Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64101.t1 | XP_003615230.1 | 96.7 | 269 | 7 | 2 | 1 | 268 | 1 | 268 | 9.30E-74 | 287 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64101.t1 | Q9SB61 | 47.7 | 243 | 84 | 2 | 26 | 268 | 21 | 220 | 4.9e-39 | 162.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64101.t1 | G7KBF3 | 96.7 | 269 | 7 | 2 | 1 | 268 | 1 | 268 | 6.7e-74 | 287.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene64101.t1 | TF | zf-HD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene64101 | 0.816678 | 4.89E-52 | -1.69E-46 |
| MS.gene049260 | MS.gene64101 | 0.80308 | 4.14E-49 | -1.69E-46 |
| MS.gene049341 | MS.gene64101 | 0.844244 | 8.53E-59 | -1.69E-46 |
| MS.gene049821 | MS.gene64101 | 0.830725 | 2.50E-55 | -1.69E-46 |
| MS.gene049979 | MS.gene64101 | 0.805071 | 1.59E-49 | -1.69E-46 |
| MS.gene050020 | MS.gene64101 | 0.964797 | 8.80E-124 | -1.69E-46 |
| MS.gene05016 | MS.gene64101 | 0.803497 | 3.39E-49 | -1.69E-46 |
| MS.gene050563 | MS.gene64101 | 0.803757 | 2.99E-49 | -1.69E-46 |
| MS.gene050584 | MS.gene64101 | 0.89188 | 2.65E-74 | -1.69E-46 |
| MS.gene050585 | MS.gene64101 | 0.859833 | 3.15E-63 | -1.69E-46 |
| MS.gene050586 | MS.gene64101 | 0.856166 | 3.87E-62 | -1.69E-46 |
| MS.gene050587 | MS.gene64101 | 0.844903 | 5.67E-59 | -1.69E-46 |
| MS.gene050807 | MS.gene64101 | 0.88628 | 3.92E-72 | -1.69E-46 |
| MS.gene050808 | MS.gene64101 | 0.854443 | 1.23E-61 | -1.69E-46 |
| MS.gene050815 | MS.gene64101 | 0.828189 | 1.03E-54 | -1.69E-46 |
| MS.gene051328 | MS.gene64101 | 0.81319 | 2.90E-51 | -1.69E-46 |
| MS.gene05143 | MS.gene64101 | 0.817386 | 3.39E-52 | -1.69E-46 |
| MS.gene05157 | MS.gene64101 | 0.864353 | 1.29E-64 | -1.69E-46 |
| MS.gene051626 | MS.gene64101 | 0.809123 | 2.21E-50 | -1.69E-46 |
| MS.gene051719 | MS.gene64101 | 0.828733 | 7.63E-55 | -1.69E-46 |
| MS.gene051720 | MS.gene64101 | 0.833176 | 6.20E-56 | -1.69E-46 |
| MS.gene051789 | MS.gene64101 | 0.883914 | 3.00E-71 | -1.69E-46 |
| MS.gene052020 | MS.gene64101 | 0.80651 | 7.94E-50 | -1.69E-46 |
| MS.gene052133 | MS.gene64101 | 0.822423 | 2.39E-53 | -1.69E-46 |
| MS.gene052142 | MS.gene64101 | 0.848342 | 6.51E-60 | -1.69E-46 |
| MS.gene052323 | MS.gene64101 | 0.802252 | 6.13E-49 | -1.69E-46 |
| MS.gene05236 | MS.gene64101 | 0.818227 | 2.19E-52 | -1.69E-46 |
| MS.gene052516 | MS.gene64101 | 0.807939 | 3.96E-50 | -1.69E-46 |
| MS.gene052611 | MS.gene64101 | 0.822483 | 2.31E-53 | -1.69E-46 |
| MS.gene052612 | MS.gene64101 | 0.800684 | 1.28E-48 | -1.69E-46 |
| MS.gene052665 | MS.gene64101 | 0.861034 | 1.36E-63 | -1.69E-46 |
| MS.gene052879 | MS.gene64101 | 0.809077 | 2.26E-50 | -1.69E-46 |
| MS.gene053074 | MS.gene64101 | 0.86495 | 8.41E-65 | -1.69E-46 |
| MS.gene053174 | MS.gene64101 | 0.820406 | 6.98E-53 | -1.69E-46 |
| MS.gene053383 | MS.gene64101 | 0.849159 | 3.87E-60 | -1.69E-46 |
| MS.gene053563 | MS.gene64101 | 0.851318 | 9.59E-61 | -1.69E-46 |
| MS.gene053581 | MS.gene64101 | 0.811927 | 5.48E-51 | -1.69E-46 |
| MS.gene053630 | MS.gene64101 | 0.802505 | 5.43E-49 | -1.69E-46 |
| MS.gene053894 | MS.gene64101 | 0.817427 | 3.32E-52 | -1.69E-46 |
| MS.gene054550 | MS.gene64101 | 0.819064 | 1.41E-52 | -1.69E-46 |
| MS.gene05456 | MS.gene64101 | 0.878154 | 3.53E-69 | -1.69E-46 |
| MS.gene054596 | MS.gene64101 | 0.808197 | 3.49E-50 | -1.69E-46 |
| MS.gene054697 | MS.gene64101 | 0.800351 | 1.50E-48 | -1.69E-46 |
| MS.gene054791 | MS.gene64101 | 0.830764 | 2.44E-55 | -1.69E-46 |
| MS.gene054833 | MS.gene64101 | 0.805583 | 1.24E-49 | -1.69E-46 |
| MS.gene055207 | MS.gene64101 | 0.802102 | 6.58E-49 | -1.69E-46 |
| MS.gene055357 | MS.gene64101 | 0.801509 | 8.71E-49 | -1.69E-46 |
| MS.gene055359 | MS.gene64101 | 0.843513 | 1.34E-58 | -1.69E-46 |
| MS.gene055465 | MS.gene64101 | 0.817848 | 2.67E-52 | -1.69E-46 |
| MS.gene055466 | MS.gene64101 | 0.825665 | 4.14E-54 | -1.69E-46 |
| MS.gene055468 | MS.gene64101 | 0.802004 | 6.89E-49 | -1.69E-46 |
| MS.gene055512 | MS.gene64101 | 0.826674 | 2.38E-54 | -1.69E-46 |
| MS.gene055890 | MS.gene64101 | 0.818349 | 2.05E-52 | -1.69E-46 |
| MS.gene055891 | MS.gene64101 | 0.804107 | 2.53E-49 | -1.69E-46 |
| MS.gene055912 | MS.gene64101 | 0.815054 | 1.12E-51 | -1.69E-46 |
| MS.gene056005 | MS.gene64101 | 0.817343 | 3.46E-52 | -1.69E-46 |
| MS.gene056553 | MS.gene64101 | 0.812631 | 3.85E-51 | -1.69E-46 |
| MS.gene056589 | MS.gene64101 | 0.802224 | 6.21E-49 | -1.69E-46 |
| MS.gene056643 | MS.gene64101 | 0.834662 | 2.63E-56 | -1.69E-46 |
| MS.gene057060 | MS.gene64101 | 0.835132 | 2.01E-56 | -1.69E-46 |
| MS.gene057444 | MS.gene64101 | 0.804135 | 2.50E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene64101 | 0.821122 | 4.78E-53 | -1.69E-46 |
| MS.gene059006 | MS.gene64101 | 0.89881 | 3.68E-77 | -1.69E-46 |
| MS.gene059607 | MS.gene64101 | 0.813334 | 2.70E-51 | -1.69E-46 |
| MS.gene05978 | MS.gene64101 | 0.82214 | 2.78E-53 | -1.69E-46 |
| MS.gene059937 | MS.gene64101 | 0.834172 | 3.50E-56 | -1.69E-46 |
| MS.gene060137 | MS.gene64101 | -0.813379 | 2.64E-51 | -1.69E-46 |
| MS.gene060470 | MS.gene64101 | 0.839083 | 1.96E-57 | -1.69E-46 |
| MS.gene060587 | MS.gene64101 | 0.850854 | 1.30E-60 | -1.69E-46 |
| MS.gene060691 | MS.gene64101 | 0.814231 | 1.71E-51 | -1.69E-46 |
| MS.gene060803 | MS.gene64101 | 0.811941 | 5.44E-51 | -1.69E-46 |
| MS.gene061176 | MS.gene64101 | 0.854591 | 1.11E-61 | -1.69E-46 |
| MS.gene061177 | MS.gene64101 | 0.853829 | 1.84E-61 | -1.69E-46 |
| MS.gene061178 | MS.gene64101 | 0.854453 | 1.22E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64101.t1 | MTR_5g065440 | 97.026 | 269 | 6 | 2 | 1 | 268 | 1 | 268 | 0.0 | 537 |
| MS.gene64101.t1 | MTR_3g100650 | 59.589 | 292 | 76 | 7 | 1 | 268 | 1 | 274 | 1.67e-96 | 285 |
| MS.gene64101.t1 | MTR_5g032600 | 52.055 | 219 | 74 | 7 | 58 | 268 | 77 | 272 | 4.02e-56 | 181 |
| MS.gene64101.t1 | MTR_4g134580 | 42.169 | 249 | 91 | 6 | 58 | 267 | 112 | 346 | 2.89e-51 | 172 |
| MS.gene64101.t1 | MTR_1g077020 | 41.315 | 213 | 92 | 5 | 56 | 267 | 6 | 186 | 1.21e-47 | 157 |
| MS.gene64101.t1 | MTR_2g076780 | 40.807 | 223 | 95 | 8 | 59 | 267 | 37 | 236 | 2.44e-45 | 152 |
| MS.gene64101.t1 | MTR_7g010300 | 40.541 | 222 | 98 | 6 | 56 | 267 | 85 | 282 | 2.11e-44 | 153 |
| MS.gene64101.t1 | MTR_5g020510 | 39.819 | 221 | 107 | 6 | 54 | 267 | 94 | 295 | 4.16e-44 | 151 |
| MS.gene64101.t1 | MTR_8g069080 | 41.667 | 216 | 104 | 7 | 58 | 267 | 68 | 267 | 1.41e-42 | 146 |
| MS.gene64101.t1 | MTR_6g012280 | 42.654 | 211 | 102 | 5 | 59 | 267 | 91 | 284 | 1.53e-40 | 143 |
| MS.gene64101.t1 | MTR_7g070643 | 80.263 | 76 | 12 | 1 | 22 | 97 | 48 | 120 | 2.61e-35 | 125 |
| MS.gene64101.t1 | MTR_6g080300 | 86.885 | 61 | 8 | 0 | 37 | 97 | 32 | 92 | 2.32e-31 | 112 |
| MS.gene64101.t1 | MTR_8g069380 | 54.795 | 73 | 33 | 0 | 196 | 268 | 43 | 115 | 7.29e-22 | 88.2 |
| MS.gene64101.t1 | MTR_8g051480 | 54.667 | 75 | 33 | 1 | 193 | 267 | 55 | 128 | 1.87e-20 | 84.7 |
| MS.gene64101.t1 | MTR_3g039810 | 57.534 | 73 | 24 | 2 | 57 | 129 | 18 | 83 | 2.28e-20 | 83.2 |
| MS.gene64101.t1 | MTR_6g073013 | 53.333 | 60 | 27 | 1 | 205 | 263 | 101 | 160 | 1.99e-16 | 75.5 |
| MS.gene64101.t1 | MTR_6g073013 | 56.000 | 50 | 21 | 1 | 59 | 107 | 19 | 68 | 1.89e-11 | 61.6 |
| MS.gene64101.t1 | MTR_6g012220 | 56.604 | 53 | 19 | 1 | 55 | 107 | 17 | 65 | 7.09e-13 | 63.2 |
| MS.gene64101.t1 | MTR_2g007810 | 100.000 | 29 | 0 | 0 | 69 | 97 | 19 | 47 | 8.04e-13 | 63.2 |
| MS.gene64101.t1 | MTR_8g467340 | 54.545 | 55 | 23 | 1 | 56 | 108 | 61 | 115 | 1.46e-12 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64101.t1 | AT4G24660 | 48.106 | 264 | 88 | 7 | 7 | 268 | 4 | 220 | 3.12e-65 | 203 |
| MS.gene64101.t1 | AT4G24660 | 47.710 | 262 | 84 | 8 | 7 | 264 | 4 | 216 | 5.23e-62 | 195 |
| MS.gene64101.t1 | AT2G02540 | 38.868 | 265 | 124 | 7 | 18 | 267 | 43 | 284 | 3.34e-58 | 188 |
| MS.gene64101.t1 | AT2G02540 | 38.868 | 265 | 124 | 7 | 18 | 267 | 43 | 284 | 3.34e-58 | 188 |
| MS.gene64101.t1 | AT1G14440 | 40.654 | 214 | 103 | 4 | 57 | 267 | 88 | 280 | 7.00e-52 | 172 |
| MS.gene64101.t1 | AT1G14440 | 40.654 | 214 | 103 | 4 | 57 | 267 | 88 | 280 | 7.00e-52 | 172 |
| MS.gene64101.t1 | AT5G65410 | 43.243 | 222 | 90 | 3 | 50 | 268 | 66 | 254 | 5.12e-50 | 166 |
| MS.gene64101.t1 | AT2G18350 | 41.784 | 213 | 90 | 4 | 57 | 267 | 80 | 260 | 2.22e-48 | 161 |
| MS.gene64101.t1 | AT1G75240 | 41.126 | 231 | 118 | 2 | 56 | 268 | 73 | 303 | 7.10e-47 | 159 |
| MS.gene64101.t1 | AT3G50890 | 41.038 | 212 | 94 | 3 | 58 | 266 | 59 | 242 | 1.42e-43 | 148 |
| MS.gene64101.t1 | AT1G69600 | 40.187 | 214 | 95 | 5 | 57 | 264 | 29 | 215 | 8.42e-38 | 133 |
| MS.gene64101.t1 | AT5G15210 | 36.073 | 219 | 95 | 5 | 54 | 261 | 51 | 235 | 2.48e-36 | 130 |
| MS.gene64101.t1 | AT5G39760 | 37.441 | 211 | 126 | 3 | 59 | 267 | 56 | 262 | 2.52e-35 | 129 |
| MS.gene64101.t1 | AT3G28920 | 36.744 | 215 | 116 | 4 | 57 | 264 | 50 | 251 | 4.16e-35 | 128 |
| MS.gene64101.t1 | AT5G60480 | 37.981 | 208 | 89 | 7 | 56 | 261 | 34 | 203 | 1.34e-30 | 114 |
| MS.gene64101.t1 | AT1G74660 | 67.647 | 68 | 20 | 1 | 55 | 122 | 35 | 100 | 1.53e-24 | 94.7 |
| MS.gene64101.t1 | AT1G74660 | 70.492 | 61 | 18 | 0 | 55 | 115 | 35 | 95 | 1.64e-24 | 94.7 |
| MS.gene64101.t1 | AT3G28917 | 65.217 | 69 | 21 | 2 | 56 | 123 | 30 | 96 | 1.37e-23 | 92.4 |
| MS.gene64101.t1 | AT1G18835 | 63.793 | 58 | 18 | 1 | 57 | 114 | 24 | 78 | 5.52e-19 | 79.7 |
| MS.gene64101.t1 | AT5G42780 | 36.232 | 69 | 44 | 0 | 196 | 264 | 170 | 238 | 7.15e-11 | 61.2 |
| MS.gene64101.t1 | AT5G42780 | 57.692 | 52 | 18 | 2 | 59 | 109 | 64 | 112 | 7.51e-11 | 61.2 |
Find 79 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCACTGGTGTTTGTGTTTC+TGG | 0.083748 | 5.1:-50377164 | None:intergenic |
| AGAGAAATGTTCTTAAAGTT+TGG | 0.256503 | 5.1:+50377864 | MS.gene64101:CDS |
| GGTGGTGGTGGTAGTGGTGG+TGG | 0.308191 | 5.1:+50377701 | MS.gene64101:CDS |
| GCTCTCCCTGCTGCTGCTTC+CGG | 0.319955 | 5.1:+50377617 | MS.gene64101:CDS |
| CCTGCTGCTGCTTCCGGTGG+TGG | 0.355404 | 5.1:+50377623 | MS.gene64101:CDS |
| TCTCTTTGCGGTGGAAGTTC+CGG | 0.377003 | 5.1:-50377421 | None:intergenic |
| GTGGAGGTGGTGGCTGTGAC+CGG | 0.391995 | 5.1:-50377472 | None:intergenic |
| GGTGGAGGTGGTGGTGGTGG+TGG | 0.402507 | 5.1:+50377689 | MS.gene64101:CDS |
| TGGTATTGCGGCGGTGGAGG+TGG | 0.407593 | 5.1:-50377485 | None:intergenic |
| GGTGGTGGTAGTGGTGGTGG+TGG | 0.411629 | 5.1:+50377704 | MS.gene64101:CDS |
| CCACCACCGGAAGCAGCAGC+AGG | 0.427582 | 5.1:-50377623 | None:intergenic |
| TGATGGTATTGCGGCGGTGG+AGG | 0.428238 | 5.1:-50377488 | None:intergenic |
| AAGAATCACGCCGTTAGCTT+CGG | 0.428386 | 5.1:+50377308 | MS.gene64101:CDS |
| TTTATCGCTGCCGGTGAAGA+AGG | 0.429195 | 5.1:+50377359 | MS.gene64101:CDS |
| TGGTGATGGTATTGCGGCGG+TGG | 0.436665 | 5.1:-50377491 | None:intergenic |
| AGTAGCGGTGGAGGTGGTGG+TGG | 0.442408 | 5.1:+50377683 | MS.gene64101:CDS |
| TATTGCGGCGGTGGAGGTGG+TGG | 0.444382 | 5.1:-50377482 | None:intergenic |
| AGCAGTAGCGGTGGAGGTGG+TGG | 0.444551 | 5.1:+50377680 | MS.gene64101:CDS |
| GTAGTGGTGGTGGTGGATCA+AGG | 0.444962 | 5.1:+50377711 | MS.gene64101:CDS |
| TTCGGTGGCCATGCTGTTGA+CGG | 0.447816 | 5.1:+50377326 | MS.gene64101:CDS |
| AGCGGTGGAGGTGGTGGTGG+TGG | 0.451872 | 5.1:+50377686 | MS.gene64101:CDS |
| CACAATAACAAGAACACTCT+TGG | 0.453033 | 5.1:+50377890 | MS.gene64101:CDS |
| GGAGGAGCACGGTGGTAGTA+AGG | 0.453304 | 5.1:-50377530 | None:intergenic |
| GGAACAGAAAGAGAAATTGT+TGG | 0.455764 | 5.1:+50377760 | MS.gene64101:CDS |
| CGTGATTCTTCTGACATTCT+CGG | 0.457636 | 5.1:-50377295 | None:intergenic |
| AAACGGTCAGTTCATGTAAC+CGG | 0.476371 | 5.1:+50377453 | MS.gene64101:CDS |
| AAACTCACCGTTGGAGTTGA+CGG | 0.483943 | 5.1:+50377227 | MS.gene64101:CDS |
| CCACCTCCACCGCTACTGCT+AGG | 0.486199 | 5.1:-50377677 | None:intergenic |
| TTTCACCATCGATCTCTTTG+CGG | 0.492117 | 5.1:-50377433 | None:intergenic |
| AACGGTCTGTGATGTGCCAC+CGG | 0.497928 | 5.1:-50377593 | None:intergenic |
| CAAAGAGATCGATGGTGAAA+CGG | 0.500230 | 5.1:+50377436 | MS.gene64101:CDS |
| ATGATGGTGGTGAAGGTAAC+CGG | 0.500422 | 5.1:-50377561 | None:intergenic |
| AGAGGAACAAGAAGAGGAGA+TGG | 0.506205 | 5.1:+50377136 | MS.gene64101:CDS |
| TTCAAGTGTTCCTTCTTCAC+CGG | 0.506613 | 5.1:-50377369 | None:intergenic |
| CGTGCTCCTCCTTCCACAGC+CGG | 0.511664 | 5.1:+50377542 | MS.gene64101:CDS |
| TTCTCTGCTCATTCCACCAC+CGG | 0.513977 | 5.1:-50377636 | None:intergenic |
| CACCTCCACCGCTACTGCTA+GGG | 0.520409 | 5.1:-50377676 | None:intergenic |
| AACCCTAGCAGTAGCGGTGG+AGG | 0.520715 | 5.1:+50377674 | MS.gene64101:CDS |
| CTCCCTGCTGCTGCTTCCGG+TGG | 0.523308 | 5.1:+50377620 | MS.gene64101:CDS |
| GCAGCAGCAGGGAGAGCCAA+CGG | 0.524507 | 5.1:-50377611 | None:intergenic |
| CCTAGCAGTAGCGGTGGAGG+TGG | 0.532726 | 5.1:+50377677 | MS.gene64101:CDS |
| AATCACGCCGTTAGCTTCGG+TGG | 0.536570 | 5.1:+50377311 | MS.gene64101:CDS |
| GAGTCATAACTCGTTGGCAC+TGG | 0.537593 | 5.1:-50377179 | None:intergenic |
| CCACCATCATCTTGCAACTC+CGG | 0.539922 | 5.1:+50377574 | MS.gene64101:CDS |
| GCGCAGCTTGTAACTGTCAC+CGG | 0.541516 | 5.1:+50377402 | MS.gene64101:CDS |
| GTGGTGAAGGTAACCGGCTG+TGG | 0.551358 | 5.1:-50377555 | None:intergenic |
| TTGCAAGATGATGGTGGTGA+AGG | 0.558218 | 5.1:-50377568 | None:intergenic |
| CACCATCGATCTCTTTGCGG+TGG | 0.563677 | 5.1:-50377430 | None:intergenic |
| TGTTGCGAGTTTATCGCTGC+CGG | 0.567002 | 5.1:+50377350 | MS.gene64101:CDS |
| CCACCGGAGTTGCAAGATGA+TGG | 0.569041 | 5.1:-50377577 | None:intergenic |
| TGAAGGTAACCGGCTGTGGA+AGG | 0.577589 | 5.1:-50377551 | None:intergenic |
| TCACGTTCAAAACTCACCGT+TGG | 0.581295 | 5.1:+50377218 | MS.gene64101:CDS |
| AGAATTGATTGCTGTGGTGA+TGG | 0.582941 | 5.1:-50377505 | None:intergenic |
| AATGGAATTTGATGAACAAG+AGG | 0.584250 | 5.1:+50377118 | None:intergenic |
| ATCTCAAACCCTAGCAGTAG+CGG | 0.586840 | 5.1:+50377668 | MS.gene64101:CDS |
| TTTCCTTCCGTCAACTCCAA+CGG | 0.588914 | 5.1:-50377234 | None:intergenic |
| TGAACAAGAGGAACAAGAAG+AGG | 0.593455 | 5.1:+50377130 | MS.gene64101:CDS |
| CACCACCGGAAGCAGCAGCA+GGG | 0.602778 | 5.1:-50377622 | None:intergenic |
| GTTGGAGTTGACGGAAGGAA+AGG | 0.610107 | 5.1:+50377236 | MS.gene64101:CDS |
| CCAACGAGTTATGACTCACT+TGG | 0.611073 | 5.1:+50377185 | MS.gene64101:CDS |
| CGCAACAACCGTCAACAGCA+TGG | 0.612162 | 5.1:-50377334 | None:intergenic |
| AGGTAACCGGCTGTGGAAGG+AGG | 0.612370 | 5.1:-50377548 | None:intergenic |
| GGTGGCACATCACAGACCGT+TGG | 0.612785 | 5.1:+50377595 | MS.gene64101:CDS |
| ATTCAGAACAAAATTCACTC+AGG | 0.624697 | 5.1:+50377739 | MS.gene64101:CDS |
| TTGCTGTGGTGATGGTATTG+CGG | 0.630881 | 5.1:-50377497 | None:intergenic |
| TCAAACCCTAGCAGTAGCGG+TGG | 0.634884 | 5.1:+50377671 | MS.gene64101:CDS |
| GGAAGGAAAGGTAACGGCAA+CGG | 0.639375 | 5.1:+50377248 | MS.gene64101:CDS |
| TCACCGTTGGAGTTGACGGA+AGG | 0.640977 | 5.1:+50377231 | MS.gene64101:CDS |
| CCATCATCTTGCAACTCCGG+TGG | 0.641524 | 5.1:+50377577 | MS.gene64101:CDS |
| CCAAGTGAGTCATAACTCGT+TGG | 0.644449 | 5.1:-50377185 | None:intergenic |
| TTCCACCGCAAAGAGATCGA+TGG | 0.654798 | 5.1:+50377428 | MS.gene64101:CDS |
| GTTGACGGAAGGAAAGGTAA+CGG | 0.669388 | 5.1:+50377242 | MS.gene64101:CDS |
| CGGCTGTGGAAGGAGGAGCA+CGG | 0.673027 | 5.1:-50377541 | None:intergenic |
| AGCATGGCCACCGAAGCTAA+CGG | 0.680090 | 5.1:-50377318 | None:intergenic |
| CTGTGGTGATGGTATTGCGG+CGG | 0.691893 | 5.1:-50377494 | None:intergenic |
| CCGGAGTTGCAAGATGATGG+TGG | 0.694873 | 5.1:-50377574 | None:intergenic |
| AGGAAAGGTAACGGCAACGG+CGG | 0.697533 | 5.1:+50377251 | MS.gene64101:CDS |
| AAGGCGAGAATTGATTGCTG+TGG | 0.722344 | 5.1:-50377511 | None:intergenic |
| CTGTGGAAGGAGGAGCACGG+TGG | 0.754237 | 5.1:-50377538 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAGAAATGTTCTTAAAGTT+TGG | + | chr5.1:50377864-50377883 | MS.gene64101:CDS | 25.0% |
| ATTCAGAACAAAATTCACTC+AGG | + | chr5.1:50377739-50377758 | MS.gene64101:CDS | 30.0% | |
| CACAATAACAAGAACACTCT+TGG | + | chr5.1:50377890-50377909 | MS.gene64101:CDS | 35.0% | |
| GGAACAGAAAGAGAAATTGT+TGG | + | chr5.1:50377760-50377779 | MS.gene64101:CDS | 35.0% | |
| AAACGGTCAGTTCATGTAAC+CGG | + | chr5.1:50377453-50377472 | MS.gene64101:CDS | 40.0% | |
| AGAATTGATTGCTGTGGTGA+TGG | - | chr5.1:50377508-50377527 | None:intergenic | 40.0% | |
| CAAAGAGATCGATGGTGAAA+CGG | + | chr5.1:50377436-50377455 | MS.gene64101:CDS | 40.0% | |
| CGTGATTCTTCTGACATTCT+CGG | - | chr5.1:50377298-50377317 | None:intergenic | 40.0% | |
| TGAACAAGAGGAACAAGAAG+AGG | + | chr5.1:50377130-50377149 | MS.gene64101:CDS | 40.0% | |
| TTCAAGTGTTCCTTCTTCAC+CGG | - | chr5.1:50377372-50377391 | None:intergenic | 40.0% | |
| TTTCACCATCGATCTCTTTG+CGG | - | chr5.1:50377436-50377455 | None:intergenic | 40.0% | |
| ! | CTTTTGCTGAAGAACATGGT+TGG | + | chr5.1:50377783-50377802 | MS.gene64101:CDS | 40.0% |
| !! | TTGGCTTTTGCTGAAGAACA+TGG | + | chr5.1:50377779-50377798 | MS.gene64101:CDS | 40.0% |
| AAACTCACCGTTGGAGTTGA+CGG | + | chr5.1:50377227-50377246 | MS.gene64101:CDS | 45.0% | |
| AAGGCGAGAATTGATTGCTG+TGG | - | chr5.1:50377514-50377533 | None:intergenic | 45.0% | |
| AGAGGAACAAGAAGAGGAGA+TGG | + | chr5.1:50377136-50377155 | MS.gene64101:CDS | 45.0% | |
| ATCTCAAACCCTAGCAGTAG+CGG | + | chr5.1:50377668-50377687 | MS.gene64101:CDS | 45.0% | |
| CCAACGAGTTATGACTCACT+TGG | + | chr5.1:50377185-50377204 | MS.gene64101:CDS | 45.0% | |
| CCAAGTGAGTCATAACTCGT+TGG | - | chr5.1:50377188-50377207 | None:intergenic | 45.0% | |
| GTTGACGGAAGGAAAGGTAA+CGG | + | chr5.1:50377242-50377261 | MS.gene64101:CDS | 45.0% | |
| TCACGTTCAAAACTCACCGT+TGG | + | chr5.1:50377218-50377237 | MS.gene64101:CDS | 45.0% | |
| TTTCCTTCCGTCAACTCCAA+CGG | - | chr5.1:50377237-50377256 | None:intergenic | 45.0% | |
| ! | AAGAATCACGCCGTTAGCTT+CGG | + | chr5.1:50377308-50377327 | MS.gene64101:CDS | 45.0% |
| !! | ATGATGGTGGTGAAGGTAAC+CGG | - | chr5.1:50377564-50377583 | None:intergenic | 45.0% |
| !! | TTGCAAGATGATGGTGGTGA+AGG | - | chr5.1:50377571-50377590 | None:intergenic | 45.0% |
| !! | TTGCTGTGGTGATGGTATTG+CGG | - | chr5.1:50377500-50377519 | None:intergenic | 45.0% |
| CCACCATCATCTTGCAACTC+CGG | + | chr5.1:50377574-50377593 | MS.gene64101:CDS | 50.0% | |
| GAGTCATAACTCGTTGGCAC+TGG | - | chr5.1:50377182-50377201 | None:intergenic | 50.0% | |
| GTTGGAGTTGACGGAAGGAA+AGG | + | chr5.1:50377236-50377255 | MS.gene64101:CDS | 50.0% | |
| TCTCTTTGCGGTGGAAGTTC+CGG | - | chr5.1:50377424-50377443 | None:intergenic | 50.0% | |
| TGTTGCGAGTTTATCGCTGC+CGG | + | chr5.1:50377350-50377369 | MS.gene64101:CDS | 50.0% | |
| TTCCACCGCAAAGAGATCGA+TGG | + | chr5.1:50377428-50377447 | MS.gene64101:CDS | 50.0% | |
| TTCTCTGCTCATTCCACCAC+CGG | - | chr5.1:50377639-50377658 | None:intergenic | 50.0% | |
| TTTATCGCTGCCGGTGAAGA+AGG | + | chr5.1:50377359-50377378 | MS.gene64101:CDS | 50.0% | |
| ! | GGAAGGAAAGGTAACGGCAA+CGG | + | chr5.1:50377248-50377267 | MS.gene64101:CDS | 50.0% |
| !! | GGCACTGGTGTTTGTGTTTC+TGG | - | chr5.1:50377167-50377186 | None:intergenic | 50.0% |
| AACGGTCTGTGATGTGCCAC+CGG | - | chr5.1:50377596-50377615 | None:intergenic | 55.0% | |
| AGCATGGCCACCGAAGCTAA+CGG | - | chr5.1:50377321-50377340 | None:intergenic | 55.0% | |
| CACCATCGATCTCTTTGCGG+TGG | - | chr5.1:50377433-50377452 | None:intergenic | 55.0% | |
| CCACCGGAGTTGCAAGATGA+TGG | - | chr5.1:50377580-50377599 | None:intergenic | 55.0% | |
| CCATCATCTTGCAACTCCGG+TGG | + | chr5.1:50377577-50377596 | MS.gene64101:CDS | 55.0% | |
| CGCAACAACCGTCAACAGCA+TGG | - | chr5.1:50377337-50377356 | None:intergenic | 55.0% | |
| GCGCAGCTTGTAACTGTCAC+CGG | + | chr5.1:50377402-50377421 | MS.gene64101:CDS | 55.0% | |
| TCAAACCCTAGCAGTAGCGG+TGG | + | chr5.1:50377671-50377690 | MS.gene64101:CDS | 55.0% | |
| TCACCGTTGGAGTTGACGGA+AGG | + | chr5.1:50377231-50377250 | MS.gene64101:CDS | 55.0% | |
| TGAAGGTAACCGGCTGTGGA+AGG | - | chr5.1:50377554-50377573 | None:intergenic | 55.0% | |
| TTCGGTGGCCATGCTGTTGA+CGG | + | chr5.1:50377326-50377345 | MS.gene64101:CDS | 55.0% | |
| ! | AATCACGCCGTTAGCTTCGG+TGG | + | chr5.1:50377311-50377330 | MS.gene64101:CDS | 55.0% |
| ! | AGGAAAGGTAACGGCAACGG+CGG | + | chr5.1:50377251-50377270 | MS.gene64101:CDS | 55.0% |
| ! | CAACGGCGGTTTTGCTTCGA+CGG | + | chr5.1:50377265-50377284 | MS.gene64101:CDS | 55.0% |
| ! | CCGGAGTTGCAAGATGATGG+TGG | - | chr5.1:50377577-50377596 | None:intergenic | 55.0% |
| !! | CTGTGGTGATGGTATTGCGG+CGG | - | chr5.1:50377497-50377516 | None:intergenic | 55.0% |
| !! | GTAGTGGTGGTGGTGGATCA+AGG | + | chr5.1:50377711-50377730 | MS.gene64101:CDS | 55.0% |
| AACCCTAGCAGTAGCGGTGG+AGG | + | chr5.1:50377674-50377693 | MS.gene64101:CDS | 60.0% | |
| AGGTAACCGGCTGTGGAAGG+AGG | - | chr5.1:50377551-50377570 | None:intergenic | 60.0% | |
| CACCTCCACCGCTACTGCTA+GGG | - | chr5.1:50377679-50377698 | None:intergenic | 60.0% | |
| GGTGGCACATCACAGACCGT+TGG | + | chr5.1:50377595-50377614 | MS.gene64101:CDS | 60.0% | |
| GTGGTGAAGGTAACCGGCTG+TGG | - | chr5.1:50377558-50377577 | None:intergenic | 60.0% | |
| TGATGGTATTGCGGCGGTGG+AGG | - | chr5.1:50377491-50377510 | None:intergenic | 60.0% | |
| ! | GCGGTTTTGCTTCGACGGTG+AGG | + | chr5.1:50377270-50377289 | MS.gene64101:CDS | 60.0% |
| ! | GGAGGAGCACGGTGGTAGTA+AGG | - | chr5.1:50377533-50377552 | None:intergenic | 60.0% |
| !! | TGGTGATGGTATTGCGGCGG+TGG | - | chr5.1:50377494-50377513 | None:intergenic | 60.0% |
| AGCAGTAGCGGTGGAGGTGG+TGG | + | chr5.1:50377680-50377699 | MS.gene64101:CDS | 65.0% | |
| CACCACCGGAAGCAGCAGCA+GGG | - | chr5.1:50377625-50377644 | None:intergenic | 65.0% | |
| CCACCTCCACCGCTACTGCT+AGG | - | chr5.1:50377680-50377699 | None:intergenic | 65.0% | |
| CCTAGCAGTAGCGGTGGAGG+TGG | + | chr5.1:50377677-50377696 | MS.gene64101:CDS | 65.0% | |
| CGGCTGTGGAAGGAGGAGCA+CGG | - | chr5.1:50377544-50377563 | None:intergenic | 65.0% | |
| CGTGCTCCTCCTTCCACAGC+CGG | + | chr5.1:50377542-50377561 | MS.gene64101:CDS | 65.0% | |
| GCAGCAGCAGGGAGAGCCAA+CGG | - | chr5.1:50377614-50377633 | None:intergenic | 65.0% | |
| TATTGCGGCGGTGGAGGTGG+TGG | - | chr5.1:50377485-50377504 | None:intergenic | 65.0% | |
| TGGTATTGCGGCGGTGGAGG+TGG | - | chr5.1:50377488-50377507 | None:intergenic | 65.0% | |
| ! | AGTAGCGGTGGAGGTGGTGG+TGG | + | chr5.1:50377683-50377702 | MS.gene64101:CDS | 65.0% |
| ! | CTGTGGAAGGAGGAGCACGG+TGG | - | chr5.1:50377541-50377560 | None:intergenic | 65.0% |
| ! | GCTCTCCCTGCTGCTGCTTC+CGG | + | chr5.1:50377617-50377636 | MS.gene64101:CDS | 65.0% |
| ! | GTGGAGGTGGTGGCTGTGAC+CGG | - | chr5.1:50377475-50377494 | None:intergenic | 65.0% |
| !! | GGTGGTGGTAGTGGTGGTGG+TGG | + | chr5.1:50377704-50377723 | MS.gene64101:CDS | 65.0% |
| !! | GGTGGTGGTGGTAGTGGTGG+TGG | + | chr5.1:50377701-50377720 | MS.gene64101:CDS | 65.0% |
| !! | GGTGGTGGTGGTGGTAGTGG+TGG | + | chr5.1:50377698-50377717 | MS.gene64101:CDS | 65.0% |
| !! | GGTGGTGGTGGTGGTGGTAG+TGG | + | chr5.1:50377695-50377714 | MS.gene64101:CDS | 65.0% |
| CCACCACCGGAAGCAGCAGC+AGG | - | chr5.1:50377626-50377645 | None:intergenic | 70.0% | |
| ! | CCTGCTGCTGCTTCCGGTGG+TGG | + | chr5.1:50377623-50377642 | MS.gene64101:CDS | 70.0% |
| ! | CTCCCTGCTGCTGCTTCCGG+TGG | + | chr5.1:50377620-50377639 | MS.gene64101:CDS | 70.0% |
| !! | AGCGGTGGAGGTGGTGGTGG+TGG | + | chr5.1:50377686-50377705 | MS.gene64101:CDS | 70.0% |
| !! | GGTGGAGGTGGTGGTGGTGG+TGG | + | chr5.1:50377689-50377708 | MS.gene64101:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 50377119 | 50377925 | 50377119 | ID=MS.gene64101 |
| chr5.1 | mRNA | 50377119 | 50377925 | 50377119 | ID=MS.gene64101.t1;Parent=MS.gene64101 |
| chr5.1 | exon | 50377119 | 50377925 | 50377119 | ID=MS.gene64101.t1.exon1;Parent=MS.gene64101.t1 |
| chr5.1 | CDS | 50377119 | 50377925 | 50377119 | ID=cds.MS.gene64101.t1;Parent=MS.gene64101.t1 |
| Gene Sequence |
| Protein sequence |
