E-46 |
| MS.gene060677 | MS.gene061947 | 0.801557 | 8.51E-49 | -1.69E-46 |
| MS.gene060691 | MS.gene061947 | 0.805487 | 1.30E-49 | -1.69E-46 |
| MS.gene060803 | MS.gene061947 | 0.826356 | 2.84E-54 | -1.69E-46 |
| MS.gene061176 | MS.gene061947 | 0.869376 | 3.24E-66 | -1.69E-46 |
| MS.gene061177 | MS.gene061947 | 0.868734 | 5.24E-66 | -1.69E-46 |
| MS.gene061178 | MS.gene061947 | 0.869318 | 3.39E-66 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061947.t1 | MTR_5g065440 | 96.654 | 269 | 6 | 2 | 1 | 267 | 1 | 268 | 0.0 | 535 |
| MS.gene061947.t1 | MTR_3g100650 | 59.794 | 291 | 76 | 7 | 1 | 267 | 1 | 274 | 1.25e-96 | 285 |
| MS.gene061947.t1 | MTR_5g032600 | 52.294 | 218 | 74 | 7 | 58 | 267 | 77 | 272 | 2.50e-56 | 182 |
| MS.gene061947.t1 | MTR_4g134580 | 41.700 | 247 | 94 | 6 | 58 | 266 | 112 | 346 | 8.22e-51 | 170 |
| MS.gene061947.t1 | MTR_1g077020 | 41.509 | 212 | 92 | 5 | 56 | 266 | 6 | 186 | 8.79e-48 | 157 |
| MS.gene061947.t1 | MTR_2g076780 | 41.038 | 212 | 109 | 4 | 59 | 266 | 37 | 236 | 8.70e-47 | 156 |
| MS.gene061947.t1 | MTR_7g010300 | 40.724 | 221 | 98 | 6 | 56 | 266 | 85 | 282 | 1.73e-44 | 153 |
| MS.gene061947.t1 | MTR_5g020510 | 39.819 | 221 | 106 | 6 | 54 | 266 | 94 | 295 | 5.11e-44 | 151 |
| MS.gene061947.t1 | MTR_8g069080 | 42.326 | 215 | 103 | 7 | 58 | 266 | 68 | 267 | 1.42e-43 | 149 |
| MS.gene061947.t1 | MTR_6g012280 | 42.857 | 210 | 102 | 5 | 59 | 266 | 91 | 284 | 1.23e-40 | 143 |
| MS.gene061947.t1 | MTR_7g070643 | 80.263 | 76 | 12 | 1 | 22 | 97 | 48 | 120 | 2.38e-35 | 125 |
| MS.gene061947.t1 | MTR_6g080300 | 86.885 | 61 | 8 | 0 | 37 | 97 | 32 | 92 | 2.13e-31 | 112 |
| MS.gene061947.t1 | MTR_8g069380 | 47.872 | 94 | 49 | 0 | 174 | 267 | 22 | 115 | 3.42e-22 | 89.0 |
| MS.gene061947.t1 | MTR_8g051480 | 54.667 | 75 | 33 | 1 | 192 | 266 | 55 | 128 | 1.82e-20 | 84.7 |
| MS.gene061947.t1 | MTR_3g039810 | 57.534 | 73 | 24 | 2 | 57 | 129 | 18 | 83 | 2.03e-20 | 83.6 |
| MS.gene061947.t1 | MTR_6g073013 | 53.333 | 60 | 27 | 1 | 204 | 262 | 101 | 160 | 1.92e-16 | 75.5 |
| MS.gene061947.t1 | MTR_6g073013 | 56.000 | 50 | 21 | 1 | 59 | 107 | 19 | 68 | 1.80e-11 | 62.0 |
| MS.gene061947.t1 | MTR_6g012220 | 56.604 | 53 | 19 | 1 | 55 | 107 | 17 | 65 | 6.40e-13 | 63.2 |
| MS.gene061947.t1 | MTR_2g007810 | 100.000 | 29 | 0 | 0 | 69 | 97 | 19 | 47 | 7.55e-13 | 63.5 |
| MS.gene061947.t1 | MTR_8g467340 | 54.545 | 55 | 23 | 1 | 56 | 108 | 61 | 115 | 1.43e-12 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061947.t1 | AT4G24660 | 48.289 | 263 | 88 | 7 | 7 | 267 | 4 | 220 | 2.30e-65 | 203 |
| MS.gene061947.t1 | AT4G24660 | 47.893 | 261 | 84 | 8 | 7 | 263 | 4 | 216 | 3.17e-62 | 196 |
| MS.gene061947.t1 | AT2G02540 | 38.868 | 265 | 123 | 7 | 18 | 266 | 43 | 284 | 3.63e-58 | 188 |
| MS.gene061947.t1 | AT2G02540 | 38.868 | 265 | 123 | 7 | 18 | 266 | 43 | 284 | 3.63e-58 | 188 |
| MS.gene061947.t1 | AT1G14440 | 40.566 | 212 | 105 | 3 | 57 | 266 | 88 | 280 | 6.42e-52 | 172 |
| MS.gene061947.t1 | AT1G14440 | 40.566 | 212 | 105 | 3 | 57 | 266 | 88 | 280 | 6.42e-52 | 172 |
| MS.gene061947.t1 | AT5G65410 | 43.439 | 221 | 90 | 3 | 50 | 267 | 66 | 254 | 3.52e-50 | 166 |
| MS.gene061947.t1 | AT2G18350 | 41.981 | 212 | 90 | 4 | 57 | 266 | 80 | 260 | 2.15e-47 | 159 |
| MS.gene061947.t1 | AT1G75240 | 40.693 | 231 | 118 | 2 | 56 | 267 | 73 | 303 | 7.50e-47 | 159 |
| MS.gene061947.t1 | AT3G50890 | 40.566 | 212 | 94 | 3 | 58 | 265 | 59 | 242 | 1.55e-43 | 148 |
| MS.gene061947.t1 | AT1G69600 | 40.376 | 213 | 95 | 5 | 57 | 263 | 29 | 215 | 5.82e-38 | 134 |
| MS.gene061947.t1 | AT5G15210 | 36.530 | 219 | 93 | 5 | 54 | 260 | 51 | 235 | 9.18e-37 | 131 |
| MS.gene061947.t1 | AT5G39760 | 36.667 | 210 | 128 | 2 | 59 | 266 | 56 | 262 | 9.92e-36 | 130 |
| MS.gene061947.t1 | AT3G28920 | 36.916 | 214 | 116 | 4 | 57 | 263 | 50 | 251 | 3.01e-35 | 128 |
| MS.gene061947.t1 | AT5G60480 | 38.164 | 207 | 89 | 7 | 56 | 260 | 34 | 203 | 1.05e-30 | 114 |
| MS.gene061947.t1 | AT1G74660 | 67.647 | 68 | 20 | 1 | 55 | 122 | 35 | 100 | 1.50e-24 | 94.7 |
| MS.gene061947.t1 | AT1G74660 | 70.492 | 61 | 18 | 0 | 55 | 115 | 35 | 95 | 1.59e-24 | 94.7 |
| MS.gene061947.t1 | AT3G28917 | 65.217 | 69 | 21 | 2 | 56 | 123 | 30 | 96 | 1.27e-23 | 92.4 |
| MS.gene061947.t1 | AT1G18835 | 63.793 | 58 | 18 | 1 | 57 | 114 | 24 | 78 | 5.20e-19 | 79.7 |
| MS.gene061947.t1 | AT5G42780 | 36.232 | 69 | 44 | 0 | 195 | 263 | 170 | 238 | 7.14e-11 | 61.2 |
| MS.gene061947.t1 | AT5G42780 | 57.692 | 52 | 18 | 2 | 59 | 109 | 64 | 112 | 7.28e-11 | 61.2 |
Find 82 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCACTGGTGTTTGTGTTTC+TGG | 0.083748 | 5.3:-51674317 | None:intergenic |
| AGAGAAATGTTCTTAAAGTT+TGG | 0.256503 | 5.3:+51675014 | MS.gene061947:CDS |
| GCTCTCCCTGCAGCTGCTTC+CGG | 0.322915 | 5.3:+51674770 | MS.gene061947:CDS |
| GGTGGTGGTGGTAGCGGTGG+TGG | 0.325308 | 5.3:+51674851 | MS.gene061947:CDS |
| CCTGCAGCTGCTTCCGGTGG+TGG | 0.348678 | 5.3:+51674776 | MS.gene061947:CDS |
| GGAGGTGGTGGTGGTAGCGG+TGG | 0.371127 | 5.3:+51674848 | MS.gene061947:CDS |
| GGAGGAGGTGGTGGTGGTAG+CGG | 0.383614 | 5.3:+51674845 | MS.gene061947:CDS |
| TCTCCTTGCGGTGGAAGTTC+CGG | 0.388446 | 5.3:-51674574 | None:intergenic |
| GTGGAGGTGGTGGCTGTGAC+CGG | 0.391995 | 5.3:-51674625 | None:intergenic |
| TGGTATTGCGGCGGTGGAGG+TGG | 0.407593 | 5.3:-51674638 | None:intergenic |
| CCACCACCGGAAGCAGCTGC+AGG | 0.423625 | 5.3:-51674776 | None:intergenic |
| TGATGGTATTGCGGCGGTGG+AGG | 0.428238 | 5.3:-51674641 | None:intergenic |
| AAGAATCACGCCGTTAGCTT+CGG | 0.428386 | 5.3:+51674461 | MS.gene061947:CDS |
| TTTATCGCTGCCGGTGAAGA+AGG | 0.429195 | 5.3:+51674512 | MS.gene061947:CDS |
| TGGTGATGGTATTGCGGCGG+TGG | 0.436665 | 5.3:-51674644 | None:intergenic |
| GGTGGTGGTAGCGGTGGTGG+TGG | 0.439608 | 5.3:+51674854 | MS.gene061947:CDS |
| AGTAGCGGTGGAGGAGGTGG+TGG | 0.442867 | 5.3:+51674836 | MS.gene061947:CDS |
| TATTGCGGCGGTGGAGGTGG+TGG | 0.444382 | 5.3:-51674635 | None:intergenic |
| AGCAGTAGCGGTGGAGGAGG+TGG | 0.444551 | 5.3:+51674833 | MS.gene061947:CDS |
| TTCGGTGGCCATGCTGTTGA+CGG | 0.447816 | 5.3:+51674479 | MS.gene061947:CDS |
| CACAATAACAAGAACACTCT+TGG | 0.453033 | 5.3:+51675040 | MS.gene061947:CDS |
| GGAGGAGCACGGTGGTAGTA+AGG | 0.453304 | 5.3:-51674683 | None:intergenic |
| CGTGATTCTTCTGACATTCT+CGG | 0.457636 | 5.3:-51674448 | None:intergenic |
| GGAACAGAAAGAGAAGTTGC+TGG | 0.466315 | 5.3:+51674910 | MS.gene061947:CDS |
| CCTCCTCCACCGCTACTGCT+AGG | 0.470793 | 5.3:-51674830 | None:intergenic |
| AAACTCACCGTTGGAGTTGA+CGG | 0.483943 | 5.3:+51674380 | MS.gene061947:CDS |
| AGCGGTGGAGGAGGTGGTGG+TGG | 0.484419 | 5.3:+51674839 | MS.gene061947:CDS |
| GTAGCGGTGGTGGTGGATCA+AGG | 0.485672 | 5.3:+51674861 | MS.gene061947:CDS |
| AAACGGTTAGTTCATGTAAC+CGG | 0.491216 | 5.3:+51674606 | MS.gene061947:CDS |
| AACGGTCTGTGATGTGCCAC+CGG | 0.497928 | 5.3:-51674746 | None:intergenic |
| ATGATGATGGTGAAGGTAAC+CGG | 0.500422 | 5.3:-51674714 | None:intergenic |
| CAAGGAGATCGATGGTGAAA+CGG | 0.502038 | 5.3:+51674589 | MS.gene061947:CDS |
| AGAGGAACAAGAAGAGGAGA+TGG | 0.506205 | 5.3:+51674289 | MS.gene061947:CDS |
| TTCAAGTGTTCCTTCTTCAC+CGG | 0.506613 | 5.3:-51674522 | None:intergenic |
| CGTGCTCCTCCTTCCACAGC+CGG | 0.511664 | 5.3:+51674695 | MS.gene061947:CDS |
| TTCTCTGCTCATTCCACCAC+CGG | 0.513977 | 5.3:-51674789 | None:intergenic |
| AACCCTAGCAGTAGCGGTGG+AGG | 0.514729 | 5.3:+51674827 | MS.gene061947:CDS |
| CTCCTCCACCGCTACTGCTA+GGG | 0.520409 | 5.3:-51674829 | None:intergenic |
| ATGGTGAAGGTAACCGGCTG+TGG | 0.521317 | 5.3:-51674708 | None:intergenic |
| GCGCCGCTTGTAACTGTCAC+CGG | 0.526555 | 5.3:+51674555 | MS.gene061947:CDS |
| CCATCATCATCTTGCAACTC+CGG | 0.527038 | 5.3:+51674727 | MS.gene061947:CDS |
| CTCCCTGCAGCTGCTTCCGG+TGG | 0.528838 | 5.3:+51674773 | MS.gene061947:CDS |
| AATCACGCCGTTAGCTTCGG+TGG | 0.536570 | 5.3:+51674464 | MS.gene061947:CDS |
| GAGTCATAACTCGTTGGCAC+TGG | 0.537593 | 5.3:-51674332 | None:intergenic |
| CCTAGCAGTAGCGGTGGAGG+AGG | 0.539653 | 5.3:+51674830 | MS.gene061947:CDS |
| CACCATCGATCTCCTTGCGG+TGG | 0.556188 | 5.3:-51674583 | None:intergenic |
| GCAGCTGCAGGGAGAGCCAA+CGG | 0.558422 | 5.3:-51674764 | None:intergenic |
| TGTTGCGAGTTTATCGCTGC+CGG | 0.567002 | 5.3:+51674503 | MS.gene061947:CDS |
| TGAAGGTAACCGGCTGTGGA+AGG | 0.577589 | 5.3:-51674704 | None:intergenic |
| CACCACCGGAAGCAGCTGCA+GGG | 0.578947 | 5.3:-51674775 | None:intergenic |
| TCACGTTCAAAACTCACCGT+TGG | 0.581295 | 5.3:+51674371 | MS.gene061947:CDS |
| AGAATTGATTGCTGTGGTGA+TGG | 0.582941 | 5.3:-51674658 | None:intergenic |
| AATGGAATTTGATGAACAAG+AGG | 0.584250 | 5.3:+51674271 | None:intergenic |
| TTTCCTTCCGTCAACTCCAA+CGG | 0.588914 | 5.3:-51674387 | None:intergenic |
| TGAACAAGAGGAACAAGAAG+AGG | 0.593455 | 5.3:+51674283 | MS.gene061947:CDS |
| CCGGAGTTGCAAGATGATGA+TGG | 0.603279 | 5.3:-51674727 | None:intergenic |
| GTTGGAGTTGACGGAAGGAA+AGG | 0.610107 | 5.3:+51674389 | MS.gene061947:CDS |
| CCAACGAGTTATGACTCACT+TGG | 0.611073 | 5.3:+51674338 | MS.gene061947:CDS |
| CGCAACAACCGTCAACAGCA+TGG | 0.612162 | 5.3:-51674487 | None:intergenic |
| AGGTAACCGGCTGTGGAAGG+AGG | 0.612370 | 5.3:-51674701 | None:intergenic |
| GGTGGCACATCACAGACCGT+TGG | 0.612785 | 5.3:+51674748 | MS.gene061947:CDS |
| ATTCAGAACAAAATTCACTC+AGG | 0.624697 | 5.3:+51674889 | MS.gene061947:CDS |
| TTGCTGTGGTGATGGTATTG+CGG | 0.630881 | 5.3:-51674650 | None:intergenic |
| TCACCGGAACTTCCACCGCA+AGG | 0.633282 | 5.3:+51674571 | MS.gene061947:CDS |
| ATATCAAACCCTAGCAGTAG+CGG | 0.637238 | 5.3:+51674821 | MS.gene061947:CDS |
| TCAAACCCTAGCAGTAGCGG+TGG | 0.637366 | 5.3:+51674824 | MS.gene061947:CDS |
| GGAAGGAAAGGTAACGGCAA+CGG | 0.639375 | 5.3:+51674401 | MS.gene061947:CDS |
| TCACCGTTGGAGTTGACGGA+AGG | 0.640977 | 5.3:+51674384 | MS.gene061947:CDS |
| TTCCACCGCAAGGAGATCGA+TGG | 0.642395 | 5.3:+51674581 | MS.gene061947:CDS |
| CCAAGTGAGTCATAACTCGT+TGG | 0.644449 | 5.3:-51674338 | None:intergenic |
| TCATCATCTTGCAACTCCGG+TGG | 0.648426 | 5.3:+51674730 | MS.gene061947:CDS |
| GTTGACGGAAGGAAAGGTAA+CGG | 0.669388 | 5.3:+51674395 | MS.gene061947:CDS |
| TTTCACCATCGATCTCCTTG+CGG | 0.669478 | 5.3:-51674586 | None:intergenic |
| CGGCTGTGGAAGGAGGAGCA+CGG | 0.673027 | 5.3:-51674694 | None:intergenic |
| TTGCAAGATGATGATGGTGA+AGG | 0.675327 | 5.3:-51674721 | None:intergenic |
| AGCATGGCCACCGAAGCTAA+CGG | 0.680090 | 5.3:-51674471 | None:intergenic |
| CTGTGGTGATGGTATTGCGG+CGG | 0.691893 | 5.3:-51674647 | None:intergenic |
| AGGAAAGGTAACGGCAACGG+CGG | 0.697533 | 5.3:+51674404 | MS.gene061947:CDS |
| AAGGCGAGAATTGATTGCTG+TGG | 0.722344 | 5.3:-51674664 | None:intergenic |
| GTTCCGGTGACAGTTACAAG+CGG | 0.723013 | 5.3:-51674558 | None:intergenic |
| ACATTCTCGGTACCTCACCG+TGG | 0.724754 | 5.3:-51674435 | None:intergenic |
| CTGTGGAAGGAGGAGCACGG+TGG | 0.754237 | 5.3:-51674691 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAGAAATGTTCTTAAAGTT+TGG | + | chr5.3:51675014-51675033 | MS.gene061947:CDS | 25.0% |
| ATTCAGAACAAAATTCACTC+AGG | + | chr5.3:51674889-51674908 | MS.gene061947:CDS | 30.0% | |
| CACAATAACAAGAACACTCT+TGG | + | chr5.3:51675040-51675059 | MS.gene061947:CDS | 35.0% | |
| ! | AAACGGTTAGTTCATGTAAC+CGG | + | chr5.3:51674606-51674625 | MS.gene061947:CDS | 35.0% |
| AGAATTGATTGCTGTGGTGA+TGG | - | chr5.3:51674661-51674680 | None:intergenic | 40.0% | |
| ATATCAAACCCTAGCAGTAG+CGG | + | chr5.3:51674821-51674840 | MS.gene061947:CDS | 40.0% | |
| CGTGATTCTTCTGACATTCT+CGG | - | chr5.3:51674451-51674470 | None:intergenic | 40.0% | |
| TGAACAAGAGGAACAAGAAG+AGG | + | chr5.3:51674283-51674302 | MS.gene061947:CDS | 40.0% | |
| TTCAAGTGTTCCTTCTTCAC+CGG | - | chr5.3:51674525-51674544 | None:intergenic | 40.0% | |
| ! | CTTTTGCTGAAGAACATGGT+TGG | + | chr5.3:51674933-51674952 | MS.gene061947:CDS | 40.0% |
| !! | ATGATGATGGTGAAGGTAAC+CGG | - | chr5.3:51674717-51674736 | None:intergenic | 40.0% |
| !! | TTGCAAGATGATGATGGTGA+AGG | - | chr5.3:51674724-51674743 | None:intergenic | 40.0% |
| AAACTCACCGTTGGAGTTGA+CGG | + | chr5.3:51674380-51674399 | MS.gene061947:CDS | 45.0% | |
| AAGGCGAGAATTGATTGCTG+TGG | - | chr5.3:51674667-51674686 | None:intergenic | 45.0% | |
| AGAGGAACAAGAAGAGGAGA+TGG | + | chr5.3:51674289-51674308 | MS.gene061947:CDS | 45.0% | |
| CAAGGAGATCGATGGTGAAA+CGG | + | chr5.3:51674589-51674608 | MS.gene061947:CDS | 45.0% | |
| CCAACGAGTTATGACTCACT+TGG | + | chr5.3:51674338-51674357 | MS.gene061947:CDS | 45.0% | |
| CCAAGTGAGTCATAACTCGT+TGG | - | chr5.3:51674341-51674360 | None:intergenic | 45.0% | |
| CCATCATCATCTTGCAACTC+CGG | + | chr5.3:51674727-51674746 | MS.gene061947:CDS | 45.0% | |
| GGAACAGAAAGAGAAGTTGC+TGG | + | chr5.3:51674910-51674929 | MS.gene061947:CDS | 45.0% | |
| GTTGACGGAAGGAAAGGTAA+CGG | + | chr5.3:51674395-51674414 | MS.gene061947:CDS | 45.0% | |
| TCACGTTCAAAACTCACCGT+TGG | + | chr5.3:51674371-51674390 | MS.gene061947:CDS | 45.0% | |
| TTTCACCATCGATCTCCTTG+CGG | - | chr5.3:51674589-51674608 | None:intergenic | 45.0% | |
| TTTCCTTCCGTCAACTCCAA+CGG | - | chr5.3:51674390-51674409 | None:intergenic | 45.0% | |
| ! | AAGAATCACGCCGTTAGCTT+CGG | + | chr5.3:51674461-51674480 | MS.gene061947:CDS | 45.0% |
| !! | CTGGCTTTTGCTGAAGAACA+TGG | + | chr5.3:51674929-51674948 | MS.gene061947:CDS | 45.0% |
| !! | TTGCTGTGGTGATGGTATTG+CGG | - | chr5.3:51674653-51674672 | None:intergenic | 45.0% |
| CCGGAGTTGCAAGATGATGA+TGG | - | chr5.3:51674730-51674749 | None:intergenic | 50.0% | |
| GAGTCATAACTCGTTGGCAC+TGG | - | chr5.3:51674335-51674354 | None:intergenic | 50.0% | |
| GTTCCGGTGACAGTTACAAG+CGG | - | chr5.3:51674561-51674580 | None:intergenic | 50.0% | |
| GTTGGAGTTGACGGAAGGAA+AGG | + | chr5.3:51674389-51674408 | MS.gene061947:CDS | 50.0% | |
| TCATCATCTTGCAACTCCGG+TGG | + | chr5.3:51674730-51674749 | MS.gene061947:CDS | 50.0% | |
| TGTTGCGAGTTTATCGCTGC+CGG | + | chr5.3:51674503-51674522 | MS.gene061947:CDS | 50.0% | |
| TTCTCTGCTCATTCCACCAC+CGG | - | chr5.3:51674792-51674811 | None:intergenic | 50.0% | |
| TTTATCGCTGCCGGTGAAGA+AGG | + | chr5.3:51674512-51674531 | MS.gene061947:CDS | 50.0% | |
| ! | GGAAGGAAAGGTAACGGCAA+CGG | + | chr5.3:51674401-51674420 | MS.gene061947:CDS | 50.0% |
| !! | GGCACTGGTGTTTGTGTTTC+TGG | - | chr5.3:51674320-51674339 | None:intergenic | 50.0% |
| AACGGTCTGTGATGTGCCAC+CGG | - | chr5.3:51674749-51674768 | None:intergenic | 55.0% | |
| ACATTCTCGGTACCTCACCG+TGG | - | chr5.3:51674438-51674457 | None:intergenic | 55.0% | |
| AGCATGGCCACCGAAGCTAA+CGG | - | chr5.3:51674474-51674493 | None:intergenic | 55.0% | |
| ATGGTGAAGGTAACCGGCTG+TGG | - | chr5.3:51674711-51674730 | None:intergenic | 55.0% | |
| CGCAACAACCGTCAACAGCA+TGG | - | chr5.3:51674490-51674509 | None:intergenic | 55.0% | |
| TCAAACCCTAGCAGTAGCGG+TGG | + | chr5.3:51674824-51674843 | MS.gene061947:CDS | 55.0% | |
| TCACCGTTGGAGTTGACGGA+AGG | + | chr5.3:51674384-51674403 | MS.gene061947:CDS | 55.0% | |
| TCTCCTTGCGGTGGAAGTTC+CGG | - | chr5.3:51674577-51674596 | None:intergenic | 55.0% | |
| TGAAGGTAACCGGCTGTGGA+AGG | - | chr5.3:51674707-51674726 | None:intergenic | 55.0% | |
| TTCCACCGCAAGGAGATCGA+TGG | + | chr5.3:51674581-51674600 | MS.gene061947:CDS | 55.0% | |
| TTCGGTGGCCATGCTGTTGA+CGG | + | chr5.3:51674479-51674498 | MS.gene061947:CDS | 55.0% | |
| ! | AATCACGCCGTTAGCTTCGG+TGG | + | chr5.3:51674464-51674483 | MS.gene061947:CDS | 55.0% |
| ! | AGGAAAGGTAACGGCAACGG+CGG | + | chr5.3:51674404-51674423 | MS.gene061947:CDS | 55.0% |
| ! | CAACGGCGGTTTTGCTTCCA+CGG | + | chr5.3:51674418-51674437 | MS.gene061947:CDS | 55.0% |
| !! | CTGTGGTGATGGTATTGCGG+CGG | - | chr5.3:51674650-51674669 | None:intergenic | 55.0% |
| AACCCTAGCAGTAGCGGTGG+AGG | + | chr5.3:51674827-51674846 | MS.gene061947:CDS | 60.0% | |
| AGGTAACCGGCTGTGGAAGG+AGG | - | chr5.3:51674704-51674723 | None:intergenic | 60.0% | |
| CACCATCGATCTCCTTGCGG+TGG | - | chr5.3:51674586-51674605 | None:intergenic | 60.0% | |
| CTCCTCCACCGCTACTGCTA+GGG | - | chr5.3:51674832-51674851 | None:intergenic | 60.0% | |
| GCGCCGCTTGTAACTGTCAC+CGG | + | chr5.3:51674555-51674574 | MS.gene061947:CDS | 60.0% | |
| GGTGGCACATCACAGACCGT+TGG | + | chr5.3:51674748-51674767 | MS.gene061947:CDS | 60.0% | |
| TCACCGGAACTTCCACCGCA+AGG | + | chr5.3:51674571-51674590 | MS.gene061947:CDS | 60.0% | |
| TGATGGTATTGCGGCGGTGG+AGG | - | chr5.3:51674644-51674663 | None:intergenic | 60.0% | |
| ! | GCGGTTTTGCTTCCACGGTG+AGG | + | chr5.3:51674423-51674442 | MS.gene061947:CDS | 60.0% |
| ! | GGAGGAGCACGGTGGTAGTA+AGG | - | chr5.3:51674686-51674705 | None:intergenic | 60.0% |
| !! | GTAGCGGTGGTGGTGGATCA+AGG | + | chr5.3:51674861-51674880 | MS.gene061947:CDS | 60.0% |
| !! | TGGTGATGGTATTGCGGCGG+TGG | - | chr5.3:51674647-51674666 | None:intergenic | 60.0% |
| AGCAGTAGCGGTGGAGGAGG+TGG | + | chr5.3:51674833-51674852 | MS.gene061947:CDS | 65.0% | |
| AGTAGCGGTGGAGGAGGTGG+TGG | + | chr5.3:51674836-51674855 | MS.gene061947:CDS | 65.0% | |
| CACCACCGGAAGCAGCTGCA+GGG | - | chr5.3:51674778-51674797 | None:intergenic | 65.0% | |
| CCTAGCAGTAGCGGTGGAGG+AGG | + | chr5.3:51674830-51674849 | MS.gene061947:CDS | 65.0% | |
| CCTCCTCCACCGCTACTGCT+AGG | - | chr5.3:51674833-51674852 | None:intergenic | 65.0% | |
| CGGCTGTGGAAGGAGGAGCA+CGG | - | chr5.3:51674697-51674716 | None:intergenic | 65.0% | |
| CGTGCTCCTCCTTCCACAGC+CGG | + | chr5.3:51674695-51674714 | MS.gene061947:CDS | 65.0% | |
| GCAGCTGCAGGGAGAGCCAA+CGG | - | chr5.3:51674767-51674786 | None:intergenic | 65.0% | |
| GCTCTCCCTGCAGCTGCTTC+CGG | + | chr5.3:51674770-51674789 | MS.gene061947:CDS | 65.0% | |
| TATTGCGGCGGTGGAGGTGG+TGG | - | chr5.3:51674638-51674657 | None:intergenic | 65.0% | |
| TGGTATTGCGGCGGTGGAGG+TGG | - | chr5.3:51674641-51674660 | None:intergenic | 65.0% | |
| ! | CTGTGGAAGGAGGAGCACGG+TGG | - | chr5.3:51674694-51674713 | None:intergenic | 65.0% |
| ! | GTGGAGGTGGTGGCTGTGAC+CGG | - | chr5.3:51674628-51674647 | None:intergenic | 65.0% |
| !! | GGAGGAGGTGGTGGTGGTAG+CGG | + | chr5.3:51674845-51674864 | MS.gene061947:CDS | 65.0% |
| CCACCACCGGAAGCAGCTGC+AGG | - | chr5.3:51674779-51674798 | None:intergenic | 70.0% | |
| CCTGCAGCTGCTTCCGGTGG+TGG | + | chr5.3:51674776-51674795 | MS.gene061947:CDS | 70.0% | |
| CTCCCTGCAGCTGCTTCCGG+TGG | + | chr5.3:51674773-51674792 | MS.gene061947:CDS | 70.0% | |
| ! | AGCGGTGGAGGAGGTGGTGG+TGG | + | chr5.3:51674839-51674858 | MS.gene061947:CDS | 70.0% |
| !! | GGAGGTGGTGGTGGTAGCGG+TGG | + | chr5.3:51674848-51674867 | MS.gene061947:CDS | 70.0% |
| !! | GGTGGTGGTAGCGGTGGTGG+TGG | + | chr5.3:51674854-51674873 | MS.gene061947:CDS | 70.0% |
| !! | GGTGGTGGTGGTAGCGGTGG+TGG | + | chr5.3:51674851-51674870 | MS.gene061947:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 51674272 | 51675075 | 51674272 | ID=MS.gene061947 |
| chr5.3 | mRNA | 51674272 | 51675075 | 51674272 | ID=MS.gene061947.t1;Parent=MS.gene061947 |
| chr5.3 | exon | 51674272 | 51675075 | 51674272 | ID=MS.gene061947.t1.exon1;Parent=MS.gene061947.t1 |
| chr5.3 | CDS | 51674272 | 51675075 | 51674272 | ID=cds.MS.gene061947.t1;Parent=MS.gene061947.t1 |
| Gene Sequence |
| Protein sequence |
