AlfalfaGEDB | MS.gene33527

M. sativa cultivar XinJiangDaYe / MS.gene33527

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016787 GO:0019239
PFAM: PF00962.25

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33527.t1	XP_003610403.1	96.2	365	14	0	1	365	12	376	1.50E-196	695.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33527.t1	Q6DHV7	37.6	359	180	5	7	355	18	342	2.8e-61	237.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33527.t1	G7JI13	96.2	365	14	0	1	365	12	376	1.1e-196	695.3

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049378	MS.gene33527	0.802563	5.29E-49	-1.69E-46
MS.gene049501	MS.gene33527	0.817626	2.99E-52	-1.69E-46
MS.gene050020	MS.gene33527	0.83222	1.07E-55	-1.69E-46
MS.gene050137	MS.gene33527	0.809473	1.86E-50	-1.69E-46
MS.gene050201	MS.gene33527	0.817732	2.83E-52	-1.69E-46
MS.gene050226	MS.gene33527	0.81121	7.84E-51	-1.69E-46
MS.gene050227	MS.gene33527	0.835802	1.36E-56	-1.69E-46
MS.gene050228	MS.gene33527	0.820746	5.83E-53	-1.69E-46
MS.gene050330	MS.gene33527	-0.81828	2.13E-52	-1.69E-46
MS.gene050438	MS.gene33527	0.81358	2.38E-51	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33527.t1	MTR_4g131840	96.164	365	14	0	1	365	12	376	0.0	721
MS.gene33527.t1	MTR_4g131840	95.870	339	14	0	27	365	41	379	0.0	663
MS.gene33527.t1	MTR_2g022780	69.482	367	101	2	3	367	5	362	0.0	522
MS.gene33527.t1	MTR_2g022780	67.630	346	101	2	24	367	13	349	3.46e-170	478
MS.gene33527.t1	MTR_2g022780	70.642	327	85	2	3	327	5	322	2.47e-168	473

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33527.t1	AT4G04880	66.575	362	110	1	1	362	1	351	2.10e-176	494
MS.gene33527.t1	AT4G04880	61.637	391	110	2	1	362	1	380	2.02e-170	480
MS.gene33527.t1	AT4G04880	68.889	270	73	1	1	270	1	259	2.34e-131	377

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Find 77 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGCTGCTTCTTCATT+TGG	0.270139	8.2:-3471070	MS.gene33527:intron
AAATGATGAGCATCAATTGA+TGG	0.417937	8.2:+3471549	None:intergenic
AATGATATACCATTCACCAA+CGG	0.687223	8.2:+3472287	None:intergenic
ACATGCATGTTTCTTTGAGG+AGG	0.579495	8.2:-3471973	MS.gene33527:CDS
ACTAAAATTTGCTCGAGAAC+AGG	0.322893	8.2:-3472163	MS.gene33527:CDS
AGACGATCGTTCATTATCTG+AGG	0.628996	8.2:-3473159	MS.gene33527:CDS
AGAGCGTTTGCTCATTCCTT+TGG	0.345646	8.2:+3472667	None:intergenic
AGCAAATGATCTGGAGCTCT+AGG	0.556308	8.2:-3470855	None:intergenic
AGCGAACTTGCTAAAGGTTT+GGG	0.357859	8.2:-3473693	MS.gene33527:CDS
AGGTCTAAGATCTGTCAGTT+CGG	0.564884	8.2:-3472624	MS.gene33527:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACGGTAAAAGAGTGATAA+TGG	+	chr8.2:3471101-3471120	None:intergenic	30.0%
AAATCATTTGGGCAAACTTA+AGG	-	chr8.2:3472287-3472306	MS.gene33527:intron	30.0%
AAATGATGAGCATCAATTGA+TGG	+	chr8.2:3473157-3473176	None:intergenic	30.0%
AATGATATACCATTCACCAA+CGG	+	chr8.2:3472419-3472438	None:intergenic	30.0%
ACACGATTACTCCAAATTTA+GGG	-	chr8.2:3471136-3471155	MS.gene33527:CDS	30.0%
AGGTGCTAGAATATCATATT+AGG	-	chr8.2:3471255-3471274	MS.gene33527:intron	30.0%
ATGAAGTTGAGAATACAATG+AGG	+	chr8.2:3473242-3473261	None:intergenic	30.0%
ATGCAGAAAAATGAATCCAA+AGG	-	chr8.2:3472020-3472039	MS.gene33527:CDS	30.0%
CAAAGAAGCAGATTATTCAA+AGG	+	chr8.2:3472326-3472345	None:intergenic	30.0%
GTAAGAATAGTAGTCTAGAT+GGG	-	chr8.2:3471317-3471336	MS.gene33527:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	3470856	3473869	3470856	ID=MS.gene33527
chr8.2	mRNA	3470856	3473869	3470856	ID=MS.gene33527.t1;Parent=MS.gene33527
chr8.2	exon	3473793	3473869	3473793	ID=MS.gene33527.t1.exon1;Parent=MS.gene33527.t1
chr8.2	CDS	3473793	3473869	3473793	ID=cds.MS.gene33527.t1;Parent=MS.gene33527.t1
chr8.2	exon	3473643	3473713	3473643	ID=MS.gene33527.t1.exon2;Parent=MS.gene33527.t1
chr8.2	CDS	3473643	3473713	3473643	ID=cds.MS.gene33527.t1;Parent=MS.gene33527.t1
chr8.2	exon	3473088	3473179	3473088	ID=MS.gene33527.t1.exon3;Parent=MS.gene33527.t1
chr8.2	CDS	3473088	3473179	3473088	ID=cds.MS.gene33527.t1;Parent=MS.gene33527.t1
chr8.2	exon	3472784	3472843	3472784	ID=MS.gene33527.t1.exon4;Parent=MS.gene33527.t1
chr8.2	CDS	3472784	3472843	3472784	ID=cds.MS.gene33527.t1;Parent=MS.gene33527.t1
chr8.2	exon	3472454	3472699	3472454	ID=MS.gene33527.t1.exon5;Parent=MS.gene33527.t1
chr8.2	CDS	3472454	3472699	3472454	ID=cds.MS.gene33527.t1;Parent=MS.gene33527.t1
chr8.2	exon	3472297	3472373	3472297	ID=MS.gene33527.t1.exon6;Parent=MS.gene33527.t1
chr8.2	CDS	3472297	3472373	3472297	ID=cds.MS.gene33527.t1;Parent=MS.gene33527.t1
chr8.2	exon	3472134	3472203	3472134	ID=MS.gene33527.t1.exon7;Parent=MS.gene33527.t1
chr8.2	CDS	3472134	3472203	3472134	ID=cds.MS.gene33527.t1;Parent=MS.gene33527.t1
chr8.2	exon	3471938	3472051	3471938	ID=MS.gene33527.t1.exon8;Parent=MS.gene33527.t1
chr8.2	CDS	3471938	3472051	3471938	ID=cds.MS.gene33527.t1;Parent=MS.gene33527.t1
chr8.2	exon	3471547	3471613	3471547	ID=MS.gene33527.t1.exon9;Parent=MS.gene33527.t1
chr8.2	CDS	3471547	3471613	3471547	ID=cds.MS.gene33527.t1;Parent=MS.gene33527.t1
chr8.2	exon	3471071	3471175	3471071	ID=MS.gene33527.t1.exon10;Parent=MS.gene33527.t1
chr8.2	CDS	3471071	3471175	3471071	ID=cds.MS.gene33527.t1;Parent=MS.gene33527.t1
chr8.2	exon	3470856	3470980	3470856	ID=MS.gene33527.t1.exon11;Parent=MS.gene33527.t1
chr8.2	CDS	3470856	3470980	3470856	ID=cds.MS.gene33527.t1;Parent=MS.gene33527.t1

Gene Sequence

>MS.gene33527

ATGGAGTGGTGTATGTCAATTCCAAAGGTAGAATTGCATGCTCACCTCAATGGATCCATCAGAGACTCCACACTTCTGTATGTTTCAAATCTTTTGCTTTTTCGACCCCGTTTTCCAACACTCTCAATTTTACTTTAATCTTTTTCTTTTTCCCAGCGAACTTGCTAAAGGTTTGGGTGATAAGGGTCTCATTGATTTCTCCCAAGTCCAGCATGTTATCCTCAAAAGTATGTATTTCCCTTCCATTATCACTCTTTTACCGTTTCTTTTACAGTATATCACACGATTACTCCAAATTTAGGGACCAACATTTCTGTTTACTCCAAATTAAATGGCCTGTATTTATTACCAGCATTTATTAGAGAGAAATTAGATAGCCCATGTAGTGGAGACTGGAGAAGGTGCTAGAATATCATATTAGGTGGTTTAAACATGTTTGGAAGAGATTTGTTGAAGCAGCGGTAAGAATAGTAGTCTAGATGGGGACAATACAATAACTAGAGATAGAGGGAGACCAAAGAAAATTATATTTAAACCATTGAGAAGGATTTAGAAGCAAATGGTCAATTAGTGTGCTTAATACATGACAGAATGTTAGGGTGTTGACTCATCCAAGCAGAAGATCGCACCTAATGTGCTAAAAAGACTCAACCGCTTTTGCTAACGTTGGTTATGGTTTTGTCAATACAGACGATCGTTCATTATCTGAGGTATTCAAGCTGTTTGACGTAATCCACATTCTTACAACTGATCATGCGACGGTTACAAGAATTACGAAAGAAGTATGTCTGACAGCACTTGGGTGACATAATGTCACCGTGACATTAAATCTGGGCCACACAACACACATTGCCATTTGCATATGGGCCACACAACACATCCATGAGTACATTTGTGTGGCCCAGATTTAATGTCACGGTGACATTATGTCACCCAAGTATTTTCCGCTTGATTAGTGCCTACTGTCAGTATTTTATTCATCCAAATTTATTGCATTTGCATTATGGTAGCAACTTCTATATACAGGTTGTTGAAGATTTTGCGTCAGAGAATGTTGTATATGTGGAGTTGAGAACTACTCCAAAGGTAAAGAAAGGATCTCAGTCGTTAATTTTTTGTGTGCCTGTGTCCATGAATTTGTGAAGAAAATATTTGCTTAATTTCATGCAGAAAAATGAATCCAAAGGAATGAGCAAACGCTCTTATATTGAAGCTGTACTTGAAGGTCTAAGATCTGTCAGTTCGGTTGATCTGGGTTTTATTCCTTTCAGCGAAGAGATTAAAAACCATTCAAACCCTATACTCTCAGTCTCAGCTACAGATGATAGAAGTAATGAAAATACTAGGAAAAAAATCTTTGTTAGGCTTCTCTTAAGCATTGACCGGAGGGAGACAACGGAAGCAGCAATGGAAACTGTGAGTGTAATACACAAATCATTTGGGCAAACTTAAGGCTGTTTGTTACTTCCTTTGAATAATCTGCTTCTTTGCTGTAGGTCATGCTTGCATTGGAAATGAGGCATTTTGGGGTTGTTGGAATTGACCTCTCTGGGAATCCAGCCGTTGGTGAATGGTATATCATTTTGACCTAGATTCCTCTATAGTATTTACTTCAAATATTAGAAGTCGATGCTTGAGCTCAAAATTCCTGGTTTATCATTTGCAGGGTTACATATTTGCCAGCACTAAAATTTGCTCGAGAACAGGGTCTTTATGTAACTCTTCACTGTGGAGAGGTGTTTTAGTATTTTCCTGTACCTGTTACTAGTGTTGTTTCACTGGCAGGTTTGTTTTTATGACTCAAATTTTTTTTGTTAGGTGCCTAACTTGAGGGAGATACATGATATGCTTGACTTTCTTCCTGAAAGGATTGGACATGCATGTTTCTTTGAGGAGGAACACTGGAGAAGGCTGAAATCGTCTAAAATTCCGGTTAGACTTGCAAGTTGAATTACCTTTAGTGACATAAATGATAAATTAAATCAAAATAGTAGTGATGTACTGTGATTTGTATGGCCACAAGAAGAATTTTTACCCGTAAATAACCAAACAATTAAACGGTTCTGCACACTCCAACCTTTATTTTGTCAATTTTGAAGTTTTATTTGATCTGGGAAAGTGGAAGTGAAACTCAATGATGTCTCATTTCTACTGATCATCCGGTGGCTAAGTTTGAATGCTTCATATGATATTGTTTCTTTCCAATTTATTAACTGCCAAGTTACATATATTCTATATCATCTTCACAATTTTCAGGTTGAACTTTGTTTGACATCAAACATTAGGACATTGTCCGTTCCATCAATTGATGCTCATCATTTTGGTAGGTTCTTTAGTTTATATTTGTTAAATTGAAATCACAAATATAGAATTAATAAAAGAACCTCATTGTATTCTCAACTTCATTCGAGATTTTAGAGTAGAAATGGTTCTGTATTTTGAAGAAAAAATGTTTCTTCTTTTTGGTTAATATAAGAGAGAATTTGTGTACAAAATACCCGTTTTCACTTTTTATGTTTACCCTCTTCAAGCTAAATTTCCTCTAAAGAACCTCACAGAACCAGAAAAGTACAATTTCTCATCGAGCGAAGTTAACCCCCCTTCTGATGTCATTCTACTCAATTGTATATTAGACTTTGATCAAGGAATAATGTTCAATCATTGTTTCTTAATATCATGCTTTTGTTGATGCAGTGGATTTGTATAATGCAAAACATCCTGTAGTCCTGTGTACTGATGACTCAGGTGTGTTCTCCACCAGTCTCTCCAACGAATACAAAATTGCTGCTTCTTCATTTGGTAAGTTTGACTTCACTCTTGCATTCAATAGACTTTAAACTAATGTATAATAAACTTTGCTGACTACTTGTTGATTGTGATATTATATAGGCCTTGGAAGGAAGGAGATGTTTGAGCTATCAAAGAATGCTGTTGAATTTATATTTGCGGATAACACGGCAAAAGAGGATTTAAGAAATATTTTTAGTTTAGCAGCAAATGATCTGGAGCTCTAG

Protein sequence

>MS.gene33527.t1

MEWCMSIPKVELHAHLNGSIRDSTLLELAKGLGDKGLIDFSQVQHVILKNDRSLSEVFKLFDVIHILTTDHATVTRITKEVVEDFASENVVYVELRTTPKKNESKGMSKRSYIEAVLEGLRSVSSVDLGFIPFSEEIKNHSNPILSVSATDDRSNENTRKKIFVRLLLSIDRRETTEAAMETVMLALEMRHFGVVGIDLSGNPAVGEWVTYLPALKFAREQGLYVTLHCGEVPNLREIHDMLDFLPERIGHACFFEEEHWRRLKSSKIPVELCLTSNIRTLSVPSIDAHHFVDLYNAKHPVVLCTDDSGVFSTSLSNEYKIAASSFGLGRKEMFELSKNAVEFIFADNTAKEDLRNIFSLAANDLEL