AlfalfaGEDB | MS.gene77579

M. sativa cultivar XinJiangDaYe / MS.gene77579

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77579.t1	XP_024635448.1	86.1	144	13	2	1	143	48	185	8.00E-56	226.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77579.t1	G7JBF3	86.1	144	13	2	1	143	47	184	5.8e-56	226.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049230	MS.gene77579	0.855504	6.04E-62	-1.69E-46
MS.gene049254	MS.gene77579	0.811569	6.56E-51	-1.69E-46
MS.gene049378	MS.gene77579	0.861792	8.00E-64	-1.69E-46
MS.gene049419	MS.gene77579	-0.85383	1.84E-61	-1.69E-46
MS.gene049424	MS.gene77579	0.815302	9.91E-52	-1.69E-46
MS.gene049669	MS.gene77579	0.859905	2.99E-63	-1.69E-46
MS.gene050105	MS.gene77579	0.87932	1.37E-69	-1.69E-46
MS.gene050226	MS.gene77579	0.814282	1.67E-51	-1.69E-46
MS.gene050227	MS.gene77579	0.825433	4.70E-54	-1.69E-46
MS.gene050228	MS.gene77579	0.832515	9.05E-56	-1.69E-46
MS.gene050312	MS.gene77579	0.834779	2.46E-56	-1.69E-46
MS.gene050442	MS.gene77579	0.837493	5.03E-57	-1.69E-46
MS.gene050508	MS.gene77579	0.848007	8.06E-60	-1.69E-46
MS.gene050605	MS.gene77579	0.852681	3.93E-61	-1.69E-46
MS.gene050623	MS.gene77579	-0.823378	1.43E-53	-1.69E-46
MS.gene050642	MS.gene77579	0.817924	2.56E-52	-1.69E-46
MS.gene050680	MS.gene77579	0.831289	1.82E-55	-1.69E-46
MS.gene050750	MS.gene77579	0.855379	6.56E-62	-1.69E-46
MS.gene050953	MS.gene77579	0.870312	1.60E-66	-1.69E-46
MS.gene050987	MS.gene77579	0.8013	9.61E-49	-1.69E-46
MS.gene051064	MS.gene77579	0.841908	3.57E-58	-1.69E-46
MS.gene051122	MS.gene77579	0.864969	8.29E-65	-1.69E-46
MS.gene051368	MS.gene77579	0.879472	1.21E-69	-1.69E-46
MS.gene051370	MS.gene77579	0.803196	3.91E-49	-1.69E-46
MS.gene051373	MS.gene77579	0.800444	1.44E-48	-1.69E-46
MS.gene051452	MS.gene77579	0.814784	1.29E-51	-1.69E-46
MS.gene051924	MS.gene77579	-0.820862	5.48E-53	-1.69E-46
MS.gene051926	MS.gene77579	-0.8312	1.91E-55	-1.69E-46
MS.gene051935	MS.gene77579	0.802249	6.14E-49	-1.69E-46
MS.gene051983	MS.gene77579	0.822741	2.01E-53	-1.69E-46
MS.gene052012	MS.gene77579	0.80722	5.62E-50	-1.69E-46
MS.gene052108	MS.gene77579	0.819982	8.73E-53	-1.69E-46
MS.gene05216	MS.gene77579	0.834036	3.78E-56	-1.69E-46
MS.gene052176	MS.gene77579	0.878041	3.87E-69	-1.69E-46
MS.gene052190	MS.gene77579	0.814357	1.61E-51	-1.69E-46
MS.gene052208	MS.gene77579	0.805322	1.41E-49	-1.69E-46
MS.gene052333	MS.gene77579	0.897386	1.48E-76	-1.69E-46
MS.gene052389	MS.gene77579	0.857719	1.35E-62	-1.69E-46
MS.gene052426	MS.gene77579	0.820997	5.10E-53	-1.69E-46
MS.gene052464	MS.gene77579	0.809202	2.13E-50	-1.69E-46
MS.gene052500	MS.gene77579	0.842741	2.15E-58	-1.69E-46
MS.gene052502	MS.gene77579	0.842275	2.86E-58	-1.69E-46
MS.gene052586	MS.gene77579	0.864144	1.50E-64	-1.69E-46
MS.gene052674	MS.gene77579	0.835787	1.37E-56	-1.69E-46
MS.gene052675	MS.gene77579	0.834078	3.69E-56	-1.69E-46
MS.gene052677	MS.gene77579	0.836083	1.15E-56	-1.69E-46
MS.gene052824	MS.gene77579	0.828778	7.44E-55	-1.69E-46
MS.gene052826	MS.gene77579	0.843695	1.20E-58	-1.69E-46
MS.gene052867	MS.gene77579	0.803202	3.90E-49	-1.69E-46
MS.gene052954	MS.gene77579	0.871605	6.02E-67	-1.69E-46
MS.gene052965	MS.gene77579	-0.812443	4.23E-51	-1.69E-46
MS.gene053006	MS.gene77579	0.840302	9.44E-58	-1.69E-46
MS.gene05308	MS.gene77579	0.806457	8.15E-50	-1.69E-46
MS.gene053166	MS.gene77579	0.850442	1.69E-60	-1.69E-46
MS.gene053188	MS.gene77579	0.803453	3.46E-49	-1.69E-46
MS.gene053963	MS.gene77579	0.823939	1.06E-53	-1.69E-46
MS.gene05414	MS.gene77579	0.806519	7.91E-50	-1.69E-46
MS.gene05415	MS.gene77579	0.832105	1.14E-55	-1.69E-46
MS.gene054160	MS.gene77579	0.809128	2.21E-50	-1.69E-46
MS.gene054236	MS.gene77579	0.806878	6.64E-50	-1.69E-46
MS.gene054291	MS.gene77579	-0.845052	5.17E-59	-1.69E-46
MS.gene054405	MS.gene77579	0.861297	1.13E-63	-1.69E-46
MS.gene054468	MS.gene77579	0.827938	1.19E-54	-1.69E-46
MS.gene054479	MS.gene77579	0.86625	3.27E-65	-1.69E-46
MS.gene054537	MS.gene77579	0.800788	1.22E-48	-1.69E-46
MS.gene054559	MS.gene77579	0.826228	3.04E-54	-1.69E-46
MS.gene054657	MS.gene77579	0.825349	4.92E-54	-1.69E-46
MS.gene05510	MS.gene77579	0.847688	9.87E-60	-1.69E-46
MS.gene055225	MS.gene77579	0.860151	2.52E-63	-1.69E-46
MS.gene055367	MS.gene77579	0.839982	1.14E-57	-1.69E-46
MS.gene055623	MS.gene77579	0.832596	8.64E-56	-1.69E-46
MS.gene055701	MS.gene77579	0.908337	1.91E-81	-1.69E-46
MS.gene055818	MS.gene77579	0.80667	7.35E-50	-1.69E-46
MS.gene055841	MS.gene77579	0.806362	8.54E-50	-1.69E-46
MS.gene05595	MS.gene77579	0.832717	8.06E-56	-1.69E-46
MS.gene05604	MS.gene77579	0.801697	7.97E-49	-1.69E-46
MS.gene056064	MS.gene77579	0.808034	3.78E-50	-1.69E-46
MS.gene056087	MS.gene77579	0.821832	3.27E-53	-1.69E-46
MS.gene056162	MS.gene77579	0.802132	6.48E-49	-1.69E-46
MS.gene056480	MS.gene77579	0.806275	8.90E-50	-1.69E-46
MS.gene056506	MS.gene77579	0.888044	8.37E-73	-1.69E-46
MS.gene05659	MS.gene77579	0.825377	4.85E-54	-1.69E-46
MS.gene056779	MS.gene77579	0.837542	4.89E-57	-1.69E-46
MS.gene056873	MS.gene77579	0.827562	1.46E-54	-1.69E-46
MS.gene056979	MS.gene77579	0.812245	4.67E-51	-1.69E-46
MS.gene057026	MS.gene77579	0.842426	2.60E-58	-1.69E-46
MS.gene057156	MS.gene77579	-0.88111	3.15E-70	-1.69E-46
MS.gene057208	MS.gene77579	0.836261	1.04E-56	-1.69E-46
MS.gene05761	MS.gene77579	0.841533	4.48E-58	-1.69E-46
MS.gene058146	MS.gene77579	0.824463	7.96E-54	-1.69E-46
MS.gene058223	MS.gene77579	-0.86771	1.12E-65	-1.69E-46
MS.gene058399	MS.gene77579	0.810446	1.15E-50	-1.69E-46
MS.gene058435	MS.gene77579	0.819088	1.40E-52	-1.69E-46
MS.gene058498	MS.gene77579	0.8115	6.79E-51	-1.69E-46
MS.gene058502	MS.gene77579	0.810498	1.12E-50	-1.69E-46
MS.gene058548	MS.gene77579	0.834146	3.55E-56	-1.69E-46
MS.gene058595	MS.gene77579	0.819074	1.41E-52	-1.69E-46
MS.gene058979	MS.gene77579	0.823592	1.27E-53	-1.69E-46
MS.gene059161	MS.gene77579	0.814275	1.67E-51	-1.69E-46
MS.gene059382	MS.gene77579	0.800587	1.34E-48	-1.69E-46
MS.gene059609	MS.gene77579	0.808458	3.07E-50	-1.69E-46
MS.gene059710	MS.gene77579	0.852618	4.10E-61	-1.69E-46
MS.gene05979	MS.gene77579	0.812991	3.21E-51	-1.69E-46
MS.gene059830	MS.gene77579	0.824108	9.65E-54	-1.69E-46
MS.gene060011	MS.gene77579	0.839828	1.25E-57	-1.69E-46
MS.gene060246	MS.gene77579	0.808759	2.64E-50	-1.69E-46
MS.gene060297	MS.gene77579	0.816455	5.48E-52	-1.69E-46
MS.gene060310	MS.gene77579	0.801726	7.86E-49	-1.69E-46
MS.gene060322	MS.gene77579	0.827108	1.88E-54	-1.69E-46
MS.gene060572	MS.gene77579	0.821995	3.00E-53	-1.69E-46
MS.gene060683	MS.gene77579	0.831968	1.24E-55	-1.69E-46
MS.gene060685	MS.gene77579	0.828573	8.34E-55	-1.69E-46
MS.gene060802	MS.gene77579	0.804167	2.46E-49	-1.69E-46
MS.gene060803	MS.gene77579	0.813209	2.87E-51	-1.69E-46
MS.gene060840	MS.gene77579	0.816911	4.33E-52	-1.69E-46
MS.gene060850	MS.gene77579	0.822324	2.52E-53	-1.69E-46
MS.gene060851	MS.gene77579	0.838129	3.45E-57	-1.69E-46
MS.gene060852	MS.gene77579	0.838464	2.83E-57	-1.69E-46
MS.gene060879	MS.gene77579	0.863546	2.30E-64	-1.69E-46
MS.gene060998	MS.gene77579	0.826914	2.09E-54	-1.69E-46
MS.gene061040	MS.gene77579	-0.848019	8.00E-60	-1.69E-46
MS.gene061114	MS.gene77579	-0.812114	4.99E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77579.t1	MTR_3g085060	86.111	144	13	2	1	143	47	184	1.72e-82	240

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77579.t1	AT1G27300	42.000	100	48	2	43	133	92	190	2.46e-20	82.8

Find 38 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCATTCCTTTCCGAAATT+TGG	0.127733	3.2:-73142588	None:intergenic
ATACTCCCAACGGATCTAAA+TGG	0.272675	3.2:-73142444	None:intergenic
ACATCCATGTTGGTCATATC+TGG	0.303699	3.2:-73142564	None:intergenic
GCAGAAGCCACCTGCTTTGA+AGG	0.325878	3.2:-73142219	None:intergenic
AACATGGATGTACCAAATTT+CGG	0.350139	3.2:+73142576	MS.gene77579:CDS
GATAATCAAAGAGCTGAAAA+AGG	0.382164	3.2:+73142305	MS.gene77579:CDS
TGTTTCTAGCAGCCATGGTC+AGG	0.446396	3.2:+73142488	MS.gene77579:CDS
CTTTGAAGGACTAGATGCTT+CGG	0.454664	3.2:-73142205	None:intergenic
TACTCCCAACGGATCTAAAT+GGG	0.474977	3.2:-73142443	None:intergenic
AACCACCCATTTAGATCCGT+TGG	0.484895	3.2:+73142438	MS.gene77579:CDS
TAGCAGTTGGAAATGTCTAA+AGG	0.502031	3.2:+73142162	None:intergenic
ACCACCCATTTAGATCCGTT+GGG	0.504389	3.2:+73142439	MS.gene77579:CDS
TCTGCAATTGAGAAAACAAA+AGG	0.510878	3.2:+73142237	MS.gene77579:CDS
AGCATCTAGTCCTTCAAAGC+AGG	0.533942	3.2:+73142209	MS.gene77579:CDS
TGATTGTCCTCACTGTGGCT+TGG	0.535081	3.2:+73142370	MS.gene77579:CDS
CTTAAGAGAAGGAAGAGATG+TGG	0.542676	3.2:-73142538	None:intergenic
AGGATGCATGGGAAGTGAAA+AGG	0.545731	3.2:+73142278	MS.gene77579:CDS
AAGGACTAGATGCTTCGGAT+TGG	0.550420	3.2:-73142200	None:intergenic
GAAATTTGGTACATCCATGT+TGG	0.556305	3.2:-73142574	None:intergenic
TGTCATCAGCATCCTGACCA+TGG	0.563700	3.2:-73142500	None:intergenic
CTCTTTGAACATACTCCCAA+CGG	0.567570	3.2:-73142454	None:intergenic
TGTGGCTTGGCAACTATCAG+AGG	0.572775	3.2:+73142383	MS.gene77579:CDS
AAAGGTGAAACTGTGGATGA+TGG	0.574054	3.2:+73142255	MS.gene77579:CDS
AAAGATGTTTCTAGCAGCCA+TGG	0.580571	3.2:+73142483	MS.gene77579:CDS
TGTGGATGATGGAGGATGCA+TGG	0.587950	3.2:+73142266	MS.gene77579:CDS
ATATCTGGAATCTTAAGAGA+AGG	0.599963	3.2:-73142549	None:intergenic
GTGGATGATGGAGGATGCAT+GGG	0.603480	3.2:+73142267	MS.gene77579:CDS
ATCTAGTCCTTCAAAGCAGG+TGG	0.612263	3.2:+73142212	MS.gene77579:CDS
GGATGTACCAAATTTCGGAA+AGG	0.619495	3.2:+73142581	MS.gene77579:CDS
CTTGTGATGAGAGTGAAGGA+TGG	0.629930	3.2:+73142411	MS.gene77579:CDS
AGCAATGATTGTCCTCACTG+TGG	0.630188	3.2:+73142365	MS.gene77579:CDS
GGTGAAACTGTGGATGATGG+AGG	0.632117	3.2:+73142258	MS.gene77579:CDS
GATTCCAGATATGACCAACA+TGG	0.664463	3.2:+73142560	MS.gene77579:CDS
TCCCAACGGATCTAAATGGG+TGG	0.668953	3.2:-73142440	None:intergenic
GAAAACAAAAGGTGAAACTG+TGG	0.678024	3.2:+73142248	MS.gene77579:CDS
TTCACTTGTGATGAGAGTGA+AGG	0.685850	3.2:+73142407	MS.gene77579:CDS
CAGTTGGAAATGTCTAAAGG+AGG	0.689175	3.2:+73142165	None:intergenic
ATAGTTGCCAAGCCACAGTG+AGG	0.743956	3.2:-73142377	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AACATGGATGTACCAAATTT+CGG	+	chr3.2:73142576-73142595	MS.gene77579:CDS	30.0%
	GATAATCAAAGAGCTGAAAA+AGG	+	chr3.2:73142305-73142324	MS.gene77579:CDS	30.0%
	TCTGCAATTGAGAAAACAAA+AGG	+	chr3.2:73142237-73142256	MS.gene77579:CDS	30.0%
!	ATATCTGGAATCTTAAGAGA+AGG	-	chr3.2:73142552-73142571	None:intergenic	30.0%
	GAAAACAAAAGGTGAAACTG+TGG	+	chr3.2:73142248-73142267	MS.gene77579:CDS	35.0%
	GAAATTTGGTACATCCATGT+TGG	-	chr3.2:73142577-73142596	None:intergenic	35.0%
	AAAGGTGAAACTGTGGATGA+TGG	+	chr3.2:73142255-73142274	MS.gene77579:CDS	40.0%
	ACATCCATGTTGGTCATATC+TGG	-	chr3.2:73142567-73142586	None:intergenic	40.0%
	ATACTCCCAACGGATCTAAA+TGG	-	chr3.2:73142447-73142466	None:intergenic	40.0%
	CTCTTTGAACATACTCCCAA+CGG	-	chr3.2:73142457-73142476	None:intergenic	40.0%
	CTTAAGAGAAGGAAGAGATG+TGG	-	chr3.2:73142541-73142560	None:intergenic	40.0%
	GATTCCAGATATGACCAACA+TGG	+	chr3.2:73142560-73142579	MS.gene77579:CDS	40.0%
	GGATGTACCAAATTTCGGAA+AGG	+	chr3.2:73142581-73142600	MS.gene77579:CDS	40.0%
	TACTCCCAACGGATCTAAAT+GGG	-	chr3.2:73142446-73142465	None:intergenic	40.0%
	TTCACTTGTGATGAGAGTGA+AGG	+	chr3.2:73142407-73142426	MS.gene77579:CDS	40.0%
!	CTTTGAAGGACTAGATGCTT+CGG	-	chr3.2:73142208-73142227	None:intergenic	40.0%
!!	AAAGATGTTTCTAGCAGCCA+TGG	+	chr3.2:73142483-73142502	MS.gene77579:CDS	40.0%
	AACCACCCATTTAGATCCGT+TGG	+	chr3.2:73142438-73142457	MS.gene77579:CDS	45.0%
	AAGGACTAGATGCTTCGGAT+TGG	-	chr3.2:73142203-73142222	None:intergenic	45.0%
	ACCACCCATTTAGATCCGTT+GGG	+	chr3.2:73142439-73142458	MS.gene77579:CDS	45.0%
	AGCAATGATTGTCCTCACTG+TGG	+	chr3.2:73142365-73142384	MS.gene77579:CDS	45.0%
	AGCATCTAGTCCTTCAAAGC+AGG	+	chr3.2:73142209-73142228	MS.gene77579:CDS	45.0%
	AGGATGCATGGGAAGTGAAA+AGG	+	chr3.2:73142278-73142297	MS.gene77579:CDS	45.0%
	ATCTAGTCCTTCAAAGCAGG+TGG	+	chr3.2:73142212-73142231	MS.gene77579:CDS	45.0%
	CTTGTGATGAGAGTGAAGGA+TGG	+	chr3.2:73142411-73142430	MS.gene77579:CDS	45.0%
	ATAGTTGCCAAGCCACAGTG+AGG	-	chr3.2:73142380-73142399	None:intergenic	50.0%
	TCCCAACGGATCTAAATGGG+TGG	-	chr3.2:73142443-73142462	None:intergenic	50.0%
	TGATTGTCCTCACTGTGGCT+TGG	+	chr3.2:73142370-73142389	MS.gene77579:CDS	50.0%
	TGTCATCAGCATCCTGACCA+TGG	-	chr3.2:73142503-73142522	None:intergenic	50.0%
!	GGTGAAACTGTGGATGATGG+AGG	+	chr3.2:73142258-73142277	MS.gene77579:CDS	50.0%
!	TGTGGCTTGGCAACTATCAG+AGG	+	chr3.2:73142383-73142402	MS.gene77579:CDS	50.0%
!!	GTGGATGATGGAGGATGCAT+GGG	+	chr3.2:73142267-73142286	MS.gene77579:CDS	50.0%
!!	TGTGGATGATGGAGGATGCA+TGG	+	chr3.2:73142266-73142285	MS.gene77579:CDS	50.0%
!!	TGTTTCTAGCAGCCATGGTC+AGG	+	chr3.2:73142488-73142507	MS.gene77579:CDS	50.0%
	GCAGAAGCCACCTGCTTTGA+AGG	-	chr3.2:73142222-73142241	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	73142174	73142608	73142174	ID=MS.gene77579
chr3.2	mRNA	73142174	73142608	73142174	ID=MS.gene77579.t1;Parent=MS.gene77579
chr3.2	exon	73142174	73142608	73142174	ID=MS.gene77579.t1.exon1;Parent=MS.gene77579.t1
chr3.2	CDS	73142174	73142608	73142174	ID=cds.MS.gene77579.t1;Parent=MS.gene77579.t1

Gene Sequence

>MS.gene77579

ATGTCTAAAGGAGGTGAAACAGTTAACCAATCCGAAGCATCTAGTCCTTCAAAGCAGGTGGCTTCTGCAATTGAGAAAACAAAAGGTGAAACTGTGGATGATGGAGGATGCATGGGAAGTGAAAAGGATGAGATAATCAAAGAGCTGAAAAAGGTGAAGAAGCAGAATTTTGTGACTCATTGTCTTCTTTCAGCAATGATTGTCCTCACTGTGGCTTGGCAACTATCAGAGGTTTCACTTGTGATGAGAGTGAAGGATGGAATAAACCACCCATTTAGATCCGTTGGGAGTATGTTCAAAGAGATAGTGAAAGATGTTTCTAGCAGCCATGGTCAGGATGCTGATGACAAAGAAAACAATGAATCCACATCTCTTCCTTCTCTTAAGATTCCAGATATGACCAACATGGATGTACCAAATTTCGGAAAGGAATGA

Protein sequence

>MS.gene77579.t1

MSKGGETVNQSEASSPSKQVASAIEKTKGETVDDGGCMGSEKDEIIKELKKVKKQNFVTHCLLSAMIVLTVAWQLSEVSLVMRVKDGINHPFRSVGSMFKEIVKDVSSSHGQDADDKENNESTSLPSLKIPDMTNMDVPNFGKE