AlfalfaGEDB | MS.gene062429

M. sativa cultivar XinJiangDaYe / MS.gene062429

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062429.t1	XP_003621901.1	86.8	151	20	0	1	151	1	151	1.40E-71	278.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062429.t1	P05332	28.3	152	100	4	1	145	1	150	1.6e-07	57.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062429.t1	G7KXV4	86.8	151	20	0	1	151	1	151	1.0e-71	278.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene062429.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049150	MS.gene062429	0.806337	8.64E-50	-1.69E-46
MS.gene049260	MS.gene062429	0.802149	6.43E-49	-1.69E-46
MS.gene049297	MS.gene062429	0.814208	1.73E-51	-1.69E-46
MS.gene049346	MS.gene062429	0.807013	6.22E-50	-1.69E-46
MS.gene049758	MS.gene062429	0.825951	3.54E-54	-1.69E-46
MS.gene049910	MS.gene062429	0.806247	9.03E-50	-1.69E-46
MS.gene049915	MS.gene062429	0.819983	8.72E-53	-1.69E-46
MS.gene050226	MS.gene062429	0.813211	2.87E-51	-1.69E-46
MS.gene050227	MS.gene062429	0.816043	6.78E-52	-1.69E-46
MS.gene050228	MS.gene062429	0.81427	1.68E-51	-1.69E-46
MS.gene050301	MS.gene062429	0.803758	2.99E-49	-1.69E-46
MS.gene050330	MS.gene062429	-0.805348	1.39E-49	-1.69E-46
MS.gene050438	MS.gene062429	0.842392	2.66E-58	-1.69E-46
MS.gene050558	MS.gene062429	0.81048	1.13E-50	-1.69E-46
MS.gene050586	MS.gene062429	0.835657	1.48E-56	-1.69E-46
MS.gene050719	MS.gene062429	0.801931	7.13E-49	-1.69E-46
MS.gene050721	MS.gene062429	0.802903	4.50E-49	-1.69E-46
MS.gene051626	MS.gene062429	0.864186	1.46E-64	-1.69E-46
MS.gene051663	MS.gene062429	0.813055	3.11E-51	-1.69E-46
MS.gene051789	MS.gene062429	0.855258	7.12E-62	-1.69E-46
MS.gene051860	MS.gene062429	0.840288	9.52E-58	-1.69E-46
MS.gene052178	MS.gene062429	0.814835	1.26E-51	-1.69E-46
MS.gene052255	MS.gene062429	0.812031	5.20E-51	-1.69E-46
MS.gene052376	MS.gene062429	0.80437	2.23E-49	-1.69E-46
MS.gene052967	MS.gene062429	0.804881	1.75E-49	-1.69E-46
MS.gene053074	MS.gene062429	0.87848	2.71E-69	-1.69E-46
MS.gene053184	MS.gene062429	0.810763	9.80E-51	-1.69E-46
MS.gene053630	MS.gene062429	0.842909	1.94E-58	-1.69E-46
MS.gene053836	MS.gene062429	0.8522	5.40E-61	-1.69E-46
MS.gene054011	MS.gene062429	0.804661	1.94E-49	-1.69E-46
MS.gene054184	MS.gene062429	0.867912	9.64E-66	-1.69E-46
MS.gene054185	MS.gene062429	0.874404	6.93E-68	-1.69E-46
MS.gene054473	MS.gene062429	0.80275	4.84E-49	-1.69E-46
MS.gene054596	MS.gene062429	0.826586	2.50E-54	-1.69E-46
MS.gene054667	MS.gene062429	0.821435	4.04E-53	-1.69E-46
MS.gene054942	MS.gene062429	0.81948	1.14E-52	-1.69E-46
MS.gene054983	MS.gene062429	0.804355	2.25E-49	-1.69E-46
MS.gene055133	MS.gene062429	0.808262	3.38E-50	-1.69E-46
MS.gene055359	MS.gene062429	0.805734	1.16E-49	-1.69E-46
MS.gene055422	MS.gene062429	0.816866	4.43E-52	-1.69E-46
MS.gene055604	MS.gene062429	0.807753	4.33E-50	-1.69E-46
MS.gene056069	MS.gene062429	-0.8101	1.36E-50	-1.69E-46
MS.gene056875	MS.gene062429	0.827667	1.38E-54	-1.69E-46
MS.gene057121	MS.gene062429	0.822312	2.53E-53	-1.69E-46
MS.gene057245	MS.gene062429	0.813078	3.07E-51	-1.69E-46
MS.gene057260	MS.gene062429	0.834827	2.39E-56	-1.69E-46
MS.gene057439	MS.gene062429	0.812131	4.95E-51	-1.69E-46
MS.gene057750	MS.gene062429	0.827259	1.73E-54	-1.69E-46
MS.gene057754	MS.gene062429	0.863584	2.24E-64	-1.69E-46
MS.gene058002	MS.gene062429	0.817046	4.04E-52	-1.69E-46
MS.gene05811	MS.gene062429	0.80621	9.19E-50	-1.69E-46
MS.gene058168	MS.gene062429	0.887715	1.12E-72	-1.69E-46
MS.gene058749	MS.gene062429	0.819075	1.40E-52	-1.69E-46
MS.gene058845	MS.gene062429	0.80796	3.92E-50	-1.69E-46
MS.gene059006	MS.gene062429	0.85699	2.21E-62	-1.69E-46
MS.gene059124	MS.gene062429	0.887119	1.89E-72	-1.69E-46
MS.gene059421	MS.gene062429	0.812317	4.51E-51	-1.69E-46
MS.gene059518	MS.gene062429	0.807147	5.83E-50	-1.69E-46
MS.gene05984	MS.gene062429	0.813975	1.95E-51	-1.69E-46
MS.gene06037	MS.gene062429	0.805466	1.32E-49	-1.69E-46
MS.gene060404	MS.gene062429	0.820232	7.65E-53	-1.69E-46
MS.gene060476	MS.gene062429	0.857942	1.16E-62	-1.69E-46
MS.gene061049	MS.gene062429	0.828203	1.02E-54	-1.69E-46
MS.gene061085	MS.gene062429	0.804251	2.36E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062429.t1	MTR_7g024780	86.755	151	20	0	1	151	1	151	1.82e-96	276
MS.gene062429.t1	MTR_7g024765	68.966	145	44	1	1	145	1	144	2.20e-71	212
MS.gene062429.t1	MTR_7g024760	68.276	145	45	1	1	145	1	144	1.59e-69	207
MS.gene062429.t1	MTR_7g114410	63.014	146	53	1	1	145	1	146	2.30e-63	191
MS.gene062429.t1	MTR_7g114410	63.014	146	53	1	1	145	1	146	4.53e-63	191
MS.gene062429.t1	MTR_3g463610	52.740	146	65	2	4	145	3	148	2.17e-48	155
MS.gene062429.t1	MTR_1g103110	43.151	146	81	2	2	145	17	162	8.27e-35	120
MS.gene062429.t1	MTR_5g006470	42.759	145	81	2	3	145	16	160	1.62e-34	119
MS.gene062429.t1	MTR_1g103150	43.151	146	81	2	2	145	17	162	2.45e-33	116
MS.gene062429.t1	MTR_5g006450	39.726	146	84	2	4	145	17	162	1.74e-31	111
MS.gene062429.t1	MTR_5g006510	43.243	148	77	4	3	145	18	163	1.86e-31	111
MS.gene062429.t1	MTR_5g006460	43.709	151	75	7	3	145	17	165	4.85e-30	108
MS.gene062429.t1	MTR_5g006520	40.411	146	83	2	4	145	1735	1880	4.59e-29	111

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062429.t1	AT3G22560	45.890	146	76	2	3	145	2	147	6.32e-39	130
MS.gene062429.t1	AT2G32020	43.537	147	78	3	1	145	12	155	1.89e-37	126
MS.gene062429.t1	AT2G32030	39.437	142	81	3	6	145	22	160	4.37e-33	115

Find 40 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGAAGTATAAAGCAGATTT+TGG	0.207938	7.3:+75300884	MS.gene062429:CDS
ATGCTATTGGATTCAACTAT+TGG	0.220333	7.3:+75300909	MS.gene062429:CDS
TGCTATTGGATTCAACTATT+GGG	0.279552	7.3:+75300910	MS.gene062429:CDS
AGCTTGCAACCTTCTCAAAT+CGG	0.353792	7.3:-75300993	None:intergenic
GACATATTGATTGGCGTAAT+GGG	0.354730	7.3:-75300811	None:intergenic
ACCACTCCATTGGAATGGTT+TGG	0.355259	7.3:+75300840	MS.gene062429:CDS
TAATTCAAAGAAAGAAGCTT+TGG	0.387244	7.3:+75300763	MS.gene062429:CDS
GAGTAACTAAGCATACTAGA+TGG	0.412429	7.3:+75300732	MS.gene062429:CDS
GCTTGCAACCTTCTCAAATC+GGG	0.417621	7.3:-75300992	None:intergenic
ACGTAGATGATGTGTTATTA+TGG	0.435820	7.3:+75300696	MS.gene062429:CDS
AGACATATTGATTGGCGTAA+TGG	0.441572	7.3:-75300812	None:intergenic
TCTACGTCACTTTCCTTGAA+TGG	0.443643	7.3:-75300680	None:intergenic
AAGAATCTTCACTGCATTAG+TGG	0.453856	7.3:-75300945	None:intergenic
GGTCGAATAGTGATCCTTGA+TGG	0.461144	7.3:-75300659	None:intergenic
TTGGACAGATGAAATTCCTT+CGG	0.461234	7.3:+75301144	MS.gene062429:intron
TTCTCAAATCGGGGAACTCA+TGG	0.488747	7.3:-75300982	None:intergenic
TTCTCAGAGGGTGTTGGAAA+AGG	0.489675	7.3:+75301039	MS.gene062429:CDS
GGTCATCAAGACATATTGAT+TGG	0.500106	7.3:-75300820	None:intergenic
TGTCTTGATGACCACTCCAT+TGG	0.510495	7.3:+75300830	MS.gene062429:CDS
GGATTCAACTATTGGGGTCA+AGG	0.510777	7.3:+75300917	MS.gene062429:CDS
GATCACTATTCGACCATTCA+AGG	0.514889	7.3:+75300667	MS.gene062429:CDS
CGTAGCTTCTCAGAGGGTGT+TGG	0.532365	7.3:+75301033	MS.gene062429:CDS
TGATGACCACTCCATTGGAA+TGG	0.535636	7.3:+75300835	MS.gene062429:CDS
GCTATTGGATTCAACTATTG+GGG	0.539184	7.3:+75300911	MS.gene062429:CDS
CAGAGGGTGTTGGAAAAGGT+AGG	0.541576	7.3:+75301043	MS.gene062429:CDS
GATTCAACTATTGGGGTCAA+GGG	0.549382	7.3:+75300918	MS.gene062429:CDS
ATGAGTTCCCCGATTTGAGA+AGG	0.555146	7.3:+75300984	MS.gene062429:CDS
AGTAACTAAGCATACTAGAT+GGG	0.561128	7.3:+75300733	MS.gene062429:CDS
TGGCAATAATAAATCCATCA+AGG	0.562148	7.3:+75300645	MS.gene062429:CDS
AAGGTAGGGTTCTACAGAGA+GGG	0.585671	7.3:+75301058	MS.gene062429:CDS
AAGAATCCAAACCATTCCAA+TGG	0.587139	7.3:-75300846	None:intergenic
GATGATGTGTTATTATGGCT+AGG	0.599337	7.3:+75300701	MS.gene062429:CDS
AACGCTAACTAAACAACCGA+AGG	0.604396	7.3:-75301160	None:intergenic
TACTTCTCATCATTAGCATG+AGG	0.611961	7.3:-75300869	None:intergenic
TCCAAACCATTCCAATGGAG+TGG	0.617327	7.3:-75300841	None:intergenic
AGAGGGTGTTGGAAAAGGTA+GGG	0.622484	7.3:+75301044	MS.gene062429:CDS
CTTGCAACCTTCTCAAATCG+GGG	0.643001	7.3:-75300991	None:intergenic
AGAGAACGTAGCTTCTCAGA+GGG	0.670825	7.3:+75301027	MS.gene062429:CDS
AAGAGAACGTAGCTTCTCAG+AGG	0.683092	7.3:+75301026	MS.gene062429:CDS
AAAGGTAGGGTTCTACAGAG+AGG	0.693917	7.3:+75301057	MS.gene062429:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGAAAGGTTTTTTATTTTAA+AGG	+	chr7.3:75301088-75301107	MS.gene062429:intron	15.0%
!!!	TTTATATTTTTAGCTTTCTT+TGG	+	chr7.3:75301125-75301144	MS.gene062429:intron	15.0%
!!!	TTATTTTAAAGGAAACTTTG+TGG	+	chr7.3:75301099-75301118	MS.gene062429:intron	20.0%
!	TAATTCAAAGAAAGAAGCTT+TGG	+	chr7.3:75300763-75300782	MS.gene062429:CDS	25.0%
	AGTAACTAAGCATACTAGAT+GGG	+	chr7.3:75300733-75300752	MS.gene062429:CDS	30.0%
	ATGCTATTGGATTCAACTAT+TGG	+	chr7.3:75300909-75300928	MS.gene062429:CDS	30.0%
	TGCTATTGGATTCAACTATT+GGG	+	chr7.3:75300910-75300929	MS.gene062429:CDS	30.0%
	TGGCAATAATAAATCCATCA+AGG	+	chr7.3:75300645-75300664	MS.gene062429:CDS	30.0%
!	ACGTAGATGATGTGTTATTA+TGG	+	chr7.3:75300696-75300715	MS.gene062429:CDS	30.0%
!!	GAGAAGTATAAAGCAGATTT+TGG	+	chr7.3:75300884-75300903	MS.gene062429:CDS	30.0%
	AAGAATCCAAACCATTCCAA+TGG	-	chr7.3:75300849-75300868	None:intergenic	35.0%
	AAGAATCTTCACTGCATTAG+TGG	-	chr7.3:75300948-75300967	None:intergenic	35.0%
	GAGTAACTAAGCATACTAGA+TGG	+	chr7.3:75300732-75300751	MS.gene062429:CDS	35.0%
	GCTATTGGATTCAACTATTG+GGG	+	chr7.3:75300911-75300930	MS.gene062429:CDS	35.0%
	GGTCATCAAGACATATTGAT+TGG	-	chr7.3:75300823-75300842	None:intergenic	35.0%
	TACTTCTCATCATTAGCATG+AGG	-	chr7.3:75300872-75300891	None:intergenic	35.0%
	TTGGACAGATGAAATTCCTT+CGG	+	chr7.3:75301144-75301163	MS.gene062429:intron	35.0%
!	AGACATATTGATTGGCGTAA+TGG	-	chr7.3:75300815-75300834	None:intergenic	35.0%
!	GACATATTGATTGGCGTAAT+GGG	-	chr7.3:75300814-75300833	None:intergenic	35.0%
!	GATGATGTGTTATTATGGCT+AGG	+	chr7.3:75300701-75300720	MS.gene062429:CDS	35.0%
!!!	GCAGATTTTGGTTATGCTAT+TGG	+	chr7.3:75300896-75300915	MS.gene062429:CDS	35.0%
	AGCTTGCAACCTTCTCAAAT+CGG	-	chr7.3:75300996-75301015	None:intergenic	40.0%
	GATCACTATTCGACCATTCA+AGG	+	chr7.3:75300667-75300686	MS.gene062429:CDS	40.0%
	GATTCAACTATTGGGGTCAA+GGG	+	chr7.3:75300918-75300937	MS.gene062429:CDS	40.0%
!	TCTACGTCACTTTCCTTGAA+TGG	-	chr7.3:75300683-75300702	None:intergenic	40.0%
	AAAGGTAGGGTTCTACAGAG+AGG	+	chr7.3:75301057-75301076	MS.gene062429:CDS	45.0%
	AAGAGAACGTAGCTTCTCAG+AGG	+	chr7.3:75301026-75301045	MS.gene062429:CDS	45.0%
	AAGGTAGGGTTCTACAGAGA+GGG	+	chr7.3:75301058-75301077	MS.gene062429:CDS	45.0%
	ACCACTCCATTGGAATGGTT+TGG	+	chr7.3:75300840-75300859	MS.gene062429:CDS	45.0%
	AGAGAACGTAGCTTCTCAGA+GGG	+	chr7.3:75301027-75301046	MS.gene062429:CDS	45.0%
	CTTGCAACCTTCTCAAATCG+GGG	-	chr7.3:75300994-75301013	None:intergenic	45.0%
	GCTTGCAACCTTCTCAAATC+GGG	-	chr7.3:75300995-75301014	None:intergenic	45.0%
	GGATTCAACTATTGGGGTCA+AGG	+	chr7.3:75300917-75300936	MS.gene062429:CDS	45.0%
	GGTCGAATAGTGATCCTTGA+TGG	-	chr7.3:75300662-75300681	None:intergenic	45.0%
	TCCAAACCATTCCAATGGAG+TGG	-	chr7.3:75300844-75300863	None:intergenic	45.0%
	TGATGACCACTCCATTGGAA+TGG	+	chr7.3:75300835-75300854	MS.gene062429:CDS	45.0%
	TTCTCAAATCGGGGAACTCA+TGG	-	chr7.3:75300985-75301004	None:intergenic	45.0%
!	ATGAGTTCCCCGATTTGAGA+AGG	+	chr7.3:75300984-75301003	MS.gene062429:CDS	45.0%
!	TGTCTTGATGACCACTCCAT+TGG	+	chr7.3:75300830-75300849	MS.gene062429:CDS	45.0%
!!	AGAGGGTGTTGGAAAAGGTA+GGG	+	chr7.3:75301044-75301063	MS.gene062429:CDS	45.0%
!!	TTCTCAGAGGGTGTTGGAAA+AGG	+	chr7.3:75301039-75301058	MS.gene062429:CDS	45.0%
!	CAGAGAGGGTATGCTCAGAA+AGG	+	chr7.3:75301072-75301091	MS.gene062429:intron	50.0%
!!	CAGAGGGTGTTGGAAAAGGT+AGG	+	chr7.3:75301043-75301062	MS.gene062429:CDS	50.0%
!	CGTAGCTTCTCAGAGGGTGT+TGG	+	chr7.3:75301033-75301052	MS.gene062429:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	75300644	75301174	75300644	ID=MS.gene062429
chr7.3	mRNA	75300644	75301174	75300644	ID=MS.gene062429.t1;Parent=MS.gene062429
chr7.3	exon	75300644	75301079	75300644	ID=MS.gene062429.t1.exon1;Parent=MS.gene062429.t1
chr7.3	CDS	75300644	75301079	75300644	ID=cds.MS.gene062429.t1;Parent=MS.gene062429.t1
chr7.3	exon	75301152	75301174	75301152	ID=MS.gene062429.t1.exon2;Parent=MS.gene062429.t1
chr7.3	CDS	75301152	75301174	75301152	ID=cds.MS.gene062429.t1;Parent=MS.gene062429.t1

Gene Sequence

>MS.gene062429

ATGGCAATAATAAATCCATCAAGGATCACTATTCGACCATTCAAGGAAAGTGACGTAGATGATGTGTTATTATGGCTAGGAGATGAAAGAGTAACTAAGCATACTAGATGGGAAACATGTAATTCAAAGAAAGAAGCTTTGGATTTCATAAAATATGAATGCATTTACCCATTACGCCAATCAATATGTCTTGATGACCACTCCATTGGAATGGTTTGGATTCTTCCTCATGCTAATGATGAGAAGTATAAAGCAGATTTTGGTTATGCTATTGGATTCAACTATTGGGGTCAAGGGATTGCCACTAATGCAGTGAAGATTCTTCTGTCAAAAGTGTTCCATGAGTTCCCCGATTTGAGAAGGTTGCAAGCTTTCACTGTTAAAGAGAACGTAGCTTCTCAGAGGGTGTTGGAAAAGGTAGGGTTCTACAGAGAGGGTATGCTCAGAAAGGTTTTTTATTTTAAAGGAAACTTTGTGGATTTTTATATTTTTAGCTTTCTTTGGACAGATGAAATTCCTTCGGTTGTTTAG

Protein sequence

>MS.gene062429.t1

MAIINPSRITIRPFKESDVDDVLLWLGDERVTKHTRWETCNSKKEALDFIKYECIYPLRQSICLDDHSIGMVWILPHANDEKYKADFGYAIGFNYWGQGIATNAVKILLSKVFHEFPDLRRLQAFTVKENVASQRVLEKVGFYREDEIPSVV