Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06465.t1 | RHN69864.1 | 98.8 | 85 | 1 | 0 | 1 | 85 | 35 | 119 | 3.40E-38 | 167.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06465.t1 | G7JAD2 | 98.8 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 2.4e-38 | 167.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049346 | MS.gene06465 | 0.999927 | 0.00E+00 | -1.69E-46 |
| MS.gene049661 | MS.gene06465 | 0.800119 | 1.67E-48 | -1.69E-46 |
| MS.gene049758 | MS.gene06465 | 0.844234 | 8.58E-59 | -1.69E-46 |
| MS.gene049910 | MS.gene06465 | 0.828257 | 9.94E-55 | -1.69E-46 |
| MS.gene049915 | MS.gene06465 | 0.841341 | 5.04E-58 | -1.69E-46 |
| MS.gene050301 | MS.gene06465 | 0.8408 | 6.99E-58 | -1.69E-46 |
| MS.gene050397 | MS.gene06465 | 0.818533 | 1.86E-52 | -1.69E-46 |
| MS.gene050406 | MS.gene06465 | 0.835481 | 1.64E-56 | -1.69E-46 |
| MS.gene050558 | MS.gene06465 | 0.809756 | 1.62E-50 | -1.69E-46 |
| MS.gene051582 | MS.gene06465 | 0.827441 | 1.56E-54 | -1.69E-46 |
| MS.gene051860 | MS.gene06465 | 0.808226 | 3.44E-50 | -1.69E-46 |
| MS.gene052011 | MS.gene06465 | -0.815449 | 9.19E-52 | -1.69E-46 |
| MS.gene052253 | MS.gene06465 | 0.837364 | 5.43E-57 | -1.69E-46 |
| MS.gene052255 | MS.gene06465 | 0.812849 | 3.45E-51 | -1.69E-46 |
| MS.gene052340 | MS.gene06465 | 0.849729 | 2.68E-60 | -1.69E-46 |
| MS.gene053209 | MS.gene06465 | 0.812873 | 3.41E-51 | -1.69E-46 |
| MS.gene053557 | MS.gene06465 | 0.803359 | 3.62E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene06465 | 0.871478 | 6.63E-67 | -1.69E-46 |
| MS.gene053928 | MS.gene06465 | 0.814937 | 1.19E-51 | -1.69E-46 |
| MS.gene053971 | MS.gene06465 | 0.802529 | 5.37E-49 | -1.69E-46 |
| MS.gene054473 | MS.gene06465 | 0.852335 | 4.94E-61 | -1.69E-46 |
| MS.gene054542 | MS.gene06465 | 0.82228 | 2.58E-53 | -1.69E-46 |
| MS.gene054930 | MS.gene06465 | 0.801639 | 8.19E-49 | -1.69E-46 |
| MS.gene054983 | MS.gene06465 | 0.836284 | 1.02E-56 | -1.69E-46 |
| MS.gene055116 | MS.gene06465 | 0.848864 | 4.67E-60 | -1.69E-46 |
| MS.gene055277 | MS.gene06465 | 0.81452 | 1.48E-51 | -1.69E-46 |
| MS.gene055422 | MS.gene06465 | 0.815698 | 8.09E-52 | -1.69E-46 |
| MS.gene055428 | MS.gene06465 | 0.827863 | 1.24E-54 | -1.69E-46 |
| MS.gene05553 | MS.gene06465 | 0.816978 | 4.19E-52 | -1.69E-46 |
| MS.gene056191 | MS.gene06465 | 0.843174 | 1.65E-58 | -1.69E-46 |
| MS.gene056407 | MS.gene06465 | 0.804846 | 1.78E-49 | -1.69E-46 |
| MS.gene056612 | MS.gene06465 | 0.817762 | 2.79E-52 | -1.69E-46 |
| MS.gene056723 | MS.gene06465 | 0.809248 | 2.08E-50 | -1.69E-46 |
| MS.gene05702 | MS.gene06465 | 0.852492 | 4.45E-61 | -1.69E-46 |
| MS.gene057121 | MS.gene06465 | 0.822329 | 2.51E-53 | -1.69E-46 |
| MS.gene05733 | MS.gene06465 | 0.828196 | 1.03E-54 | -1.69E-46 |
| MS.gene057450 | MS.gene06465 | 0.801575 | 8.44E-49 | -1.69E-46 |
| MS.gene057754 | MS.gene06465 | 0.823422 | 1.40E-53 | -1.69E-46 |
| MS.gene057782 | MS.gene06465 | 0.808422 | 3.12E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene06465 | 0.851738 | 7.30E-61 | -1.69E-46 |
| MS.gene05811 | MS.gene06465 | 0.833379 | 5.52E-56 | -1.69E-46 |
| MS.gene058142 | MS.gene06465 | 0.809654 | 1.70E-50 | -1.69E-46 |
| MS.gene058233 | MS.gene06465 | 0.80926 | 2.07E-50 | -1.69E-46 |
| MS.gene058236 | MS.gene06465 | 0.807035 | 6.15E-50 | -1.69E-46 |
| MS.gene058710 | MS.gene06465 | 0.815328 | 9.78E-52 | -1.69E-46 |
| MS.gene058749 | MS.gene06465 | 0.805483 | 1.31E-49 | -1.69E-46 |
| MS.gene059011 | MS.gene06465 | 0.801874 | 7.33E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene06465 | 0.836394 | 9.60E-57 | -1.69E-46 |
| MS.gene059124 | MS.gene06465 | 0.80957 | 1.77E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene06465 | 0.820473 | 6.73E-53 | -1.69E-46 |
| MS.gene059421 | MS.gene06465 | 0.817552 | 3.11E-52 | -1.69E-46 |
| MS.gene059518 | MS.gene06465 | 0.802806 | 4.71E-49 | -1.69E-46 |
| MS.gene059688 | MS.gene06465 | 0.821413 | 4.09E-53 | -1.69E-46 |
| MS.gene059692 | MS.gene06465 | 0.844078 | 9.44E-59 | -1.69E-46 |
| MS.gene059695 | MS.gene06465 | 0.841812 | 3.79E-58 | -1.69E-46 |
| MS.gene05984 | MS.gene06465 | 0.833885 | 4.12E-56 | -1.69E-46 |
| MS.gene059894 | MS.gene06465 | 0.839899 | 1.20E-57 | -1.69E-46 |
| MS.gene06037 | MS.gene06465 | 0.848402 | 6.27E-60 | -1.69E-46 |
| MS.gene060476 | MS.gene06465 | 0.822492 | 2.30E-53 | -1.69E-46 |
| MS.gene060986 | MS.gene06465 | 0.806644 | 7.44E-50 | -1.69E-46 |
| MS.gene061049 | MS.gene06465 | 0.89161 | 3.40E-74 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06465.t1 | MTR_3g095180 | 98.824 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 6.75e-59 | 174 |
| MS.gene06465.t1 | MTR_8g092340 | 52.941 | 85 | 32 | 4 | 1 | 85 | 1 | 77 | 5.49e-21 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCATCCATATTCAAGTTAA+AGG | 0.155938 | 3.2:+79550540 | MS.gene06465:CDS |
| AGGTACCTTTAACTTGAATA+TGG | 0.246398 | 3.2:-79550545 | None:intergenic |
| TGCACTATTTAATTGTGAAT+TGG | 0.396593 | 3.2:-79550840 | None:intergenic |
| ATTGGTGGTTTCCTTGATGC+TGG | 0.439868 | 3.2:-79550822 | None:intergenic |
| TGTTGACATCCTCACTACTT+TGG | 0.458998 | 3.2:-79550704 | None:intergenic |
| CAACAAAGCTCAACAACATA+AGG | 0.461599 | 3.2:+79550722 | MS.gene06465:CDS |
| ACCTTTAACTTGAATATGGA+TGG | 0.490545 | 3.2:-79550541 | None:intergenic |
| ACTATTTAATTGTGAATTGG+TGG | 0.515565 | 3.2:-79550837 | None:intergenic |
| GACATGTTTAAGCTAAAAGA+AGG | 0.527331 | 3.2:+79550756 | MS.gene06465:CDS |
| TATGGATGGAGAAGAAGCAA+AGG | 0.568511 | 3.2:-79550527 | None:intergenic |
| TGGATTATACTCCAGCATCA+AGG | 0.596772 | 3.2:+79550811 | MS.gene06465:CDS |
| AATAAACAACCAAAGTAGTG+AGG | 0.645718 | 3.2:+79550695 | MS.gene06465:CDS |
| CAAAGCTCAACAACATAAGG+AGG | 0.647677 | 3.2:+79550725 | MS.gene06465:CDS |
| ATGTTTAAGCTAAAAGAAGG+AGG | 0.667536 | 3.2:+79550759 | MS.gene06465:CDS |
| AAAGAAGGAGGAGATAACGT+TGG | 0.669136 | 3.2:+79550771 | MS.gene06465:CDS |
| TGATGCTGGAGTATAATCCA+TGG | 0.719915 | 3.2:-79550808 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAAACAGAAAATAAAAA+TGG | - | chr3.2:79550628-79550647 | None:intergenic | 10.0% |
| !! | TAAAAAAAATATTACAACAT+TGG | + | chr3.2:79550589-79550608 | MS.gene06465:intron | 10.0% |
| !!! | AATTTTTCTTACATTTTTTT+TGG | + | chr3.2:79550648-79550667 | MS.gene06465:intron | 10.0% |
| !!! | TGTAATATTTTTTTTAGCTA+TGG | - | chr3.2:79550585-79550604 | None:intergenic | 15.0% |
| AATAAACAACCAAAGTAGTG+AGG | + | chr3.2:79550695-79550714 | MS.gene06465:CDS | 30.0% | |
| ATGTTTAAGCTAAAAGAAGG+AGG | + | chr3.2:79550759-79550778 | MS.gene06465:CDS | 30.0% | |
| GACATGTTTAAGCTAAAAGA+AGG | + | chr3.2:79550756-79550775 | MS.gene06465:CDS | 30.0% | |
| ! | CTATGGATATTTGAAGCAAA+AGG | - | chr3.2:79550568-79550587 | None:intergenic | 30.0% |
| ! | TCCATCCATATTCAAGTTAA+AGG | + | chr3.2:79550540-79550559 | MS.gene06465:CDS | 30.0% |
| !! | ACCTTTAACTTGAATATGGA+TGG | - | chr3.2:79550544-79550563 | None:intergenic | 30.0% |
| !! | AGGTACCTTTAACTTGAATA+TGG | - | chr3.2:79550548-79550567 | None:intergenic | 30.0% |
| CAACAAAGCTCAACAACATA+AGG | + | chr3.2:79550722-79550741 | MS.gene06465:CDS | 35.0% | |
| AAAGAAGGAGGAGATAACGT+TGG | + | chr3.2:79550771-79550790 | MS.gene06465:CDS | 40.0% | |
| CAAAGCTCAACAACATAAGG+AGG | + | chr3.2:79550725-79550744 | MS.gene06465:CDS | 40.0% | |
| TATGGATGGAGAAGAAGCAA+AGG | - | chr3.2:79550530-79550549 | None:intergenic | 40.0% | |
| TGATGCTGGAGTATAATCCA+TGG | - | chr3.2:79550811-79550830 | None:intergenic | 40.0% | |
| TGGATTATACTCCAGCATCA+AGG | + | chr3.2:79550811-79550830 | MS.gene06465:CDS | 40.0% | |
| TGTTGACATCCTCACTACTT+TGG | - | chr3.2:79550707-79550726 | None:intergenic | 40.0% | |
| !!! | TGGTTCTGATGTTTTCACCA+TGG | + | chr3.2:79550791-79550810 | MS.gene06465:CDS | 40.0% |
| ! | ATTGGTGGTTTCCTTGATGC+TGG | - | chr3.2:79550825-79550844 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 79550480 | 79550854 | 79550480 | ID=MS.gene06465 |
| chr3.2 | mRNA | 79550480 | 79550854 | 79550480 | ID=MS.gene06465.t1;Parent=MS.gene06465 |
| chr3.2 | exon | 79550480 | 79550561 | 79550480 | ID=MS.gene06465.t1.exon1;Parent=MS.gene06465.t1 |
| chr3.2 | CDS | 79550480 | 79550561 | 79550480 | ID=cds.MS.gene06465.t1;Parent=MS.gene06465.t1 |
| chr3.2 | exon | 79550679 | 79550854 | 79550679 | ID=MS.gene06465.t1.exon2;Parent=MS.gene06465.t1 |
| chr3.2 | CDS | 79550679 | 79550854 | 79550679 | ID=cds.MS.gene06465.t1;Parent=MS.gene06465.t1 |
| Gene Sequence |
| Protein sequence |
